AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0640660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640660 MtrunA17_Chr8g0389901 98.901 182 2 0 1 182 1 182 5.09e-135 374
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640660 AT5G15750.1 80.110 181 36 0 1 181 1 181 2.68e-110 312

Find 77 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATGGTTATCTCCTTTAA+TGG 0.197442 4_3:+39796108 Msa0640660:three_prime_UTR
ATACAGGGGTTAATGAAATT+TGG 0.202523 4_3:+39796166 Msa0640660:three_prime_UTR
GCTGGGTCTGTAATTGTATT+TGG 0.229997 4_3:-39795880 None:intergenic
ATTTCATTAACCCCTGTATA+TGG 0.259342 4_3:-39796162 None:intergenic
ACTAATTCTTCGAGCTGTAT+AGG 0.280666 4_3:+39796251 Msa0640660:three_prime_UTR
TGATATGACTGATAAGCTTT+TGG 0.287922 4_3:+39794344 Msa0640660:CDS
GTCTTCCCTCCGTCCCTCTA+AGG 0.341843 4_3:+39793767 Msa0640660:five_prime_UTR
GTATGGTTATCTCCTTTAAT+GGG 0.342327 4_3:+39796109 Msa0640660:three_prime_UTR
ACCAAGACTGTTTGAATTTG+TGG 0.357222 4_3:+39796070 Msa0640660:three_prime_UTR
AAAAGAATCATTCCATATAC+AGG 0.364481 4_3:+39796150 Msa0640660:three_prime_UTR
AGCTGTAACTTATATTGAAC+AGG 0.366283 4_3:+39795840 Msa0640660:CDS
CTAATTCTTCGAGCTGTATA+GGG 0.379975 4_3:+39796252 Msa0640660:three_prime_UTR
AAGCACCTTTCTCTTTATCT+TGG 0.381545 4_3:-39795953 None:intergenic
CTATGATTTAATGAATTAGT+TGG 0.383623 4_3:+39795999 Msa0640660:exon
ACGTAGGCTCTCAACTGTTT+TGG 0.388493 4_3:+39795786 Msa0640660:CDS
ACGGCAGCTGAAGGTTATCT+AGG 0.413061 4_3:-39793731 None:intergenic
TTTAATGACAGTTACAATAT+GGG 0.424343 4_3:+39794926 Msa0640660:intron
CTTCCATGTTTCTAGTTACA+AGG 0.426456 4_3:-39795906 None:intergenic
ATCAACTCGGAAAGGATCCT+TGG 0.429618 4_3:-39794325 None:intergenic
CTGAAGGTTATCTAGGGTTA+CGG 0.432215 4_3:-39793724 None:intergenic
TGAAGGTTATCTAGGGTTAC+GGG 0.439284 4_3:-39793723 None:intergenic
GGTTAATGAAATTTGGATGT+AGG 0.444873 4_3:+39796173 Msa0640660:three_prime_UTR
TTTCTAGTTACAAGGTAAGC+TGG 0.444914 4_3:-39795898 None:intergenic
ACATAGTGTGATGCTTTGCC+TGG 0.454332 4_3:-39794960 None:intergenic
TCCACAAATTCAAACAGTCT+TGG 0.459333 4_3:-39796071 None:intergenic
CGAGCTGTATAGGGCCGCAA+TGG 0.468684 4_3:+39796261 Msa0640660:three_prime_UTR
TAACCGTGTCAGCCTTCTGC+AGG 0.495522 4_3:+39794990 Msa0640660:CDS
ATGCCGGATGGTTCAGAAGT+TGG 0.502345 4_3:+39794278 Msa0640660:CDS
ATGCAGCGCTACCATGTTAC+CGG 0.507249 4_3:+39794104 Msa0640660:CDS
AGAACAAGAACGCACCTTAG+AGG 0.507562 4_3:-39793781 None:intergenic
GAGCTGTATAGGGCCGCAAT+GGG 0.510241 4_3:+39796262 Msa0640660:three_prime_UTR
AACCTTCAGCTGCCGTCTAC+CGG 0.517843 4_3:+39793738 Msa0640660:five_prime_UTR
GAAGACGGTGGGCCGGTAGA+CGG 0.519118 4_3:-39793750 None:intergenic
ACGGAGGGAAGACGGTGGGC+CGG 0.523624 4_3:-39793757 None:intergenic
TTCTAGTTACAAGGTAAGCT+GGG 0.525625 4_3:-39795897 None:intergenic
AGTGTGATGCTTTGCCTGGT+TGG 0.526362 4_3:-39794956 None:intergenic
TCAACTCGGAAAGGATCCTT+GGG 0.536281 4_3:-39794324 None:intergenic
GCGCTACCATGTTACCGGTC+GGG 0.546021 4_3:+39794109 Msa0640660:CDS
GAACAAGAACGCACCTTAGA+GGG 0.546604 4_3:-39793780 None:intergenic
AATCGTCCCGACCGGTAACA+TGG 0.550659 4_3:-39794115 None:intergenic
ACATGGAAGACATGATTACA+TGG 0.553951 4_3:+39795920 Msa0640660:CDS
TTACCAACTTCTGAACCATC+CGG 0.558041 4_3:-39794281 None:intergenic
GGCCGGTAGACGGCAGCTGA+AGG 0.561188 4_3:-39793740 None:intergenic
CGGCAGCTGAAGGTTATCTA+GGG 0.562741 4_3:-39793730 None:intergenic
TTCTTCCAAGATAAAGAGAA+AGG 0.564085 4_3:+39795948 Msa0640660:CDS
TTGGAATGGAAGAGAGAAGG+GGG 0.573733 4_3:+39794062 Msa0640660:CDS
TTAGAGGGACGGAGGGAAGA+CGG 0.583489 4_3:-39793765 None:intergenic
AAAGAATCATTCCATATACA+GGG 0.591714 4_3:+39796151 Msa0640660:three_prime_UTR
TTACCTGCAGAAGGCTGACA+CGG 0.596614 4_3:-39794993 None:intergenic
ATAGGTATTCGAGTATATGC+CGG 0.597856 4_3:+39794262 Msa0640660:intron
GAACAGGGACATGTTCGAGT+TGG 0.599994 4_3:+39795856 Msa0640660:CDS
AGGGTTACGGGATGGTATGT+CGG 0.601017 4_3:-39793711 None:intergenic
GGTAAACACGGTGAAACAGA+TGG 0.602021 4_3:+39794299 Msa0640660:CDS
AGGGACGGAGGGAAGACGGT+GGG 0.602483 4_3:-39793761 None:intergenic
GCTTCAGTACAATGACAAGT+TGG 0.608210 4_3:+39795972 Msa0640660:CDS
CTGTATAGGGCCGCAATGGG+TGG 0.619087 4_3:+39796265 Msa0640660:three_prime_UTR
GGAAGACATGATTACATGGG+TGG 0.620356 4_3:+39795924 Msa0640660:CDS
TCAGTCATATCAACTCGGAA+AGG 0.622552 4_3:-39794333 None:intergenic
AGCTAAAGCATCATGAGCAG+AGG 0.629474 4_3:+39794018 Msa0640660:CDS
GAGGGACGGAGGGAAGACGG+TGG 0.629608 4_3:-39793762 None:intergenic
AGCGCTACCATGTTACCGGT+CGG 0.636484 4_3:+39794108 Msa0640660:CDS
GGTTCAGAAGTTGGTAAACA+CGG 0.643580 4_3:+39794287 Msa0640660:CDS
TTTGGAATGGAAGAGAGAAG+GGG 0.646764 4_3:+39794061 Msa0640660:CDS
AACGCACCTTAGAGGGACGG+AGG 0.647446 4_3:-39793773 None:intergenic
GGTGAAACAGATGGATCCCA+AGG 0.652966 4_3:+39794308 Msa0640660:CDS
AAGAACGCACCTTAGAGGGA+CGG 0.653666 4_3:-39793776 None:intergenic
ACGCACCTTAGAGGGACGGA+GGG 0.655170 4_3:-39793772 None:intergenic
TTACCTTGTAACTAGAAACA+TGG 0.656200 4_3:+39795903 Msa0640660:CDS
ATATGGGTGTGATTCCAACC+AGG 0.661105 4_3:+39794942 Msa0640660:CDS
GCTTATCAGTCATATCAACT+CGG 0.663758 4_3:-39794338 None:intergenic
TAGGTCTTTACCACCCATTG+CGG 0.675427 4_3:-39796275 None:intergenic
GGTTATCTAGGGTTACGGGA+TGG 0.682855 4_3:-39793719 None:intergenic
GTATTCGAGTATATGCCGGA+TGG 0.685170 4_3:+39794266 Msa0640660:CDS
CATGGAAGACATGATTACAT+GGG 0.691863 4_3:+39795921 Msa0640660:CDS
AAGAATCATTCCATATACAG+GGG 0.707301 4_3:+39796152 Msa0640660:three_prime_UTR
GCTGTAACTTATATTGAACA+GGG 0.722848 4_3:+39795841 Msa0640660:CDS
GATGACAGTGAATAACAATG+AGG 0.742815 4_3:+39793994 Msa0640660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCAAATTATTTTAAGATA+AGG - chr4_3:39793946-39793965 None:intergenic 10.0%
!! TAATATTTGTTTCATAATTA+TGG + chr4_3:39794411-39794430 Msa0640660:intron 10.0%
!!! TTTTCATATGAAAAAAAATA+AGG + chr4_3:39795084-39795103 Msa0640660:intron 10.0%
!! TTTCATATGAAAAAAAATAA+GGG + chr4_3:39795085-39795104 Msa0640660:intron 10.0%
!! ACTAAATTTAATAGAAAAAT+AGG + chr4_3:39795473-39795492 Msa0640660:intron 10.0%
!!! CTTTTATTTTATTTTTTAAC+TGG + chr4_3:39796211-39796230 Msa0640660:three_prime_UTR 10.0%
!!! TTTATTTTATTTTTTAACTG+GGG + chr4_3:39796213-39796232 Msa0640660:three_prime_UTR 10.0%
!!! CTTTTCTCTTTTTTTATTAT+GGG + chr4_3:39793839-39793858 Msa0640660:intron 15.0%
!!! TATTTTTCTTTGTTTTAGTT+TGG + chr4_3:39794187-39794206 Msa0640660:intron 15.0%
!! TAAGTTATATAACAAAACTA+TGG - chr4_3:39794721-39794740 None:intergenic 15.0%
!! TTTATTACATTCACATAAAA+TGG - chr4_3:39795454-39795473 None:intergenic 15.0%
!!! GCTTTTCTCTTTTTTTATTA+TGG + chr4_3:39793838-39793857 Msa0640660:intron 20.0%
!! TAAATTATATACAAGTTGCA+GGG + chr4_3:39794584-39794603 Msa0640660:intron 20.0%
!!! TATTTTCATCACATTTCATT+TGG - chr4_3:39794654-39794673 None:intergenic 20.0%
!! AAATGAAATGTGATGAAAAT+AGG + chr4_3:39794653-39794672 Msa0640660:intron 20.0%
!! GTAACTGTCATTAAAAAAAA+AGG - chr4_3:39794921-39794940 None:intergenic 20.0%
!!! TTTTAATGACAGTTACAATA+TGG + chr4_3:39794925-39794944 Msa0640660:intron 20.0%
!! TTTAATGACAGTTACAATAT+GGG + chr4_3:39794926-39794945 Msa0640660:intron 20.0%
!! AGTTAATAAAGTTGAAACAT+TGG + chr4_3:39795636-39795655 Msa0640660:intron 20.0%
!! CTATGATTTAATGAATTAGT+TGG + chr4_3:39795999-39796018 Msa0640660:exon 20.0%
!! AAAAAAGAATTTCCCATTAA+AGG - chr4_3:39796124-39796143 None:intergenic 20.0%
! AATAAGCTAAAAACAGCTTA+TGG - chr4_3:39793880-39793899 None:intergenic 25.0%
! CATCACAATTCATTCTATTA+AGG - chr4_3:39793978-39793997 None:intergenic 25.0%
!!! TTTGAGAAAGGTGAATTTTT+TGG + chr4_3:39794043-39794062 Msa0640660:CDS 25.0%
!! TTTTATGTTGTTCTTGCATT+TGG + chr4_3:39794164-39794183 Msa0640660:intron 25.0%
!! ATTCATTTTCTTTGTGGAAT+TGG + chr4_3:39794220-39794239 Msa0640660:intron 25.0%
!! TTCATTTTCTTTGTGGAATT+GGG + chr4_3:39794221-39794240 Msa0640660:intron 25.0%
!! ATTGTGGTGGAAATATTAAT+AGG + chr4_3:39794244-39794263 Msa0640660:intron 25.0%
! AGATTATGTCAAAAGAAAGA+TGG - chr4_3:39794473-39794492 None:intergenic 25.0%
! GTAAATTATATACAAGTTGC+AGG + chr4_3:39794583-39794602 Msa0640660:intron 25.0%
! ATGACATAAATAGTGAATAC+AGG + chr4_3:39794625-39794644 Msa0640660:intron 25.0%
! TATAACTTACAAGTTTGTCT+AGG + chr4_3:39794732-39794751 Msa0640660:intron 25.0%
!! TTAGATTTGCCATTTCAAAA+AGG + chr4_3:39794773-39794792 Msa0640660:intron 25.0%
!!! AACAATCATCCTTTTTGAAA+TGG - chr4_3:39794785-39794804 None:intergenic 25.0%
!!! GAGGTATTAATGAACATTTT+TGG + chr4_3:39795183-39795202 Msa0640660:intron 25.0%
! GTGGTATATATATTGTAGTA+AGG + chr4_3:39795419-39795438 Msa0640660:intron 25.0%
!!! TGAAGAATTTCATTTTTCAG+TGG - chr4_3:39795503-39795522 None:intergenic 25.0%
!! TAAAGTTGAAACATTGGTTA+TGG + chr4_3:39795642-39795661 Msa0640660:intron 25.0%
!!! GATGAATTTTTGACTTTGTA+TGG + chr4_3:39796092-39796111 Msa0640660:three_prime_UTR 25.0%
! AAAAGAATCATTCCATATAC+AGG + chr4_3:39796150-39796169 Msa0640660:three_prime_UTR 25.0%
! AAAGAATCATTCCATATACA+GGG + chr4_3:39796151-39796170 Msa0640660:three_prime_UTR 25.0%
AATAGCAATAGAAAGGGAAA+GGG - chr4_3:39793814-39793833 None:intergenic 30.0%
TAATAGCAATAGAAAGGGAA+AGG - chr4_3:39793815-39793834 None:intergenic 30.0%
GCGAATAATAGCAATAGAAA+GGG - chr4_3:39793820-39793839 None:intergenic 30.0%
AGCGAATAATAGCAATAGAA+AGG - chr4_3:39793821-39793840 None:intergenic 30.0%
!!! GAAAGGTGAATTTTTTGGAA+TGG + chr4_3:39794048-39794067 Msa0640660:CDS 30.0%
!! TAGCTGATTCATTTTCTTTG+TGG + chr4_3:39794214-39794233 Msa0640660:intron 30.0%
! TGATATGACTGATAAGCTTT+TGG + chr4_3:39794344-39794363 Msa0640660:CDS 30.0%
ATAAGAAGCAAAAAGAAAGC+AGG - chr4_3:39794819-39794838 None:intergenic 30.0%
! GCTTTCTTTTTGCTTCTTAT+TGG + chr4_3:39794819-39794838 Msa0640660:intron 30.0%
TAATGAGCAAACAGATAAAG+TGG - chr4_3:39795057-39795076 None:intergenic 30.0%
TATGAAAAAAAATAAGGGCC+AGG + chr4_3:39795090-39795109 Msa0640660:intron 30.0%
!! CAAGAAAGTGTCTATAGTTA+TGG + chr4_3:39795152-39795171 Msa0640660:intron 30.0%
AGTGTCTATAGTTATGGATT+TGG + chr4_3:39795158-39795177 Msa0640660:intron 30.0%
GTGTCTATAGTTATGGATTT+GGG + chr4_3:39795159-39795178 Msa0640660:intron 30.0%
!!! CAGTTGTTTTAATACAGTTG+AGG - chr4_3:39795251-39795270 None:intergenic 30.0%
GTAATCATCTAACATTAGCA+TGG + chr4_3:39795273-39795292 Msa0640660:intron 30.0%
TTTATCATTGCTTTGTAGCT+AGG + chr4_3:39795372-39795391 Msa0640660:intron 30.0%
TATATATTGTAGTAAGGCCA+AGG + chr4_3:39795425-39795444 Msa0640660:intron 30.0%
ATATATTGTAGTAAGGCCAA+GGG + chr4_3:39795426-39795445 Msa0640660:intron 30.0%
TAATCAACTGATGTTGCAAA+AGG - chr4_3:39795539-39795558 None:intergenic 30.0%
TTATCTTTCTTCTAATGCGA+CGG + chr4_3:39795570-39795589 Msa0640660:intron 30.0%
!! TTTTTAGCCCAAAACTTAGT+TGG - chr4_3:39795596-39795615 None:intergenic 30.0%
!!! ACTAAGTTTTGGGCTAAAAA+TGG + chr4_3:39795596-39795615 Msa0640660:intron 30.0%
AACAAAATTCGGAGCAAATT+AGG - chr4_3:39795753-39795772 None:intergenic 30.0%
! GCATGATAGTGAACAAAATT+CGG - chr4_3:39795764-39795783 None:intergenic 30.0%
AGCTGTAACTTATATTGAAC+AGG + chr4_3:39795840-39795859 Msa0640660:CDS 30.0%
GCTGTAACTTATATTGAACA+GGG + chr4_3:39795841-39795860 Msa0640660:CDS 30.0%
TTACCTTGTAACTAGAAACA+TGG + chr4_3:39795903-39795922 Msa0640660:CDS 30.0%
TTCTTCCAAGATAAAGAGAA+AGG + chr4_3:39795948-39795967 Msa0640660:CDS 30.0%
TGTATGGTTATCTCCTTTAA+TGG + chr4_3:39796108-39796127 Msa0640660:three_prime_UTR 30.0%
GTATGGTTATCTCCTTTAAT+GGG + chr4_3:39796109-39796128 Msa0640660:three_prime_UTR 30.0%
AAGAATCATTCCATATACAG+GGG + chr4_3:39796152-39796171 Msa0640660:three_prime_UTR 30.0%
ATTTCATTAACCCCTGTATA+TGG - chr4_3:39796165-39796184 None:intergenic 30.0%
ATACAGGGGTTAATGAAATT+TGG + chr4_3:39796166-39796185 Msa0640660:three_prime_UTR 30.0%
GGTTAATGAAATTTGGATGT+AGG + chr4_3:39796173-39796192 Msa0640660:three_prime_UTR 30.0%
GATGACAGTGAATAACAATG+AGG + chr4_3:39793994-39794013 Msa0640660:intron 35.0%
!!! TTTTTGGAATGGAAGAGAGA+AGG + chr4_3:39794059-39794078 Msa0640660:CDS 35.0%
!!! TTTTGGAATGGAAGAGAGAA+GGG + chr4_3:39794060-39794079 Msa0640660:CDS 35.0%
ATAGGTATTCGAGTATATGC+CGG + chr4_3:39794262-39794281 Msa0640660:intron 35.0%
GCTTATCAGTCATATCAACT+CGG - chr4_3:39794341-39794360 None:intergenic 35.0%
! AGATACGACACTATGAGTTT+TGG + chr4_3:39794532-39794551 Msa0640660:intron 35.0%
ATTATATACAAGTTGCAGGG+TGG + chr4_3:39794587-39794606 Msa0640660:intron 35.0%
TATAGTTATGGATTTGGGTG+AGG + chr4_3:39795164-39795183 Msa0640660:intron 35.0%
!!! ATTTTTGGCCTTCAAAGTCT+AGG + chr4_3:39795198-39795217 Msa0640660:intron 35.0%
ATCTAACATTAGCATGGACA+TGG + chr4_3:39795279-39795298 Msa0640660:intron 35.0%
! TGCCAAGTTATCTAGTTTCA+AGG + chr4_3:39795317-39795336 Msa0640660:intron 35.0%
! GCCAAGTTATCTAGTTTCAA+GGG + chr4_3:39795318-39795337 Msa0640660:intron 35.0%
GTCACATGAAGGTTAACATA+TGG + chr4_3:39795349-39795368 Msa0640660:intron 35.0%
!! ATACCACTTTTCATACGACT+CGG - chr4_3:39795406-39795425 None:intergenic 35.0%
CAACTAACAAGACGAGATTA+GGG - chr4_3:39795676-39795695 None:intergenic 35.0%
ACAACTAACAAGACGAGATT+AGG - chr4_3:39795677-39795696 None:intergenic 35.0%
! TTCTAGTTACAAGGTAAGCT+GGG - chr4_3:39795900-39795919 None:intergenic 35.0%
!! TTTCTAGTTACAAGGTAAGC+TGG - chr4_3:39795901-39795920 None:intergenic 35.0%
!! CTTCCATGTTTCTAGTTACA+AGG - chr4_3:39795909-39795928 None:intergenic 35.0%
ACATGGAAGACATGATTACA+TGG + chr4_3:39795920-39795939 Msa0640660:CDS 35.0%
CATGGAAGACATGATTACAT+GGG + chr4_3:39795921-39795940 Msa0640660:CDS 35.0%
AAGCACCTTTCTCTTTATCT+TGG - chr4_3:39795956-39795975 None:intergenic 35.0%
ACCAAGACTGTTTGAATTTG+TGG + chr4_3:39796070-39796089 Msa0640660:three_prime_UTR 35.0%
TCCACAAATTCAAACAGTCT+TGG - chr4_3:39796074-39796093 None:intergenic 35.0%
ACTAATTCTTCGAGCTGTAT+AGG + chr4_3:39796251-39796270 Msa0640660:three_prime_UTR 35.0%
CTAATTCTTCGAGCTGTATA+GGG + chr4_3:39796252-39796271 Msa0640660:three_prime_UTR 35.0%
! TGAAGGTTATCTAGGGTTAC+GGG - chr4_3:39793726-39793745 None:intergenic 40.0%
! CTGAAGGTTATCTAGGGTTA+CGG - chr4_3:39793727-39793746 None:intergenic 40.0%
! TTTGGAATGGAAGAGAGAAG+GGG + chr4_3:39794061-39794080 Msa0640660:CDS 40.0%
TCTTTGTGGAATTGGGATTG+TGG + chr4_3:39794228-39794247 Msa0640660:intron 40.0%
TTACCAACTTCTGAACCATC+CGG - chr4_3:39794284-39794303 None:intergenic 40.0%
!! GGTTCAGAAGTTGGTAAACA+CGG + chr4_3:39794287-39794306 Msa0640660:CDS 40.0%
TCAGTCATATCAACTCGGAA+AGG - chr4_3:39794336-39794355 None:intergenic 40.0%
AATAGGTGAACTGACTGCAA+TGG + chr4_3:39794670-39794689 Msa0640660:intron 40.0%
AACTGACTGCAATGGTTCAT+TGG + chr4_3:39794678-39794697 Msa0640660:intron 40.0%
CTTGTCCTATTTAACTAGCC+TGG - chr4_3:39795111-39795130 None:intergenic 40.0%
AGACTGTTCCTAGACTTTGA+AGG - chr4_3:39795209-39795228 None:intergenic 40.0%
GCCCTTGAAACTAGATAACT+TGG - chr4_3:39795322-39795341 None:intergenic 40.0%
! GGGCAAATTTTGTCACATGA+AGG + chr4_3:39795338-39795357 Msa0640660:intron 40.0%
! TTTCATACGACTCGGAATAG+TGG - chr4_3:39795398-39795417 None:intergenic 40.0%
ATTCCGAGTCGTATGAAAAG+TGG + chr4_3:39795400-39795419 Msa0640660:intron 40.0%
TTCACATAAAATGGCACCCT+TGG - chr4_3:39795445-39795464 None:intergenic 40.0%
GACGATGTAATGCAAAGTTG+AGG - chr4_3:39795731-39795750 None:intergenic 40.0%
GCTGGGTCTGTAATTGTATT+TGG - chr4_3:39795883-39795902 None:intergenic 40.0%
GCTTCAGTACAATGACAAGT+TGG + chr4_3:39795972-39795991 Msa0640660:CDS 40.0%
GAACAAGAACGCACCTTAGA+GGG - chr4_3:39793783-39793802 None:intergenic 45.0%
AGAACAAGAACGCACCTTAG+AGG - chr4_3:39793784-39793803 None:intergenic 45.0%
! AGCTAAAGCATCATGAGCAG+AGG + chr4_3:39794018-39794037 Msa0640660:CDS 45.0%
!! TGAGCAGAGGCTTTTGAGAA+AGG + chr4_3:39794031-39794050 Msa0640660:CDS 45.0%
TTGGAATGGAAGAGAGAAGG+GGG + chr4_3:39794062-39794081 Msa0640660:CDS 45.0%
! CTTTTTGTAATCGTCCCGAC+CGG - chr4_3:39794126-39794145 None:intergenic 45.0%
TTGTGGAATTGGGATTGTGG+TGG + chr4_3:39794231-39794250 Msa0640660:intron 45.0%
GTATTCGAGTATATGCCGGA+TGG + chr4_3:39794266-39794285 Msa0640660:CDS 45.0%
GGTAAACACGGTGAAACAGA+TGG + chr4_3:39794299-39794318 Msa0640660:CDS 45.0%
TCAACTCGGAAAGGATCCTT+GGG - chr4_3:39794327-39794346 None:intergenic 45.0%
ATCAACTCGGAAAGGATCCT+TGG - chr4_3:39794328-39794347 None:intergenic 45.0%
ATATGGGTGTGATTCCAACC+AGG + chr4_3:39794942-39794961 Msa0640660:CDS 45.0%
ACATAGTGTGATGCTTTGCC+TGG - chr4_3:39794963-39794982 None:intergenic 45.0%
! GTGTTCCTTTTACCTGCAGA+AGG - chr4_3:39795005-39795024 None:intergenic 45.0%
! AAGGGCCAGGCTAGTTAAAT+AGG + chr4_3:39795103-39795122 Msa0640660:intron 45.0%
GTTCACTATCATGCAGACGT+AGG + chr4_3:39795770-39795789 Msa0640660:intron 45.0%
! ACGTAGGCTCTCAACTGTTT+TGG + chr4_3:39795786-39795805 Msa0640660:CDS 45.0%
GGAAGACATGATTACATGGG+TGG + chr4_3:39795924-39795943 Msa0640660:CDS 45.0%
TAGGTCTTTACCACCCATTG+CGG - chr4_3:39796278-39796297 None:intergenic 45.0%
!!! TTTTATTTTATTTTTTAACT+GGG + chr4_3:39796212-39796231 Msa0640660:three_prime_UTR 5.0%
AGGGTTACGGGATGGTATGT+CGG - chr4_3:39793714-39793733 None:intergenic 50.0%
! GGTTATCTAGGGTTACGGGA+TGG - chr4_3:39793722-39793741 None:intergenic 50.0%
CGGCAGCTGAAGGTTATCTA+GGG - chr4_3:39793733-39793752 None:intergenic 50.0%
ACGGCAGCTGAAGGTTATCT+AGG - chr4_3:39793734-39793753 None:intergenic 50.0%
AAGAACGCACCTTAGAGGGA+CGG - chr4_3:39793779-39793798 None:intergenic 50.0%
ATGCAGCGCTACCATGTTAC+CGG + chr4_3:39794104-39794123 Msa0640660:CDS 50.0%
ATGCCGGATGGTTCAGAAGT+TGG + chr4_3:39794278-39794297 Msa0640660:CDS 50.0%
GGTGAAACAGATGGATCCCA+AGG + chr4_3:39794308-39794327 Msa0640660:CDS 50.0%
AGTGTGATGCTTTGCCTGGT+TGG - chr4_3:39794959-39794978 None:intergenic 50.0%
TTACCTGCAGAAGGCTGACA+CGG - chr4_3:39794996-39795015 None:intergenic 50.0%
GTCAGCCTTCTGCAGGTAAA+AGG + chr4_3:39794997-39795016 Msa0640660:intron 50.0%
! TGCGACGGCCAACTAAGTTT+TGG + chr4_3:39795585-39795604 Msa0640660:intron 50.0%
! GCGACGGCCAACTAAGTTTT+GGG + chr4_3:39795586-39795605 Msa0640660:intron 50.0%
GAACAGGGACATGTTCGAGT+TGG + chr4_3:39795856-39795875 Msa0640660:CDS 50.0%
AACCTTCAGCTGCCGTCTAC+CGG + chr4_3:39793738-39793757 Msa0640660:five_prime_UTR 55.0%
TTAGAGGGACGGAGGGAAGA+CGG - chr4_3:39793768-39793787 None:intergenic 55.0%
AGGGGGTCACAGAGAGAACA+TGG + chr4_3:39794079-39794098 Msa0640660:CDS 55.0%
AGCGCTACCATGTTACCGGT+CGG + chr4_3:39794108-39794127 Msa0640660:CDS 55.0%
AATCGTCCCGACCGGTAACA+TGG - chr4_3:39794118-39794137 None:intergenic 55.0%
TAACCGTGTCAGCCTTCTGC+AGG + chr4_3:39794990-39795009 Msa0640660:CDS 55.0%
GAGCTGTATAGGGCCGCAAT+GGG + chr4_3:39796262-39796281 Msa0640660:three_prime_UTR 55.0%
GTCTTCCCTCCGTCCCTCTA+AGG + chr4_3:39793767-39793786 Msa0640660:five_prime_UTR 60.0%
ACGCACCTTAGAGGGACGGA+GGG - chr4_3:39793775-39793794 None:intergenic 60.0%
AACGCACCTTAGAGGGACGG+AGG - chr4_3:39793776-39793795 None:intergenic 60.0%
GCGCTACCATGTTACCGGTC+GGG + chr4_3:39794109-39794128 Msa0640660:CDS 60.0%
CGAGCTGTATAGGGCCGCAA+TGG + chr4_3:39796261-39796280 Msa0640660:three_prime_UTR 60.0%
CTGTATAGGGCCGCAATGGG+TGG + chr4_3:39796265-39796284 Msa0640660:three_prime_UTR 60.0%
GAAGACGGTGGGCCGGTAGA+CGG - chr4_3:39793753-39793772 None:intergenic 65.0%
AGGGACGGAGGGAAGACGGT+GGG - chr4_3:39793764-39793783 None:intergenic 65.0%
GGCCGGTAGACGGCAGCTGA+AGG - chr4_3:39793743-39793762 None:intergenic 70.0%
ACGGAGGGAAGACGGTGGGC+CGG - chr4_3:39793760-39793779 None:intergenic 70.0%
GAGGGACGGAGGGAAGACGG+TGG - chr4_3:39793765-39793784 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 39793691 39796299 39793691 ID=Msa0640660;Name=Msa0640660
chr4_3 mRNA 39793691 39796299 39793691 ID=Msa0640660-mRNA-1;Parent=Msa0640660;Name=Msa0640660-mRNA-1;_AED=0.04;_eAED=0.04;_QI=107|1|1|1|0.5|0.4|5|282|182
chr4_3 exon 39793691 39793788 39793691 ID=Msa0640660-mRNA-1:exon:8937;Parent=Msa0640660-mRNA-1
chr4_3 exon 39794002 39794144 39794002 ID=Msa0640660-mRNA-1:exon:8938;Parent=Msa0640660-mRNA-1
chr4_3 exon 39794266 39794373 39794266 ID=Msa0640660-mRNA-1:exon:8939;Parent=Msa0640660-mRNA-1
chr4_3 exon 39794937 39795011 39794937 ID=Msa0640660-mRNA-1:exon:8940;Parent=Msa0640660-mRNA-1
chr4_3 exon 39795786 39796299 39795786 ID=Msa0640660-mRNA-1:exon:8941;Parent=Msa0640660-mRNA-1
chr4_3 five_prime_UTR 39793691 39793788 39793691 ID=Msa0640660-mRNA-1:five_prime_utr;Parent=Msa0640660-mRNA-1
chr4_3 five_prime_UTR 39794002 39794010 39794002 ID=Msa0640660-mRNA-1:five_prime_utr;Parent=Msa0640660-mRNA-1
chr4_3 CDS 39794011 39794144 39794011 ID=Msa0640660-mRNA-1:cds;Parent=Msa0640660-mRNA-1
chr4_3 CDS 39794266 39794373 39794266 ID=Msa0640660-mRNA-1:cds;Parent=Msa0640660-mRNA-1
chr4_3 CDS 39794937 39795011 39794937 ID=Msa0640660-mRNA-1:cds;Parent=Msa0640660-mRNA-1
chr4_3 CDS 39795786 39796017 39795786 ID=Msa0640660-mRNA-1:cds;Parent=Msa0640660-mRNA-1
chr4_3 three_prime_UTR 39796018 39796299 39796018 ID=Msa0640660-mRNA-1:three_prime_utr;Parent=Msa0640660-mRNA-1
Gene Sequence

>Msa0640660

ATGAGGAAGCTAAAGCATCATGAGCAGAGGCTTTTGAGAAAGGTGAATTTTTTGGAATGGAAGAGAGAAGGGGGTCACAGAGAGAACATGGTTATGCAGCGCTACCATGTTACCGGTCGGGACGATTACAAAAAGTATTCGAGTATATGCCGGATGGTTCAGAAGTTGGTAAACACGGTGAAACAGATGGATCCCAAGGATCCTTTCCGAGTTGATATGACTGATAAGCTTTTGGAAAAACTTTACAATATGGGTGTGATTCCAACCAGGCAAAGCATCACACTATGTGAACGCTTAACCGTGTCAGCCTTCTGCAGACGTAGGCTCTCAACTGTTTTGGTGAAACTAAAATTTGCTGAGCATTTGAAAGAAGCTGTAACTTATATTGAACAGGGACATGTTCGAGTTGGTCCAAATACAATTACAGACCCAGCTTACCTTGTAACTAGAAACATGGAAGACATGATTACATGGGTGGATTCTTCCAAGATAAAGAGAAAGGTGCTTCAGTACAATGACAAGTTGGATGACTATGATTTAATGAATTAG
Protein sequence

>Msa0640660

MRKLKHHEQRLLRKVNFLEWKREGGHRENMVMQRYHVTGRDDYKKYSSICRMVQKLVNTVKQMDPKDPFRVDMTDKLLEKLYNMGVIPTRQSITLCERLTVSAFCRRRLSTVLVKLKFAEHLKEAVTYIEQGHVRVGPNTITDPAYLVTRNMEDMITWVDSSKIKRKVLQYNDKLDDYDLMN*