AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0317340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317340 MtrunA17_Chr2g0290471 96.552 145 3 2 1 145 127 269 2.31e-92 268
Msa0317340 MtrunA17_Chr8g0386231 39.535 172 72 4 5 145 151 321 3.99e-28 105
Msa0317340 MtrunA17_Chr3g0128091 40.000 160 76 4 5 144 124 283 7.70e-25 96.3
Msa0317340 MtrunA17_Chr3g0132061 44.633 177 63 4 2 145 128 302 9.56e-25 96.3
Msa0317340 MtrunA17_Chr3g0132051 44.755 143 71 2 5 145 130 266 1.17e-21 87.4
Msa0317340 MtrunA17_Chr3g0094841 33.803 142 75 2 4 145 115 237 1.10e-17 76.6
Msa0317340 MtrunA17_Chr8g0337951 39.073 151 82 2 2 144 116 264 2.33e-16 73.2
Msa0317340 MtrunA17_Chr8g0343041 41.176 68 40 0 3 70 71 138 9.95e-14 66.6
Msa0317340 MtrunA17_Chr1g0180631 26.263 198 88 5 6 145 136 333 1.92e-12 63.2
Msa0317340 MtrunA17_Chr4g0028461 38.043 92 56 1 8 98 69 160 1.61e-11 60.5
Msa0317340 MtrunA17_Chr5g0429111 33.333 180 79 3 6 144 142 321 3.00e-11 59.7
Msa0317340 MtrunA17_Chr5g0429111 31.707 82 56 0 8 89 56 137 5.66e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317340 AT5G50740.5 42.236 161 74 4 4 145 123 283 8.42e-30 109
Msa0317340 AT5G50740.1 42.236 161 74 4 4 145 123 283 8.42e-30 109
Msa0317340 AT5G50740.3 42.236 161 74 4 4 145 130 290 9.25e-30 109
Msa0317340 AT5G63530.2 35.393 178 71 2 12 145 163 340 4.32e-21 87.4
Msa0317340 AT5G63530.1 35.393 178 71 2 12 145 178 355 5.29e-21 87.4
Msa0317340 AT3G02960.1 31.944 144 78 2 2 145 123 246 6.56e-19 80.1
Msa0317340 AT5G24580.5 29.050 179 85 3 4 144 57 231 2.57e-18 78.6
Msa0317340 AT5G24580.3 29.050 179 85 3 4 144 141 315 1.69e-17 77.4
Msa0317340 AT5G24580.2 29.050 179 85 3 4 144 143 317 1.86e-17 77.4
Msa0317340 AT5G24580.2 37.500 64 40 0 7 70 57 120 8.27e-11 58.9
Msa0317340 AT5G24580.4 29.050 179 85 3 4 144 144 318 2.19e-17 77.0
Msa0317340 AT5G24580.4 37.500 64 40 0 7 70 58 121 8.06e-11 58.9
Msa0317340 AT5G24580.1 29.050 179 85 3 4 144 144 318 2.19e-17 77.0
Msa0317340 AT5G24580.1 37.500 64 40 0 7 70 58 121 8.06e-11 58.9
Msa0317340 AT5G60800.1 33.758 157 86 3 3 145 131 283 2.75e-14 68.2
Msa0317340 AT5G60800.2 33.121 157 87 3 3 145 131 283 2.17e-13 65.9

Find 20 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAAAGAAGAGGAGAAAA+AGG 0.192651 2_4:-15015806 Msa0317340:CDS
AAACGTATCCGTAAGATTAA+AGG 0.281091 2_4:-15016606 Msa0317340:intron
AGAATCAGTGGAAACAGATT+TGG 0.303796 2_4:-15015947 Msa0317340:CDS
AATGATCAAGCCATAGTAAA+AGG 0.311623 2_4:-15015922 Msa0317340:CDS
ACTTCATCAACAAGCTTTGC+TGG 0.318902 2_4:+15015889 None:intergenic
AAATCAAACGTAGTGAATAT+TGG 0.336929 2_4:-15015708 Msa0317340:CDS
TACATAACAGAACAAGCATT+TGG 0.386302 2_4:+15015628 None:intergenic
TCTTTGTACCTTTAATCTTA+CGG 0.421997 2_4:+15016598 None:intergenic
GAATCAGTGGAAACAGATTT+GGG 0.429156 2_4:-15015946 Msa0317340:CDS
TGTGAAGGAGGAAGAAAAGA+AGG 0.465697 2_4:-15015836 Msa0317340:CDS
TTCTCATCACTGAAAATCTC+AGG 0.471345 2_4:+15015652 None:intergenic
GAAAGTGAAGAAGAAAACAA+AGG 0.495745 2_4:-15015754 Msa0317340:CDS
AGAACTATTGTTACCACAGG+TGG 0.515740 2_4:+15016672 None:intergenic
TTGAGAACTATTGTTACCAC+AGG 0.539096 2_4:+15016669 None:intergenic
GGAAGAAGAGAAGAAAGAAG+AGG 0.545102 2_4:-15015815 Msa0317340:CDS
GCAAGCTTCTATTGTGAAGG+AGG 0.578314 2_4:-15015848 Msa0317340:CDS
AAAGCAAGCTTCTATTGTGA+AGG 0.586936 2_4:-15015851 Msa0317340:CDS
TTGTTGATGAAGTGTTCAAG+AGG 0.592575 2_4:-15015879 Msa0317340:CDS
ACTATTGTTACCACAGGTGG+AGG 0.612633 2_4:+15016675 None:intergenic
ATATGCAGGAGTAGAATCAG+TGG 0.635889 2_4:-15015959 Msa0317340:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATGTGCTTTATCTAAA+AGG + chr2_4:15015946-15015965 None:intergenic 20.0%
!! TTATAAAGATAGGAGTTTAT+AGG + chr2_4:15016234-15016253 None:intergenic 20.0%
!! TCAAATTATGTTTATTTCAG+TGG + chr2_4:15016279-15016298 None:intergenic 20.0%
!! GTGTTAATAATAATATATGC+AGG - chr2_4:15016329-15016348 Msa0317340:intron 20.0%
!! ATTAATGTGCTTTATCTAAA+AGG + chr2_4:15015946-15015965 None:intergenic 20.0%
!! TTATAAAGATAGGAGTTTAT+AGG + chr2_4:15016234-15016253 None:intergenic 20.0%
!! TCAAATTATGTTTATTTCAG+TGG + chr2_4:15016279-15016298 None:intergenic 20.0%
!! GTGTTAATAATAATATATGC+AGG - chr2_4:15016329-15016348 Msa0317340:intron 20.0%
! TCTTTGTACCTTTAATCTTA+CGG + chr2_4:15015707-15015726 None:intergenic 25.0%
! AACGATCACATTATAAAGAT+AGG + chr2_4:15016244-15016263 None:intergenic 25.0%
! AAATCAAACGTAGTGAATAT+TGG - chr2_4:15016594-15016613 Msa0317340:intron 25.0%
! TCTTTGTACCTTTAATCTTA+CGG + chr2_4:15015707-15015726 None:intergenic 25.0%
! AACGATCACATTATAAAGAT+AGG + chr2_4:15016244-15016263 None:intergenic 25.0%
! AAATCAAACGTAGTGAATAT+TGG - chr2_4:15016594-15016613 Msa0317340:intron 25.0%
AAACGTATCCGTAAGATTAA+AGG - chr2_4:15015696-15015715 Msa0317340:CDS 30.0%
AATACCAACAAGATTGAAAG+AGG + chr2_4:15015736-15015755 None:intergenic 30.0%
TTACAGTGTCATGAAGAAAA+TGG - chr2_4:15015842-15015861 Msa0317340:CDS 30.0%
ATGTGCTTTATCTAAAAGGA+TGG + chr2_4:15015942-15015961 None:intergenic 30.0%
TAAGTTGTCTAATGATCTCA+TGG + chr2_4:15016041-15016060 None:intergenic 30.0%
AATGATCAAGCCATAGTAAA+AGG - chr2_4:15016380-15016399 Msa0317340:intron 30.0%
! ATCAATCACTCCTTTTACTA+TGG + chr2_4:15016393-15016412 None:intergenic 30.0%
AAAAAGGAAGAAGTCAAAGA+AGG - chr2_4:15016512-15016531 Msa0317340:intron 30.0%
GAAAGTGAAGAAGAAAACAA+AGG - chr2_4:15016548-15016567 Msa0317340:intron 30.0%
! TAGTCAACATAGTCTTTTGA+TGG + chr2_4:15016620-15016639 None:intergenic 30.0%
TACATAACAGAACAAGCATT+TGG + chr2_4:15016677-15016696 None:intergenic 30.0%
AAACGTATCCGTAAGATTAA+AGG - chr2_4:15015696-15015715 Msa0317340:CDS 30.0%
AATACCAACAAGATTGAAAG+AGG + chr2_4:15015736-15015755 None:intergenic 30.0%
TTACAGTGTCATGAAGAAAA+TGG - chr2_4:15015842-15015861 Msa0317340:CDS 30.0%
ATGTGCTTTATCTAAAAGGA+TGG + chr2_4:15015942-15015961 None:intergenic 30.0%
TAAGTTGTCTAATGATCTCA+TGG + chr2_4:15016041-15016060 None:intergenic 30.0%
AATGATCAAGCCATAGTAAA+AGG - chr2_4:15016380-15016399 Msa0317340:intron 30.0%
! ATCAATCACTCCTTTTACTA+TGG + chr2_4:15016393-15016412 None:intergenic 30.0%
AAAAAGGAAGAAGTCAAAGA+AGG - chr2_4:15016512-15016531 Msa0317340:intron 30.0%
GAAAGTGAAGAAGAAAACAA+AGG - chr2_4:15016548-15016567 Msa0317340:intron 30.0%
! TAGTCAACATAGTCTTTTGA+TGG + chr2_4:15016620-15016639 None:intergenic 30.0%
TACATAACAGAACAAGCATT+TGG + chr2_4:15016677-15016696 None:intergenic 30.0%
TTGAGAACTATTGTTACCAC+AGG + chr2_4:15015636-15015655 None:intergenic 35.0%
CAACAAGATTGAAAGAGGAA+AGG + chr2_4:15015731-15015750 None:intergenic 35.0%
CTTTCCTCTTTCAATCTTGT+TGG - chr2_4:15015729-15015748 Msa0317340:CDS 35.0%
!! CATCTTTGATTTGCATCGAA+CGG - chr2_4:15016066-15016085 Msa0317340:intron 35.0%
TGCAAGAAGTTAGATAGAAG+TGG + chr2_4:15016128-15016147 None:intergenic 35.0%
CTTCTATCTAACTTCTTGCA+TGG - chr2_4:15016128-15016147 Msa0317340:intron 35.0%
CAACTATCTAACTTCTTGCA+TGG - chr2_4:15016176-15016195 Msa0317340:intron 35.0%
AGAATCAGTGGAAACAGATT+TGG - chr2_4:15016355-15016374 Msa0317340:intron 35.0%
GAATCAGTGGAAACAGATTT+GGG - chr2_4:15016356-15016375 Msa0317340:intron 35.0%
! TTGTTGATGAAGTGTTCAAG+AGG - chr2_4:15016423-15016442 Msa0317340:intron 35.0%
AAAGCAAGCTTCTATTGTGA+AGG - chr2_4:15016451-15016470 Msa0317340:intron 35.0%
GAAGAAAGAAGAGGAGAAAA+AGG - chr2_4:15016496-15016515 Msa0317340:intron 35.0%
TTCTCATCACTGAAAATCTC+AGG + chr2_4:15016653-15016672 None:intergenic 35.0%
TTGAGAACTATTGTTACCAC+AGG + chr2_4:15015636-15015655 None:intergenic 35.0%
CAACAAGATTGAAAGAGGAA+AGG + chr2_4:15015731-15015750 None:intergenic 35.0%
CTTTCCTCTTTCAATCTTGT+TGG - chr2_4:15015729-15015748 Msa0317340:CDS 35.0%
!! CATCTTTGATTTGCATCGAA+CGG - chr2_4:15016066-15016085 Msa0317340:intron 35.0%
TGCAAGAAGTTAGATAGAAG+TGG + chr2_4:15016128-15016147 None:intergenic 35.0%
CTTCTATCTAACTTCTTGCA+TGG - chr2_4:15016128-15016147 Msa0317340:intron 35.0%
CAACTATCTAACTTCTTGCA+TGG - chr2_4:15016176-15016195 Msa0317340:intron 35.0%
AGAATCAGTGGAAACAGATT+TGG - chr2_4:15016355-15016374 Msa0317340:intron 35.0%
GAATCAGTGGAAACAGATTT+GGG - chr2_4:15016356-15016375 Msa0317340:intron 35.0%
! TTGTTGATGAAGTGTTCAAG+AGG - chr2_4:15016423-15016442 Msa0317340:intron 35.0%
AAAGCAAGCTTCTATTGTGA+AGG - chr2_4:15016451-15016470 Msa0317340:intron 35.0%
GAAGAAAGAAGAGGAGAAAA+AGG - chr2_4:15016496-15016515 Msa0317340:intron 35.0%
TTCTCATCACTGAAAATCTC+AGG + chr2_4:15016653-15016672 None:intergenic 35.0%
AGAACTATTGTTACCACAGG+TGG + chr2_4:15015633-15015652 None:intergenic 40.0%
TAGCGAATCCAGATTCACTA+AGG - chr2_4:15015976-15015995 Msa0317340:intron 40.0%
CTGTGTGCAACTACATAATG+TGG + chr2_4:15016005-15016024 None:intergenic 40.0%
! ACGGTTGAGATTGATAACCA+CGG - chr2_4:15016085-15016104 Msa0317340:intron 40.0%
ATATGCAGGAGTAGAATCAG+TGG - chr2_4:15016343-15016362 Msa0317340:intron 40.0%
ACTTCATCAACAAGCTTTGC+TGG + chr2_4:15016416-15016435 None:intergenic 40.0%
TGTGAAGGAGGAAGAAAAGA+AGG - chr2_4:15016466-15016485 Msa0317340:intron 40.0%
GGAAGAAGAGAAGAAAGAAG+AGG - chr2_4:15016487-15016506 Msa0317340:intron 40.0%
AGAACTATTGTTACCACAGG+TGG + chr2_4:15015633-15015652 None:intergenic 40.0%
TAGCGAATCCAGATTCACTA+AGG - chr2_4:15015976-15015995 Msa0317340:intron 40.0%
CTGTGTGCAACTACATAATG+TGG + chr2_4:15016005-15016024 None:intergenic 40.0%
! ACGGTTGAGATTGATAACCA+CGG - chr2_4:15016085-15016104 Msa0317340:intron 40.0%
ATATGCAGGAGTAGAATCAG+TGG - chr2_4:15016343-15016362 Msa0317340:intron 40.0%
ACTTCATCAACAAGCTTTGC+TGG + chr2_4:15016416-15016435 None:intergenic 40.0%
TGTGAAGGAGGAAGAAAAGA+AGG - chr2_4:15016466-15016485 Msa0317340:intron 40.0%
GGAAGAAGAGAAGAAAGAAG+AGG - chr2_4:15016487-15016506 Msa0317340:intron 40.0%
ACTATTGTTACCACAGGTGG+AGG + chr2_4:15015630-15015649 None:intergenic 45.0%
TACTGTCTCCTTACCACTTG+TGG - chr2_4:15015772-15015791 Msa0317340:CDS 45.0%
! TTTGTTGTGTGCACCACAAG+TGG + chr2_4:15015788-15015807 None:intergenic 45.0%
TGTGGTCACCTTAGTGAATC+TGG + chr2_4:15015987-15016006 None:intergenic 45.0%
GCAAGCTTCTATTGTGAAGG+AGG - chr2_4:15016454-15016473 Msa0317340:intron 45.0%
ACTATTGTTACCACAGGTGG+AGG + chr2_4:15015630-15015649 None:intergenic 45.0%
TACTGTCTCCTTACCACTTG+TGG - chr2_4:15015772-15015791 Msa0317340:CDS 45.0%
! TTTGTTGTGTGCACCACAAG+TGG + chr2_4:15015788-15015807 None:intergenic 45.0%
TGTGGTCACCTTAGTGAATC+TGG + chr2_4:15015987-15016006 None:intergenic 45.0%
GCAAGCTTCTATTGTGAAGG+AGG - chr2_4:15016454-15016473 Msa0317340:intron 45.0%
TGTGTGCACCACAAGTGGTA+AGG + chr2_4:15015783-15015802 None:intergenic 50.0%
TTAATGTGCTTGATGCGCCG+TGG + chr2_4:15016105-15016124 None:intergenic 50.0%
TGTGTGCACCACAAGTGGTA+AGG + chr2_4:15015783-15015802 None:intergenic 50.0%
TTAATGTGCTTGATGCGCCG+TGG + chr2_4:15016105-15016124 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 15015627 15016697 15015627 ID=Msa0317340;Name=Msa0317340
chr2_4 mRNA 15015627 15016697 15015627 ID=Msa0317340-mRNA-1;Parent=Msa0317340;Name=Msa0317340-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|145
chr2_4 exon 15016607 15016697 15016607 ID=Msa0317340-mRNA-1:exon:5640;Parent=Msa0317340-mRNA-1
chr2_4 exon 15015627 15015973 15015627 ID=Msa0317340-mRNA-1:exon:5639;Parent=Msa0317340-mRNA-1
chr2_4 CDS 15016607 15016697 15016607 ID=Msa0317340-mRNA-1:cds;Parent=Msa0317340-mRNA-1
chr2_4 CDS 15015627 15015973 15015627 ID=Msa0317340-mRNA-1:cds;Parent=Msa0317340-mRNA-1
Gene Sequence

>Msa0317340

CCTCCACCTGTGGTAACAATAGTTCTCAAAATCAGAATGCATTGTGATGCATGTGCTCAAGTTATACAGAAACGTATCCGTAAGATTAAAGGAGTAGAATCAGTGGAAACAGATTTGGGAAATGATCAAGCCATAGTAAAAGGAGTGATTGATCCAGCAAAGCTTGTTGATGAAGTGTTCAAGAGGACTAAAAAGCAAGCTTCTATTGTGAAGGAGGAAGAAAAGAAGGAAGAAGAGAAGAAAGAAGAGGAGAAAAAGGAAGAAGTCAAAGAAGGAGAAGAGAAAAAAGAAAGTGAAGAAGAAAACAAAGGAGAAGAAGATGATAATAAAACTGAAATCAAACGTAGTGAATATTGGCCATCAAAAGACTATGTTGACTATGTTTATGCACCTGAGATTTTCAGTGATGAGAATCCAAATGCTTGTTCTGTTATGTAG

Protein sequence

>Msa0317340

PPPVVTIVLKIRMHCDACAQVIQKRIRKIKGVESVETDLGNDQAIVKGVIDPAKLVDEVFKRTKKQASIVKEEEKKEEEKKEEEKKEEVKEGEEKKESEEENKGEEDDNKTEIKRSEYWPSKDYVDYVYAPEIFSDENPNACSVM*