AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0615610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615610 MtrunA17_Chr4g0052171 95.833 264 4 2 1 263 1 258 0.0 497
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAAGTGTAAATAATTT+TGG 0.110136 4_2:-65068583 None:intergenic
CTATCCACCTTTATAAATTA+TGG 0.151250 4_2:-65071491 None:intergenic
CAAATACACAACATGATAAT+AGG 0.198843 4_2:-65071837 None:intergenic
TATAAATTATGGAGAATAAT+TGG 0.215867 4_2:-65071480 None:intergenic
TTCAGCTTTCAATTCGAAAA+TGG 0.257771 4_2:-65068814 None:intergenic
AAGGAAGACCAATCTCTTCT+TGG 0.263332 4_2:-65071750 None:intergenic
AGAGAAAATTCACCGGAATT+AGG 0.272623 4_2:+65068663 Msa0615610:five_prime_UTR
AGTTACAACATTGGTGTATC+TGG 0.305118 4_2:+65071591 Msa0615610:three_prime_UTR
TCGATGGTTCTGTGCTTCTT+TGG 0.305984 4_2:-65068774 None:intergenic
AAATACACAACATGATAATA+GGG 0.309506 4_2:-65071836 None:intergenic
CCGTGTAAAATTGAGGAAAT+AGG 0.326875 4_2:+65068605 Msa0615610:five_prime_UTR
TTATTCTCCATAATTTATAA+AGG 0.337969 4_2:+65071484 Msa0615610:three_prime_UTR
CTGTCTGCGTAGTTTAATTT+CGG 0.343209 4_2:-65071341 None:intergenic
GTTGGGCTTGGTCGAGCTGC+TGG 0.357156 4_2:+65069678 Msa0615610:CDS
GCGCGTTGATATTCGGCGTC+AGG 0.359015 4_2:-65068885 None:intergenic
AGCCTATCTTCGGAAGAAAT+TGG 0.365885 4_2:+65071399 Msa0615610:CDS
TTGTATGATAAGTTAGTTAA+GGG 0.401547 4_2:+65069471 Msa0615610:CDS
AACTTGAAACCTGTTGGGCT+TGG 0.402705 4_2:+65069666 Msa0615610:CDS
TTTATTGGAATAGGGACCTT+TGG 0.405644 4_2:-65068925 None:intergenic
GTTGTATGATAAGTTAGTTA+AGG 0.408759 4_2:+65069470 Msa0615610:CDS
CTGAGCACAAGCGCAATGTT+AGG 0.424493 4_2:+65069715 Msa0615610:CDS
TGCCAATTTCTTCCGAAGAT+AGG 0.425699 4_2:-65071401 None:intergenic
TATGTACATGTATTTGAGAT+TGG 0.446518 4_2:+65071723 Msa0615610:three_prime_UTR
GCAGCTCGACCAAGCCCAAC+AGG 0.447516 4_2:-65069675 None:intergenic
TGTTTAACTTGAAACCTGTT+GGG 0.453678 4_2:+65069661 Msa0615610:CDS
GAATTAGGTTTAACGATGAC+GGG 0.453984 4_2:+65068678 Msa0615610:exon
AGAGTCGCGACGGAATCTTT+AGG 0.458235 4_2:+65068726 Msa0615610:CDS
CTGTTTAACTTGAAACCTGT+TGG 0.458401 4_2:+65069660 Msa0615610:CDS
GTCATCGTTAAACCTAATTC+CGG 0.465444 4_2:-65068675 None:intergenic
CGAAATTAAACTACGCAGAC+AGG 0.473262 4_2:+65071342 Msa0615610:CDS
AGCCAGTCGTGCGAGAGAAA+AGG 0.476028 4_2:+65071315 Msa0615610:CDS
GAGTTGAAAGCTATTAAAGA+TGG 0.477276 4_2:+65069411 Msa0615610:CDS
TTCTCCATAATTTATAAAGG+TGG 0.482341 4_2:+65071487 Msa0615610:three_prime_UTR
AACTTAAGCAAGCCTATCTT+CGG 0.486326 4_2:+65071389 Msa0615610:CDS
TTCTTGCAACACGTTGGGAT+TGG 0.494985 4_2:-65069580 None:intergenic
ACCGAGTTATTATTATTCAT+CGG 0.499078 4_2:+65071568 Msa0615610:three_prime_UTR
CGCGTTGATATTCGGCGTCA+GGG 0.499448 4_2:-65068884 None:intergenic
CTATGCTGCATTGGAGAAGA+AGG 0.501653 4_2:+65069443 Msa0615610:CDS
GCTGGTGATGGTGATGGTGA+TGG 0.502641 4_2:+65069618 Msa0615610:CDS
GAAAATGAACATGGTGGTGC+TGG 0.510141 4_2:+65069600 Msa0615610:CDS
AAGTCACTGAAATCAGTTGA+AGG 0.512826 4_2:-65071776 None:intergenic
GATGGTGATGGTGATGGTGA+TGG 0.515485 4_2:+65069624 Msa0615610:CDS
GGGAGAATTGTCTGATGAAG+AGG 0.516669 4_2:+65069491 Msa0615610:CDS
GTCAGGGCCTGATAATTCTC+TGG 0.517650 4_2:-65068868 None:intergenic
TTGGTAAACCAAGAAGAGAT+TGG 0.519620 4_2:+65071742 Msa0615610:three_prime_UTR
CTTCCATCCGTGTAAAATTG+AGG 0.520705 4_2:+65068598 Msa0615610:five_prime_UTR
ATCAGTGAGCTATGCTGCAT+TGG 0.523981 4_2:+65069434 Msa0615610:CDS
GACGGGGACGGAGGCACCGT+CGG 0.533538 4_2:+65068695 Msa0615610:CDS
GAAGAAATTGGCAGAACTGA+AGG 0.534198 4_2:+65071411 Msa0615610:CDS
TGAAATCAGTTGAAGGTCAA+AGG 0.545364 4_2:-65071769 None:intergenic
GCAGAACTGAAGGCCACTGT+TGG 0.546526 4_2:+65071421 Msa0615610:CDS
AGGTTTAACGATGACGGGGA+CGG 0.548885 4_2:+65068683 Msa0615610:exon
GGTGGTGCTGGTGATGGTGA+TGG 0.554003 4_2:+65069612 Msa0615610:CDS
GCGACTCTCTTCTTCTCCGA+CGG 0.562550 4_2:-65068711 None:intergenic
CGAGCTGCTGGAGTAGTTGA+CGG 0.566239 4_2:+65069690 Msa0615610:CDS
GAAGAAGAGAGAAAATTCAC+CGG 0.574648 4_2:+65068656 Msa0615610:five_prime_UTR
GGAATTAGGTTTAACGATGA+CGG 0.574948 4_2:+65068677 Msa0615610:exon
GAACATGGTGGTGCTGGTGA+TGG 0.586272 4_2:+65069606 Msa0615610:CDS
CACAGAACCATCGAGGGTGT+TGG 0.591588 4_2:+65068783 Msa0615610:CDS
GAAATAGGAACCGAGCGCAA+CGG 0.599113 4_2:+65068620 Msa0615610:five_prime_UTR
AATACACAACATGATAATAG+GGG 0.599341 4_2:-65071835 None:intergenic
CACAACATGATAATAGGGGA+TGG 0.622733 4_2:-65071831 None:intergenic
AGATGCACCAACACCCTCGA+TGG 0.631777 4_2:-65068790 None:intergenic
ATTCATCGGAGTTACAACAT+TGG 0.639519 4_2:+65071582 Msa0615610:three_prime_UTR
TCGCGACGGAATCTTTAGGA+TGG 0.640809 4_2:+65068730 Msa0615610:CDS
TCCGATGAATAATAATAACT+CGG 0.645707 4_2:-65071569 None:intergenic
CCACTGTTATCATGTTCGTG+TGG 0.646791 4_2:+65071803 Msa0615610:three_prime_UTR
GAGATCGCGTGCATCGACAC+CGG 0.655586 4_2:-65068952 None:intergenic
TTTAACGATGACGGGGACGG+AGG 0.656467 4_2:+65068686 Msa0615610:exon
GGAATCTTTAGGATGGCTAA+CGG 0.658502 4_2:+65068737 Msa0615610:CDS
AAGAAGCACAGAACCATCGA+GGG 0.672564 4_2:+65068777 Msa0615610:CDS
GGAGAAGAAGAGAGTCGCGA+CGG 0.681156 4_2:+65068716 Msa0615610:CDS
CCACACGAACATGATAACAG+TGG 0.692369 4_2:-65071803 None:intergenic
AAAGAAGCACAGAACCATCG+AGG 0.694787 4_2:+65068776 Msa0615610:CDS
AATTAGGTTTAACGATGACG+GGG 0.716816 4_2:+65068679 Msa0615610:exon
CTCAGATTCAGATCCAACAG+TGG 0.724935 4_2:-65071434 None:intergenic
GTGTTGCAAGAAAATGAACA+TGG 0.731258 4_2:+65069591 Msa0615610:CDS
TTGCAAGAAAATGAACATGG+TGG 0.793243 4_2:+65069594 Msa0615610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTATTTTAATTGTTC+AGG + chr4_2:65069020-65069039 Msa0615610:intron 10.0%
!! AAACATAAAGTGAAATAAAA+GGG - chr4_2:65068994-65069013 None:intergenic 15.0%
!! AAAAAAAAAAAACTCTTTCT+AGG + chr4_2:65071032-65071051 Msa0615610:intron 15.0%
!!! TTTTAAAAATTTTGATTTGC+AGG + chr4_2:65071278-65071297 Msa0615610:intron 15.0%
!!! TTTAAAAATTTTGATTTGCA+GGG + chr4_2:65071279-65071298 Msa0615610:intron 15.0%
!! TATAAATTATGGAGAATAAT+TGG - chr4_2:65071483-65071502 None:intergenic 15.0%
!! TTATTCTCCATAATTTATAA+AGG + chr4_2:65071484-65071503 Msa0615610:three_prime_UTR 15.0%
!!! ATGGAAGTGTAAATAATTTT+GGG - chr4_2:65068585-65068604 None:intergenic 20.0%
!! GAAACATAAAGTGAAATAAA+AGG - chr4_2:65068995-65069014 None:intergenic 20.0%
!!! TGATTTTGTAAATTTGATTC+TGG + chr4_2:65069102-65069121 Msa0615610:intron 20.0%
!! TTTGAATATAAATTTGAGTC+TGG + chr4_2:65069158-65069177 Msa0615610:intron 20.0%
!! TAAAGATATAAGTGTATGTT+TGG - chr4_2:65069300-65069319 None:intergenic 20.0%
!!! AATTTGTGTTTGTTGATAAT+TGG + chr4_2:65069335-65069354 Msa0615610:intron 20.0%
!!! ATTTGTGTTTGTTGATAATT+GGG + chr4_2:65069336-65069355 Msa0615610:intron 20.0%
!! TTGTATGATAAGTTAGTTAA+GGG + chr4_2:65069471-65069490 Msa0615610:CDS 20.0%
!!! CAATGTTTTTGTTCTATATA+TGG + chr4_2:65069766-65069785 Msa0615610:intron 20.0%
!!! TTTTTGTTCTATATATGGTT+AGG + chr4_2:65069771-65069790 Msa0615610:intron 20.0%
!!! TTCTTTGAAAACTTAACTTT+TGG + chr4_2:65069823-65069842 Msa0615610:intron 20.0%
!! ACCAAAATATGCTTTATATA+TGG + chr4_2:65069984-65070003 Msa0615610:intron 20.0%
!! CCAAAATATGCTTTATATAT+GGG + chr4_2:65069985-65070004 Msa0615610:intron 20.0%
!! AATAATCTATGTGAATACAA+TGG + chr4_2:65070430-65070449 Msa0615610:intron 20.0%
!! TAATGACTTACTATAAGTAA+CGG + chr4_2:65070981-65071000 Msa0615610:intron 20.0%
!!! TTTGTCTTTTACATTTAAGT+AGG + chr4_2:65071090-65071109 Msa0615610:intron 20.0%
!!! TTGTCTTTTACATTTAAGTA+GGG + chr4_2:65071091-65071110 Msa0615610:intron 20.0%
!! AAGTAACAAGTAACAATAAT+AGG - chr4_2:65071148-65071167 None:intergenic 20.0%
!!! TTTTTTATCCATGATTATTC+TGG + chr4_2:65071210-65071229 Msa0615610:intron 20.0%
!!! TTTTTATCCATGATTATTCT+GGG + chr4_2:65071211-65071230 Msa0615610:intron 20.0%
!!! GCAAATATTTCCTTTAAAAA+AGG + chr4_2:65071520-65071539 Msa0615610:three_prime_UTR 20.0%
!!! TTTCTTTTTTGATTATCTGA+GGG - chr4_2:65071637-65071656 None:intergenic 20.0%
!! TTATTTACAAGAGAAAAAAG+AGG - chr4_2:65071686-65071705 None:intergenic 20.0%
!! AAATACACAACATGATAATA+GGG - chr4_2:65071839-65071858 None:intergenic 20.0%
!!! GATGGAAGTGTAAATAATTT+TGG - chr4_2:65068586-65068605 None:intergenic 25.0%
! TACAGTGAGAAAAATTGATT+TGG + chr4_2:65069072-65069091 Msa0615610:intron 25.0%
! AGAATCAAAGCTCAAAATTT+AGG + chr4_2:65069192-65069211 Msa0615610:intron 25.0%
! CAACAAACACAAATTCAATT+AGG - chr4_2:65069330-65069349 None:intergenic 25.0%
!!! TGTGTTTGTTGTTATGATTA+GGG + chr4_2:65069380-65069399 Msa0615610:intron 25.0%
! GTTGTATGATAAGTTAGTTA+AGG + chr4_2:65069470-65069489 Msa0615610:CDS 25.0%
!!! CTTTTGGATGATATTGATTT+TGG + chr4_2:65069839-65069858 Msa0615610:intron 25.0%
! CTTTGTATGAAATGTGATTT+CGG + chr4_2:65069911-65069930 Msa0615610:intron 25.0%
!! CCCATATATAAAGCATATTT+TGG - chr4_2:65069988-65070007 None:intergenic 25.0%
! TACGTATTACAAGTAAAACT+TGG + chr4_2:65070143-65070162 Msa0615610:intron 25.0%
!!! TCTTCTAGAGCTTTTAATTT+TGG + chr4_2:65070840-65070859 Msa0615610:intron 25.0%
!!! CTTTTAATTTTGGCTTTAGA+GGG + chr4_2:65070850-65070869 Msa0615610:intron 25.0%
!!! TTTTAATTTTGGCTTTAGAG+GGG + chr4_2:65070851-65070870 Msa0615610:intron 25.0%
!! AAAATTTGACTTAATCCAGT+AGG - chr4_2:65070891-65070910 None:intergenic 25.0%
!! ACTGGATTAAGTCAAATTTT+TGG + chr4_2:65070891-65070910 Msa0615610:intron 25.0%
! CTTAAATGTAAAAGACAAAC+AGG - chr4_2:65071089-65071108 None:intergenic 25.0%
!!! TGTTACTTGTTACTTTTTTC+CGG + chr4_2:65071153-65071172 Msa0615610:intron 25.0%
! TTCTCCATAATTTATAAAGG+TGG + chr4_2:65071487-65071506 Msa0615610:three_prime_UTR 25.0%
! CTATCCACCTTTATAAATTA+TGG - chr4_2:65071494-65071513 None:intergenic 25.0%
! ACCGAGTTATTATTATTCAT+CGG + chr4_2:65071568-65071587 Msa0615610:three_prime_UTR 25.0%
! TCCGATGAATAATAATAACT+CGG - chr4_2:65071572-65071591 None:intergenic 25.0%
!!! CTTTCTTTTTTGATTATCTG+AGG - chr4_2:65071638-65071657 None:intergenic 25.0%
!! TATGTACATGTATTTGAGAT+TGG + chr4_2:65071723-65071742 Msa0615610:three_prime_UTR 25.0%
! AATACACAACATGATAATAG+GGG - chr4_2:65071838-65071857 None:intergenic 25.0%
! CAAATACACAACATGATAAT+AGG - chr4_2:65071840-65071859 None:intergenic 25.0%
! CCTATTTCCTCAATTTTACA+CGG - chr4_2:65068608-65068627 None:intergenic 30.0%
TTCAGCTTTCAATTCGAAAA+TGG - chr4_2:65068817-65068836 None:intergenic 30.0%
! TGATAATTCTCTGGACTTTT+TGG - chr4_2:65068862-65068881 None:intergenic 30.0%
AAAAAGTCCAGAGAATTATC+AGG + chr4_2:65068861-65068880 Msa0615610:CDS 30.0%
!! TTTGGAAGTGATGATGTTTT+TGG - chr4_2:65068910-65068929 None:intergenic 30.0%
AAAAACATCATCACTTCCAA+AGG + chr4_2:65068909-65068928 Msa0615610:CDS 30.0%
AAGTGAAATAAAAGGGAAAC+AGG - chr4_2:65068987-65069006 None:intergenic 30.0%
TTTACTTTCAACTCAGGTTT+CGG - chr4_2:65069128-65069147 None:intergenic 30.0%
AATCACTTTACTTTCAACTC+AGG - chr4_2:65069134-65069153 None:intergenic 30.0%
!! GTGTGTTTGTTGTTATGATT+AGG + chr4_2:65069379-65069398 Msa0615610:intron 30.0%
GAGTTGAAAGCTATTAAAGA+TGG + chr4_2:65069411-65069430 Msa0615610:CDS 30.0%
!! TGTTTAACTTGAAACCTGTT+GGG + chr4_2:65069661-65069680 Msa0615610:CDS 30.0%
GTTCTATATATGGTTAGGAT+AGG + chr4_2:65069776-65069795 Msa0615610:intron 30.0%
TAAGAAAATGTTTGAGAGAG+TGG + chr4_2:65070191-65070210 Msa0615610:intron 30.0%
! ATGATGATGATCAAGATTAC+AGG + chr4_2:65070246-65070265 Msa0615610:intron 30.0%
! TGATGATGATCAAGATTACA+GGG + chr4_2:65070247-65070266 Msa0615610:intron 30.0%
TGTTTATCATATCCCATTTG+TGG - chr4_2:65070632-65070651 None:intergenic 30.0%
TTAACTACTTGTGCTTAGTT+TGG + chr4_2:65070695-65070714 Msa0615610:intron 30.0%
TAACTACTTGTGCTTAGTTT+GGG + chr4_2:65070696-65070715 Msa0615610:intron 30.0%
! TTAGTTTGGGAGTGTAAAAT+AGG + chr4_2:65070709-65070728 Msa0615610:intron 30.0%
! TAGTTTGGGAGTGTAAAATA+GGG + chr4_2:65070710-65070729 Msa0615610:intron 30.0%
AGTATCCTTCTTGTTAGAAT+TGG + chr4_2:65070767-65070786 Msa0615610:intron 30.0%
!!! GCTTTTAATTTTGGCTTTAG+AGG + chr4_2:65070849-65070868 Msa0615610:intron 30.0%
!!! GTTACTTGTTACTTTTTTCC+GGG + chr4_2:65071154-65071173 Msa0615610:intron 30.0%
!!! TTACTTGTTACTTTTTTCCG+GGG + chr4_2:65071155-65071174 Msa0615610:intron 30.0%
! TTTCCTTTAAAAAAGGCATG+AGG + chr4_2:65071527-65071546 Msa0615610:three_prime_UTR 30.0%
! TTTCCTCAATTTTACACGGA+TGG - chr4_2:65068604-65068623 None:intergenic 35.0%
CCGTGTAAAATTGAGGAAAT+AGG + chr4_2:65068605-65068624 Msa0615610:five_prime_UTR 35.0%
GAAGAAGAGAGAAAATTCAC+CGG + chr4_2:65068656-65068675 Msa0615610:five_prime_UTR 35.0%
AGAGAAAATTCACCGGAATT+AGG + chr4_2:65068663-65068682 Msa0615610:five_prime_UTR 35.0%
GTCATCGTTAAACCTAATTC+CGG - chr4_2:65068678-65068697 None:intergenic 35.0%
GGAATTAGGTTTAACGATGA+CGG + chr4_2:65068677-65068696 Msa0615610:exon 35.0%
GAATTAGGTTTAACGATGAC+GGG + chr4_2:65068678-65068697 Msa0615610:exon 35.0%
AATTAGGTTTAACGATGACG+GGG + chr4_2:65068679-65068698 Msa0615610:exon 35.0%
TTTATTGGAATAGGGACCTT+TGG - chr4_2:65068928-65068947 None:intergenic 35.0%
! CCGGAGTTTTTATTGGAATA+GGG - chr4_2:65068936-65068955 None:intergenic 35.0%
CCCTATTCCAATAAAAACTC+CGG + chr4_2:65068933-65068952 Msa0615610:CDS 35.0%
! ACCGGAGTTTTTATTGGAAT+AGG - chr4_2:65068937-65068956 None:intergenic 35.0%
! AAGCTCAAAATTTAGGTTCG+AGG + chr4_2:65069199-65069218 Msa0615610:intron 35.0%
GTTCGAGGTAGAATAGATTA+TGG + chr4_2:65069214-65069233 Msa0615610:intron 35.0%
!! ACTTTCGGAGCAATTTTAAC+AGG - chr4_2:65069273-65069292 None:intergenic 35.0%
TGTTAAAATTGCTCCGAAAG+TGG + chr4_2:65069272-65069291 Msa0615610:intron 35.0%
! TGTATGTTTGGTTCCACTTT+CGG - chr4_2:65069288-65069307 None:intergenic 35.0%
! TCATTTTCTTGCAACACGTT+GGG - chr4_2:65069588-65069607 None:intergenic 35.0%
! TTCATTTTCTTGCAACACGT+TGG - chr4_2:65069589-65069608 None:intergenic 35.0%
GTGTTGCAAGAAAATGAACA+TGG + chr4_2:65069591-65069610 Msa0615610:CDS 35.0%
TTGCAAGAAAATGAACATGG+TGG + chr4_2:65069594-65069613 Msa0615610:CDS 35.0%
!! CTGTTTAACTTGAAACCTGT+TGG + chr4_2:65069660-65069679 Msa0615610:CDS 35.0%
ATAAAACACACACTCCTTCA+AGG - chr4_2:65069881-65069900 None:intergenic 35.0%
!! AGGAGTGTGTGTTTTATGTT+TGG + chr4_2:65069884-65069903 Msa0615610:intron 35.0%
TGTATGAAATGTGATTTCGG+AGG + chr4_2:65069914-65069933 Msa0615610:intron 35.0%
!! AGTGATAGCAATGTTTTGAG+AGG + chr4_2:65070008-65070027 Msa0615610:intron 35.0%
TTCTAACCTTGAAGCTTGAA+AGG - chr4_2:65070037-65070056 None:intergenic 35.0%
! GAAGTGTCAAAATTTCTGTG+AGG + chr4_2:65070054-65070073 Msa0615610:intron 35.0%
ATATAGAAGTCCAGTGAGAT+TGG + chr4_2:65070288-65070307 Msa0615610:intron 35.0%
! TTTGCTACTTCTGTTAACCT+AGG - chr4_2:65070372-65070391 None:intergenic 35.0%
TGTAAAATAGGGCTGAGTTA+AGG + chr4_2:65070721-65070740 Msa0615610:intron 35.0%
ATGTGCCAATTCTAACAAGA+AGG - chr4_2:65070775-65070794 None:intergenic 35.0%
TCTTGTTAGAATTGGCACAT+AGG + chr4_2:65070775-65070794 Msa0615610:intron 35.0%
! CTCTAGAAGAGTGAATAAAC+GGG - chr4_2:65070830-65070849 None:intergenic 35.0%
!! GCTCTAGAAGAGTGAATAAA+CGG - chr4_2:65070831-65070850 None:intergenic 35.0%
TATAGTGCTGTAACCTATTG+AGG + chr4_2:65070954-65070973 Msa0615610:intron 35.0%
AAGTCATTACCTACCTCAAT+AGG - chr4_2:65070970-65070989 None:intergenic 35.0%
TATAAGTAACGGCTCTTTCT+AGG + chr4_2:65070992-65071011 Msa0615610:intron 35.0%
TAAGTAGGGAATGATGAAGA+TGG + chr4_2:65071105-65071124 Msa0615610:intron 35.0%
AAGTAGGGAATGATGAAGAT+GGG + chr4_2:65071106-65071125 Msa0615610:intron 35.0%
!!! TACTTGTTACTTTTTTCCGG+GGG + chr4_2:65071156-65071175 Msa0615610:intron 35.0%
GCAATTACCCAGAATAATCA+TGG - chr4_2:65071221-65071240 None:intergenic 35.0%
TCGTTAAATTTGTGAGTCTG+TGG + chr4_2:65071242-65071261 Msa0615610:intron 35.0%
! CTGTCTGCGTAGTTTAATTT+CGG - chr4_2:65071344-65071363 None:intergenic 35.0%
AACTTAAGCAAGCCTATCTT+CGG + chr4_2:65071389-65071408 Msa0615610:CDS 35.0%
!!! ACTCCTCATGCCTTTTTTAA+AGG - chr4_2:65071533-65071552 None:intergenic 35.0%
ATTCATCGGAGTTACAACAT+TGG + chr4_2:65071582-65071601 Msa0615610:three_prime_UTR 35.0%
AGTTACAACATTGGTGTATC+TGG + chr4_2:65071591-65071610 Msa0615610:three_prime_UTR 35.0%
TTGGTAAACCAAGAAGAGAT+TGG + chr4_2:65071742-65071761 Msa0615610:three_prime_UTR 35.0%
TGAAATCAGTTGAAGGTCAA+AGG - chr4_2:65071772-65071791 None:intergenic 35.0%
AAGTCACTGAAATCAGTTGA+AGG - chr4_2:65071779-65071798 None:intergenic 35.0%
CTTCCATCCGTGTAAAATTG+AGG + chr4_2:65068598-65068617 Msa0615610:five_prime_UTR 40.0%
!! GGAATCTTTAGGATGGCTAA+CGG + chr4_2:65068737-65068756 Msa0615610:CDS 40.0%
!!! GTTTTTGGCGCGTTGATATT+CGG - chr4_2:65068895-65068914 None:intergenic 40.0%
! ATCGACACCGGAGTTTTTAT+TGG - chr4_2:65068943-65068962 None:intergenic 40.0%
CGAGGTAGAATAGATTATGG+AGG + chr4_2:65069217-65069236 Msa0615610:intron 40.0%
!! CGGAGCAATTTTAACAGGTT+TGG - chr4_2:65069268-65069287 None:intergenic 40.0%
TCGGAGGAATTAATGTCGTT+TGG + chr4_2:65069930-65069949 Msa0615610:intron 40.0%
AGGTTTCCTTTCAAGCTTCA+AGG + chr4_2:65070028-65070047 Msa0615610:intron 40.0%
ATGCGGAAGCTAAATCTGTT+TGG + chr4_2:65070090-65070109 Msa0615610:intron 40.0%
! TAAAACTTGGTGACAGAGTG+AGG + chr4_2:65070156-65070175 Msa0615610:intron 40.0%
! AAAACTTGGTGACAGAGTGA+GGG + chr4_2:65070157-65070176 Msa0615610:intron 40.0%
ATACCAGTTGCAAGAATCCT+AGG + chr4_2:65070352-65070371 Msa0615610:intron 40.0%
TAACCTAGGATTCTTGCAAC+TGG - chr4_2:65070358-65070377 None:intergenic 40.0%
AATACCCACCTCGTAATTCA+AGG + chr4_2:65070504-65070523 Msa0615610:intron 40.0%
!!! AGTGTTGGACTTTTTTGCCA+TGG - chr4_2:65070538-65070557 None:intergenic 40.0%
GCAAAAAAGTCCAACACTTC+CGG + chr4_2:65070540-65070559 Msa0615610:intron 40.0%
CAAAAAAGTCCAACACTTCC+GGG + chr4_2:65070541-65070560 Msa0615610:intron 40.0%
GCTACACATAGAGAAAATCC+CGG - chr4_2:65070562-65070581 None:intergenic 40.0%
TATCCCATTTGTGGATCAAC+TGG - chr4_2:65070623-65070642 None:intergenic 40.0%
AGTAGGGAATGATGAAGATG+GGG + chr4_2:65071107-65071126 Msa0615610:intron 40.0%
TTGTGAGTCTGTGGCTAATT+TGG + chr4_2:65071251-65071270 Msa0615610:intron 40.0%
CGAAATTAAACTACGCAGAC+AGG + chr4_2:65071342-65071361 Msa0615610:CDS 40.0%
AGCCTATCTTCGGAAGAAAT+TGG + chr4_2:65071399-65071418 Msa0615610:CDS 40.0%
TGCCAATTTCTTCCGAAGAT+AGG - chr4_2:65071404-65071423 None:intergenic 40.0%
GAAGAAATTGGCAGAACTGA+AGG + chr4_2:65071411-65071430 Msa0615610:CDS 40.0%
AAGGAAGACCAATCTCTTCT+TGG - chr4_2:65071753-65071772 None:intergenic 40.0%
CACAACATGATAATAGGGGA+TGG - chr4_2:65071834-65071853 None:intergenic 40.0%
! TTTTGTTCTTCCGTTGCGCT+CGG - chr4_2:65068633-65068652 None:intergenic 45.0%
! TCGATGGTTCTGTGCTTCTT+TGG - chr4_2:65068777-65068796 None:intergenic 45.0%
! AAAGAAGCACAGAACCATCG+AGG + chr4_2:65068776-65068795 Msa0615610:CDS 45.0%
! AAGAAGCACAGAACCATCGA+GGG + chr4_2:65068777-65068796 Msa0615610:CDS 45.0%
ATCAGTGAGCTATGCTGCAT+TGG + chr4_2:65069434-65069453 Msa0615610:CDS 45.0%
CTATGCTGCATTGGAGAAGA+AGG + chr4_2:65069443-65069462 Msa0615610:CDS 45.0%
GGGAGAATTGTCTGATGAAG+AGG + chr4_2:65069491-65069510 Msa0615610:CDS 45.0%
TTCTTGCAACACGTTGGGAT+TGG - chr4_2:65069583-65069602 None:intergenic 45.0%
! GAAAATGAACATGGTGGTGC+TGG + chr4_2:65069600-65069619 Msa0615610:CDS 45.0%
AACTTGAAACCTGTTGGGCT+TGG + chr4_2:65069666-65069685 Msa0615610:CDS 45.0%
! AAAGTGCTTGTGAGCCTTGA+AGG + chr4_2:65069864-65069883 Msa0615610:intron 45.0%
! AAACTTGGTGACAGAGTGAG+GGG + chr4_2:65070158-65070177 Msa0615610:intron 45.0%
TCCACCTTGAATTACGAGGT+GGG - chr4_2:65070511-65070530 None:intergenic 45.0%
TGTGTCCACCTTGAATTACG+AGG - chr4_2:65070515-65070534 None:intergenic 45.0%
AATTCAAGGTGGACACACCA+TGG + chr4_2:65070518-65070537 Msa0615610:intron 45.0%
! AGAGAAAATCCCGGAAGTGT+TGG - chr4_2:65070553-65070572 None:intergenic 45.0%
ATGTGTAGCAAGTCTCTGAG+TGG + chr4_2:65070573-65070592 Msa0615610:intron 45.0%
AGCAAGTCTCTGAGTGGATA+AGG + chr4_2:65070579-65070598 Msa0615610:intron 45.0%
TCAGCCAGTTGATCCACAAA+TGG + chr4_2:65070616-65070635 Msa0615610:intron 45.0%
CAGCCAGTTGATCCACAAAT+GGG + chr4_2:65070617-65070636 Msa0615610:intron 45.0%
GTCAGTTGTTGAATGCCTAC+TGG + chr4_2:65070873-65070892 Msa0615610:intron 45.0%
GTGCTGTAACCTATTGAGGT+AGG + chr4_2:65070958-65070977 Msa0615610:intron 45.0%
!!! TTGTTACTTTTTTCCGGGGG+TGG + chr4_2:65071159-65071178 Msa0615610:intron 45.0%
!!! TGTTACTTTTTTCCGGGGGT+GGG + chr4_2:65071160-65071179 Msa0615610:intron 45.0%
CTCAGATTCAGATCCAACAG+TGG - chr4_2:65071437-65071456 None:intergenic 45.0%
CCACACGAACATGATAACAG+TGG - chr4_2:65071806-65071825 None:intergenic 45.0%
CCACTGTTATCATGTTCGTG+TGG + chr4_2:65071803-65071822 Msa0615610:three_prime_UTR 45.0%
GAAATAGGAACCGAGCGCAA+CGG + chr4_2:65068620-65068639 Msa0615610:five_prime_UTR 50.0%
!! AGGTTTAACGATGACGGGGA+CGG + chr4_2:65068683-65068702 Msa0615610:exon 50.0%
AGAGTCGCGACGGAATCTTT+AGG + chr4_2:65068726-65068745 Msa0615610:CDS 50.0%
! TCGCGACGGAATCTTTAGGA+TGG + chr4_2:65068730-65068749 Msa0615610:CDS 50.0%
GTCAGGGCCTGATAATTCTC+TGG - chr4_2:65068871-65068890 None:intergenic 50.0%
!! GATGGTGATGGTGATGGTGA+TGG + chr4_2:65069624-65069643 Msa0615610:CDS 50.0%
CTGAGCACAAGCGCAATGTT+AGG + chr4_2:65069715-65069734 Msa0615610:CDS 50.0%
GAGGAGCTTGTGCTTCAATG+CGG + chr4_2:65070073-65070092 Msa0615610:intron 50.0%
ACCCACCTCGTAATTCAAGG+TGG + chr4_2:65070507-65070526 Msa0615610:intron 50.0%
GTCCACCTTGAATTACGAGG+TGG - chr4_2:65070512-65070531 None:intergenic 50.0%
GGAATGATGAAGATGGGGCT+GGG + chr4_2:65071112-65071131 Msa0615610:intron 50.0%
!! TTTAACGATGACGGGGACGG+AGG + chr4_2:65068686-65068705 Msa0615610:exon 55.0%
GCGACTCTCTTCTTCTCCGA+CGG - chr4_2:65068714-65068733 None:intergenic 55.0%
GGAGAAGAAGAGAGTCGCGA+CGG + chr4_2:65068716-65068735 Msa0615610:CDS 55.0%
! CACAGAACCATCGAGGGTGT+TGG + chr4_2:65068783-65068802 Msa0615610:CDS 55.0%
AGATGCACCAACACCCTCGA+TGG - chr4_2:65068793-65068812 None:intergenic 55.0%
! CGCGTTGATATTCGGCGTCA+GGG - chr4_2:65068887-65068906 None:intergenic 55.0%
!! GAACATGGTGGTGCTGGTGA+TGG + chr4_2:65069606-65069625 Msa0615610:CDS 55.0%
!! GCTGGTGATGGTGATGGTGA+TGG + chr4_2:65069618-65069637 Msa0615610:CDS 55.0%
CGAGCTGCTGGAGTAGTTGA+CGG + chr4_2:65069690-65069709 Msa0615610:CDS 55.0%
GAAGCAGCGACCAATCTCAC+TGG - chr4_2:65070301-65070320 None:intergenic 55.0%
GGGAATGATGAAGATGGGGC+TGG + chr4_2:65071111-65071130 Msa0615610:intron 55.0%
! GGGTGGGAGCTTGAGGTTTT+TGG + chr4_2:65071176-65071195 Msa0615610:intron 55.0%
AGCCAGTCGTGCGAGAGAAA+AGG + chr4_2:65071315-65071334 Msa0615610:CDS 55.0%
! AGCCTTTTCTCTCGCACGAC+TGG - chr4_2:65071320-65071339 None:intergenic 55.0%
GCAGAACTGAAGGCCACTGT+TGG + chr4_2:65071421-65071440 Msa0615610:CDS 55.0%
! GCGCGTTGATATTCGGCGTC+AGG - chr4_2:65068888-65068907 None:intergenic 60.0%
! GAGATCGCGTGCATCGACAC+CGG - chr4_2:65068955-65068974 None:intergenic 60.0%
!! GGTGGTGCTGGTGATGGTGA+TGG + chr4_2:65069612-65069631 Msa0615610:CDS 60.0%
AAACCTCAAGCTCCCACCCC+CGG - chr4_2:65071175-65071194 None:intergenic 60.0%
GCAGCTCGACCAAGCCCAAC+AGG - chr4_2:65069678-65069697 None:intergenic 65.0%
! GTTGGGCTTGGTCGAGCTGC+TGG + chr4_2:65069678-65069697 Msa0615610:CDS 65.0%
TTTCCGGGGGTGGGAGCTTG+AGG + chr4_2:65071169-65071188 Msa0615610:intron 65.0%
!! GACGGGGACGGAGGCACCGT+CGG + chr4_2:65068695-65068714 Msa0615610:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 65068580 65071923 65068580 ID=Msa0615610;Name=Msa0615610
chr4_2 mRNA 65068580 65071923 65068580 ID=Msa0615610-mRNA-1;Parent=Msa0615610;Name=Msa0615610-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113|1|1|1|1|1|3|452|263
chr4_2 exon 65068580 65068976 65068580 ID=Msa0615610-mRNA-1:exon:18318;Parent=Msa0615610-mRNA-1
chr4_2 exon 65069401 65069736 65069401 ID=Msa0615610-mRNA-1:exon:18319;Parent=Msa0615610-mRNA-1
chr4_2 exon 65071300 65071923 65071300 ID=Msa0615610-mRNA-1:exon:18320;Parent=Msa0615610-mRNA-1
chr4_2 five_prime_UTR 65068580 65068692 65068580 ID=Msa0615610-mRNA-1:five_prime_utr;Parent=Msa0615610-mRNA-1
chr4_2 CDS 65068693 65068976 65068693 ID=Msa0615610-mRNA-1:cds;Parent=Msa0615610-mRNA-1
chr4_2 CDS 65069401 65069736 65069401 ID=Msa0615610-mRNA-1:cds;Parent=Msa0615610-mRNA-1
chr4_2 CDS 65071300 65071471 65071300 ID=Msa0615610-mRNA-1:cds;Parent=Msa0615610-mRNA-1
chr4_2 three_prime_UTR 65071472 65071923 65071472 ID=Msa0615610-mRNA-1:three_prime_utr;Parent=Msa0615610-mRNA-1
Gene Sequence

>Msa0615610

ATGACGGGGACGGAGGCACCGTCGGAGAAGAAGAGAGTCGCGACGGAATCTTTAGGATGGCTAACGGAATCGACGATAATGCCAAAGAAGCACAGAACCATCGAGGGTGTTGGTGCATCTTCCATTTTCGAATTGAAAGCTGAACTCTACAAATCTCAAGAAGATTCCAAAAAGTCCAGAGAATTATCAGGCCCTGACGCCGAATATCAACGCGCCAAAAACATCATCACTTCCAAAGGTCCCTATTCCAATAAAAACTCCGGTGTCGATGCACGCGATCTCAAGGATAAACTTGAGTTGAAAGCTATTAAAGATGGATCAGTGAGCTATGCTGCATTGGAGAAGAAGGCTGAGTTGTATGATAAGTTAGTTAAGGGAGAATTGTCTGATGAAGAGGATAAAGAGAAGTATTGTGTTGATTTTGTTCGTAAGAGCGATGATCATGATGATGATAATGTCTCTACCAATCCCAACGTGTTGCAAGAAAATGAACATGGTGGTGCTGGTGATGGTGATGGTGATGGTGATGGTGATGCTTTTTCGCTGTTTAACTTGAAACCTGTTGGGCTTGGTCGAGCTGCTGGAGTAGTTGACGGTGCTGAGCACAAGCGCAATGTTAGGGAAGTTCATGAAGAAGCCAGTCGTGCGAGAGAAAAGGCTTCCGAAATTAAACTACGCAGACAGGAGCAGTTAGCTGCTCAACGCGAGAAACTTAAGCAAGCCTATCTTCGGAAGAAATTGGCAGAACTGAAGGCCACTGTTGGATCTGAATCTGAGATTAAGCACACATGA
Protein sequence

>Msa0615610

MTGTEAPSEKKRVATESLGWLTESTIMPKKHRTIEGVGASSIFELKAELYKSQEDSKKSRELSGPDAEYQRAKNIITSKGPYSNKNSGVDARDLKDKLELKAIKDGSVSYAALEKKAELYDKLVKGELSDEEDKEKYCVDFVRKSDDHDDDNVSTNPNVLQENEHGGAGDGDGDGDGDAFSLFNLKPVGLGRAAGVVDGAEHKRNVREVHEEASRAREKASEIKLRRQEQLAAQREKLKQAYLRKKLAELKATVGSESEIKHT*