AlfalfaGEDB | Msa0629400

M. sativa cultivar zhongmu-4 / Msa0629400

KEGG: K03231
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: PF00009.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0629400	XP_003604587.1	99.776	447	1	0	1	447	1	447	0.0	919

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0629400	sp\|O49169\|EF1A_MANES	94.395	446	25	0	1	446	1	446	0.0	879

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0629400	G7JLV1	99.776	447	1	0	1	447	1	447	0.0	919

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032040	Msa0629400	-0.809058	2.283125e-50	-8.615850e-47
Msa0331830	Msa0629400	-0.805262	1.453510e-49	-8.615850e-47
Msa0587060	Msa0629400	-0.809292	2.033903e-50	-8.615850e-47
Msa0589440	Msa0629400	0.960583	1.011778e-118	-8.615850e-47
Msa0626890	Msa0629400	-0.809427	1.902920e-50	-8.615850e-47
Msa0629400	Msa0659130	-0.800703	1.272613e-48	-8.615850e-47
Msa0629400	Msa0665010	-0.811427	7.041122e-51	-8.615850e-47
Msa0629400	Msa0705610	-0.800593	1.339882e-48	-8.615850e-47
Msa0629400	Msa1385940	-0.806195	9.256263e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0629400	MtrunA17_Chr4g0005781	99.776	447	1	0	1	447	1	447	919
Msa0629400	MtrunA17_Chr1g0200071	96.429	448	15	1	1	447	1	448	891
Msa0629400	MtrunA17_Chr1g0200091	96.429	448	15	1	1	447	1	448	891
Msa0629400	MtrunA17_Chr1g0200121	96.205	448	16	1	1	447	1	448	890
Msa0629400	MtrunA17_Chr6g0458111	96.083	434	17	0	1	434	1	434	874
Msa0629400	MtrunA17_Chr6g0458091	96.083	434	17	0	1	434	1	434	874
Msa0629400	MtrunA17_Chr1g0150331	88.248	451	23	3	1	447	1	425	800
Msa0629400	MtrunA17_Chr1g0193591	82.258	434	77	0	1	434	1	434	743
Msa0629400	MtrunA17_Chr1g0204031	80.963	436	78	3	1	436	1	431	731
Msa0629400	MtrunA17_Chr1g0156421	78.802	434	92	0	1	434	1	434	726

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	bit score
Msa0629400	AT1G07920.1	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07940.4	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07940.1	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07940.2	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07940.3	93.946	446	27	1	446	1	446	877
Msa0629400	AT5G60390.3	93.946	446	27	1	446	1	446	877
Msa0629400	AT5G60390.1	93.946	446	27	1	446	1	446	877
Msa0629400	AT5G60390.2	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07930.1	93.946	446	27	1	446	1	446	877
Msa0629400	AT1G07930.2	98.039	255	5	1	255	1	255	528

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Find 155 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGGGATACAAAGTGAA+GGG	0.623909	4_3:+10264974	None:intergenic
AAACCAGAGATGGGAACGAA+TGG	0.584939	4_3:+10263032	None:intergenic
AAACTTGACAGCAATGTGGG+AGG	0.695398	4_3:+10262539	None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG	0.559179	4_3:+10263298	None:intergenic
AAGGACAATGGGTAAGGAAA+AGG	0.424225	4_3:-10263703	Msa0629400:exon
AAGTTAGCAGCCTCCTTGGC+TGG	0.316454	4_3:+10262636	None:intergenic
AATGAAATCCCTGTGTCCGG+GGG	0.661737	4_3:+10263422	None:intergenic
AATGATCTGCTGCTGCAACA+AGG	0.643325	4_3:-10263256	Msa0629400:intron
AATTTCATCATACCTGCTCT+TGG	0.430266	4_3:+10263109	None:intergenic
ACAAATCTTGACTGGTACAA+GGG	0.563768	4_3:-10262981	Msa0629400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAGGGTTTATATATAGTG+CGG	+	chr4_3:10261904-10261923	None:intergenic	30.0%
ATGTGACTTAGAAACGAAAA+AGG	+	chr4_3:10262010-10262029	None:intergenic	30.0%
CGATTATGGTATGATAATCA+TGG	-	chr4_3:10262156-10262175	Msa0629400:intron	30.0%
TAGATATTGTGGAGTTGAAT+TGG	-	chr4_3:10262216-10262235	Msa0629400:intron	30.0%
GATCTATCGAATCAAAACAT+AGG	+	chr4_3:10262793-10262812	None:intergenic	30.0%
ATTAGTCGATTAAGACACAA+GGG	+	chr4_3:10263076-10263095	None:intergenic	30.0%
CACATTAACATTGTTGTCAT+TGG	-	chr4_3:10263213-10263232	Msa0629400:intron	30.0%
AGTTTCATCCTATATGAAGA+AGG	-	chr4_3:10263813-10263832	Msa0629400:intron	30.0%
TGTGATGTTTAAATCTTTGC+AGG	-	chr4_3:10264951-10264970	Msa0629400:five_prime_UTR	30.0%
GTGATGTTTAAATCTTTGCA+GGG	-	chr4_3:10264952-10264971	Msa0629400:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	10261888	10265025	10261888	ID=Msa0629400;Name=Msa0629400
chr4_3	mRNA	10261888	10265025	10261888	ID=Msa0629400-mRNA-1;Parent=Msa0629400;Name=Msa0629400-mRNA-1;_AED=0.07;_eAED=0.07;_QI=136\|1\|1\|1\|0.33\|0\|4\|311\|447
chr4_3	exon	10261888	10262164	10261888	ID=Msa0629400-mRNA-1:exon:3122;Parent=Msa0629400-mRNA-1
chr4_3	exon	10262241	10263156	10262241	ID=Msa0629400-mRNA-1:exon:3121;Parent=Msa0629400-mRNA-1
chr4_3	exon	10263257	10263749	10263257	ID=Msa0629400-mRNA-1:exon:3120;Parent=Msa0629400-mRNA-1
chr4_3	exon	10264921	10265025	10264921	ID=Msa0629400-mRNA-1:exon:3119;Parent=Msa0629400-mRNA-1
chr4_3	five_prime_UTR	10264921	10265025	10264921	ID=Msa0629400-mRNA-1:five_prime_utr;Parent=Msa0629400-mRNA-1
chr4_3	five_prime_UTR	10263719	10263749	10263719	ID=Msa0629400-mRNA-1:five_prime_utr;Parent=Msa0629400-mRNA-1
chr4_3	CDS	10263257	10263718	10263257	ID=Msa0629400-mRNA-1:cds;Parent=Msa0629400-mRNA-1
chr4_3	CDS	10262275	10263156	10262275	ID=Msa0629400-mRNA-1:cds;Parent=Msa0629400-mRNA-1
chr4_3	three_prime_UTR	10262241	10262274	10262241	ID=Msa0629400-mRNA-1:three_prime_utr;Parent=Msa0629400-mRNA-1
chr4_3	three_prime_UTR	10261888	10262164	10261888	ID=Msa0629400-mRNA-1:three_prime_utr;Parent=Msa0629400-mRNA-1

Gene Sequence

>Msa0629400

ATGGGTAAGGAAAAGGTTCACATTAACATTGTTGTCATTGGCCATGTCGACTCTGGGAAGTCAACTACCACTGGTCACTTGATCTACAAGCTCGGAGGTATTGACAAGCGTGTGATCGAGAGGTTTGAGAAAGAAGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCTTGGGTTCTTGACAAGCTCAAGGCTGAGCGTGAAAGAGGTATCACAATTGACATTGCTCTGTGGAAGTTTGAGACAACTAAGTACTACTGCACTGTCATTGATGCCCCCGGACACAGGGATTTCATTAAGAACATGATTACTGGTACATCCCAAGCTGATTGTGCTGTTCTCATCATTGACTCCACTACTGGTGGTTTTGAAGCTGGTATTTCCAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAGGCAAATGATCTGCTGCTGCAACAAGATGGATGCCACTACACCCAAGTATTCCAAGAGCAGGTATGATGAAATTGTGAAGGAAGTTTCATCCTATATGAAGAAGGTTGGCTATAACCCAGATAAAATTCCATTCGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCTACAAATCTTGACTGGTACAAGGGCCCAACACTTCTTGAGGCTCTTGATCAAATCAATGAGCCTAAGAGGCCATCAGACAAGCCCCTCCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTTCCTGTCGGACGTGTTGAGACTGGTACCATGAAACCTGGAATGGTGGTGACTTTTGCTCCAACTGGACTGCAAACTGAAGTCAAGTCTGTGGAGATGCATCATGAATCTCTCACTGAGGCACTTCCCGGTGACAATGTGGGATTTAATGTTAAGAATGTTTCTGTCAAGGATCTCAAGCGTGGTTATGTTGCCTCAAACACCAAGGATGACCCAGCCAAGGAGGCTGCTAACTTCACATCTCAAGTGATTATCATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTGCTTGACTGCCACACCTCCCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGACAGGCGTTCTGGTAAGGAGATCGAGAAGGAGCCCAAGTTCCTGAAGAATGGAGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTTGTTGAGACTTTCTCTGAATATCCTCCACTTGGCCGTTTTGCTGTCAGAGATATGCGTCAAACTGTGGCTGTTGGGGTCATCAAGGGTGTGGAGAAGAAGGAACCTGGCACTGTTAAGATAACTAAGTCAGCAGCCAAGAAGAAATGA

Protein sequence

>Msa0629400

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVRQMICCCNKMDATTPKYSKSRYDEIVKEVSSYMKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGTMKPGMVVTFAPTGLQTEVKSVEMHHESLTEALPGDNVGFNVKNVSVKDLKRGYVASNTKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKGVEKKEPGTVKITKSAAKKK*