AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662830 MtrunA17_Chr8g0346021 99.275 552 4 0 1 552 1 552 0.0 1135
Msa0662830 MtrunA17_Chr6g0462191 34.805 385 179 16 112 486 89 411 2.85e-36 140
Msa0662830 MtrunA17_Chr1g0181581 28.906 384 215 9 112 486 101 435 1.51e-34 135
Msa0662830 MtrunA17_Chr7g0263731 31.486 397 209 13 107 486 88 438 1.78e-34 135
Msa0662830 MtrunA17_Chr4g0025441 45.545 202 89 7 126 323 95 279 2.55e-34 134
Msa0662830 MtrunA17_Chr1g0200161 38.860 193 100 4 126 314 140 318 4.46e-33 132
Msa0662830 MtrunA17_Chr4g0045641 27.652 528 283 18 1 485 1 472 2.49e-32 130
Msa0662830 MtrunA17_Chr3g0126901 35.754 358 165 10 1 333 1 318 3.76e-32 128
Msa0662830 MtrunA17_Chr1g0146931 43.961 207 97 6 116 305 111 315 1.37e-28 119
Msa0662830 MtrunA17_Chr6g0472551 30.411 365 169 11 129 477 119 414 7.95e-27 113
Msa0662830 MtrunA17_Chr3g0135851 40.933 193 88 4 124 305 88 265 8.46e-27 113
Msa0662830 MtrunA17_Chr4g0020671 29.936 314 149 13 1 300 1 257 6.09e-25 107
Msa0662830 MtrunA17_Chr4g0057321 28.090 445 226 11 30 467 15 372 7.83e-25 107
Msa0662830 MtrunA17_Chr1g0204901 47.531 162 72 4 45 197 48 205 6.42e-24 104
Msa0662830 MtrunA17_Chr1g0203711 31.250 352 156 12 126 466 114 390 1.70e-22 100
Msa0662830 MtrunA17_Chr8g0340761 45.026 191 82 4 44 226 36 211 1.90e-22 99.8
Msa0662830 MtrunA17_Chr2g0283321 32.787 183 103 4 126 305 62 227 5.66e-22 96.7
Msa0662830 MtrunA17_Chr8g0342551 39.474 190 89 4 125 305 88 260 1.60e-20 94.4
Msa0662830 MtrunA17_Chr8g0389781 73.333 60 16 0 126 185 125 184 4.09e-20 93.2
Msa0662830 MtrunA17_Chr1g0166431 44.286 140 56 4 45 179 47 169 1.94e-19 91.3
Msa0662830 MtrunA17_Chr7g0273861 31.148 183 107 4 118 300 116 279 1.76e-18 88.2
Msa0662830 MtrunA17_Chr7g0273951 39.726 146 62 3 47 191 50 170 5.89e-18 85.9
Msa0662830 MtrunA17_Chr8g0371131 60.656 61 24 0 125 185 69 129 2.95e-17 79.0
Msa0662830 MtrunA17_Chr4g0060381 61.905 63 24 0 129 191 102 164 3.75e-17 84.7
Msa0662830 MtrunA17_Chr2g0330921 60.000 70 28 0 124 193 94 163 1.01e-16 82.0
Msa0662830 MtrunA17_Chr2g0330051 39.500 200 77 8 125 303 76 252 4.17e-16 80.5
Msa0662830 MtrunA17_Chr2g0316401 28.934 197 114 8 127 316 74 251 8.27e-16 78.6
Msa0662830 MtrunA17_Chr1g0156121 57.895 57 24 0 129 185 126 182 1.30e-13 73.9
Msa0662830 MtrunA17_Chr7g0215371 52.727 55 26 0 129 183 21 75 4.22e-11 59.7
Msa0662830 MtrunA17_Chr5g0416161 33.846 130 70 4 60 183 56 175 4.26e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662830 AT1G01110.2 57.913 575 171 23 1 552 1 527 0.0 526
Msa0662830 AT4G00820.1 58.007 562 180 18 9 552 11 534 1.62e-180 519
Msa0662830 AT1G01110.1 59.446 397 114 16 170 552 1 364 2.62e-137 402
Msa0662830 AT4G10640.1 36.219 566 204 19 1 552 1 423 2.85e-89 281
Msa0662830 AT3G49380.1 37.959 245 113 5 1 239 1 212 2.83e-37 141
Msa0662830 AT2G26180.1 43.627 204 89 4 119 311 76 264 2.90e-35 137
Msa0662830 AT3G49260.1 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa0662830 AT3G49260.4 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa0662830 AT3G49260.2 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa0662830 AT5G03040.1 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa0662830 AT5G03040.3 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa0662830 AT5G03040.2 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa0662830 AT3G49260.3 34.501 371 181 14 1 331 1 349 2.89e-34 135
Msa0662830 AT3G22190.3 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa0662830 AT3G22190.2 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa0662830 AT3G22190.4 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa0662830 AT3G22190.1 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa0662830 AT1G72670.1 37.500 208 104 6 107 305 73 263 1.95e-29 120
Msa0662830 AT3G52290.1 33.228 316 155 13 1 304 1 272 8.87e-29 119
Msa0662830 AT3G59690.2 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa0662830 AT3G59690.1 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa0662830 AT2G43680.4 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa0662830 AT2G43680.2 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa0662830 AT2G43680.3 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa0662830 AT2G43680.5 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa0662830 AT2G43680.1 26.619 417 225 14 126 539 322 660 2.18e-25 111
Msa0662830 AT5G13460.1 29.710 276 153 7 38 300 18 265 4.43e-23 102
Msa0662830 AT4G14750.1 68.182 66 21 0 126 191 104 169 4.45e-22 99.0
Msa0662830 AT1G17480.1 36.842 171 81 4 100 270 68 211 9.18e-22 97.8
Msa0662830 AT4G14750.3 68.182 66 21 0 126 191 163 228 1.04e-21 98.6
Msa0662830 AT4G14750.2 68.182 66 21 0 126 191 163 228 1.09e-21 98.6
Msa0662830 AT3G09710.2 30.732 205 118 7 111 303 95 287 1.35e-21 98.2
Msa0662830 AT3G09710.1 30.653 199 112 7 111 303 95 273 1.73e-21 97.8
Msa0662830 AT2G26410.2 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa0662830 AT2G26410.1 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa0662830 AT3G16490.1 65.714 70 24 0 121 190 101 170 1.79e-19 91.3
Msa0662830 AT4G23060.1 28.255 361 165 11 114 467 152 425 9.90e-19 89.7
Msa0662830 AT4G29150.1 44.262 122 57 2 122 241 126 238 2.19e-18 87.8
Msa0662830 AT5G07240.1 67.742 62 20 0 124 185 103 164 5.83e-18 86.7
Msa0662830 AT5G07240.2 67.742 62 20 0 124 185 149 210 7.49e-18 86.7
Msa0662830 AT5G62070.1 70.690 58 17 0 124 181 114 171 7.25e-17 83.2
Msa0662830 AT1G18840.1 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa0662830 AT1G18840.3 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa0662830 AT1G18840.2 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa0662830 AT1G19870.1 35.556 135 70 2 71 205 173 290 7.50e-16 81.6
Msa0662830 AT2G33990.1 26.601 203 129 4 126 326 46 230 1.58e-15 77.4
Msa0662830 AT1G51960.1 60.000 65 26 0 126 190 99 163 1.93e-15 78.6
Msa0662830 AT1G19870.2 53.333 75 32 1 131 205 36 107 3.36e-15 79.0
Msa0662830 AT3G15050.1 26.630 184 115 4 129 308 52 219 1.33e-14 74.3
Msa0662830 AT1G74690.1 55.385 65 29 0 127 191 113 177 2.72e-14 76.3
Msa0662830 AT3G51380.1 48.235 85 43 1 108 192 19 102 3.14e-14 69.3
Msa0662830 AT1G14380.1 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa0662830 AT1G14380.3 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa0662830 AT1G14380.4 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa0662830 AT1G14380.6 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa0662830 AT1G14380.5 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa0662830 AT1G14380.2 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa0662830 AT1G14380.7 50.769 65 32 0 129 193 96 160 1.56e-13 73.9

Find 175 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATAACATGTAATATTT+TGG 0.059916 4_4:+682260 Msa0662830:three_prime_UTR
TTTACATAATAATAAAATTT+TGG 0.148736 4_4:+678250 Msa0662830:five_prime_UTR
GTGACAAGACAGTCCATTTA+AGG 0.176423 4_4:-682240 None:intergenic
GAAATTATGGACATAGTTTA+AGG 0.213922 4_4:+681708 Msa0662830:CDS
TTGTTCCACCCCTACTATTT+TGG 0.218545 4_4:-681472 None:intergenic
ATCCATCTATCTAGCCATTT+TGG 0.221443 4_4:-681083 None:intergenic
ATCTAATTGTGCAAAGCTTA+TGG 0.234585 4_4:-678224 None:intergenic
TGTGGATTATATTGATAATT+TGG 0.238180 4_4:-681230 None:intergenic
AAGAAACACTTGTGGATCTT+TGG 0.252655 4_4:-678652 None:intergenic
ACAACAAGTATGTCCTTAAA+TGG 0.267281 4_4:+682227 Msa0662830:three_prime_UTR
AGTACAACAAGAAGAGTAAT+TGG 0.281665 4_4:-681789 None:intergenic
CCAACTCCTCCTACATTATA+AGG 0.303150 4_4:-681680 None:intergenic
GGAGTTGGTTATGGAAATTA+TGG 0.312677 4_4:+681695 Msa0662830:CDS
TAGTGAGAGGTCACAATGTT+AGG 0.313052 4_4:+679839 Msa0662830:CDS
TAGTGACACTGCTAGTGTTT+GGG 0.318138 4_4:+679975 Msa0662830:CDS
AAGAGCACTTCGTGCGCTTA+AGG 0.319760 4_4:+679795 Msa0662830:CDS
TGATTTATGATTCAATATAA+TGG 0.328529 4_4:-678339 None:intergenic
CTCCATAGATCACACCAAAA+TGG 0.329388 4_4:+681287 Msa0662830:CDS
GGTTGTGAACGGATCCATTT+TGG 0.332251 4_4:-681301 None:intergenic
TACAAAACCATGGGAGAATA+GGG 0.336323 4_4:+681109 Msa0662830:CDS
GAGAACCATGTATTGTGTTT+TGG 0.353766 4_4:+682117 Msa0662830:three_prime_UTR
TGCAACGACTGCGGAGTATT+CGG 0.354857 4_4:+681907 Msa0662830:three_prime_UTR
GAAGTAGTATTGCAGATGAT+TGG 0.362742 4_4:+680259 Msa0662830:CDS
CTCATCTCAACCACCGTCTA+AGG 0.364361 4_4:-681853 None:intergenic
TACACATTCAACAATGTTAC+AGG 0.365782 4_4:-678602 None:intergenic
GGTTACAACTATGACACATT+AGG 0.366301 4_4:+681928 Msa0662830:three_prime_UTR
CTACAAAACCATGGGAGAAT+AGG 0.367491 4_4:+681108 Msa0662830:CDS
ACATCATGCTTCAACTTTGT+TGG 0.375678 4_4:-679176 None:intergenic
TTTGCTCCTAGATATGAATA+AGG 0.377417 4_4:-681191 None:intergenic
TCTCAACCTTATTCATATCT+AGG 0.385222 4_4:+681185 Msa0662830:CDS
GGAACTTGTACCAAAATAGT+AGG 0.389102 4_4:+681462 Msa0662830:CDS
TTAGTGACACTGCTAGTGTT+TGG 0.389305 4_4:+679974 Msa0662830:CDS
TCGGTTGCTTGCATGTAATT+CGG 0.404052 4_4:-681545 None:intergenic
TGTGAGTGAGTGAGAGGAGG+AGG 0.410940 4_4:-678295 None:intergenic
AATGTAGGAGGAGTTGGTTA+TGG 0.413219 4_4:+681686 Msa0662830:CDS
ATGCTACAAGTGTGGCTTCA+AGG 0.418682 4_4:+679204 Msa0662830:CDS
GAGGTTTCACCTTCGTCAAC+CGG 0.431189 4_4:+681827 Msa0662830:CDS
TTCTTTCCTTTGTTGCAACA+TGG 0.431704 4_4:-680316 None:intergenic
ATTATTACATTGCAAACAAA+AGG 0.433968 4_4:-682300 None:intergenic
CTTGACGACCGGACTTGAAT+AGG 0.434489 4_4:-681359 None:intergenic
ATGTTCTGGCCTACCTCACT+TGG 0.436846 4_4:+682184 Msa0662830:three_prime_UTR
CCTCTCAAACACCAAGCTTA+AGG 0.437895 4_4:+681420 Msa0662830:CDS
GGTGTCGATGGCCTTTGCCT+TGG 0.439851 4_4:-681602 None:intergenic
GTGAGGTAGGCCAGAACATA+TGG 0.440826 4_4:-682180 None:intergenic
GACCAAAATGGCTAGATAGA+TGG 0.442192 4_4:+681081 Msa0662830:CDS
TTCACTGTTTCATGATTCAC+TGG 0.442484 4_4:-679134 None:intergenic
CAGAAAGGTTCAACTTGGTT+GGG 0.443221 4_4:-678721 None:intergenic
CCTCCTACATTATAAGGATC+AGG 0.445401 4_4:-681674 None:intergenic
CATATGGCCAGCATTTATCT+TGG 0.445786 4_4:-682164 None:intergenic
AAGTAGTATTGCAGATGATT+GGG 0.451725 4_4:+680260 Msa0662830:CDS
GGTACAAGTTCCCATTGTAA+TGG 0.452470 4_4:-681451 None:intergenic
TGCCTTTGTGGTTGGTGATG+TGG 0.455595 4_4:-681248 None:intergenic
AAACCTGTTGTGAAAATGCT+TGG 0.456509 4_4:-680368 None:intergenic
CCTTATAATGTAGGAGGAGT+TGG 0.456681 4_4:+681680 Msa0662830:CDS
TTCATTTATTTGCACAAATG+AGG 0.457015 4_4:+678571 Msa0662830:five_prime_UTR
TAAATGCTGGCCATATGTTC+TGG 0.460943 4_4:+682170 Msa0662830:three_prime_UTR
GGTCTAATTACCATTACAAT+GGG 0.460991 4_4:+681441 Msa0662830:CDS
ACATTCACACTCTTGAAACT+TGG 0.462543 4_4:-681734 None:intergenic
CTGCTAGTGTTTGGGATTCG+CGG 0.462678 4_4:+679983 Msa0662830:CDS
AGAGCACTTCGTGCGCTTAA+GGG 0.465270 4_4:+679796 Msa0662830:CDS
GAGTGAGTGAGAGGAGGAGG+GGG 0.466047 4_4:-678292 None:intergenic
GGAAGAAAGGTAGTAGTTCA+TGG 0.469859 4_4:+678839 Msa0662830:CDS
AATTCGAGCCTTTGCAGACT+CGG 0.472168 4_4:-681564 None:intergenic
ACAGAAAGGTTCAACTTGGT+TGG 0.472834 4_4:-678722 None:intergenic
TCAAGAAGAAGATGAAGAAA+AGG 0.473802 4_4:+678942 Msa0662830:CDS
GTGAGTGAGTGAGAGGAGGA+GGG 0.475121 4_4:-678294 None:intergenic
ACACACAGTTGAAGAAGTTA+AGG 0.475450 4_4:+680293 Msa0662830:CDS
ATTTCACAGATTTGGAGAAA+TGG 0.475842 4_4:+681017 Msa0662830:intron
AATGGTAGGACATCATCAAT+TGG 0.477140 4_4:+681035 Msa0662830:CDS
GGAAAGAAGCAGCAATGAAG+AGG 0.480771 4_4:+680331 Msa0662830:CDS
CGCAGAGTGCGCCAAGGCAA+AGG 0.484032 4_4:+681591 Msa0662830:CDS
CACAACGAGGGCTTGACGAC+CGG 0.488125 4_4:-681370 None:intergenic
TGTGGCTTCAAGGACAGATC+AGG 0.489395 4_4:+679214 Msa0662830:CDS
ACATTATAAGGATCAGGAGC+AGG 0.491564 4_4:-681668 None:intergenic
AATGTAGCAGCATCACATTA+TGG 0.491586 4_4:+681752 Msa0662830:CDS
TGTTCTGGCCTACCTCACTT+GGG 0.492874 4_4:+682185 Msa0662830:three_prime_UTR
CGTCAACCGGTGACCTTAGA+CGG 0.494779 4_4:+681840 Msa0662830:CDS
CTATCCCTCTCTGGTGTCGA+TGG 0.494815 4_4:-681614 None:intergenic
TGAGTGAGTGAGAGGAGGAG+GGG 0.494935 4_4:-678293 None:intergenic
TAAAGTCTTTAAAGAAGAGA+AGG 0.495381 4_4:-678695 None:intergenic
GAACTTGTACCAAAATAGTA+GGG 0.496333 4_4:+681463 Msa0662830:CDS
GTGAAGTAGACCTTATCCTT+TGG 0.496611 4_4:-679927 None:intergenic
GATCCGGATCTATCCCTCTC+TGG 0.498338 4_4:-681623 None:intergenic
GATCCGGATCAGTAAAGAAA+AGG 0.499138 4_4:+681636 Msa0662830:CDS
GCTCTTCCCCTATTCTCCCA+TGG 0.500010 4_4:-681116 None:intergenic
ACATGCTCTTGCTGTGGCTA+TGG 0.501053 4_4:+679244 Msa0662830:CDS
CTAAGAGTTCAAATCATGCT+AGG 0.504978 4_4:+679324 Msa0662830:CDS
AGTCTGCAAAGGCTCGAATT+CGG 0.506521 4_4:+681567 Msa0662830:CDS
TAAGGACATACTTGTTGTGT+AGG 0.509479 4_4:-682222 None:intergenic
TTGCAGTGTTGAAGACAGAA+AGG 0.511229 4_4:-678736 None:intergenic
AACTTGAAGAGAGACCAAAA+TGG 0.518980 4_4:+681069 Msa0662830:CDS
TGCTGGAGACGGAGTAGTGA+TGG 0.522782 4_4:-681327 None:intergenic
GCTCCTGATCCTTATAATGT+AGG 0.524729 4_4:+681671 Msa0662830:CDS
CCTTAAGCTTGGTGTTTGAG+AGG 0.526162 4_4:-681420 None:intergenic
GAAGCAATGGAATGCCTTTG+TGG 0.526434 4_4:-681260 None:intergenic
AACCTGTTGTGAAAATGCTT+GGG 0.527631 4_4:-680367 None:intergenic
AGGTCTAATTACCATTACAA+TGG 0.529005 4_4:+681440 Msa0662830:CDS
GAGTTCTTGACCAAAGGATA+AGG 0.529710 4_4:+679917 Msa0662830:CDS
ATCTTCAAGACATTTCAGAT+AGG 0.531548 4_4:+680007 Msa0662830:CDS
AGCTACTGCTCAAGCTGCTG+TGG 0.535167 4_4:+679286 Msa0662830:CDS
GCCTAAATAAAATAAGTGAA+TGG 0.536968 4_4:-681961 None:intergenic
TCACAAAGAAACAAACAGTA+AGG 0.537387 4_4:+678468 Msa0662830:five_prime_UTR
ATATAATGGTAGTCAGAGAC+AGG 0.538519 4_4:-678325 None:intergenic
AAAGAAAAGAAGTTAGCATA+GGG 0.539161 4_4:+682033 Msa0662830:three_prime_UTR
AGTGTCAGAAGGTATACCTT+AGG 0.542037 4_4:-678169 None:intergenic
AAAAGAAAAGAAGTTAGCAT+AGG 0.544970 4_4:+682032 Msa0662830:three_prime_UTR
GAGCACTTCGTGCGCTTAAG+GGG 0.545080 4_4:+679797 Msa0662830:CDS
AGCAGCATCACATTATGGAT+TGG 0.546365 4_4:+681757 Msa0662830:CDS
TCTTGTTGTACTGAGAGTCT+CGG 0.552055 4_4:+681800 Msa0662830:CDS
GGAGCATTATGCTGCTGTTG+TGG 0.552104 4_4:+679346 Msa0662830:CDS
AGCTTGGTGTTTGAGAGGTG+TGG 0.552600 4_4:-681415 None:intergenic
TTAACTTCTTCAACTGTGTG+TGG 0.553478 4_4:-680291 None:intergenic
GCAAGTACTATGTTGTTGCT+AGG 0.553496 4_4:+682089 Msa0662830:three_prime_UTR
AACACTAGCAGTGTCACTAA+AGG 0.555204 4_4:-679971 None:intergenic
AATCAAGACCTATTCAAGTC+CGG 0.556812 4_4:+681351 Msa0662830:CDS
TACTGAGAGTCTCGGTGGCG+AGG 0.556933 4_4:+681808 Msa0662830:CDS
ACACCAGAGAGGGATAGATC+CGG 0.558129 4_4:+681620 Msa0662830:CDS
GAAAGAAGCAGCAATGAAGA+GGG 0.561558 4_4:+680332 Msa0662830:CDS
AGTAGGGGTGGAACAAGTAA+TGG 0.561747 4_4:+681479 Msa0662830:CDS
TTAAGGCCATGTTGCAACAA+AGG 0.561941 4_4:+680310 Msa0662830:CDS
GGATGATCAAAGAAAGTGAA+AGG 0.565239 4_4:-678130 None:intergenic
TGTGTTTGTGAGTGAGTGAG+AGG 0.565316 4_4:-678301 None:intergenic
GAAGACAGAAAGGTTCAACT+TGG 0.566144 4_4:-678726 None:intergenic
CACAGATTTGGAGAAATGGT+AGG 0.567014 4_4:+681021 Msa0662830:intron
TCAACTACACTCAGTTTCAA+CGG 0.568119 4_4:+681881 Msa0662830:three_prime_UTR
TAGGATGCCAAGATAAATGC+TGG 0.570025 4_4:+682157 Msa0662830:three_prime_UTR
TCTATGGAGCGGTGAAGCAA+TGG 0.570311 4_4:-681273 None:intergenic
TAAAACCAAAACACAATACA+TGG 0.570954 4_4:-682122 None:intergenic
AGATTATGTTGAGTACTACA+AGG 0.572264 4_4:-678383 None:intergenic
AAACAGACCCTAAAAGCCTA+AGG 0.572614 4_4:+678153 Msa0662830:five_prime_UTR
ATTGATTGAAACAAAAGCAA+AGG 0.575368 4_4:-682329 None:intergenic
CAATGGAATGCCTTTGTGGT+TGG 0.576689 4_4:-681256 None:intergenic
GAGACGGAGTAGTGATGGAT+TGG 0.577607 4_4:-681322 None:intergenic
TGGTAATTAGACCTTAAGCT+TGG 0.583935 4_4:-681431 None:intergenic
CCTGATCCTTATAATGTAGG+AGG 0.585582 4_4:+681674 Msa0662830:CDS
TGTGGCTATGGCTACAGCTG+AGG 0.587150 4_4:+679256 Msa0662830:CDS
AAGCTGCTGTGGAAGTTGCA+AGG 0.590980 4_4:+679297 Msa0662830:CDS
CAACCGGTGACCTTAGACGG+TGG 0.593652 4_4:+681843 Msa0662830:CDS
TAAGAGTTCAAATCATGCTA+GGG 0.594458 4_4:+679325 Msa0662830:CDS
AAGTTGCAAGCTTTAGTGAG+AGG 0.597297 4_4:+679826 Msa0662830:CDS
TTGTACCAAAATAGTAGGGG+TGG 0.602042 4_4:+681467 Msa0662830:CDS
AAGCAAGAGTTCTTGACCAA+AGG 0.602268 4_4:+679911 Msa0662830:CDS
TTGGTGTTTGAGAGGTGTGG+TGG 0.605794 4_4:-681412 None:intergenic
CAACCACCGTCTAAGGTCAC+CGG 0.606102 4_4:-681846 None:intergenic
GTTTGTGAGTGAGTGAGAGG+AGG 0.612712 4_4:-678298 None:intergenic
GTTCTGGCCTACCTCACTTG+GGG 0.613716 4_4:+682186 Msa0662830:three_prime_UTR
GCAAGCAACCGAGTCTGCAA+AGG 0.614885 4_4:+681556 Msa0662830:CDS
AAAGGTTCAACTTGGTTGGG+TGG 0.616143 4_4:-678718 None:intergenic
TTGTTATGACAGTCAAGAGA+AGG 0.620921 4_4:+680237 Msa0662830:intron
CATTATAAGGATCAGGAGCA+GGG 0.621699 4_4:-681667 None:intergenic
ATGGATGGCTACAAAACCAT+GGG 0.623432 4_4:+681100 Msa0662830:CDS
AGCAAGCAAAGATGACACTT+AGG 0.631862 4_4:+679863 Msa0662830:CDS
GATGGATGGCTACAAAACCA+TGG 0.632158 4_4:+681099 Msa0662830:CDS
ACAGCAGTGGGAGTGTCAGA+AGG 0.634678 4_4:-678180 None:intergenic
AAAATGGCTAGATAGATGGA+TGG 0.637956 4_4:+681085 Msa0662830:CDS
TTCGGTCGCAGAGTGCGCCA+AGG 0.638278 4_4:+681585 Msa0662830:CDS
AAGAGACTCTATTAAGACTG+TGG 0.640275 4_4:+681151 Msa0662830:CDS
CAACGGCTCTGCAACGACTG+CGG 0.642226 4_4:+681898 Msa0662830:three_prime_UTR
AAGGCCATCGACACCAGAGA+GGG 0.647934 4_4:+681610 Msa0662830:CDS
TTCAACACCCCAAGTGAGGT+AGG 0.648136 4_4:-682193 None:intergenic
GCATGAAGGTGGACAGCAGT+GGG 0.649814 4_4:-678192 None:intergenic
CTAAGGTCACCGGTTGACGA+AGG 0.652413 4_4:-681836 None:intergenic
AGTGATGGATTGGTTGTGAA+CGG 0.660603 4_4:-681312 None:intergenic
ATTGTTCAACACCCCAAGTG+AGG 0.663712 4_4:-682197 None:intergenic
CTATCTTCTCGAACACAACG+AGG 0.664998 4_4:-681383 None:intergenic
TATCTTCTCGAACACAACGA+GGG 0.667052 4_4:-681382 None:intergenic
ACAAAACCATGGGAGAATAG+GGG 0.672684 4_4:+681110 Msa0662830:CDS
ATCCACATCACCAACCACAA+AGG 0.673182 4_4:+681246 Msa0662830:CDS
AACTTGTACCAAAATAGTAG+GGG 0.675624 4_4:+681464 Msa0662830:CDS
TGAGAAACATGCTCTTGCTG+TGG 0.692861 4_4:+679238 Msa0662830:CDS
TGTTGTACTGAGAGTCTCGG+TGG 0.693541 4_4:+681803 Msa0662830:CDS
GGCATGAAGGTGGACAGCAG+TGG 0.698214 4_4:-678193 None:intergenic
TGTTGTTAATGCTACAAGTG+TGG 0.698782 4_4:+679196 Msa0662830:CDS
ATAAGGTCTACTTCACACGA+AGG 0.710315 4_4:+679934 Msa0662830:CDS
AAAGGCCATCGACACCAGAG+AGG 0.713109 4_4:+681609 Msa0662830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATGATTCAATATAA+TGG - chr4_4:678342-678361 None:intergenic 15.0%
!!! AATATTTTATAGTAGTACAT+TGG + chr4_4:678425-678444 Msa0662830:five_prime_UTR 15.0%
!! ATTAGAATGTAAAAACAAAA+GGG - chr4_4:679018-679037 None:intergenic 15.0%
!! AATTAGAATGTAAAAACAAA+AGG - chr4_4:679019-679038 None:intergenic 15.0%
!!! AATTTTGATTATAGTTATGT+TGG - chr4_4:680073-680092 None:intergenic 15.0%
!!! ATAGAACTTTTTAGTTATTT+TGG + chr4_4:680175-680194 Msa0662830:intron 15.0%
!!! AGAATTGATTTTGAATTTTT+TGG + chr4_4:680609-680628 Msa0662830:intron 15.0%
!!! ATATTTTGGAATTTCAATTA+TGG + chr4_4:682274-682293 Msa0662830:three_prime_UTR 15.0%
!!! AAATGTTGTTAAGTTTTACA+TGG + chr4_4:678816-678835 Msa0662830:exon 20.0%
!!! AATGTTGTTAAGTTTTACAT+GGG + chr4_4:678817-678836 Msa0662830:exon 20.0%
!!! TGTTTTTACATTCTAATTCA+TGG + chr4_4:679021-679040 Msa0662830:intron 20.0%
!!! AAAGTACCTTTTTTTTTTCT+TGG + chr4_4:679749-679768 Msa0662830:intron 20.0%
!! TTAGAAGCAAATATTATGTT+TGG + chr4_4:680117-680136 Msa0662830:intron 20.0%
!!! TGTGGATTATATTGATAATT+TGG - chr4_4:681233-681252 None:intergenic 20.0%
!!! ACCATTCACTTATTTTATTT+AGG + chr4_4:681960-681979 Msa0662830:three_prime_UTR 20.0%
!!! CACAATAACATGTAATATTT+TGG + chr4_4:682260-682279 Msa0662830:three_prime_UTR 20.0%
!! ATTATTACATTGCAAACAAA+AGG - chr4_4:682303-682322 None:intergenic 20.0%
! TTCATTTATTTGCACAAATG+AGG + chr4_4:678571-678590 Msa0662830:five_prime_UTR 25.0%
!! TAAAGTCTTTAAAGAAGAGA+AGG - chr4_4:678698-678717 None:intergenic 25.0%
!! ATGTTGTTAAGTTTTACATG+GGG + chr4_4:678818-678837 Msa0662830:exon 25.0%
!!! CTTTTTTCACTATCTTTTGT+TGG - chr4_4:678895-678914 None:intergenic 25.0%
! AAAGATAGTGAAAAAAGAAG+TGG + chr4_4:678898-678917 Msa0662830:CDS 25.0%
!!! AAAAAAGAAGGTGGATTTTT+AGG + chr4_4:679108-679127 Msa0662830:CDS 25.0%
!!! AACTTCTTTTAGAGGTTATT+TGG + chr4_4:679373-679392 Msa0662830:CDS 25.0%
! CTTTATGTTTGACAATATCA+TGG + chr4_4:679500-679519 Msa0662830:intron 25.0%
! AACACACCAAGAAAAAAAAA+AGG - chr4_4:679758-679777 None:intergenic 25.0%
!! AATATTTGCTTCTAAAAGCA+TGG - chr4_4:680112-680131 None:intergenic 25.0%
!!! TTGGAAACAAAAGTACTTTT+TGG + chr4_4:680136-680155 Msa0662830:intron 25.0%
!!! TATTTTGGTGTTCTGTTTAT+AGG + chr4_4:680190-680209 Msa0662830:intron 25.0%
!! TTATGTGTTTGAATCTTTTG+TGG + chr4_4:680214-680233 Msa0662830:intron 25.0%
! AGAAAATAAAGCGAAAAAGA+GGG - chr4_4:680402-680421 None:intergenic 25.0%
! AAGAAAATAAAGCGAAAAAG+AGG - chr4_4:680403-680422 None:intergenic 25.0%
!! ATTTTTCCTCATAGTGATTT+TGG - chr4_4:680461-680480 None:intergenic 25.0%
!! AGGAAAAATCTACATTTTGA+AGG + chr4_4:680472-680491 Msa0662830:intron 25.0%
! TCTAACTTGAAACTAGAATA+TGG + chr4_4:680793-680812 Msa0662830:intron 25.0%
!!! ATATTCAACTCACTTTTACA+TGG + chr4_4:680854-680873 Msa0662830:intron 25.0%
! ATTAGCTAAGAAAATTGCTT+TGG - chr4_4:680986-681005 None:intergenic 25.0%
!! AAGCAATTTTCTTAGCTAAT+TGG + chr4_4:680986-681005 Msa0662830:intron 25.0%
! ATTTGACAATTTCACAGATT+TGG + chr4_4:681009-681028 Msa0662830:intron 25.0%
! GAAATTATGGACATAGTTTA+AGG + chr4_4:681708-681727 Msa0662830:CDS 25.0%
! GCCTAAATAAAATAAGTGAA+TGG - chr4_4:681964-681983 None:intergenic 25.0%
!! ATGCTAACTTCTTTTCTTTT+GGG - chr4_4:682032-682051 None:intergenic 25.0%
!! TATGCTAACTTCTTTTCTTT+TGG - chr4_4:682033-682052 None:intergenic 25.0%
! AAAAGAAAAGAAGTTAGCAT+AGG + chr4_4:682032-682051 Msa0662830:three_prime_UTR 25.0%
! AAAGAAAAGAAGTTAGCATA+GGG + chr4_4:682033-682052 Msa0662830:three_prime_UTR 25.0%
! TAAAACCAAAACACAATACA+TGG - chr4_4:682125-682144 None:intergenic 25.0%
!!! GGTTTTATCATTTGTTTGTT+AGG + chr4_4:682138-682157 Msa0662830:three_prime_UTR 25.0%
! ATTGATTGAAACAAAAGCAA+AGG - chr4_4:682332-682351 None:intergenic 25.0%
ATCTAATTGTGCAAAGCTTA+TGG - chr4_4:678227-678246 None:intergenic 30.0%
AGATTATGTTGAGTACTACA+AGG - chr4_4:678386-678405 None:intergenic 30.0%
TCACAAAGAAACAAACAGTA+AGG + chr4_4:678468-678487 Msa0662830:five_prime_UTR 30.0%
TACACATTCAACAATGTTAC+AGG - chr4_4:678605-678624 None:intergenic 30.0%
AAAGCAAAAAGAAACACTTG+TGG - chr4_4:678663-678682 None:intergenic 30.0%
! TCAACACTGCAAAGTTTTTA+AGG + chr4_4:678747-678766 Msa0662830:five_prime_UTR 30.0%
TCAAGAAGAAGATGAAGAAA+AGG + chr4_4:678942-678961 Msa0662830:CDS 30.0%
TGTAGAAGAGAGAAAAAAGA+AGG + chr4_4:679096-679115 Msa0662830:intron 30.0%
TAAGAGTTCAAATCATGCTA+GGG + chr4_4:679325-679344 Msa0662830:CDS 30.0%
ATCTTCAAGACATTTCAGAT+AGG + chr4_4:680007-680026 Msa0662830:CDS 30.0%
! TAGTAAGTCATATGTTGCTT+TGG + chr4_4:680037-680056 Msa0662830:intron 30.0%
!!! CTTTTTGGTTAGAAAAGCAA+AGG + chr4_4:680151-680170 Msa0662830:intron 30.0%
AAGTAGTATTGCAGATGATT+GGG + chr4_4:680260-680279 Msa0662830:CDS 30.0%
! TTGTTCTAAGTCTGCATATT+TGG + chr4_4:680421-680440 Msa0662830:intron 30.0%
!!! GAATTTTTTGGATCTTCTCA+AGG + chr4_4:680621-680640 Msa0662830:intron 30.0%
TTCAATGTAATCACTAGTGT+CGG - chr4_4:680683-680702 None:intergenic 30.0%
! TTTGCCTCAACAATGATTTT+TGG + chr4_4:680820-680839 Msa0662830:intron 30.0%
GATACCAAAAATCATTGTTG+AGG - chr4_4:680827-680846 None:intergenic 30.0%
!! AAGAAAATTGCTTTGGTGTT+TGG - chr4_4:680979-680998 None:intergenic 30.0%
ATTTCACAGATTTGGAGAAA+TGG + chr4_4:681017-681036 Msa0662830:intron 30.0%
TCTCAACCTTATTCATATCT+AGG + chr4_4:681185-681204 Msa0662830:CDS 30.0%
TTTGCTCCTAGATATGAATA+AGG - chr4_4:681194-681213 None:intergenic 30.0%
AGGTCTAATTACCATTACAA+TGG + chr4_4:681440-681459 Msa0662830:CDS 30.0%
GGTCTAATTACCATTACAAT+GGG + chr4_4:681441-681460 Msa0662830:CDS 30.0%
GAACTTGTACCAAAATAGTA+GGG + chr4_4:681463-681482 Msa0662830:CDS 30.0%
AACTTGTACCAAAATAGTAG+GGG + chr4_4:681464-681483 Msa0662830:CDS 30.0%
AGTACAACAAGAAGAGTAAT+TGG - chr4_4:681792-681811 None:intergenic 30.0%
! TGCTAACTTCTTTTCTTTTG+GGG - chr4_4:682031-682050 None:intergenic 30.0%
GAACCTAAAAATCTGATGTT+AGG + chr4_4:682057-682076 Msa0662830:three_prime_UTR 30.0%
! ACTCCTAACATCAGATTTTT+AGG - chr4_4:682063-682082 None:intergenic 30.0%
ACAACAAGTATGTCCTTAAA+TGG + chr4_4:682227-682246 Msa0662830:three_prime_UTR 30.0%
! AGGTATACCTTAGGCTTTTA+GGG - chr4_4:678163-678182 None:intergenic 35.0%
! AAGGTATACCTTAGGCTTTT+AGG - chr4_4:678164-678183 None:intergenic 35.0%
! TATGGATTTTTGTGGCATGA+AGG - chr4_4:678209-678228 None:intergenic 35.0%
! GCAAAGCTTATGGATTTTTG+TGG - chr4_4:678217-678236 None:intergenic 35.0%
ATATAATGGTAGTCAGAGAC+AGG - chr4_4:678328-678347 None:intergenic 35.0%
AAGAAACACTTGTGGATCTT+TGG - chr4_4:678655-678674 None:intergenic 35.0%
AGAAGAGAAGGAGAAAAACA+AGG - chr4_4:678686-678705 None:intergenic 35.0%
! AAGTTTTACATGGGGAAGAA+AGG + chr4_4:678826-678845 Msa0662830:exon 35.0%
AGAAGAGAGAAAAAAGAAGG+TGG + chr4_4:679099-679118 Msa0662830:intron 35.0%
TTCACTGTTTCATGATTCAC+TGG - chr4_4:679137-679156 None:intergenic 35.0%
ACATCATGCTTCAACTTTGT+TGG - chr4_4:679179-679198 None:intergenic 35.0%
TGTTGTTAATGCTACAAGTG+TGG + chr4_4:679196-679215 Msa0662830:CDS 35.0%
CTAAGAGTTCAAATCATGCT+AGG + chr4_4:679324-679343 Msa0662830:CDS 35.0%
! GTGGTTCAAACTTCTTTTAG+AGG + chr4_4:679365-679384 Msa0662830:CDS 35.0%
AACAGATCTAATTGCCTGTT+TGG + chr4_4:679407-679426 Msa0662830:intron 35.0%
AACTTGCAGTAATTCCAAAC+AGG - chr4_4:679424-679443 None:intergenic 35.0%
! GTGTAGTTTTTGCCAAATCA+CGG - chr4_4:679647-679666 None:intergenic 35.0%
TGTGATTTCGTCAAATTCAC+TGG + chr4_4:679697-679716 Msa0662830:intron 35.0%
GTGATTTCGTCAAATTCACT+GGG + chr4_4:679698-679717 Msa0662830:intron 35.0%
TTGTTATGACAGTCAAGAGA+AGG + chr4_4:680237-680256 Msa0662830:intron 35.0%
GAAGTAGTATTGCAGATGAT+TGG + chr4_4:680259-680278 Msa0662830:CDS 35.0%
TTAACTTCTTCAACTGTGTG+TGG - chr4_4:680294-680313 None:intergenic 35.0%
ACACACAGTTGAAGAAGTTA+AGG + chr4_4:680293-680312 Msa0662830:CDS 35.0%
TTCTTTCCTTTGTTGCAACA+TGG - chr4_4:680319-680338 None:intergenic 35.0%
AACCTGTTGTGAAAATGCTT+GGG - chr4_4:680370-680389 None:intergenic 35.0%
AAACCTGTTGTGAAAATGCT+TGG - chr4_4:680371-680390 None:intergenic 35.0%
AGTTTGCCAAAATCACTATG+AGG + chr4_4:680452-680471 Msa0662830:intron 35.0%
! TGTATGTGTGTTTCCACTTT+TGG + chr4_4:680560-680579 Msa0662830:intron 35.0%
TCAAGGAGAATTGAGTTTGA+AGG + chr4_4:680638-680657 Msa0662830:intron 35.0%
AAGTTAGAACCTATGAAGCA+CGG - chr4_4:680781-680800 None:intergenic 35.0%
ATGAATTGACTGTGTAACAC+TGG - chr4_4:680929-680948 None:intergenic 35.0%
AATGGTAGGACATCATCAAT+TGG + chr4_4:681035-681054 Msa0662830:CDS 35.0%
AACTTGAAGAGAGACCAAAA+TGG + chr4_4:681069-681088 Msa0662830:CDS 35.0%
! ATCCATCTATCTAGCCATTT+TGG - chr4_4:681086-681105 None:intergenic 35.0%
AAAATGGCTAGATAGATGGA+TGG + chr4_4:681085-681104 Msa0662830:CDS 35.0%
TACAAAACCATGGGAGAATA+GGG + chr4_4:681109-681128 Msa0662830:CDS 35.0%
AAGAGACTCTATTAAGACTG+TGG + chr4_4:681151-681170 Msa0662830:CDS 35.0%
!! ATCCATTTTGGTGTGATCTA+TGG - chr4_4:681292-681311 None:intergenic 35.0%
! TGAATAGGTCTTGATTTTGC+TGG - chr4_4:681347-681366 None:intergenic 35.0%
AATCAAGACCTATTCAAGTC+CGG + chr4_4:681351-681370 Msa0662830:CDS 35.0%
TGGTAATTAGACCTTAAGCT+TGG - chr4_4:681434-681453 None:intergenic 35.0%
GGAACTTGTACCAAAATAGT+AGG + chr4_4:681462-681481 Msa0662830:CDS 35.0%
! AAGCCTTTTCTTTACTGATC+CGG - chr4_4:681642-681661 None:intergenic 35.0%
! GGAGTTGGTTATGGAAATTA+TGG + chr4_4:681695-681714 Msa0662830:CDS 35.0%
ACATTCACACTCTTGAAACT+TGG - chr4_4:681737-681756 None:intergenic 35.0%
AATGTAGCAGCATCACATTA+TGG + chr4_4:681752-681771 Msa0662830:CDS 35.0%
TCAACTACACTCAGTTTCAA+CGG + chr4_4:681881-681900 Msa0662830:three_prime_UTR 35.0%
GGTTACAACTATGACACATT+AGG + chr4_4:681928-681947 Msa0662830:three_prime_UTR 35.0%
!!! CTTTTCTTTTGGGGTGAAAA+GGG - chr4_4:682022-682041 None:intergenic 35.0%
!!! TCTTTTCTTTTGGGGTGAAA+AGG - chr4_4:682023-682042 None:intergenic 35.0%
! GAGAACCATGTATTGTGTTT+TGG + chr4_4:682117-682136 Msa0662830:three_prime_UTR 35.0%
!! TAAGGACATACTTGTTGTGT+AGG - chr4_4:682225-682244 None:intergenic 35.0%
!!! TTAGGCTTTTAGGGTCTGTT+TGG - chr4_4:678154-678173 None:intergenic 40.0%
AAACAGACCCTAAAAGCCTA+AGG + chr4_4:678153-678172 Msa0662830:five_prime_UTR 40.0%
! AGTGTCAGAAGGTATACCTT+AGG - chr4_4:678172-678191 None:intergenic 40.0%
CAGAAAGGTTCAACTTGGTT+GGG - chr4_4:678724-678743 None:intergenic 40.0%
ACAGAAAGGTTCAACTTGGT+TGG - chr4_4:678725-678744 None:intergenic 40.0%
GAAGACAGAAAGGTTCAACT+TGG - chr4_4:678729-678748 None:intergenic 40.0%
! TTGCAGTGTTGAAGACAGAA+AGG - chr4_4:678739-678758 None:intergenic 40.0%
GGAAGAAAGGTAGTAGTTCA+TGG + chr4_4:678839-678858 Msa0662830:CDS 40.0%
TTACAGTGACACCGTGATTT+CGG - chr4_4:679449-679468 None:intergenic 40.0%
TCCAAAATCATTGTGGCCAT+TGG - chr4_4:679590-679609 None:intergenic 40.0%
! TGGCCACAATGATTTTGGAT+CGG + chr4_4:679591-679610 Msa0662830:intron 40.0%
! TTTTGGATCGGTGGTGAATT+TGG + chr4_4:679603-679622 Msa0662830:intron 40.0%
CAAATCACGGTTGAACATTG+TGG - chr4_4:679634-679653 None:intergenic 40.0%
CACAATGTTCAACCGTGATT+TGG + chr4_4:679632-679651 Msa0662830:intron 40.0%
TTGACGAAATCACAGTGACA+CGG - chr4_4:679691-679710 None:intergenic 40.0%
TTGGTGTGTTTGAACCTTGA+AGG + chr4_4:679768-679787 Msa0662830:intron 40.0%
! AAGTTGCAAGCTTTAGTGAG+AGG + chr4_4:679826-679845 Msa0662830:CDS 40.0%
TAGTGAGAGGTCACAATGTT+AGG + chr4_4:679839-679858 Msa0662830:CDS 40.0%
AGCAAGCAAAGATGACACTT+AGG + chr4_4:679863-679882 Msa0662830:CDS 40.0%
AAGCAAGAGTTCTTGACCAA+AGG + chr4_4:679911-679930 Msa0662830:CDS 40.0%
GAGTTCTTGACCAAAGGATA+AGG + chr4_4:679917-679936 Msa0662830:CDS 40.0%
GTGAAGTAGACCTTATCCTT+TGG - chr4_4:679930-679949 None:intergenic 40.0%
ATAAGGTCTACTTCACACGA+AGG + chr4_4:679934-679953 Msa0662830:CDS 40.0%
! AACACTAGCAGTGTCACTAA+AGG - chr4_4:679974-679993 None:intergenic 40.0%
TTAGTGACACTGCTAGTGTT+TGG + chr4_4:679974-679993 Msa0662830:CDS 40.0%
TAGTGACACTGCTAGTGTTT+GGG + chr4_4:679975-679994 Msa0662830:CDS 40.0%
TTAAGGCCATGTTGCAACAA+AGG + chr4_4:680310-680329 Msa0662830:CDS 40.0%
GAAAGAAGCAGCAATGAAGA+GGG + chr4_4:680332-680351 Msa0662830:CDS 40.0%
!! ATGTGTGTTTCCACTTTTGG+AGG + chr4_4:680563-680582 Msa0662830:intron 40.0%
CAATTTCACTCCTCCAAAAG+TGG - chr4_4:680576-680595 None:intergenic 40.0%
CACAGATTTGGAGAAATGGT+AGG + chr4_4:681021-681040 Msa0662830:intron 40.0%
GACCAAAATGGCTAGATAGA+TGG + chr4_4:681081-681100 Msa0662830:CDS 40.0%
ATGGATGGCTACAAAACCAT+GGG + chr4_4:681100-681119 Msa0662830:CDS 40.0%
CTACAAAACCATGGGAGAAT+AGG + chr4_4:681108-681127 Msa0662830:CDS 40.0%
ACAAAACCATGGGAGAATAG+GGG + chr4_4:681110-681129 Msa0662830:CDS 40.0%
!! TTTTGGTGTGATCTATGGAG+CGG - chr4_4:681287-681306 None:intergenic 40.0%
CTCCATAGATCACACCAAAA+TGG + chr4_4:681287-681306 Msa0662830:CDS 40.0%
!! AGTGATGGATTGGTTGTGAA+CGG - chr4_4:681315-681334 None:intergenic 40.0%
TATCTTCTCGAACACAACGA+GGG - chr4_4:681385-681404 None:intergenic 40.0%
GGTACAAGTTCCCATTGTAA+TGG - chr4_4:681454-681473 None:intergenic 40.0%
TTGTACCAAAATAGTAGGGG+TGG + chr4_4:681467-681486 Msa0662830:CDS 40.0%
! TTGTTCCACCCCTACTATTT+TGG - chr4_4:681475-681494 None:intergenic 40.0%
TCGGTTGCTTGCATGTAATT+CGG - chr4_4:681548-681567 None:intergenic 40.0%
GATCCGGATCAGTAAAGAAA+AGG + chr4_4:681636-681655 Msa0662830:CDS 40.0%
CATTATAAGGATCAGGAGCA+GGG - chr4_4:681670-681689 None:intergenic 40.0%
ACATTATAAGGATCAGGAGC+AGG - chr4_4:681671-681690 None:intergenic 40.0%
GCTCCTGATCCTTATAATGT+AGG + chr4_4:681671-681690 Msa0662830:CDS 40.0%
CCTCCTACATTATAAGGATC+AGG - chr4_4:681677-681696 None:intergenic 40.0%
CCTGATCCTTATAATGTAGG+AGG + chr4_4:681674-681693 Msa0662830:CDS 40.0%
CCAACTCCTCCTACATTATA+AGG - chr4_4:681683-681702 None:intergenic 40.0%
CCTTATAATGTAGGAGGAGT+TGG + chr4_4:681680-681699 Msa0662830:CDS 40.0%
! AATGTAGGAGGAGTTGGTTA+TGG + chr4_4:681686-681705 Msa0662830:CDS 40.0%
AGCAGCATCACATTATGGAT+TGG + chr4_4:681757-681776 Msa0662830:CDS 40.0%
! TCTTGTTGTACTGAGAGTCT+CGG + chr4_4:681800-681819 Msa0662830:CDS 40.0%
GCAAGTACTATGTTGTTGCT+AGG + chr4_4:682089-682108 Msa0662830:three_prime_UTR 40.0%
TAGGATGCCAAGATAAATGC+TGG + chr4_4:682157-682176 Msa0662830:three_prime_UTR 40.0%
CATATGGCCAGCATTTATCT+TGG - chr4_4:682167-682186 None:intergenic 40.0%
TAAATGCTGGCCATATGTTC+TGG + chr4_4:682170-682189 Msa0662830:three_prime_UTR 40.0%
GTGACAAGACAGTCCATTTA+AGG - chr4_4:682243-682262 None:intergenic 40.0%
! GGATTTTTGTGGCATGAAGG+TGG - chr4_4:678206-678225 None:intergenic 45.0%
TGTGTTTGTGAGTGAGTGAG+AGG - chr4_4:678304-678323 None:intergenic 45.0%
AAAGGTTCAACTTGGTTGGG+TGG - chr4_4:678721-678740 None:intergenic 45.0%
ATGCTACAAGTGTGGCTTCA+AGG + chr4_4:679204-679223 Msa0662830:CDS 45.0%
TGAGAAACATGCTCTTGCTG+TGG + chr4_4:679238-679257 Msa0662830:CDS 45.0%
CTGCAAGTTTGCCGAAATCA+CGG + chr4_4:679435-679454 Msa0662830:intron 45.0%
ACTCATTGTGATCCTGCCAA+TGG + chr4_4:679571-679590 Msa0662830:intron 45.0%
AAATCATTGTGGCCATTGGC+AGG - chr4_4:679586-679605 None:intergenic 45.0%
! GCCAATGGCCACAATGATTT+TGG + chr4_4:679586-679605 Msa0662830:intron 45.0%
CCACCGATCCAAAATCATTG+TGG - chr4_4:679597-679616 None:intergenic 45.0%
! CCACAATGATTTTGGATCGG+TGG + chr4_4:679594-679613 Msa0662830:intron 45.0%
AAGTGCTCTTCTTGCCTTCA+AGG - chr4_4:679785-679804 None:intergenic 45.0%
GGAAAGAAGCAGCAATGAAG+AGG + chr4_4:680331-680350 Msa0662830:CDS 45.0%
! GTCCCAAGCATTTTCACAAC+AGG + chr4_4:680365-680384 Msa0662830:CDS 45.0%
CGATGAGTTCGACAAAGTCT+AGG - chr4_4:680516-680535 None:intergenic 45.0%
!! GTAATCACTAGTGTCGGTGT+CGG - chr4_4:680677-680696 None:intergenic 45.0%
TATCTGTGTCCGTGCTTCAT+AGG + chr4_4:680769-680788 Msa0662830:intron 45.0%
GATGGATGGCTACAAAACCA+TGG + chr4_4:681099-681118 Msa0662830:CDS 45.0%
ATCCACATCACCAACCACAA+AGG + chr4_4:681246-681265 Msa0662830:CDS 45.0%
CAATGGAATGCCTTTGTGGT+TGG - chr4_4:681259-681278 None:intergenic 45.0%
GAAGCAATGGAATGCCTTTG+TGG - chr4_4:681263-681282 None:intergenic 45.0%
! GGTTGTGAACGGATCCATTT+TGG - chr4_4:681304-681323 None:intergenic 45.0%
! GGTCTTGATTTTGCTGGAGA+CGG - chr4_4:681341-681360 None:intergenic 45.0%
CTATCTTCTCGAACACAACG+AGG - chr4_4:681386-681405 None:intergenic 45.0%
!! CCTTAAGCTTGGTGTTTGAG+AGG - chr4_4:681423-681442 None:intergenic 45.0%
CCTCTCAAACACCAAGCTTA+AGG + chr4_4:681420-681439 Msa0662830:CDS 45.0%
AGTAGGGGTGGAACAAGTAA+TGG + chr4_4:681479-681498 Msa0662830:CDS 45.0%
AATTCGAGCCTTTGCAGACT+CGG - chr4_4:681567-681586 None:intergenic 45.0%
AGTCTGCAAAGGCTCGAATT+CGG + chr4_4:681567-681586 Msa0662830:CDS 45.0%
ATTGTTCAACACCCCAAGTG+AGG - chr4_4:682200-682219 None:intergenic 45.0%
!!! TTTACATAATAATAAAATTT+TGG + chr4_4:678250-678269 Msa0662830:five_prime_UTR 5.0%
!!! ATTATATATTCATTTTTTTT+TGG + chr4_4:678501-678520 Msa0662830:five_prime_UTR 5.0%
GTTTGTGAGTGAGTGAGAGG+AGG - chr4_4:678301-678320 None:intergenic 50.0%
TGTGGCTTCAAGGACAGATC+AGG + chr4_4:679214-679233 Msa0662830:CDS 50.0%
! ACATGCTCTTGCTGTGGCTA+TGG + chr4_4:679244-679263 Msa0662830:CDS 50.0%
AAGCTGCTGTGGAAGTTGCA+AGG + chr4_4:679297-679316 Msa0662830:CDS 50.0%
! GGAGCATTATGCTGCTGTTG+TGG + chr4_4:679346-679365 Msa0662830:CDS 50.0%
!! AAGAGCACTTCGTGCGCTTA+AGG + chr4_4:679795-679814 Msa0662830:CDS 50.0%
! AGAGCACTTCGTGCGCTTAA+GGG + chr4_4:679796-679815 Msa0662830:CDS 50.0%
! CTGCTAGTGTTTGGGATTCG+CGG + chr4_4:679983-680002 Msa0662830:CDS 50.0%
! TTGAGTTTGAAGGCGTGTCC+CGG + chr4_4:680648-680667 Msa0662830:intron 50.0%
CTGACACAAACACGTCGACA+CGG - chr4_4:680731-680750 None:intergenic 50.0%
GTTTGTGTCAGTGTCGTGTC+CGG + chr4_4:680740-680759 Msa0662830:intron 50.0%
ACGGACACAGATACGTACAC+CGG - chr4_4:680762-680781 None:intergenic 50.0%
!! TGCCTTTGTGGTTGGTGATG+TGG - chr4_4:681251-681270 None:intergenic 50.0%
TCTATGGAGCGGTGAAGCAA+TGG - chr4_4:681276-681295 None:intergenic 50.0%
!! GAGACGGAGTAGTGATGGAT+TGG - chr4_4:681325-681344 None:intergenic 50.0%
CTTGACGACCGGACTTGAAT+AGG - chr4_4:681362-681381 None:intergenic 50.0%
! TTGGTGTTTGAGAGGTGTGG+TGG - chr4_4:681415-681434 None:intergenic 50.0%
!! AGCTTGGTGTTTGAGAGGTG+TGG - chr4_4:681418-681437 None:intergenic 50.0%
ACACCAGAGAGGGATAGATC+CGG + chr4_4:681620-681639 Msa0662830:CDS 50.0%
TGTTGTACTGAGAGTCTCGG+TGG + chr4_4:681803-681822 Msa0662830:CDS 50.0%
GAGGTTTCACCTTCGTCAAC+CGG + chr4_4:681827-681846 Msa0662830:CDS 50.0%
CTCATCTCAACCACCGTCTA+AGG - chr4_4:681856-681875 None:intergenic 50.0%
! TGCAACGACTGCGGAGTATT+CGG + chr4_4:681907-681926 Msa0662830:three_prime_UTR 50.0%
GTGAGGTAGGCCAGAACATA+TGG - chr4_4:682183-682202 None:intergenic 50.0%
ATGTTCTGGCCTACCTCACT+TGG + chr4_4:682184-682203 Msa0662830:three_prime_UTR 50.0%
TGTTCTGGCCTACCTCACTT+GGG + chr4_4:682185-682204 Msa0662830:three_prime_UTR 50.0%
TTCAACACCCCAAGTGAGGT+AGG - chr4_4:682196-682215 None:intergenic 50.0%
!! ACAGCAGTGGGAGTGTCAGA+AGG - chr4_4:678183-678202 None:intergenic 55.0%
GCATGAAGGTGGACAGCAGT+GGG - chr4_4:678195-678214 None:intergenic 55.0%
TGAGTGAGTGAGAGGAGGAG+GGG - chr4_4:678296-678315 None:intergenic 55.0%
GTGAGTGAGTGAGAGGAGGA+GGG - chr4_4:678297-678316 None:intergenic 55.0%
TGTGAGTGAGTGAGAGGAGG+AGG - chr4_4:678298-678317 None:intergenic 55.0%
TGTGGCTATGGCTACAGCTG+AGG + chr4_4:679256-679275 Msa0662830:CDS 55.0%
AGCTACTGCTCAAGCTGCTG+TGG + chr4_4:679286-679305 Msa0662830:CDS 55.0%
GAGCACTTCGTGCGCTTAAG+GGG + chr4_4:679797-679816 Msa0662830:CDS 55.0%
GCTCTTCCCCTATTCTCCCA+TGG - chr4_4:681119-681138 None:intergenic 55.0%
TGCTGGAGACGGAGTAGTGA+TGG - chr4_4:681330-681349 None:intergenic 55.0%
GCAAGCAACCGAGTCTGCAA+AGG + chr4_4:681556-681575 Msa0662830:CDS 55.0%
AAAGGCCATCGACACCAGAG+AGG + chr4_4:681609-681628 Msa0662830:CDS 55.0%
AAGGCCATCGACACCAGAGA+GGG + chr4_4:681610-681629 Msa0662830:CDS 55.0%
! CTATCCCTCTCTGGTGTCGA+TGG - chr4_4:681617-681636 None:intergenic 55.0%
GATCCGGATCTATCCCTCTC+TGG - chr4_4:681626-681645 None:intergenic 55.0%
! CTAAGGTCACCGGTTGACGA+AGG - chr4_4:681839-681858 None:intergenic 55.0%
CGTCAACCGGTGACCTTAGA+CGG + chr4_4:681840-681859 Msa0662830:CDS 55.0%
! CAACCACCGTCTAAGGTCAC+CGG - chr4_4:681849-681868 None:intergenic 55.0%
GTTCTGGCCTACCTCACTTG+GGG + chr4_4:682186-682205 Msa0662830:three_prime_UTR 55.0%
GGCATGAAGGTGGACAGCAG+TGG - chr4_4:678196-678215 None:intergenic 60.0%
GAGTGAGTGAGAGGAGGAGG+GGG - chr4_4:678295-678314 None:intergenic 60.0%
!! TAGTGTCGGTGTCGGACACC+GGG - chr4_4:680669-680688 None:intergenic 60.0%
!! CTAGTGTCGGTGTCGGACAC+CGG - chr4_4:680670-680689 None:intergenic 60.0%
CACAACGAGGGCTTGACGAC+CGG - chr4_4:681373-681392 None:intergenic 60.0%
!! GGTGTCGATGGCCTTTGCCT+TGG - chr4_4:681605-681624 None:intergenic 60.0%
TACTGAGAGTCTCGGTGGCG+AGG + chr4_4:681808-681827 Msa0662830:CDS 60.0%
CAACCGGTGACCTTAGACGG+TGG + chr4_4:681843-681862 Msa0662830:CDS 60.0%
CAACGGCTCTGCAACGACTG+CGG + chr4_4:681898-681917 Msa0662830:three_prime_UTR 60.0%
TTCGGTCGCAGAGTGCGCCA+AGG + chr4_4:681585-681604 Msa0662830:CDS 65.0%
CGCAGAGTGCGCCAAGGCAA+AGG + chr4_4:681591-681610 Msa0662830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 678133 682365 678133 ID=Msa0662830;Name=Msa0662830
chr4_4 mRNA 678133 682365 678133 ID=Msa0662830-mRNA-1;Parent=Msa0662830;Name=Msa0662830-mRNA-1;_AED=0.05;_eAED=0.05;_QI=702|1|1|1|1|1|5|491|552
chr4_4 exon 678133 678963 678133 ID=Msa0662830-mRNA-1:exon:282;Parent=Msa0662830-mRNA-1
chr4_4 exon 679101 679394 679101 ID=Msa0662830-mRNA-1:exon:283;Parent=Msa0662830-mRNA-1
chr4_4 exon 679790 680038 679790 ID=Msa0662830-mRNA-1:exon:284;Parent=Msa0662830-mRNA-1
chr4_4 exon 680249 680386 680249 ID=Msa0662830-mRNA-1:exon:285;Parent=Msa0662830-mRNA-1
chr4_4 exon 681026 682365 681026 ID=Msa0662830-mRNA-1:exon:286;Parent=Msa0662830-mRNA-1
chr4_4 five_prime_UTR 678133 678834 678133 ID=Msa0662830-mRNA-1:five_prime_utr;Parent=Msa0662830-mRNA-1
chr4_4 CDS 678835 678963 678835 ID=Msa0662830-mRNA-1:cds;Parent=Msa0662830-mRNA-1
chr4_4 CDS 679101 679394 679101 ID=Msa0662830-mRNA-1:cds;Parent=Msa0662830-mRNA-1
chr4_4 CDS 679790 680038 679790 ID=Msa0662830-mRNA-1:cds;Parent=Msa0662830-mRNA-1
chr4_4 CDS 680249 680386 680249 ID=Msa0662830-mRNA-1:cds;Parent=Msa0662830-mRNA-1
chr4_4 CDS 681026 681874 681026 ID=Msa0662830-mRNA-1:cds;Parent=Msa0662830-mRNA-1
chr4_4 three_prime_UTR 681875 682365 681875 ID=Msa0662830-mRNA-1:three_prime_utr;Parent=Msa0662830-mRNA-1
Gene Sequence

>Msa0662830

ATGGGGAAGAAAGGTAGTAGTTCATGGTTAACTGCTGTGAAAAGAGCATTCAGATCTCCAACAAAAGATAGTGAAAAAAGAAGTGGTAGAAGAAGAGAAGATTGTGATCAAGAAGAAGATGAAGAAAAGAAGAGAGAAAAAAGAAGGTGGATTTTTAGGAAACCAGTGAATCATGAAACAGTGAATAATACACAACAAAGTACACCAACAAAGTTGAAGCATGATGTTGTTAATGCTACAAGTGTGGCTTCAAGGACAGATCAGGATGAGAAACATGCTCTTGCTGTGGCTATGGCTACAGCTGAGGCAGCAAGAGCTACTGCTCAAGCTGCTGTGGAAGTTGCAAGGTTGACTAAGAGTTCAAATCATGCTAGGGAGCATTATGCTGCTGTTGTGGTTCAAACTTCTTTTAGAGGTTATTTGGCAAGAAGAGCACTTCGTGCGCTTAAGGGGTTAGTGAAGTTGCAAGCTTTAGTGAGAGGTCACAATGTTAGGAAGCAAGCAAAGATGACACTTAGGTGCATGCAAGCTCTTGTTAGAGTACAAGCAAGAGTTCTTGACCAAAGGATAAGGTCTACTTCACACGAAGGAAGTAGAAAATCAACCTTTAGTGACACTGCTAGTGTTTGGGATTCGCGGTATCTTCAAGACATTTCAGATAGGAAATCACTATCAAGAGAAGGAAGTAGTATTGCAGATGATTGGGATGATCGTCCACACACAGTTGAAGAAGTTAAGGCCATGTTGCAACAAAGGAAAGAAGCAGCAATGAAGAGGGAAAAGTCATTGTCCCAAGCATTTTCACAACAGATTTGGAGAAATGGTAGGACATCATCAATTGGAAATGAAGATGAACTTGAAGAGAGACCAAAATGGCTAGATAGATGGATGGCTACAAAACCATGGGAGAATAGGGGAAGAGCTTCAACTGATCAAAGAGACTCTATTAAGACTGTGGAAGTCGACACATCTCAACCTTATTCATATCTAGGAGCAAATTACAGAAGATCACATCCAAATTATCAATATAATCCACATCACCAACCACAAAGGCATTCCATTGCTTCACCGCTCCATAGATCACACCAAAATGGATCCGTTCACAACCAATCCATCACTACTCCGTCTCCAGCAAAATCAAGACCTATTCAAGTCCGGTCGTCAAGCCCTCGTTGTGTTCGAGAAGATAGAAGCTACCACCACACCTCTCAAACACCAAGCTTAAGGTCTAATTACCATTACAATGGGAACTTGTACCAAAATAGTAGGGGTGGAACAAGTAATGGTAGTAGTAACAGTATTGCTTCTACTACTGCTTCAGCTACATTGCCGAATTACATGCAAGCAACCGAGTCTGCAAAGGCTCGAATTCGGTCGCAGAGTGCGCCAAGGCAAAGGCCATCGACACCAGAGAGGGATAGATCCGGATCAGTAAAGAAAAGGCTTTCTTTCCCTGCTCCTGATCCTTATAATGTAGGAGGAGTTGGTTATGGAAATTATGGACATAGTTTAAGGAGTCCAAGTTTCAAGAGTGTGAATGTAGCAGCATCACATTATGGATTGGAGCAAAATTCCAATTACTCTTCTTGTTGTACTGAGAGTCTCGGTGGCGAGGTTTCACCTTCGTCAACCGGTGACCTTAGACGGTGGTTGAGATGA
Protein sequence

>Msa0662830

MGKKGSSSWLTAVKRAFRSPTKDSEKRSGRRREDCDQEEDEEKKREKRRWIFRKPVNHETVNNTQQSTPTKLKHDVVNATSVASRTDQDEKHALAVAMATAEAARATAQAAVEVARLTKSSNHAREHYAAVVVQTSFRGYLARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALVRVQARVLDQRIRSTSHEGSRKSTFSDTASVWDSRYLQDISDRKSLSREGSSIADDWDDRPHTVEEVKAMLQQRKEAAMKREKSLSQAFSQQIWRNGRTSSIGNEDELEERPKWLDRWMATKPWENRGRASTDQRDSIKTVEVDTSQPYSYLGANYRRSHPNYQYNPHHQPQRHSIASPLHRSHQNGSVHNQSITTPSPAKSRPIQVRSSSPRCVREDRSYHHTSQTPSLRSNYHYNGNLYQNSRGGTSNGSSNSIASTTASATLPNYMQATESAKARIRSQSAPRQRPSTPERDRSGSVKKRLSFPAPDPYNVGGVGYGNYGHSLRSPSFKSVNVAASHYGLEQNSNYSSCCTESLGGEVSPSSTGDLRRWLR*