AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0612290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0612290 MtrunA17_Chr4g0045981 98.230 113 2 0 1 113 517 629 9.94e-77 238
Msa0612290 MtrunA17_Chr1g0198861 57.000 100 37 2 2 98 874 970 4.74e-31 115
Msa0612290 MtrunA17_Chr5g0422391 55.208 96 40 1 2 94 886 981 1.00e-29 111
Msa0612290 MtrunA17_Chr1g0151531 57.447 94 37 1 2 92 655 748 1.01e-28 108
Msa0612290 MtrunA17_Chr5g0430701 46.667 90 47 1 1 89 328 417 1.45e-23 93.6
Msa0612290 MtrunA17_Chr4g0069431 42.553 94 51 1 1 91 463 556 3.38e-22 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0612290 AT4G18570.1 72.414 116 29 2 1 113 527 642 7.83e-47 159
Msa0612290 AT3G25690.5 60.417 96 35 1 2 94 891 986 1.86e-32 119
Msa0612290 AT3G25690.1 60.417 96 35 1 2 94 889 984 2.07e-32 119
Msa0612290 AT3G25690.2 60.417 96 35 1 2 94 889 984 2.07e-32 119
Msa0612290 AT3G25690.6 60.417 96 35 1 2 94 889 984 2.07e-32 119
Msa0612290 AT3G25690.4 60.417 96 35 1 2 94 889 984 2.07e-32 119
Msa0612290 AT3G25690.3 60.417 96 35 1 2 94 748 843 2.69e-32 119
Msa0612290 AT1G07120.1 51.685 89 42 1 1 89 296 383 4.02e-27 103
Msa0612290 AT1G48280.1 46.392 97 49 1 1 94 448 544 2.47e-22 90.9

Find 36 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACTAATTTAATTATTTC+TGG 0.099407 4_2:+60726928 None:intergenic
TATTCACAAACTAGTGAAAA+AGG 0.219685 4_2:+60726900 None:intergenic
TGGCTTTATCTCTCAATTCT+TGG 0.255039 4_2:+60727035 None:intergenic
GCAACCAATCTACAGGTATT+TGG 0.272044 4_2:+60728025 None:intergenic
AAAGCTGGCATCTGTAAAAT+TGG 0.288625 4_2:-60727325 Msa0612290:CDS
TCACTAGTTTGTGAATATAA+TGG 0.291207 4_2:-60726894 Msa0612290:three_prime_UTR
ACAATGAGCTCTTCTTCTTC+AGG 0.305449 4_2:+60727249 None:intergenic
GCTTGTTCTTGTGAATTATC+TGG 0.328841 4_2:-60726764 Msa0612290:three_prime_UTR
AATGTATTCCACTTTAATGA+TGG 0.388126 4_2:+60726852 None:intergenic
TCATTCATTTATAAAGAAAC+AGG 0.406634 4_2:-60726721 Msa0612290:three_prime_UTR
ATTTGCTTTCCGTGTACATC+AGG 0.429708 4_2:-60727214 Msa0612290:intron
TCTTCCAAATACCTGTAGAT+TGG 0.435132 4_2:-60728029 Msa0612290:CDS
AAATTAATTTGCTTCCACTT+TGG 0.442448 4_2:-60726788 Msa0612290:three_prime_UTR
GAGCTTGAAACTGTTGGTGG+TGG 0.454165 4_2:-60727273 Msa0612290:CDS
CTTAATTAAAATTACCTTGG+TGG 0.459160 4_2:+60727987 None:intergenic
AGCTTGAAACTGTTGGTGGT+GGG 0.479377 4_2:-60727272 Msa0612290:CDS
TCTGCTGAGCTTGAAACTGT+TGG 0.494822 4_2:-60727279 Msa0612290:CDS
GGAAAGCAAATCTGACTCCT+TGG 0.497658 4_2:+60727226 None:intergenic
TTGCTCCTGCATTAACATGC+TGG 0.514086 4_2:+60726959 None:intergenic
AAGAATTGAGAGATAAAGCC+AGG 0.519809 4_2:-60727033 Msa0612290:CDS
GCTGAGCTTGAAACTGTTGG+TGG 0.524244 4_2:-60727276 Msa0612290:CDS
GGCCTCCAGCATGTTAATGC+AGG 0.528236 4_2:-60726964 Msa0612290:exon
CTATCAAGCAACCAATCTAC+AGG 0.536269 4_2:+60728018 None:intergenic
TATTCCACTTTAATGATGGA+TGG 0.537798 4_2:+60726856 None:intergenic
AATTGTGTTATAGATAAAGC+TGG 0.545820 4_2:-60727340 Msa0612290:intron
TCATGTCATGTTCAATGCCA+TGG 0.550049 4_2:-60727010 Msa0612290:CDS
GAAGAAGAGCTCATTGTCCA+AGG 0.553034 4_2:-60727243 Msa0612290:CDS
CACAAGAACAAGCGCCAAAG+TGG 0.567622 4_2:+60726774 None:intergenic
TTAGCCATCCATCATTAAAG+TGG 0.569376 4_2:-60726860 Msa0612290:three_prime_UTR
CTCCTGCATTAACATGCTGG+AGG 0.576851 4_2:+60726962 None:intergenic
GTAGATTGGTTGCTTGATAG+CGG 0.595636 4_2:-60728015 Msa0612290:CDS
TTACTGATTTGTTTAACGAA+TGG 0.596856 4_2:+60726813 None:intergenic
GATGATGGTGCAATTGTCCA+TGG 0.632629 4_2:+60726612 None:intergenic
GCATTGAACATGACATGACC+TGG 0.668619 4_2:+60727015 None:intergenic
TCATTTATAAAGAAACAGGA+CGG 0.688304 4_2:-60726717 Msa0612290:three_prime_UTR
TGATAGCGGTTATGCCACCA+AGG 0.695302 4_2:-60728001 Msa0612290:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGAAACAAAAAAAAA+AGG + chr4_2:60727288-60727307 None:intergenic 10.0%
!! TATACTAATTTAATTATTTC+TGG + chr4_2:60727786-60727805 None:intergenic 10.0%
!! AAATATGAAACAAAAAAAAA+AGG + chr4_2:60727288-60727307 None:intergenic 10.0%
!! TATACTAATTTAATTATTTC+TGG + chr4_2:60727786-60727805 None:intergenic 10.0%
!! AAAATAATAAACAGTGATAA+AGG + chr4_2:60726752-60726771 None:intergenic 15.0%
!!! ATGAATTGATGTTATTTTAT+TGG - chr4_2:60726855-60726874 Msa0612290:three_prime_UTR 15.0%
!!! AATTTTAAAGATAAGCATAT+AGG + chr4_2:60727224-60727243 None:intergenic 15.0%
!!! TTTTGTTTCATATTTAATCA+CGG - chr4_2:60727293-60727312 Msa0612290:CDS 15.0%
!! AAAATAATAAACAGTGATAA+AGG + chr4_2:60726752-60726771 None:intergenic 15.0%
!!! ATGAATTGATGTTATTTTAT+TGG - chr4_2:60726855-60726874 Msa0612290:three_prime_UTR 15.0%
!!! AATTTTAAAGATAAGCATAT+AGG + chr4_2:60727224-60727243 None:intergenic 15.0%
!!! TTTTGTTTCATATTTAATCA+CGG - chr4_2:60727293-60727312 Msa0612290:CDS 15.0%
!! GAACTTAATTAAAATTACCT+TGG + chr4_2:60726730-60726749 None:intergenic 20.0%
!!! TCAACCAGTTTATTATATTT+TGG - chr4_2:60726820-60726839 Msa0612290:three_prime_UTR 20.0%
!! TGATCCAAAATATAATAAAC+TGG + chr4_2:60726827-60726846 None:intergenic 20.0%
!! GAAGATTAATATTTACGAAA+GGG + chr4_2:60726896-60726915 None:intergenic 20.0%
!! AGAAGATTAATATTTACGAA+AGG + chr4_2:60726897-60726916 None:intergenic 20.0%
!!! AAGGGAAACTATATTTTATA+AGG - chr4_2:60727182-60727201 Msa0612290:intron 20.0%
!! TCATTCATTTATAAAGAAAC+AGG - chr4_2:60727990-60728009 Msa0612290:intron 20.0%
!! GAACTTAATTAAAATTACCT+TGG + chr4_2:60726730-60726749 None:intergenic 20.0%
!!! TCAACCAGTTTATTATATTT+TGG - chr4_2:60726820-60726839 Msa0612290:three_prime_UTR 20.0%
!! TGATCCAAAATATAATAAAC+TGG + chr4_2:60726827-60726846 None:intergenic 20.0%
!! GAAGATTAATATTTACGAAA+GGG + chr4_2:60726896-60726915 None:intergenic 20.0%
!! AGAAGATTAATATTTACGAA+AGG + chr4_2:60726897-60726916 None:intergenic 20.0%
!!! AAGGGAAACTATATTTTATA+AGG - chr4_2:60727182-60727201 Msa0612290:intron 20.0%
!! TCATTCATTTATAAAGAAAC+AGG - chr4_2:60727990-60728009 Msa0612290:intron 20.0%
! CTTAATTAAAATTACCTTGG+TGG + chr4_2:60726727-60726746 None:intergenic 25.0%
!!! TTTTATTGGTTGATGTTGAA+TGG - chr4_2:60726869-60726888 Msa0612290:three_prime_UTR 25.0%
!! TTTATTGGTTGATGTTGAAT+GGG - chr4_2:60726870-60726889 Msa0612290:three_prime_UTR 25.0%
! AATTGTGTTATAGATAAAGC+TGG - chr4_2:60727371-60727390 Msa0612290:intron 25.0%
!! AAATTTTTACCTGATGTACA+CGG + chr4_2:60727509-60727528 None:intergenic 25.0%
!!! GAGTAATGTGCTATTATTTT+TGG - chr4_2:60727612-60727631 Msa0612290:intron 25.0%
! ATTCACAAACTAGTGAAAAA+GGG + chr4_2:60727813-60727832 None:intergenic 25.0%
! TATTCACAAACTAGTGAAAA+AGG + chr4_2:60727814-60727833 None:intergenic 25.0%
!! TCACTAGTTTGTGAATATAA+TGG - chr4_2:60727817-60727836 Msa0612290:intron 25.0%
! AATGTATTCCACTTTAATGA+TGG + chr4_2:60727862-60727881 None:intergenic 25.0%
!!! TTACTGATTTGTTTAACGAA+TGG + chr4_2:60727901-60727920 None:intergenic 25.0%
!!! AAATTAATTTGCTTCCACTT+TGG - chr4_2:60727923-60727942 Msa0612290:intron 25.0%
! TCATTTATAAAGAAACAGGA+CGG - chr4_2:60727994-60728013 Msa0612290:intron 25.0%
!!! TTGTTTATTTTCATTTGCCA+TGG - chr4_2:60728082-60728101 Msa0612290:exon 25.0%
! CTTAATTAAAATTACCTTGG+TGG + chr4_2:60726727-60726746 None:intergenic 25.0%
!!! TTTTATTGGTTGATGTTGAA+TGG - chr4_2:60726869-60726888 Msa0612290:three_prime_UTR 25.0%
!! TTTATTGGTTGATGTTGAAT+GGG - chr4_2:60726870-60726889 Msa0612290:three_prime_UTR 25.0%
! AATTGTGTTATAGATAAAGC+TGG - chr4_2:60727371-60727390 Msa0612290:intron 25.0%
!! AAATTTTTACCTGATGTACA+CGG + chr4_2:60727509-60727528 None:intergenic 25.0%
!!! GAGTAATGTGCTATTATTTT+TGG - chr4_2:60727612-60727631 Msa0612290:intron 25.0%
! ATTCACAAACTAGTGAAAAA+GGG + chr4_2:60727813-60727832 None:intergenic 25.0%
! TATTCACAAACTAGTGAAAA+AGG + chr4_2:60727814-60727833 None:intergenic 25.0%
!! TCACTAGTTTGTGAATATAA+TGG - chr4_2:60727817-60727836 Msa0612290:intron 25.0%
! AATGTATTCCACTTTAATGA+TGG + chr4_2:60727862-60727881 None:intergenic 25.0%
!!! TTACTGATTTGTTTAACGAA+TGG + chr4_2:60727901-60727920 None:intergenic 25.0%
!!! AAATTAATTTGCTTCCACTT+TGG - chr4_2:60727923-60727942 Msa0612290:intron 25.0%
! TCATTTATAAAGAAACAGGA+CGG - chr4_2:60727994-60728013 Msa0612290:intron 25.0%
!!! TTGTTTATTTTCATTTGCCA+TGG - chr4_2:60728082-60728101 Msa0612290:exon 25.0%
TTTGAGTACTATAGACAAGT+TGG + chr4_2:60726935-60726954 None:intergenic 30.0%
TGCTATAAGAAACTTTACCA+TGG + chr4_2:60726965-60726984 None:intergenic 30.0%
TAATAGATTGTGGAGATCTT+GGG + chr4_2:60727109-60727128 None:intergenic 30.0%
TTTCATATTTAATCACGGTG+AGG - chr4_2:60727298-60727317 Msa0612290:CDS 30.0%
TGAGGTGAAATCTCAAATAT+TGG - chr4_2:60727316-60727335 Msa0612290:CDS 30.0%
! TATTCCACTTTAATGATGGA+TGG + chr4_2:60727858-60727877 None:intergenic 30.0%
TTTGAGTACTATAGACAAGT+TGG + chr4_2:60726935-60726954 None:intergenic 30.0%
TGCTATAAGAAACTTTACCA+TGG + chr4_2:60726965-60726984 None:intergenic 30.0%
TAATAGATTGTGGAGATCTT+GGG + chr4_2:60727109-60727128 None:intergenic 30.0%
TTTCATATTTAATCACGGTG+AGG - chr4_2:60727298-60727317 Msa0612290:CDS 30.0%
TGAGGTGAAATCTCAAATAT+TGG - chr4_2:60727316-60727335 Msa0612290:CDS 30.0%
! TATTCCACTTTAATGATGGA+TGG + chr4_2:60727858-60727877 None:intergenic 30.0%
TCTTCCAAATACCTGTAGAT+TGG - chr4_2:60726682-60726701 Msa0612290:three_prime_UTR 35.0%
! GTTTTCACGAACGAGAAAAA+TGG - chr4_2:60726779-60726798 Msa0612290:three_prime_UTR 35.0%
TGCTGATAAATTTCATCCTC+TGG - chr4_2:60727037-60727056 Msa0612290:CDS 35.0%
ATTGAAAACACAGAAACCAG+AGG + chr4_2:60727056-60727075 None:intergenic 35.0%
AGATCTTGGGTATCTATCTA+GGG + chr4_2:60727096-60727115 None:intergenic 35.0%
CTAATAGATTGTGGAGATCT+TGG + chr4_2:60727110-60727129 None:intergenic 35.0%
CAAGATCTCCACAATCTATT+AGG - chr4_2:60727108-60727127 Msa0612290:intron 35.0%
CATACAGTCCTAATAGATTG+TGG + chr4_2:60727119-60727138 None:intergenic 35.0%
AGAAGGAGATTCAACTTTGA+AGG - chr4_2:60727163-60727182 Msa0612290:intron 35.0%
GAAGGAGATTCAACTTTGAA+GGG - chr4_2:60727164-60727183 Msa0612290:intron 35.0%
AAAGCTGGCATCTGTAAAAT+TGG - chr4_2:60727386-60727405 Msa0612290:intron 35.0%
TGCCTATACAACCATTGTAA+AGG - chr4_2:60727559-60727578 Msa0612290:intron 35.0%
GTCCTTTACAATGGTTGTAT+AGG + chr4_2:60727564-60727583 None:intergenic 35.0%
TAATAGCACATTACTCAGAG+TGG + chr4_2:60727608-60727627 None:intergenic 35.0%
!!! TATTTTTGGCAGTTTGCTAG+CGG - chr4_2:60727626-60727645 Msa0612290:intron 35.0%
!! TGGCTTTATCTCTCAATTCT+TGG + chr4_2:60727679-60727698 None:intergenic 35.0%
AAGAATTGAGAGATAAAGCC+AGG - chr4_2:60727678-60727697 Msa0612290:intron 35.0%
! TGCACAAGAATTTTTGTTGC+TGG + chr4_2:60727727-60727746 None:intergenic 35.0%
AGCAACAAAAATTCTTGTGC+AGG - chr4_2:60727726-60727745 Msa0612290:intron 35.0%
TTAGCCATCCATCATTAAAG+TGG - chr4_2:60727851-60727870 Msa0612290:intron 35.0%
GCTTGTTCTTGTGAATTATC+TGG - chr4_2:60727947-60727966 Msa0612290:intron 35.0%
TCTTCCAAATACCTGTAGAT+TGG - chr4_2:60726682-60726701 Msa0612290:three_prime_UTR 35.0%
! GTTTTCACGAACGAGAAAAA+TGG - chr4_2:60726779-60726798 Msa0612290:three_prime_UTR 35.0%
TGCTGATAAATTTCATCCTC+TGG - chr4_2:60727037-60727056 Msa0612290:CDS 35.0%
ATTGAAAACACAGAAACCAG+AGG + chr4_2:60727056-60727075 None:intergenic 35.0%
AGATCTTGGGTATCTATCTA+GGG + chr4_2:60727096-60727115 None:intergenic 35.0%
CTAATAGATTGTGGAGATCT+TGG + chr4_2:60727110-60727129 None:intergenic 35.0%
CAAGATCTCCACAATCTATT+AGG - chr4_2:60727108-60727127 Msa0612290:intron 35.0%
CATACAGTCCTAATAGATTG+TGG + chr4_2:60727119-60727138 None:intergenic 35.0%
AGAAGGAGATTCAACTTTGA+AGG - chr4_2:60727163-60727182 Msa0612290:intron 35.0%
GAAGGAGATTCAACTTTGAA+GGG - chr4_2:60727164-60727183 Msa0612290:intron 35.0%
AAAGCTGGCATCTGTAAAAT+TGG - chr4_2:60727386-60727405 Msa0612290:intron 35.0%
TGCCTATACAACCATTGTAA+AGG - chr4_2:60727559-60727578 Msa0612290:intron 35.0%
GTCCTTTACAATGGTTGTAT+AGG + chr4_2:60727564-60727583 None:intergenic 35.0%
TAATAGCACATTACTCAGAG+TGG + chr4_2:60727608-60727627 None:intergenic 35.0%
!!! TATTTTTGGCAGTTTGCTAG+CGG - chr4_2:60727626-60727645 Msa0612290:intron 35.0%
!! TGGCTTTATCTCTCAATTCT+TGG + chr4_2:60727679-60727698 None:intergenic 35.0%
AAGAATTGAGAGATAAAGCC+AGG - chr4_2:60727678-60727697 Msa0612290:intron 35.0%
! TGCACAAGAATTTTTGTTGC+TGG + chr4_2:60727727-60727746 None:intergenic 35.0%
AGCAACAAAAATTCTTGTGC+AGG - chr4_2:60727726-60727745 Msa0612290:intron 35.0%
TTAGCCATCCATCATTAAAG+TGG - chr4_2:60727851-60727870 Msa0612290:intron 35.0%
GCTTGTTCTTGTGAATTATC+TGG - chr4_2:60727947-60727966 Msa0612290:intron 35.0%
GCAACCAATCTACAGGTATT+TGG + chr4_2:60726689-60726708 None:intergenic 40.0%
CTATCAAGCAACCAATCTAC+AGG + chr4_2:60726696-60726715 None:intergenic 40.0%
! GTAGATTGGTTGCTTGATAG+CGG - chr4_2:60726696-60726715 Msa0612290:three_prime_UTR 40.0%
AGTACTCAAAACAGTAGCCA+TGG - chr4_2:60726945-60726964 Msa0612290:three_prime_UTR 40.0%
GAGATCTTGGGTATCTATCT+AGG + chr4_2:60727097-60727116 None:intergenic 40.0%
!! CTGCTTGGTCTTTTTAGAGA+AGG - chr4_2:60727146-60727165 Msa0612290:intron 40.0%
ACAATGAGCTCTTCTTCTTC+AGG + chr4_2:60727465-60727484 None:intergenic 40.0%
ATTTGCTTTCCGTGTACATC+AGG - chr4_2:60727497-60727516 Msa0612290:intron 40.0%
CTCAGAATGGTCCTTTACAA+TGG + chr4_2:60727573-60727592 None:intergenic 40.0%
GACCATTCTGAGCAATGTAA+AGG - chr4_2:60727581-60727600 Msa0612290:intron 40.0%
GTCCTTTACATTGCTCAGAA+TGG + chr4_2:60727586-60727605 None:intergenic 40.0%
TCATGTCATGTTCAATGCCA+TGG - chr4_2:60727701-60727720 Msa0612290:intron 40.0%
! AGAATTTTTGTTGCTGGCCA+TGG + chr4_2:60727721-60727740 None:intergenic 40.0%
GCAACCAATCTACAGGTATT+TGG + chr4_2:60726689-60726708 None:intergenic 40.0%
CTATCAAGCAACCAATCTAC+AGG + chr4_2:60726696-60726715 None:intergenic 40.0%
! GTAGATTGGTTGCTTGATAG+CGG - chr4_2:60726696-60726715 Msa0612290:three_prime_UTR 40.0%
AGTACTCAAAACAGTAGCCA+TGG - chr4_2:60726945-60726964 Msa0612290:three_prime_UTR 40.0%
GAGATCTTGGGTATCTATCT+AGG + chr4_2:60727097-60727116 None:intergenic 40.0%
!! CTGCTTGGTCTTTTTAGAGA+AGG - chr4_2:60727146-60727165 Msa0612290:intron 40.0%
ACAATGAGCTCTTCTTCTTC+AGG + chr4_2:60727465-60727484 None:intergenic 40.0%
ATTTGCTTTCCGTGTACATC+AGG - chr4_2:60727497-60727516 Msa0612290:intron 40.0%
CTCAGAATGGTCCTTTACAA+TGG + chr4_2:60727573-60727592 None:intergenic 40.0%
GACCATTCTGAGCAATGTAA+AGG - chr4_2:60727581-60727600 Msa0612290:intron 40.0%
GTCCTTTACATTGCTCAGAA+TGG + chr4_2:60727586-60727605 None:intergenic 40.0%
TCATGTCATGTTCAATGCCA+TGG - chr4_2:60727701-60727720 Msa0612290:intron 40.0%
! AGAATTTTTGTTGCTGGCCA+TGG + chr4_2:60727721-60727740 None:intergenic 40.0%
ACTGTATGCTTGTGTCTGCT+TGG - chr4_2:60727131-60727150 Msa0612290:intron 45.0%
TCTGCTGAGCTTGAAACTGT+TGG - chr4_2:60727432-60727451 Msa0612290:intron 45.0%
!! AGCTTGAAACTGTTGGTGGT+GGG - chr4_2:60727439-60727458 Msa0612290:intron 45.0%
GAAGAAGAGCTCATTGTCCA+AGG - chr4_2:60727468-60727487 Msa0612290:intron 45.0%
GGAAAGCAAATCTGACTCCT+TGG + chr4_2:60727488-60727507 None:intergenic 45.0%
!! GTTTGCTAGCGGTTTTGATG+CGG - chr4_2:60727637-60727656 Msa0612290:intron 45.0%
GCATTGAACATGACATGACC+TGG + chr4_2:60727699-60727718 None:intergenic 45.0%
TTGCTCCTGCATTAACATGC+TGG + chr4_2:60727755-60727774 None:intergenic 45.0%
ACTGTATGCTTGTGTCTGCT+TGG - chr4_2:60727131-60727150 Msa0612290:intron 45.0%
TCTGCTGAGCTTGAAACTGT+TGG - chr4_2:60727432-60727451 Msa0612290:intron 45.0%
!! AGCTTGAAACTGTTGGTGGT+GGG - chr4_2:60727439-60727458 Msa0612290:intron 45.0%
GAAGAAGAGCTCATTGTCCA+AGG - chr4_2:60727468-60727487 Msa0612290:intron 45.0%
GGAAAGCAAATCTGACTCCT+TGG + chr4_2:60727488-60727507 None:intergenic 45.0%
!! GTTTGCTAGCGGTTTTGATG+CGG - chr4_2:60727637-60727656 Msa0612290:intron 45.0%
GCATTGAACATGACATGACC+TGG + chr4_2:60727699-60727718 None:intergenic 45.0%
TTGCTCCTGCATTAACATGC+TGG + chr4_2:60727755-60727774 None:intergenic 45.0%
!! TGATAGCGGTTATGCCACCA+AGG - chr4_2:60726710-60726729 Msa0612290:three_prime_UTR 50.0%
GACAAGCTGCTGACAGCAAA+AGG - chr4_2:60726995-60727014 Msa0612290:CDS 50.0%
GCTGAGCTTGAAACTGTTGG+TGG - chr4_2:60727435-60727454 Msa0612290:intron 50.0%
! GAGCTTGAAACTGTTGGTGG+TGG - chr4_2:60727438-60727457 Msa0612290:intron 50.0%
CTCCTGCATTAACATGCTGG+AGG + chr4_2:60727752-60727771 None:intergenic 50.0%
CACAAGAACAAGCGCCAAAG+TGG + chr4_2:60727940-60727959 None:intergenic 50.0%
!! TGATAGCGGTTATGCCACCA+AGG - chr4_2:60726710-60726729 Msa0612290:three_prime_UTR 50.0%
GACAAGCTGCTGACAGCAAA+AGG - chr4_2:60726995-60727014 Msa0612290:CDS 50.0%
GCTGAGCTTGAAACTGTTGG+TGG - chr4_2:60727435-60727454 Msa0612290:intron 50.0%
! GAGCTTGAAACTGTTGGTGG+TGG - chr4_2:60727438-60727457 Msa0612290:intron 50.0%
CTCCTGCATTAACATGCTGG+AGG + chr4_2:60727752-60727771 None:intergenic 50.0%
CACAAGAACAAGCGCCAAAG+TGG + chr4_2:60727940-60727959 None:intergenic 50.0%
GGCCTCCAGCATGTTAATGC+AGG - chr4_2:60727747-60727766 Msa0612290:intron 55.0%
GGCCTCCAGCATGTTAATGC+AGG - chr4_2:60727747-60727766 Msa0612290:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 60726621 60728112 60726621 ID=Msa0612290;Name=Msa0612290
chr4_2 mRNA 60726621 60728112 60726621 ID=Msa0612290-mRNA-1;Parent=Msa0612290;Name=Msa0612290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=30|1|1|1|1|1|3|349|113
chr4_2 exon 60726621 60727095 60726621 ID=Msa0612290-mRNA-1:exon:16399;Parent=Msa0612290-mRNA-1
chr4_2 exon 60727215 60727349 60727215 ID=Msa0612290-mRNA-1:exon:16398;Parent=Msa0612290-mRNA-1
chr4_2 exon 60728002 60728112 60728002 ID=Msa0612290-mRNA-1:exon:16397;Parent=Msa0612290-mRNA-1
chr4_2 five_prime_UTR 60728083 60728112 60728083 ID=Msa0612290-mRNA-1:five_prime_utr;Parent=Msa0612290-mRNA-1
chr4_2 CDS 60728002 60728082 60728002 ID=Msa0612290-mRNA-1:cds;Parent=Msa0612290-mRNA-1
chr4_2 CDS 60727215 60727349 60727215 ID=Msa0612290-mRNA-1:cds;Parent=Msa0612290-mRNA-1
chr4_2 CDS 60726970 60727095 60726970 ID=Msa0612290-mRNA-1:cds;Parent=Msa0612290-mRNA-1
chr4_2 three_prime_UTR 60726621 60726969 60726621 ID=Msa0612290-mRNA-1:three_prime_utr;Parent=Msa0612290-mRNA-1
Gene Sequence

>Msa0612290

ATGAGAGAGTCAGCAACAAAGAGATTCAAAGTCTTCCAAATACCTGTAGATTGGTTGCTTGATAGCGGTTATGCCACCAAGATAAAGCTGGCATCTGTAAAATTGGCTATGAAGTATATGAAGAGAGTTTCTGCTGAGCTTGAAACTGTTGGTGGTGGGCCTGAAGAAGAAGAGCTCATTGTCCAAGGAGTCAGATTTGCTTTCCGTGTACATCAGTTTGCTAGCGGTTTTGATGCGGATACAATGAGAGCATTCCAAGAATTGAGAGATAAAGCCAGGTCATGTCATGTTCAATGCCATGGCCAGCAACAAAAATTCTTGTGCAGGCCTCCAGCATGTTAA
Protein sequence

>Msa0612290

MRESATKRFKVFQIPVDWLLDSGYATKIKLASVKLAMKYMKRVSAELETVGGGPEEEELIVQGVRFAFRVHQFASGFDADTMRAFQELRDKARSCHVQCHGQQQKFLCRPPAC*