AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0654970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654970 MtrunA17_Chr4g0052171 96.183 262 5 2 1 261 1 258 0.0 497
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCCACCTTTATAAATTA+TGG 0.151250 4_3:+63361568 None:intergenic
CAAATACACATGATGATAAT+AGG 0.207901 4_3:+63361204 None:intergenic
CTATGTACATGAATTATTAT+AGG 0.249398 4_3:-63361337 Msa0654970:three_prime_UTR
TTCAGCTTTCAATTCGAAAA+TGG 0.257771 4_3:+63364256 None:intergenic
AAGGAAGACCAATCTCTTCT+TGG 0.263332 4_3:+63361291 None:intergenic
AGAGAAAATTCACCGGAATT+AGG 0.272623 4_3:-63364407 Msa0654970:five_prime_UTR
AGTTACAACATTGGTGTATC+TGG 0.305118 4_3:-63361468 Msa0654970:three_prime_UTR
TCGATGGTTCTGTGCTTCTT+TGG 0.305984 4_3:+63364296 None:intergenic
AAATACACATGATGATAATA+GGG 0.318564 4_3:+63361205 None:intergenic
CCGTGTAAAATTGAGGAAAT+AGG 0.326875 4_3:-63364465 None:intergenic
TTATTCTCCATAATTTATAA+AGG 0.337969 4_3:-63361575 Msa0654970:three_prime_UTR
CTGTCTGCGTAGTTTAATTT+CGG 0.343209 4_3:+63361718 None:intergenic
GTTGGGCTTGGTCGAGCTGC+TGG 0.357156 4_3:-63363377 Msa0654970:CDS
GCGCGTTGATATTCGGCGTC+AGG 0.359015 4_3:+63364185 None:intergenic
AGACCAATCTCTTCTTGGTT+TGG 0.363300 4_3:+63361296 None:intergenic
AGCCTATCTTCGGAAGAAAT+TGG 0.365885 4_3:-63361660 Msa0654970:CDS
TTGTATGATAAGTTAGTTAA+GGG 0.401547 4_3:-63363578 Msa0654970:CDS
AACTTGAAACCTGTTGGGCT+TGG 0.402705 4_3:-63363389 Msa0654970:CDS
TTTATTGGAATAGGGACCTT+TGG 0.405644 4_3:+63364145 None:intergenic
GTTGTATGATAAGTTAGTTA+AGG 0.408759 4_3:-63363579 Msa0654970:CDS
CTGAGCACAAGCGCAATGTT+AGG 0.424493 4_3:-63363340 Msa0654970:intron
TGCCAATTTCTTCCGAAGAT+AGG 0.425699 4_3:+63361658 None:intergenic
GCAGCTCGACCAAGCCCAAC+AGG 0.447516 4_3:+63363380 None:intergenic
TGTTTAACTTGAAACCTGTT+GGG 0.453678 4_3:-63363394 Msa0654970:CDS
GAATTAGGTTTAACGATGAC+GGG 0.453984 4_3:-63364392 Msa0654970:exon
AGAGTCGCGACGGAATCTTT+AGG 0.458235 4_3:-63364344 Msa0654970:CDS
CTGTTTAACTTGAAACCTGT+TGG 0.458401 4_3:-63363395 Msa0654970:CDS
GTCATCGTTAAACCTAATTC+CGG 0.465444 4_3:+63364395 None:intergenic
CGAAATTAAACTACGCAGAC+AGG 0.473262 4_3:-63361717 Msa0654970:CDS
GAGTTGAAAGCTATTAAAGA+TGG 0.477276 4_3:-63363638 Msa0654970:CDS
TTCTCCATAATTTATAAAGG+TGG 0.482341 4_3:-63361572 Msa0654970:three_prime_UTR
AACTTAAGCAAGCCTATCTT+CGG 0.486326 4_3:-63361670 Msa0654970:CDS
TGGCCAAACCAAGAAGAGAT+TGG 0.494066 4_3:-63361299 Msa0654970:three_prime_UTR
TTCTTGCAACACGTTGGGAT+TGG 0.494985 4_3:+63363469 None:intergenic
GGCCAGTCGTGCGAGAGAAA+AGG 0.496008 4_3:-63361744 Msa0654970:CDS
CGCGTTGATATTCGGCGTCA+GGG 0.499448 4_3:+63364186 None:intergenic
CTATGCTGCATTGGAGAAGA+AGG 0.501653 4_3:-63363606 Msa0654970:CDS
GCTGGTGATGGTGATGGTGA+TGG 0.502641 4_3:-63363431 Msa0654970:CDS
GAAAATGAACATGGTGGTGC+TGG 0.510141 4_3:-63363449 Msa0654970:CDS
AAGTCACTGAAATCAGTTGA+AGG 0.512826 4_3:+63361265 None:intergenic
GGGAGAATTGTCTGATGAAG+AGG 0.516669 4_3:-63363558 Msa0654970:CDS
GTCAGGGCCTGATAATTCTC+TGG 0.517650 4_3:+63364202 None:intergenic
CTTCCATCCGTGTAAAATTG+AGG 0.520705 4_3:-63364472 None:intergenic
ATCAGTGAGCTATGCTGCAT+TGG 0.523981 4_3:-63363615 Msa0654970:CDS
GACGGGGACGGAGGCACCGT+CGG 0.533538 4_3:-63364375 Msa0654970:CDS
GAAGAAATTGGCAGAACTGA+AGG 0.534198 4_3:-63361648 Msa0654970:CDS
TGAAATCAGTTGAAGGTCAA+AGG 0.545364 4_3:+63361272 None:intergenic
GCAGAACTGAAGGCCACTGT+TGG 0.546526 4_3:-63361638 Msa0654970:CDS
AGGTTTAACGATGACGGGGA+CGG 0.548885 4_3:-63364387 Msa0654970:exon
GGTGGTGCTGGTGATGGTGA+TGG 0.554003 4_3:-63363437 Msa0654970:CDS
GCGACTCTCTTCTTCTCCGA+CGG 0.562550 4_3:+63364359 None:intergenic
ACCGAGTTATTATTATTCGT+CGG 0.563720 4_3:-63361491 Msa0654970:three_prime_UTR
CGAGCTGCTGGAGTAGTTGA+CGG 0.566239 4_3:-63363365 Msa0654970:CDS
GAAGAAGAGAGAAAATTCAC+CGG 0.574648 4_3:-63364414 Msa0654970:five_prime_UTR
GGAATTAGGTTTAACGATGA+CGG 0.574948 4_3:-63364393 Msa0654970:exon
TTATAAAGGTGGATAGAGAG+AGG 0.575668 4_3:-63361561 Msa0654970:three_prime_UTR
GAACATGGTGGTGCTGGTGA+TGG 0.586272 4_3:-63363443 Msa0654970:CDS
CACAGAACCATCGAGGGTGT+TGG 0.591588 4_3:-63364287 Msa0654970:CDS
GAAATAGGAACCGAGCGCAA+CGG 0.599113 4_3:-63364450 Msa0654970:five_prime_UTR
TTGCAGGGAAGTTCATGAAG+AGG 0.605605 4_3:-63361765 Msa0654970:intron
AATACACATGATGATAATAG+GGG 0.610759 4_3:+63361206 None:intergenic
CACATGATGATAATAGGGGA+TGG 0.619085 4_3:+63361210 None:intergenic
AGATGCACCAACACCCTCGA+TGG 0.631777 4_3:+63364280 None:intergenic
ATTCGTCGGAGTTACAACAT+TGG 0.639519 4_3:-63361477 Msa0654970:three_prime_UTR
TCGCGACGGAATCTTTAGGA+TGG 0.640809 4_3:-63364340 Msa0654970:CDS
CCACTGTTATCATGTTCGTG+TGG 0.646791 4_3:-63361238 Msa0654970:three_prime_UTR
GAGATCGCGTGCATCGACAC+CGG 0.655586 4_3:+63364118 None:intergenic
TTTAACGATGACGGGGACGG+AGG 0.656467 4_3:-63364384 Msa0654970:exon
GGAATCTTTAGGATGGCTAA+CGG 0.658502 4_3:-63364333 Msa0654970:CDS
AAGAAGCACAGAACCATCGA+GGG 0.672564 4_3:-63364293 Msa0654970:CDS
GGAGAAGAAGAGAGTCGCGA+CGG 0.681156 4_3:-63364354 Msa0654970:CDS
TCCGACGAATAATAATAACT+CGG 0.691834 4_3:+63361490 None:intergenic
CCACACGAACATGATAACAG+TGG 0.692369 4_3:+63361238 None:intergenic
AAAGAAGCACAGAACCATCG+AGG 0.694787 4_3:-63364294 Msa0654970:CDS
AATTAGGTTTAACGATGACG+GGG 0.716816 4_3:-63364391 Msa0654970:exon
CTCAGATTCAGATCCAACAG+TGG 0.724935 4_3:+63361625 None:intergenic
GTGTTGCAAGAAAATGAACA+TGG 0.731258 4_3:-63363458 Msa0654970:CDS
TTGCAAGAAAATGAACATGG+TGG 0.793243 4_3:-63363455 Msa0654970:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTATTTTAATTGTTC+AGG - chr4_3:63361551-63361570 Msa0654970:three_prime_UTR 10.0%
!! AAAAAAAAAAAACTCTTTCT+AGG - chr4_3:63363574-63363593 Msa0654970:CDS 15.0%
!!! TTTTAAAAATTTTGATTTGC+AGG - chr4_3:63363820-63363839 Msa0654970:intron 15.0%
!!! TTTAAAAATTTTGATTTGCA+GGG - chr4_3:63363821-63363840 Msa0654970:intron 15.0%
!! TTATTCTCCATAATTTATAA+AGG - chr4_3:63364026-63364045 Msa0654970:intron 15.0%
!! AAACATAAAGTGAAAGAAAA+GGG + chr4_3:63361525-63361544 None:intergenic 20.0%
!! TTTGAATATAAATTTGAGTC+TGG - chr4_3:63361706-63361725 Msa0654970:CDS 20.0%
!! TAAAGATATAAGTGTATGTT+TGG + chr4_3:63361848-63361867 None:intergenic 20.0%
!!! AATTTGTGTTTGTTGATAAT+TGG - chr4_3:63361883-63361902 Msa0654970:intron 20.0%
!!! ATTTGTGTTTGTTGATAATT+GGG - chr4_3:63361884-63361903 Msa0654970:intron 20.0%
!! TTGTATGATAAGTTAGTTAA+GGG - chr4_3:63362023-63362042 Msa0654970:intron 20.0%
!!! CAATGTTTTTGTTCTATATA+TGG - chr4_3:63362312-63362331 Msa0654970:intron 20.0%
!!! TTTTTGTTCTATATATGGTT+AGG - chr4_3:63362317-63362336 Msa0654970:intron 20.0%
!!! TTCTTTGAAAACTTAACTTT+TGG - chr4_3:63362369-63362388 Msa0654970:intron 20.0%
!! ACCAAAATATGCTTTATATA+TGG - chr4_3:63362530-63362549 Msa0654970:intron 20.0%
!! CCAAAATATGCTTTATATAT+GGG - chr4_3:63362531-63362550 Msa0654970:intron 20.0%
!! AATAATCTATGTGAATACAA+TGG - chr4_3:63362976-63362995 Msa0654970:intron 20.0%
!! TAATGACTTACTATAAGTAA+CGG - chr4_3:63363527-63363546 Msa0654970:CDS 20.0%
!!! TTTGTCTTTTACATTTAAGT+AGG - chr4_3:63363632-63363651 Msa0654970:CDS 20.0%
!!! TTGTCTTTTACATTTAAGTA+GGG - chr4_3:63363633-63363652 Msa0654970:CDS 20.0%
!! AAGTAACAAGTAACAATAAT+AGG + chr4_3:63363690-63363709 None:intergenic 20.0%
!!! TTTTTTATCCATGATTATTC+TGG - chr4_3:63363752-63363771 Msa0654970:intron 20.0%
!!! TTTTTATCCATGATTATTCT+GGG - chr4_3:63363753-63363772 Msa0654970:intron 20.0%
!!! GCAAATATTTCCTTTAAAAA+AGG - chr4_3:63364062-63364081 Msa0654970:intron 20.0%
!!! TTTCTTTTTTGATTATCTGA+GGG + chr4_3:63364179-63364198 None:intergenic 20.0%
!! TTATTTACAAGAGAAAAAAG+AGG + chr4_3:63364228-63364247 None:intergenic 20.0%
!! CTATGTACATGAATTATTAT+AGG - chr4_3:63364264-63364283 Msa0654970:CDS 20.0%
!!! ATAGGTAAATTTAGTTTTGT+TGG - chr4_3:63364282-63364301 Msa0654970:CDS 20.0%
!! AAATACACATGATGATAATA+GGG + chr4_3:63364399-63364418 None:intergenic 20.0%
! GAAACATAAAGTGAAAGAAA+AGG + chr4_3:63361526-63361545 None:intergenic 25.0%
! TACAGTGAGAAAAATTGATT+TGG - chr4_3:63361620-63361639 Msa0654970:CDS 25.0%
!! TGCTTTTGTAAATTTGATTC+TGG - chr4_3:63361650-63361669 Msa0654970:CDS 25.0%
! AGAATCAAAGCTCAAAATTT+AGG - chr4_3:63361740-63361759 Msa0654970:CDS 25.0%
! CAACAAACACAAATTCAATT+AGG + chr4_3:63361878-63361897 None:intergenic 25.0%
!!! TGTGTTTGTTGTTATGATTA+GGG - chr4_3:63361932-63361951 Msa0654970:intron 25.0%
! GTTGTATGATAAGTTAGTTA+AGG - chr4_3:63362022-63362041 Msa0654970:intron 25.0%
!! CTTTTGGATGATATTCATTT+TGG - chr4_3:63362385-63362404 Msa0654970:intron 25.0%
! CTTTGTATGAAATGTGATTT+CGG - chr4_3:63362457-63362476 Msa0654970:intron 25.0%
!! CCCATATATAAAGCATATTT+TGG + chr4_3:63362534-63362553 None:intergenic 25.0%
! TACGTATTACAAGTAAAACT+TGG - chr4_3:63362689-63362708 Msa0654970:intron 25.0%
!!! TCTTCTAGAGCTTTTAATTT+TGG - chr4_3:63363386-63363405 Msa0654970:CDS 25.0%
!!! CTTTTAATTTTGGCTTTAGA+GGG - chr4_3:63363396-63363415 Msa0654970:CDS 25.0%
!!! TTTTAATTTTGGCTTTAGAG+GGG - chr4_3:63363397-63363416 Msa0654970:CDS 25.0%
!! AAAATTTGACTTAATCCAGT+AGG + chr4_3:63363437-63363456 None:intergenic 25.0%
!! ACTGGATTAAGTCAAATTTT+TGG - chr4_3:63363437-63363456 Msa0654970:CDS 25.0%
! CTTAAATGTAAAAGACAAAC+AGG + chr4_3:63363631-63363650 None:intergenic 25.0%
!!! TGTTACTTGTTACTTTTTTC+CGG - chr4_3:63363695-63363714 Msa0654970:intron 25.0%
! TTCTCCATAATTTATAAAGG+TGG - chr4_3:63364029-63364048 Msa0654970:intron 25.0%
! CTATCCACCTTTATAAATTA+TGG + chr4_3:63364036-63364055 None:intergenic 25.0%
!!! CTTTCTTTTTTGATTATCTG+AGG + chr4_3:63364180-63364199 None:intergenic 25.0%
! AATACACATGATGATAATAG+GGG + chr4_3:63364398-63364417 None:intergenic 25.0%
! CAAATACACATGATGATAAT+AGG + chr4_3:63364400-63364419 None:intergenic 25.0%
TTCAGCTTTCAATTCGAAAA+TGG + chr4_3:63361348-63361367 None:intergenic 30.0%
! TGATAATTCTCTGGACTTTT+TGG + chr4_3:63361393-63361412 None:intergenic 30.0%
AAAAAGTCCAGAGAATTATC+AGG - chr4_3:63361392-63361411 Msa0654970:three_prime_UTR 30.0%
!! TTTGGAAGTGATGATGTTTT+TGG + chr4_3:63361441-63361460 None:intergenic 30.0%
AAAAACATCATCACTTCCAA+AGG - chr4_3:63361440-63361459 Msa0654970:three_prime_UTR 30.0%
TTTACTTTCAACTCAGGTTT+CGG + chr4_3:63361676-63361695 None:intergenic 30.0%
AATCACTTTACTTTCAACTC+AGG + chr4_3:63361682-63361701 None:intergenic 30.0%
!!! ACTTTTGGAGCAATTTTAAC+AGG + chr4_3:63361821-63361840 None:intergenic 30.0%
TGTTAAAATTGCTCCAAAAG+TGG - chr4_3:63361820-63361839 Msa0654970:intron 30.0%
!! GTGTGTTTGTTGTTATGATT+AGG - chr4_3:63361931-63361950 Msa0654970:intron 30.0%
GAGTTGAAAGCTATTAAAGA+TGG - chr4_3:63361963-63361982 Msa0654970:intron 30.0%
!! TGTTTAACTTGAAACCTGTT+GGG - chr4_3:63362207-63362226 Msa0654970:intron 30.0%
GTTCTATATATGGTTAGGAT+AGG - chr4_3:63362322-63362341 Msa0654970:intron 30.0%
TAAGAAAATGTTTGAGAGAG+TGG - chr4_3:63362737-63362756 Msa0654970:intron 30.0%
! ATGATGATGATCAAGATTAC+AGG - chr4_3:63362792-63362811 Msa0654970:intron 30.0%
! TGATGATGATCAAGATTACA+GGG - chr4_3:63362793-63362812 Msa0654970:intron 30.0%
TGTTTATCATATCCCATTTG+TGG + chr4_3:63363178-63363197 None:intergenic 30.0%
TTAACTACTTGTGCTTAGTT+TGG - chr4_3:63363241-63363260 Msa0654970:intron 30.0%
TAACTACTTGTGCTTAGTTT+GGG - chr4_3:63363242-63363261 Msa0654970:intron 30.0%
AGTATCCTTCTTGTTAGAAT+TGG - chr4_3:63363313-63363332 Msa0654970:intron 30.0%
!!! GCTTTTAATTTTGGCTTTAG+AGG - chr4_3:63363395-63363414 Msa0654970:CDS 30.0%
!!! GTTACTTGTTACTTTTTTCC+GGG - chr4_3:63363696-63363715 Msa0654970:intron 30.0%
!!! TTACTTGTTACTTTTTTCCG+GGG - chr4_3:63363697-63363716 Msa0654970:intron 30.0%
! TTTCCTTTAAAAAAGGCATG+AGG - chr4_3:63364069-63364088 Msa0654970:intron 30.0%
ACCGAGTTATTATTATTCGT+CGG - chr4_3:63364110-63364129 Msa0654970:intron 30.0%
TCCGACGAATAATAATAACT+CGG + chr4_3:63364114-63364133 None:intergenic 30.0%
GAAGAAGAGAGAAAATTCAC+CGG - chr4_3:63361187-63361206 Msa0654970:three_prime_UTR 35.0%
AGAGAAAATTCACCGGAATT+AGG - chr4_3:63361194-63361213 Msa0654970:three_prime_UTR 35.0%
GTCATCGTTAAACCTAATTC+CGG + chr4_3:63361209-63361228 None:intergenic 35.0%
GGAATTAGGTTTAACGATGA+CGG - chr4_3:63361208-63361227 Msa0654970:three_prime_UTR 35.0%
GAATTAGGTTTAACGATGAC+GGG - chr4_3:63361209-63361228 Msa0654970:three_prime_UTR 35.0%
AATTAGGTTTAACGATGACG+GGG - chr4_3:63361210-63361229 Msa0654970:three_prime_UTR 35.0%
TTTATTGGAATAGGGACCTT+TGG + chr4_3:63361459-63361478 None:intergenic 35.0%
! CCGGAGTTTTTATTGGAATA+GGG + chr4_3:63361467-63361486 None:intergenic 35.0%
CCCTATTCCAATAAAAACTC+CGG - chr4_3:63361464-63361483 Msa0654970:three_prime_UTR 35.0%
! ACCGGAGTTTTTATTGGAAT+AGG + chr4_3:63361468-63361487 None:intergenic 35.0%
AAGTGAAAGAAAAGGGAAAC+AGG + chr4_3:63361518-63361537 None:intergenic 35.0%
! AAGCTCAAAATTTAGGTTCG+AGG - chr4_3:63361747-63361766 Msa0654970:CDS 35.0%
GTTCGAGGTAGAATAGATTA+TGG - chr4_3:63361762-63361781 Msa0654970:CDS 35.0%
!! TGGAGCAATTTTAACAGGTT+TGG + chr4_3:63361816-63361835 None:intergenic 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG + chr4_3:63361836-63361855 None:intergenic 35.0%
! TCATTTTCTTGCAACACGTT+GGG + chr4_3:63362140-63362159 None:intergenic 35.0%
! TTCATTTTCTTGCAACACGT+TGG + chr4_3:63362141-63362160 None:intergenic 35.0%
GTGTTGCAAGAAAATGAACA+TGG - chr4_3:63362143-63362162 Msa0654970:intron 35.0%
TTGCAAGAAAATGAACATGG+TGG - chr4_3:63362146-63362165 Msa0654970:intron 35.0%
!! CTGTTTAACTTGAAACCTGT+TGG - chr4_3:63362206-63362225 Msa0654970:intron 35.0%
TGTATGAAATGTGATTTCGG+AGG - chr4_3:63362460-63362479 Msa0654970:intron 35.0%
AGTGATAGCAATGATTTGAG+AGG - chr4_3:63362554-63362573 Msa0654970:intron 35.0%
TTCTAACCTTGAAGCTTGAA+AGG + chr4_3:63362583-63362602 None:intergenic 35.0%
! GAAGTGTCAAAATTTCTGTG+AGG - chr4_3:63362600-63362619 Msa0654970:intron 35.0%
ATATAGAAGTCCAGTGAGAT+TGG - chr4_3:63362834-63362853 Msa0654970:intron 35.0%
! TTTGCTACTTCTGTTAACCT+AGG + chr4_3:63362918-63362937 None:intergenic 35.0%
AATACCCACATCGTAATTCA+AGG - chr4_3:63363050-63363069 Msa0654970:intron 35.0%
! TTAGTTTGGGAGTGTAGAAT+AGG - chr4_3:63363255-63363274 Msa0654970:intron 35.0%
! TAGTTTGGGAGTGTAGAATA+GGG - chr4_3:63363256-63363275 Msa0654970:intron 35.0%
ATGTGCCAATTCTAACAAGA+AGG + chr4_3:63363321-63363340 None:intergenic 35.0%
TCTTGTTAGAATTGGCACAT+AGG - chr4_3:63363321-63363340 Msa0654970:intron 35.0%
! CTCTAGAAGAGTGAATAAAC+GGG + chr4_3:63363376-63363395 None:intergenic 35.0%
!! GCTCTAGAAGAGTGAATAAA+CGG + chr4_3:63363377-63363396 None:intergenic 35.0%
TATAGTGCTGTAACCTATTG+AGG - chr4_3:63363500-63363519 Msa0654970:CDS 35.0%
AAGTCATTACCTACCTCAAT+AGG + chr4_3:63363516-63363535 None:intergenic 35.0%
TATAAGTAACGGCTCTTTCT+AGG - chr4_3:63363538-63363557 Msa0654970:CDS 35.0%
TAAGTAGGGAATGATGAAGA+TGG - chr4_3:63363647-63363666 Msa0654970:CDS 35.0%
AAGTAGGGAATGATGAAGAT+GGG - chr4_3:63363648-63363667 Msa0654970:CDS 35.0%
!!! TACTTGTTACTTTTTTCCGG+GGG - chr4_3:63363698-63363717 Msa0654970:intron 35.0%
GCAATTACCCAGAATAATCA+TGG + chr4_3:63363763-63363782 None:intergenic 35.0%
TCGTTAAATTTGTGAGTCTG+TGG - chr4_3:63363784-63363803 Msa0654970:intron 35.0%
! CTGTCTGCGTAGTTTAATTT+CGG + chr4_3:63363886-63363905 None:intergenic 35.0%
AACTTAAGCAAGCCTATCTT+CGG - chr4_3:63363931-63363950 Msa0654970:intron 35.0%
TTATAAAGGTGGATAGAGAG+AGG - chr4_3:63364040-63364059 Msa0654970:intron 35.0%
!!! ACTCCTCATGCCTTTTTTAA+AGG + chr4_3:63364075-63364094 None:intergenic 35.0%
AGTTACAACATTGGTGTATC+TGG - chr4_3:63364133-63364152 Msa0654970:CDS 35.0%
TGAAATCAGTTGAAGGTCAA+AGG + chr4_3:63364332-63364351 None:intergenic 35.0%
AAGTCACTGAAATCAGTTGA+AGG + chr4_3:63364339-63364358 None:intergenic 35.0%
!! GGAATCTTTAGGATGGCTAA+CGG - chr4_3:63361268-63361287 Msa0654970:three_prime_UTR 40.0%
!!! GTTTTTGGCGCGTTGATATT+CGG + chr4_3:63361426-63361445 None:intergenic 40.0%
! ATCGACACCGGAGTTTTTAT+TGG + chr4_3:63361474-63361493 None:intergenic 40.0%
CTGTAGAATCAAACACACAG+CGG + chr4_3:63361605-63361624 None:intergenic 40.0%
CGAGGTAGAATAGATTATGG+AGG - chr4_3:63361765-63361784 Msa0654970:intron 40.0%
ATACAACACACACTCCTTCA+AGG + chr4_3:63362427-63362446 None:intergenic 40.0%
! AGGAGTGTGTGTTGTATGTT+TGG - chr4_3:63362430-63362449 Msa0654970:intron 40.0%
TCGGAGGAATTAATGTCGTT+TGG - chr4_3:63362476-63362495 Msa0654970:intron 40.0%
AGGTTTCCTTTCAAGCTTCA+AGG - chr4_3:63362574-63362593 Msa0654970:intron 40.0%
ATGCGGAAGCTAAATCTGTT+TGG - chr4_3:63362636-63362655 Msa0654970:intron 40.0%
! TAAAACTTGGTGACAGAGTG+AGG - chr4_3:63362702-63362721 Msa0654970:intron 40.0%
! AAAACTTGGTGACAGAGTGA+GGG - chr4_3:63362703-63362722 Msa0654970:intron 40.0%
ATACCAGTTGCAAGAATCCT+AGG - chr4_3:63362898-63362917 Msa0654970:intron 40.0%
TAACCTAGGATTCTTGCAAC+TGG + chr4_3:63362904-63362923 None:intergenic 40.0%
TCCACCTTGAATTACGATGT+GGG + chr4_3:63363057-63363076 None:intergenic 40.0%
!!! AGTGTTGGACTTTTTTGCCA+TGG + chr4_3:63363084-63363103 None:intergenic 40.0%
GCAAAAAAGTCCAACACTTC+CGG - chr4_3:63363086-63363105 Msa0654970:intron 40.0%
CAAAAAAGTCCAACACTTCC+GGG - chr4_3:63363087-63363106 Msa0654970:intron 40.0%
GCTACACATAGAGAAAATCC+CGG + chr4_3:63363108-63363127 None:intergenic 40.0%
TATCCCATTTGTGGATCAAC+TGG + chr4_3:63363169-63363188 None:intergenic 40.0%
TGTAGAATAGGGCTGAGTTA+AGG - chr4_3:63363267-63363286 Msa0654970:intron 40.0%
AGTAGGGAATGATGAAGATG+GGG - chr4_3:63363649-63363668 Msa0654970:CDS 40.0%
TTGTGAGTCTGTGGCTAATT+TGG - chr4_3:63363793-63363812 Msa0654970:intron 40.0%
CGAAATTAAACTACGCAGAC+AGG - chr4_3:63363884-63363903 Msa0654970:intron 40.0%
AGCCTATCTTCGGAAGAAAT+TGG - chr4_3:63363941-63363960 Msa0654970:intron 40.0%
TGCCAATTTCTTCCGAAGAT+AGG + chr4_3:63363946-63363965 None:intergenic 40.0%
GAAGAAATTGGCAGAACTGA+AGG - chr4_3:63363953-63363972 Msa0654970:intron 40.0%
ATTCGTCGGAGTTACAACAT+TGG - chr4_3:63364124-63364143 Msa0654970:CDS 40.0%
AGACCAATCTCTTCTTGGTT+TGG + chr4_3:63364308-63364327 None:intergenic 40.0%
AAGGAAGACCAATCTCTTCT+TGG + chr4_3:63364313-63364332 None:intergenic 40.0%
CACATGATGATAATAGGGGA+TGG + chr4_3:63364394-63364413 None:intergenic 40.0%
! TTTTGTTCTTCCGTTGCGCT+CGG + chr4_3:63361164-63361183 None:intergenic 45.0%
! TCGATGGTTCTGTGCTTCTT+TGG + chr4_3:63361308-63361327 None:intergenic 45.0%
! AAAGAAGCACAGAACCATCG+AGG - chr4_3:63361307-63361326 Msa0654970:three_prime_UTR 45.0%
! AAGAAGCACAGAACCATCGA+GGG - chr4_3:63361308-63361327 Msa0654970:three_prime_UTR 45.0%
ATCAGTGAGCTATGCTGCAT+TGG - chr4_3:63361986-63362005 Msa0654970:intron 45.0%
CTATGCTGCATTGGAGAAGA+AGG - chr4_3:63361995-63362014 Msa0654970:intron 45.0%
GGGAGAATTGTCTGATGAAG+AGG - chr4_3:63362043-63362062 Msa0654970:intron 45.0%
TTCTTGCAACACGTTGGGAT+TGG + chr4_3:63362135-63362154 None:intergenic 45.0%
! GAAAATGAACATGGTGGTGC+TGG - chr4_3:63362152-63362171 Msa0654970:intron 45.0%
AACTTGAAACCTGTTGGGCT+TGG - chr4_3:63362212-63362231 Msa0654970:intron 45.0%
! AAAGTGCTTGTGAGCCTTGA+AGG - chr4_3:63362410-63362429 Msa0654970:intron 45.0%
! AAACTTGGTGACAGAGTGAG+GGG - chr4_3:63362704-63362723 Msa0654970:intron 45.0%
ACCCACATCGTAATTCAAGG+TGG - chr4_3:63363053-63363072 Msa0654970:intron 45.0%
GTCCACCTTGAATTACGATG+TGG + chr4_3:63363058-63363077 None:intergenic 45.0%
AATTCAAGGTGGACACACCA+TGG - chr4_3:63363064-63363083 Msa0654970:intron 45.0%
! AGAGAAAATCCCGGAAGTGT+TGG + chr4_3:63363099-63363118 None:intergenic 45.0%
ATGTGTAGCAAGTCTCTGAG+TGG - chr4_3:63363119-63363138 Msa0654970:intron 45.0%
AGCAAGTCTCTGAGTGGATA+AGG - chr4_3:63363125-63363144 Msa0654970:intron 45.0%
TCAGCCAGTTGATCCACAAA+TGG - chr4_3:63363162-63363181 Msa0654970:intron 45.0%
CAGCCAGTTGATCCACAAAT+GGG - chr4_3:63363163-63363182 Msa0654970:intron 45.0%
GTCAGTTGTTGAATGCCTAC+TGG - chr4_3:63363419-63363438 Msa0654970:CDS 45.0%
GTGCTGTAACCTATTGAGGT+AGG - chr4_3:63363504-63363523 Msa0654970:CDS 45.0%
!!! TTGTTACTTTTTTCCGGGGG+TGG - chr4_3:63363701-63363720 Msa0654970:intron 45.0%
!!! TGTTACTTTTTTCCGGGGGT+GGG - chr4_3:63363702-63363721 Msa0654970:intron 45.0%
TTGCAGGGAAGTTCATGAAG+AGG - chr4_3:63363836-63363855 Msa0654970:intron 45.0%
CTCAGATTCAGATCCAACAG+TGG + chr4_3:63363979-63363998 None:intergenic 45.0%
TGGCCAAACCAAGAAGAGAT+TGG - chr4_3:63364302-63364321 Msa0654970:CDS 45.0%
CCACACGAACATGATAACAG+TGG + chr4_3:63364366-63364385 None:intergenic 45.0%
CCACTGTTATCATGTTCGTG+TGG - chr4_3:63364363-63364382 Msa0654970:CDS 45.0%
GAAATAGGAACCGAGCGCAA+CGG - chr4_3:63361151-63361170 Msa0654970:three_prime_UTR 50.0%
!! AGGTTTAACGATGACGGGGA+CGG - chr4_3:63361214-63361233 Msa0654970:three_prime_UTR 50.0%
AGAGTCGCGACGGAATCTTT+AGG - chr4_3:63361257-63361276 Msa0654970:three_prime_UTR 50.0%
! TCGCGACGGAATCTTTAGGA+TGG - chr4_3:63361261-63361280 Msa0654970:three_prime_UTR 50.0%
GTCAGGGCCTGATAATTCTC+TGG + chr4_3:63361402-63361421 None:intergenic 50.0%
CTGAGCACAAGCGCAATGTT+AGG - chr4_3:63362261-63362280 Msa0654970:intron 50.0%
GAGGAGCTTGTGCTTCAATG+CGG - chr4_3:63362619-63362638 Msa0654970:intron 50.0%
GGAATGATGAAGATGGGGCT+GGG - chr4_3:63363654-63363673 Msa0654970:intron 50.0%
!! TTTAACGATGACGGGGACGG+AGG - chr4_3:63361217-63361236 Msa0654970:three_prime_UTR 55.0%
GCGACTCTCTTCTTCTCCGA+CGG + chr4_3:63361245-63361264 None:intergenic 55.0%
GGAGAAGAAGAGAGTCGCGA+CGG - chr4_3:63361247-63361266 Msa0654970:three_prime_UTR 55.0%
! CACAGAACCATCGAGGGTGT+TGG - chr4_3:63361314-63361333 Msa0654970:three_prime_UTR 55.0%
AGATGCACCAACACCCTCGA+TGG + chr4_3:63361324-63361343 None:intergenic 55.0%
! CGCGTTGATATTCGGCGTCA+GGG + chr4_3:63361418-63361437 None:intergenic 55.0%
!! GAACATGGTGGTGCTGGTGA+TGG - chr4_3:63362158-63362177 Msa0654970:intron 55.0%
!! GCTGGTGATGGTGATGGTGA+TGG - chr4_3:63362170-63362189 Msa0654970:intron 55.0%
CGAGCTGCTGGAGTAGTTGA+CGG - chr4_3:63362236-63362255 Msa0654970:intron 55.0%
GAAGCAGCGACCAATCTCAC+TGG + chr4_3:63362847-63362866 None:intergenic 55.0%
GGGAATGATGAAGATGGGGC+TGG - chr4_3:63363653-63363672 Msa0654970:intron 55.0%
! GGGTGGGAGCTTGAGGTTTT+TGG - chr4_3:63363718-63363737 Msa0654970:intron 55.0%
! AGCCTTTTCTCTCGCACGAC+TGG + chr4_3:63363862-63363881 None:intergenic 55.0%
GCAGAACTGAAGGCCACTGT+TGG - chr4_3:63363963-63363982 Msa0654970:intron 55.0%
! GCGCGTTGATATTCGGCGTC+AGG + chr4_3:63361419-63361438 None:intergenic 60.0%
! GAGATCGCGTGCATCGACAC+CGG + chr4_3:63361486-63361505 None:intergenic 60.0%
!! GGTGGTGCTGGTGATGGTGA+TGG - chr4_3:63362164-63362183 Msa0654970:intron 60.0%
AAACCTCAAGCTCCCACCCC+CGG + chr4_3:63363717-63363736 None:intergenic 60.0%
GGCCAGTCGTGCGAGAGAAA+AGG - chr4_3:63363857-63363876 Msa0654970:intron 60.0%
GCAGCTCGACCAAGCCCAAC+AGG + chr4_3:63362224-63362243 None:intergenic 65.0%
! GTTGGGCTTGGTCGAGCTGC+TGG - chr4_3:63362224-63362243 Msa0654970:intron 65.0%
TTTCCGGGGGTGGGAGCTTG+AGG - chr4_3:63363711-63363730 Msa0654970:intron 65.0%
!! GACGGGGACGGAGGCACCGT+CGG - chr4_3:63361226-63361245 Msa0654970:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 63361140 63364483 63361140 ID=Msa0654970;Name=Msa0654970
chr4_3 mRNA 63361140 63364483 63361140 ID=Msa0654970-mRNA-1;Parent=Msa0654970;Name=Msa0654970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=84|1|1|1|1|1|3|470|261
chr4_3 exon 63361140 63361781 63361140 ID=Msa0654970-mRNA-1:exon:17776;Parent=Msa0654970-mRNA-1
chr4_3 exon 63363341 63363670 63363341 ID=Msa0654970-mRNA-1:exon:17775;Parent=Msa0654970-mRNA-1
chr4_3 exon 63364116 63364483 63364116 ID=Msa0654970-mRNA-1:exon:17774;Parent=Msa0654970-mRNA-1
chr4_3 five_prime_UTR 63364400 63364483 63364400 ID=Msa0654970-mRNA-1:five_prime_utr;Parent=Msa0654970-mRNA-1
chr4_3 CDS 63364116 63364399 63364116 ID=Msa0654970-mRNA-1:cds;Parent=Msa0654970-mRNA-1
chr4_3 CDS 63363341 63363670 63363341 ID=Msa0654970-mRNA-1:cds;Parent=Msa0654970-mRNA-1
chr4_3 CDS 63361610 63361781 63361610 ID=Msa0654970-mRNA-1:cds;Parent=Msa0654970-mRNA-1
chr4_3 three_prime_UTR 63361140 63361609 63361140 ID=Msa0654970-mRNA-1:three_prime_utr;Parent=Msa0654970-mRNA-1
Gene Sequence

>Msa0654970

ATGACGGGGACGGAGGCACCGTCGGAGAAGAAGAGAGTCGCGACGGAATCTTTAGGATGGCTAACGGAATCGACGATAATGCCAAAGAAGCACAGAACCATCGAGGGTGTTGGTGCATCTTCCATTTTCGAATTGAAAGCTGAACTCTACAAATCTCAAGAAGATTCCAAAAAGTCCAGAGAATTATCAGGCCCTGACGCCGAATATCAACGCGCCAAAAACATCATCACTTCCAAAGGTCCCTATTCCAATAAAAACTCCGGTGTCGATGCACGCGATCTCAAGGATAAACTTGAGTTGAAAGCTATTAAAGATGGATCAGTGAGCTATGCTGCATTGGAGAAGAAGGCTGAGTTGTATGATAAGTTAGTTAAGGGAGAATTGTCTGATGAAGAGGATAAAGAGAAGTATTGTGTTGATTTTGTTCGTAAGAGCGATGATCATGATGATGATAATGTCTCTACCAATCCCAACGTGTTGCAAGAAAATGAACATGGTGGTGCTGGTGATGGTGATGGTGATGGTGATTCTTTTTCGCTGTTTAACTTGAAACCTGTTGGGCTTGGTCGAGCTGCTGGAGTAGTTGACGGTGCTGAGCACAAGCGCAATGTTAGGGAAGTTCATGAAGAGGCCAGTCGTGCGAGAGAAAAGGCTTCCGAAATTAAACTACGCAGACAGGAGCAGTTAGCTGCTCAACGCGAGAAACTTAAGCAAGCCTATCTTCGGAAGAAATTGGCAGAACTGAAGGCCACTGTTGGATCTGAATCTGAGATTAAGCACACATGA
Protein sequence

>Msa0654970

MTGTEAPSEKKRVATESLGWLTESTIMPKKHRTIEGVGASSIFELKAELYKSQEDSKKSRELSGPDAEYQRAKNIITSKGPYSNKNSGVDARDLKDKLELKAIKDGSVSYAALEKKAELYDKLVKGELSDEEDKEKYCVDFVRKSDDHDDDNVSTNPNVLQENEHGGAGDGDGDGDSFSLFNLKPVGLGRAAGVVDGAEHKRNVREVHEEASRAREKASEIKLRRQEQLAAQREKLKQAYLRKKLAELKATVGSESEIKHT*