AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1422730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422730 MtrunA17_Chr8g0346021 99.275 552 4 0 1 552 1 552 0.0 1135
Msa1422730 MtrunA17_Chr6g0462191 34.805 385 179 16 112 486 89 411 2.85e-36 140
Msa1422730 MtrunA17_Chr1g0181581 28.906 384 215 9 112 486 101 435 1.51e-34 135
Msa1422730 MtrunA17_Chr7g0263731 31.486 397 209 13 107 486 88 438 1.78e-34 135
Msa1422730 MtrunA17_Chr4g0025441 45.545 202 89 7 126 323 95 279 2.55e-34 134
Msa1422730 MtrunA17_Chr1g0200161 38.860 193 100 4 126 314 140 318 4.46e-33 132
Msa1422730 MtrunA17_Chr4g0045641 27.652 528 283 18 1 485 1 472 2.49e-32 130
Msa1422730 MtrunA17_Chr3g0126901 35.754 358 165 10 1 333 1 318 3.76e-32 128
Msa1422730 MtrunA17_Chr1g0146931 43.961 207 97 6 116 305 111 315 1.37e-28 119
Msa1422730 MtrunA17_Chr6g0472551 30.411 365 169 11 129 477 119 414 7.95e-27 113
Msa1422730 MtrunA17_Chr3g0135851 40.933 193 88 4 124 305 88 265 8.46e-27 113
Msa1422730 MtrunA17_Chr4g0020671 29.936 314 149 13 1 300 1 257 6.09e-25 107
Msa1422730 MtrunA17_Chr4g0057321 28.090 445 226 11 30 467 15 372 7.83e-25 107
Msa1422730 MtrunA17_Chr1g0204901 47.531 162 72 4 45 197 48 205 6.42e-24 104
Msa1422730 MtrunA17_Chr1g0203711 31.250 352 156 12 126 466 114 390 1.70e-22 100
Msa1422730 MtrunA17_Chr8g0340761 45.026 191 82 4 44 226 36 211 1.90e-22 99.8
Msa1422730 MtrunA17_Chr2g0283321 32.787 183 103 4 126 305 62 227 5.66e-22 96.7
Msa1422730 MtrunA17_Chr8g0342551 39.474 190 89 4 125 305 88 260 1.60e-20 94.4
Msa1422730 MtrunA17_Chr8g0389781 73.333 60 16 0 126 185 125 184 4.09e-20 93.2
Msa1422730 MtrunA17_Chr1g0166431 44.286 140 56 4 45 179 47 169 1.94e-19 91.3
Msa1422730 MtrunA17_Chr7g0273861 31.148 183 107 4 118 300 116 279 1.76e-18 88.2
Msa1422730 MtrunA17_Chr7g0273951 39.726 146 62 3 47 191 50 170 5.89e-18 85.9
Msa1422730 MtrunA17_Chr8g0371131 60.656 61 24 0 125 185 69 129 2.95e-17 79.0
Msa1422730 MtrunA17_Chr4g0060381 61.905 63 24 0 129 191 102 164 3.75e-17 84.7
Msa1422730 MtrunA17_Chr2g0330921 60.000 70 28 0 124 193 94 163 1.01e-16 82.0
Msa1422730 MtrunA17_Chr2g0330051 39.500 200 77 8 125 303 76 252 4.17e-16 80.5
Msa1422730 MtrunA17_Chr2g0316401 28.934 197 114 8 127 316 74 251 8.27e-16 78.6
Msa1422730 MtrunA17_Chr1g0156121 57.895 57 24 0 129 185 126 182 1.30e-13 73.9
Msa1422730 MtrunA17_Chr7g0215371 52.727 55 26 0 129 183 21 75 4.22e-11 59.7
Msa1422730 MtrunA17_Chr5g0416161 33.846 130 70 4 60 183 56 175 4.26e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422730 AT1G01110.2 57.913 575 171 23 1 552 1 527 0.0 526
Msa1422730 AT4G00820.1 58.007 562 180 18 9 552 11 534 1.62e-180 519
Msa1422730 AT1G01110.1 59.446 397 114 16 170 552 1 364 2.62e-137 402
Msa1422730 AT4G10640.1 36.219 566 204 19 1 552 1 423 2.85e-89 281
Msa1422730 AT3G49380.1 37.959 245 113 5 1 239 1 212 2.83e-37 141
Msa1422730 AT2G26180.1 43.627 204 89 4 119 311 76 264 2.90e-35 137
Msa1422730 AT3G49260.1 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1422730 AT3G49260.4 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1422730 AT3G49260.2 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1422730 AT5G03040.1 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1422730 AT5G03040.3 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1422730 AT5G03040.2 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1422730 AT3G49260.3 34.501 371 181 14 1 331 1 349 2.89e-34 135
Msa1422730 AT3G22190.3 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1422730 AT3G22190.2 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1422730 AT3G22190.4 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1422730 AT3G22190.1 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1422730 AT1G72670.1 37.500 208 104 6 107 305 73 263 1.95e-29 120
Msa1422730 AT3G52290.1 33.228 316 155 13 1 304 1 272 8.87e-29 119
Msa1422730 AT3G59690.2 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa1422730 AT3G59690.1 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa1422730 AT2G43680.4 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1422730 AT2G43680.2 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1422730 AT2G43680.3 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1422730 AT2G43680.5 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1422730 AT2G43680.1 26.619 417 225 14 126 539 322 660 2.18e-25 111
Msa1422730 AT5G13460.1 29.710 276 153 7 38 300 18 265 4.43e-23 102
Msa1422730 AT4G14750.1 68.182 66 21 0 126 191 104 169 4.45e-22 99.0
Msa1422730 AT1G17480.1 36.842 171 81 4 100 270 68 211 9.18e-22 97.8
Msa1422730 AT4G14750.3 68.182 66 21 0 126 191 163 228 1.04e-21 98.6
Msa1422730 AT4G14750.2 68.182 66 21 0 126 191 163 228 1.09e-21 98.6
Msa1422730 AT3G09710.2 30.732 205 118 7 111 303 95 287 1.35e-21 98.2
Msa1422730 AT3G09710.1 30.653 199 112 7 111 303 95 273 1.73e-21 97.8
Msa1422730 AT2G26410.2 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa1422730 AT2G26410.1 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa1422730 AT3G16490.1 65.714 70 24 0 121 190 101 170 1.79e-19 91.3
Msa1422730 AT4G23060.1 28.255 361 165 11 114 467 152 425 9.90e-19 89.7
Msa1422730 AT4G29150.1 44.262 122 57 2 122 241 126 238 2.19e-18 87.8
Msa1422730 AT5G07240.1 67.742 62 20 0 124 185 103 164 5.83e-18 86.7
Msa1422730 AT5G07240.2 67.742 62 20 0 124 185 149 210 7.49e-18 86.7
Msa1422730 AT5G62070.1 70.690 58 17 0 124 181 114 171 7.25e-17 83.2
Msa1422730 AT1G18840.1 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1422730 AT1G18840.3 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1422730 AT1G18840.2 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1422730 AT1G19870.1 35.556 135 70 2 71 205 173 290 7.50e-16 81.6
Msa1422730 AT2G33990.1 26.601 203 129 4 126 326 46 230 1.58e-15 77.4
Msa1422730 AT1G51960.1 60.000 65 26 0 126 190 99 163 1.93e-15 78.6
Msa1422730 AT1G19870.2 53.333 75 32 1 131 205 36 107 3.36e-15 79.0
Msa1422730 AT3G15050.1 26.630 184 115 4 129 308 52 219 1.33e-14 74.3
Msa1422730 AT1G74690.1 55.385 65 29 0 127 191 113 177 2.72e-14 76.3
Msa1422730 AT3G51380.1 48.235 85 43 1 108 192 19 102 3.14e-14 69.3
Msa1422730 AT1G14380.1 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1422730 AT1G14380.3 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1422730 AT1G14380.4 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1422730 AT1G14380.6 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1422730 AT1G14380.5 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1422730 AT1G14380.2 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1422730 AT1G14380.7 50.769 65 32 0 129 193 96 160 1.56e-13 73.9

Find 175 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATAACATGTAATATTT+TGG 0.059916 tig0025447:+20822 Msa1422730:three_prime_UTR
TTTACATAATAATAAAATTT+TGG 0.148736 tig0025447:+16812 Msa1422730:five_prime_UTR
GTGACAAGACAGTCCATTTA+AGG 0.176423 tig0025447:-20802 None:intergenic
GAAATTATGGACATAGTTTA+AGG 0.213922 tig0025447:+20270 Msa1422730:CDS
TTGTTCCACCCCTACTATTT+TGG 0.218545 tig0025447:-20034 None:intergenic
ATCCATCTATCTAGCCATTT+TGG 0.221443 tig0025447:-19645 None:intergenic
ATCTAATTGTGCAAAGCTTA+TGG 0.234585 tig0025447:-16786 None:intergenic
TGTGGATTATATTGATAATT+TGG 0.238180 tig0025447:-19792 None:intergenic
AAGAAACACTTGTGGATCTT+TGG 0.252655 tig0025447:-17214 None:intergenic
ACAACAAGTATGTCCTTAAA+TGG 0.267281 tig0025447:+20789 Msa1422730:three_prime_UTR
AGTACAACAAGAAGAGTAAT+TGG 0.281665 tig0025447:-20351 None:intergenic
CCAACTCCTCCTACATTATA+AGG 0.303150 tig0025447:-20242 None:intergenic
GGAGTTGGTTATGGAAATTA+TGG 0.312677 tig0025447:+20257 Msa1422730:CDS
TAGTGAGAGGTCACAATGTT+AGG 0.313052 tig0025447:+18401 Msa1422730:CDS
TAGTGACACTGCTAGTGTTT+GGG 0.318138 tig0025447:+18537 Msa1422730:CDS
AAGAGCACTTCGTGCGCTTA+AGG 0.319760 tig0025447:+18357 Msa1422730:CDS
TGATTTATGATTCAATATAA+TGG 0.328529 tig0025447:-16901 None:intergenic
CTCCATAGATCACACCAAAA+TGG 0.329388 tig0025447:+19849 Msa1422730:CDS
GGTTGTGAACGGATCCATTT+TGG 0.332251 tig0025447:-19863 None:intergenic
TACAAAACCATGGGAGAATA+GGG 0.336323 tig0025447:+19671 Msa1422730:CDS
GAGAACCATGTATTGTGTTT+TGG 0.353766 tig0025447:+20679 Msa1422730:three_prime_UTR
TGCAACGACTGCGGAGTATT+CGG 0.354857 tig0025447:+20469 Msa1422730:three_prime_UTR
GAAGTAGTATTGCAGATGAT+TGG 0.362742 tig0025447:+18821 Msa1422730:CDS
CTCATCTCAACCACCGTCTA+AGG 0.364361 tig0025447:-20415 None:intergenic
TACACATTCAACAATGTTAC+AGG 0.365782 tig0025447:-17164 None:intergenic
GGTTACAACTATGACACATT+AGG 0.366301 tig0025447:+20490 Msa1422730:three_prime_UTR
CTACAAAACCATGGGAGAAT+AGG 0.367491 tig0025447:+19670 Msa1422730:CDS
ACATCATGCTTCAACTTTGT+TGG 0.375678 tig0025447:-17738 None:intergenic
TTTGCTCCTAGATATGAATA+AGG 0.377417 tig0025447:-19753 None:intergenic
TCTCAACCTTATTCATATCT+AGG 0.385222 tig0025447:+19747 Msa1422730:CDS
GGAACTTGTACCAAAATAGT+AGG 0.389102 tig0025447:+20024 Msa1422730:CDS
TTAGTGACACTGCTAGTGTT+TGG 0.389305 tig0025447:+18536 Msa1422730:CDS
TCGGTTGCTTGCATGTAATT+CGG 0.404052 tig0025447:-20107 None:intergenic
TGTGAGTGAGTGAGAGGAGG+AGG 0.410940 tig0025447:-16857 None:intergenic
AATGTAGGAGGAGTTGGTTA+TGG 0.413219 tig0025447:+20248 Msa1422730:CDS
ATGCTACAAGTGTGGCTTCA+AGG 0.418682 tig0025447:+17766 Msa1422730:CDS
GAGGTTTCACCTTCGTCAAC+CGG 0.431189 tig0025447:+20389 Msa1422730:CDS
TTCTTTCCTTTGTTGCAACA+TGG 0.431704 tig0025447:-18878 None:intergenic
ATTATTACATTGCAAACAAA+AGG 0.433968 tig0025447:-20862 None:intergenic
CTTGACGACCGGACTTGAAT+AGG 0.434489 tig0025447:-19921 None:intergenic
ATGTTCTGGCCTACCTCACT+TGG 0.436846 tig0025447:+20746 Msa1422730:three_prime_UTR
CCTCTCAAACACCAAGCTTA+AGG 0.437895 tig0025447:+19982 Msa1422730:CDS
GGTGTCGATGGCCTTTGCCT+TGG 0.439851 tig0025447:-20164 None:intergenic
GTGAGGTAGGCCAGAACATA+TGG 0.440826 tig0025447:-20742 None:intergenic
GACCAAAATGGCTAGATAGA+TGG 0.442192 tig0025447:+19643 Msa1422730:CDS
TTCACTGTTTCATGATTCAC+TGG 0.442484 tig0025447:-17696 None:intergenic
CAGAAAGGTTCAACTTGGTT+GGG 0.443221 tig0025447:-17283 None:intergenic
CCTCCTACATTATAAGGATC+AGG 0.445401 tig0025447:-20236 None:intergenic
CATATGGCCAGCATTTATCT+TGG 0.445786 tig0025447:-20726 None:intergenic
AAGTAGTATTGCAGATGATT+GGG 0.451725 tig0025447:+18822 Msa1422730:CDS
GGTACAAGTTCCCATTGTAA+TGG 0.452470 tig0025447:-20013 None:intergenic
TGCCTTTGTGGTTGGTGATG+TGG 0.455595 tig0025447:-19810 None:intergenic
AAACCTGTTGTGAAAATGCT+TGG 0.456509 tig0025447:-18930 None:intergenic
CCTTATAATGTAGGAGGAGT+TGG 0.456681 tig0025447:+20242 Msa1422730:CDS
TTCATTTATTTGCACAAATG+AGG 0.457015 tig0025447:+17133 Msa1422730:five_prime_UTR
TAAATGCTGGCCATATGTTC+TGG 0.460943 tig0025447:+20732 Msa1422730:three_prime_UTR
GGTCTAATTACCATTACAAT+GGG 0.460991 tig0025447:+20003 Msa1422730:CDS
ACATTCACACTCTTGAAACT+TGG 0.462543 tig0025447:-20296 None:intergenic
CTGCTAGTGTTTGGGATTCG+CGG 0.462678 tig0025447:+18545 Msa1422730:CDS
AGAGCACTTCGTGCGCTTAA+GGG 0.465270 tig0025447:+18358 Msa1422730:CDS
GAGTGAGTGAGAGGAGGAGG+GGG 0.466047 tig0025447:-16854 None:intergenic
GGAAGAAAGGTAGTAGTTCA+TGG 0.469859 tig0025447:+17401 Msa1422730:CDS
AATTCGAGCCTTTGCAGACT+CGG 0.472168 tig0025447:-20126 None:intergenic
ACAGAAAGGTTCAACTTGGT+TGG 0.472834 tig0025447:-17284 None:intergenic
TCAAGAAGAAGATGAAGAAA+AGG 0.473802 tig0025447:+17504 Msa1422730:CDS
GTGAGTGAGTGAGAGGAGGA+GGG 0.475121 tig0025447:-16856 None:intergenic
ACACACAGTTGAAGAAGTTA+AGG 0.475450 tig0025447:+18855 Msa1422730:CDS
ATTTCACAGATTTGGAGAAA+TGG 0.475842 tig0025447:+19579 Msa1422730:intron
AATGGTAGGACATCATCAAT+TGG 0.477140 tig0025447:+19597 Msa1422730:CDS
GGAAAGAAGCAGCAATGAAG+AGG 0.480771 tig0025447:+18893 Msa1422730:CDS
CGCAGAGTGCGCCAAGGCAA+AGG 0.484032 tig0025447:+20153 Msa1422730:CDS
CACAACGAGGGCTTGACGAC+CGG 0.488125 tig0025447:-19932 None:intergenic
TGTGGCTTCAAGGACAGATC+AGG 0.489395 tig0025447:+17776 Msa1422730:CDS
ACATTATAAGGATCAGGAGC+AGG 0.491564 tig0025447:-20230 None:intergenic
AATGTAGCAGCATCACATTA+TGG 0.491586 tig0025447:+20314 Msa1422730:CDS
TGTTCTGGCCTACCTCACTT+GGG 0.492874 tig0025447:+20747 Msa1422730:three_prime_UTR
CGTCAACCGGTGACCTTAGA+CGG 0.494779 tig0025447:+20402 Msa1422730:CDS
CTATCCCTCTCTGGTGTCGA+TGG 0.494815 tig0025447:-20176 None:intergenic
TGAGTGAGTGAGAGGAGGAG+GGG 0.494935 tig0025447:-16855 None:intergenic
TAAAGTCTTTAAAGAAGAGA+AGG 0.495381 tig0025447:-17257 None:intergenic
GAACTTGTACCAAAATAGTA+GGG 0.496333 tig0025447:+20025 Msa1422730:CDS
GTGAAGTAGACCTTATCCTT+TGG 0.496611 tig0025447:-18489 None:intergenic
GATCCGGATCTATCCCTCTC+TGG 0.498338 tig0025447:-20185 None:intergenic
GATCCGGATCAGTAAAGAAA+AGG 0.499138 tig0025447:+20198 Msa1422730:CDS
GCTCTTCCCCTATTCTCCCA+TGG 0.500010 tig0025447:-19678 None:intergenic
ACATGCTCTTGCTGTGGCTA+TGG 0.501053 tig0025447:+17806 Msa1422730:CDS
CTAAGAGTTCAAATCATGCT+AGG 0.504978 tig0025447:+17886 Msa1422730:CDS
AGTCTGCAAAGGCTCGAATT+CGG 0.506521 tig0025447:+20129 Msa1422730:CDS
TAAGGACATACTTGTTGTGT+AGG 0.509479 tig0025447:-20784 None:intergenic
TTGCAGTGTTGAAGACAGAA+AGG 0.511229 tig0025447:-17298 None:intergenic
AACTTGAAGAGAGACCAAAA+TGG 0.518980 tig0025447:+19631 Msa1422730:CDS
TGCTGGAGACGGAGTAGTGA+TGG 0.522782 tig0025447:-19889 None:intergenic
GCTCCTGATCCTTATAATGT+AGG 0.524729 tig0025447:+20233 Msa1422730:CDS
CCTTAAGCTTGGTGTTTGAG+AGG 0.526162 tig0025447:-19982 None:intergenic
GAAGCAATGGAATGCCTTTG+TGG 0.526434 tig0025447:-19822 None:intergenic
AACCTGTTGTGAAAATGCTT+GGG 0.527631 tig0025447:-18929 None:intergenic
AGGTCTAATTACCATTACAA+TGG 0.529005 tig0025447:+20002 Msa1422730:CDS
GAGTTCTTGACCAAAGGATA+AGG 0.529710 tig0025447:+18479 Msa1422730:CDS
ATCTTCAAGACATTTCAGAT+AGG 0.531548 tig0025447:+18569 Msa1422730:CDS
AGCTACTGCTCAAGCTGCTG+TGG 0.535167 tig0025447:+17848 Msa1422730:CDS
GCCTAAATAAAATAAGTGAA+TGG 0.536968 tig0025447:-20523 None:intergenic
TCACAAAGAAACAAACAGTA+AGG 0.537387 tig0025447:+17030 Msa1422730:five_prime_UTR
ATATAATGGTAGTCAGAGAC+AGG 0.538519 tig0025447:-16887 None:intergenic
AAAGAAAAGAAGTTAGCATA+GGG 0.539161 tig0025447:+20595 Msa1422730:three_prime_UTR
AGTGTCAGAAGGTATACCTT+AGG 0.542037 tig0025447:-16731 None:intergenic
AAAAGAAAAGAAGTTAGCAT+AGG 0.544970 tig0025447:+20594 Msa1422730:three_prime_UTR
GAGCACTTCGTGCGCTTAAG+GGG 0.545080 tig0025447:+18359 Msa1422730:CDS
AGCAGCATCACATTATGGAT+TGG 0.546365 tig0025447:+20319 Msa1422730:CDS
TCTTGTTGTACTGAGAGTCT+CGG 0.552055 tig0025447:+20362 Msa1422730:CDS
GGAGCATTATGCTGCTGTTG+TGG 0.552104 tig0025447:+17908 Msa1422730:CDS
AGCTTGGTGTTTGAGAGGTG+TGG 0.552600 tig0025447:-19977 None:intergenic
TTAACTTCTTCAACTGTGTG+TGG 0.553478 tig0025447:-18853 None:intergenic
GCAAGTACTATGTTGTTGCT+AGG 0.553496 tig0025447:+20651 Msa1422730:three_prime_UTR
AACACTAGCAGTGTCACTAA+AGG 0.555204 tig0025447:-18533 None:intergenic
AATCAAGACCTATTCAAGTC+CGG 0.556812 tig0025447:+19913 Msa1422730:CDS
TACTGAGAGTCTCGGTGGCG+AGG 0.556933 tig0025447:+20370 Msa1422730:CDS
ACACCAGAGAGGGATAGATC+CGG 0.558129 tig0025447:+20182 Msa1422730:CDS
GAAAGAAGCAGCAATGAAGA+GGG 0.561558 tig0025447:+18894 Msa1422730:CDS
AGTAGGGGTGGAACAAGTAA+TGG 0.561747 tig0025447:+20041 Msa1422730:CDS
TTAAGGCCATGTTGCAACAA+AGG 0.561941 tig0025447:+18872 Msa1422730:CDS
GGATGATCAAAGAAAGTGAA+AGG 0.565239 tig0025447:-16692 None:intergenic
TGTGTTTGTGAGTGAGTGAG+AGG 0.565316 tig0025447:-16863 None:intergenic
GAAGACAGAAAGGTTCAACT+TGG 0.566144 tig0025447:-17288 None:intergenic
CACAGATTTGGAGAAATGGT+AGG 0.567014 tig0025447:+19583 Msa1422730:intron
TCAACTACACTCAGTTTCAA+CGG 0.568119 tig0025447:+20443 Msa1422730:three_prime_UTR
TAGGATGCCAAGATAAATGC+TGG 0.570025 tig0025447:+20719 Msa1422730:three_prime_UTR
TCTATGGAGCGGTGAAGCAA+TGG 0.570311 tig0025447:-19835 None:intergenic
TAAAACCAAAACACAATACA+TGG 0.570954 tig0025447:-20684 None:intergenic
AGATTATGTTGAGTACTACA+AGG 0.572264 tig0025447:-16945 None:intergenic
AAACAGACCCTAAAAGCCTA+AGG 0.572614 tig0025447:+16715 Msa1422730:five_prime_UTR
ATTGATTGAAACAAAAGCAA+AGG 0.575368 tig0025447:-20891 None:intergenic
CAATGGAATGCCTTTGTGGT+TGG 0.576689 tig0025447:-19818 None:intergenic
GAGACGGAGTAGTGATGGAT+TGG 0.577607 tig0025447:-19884 None:intergenic
TGGTAATTAGACCTTAAGCT+TGG 0.583935 tig0025447:-19993 None:intergenic
CCTGATCCTTATAATGTAGG+AGG 0.585582 tig0025447:+20236 Msa1422730:CDS
TGTGGCTATGGCTACAGCTG+AGG 0.587150 tig0025447:+17818 Msa1422730:CDS
AAGCTGCTGTGGAAGTTGCA+AGG 0.590980 tig0025447:+17859 Msa1422730:CDS
CAACCGGTGACCTTAGACGG+TGG 0.593652 tig0025447:+20405 Msa1422730:CDS
TAAGAGTTCAAATCATGCTA+GGG 0.594458 tig0025447:+17887 Msa1422730:CDS
AAGTTGCAAGCTTTAGTGAG+AGG 0.597297 tig0025447:+18388 Msa1422730:CDS
TTGTACCAAAATAGTAGGGG+TGG 0.602042 tig0025447:+20029 Msa1422730:CDS
AAGCAAGAGTTCTTGACCAA+AGG 0.602268 tig0025447:+18473 Msa1422730:CDS
TTGGTGTTTGAGAGGTGTGG+TGG 0.605794 tig0025447:-19974 None:intergenic
CAACCACCGTCTAAGGTCAC+CGG 0.606102 tig0025447:-20408 None:intergenic
GTTTGTGAGTGAGTGAGAGG+AGG 0.612712 tig0025447:-16860 None:intergenic
GTTCTGGCCTACCTCACTTG+GGG 0.613716 tig0025447:+20748 Msa1422730:three_prime_UTR
GCAAGCAACCGAGTCTGCAA+AGG 0.614885 tig0025447:+20118 Msa1422730:CDS
AAAGGTTCAACTTGGTTGGG+TGG 0.616143 tig0025447:-17280 None:intergenic
TTGTTATGACAGTCAAGAGA+AGG 0.620921 tig0025447:+18799 Msa1422730:intron
CATTATAAGGATCAGGAGCA+GGG 0.621699 tig0025447:-20229 None:intergenic
ATGGATGGCTACAAAACCAT+GGG 0.623432 tig0025447:+19662 Msa1422730:CDS
AGCAAGCAAAGATGACACTT+AGG 0.631862 tig0025447:+18425 Msa1422730:CDS
GATGGATGGCTACAAAACCA+TGG 0.632158 tig0025447:+19661 Msa1422730:CDS
ACAGCAGTGGGAGTGTCAGA+AGG 0.634678 tig0025447:-16742 None:intergenic
AAAATGGCTAGATAGATGGA+TGG 0.637956 tig0025447:+19647 Msa1422730:CDS
TTCGGTCGCAGAGTGCGCCA+AGG 0.638278 tig0025447:+20147 Msa1422730:CDS
AAGAGACTCTATTAAGACTG+TGG 0.640275 tig0025447:+19713 Msa1422730:CDS
CAACGGCTCTGCAACGACTG+CGG 0.642226 tig0025447:+20460 Msa1422730:three_prime_UTR
AAGGCCATCGACACCAGAGA+GGG 0.647934 tig0025447:+20172 Msa1422730:CDS
TTCAACACCCCAAGTGAGGT+AGG 0.648136 tig0025447:-20755 None:intergenic
GCATGAAGGTGGACAGCAGT+GGG 0.649814 tig0025447:-16754 None:intergenic
CTAAGGTCACCGGTTGACGA+AGG 0.652413 tig0025447:-20398 None:intergenic
AGTGATGGATTGGTTGTGAA+CGG 0.660603 tig0025447:-19874 None:intergenic
ATTGTTCAACACCCCAAGTG+AGG 0.663712 tig0025447:-20759 None:intergenic
CTATCTTCTCGAACACAACG+AGG 0.664998 tig0025447:-19945 None:intergenic
TATCTTCTCGAACACAACGA+GGG 0.667052 tig0025447:-19944 None:intergenic
ACAAAACCATGGGAGAATAG+GGG 0.672684 tig0025447:+19672 Msa1422730:CDS
ATCCACATCACCAACCACAA+AGG 0.673182 tig0025447:+19808 Msa1422730:CDS
AACTTGTACCAAAATAGTAG+GGG 0.675624 tig0025447:+20026 Msa1422730:CDS
TGAGAAACATGCTCTTGCTG+TGG 0.692861 tig0025447:+17800 Msa1422730:CDS
TGTTGTACTGAGAGTCTCGG+TGG 0.693541 tig0025447:+20365 Msa1422730:CDS
GGCATGAAGGTGGACAGCAG+TGG 0.698214 tig0025447:-16755 None:intergenic
TGTTGTTAATGCTACAAGTG+TGG 0.698782 tig0025447:+17758 Msa1422730:CDS
ATAAGGTCTACTTCACACGA+AGG 0.710315 tig0025447:+18496 Msa1422730:CDS
AAAGGCCATCGACACCAGAG+AGG 0.713109 tig0025447:+20171 Msa1422730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATGATTCAATATAA+TGG - tig0025447:16904-16923 None:intergenic 15.0%
!!! AATATTTTATAGTAGTACAT+TGG + tig0025447:16987-17006 Msa1422730:five_prime_UTR 15.0%
!! ATTAGAATGTAAAAACAAAA+GGG - tig0025447:17580-17599 None:intergenic 15.0%
!! AATTAGAATGTAAAAACAAA+AGG - tig0025447:17581-17600 None:intergenic 15.0%
!!! AATTTTGATTATAGTTATGT+TGG - tig0025447:18635-18654 None:intergenic 15.0%
!!! ATAGAACTTTTTAGTTATTT+TGG + tig0025447:18737-18756 Msa1422730:intron 15.0%
!!! AGAATTGATTTTGAATTTTT+TGG + tig0025447:19171-19190 Msa1422730:intron 15.0%
!!! ATATTTTGGAATTTCAATTA+TGG + tig0025447:20836-20855 Msa1422730:three_prime_UTR 15.0%
!!! AAATGTTGTTAAGTTTTACA+TGG + tig0025447:17378-17397 Msa1422730:exon 20.0%
!!! AATGTTGTTAAGTTTTACAT+GGG + tig0025447:17379-17398 Msa1422730:exon 20.0%
!!! TGTTTTTACATTCTAATTCA+TGG + tig0025447:17583-17602 Msa1422730:intron 20.0%
!!! AAAGTACCTTTTTTTTTTCT+TGG + tig0025447:18311-18330 Msa1422730:intron 20.0%
!! TTAGAAGCAAATATTATGTT+TGG + tig0025447:18679-18698 Msa1422730:intron 20.0%
!!! TGTGGATTATATTGATAATT+TGG - tig0025447:19795-19814 None:intergenic 20.0%
!!! ACCATTCACTTATTTTATTT+AGG + tig0025447:20522-20541 Msa1422730:three_prime_UTR 20.0%
!!! CACAATAACATGTAATATTT+TGG + tig0025447:20822-20841 Msa1422730:three_prime_UTR 20.0%
!! ATTATTACATTGCAAACAAA+AGG - tig0025447:20865-20884 None:intergenic 20.0%
! TTCATTTATTTGCACAAATG+AGG + tig0025447:17133-17152 Msa1422730:five_prime_UTR 25.0%
!! TAAAGTCTTTAAAGAAGAGA+AGG - tig0025447:17260-17279 None:intergenic 25.0%
!! ATGTTGTTAAGTTTTACATG+GGG + tig0025447:17380-17399 Msa1422730:exon 25.0%
!!! CTTTTTTCACTATCTTTTGT+TGG - tig0025447:17457-17476 None:intergenic 25.0%
! AAAGATAGTGAAAAAAGAAG+TGG + tig0025447:17460-17479 Msa1422730:CDS 25.0%
!!! AAAAAAGAAGGTGGATTTTT+AGG + tig0025447:17670-17689 Msa1422730:CDS 25.0%
!!! AACTTCTTTTAGAGGTTATT+TGG + tig0025447:17935-17954 Msa1422730:CDS 25.0%
! CTTTATGTTTGACAATATCA+TGG + tig0025447:18062-18081 Msa1422730:intron 25.0%
! AACACACCAAGAAAAAAAAA+AGG - tig0025447:18320-18339 None:intergenic 25.0%
!! AATATTTGCTTCTAAAAGCA+TGG - tig0025447:18674-18693 None:intergenic 25.0%
!!! TTGGAAACAAAAGTACTTTT+TGG + tig0025447:18698-18717 Msa1422730:intron 25.0%
!!! TATTTTGGTGTTCTGTTTAT+AGG + tig0025447:18752-18771 Msa1422730:intron 25.0%
!! TTATGTGTTTGAATCTTTTG+TGG + tig0025447:18776-18795 Msa1422730:intron 25.0%
! AGAAAATAAAGCGAAAAAGA+GGG - tig0025447:18964-18983 None:intergenic 25.0%
! AAGAAAATAAAGCGAAAAAG+AGG - tig0025447:18965-18984 None:intergenic 25.0%
!! ATTTTTCCTCATAGTGATTT+TGG - tig0025447:19023-19042 None:intergenic 25.0%
!! AGGAAAAATCTACATTTTGA+AGG + tig0025447:19034-19053 Msa1422730:intron 25.0%
! TCTAACTTGAAACTAGAATA+TGG + tig0025447:19355-19374 Msa1422730:intron 25.0%
!!! ATATTCAACTCACTTTTACA+TGG + tig0025447:19416-19435 Msa1422730:intron 25.0%
! ATTAGCTAAGAAAATTGCTT+TGG - tig0025447:19548-19567 None:intergenic 25.0%
!! AAGCAATTTTCTTAGCTAAT+TGG + tig0025447:19548-19567 Msa1422730:intron 25.0%
! ATTTGACAATTTCACAGATT+TGG + tig0025447:19571-19590 Msa1422730:intron 25.0%
! GAAATTATGGACATAGTTTA+AGG + tig0025447:20270-20289 Msa1422730:CDS 25.0%
! GCCTAAATAAAATAAGTGAA+TGG - tig0025447:20526-20545 None:intergenic 25.0%
!! ATGCTAACTTCTTTTCTTTT+GGG - tig0025447:20594-20613 None:intergenic 25.0%
!! TATGCTAACTTCTTTTCTTT+TGG - tig0025447:20595-20614 None:intergenic 25.0%
! AAAAGAAAAGAAGTTAGCAT+AGG + tig0025447:20594-20613 Msa1422730:three_prime_UTR 25.0%
! AAAGAAAAGAAGTTAGCATA+GGG + tig0025447:20595-20614 Msa1422730:three_prime_UTR 25.0%
! TAAAACCAAAACACAATACA+TGG - tig0025447:20687-20706 None:intergenic 25.0%
!!! GGTTTTATCATTTGTTTGTT+AGG + tig0025447:20700-20719 Msa1422730:three_prime_UTR 25.0%
! ATTGATTGAAACAAAAGCAA+AGG - tig0025447:20894-20913 None:intergenic 25.0%
ATCTAATTGTGCAAAGCTTA+TGG - tig0025447:16789-16808 None:intergenic 30.0%
AGATTATGTTGAGTACTACA+AGG - tig0025447:16948-16967 None:intergenic 30.0%
TCACAAAGAAACAAACAGTA+AGG + tig0025447:17030-17049 Msa1422730:five_prime_UTR 30.0%
TACACATTCAACAATGTTAC+AGG - tig0025447:17167-17186 None:intergenic 30.0%
AAAGCAAAAAGAAACACTTG+TGG - tig0025447:17225-17244 None:intergenic 30.0%
! TCAACACTGCAAAGTTTTTA+AGG + tig0025447:17309-17328 Msa1422730:five_prime_UTR 30.0%
TCAAGAAGAAGATGAAGAAA+AGG + tig0025447:17504-17523 Msa1422730:CDS 30.0%
TGTAGAAGAGAGAAAAAAGA+AGG + tig0025447:17658-17677 Msa1422730:intron 30.0%
TAAGAGTTCAAATCATGCTA+GGG + tig0025447:17887-17906 Msa1422730:CDS 30.0%
ATCTTCAAGACATTTCAGAT+AGG + tig0025447:18569-18588 Msa1422730:CDS 30.0%
! TAGTAAGTCATATGTTGCTT+TGG + tig0025447:18599-18618 Msa1422730:intron 30.0%
!!! CTTTTTGGTTAGAAAAGCAA+AGG + tig0025447:18713-18732 Msa1422730:intron 30.0%
AAGTAGTATTGCAGATGATT+GGG + tig0025447:18822-18841 Msa1422730:CDS 30.0%
! TTGTTCTAAGTCTGCATATT+TGG + tig0025447:18983-19002 Msa1422730:intron 30.0%
!!! GAATTTTTTGGATCTTCTCA+AGG + tig0025447:19183-19202 Msa1422730:intron 30.0%
TTCAATGTAATCACTAGTGT+CGG - tig0025447:19245-19264 None:intergenic 30.0%
! TTTGCCTCAACAATGATTTT+TGG + tig0025447:19382-19401 Msa1422730:intron 30.0%
GATACCAAAAATCATTGTTG+AGG - tig0025447:19389-19408 None:intergenic 30.0%
!! AAGAAAATTGCTTTGGTGTT+TGG - tig0025447:19541-19560 None:intergenic 30.0%
ATTTCACAGATTTGGAGAAA+TGG + tig0025447:19579-19598 Msa1422730:intron 30.0%
TCTCAACCTTATTCATATCT+AGG + tig0025447:19747-19766 Msa1422730:CDS 30.0%
TTTGCTCCTAGATATGAATA+AGG - tig0025447:19756-19775 None:intergenic 30.0%
AGGTCTAATTACCATTACAA+TGG + tig0025447:20002-20021 Msa1422730:CDS 30.0%
GGTCTAATTACCATTACAAT+GGG + tig0025447:20003-20022 Msa1422730:CDS 30.0%
GAACTTGTACCAAAATAGTA+GGG + tig0025447:20025-20044 Msa1422730:CDS 30.0%
AACTTGTACCAAAATAGTAG+GGG + tig0025447:20026-20045 Msa1422730:CDS 30.0%
AGTACAACAAGAAGAGTAAT+TGG - tig0025447:20354-20373 None:intergenic 30.0%
! TGCTAACTTCTTTTCTTTTG+GGG - tig0025447:20593-20612 None:intergenic 30.0%
GAACCTAAAAATCTGATGTT+AGG + tig0025447:20619-20638 Msa1422730:three_prime_UTR 30.0%
! ACTCCTAACATCAGATTTTT+AGG - tig0025447:20625-20644 None:intergenic 30.0%
ACAACAAGTATGTCCTTAAA+TGG + tig0025447:20789-20808 Msa1422730:three_prime_UTR 30.0%
! AGGTATACCTTAGGCTTTTA+GGG - tig0025447:16725-16744 None:intergenic 35.0%
! AAGGTATACCTTAGGCTTTT+AGG - tig0025447:16726-16745 None:intergenic 35.0%
! TATGGATTTTTGTGGCATGA+AGG - tig0025447:16771-16790 None:intergenic 35.0%
! GCAAAGCTTATGGATTTTTG+TGG - tig0025447:16779-16798 None:intergenic 35.0%
ATATAATGGTAGTCAGAGAC+AGG - tig0025447:16890-16909 None:intergenic 35.0%
AAGAAACACTTGTGGATCTT+TGG - tig0025447:17217-17236 None:intergenic 35.0%
AGAAGAGAAGGAGAAAAACA+AGG - tig0025447:17248-17267 None:intergenic 35.0%
! AAGTTTTACATGGGGAAGAA+AGG + tig0025447:17388-17407 Msa1422730:exon 35.0%
AGAAGAGAGAAAAAAGAAGG+TGG + tig0025447:17661-17680 Msa1422730:intron 35.0%
TTCACTGTTTCATGATTCAC+TGG - tig0025447:17699-17718 None:intergenic 35.0%
ACATCATGCTTCAACTTTGT+TGG - tig0025447:17741-17760 None:intergenic 35.0%
TGTTGTTAATGCTACAAGTG+TGG + tig0025447:17758-17777 Msa1422730:CDS 35.0%
CTAAGAGTTCAAATCATGCT+AGG + tig0025447:17886-17905 Msa1422730:CDS 35.0%
! GTGGTTCAAACTTCTTTTAG+AGG + tig0025447:17927-17946 Msa1422730:CDS 35.0%
AACAGATCTAATTGCCTGTT+TGG + tig0025447:17969-17988 Msa1422730:intron 35.0%
AACTTGCAGTAATTCCAAAC+AGG - tig0025447:17986-18005 None:intergenic 35.0%
! GTGTAGTTTTTGCCAAATCA+CGG - tig0025447:18209-18228 None:intergenic 35.0%
TGTGATTTCGTCAAATTCAC+TGG + tig0025447:18259-18278 Msa1422730:intron 35.0%
GTGATTTCGTCAAATTCACT+GGG + tig0025447:18260-18279 Msa1422730:intron 35.0%
TTGTTATGACAGTCAAGAGA+AGG + tig0025447:18799-18818 Msa1422730:intron 35.0%
GAAGTAGTATTGCAGATGAT+TGG + tig0025447:18821-18840 Msa1422730:CDS 35.0%
TTAACTTCTTCAACTGTGTG+TGG - tig0025447:18856-18875 None:intergenic 35.0%
ACACACAGTTGAAGAAGTTA+AGG + tig0025447:18855-18874 Msa1422730:CDS 35.0%
TTCTTTCCTTTGTTGCAACA+TGG - tig0025447:18881-18900 None:intergenic 35.0%
AACCTGTTGTGAAAATGCTT+GGG - tig0025447:18932-18951 None:intergenic 35.0%
AAACCTGTTGTGAAAATGCT+TGG - tig0025447:18933-18952 None:intergenic 35.0%
AGTTTGCCAAAATCACTATG+AGG + tig0025447:19014-19033 Msa1422730:intron 35.0%
! TGTATGTGTGTTTCCACTTT+TGG + tig0025447:19122-19141 Msa1422730:intron 35.0%
TCAAGGAGAATTGAGTTTGA+AGG + tig0025447:19200-19219 Msa1422730:intron 35.0%
AAGTTAGAACCTATGAAGCA+CGG - tig0025447:19343-19362 None:intergenic 35.0%
ATGAATTGACTGTGTAACAC+TGG - tig0025447:19491-19510 None:intergenic 35.0%
AATGGTAGGACATCATCAAT+TGG + tig0025447:19597-19616 Msa1422730:CDS 35.0%
AACTTGAAGAGAGACCAAAA+TGG + tig0025447:19631-19650 Msa1422730:CDS 35.0%
! ATCCATCTATCTAGCCATTT+TGG - tig0025447:19648-19667 None:intergenic 35.0%
AAAATGGCTAGATAGATGGA+TGG + tig0025447:19647-19666 Msa1422730:CDS 35.0%
TACAAAACCATGGGAGAATA+GGG + tig0025447:19671-19690 Msa1422730:CDS 35.0%
AAGAGACTCTATTAAGACTG+TGG + tig0025447:19713-19732 Msa1422730:CDS 35.0%
!! ATCCATTTTGGTGTGATCTA+TGG - tig0025447:19854-19873 None:intergenic 35.0%
! TGAATAGGTCTTGATTTTGC+TGG - tig0025447:19909-19928 None:intergenic 35.0%
AATCAAGACCTATTCAAGTC+CGG + tig0025447:19913-19932 Msa1422730:CDS 35.0%
TGGTAATTAGACCTTAAGCT+TGG - tig0025447:19996-20015 None:intergenic 35.0%
GGAACTTGTACCAAAATAGT+AGG + tig0025447:20024-20043 Msa1422730:CDS 35.0%
! AAGCCTTTTCTTTACTGATC+CGG - tig0025447:20204-20223 None:intergenic 35.0%
! GGAGTTGGTTATGGAAATTA+TGG + tig0025447:20257-20276 Msa1422730:CDS 35.0%
ACATTCACACTCTTGAAACT+TGG - tig0025447:20299-20318 None:intergenic 35.0%
AATGTAGCAGCATCACATTA+TGG + tig0025447:20314-20333 Msa1422730:CDS 35.0%
TCAACTACACTCAGTTTCAA+CGG + tig0025447:20443-20462 Msa1422730:three_prime_UTR 35.0%
GGTTACAACTATGACACATT+AGG + tig0025447:20490-20509 Msa1422730:three_prime_UTR 35.0%
!!! CTTTTCTTTTGGGGTGAAAA+GGG - tig0025447:20584-20603 None:intergenic 35.0%
!!! TCTTTTCTTTTGGGGTGAAA+AGG - tig0025447:20585-20604 None:intergenic 35.0%
! GAGAACCATGTATTGTGTTT+TGG + tig0025447:20679-20698 Msa1422730:three_prime_UTR 35.0%
!! TAAGGACATACTTGTTGTGT+AGG - tig0025447:20787-20806 None:intergenic 35.0%
!!! TTAGGCTTTTAGGGTCTGTT+TGG - tig0025447:16716-16735 None:intergenic 40.0%
AAACAGACCCTAAAAGCCTA+AGG + tig0025447:16715-16734 Msa1422730:five_prime_UTR 40.0%
! AGTGTCAGAAGGTATACCTT+AGG - tig0025447:16734-16753 None:intergenic 40.0%
CAGAAAGGTTCAACTTGGTT+GGG - tig0025447:17286-17305 None:intergenic 40.0%
ACAGAAAGGTTCAACTTGGT+TGG - tig0025447:17287-17306 None:intergenic 40.0%
GAAGACAGAAAGGTTCAACT+TGG - tig0025447:17291-17310 None:intergenic 40.0%
! TTGCAGTGTTGAAGACAGAA+AGG - tig0025447:17301-17320 None:intergenic 40.0%
GGAAGAAAGGTAGTAGTTCA+TGG + tig0025447:17401-17420 Msa1422730:CDS 40.0%
TTACAGTGACACCGTGATTT+CGG - tig0025447:18011-18030 None:intergenic 40.0%
TCCAAAATCATTGTGGCCAT+TGG - tig0025447:18152-18171 None:intergenic 40.0%
! TGGCCACAATGATTTTGGAT+CGG + tig0025447:18153-18172 Msa1422730:intron 40.0%
! TTTTGGATCGGTGGTGAATT+TGG + tig0025447:18165-18184 Msa1422730:intron 40.0%
CAAATCACGGTTGAACATTG+TGG - tig0025447:18196-18215 None:intergenic 40.0%
CACAATGTTCAACCGTGATT+TGG + tig0025447:18194-18213 Msa1422730:intron 40.0%
TTGACGAAATCACAGTGACA+CGG - tig0025447:18253-18272 None:intergenic 40.0%
TTGGTGTGTTTGAACCTTGA+AGG + tig0025447:18330-18349 Msa1422730:intron 40.0%
! AAGTTGCAAGCTTTAGTGAG+AGG + tig0025447:18388-18407 Msa1422730:CDS 40.0%
TAGTGAGAGGTCACAATGTT+AGG + tig0025447:18401-18420 Msa1422730:CDS 40.0%
AGCAAGCAAAGATGACACTT+AGG + tig0025447:18425-18444 Msa1422730:CDS 40.0%
AAGCAAGAGTTCTTGACCAA+AGG + tig0025447:18473-18492 Msa1422730:CDS 40.0%
GAGTTCTTGACCAAAGGATA+AGG + tig0025447:18479-18498 Msa1422730:CDS 40.0%
GTGAAGTAGACCTTATCCTT+TGG - tig0025447:18492-18511 None:intergenic 40.0%
ATAAGGTCTACTTCACACGA+AGG + tig0025447:18496-18515 Msa1422730:CDS 40.0%
! AACACTAGCAGTGTCACTAA+AGG - tig0025447:18536-18555 None:intergenic 40.0%
TTAGTGACACTGCTAGTGTT+TGG + tig0025447:18536-18555 Msa1422730:CDS 40.0%
TAGTGACACTGCTAGTGTTT+GGG + tig0025447:18537-18556 Msa1422730:CDS 40.0%
TTAAGGCCATGTTGCAACAA+AGG + tig0025447:18872-18891 Msa1422730:CDS 40.0%
GAAAGAAGCAGCAATGAAGA+GGG + tig0025447:18894-18913 Msa1422730:CDS 40.0%
!! ATGTGTGTTTCCACTTTTGG+AGG + tig0025447:19125-19144 Msa1422730:intron 40.0%
CAATTTCACTCCTCCAAAAG+TGG - tig0025447:19138-19157 None:intergenic 40.0%
CACAGATTTGGAGAAATGGT+AGG + tig0025447:19583-19602 Msa1422730:intron 40.0%
GACCAAAATGGCTAGATAGA+TGG + tig0025447:19643-19662 Msa1422730:CDS 40.0%
ATGGATGGCTACAAAACCAT+GGG + tig0025447:19662-19681 Msa1422730:CDS 40.0%
CTACAAAACCATGGGAGAAT+AGG + tig0025447:19670-19689 Msa1422730:CDS 40.0%
ACAAAACCATGGGAGAATAG+GGG + tig0025447:19672-19691 Msa1422730:CDS 40.0%
!! TTTTGGTGTGATCTATGGAG+CGG - tig0025447:19849-19868 None:intergenic 40.0%
CTCCATAGATCACACCAAAA+TGG + tig0025447:19849-19868 Msa1422730:CDS 40.0%
!! AGTGATGGATTGGTTGTGAA+CGG - tig0025447:19877-19896 None:intergenic 40.0%
TATCTTCTCGAACACAACGA+GGG - tig0025447:19947-19966 None:intergenic 40.0%
GGTACAAGTTCCCATTGTAA+TGG - tig0025447:20016-20035 None:intergenic 40.0%
TTGTACCAAAATAGTAGGGG+TGG + tig0025447:20029-20048 Msa1422730:CDS 40.0%
! TTGTTCCACCCCTACTATTT+TGG - tig0025447:20037-20056 None:intergenic 40.0%
TCGGTTGCTTGCATGTAATT+CGG - tig0025447:20110-20129 None:intergenic 40.0%
GATCCGGATCAGTAAAGAAA+AGG + tig0025447:20198-20217 Msa1422730:CDS 40.0%
CATTATAAGGATCAGGAGCA+GGG - tig0025447:20232-20251 None:intergenic 40.0%
ACATTATAAGGATCAGGAGC+AGG - tig0025447:20233-20252 None:intergenic 40.0%
GCTCCTGATCCTTATAATGT+AGG + tig0025447:20233-20252 Msa1422730:CDS 40.0%
CCTCCTACATTATAAGGATC+AGG - tig0025447:20239-20258 None:intergenic 40.0%
CCTGATCCTTATAATGTAGG+AGG + tig0025447:20236-20255 Msa1422730:CDS 40.0%
CCAACTCCTCCTACATTATA+AGG - tig0025447:20245-20264 None:intergenic 40.0%
CCTTATAATGTAGGAGGAGT+TGG + tig0025447:20242-20261 Msa1422730:CDS 40.0%
! AATGTAGGAGGAGTTGGTTA+TGG + tig0025447:20248-20267 Msa1422730:CDS 40.0%
AGCAGCATCACATTATGGAT+TGG + tig0025447:20319-20338 Msa1422730:CDS 40.0%
! TCTTGTTGTACTGAGAGTCT+CGG + tig0025447:20362-20381 Msa1422730:CDS 40.0%
GCAAGTACTATGTTGTTGCT+AGG + tig0025447:20651-20670 Msa1422730:three_prime_UTR 40.0%
TAGGATGCCAAGATAAATGC+TGG + tig0025447:20719-20738 Msa1422730:three_prime_UTR 40.0%
CATATGGCCAGCATTTATCT+TGG - tig0025447:20729-20748 None:intergenic 40.0%
TAAATGCTGGCCATATGTTC+TGG + tig0025447:20732-20751 Msa1422730:three_prime_UTR 40.0%
GTGACAAGACAGTCCATTTA+AGG - tig0025447:20805-20824 None:intergenic 40.0%
! GGATTTTTGTGGCATGAAGG+TGG - tig0025447:16768-16787 None:intergenic 45.0%
TGTGTTTGTGAGTGAGTGAG+AGG - tig0025447:16866-16885 None:intergenic 45.0%
AAAGGTTCAACTTGGTTGGG+TGG - tig0025447:17283-17302 None:intergenic 45.0%
ATGCTACAAGTGTGGCTTCA+AGG + tig0025447:17766-17785 Msa1422730:CDS 45.0%
TGAGAAACATGCTCTTGCTG+TGG + tig0025447:17800-17819 Msa1422730:CDS 45.0%
CTGCAAGTTTGCCGAAATCA+CGG + tig0025447:17997-18016 Msa1422730:intron 45.0%
ACTCATTGTGATCCTGCCAA+TGG + tig0025447:18133-18152 Msa1422730:intron 45.0%
AAATCATTGTGGCCATTGGC+AGG - tig0025447:18148-18167 None:intergenic 45.0%
! GCCAATGGCCACAATGATTT+TGG + tig0025447:18148-18167 Msa1422730:intron 45.0%
CCACCGATCCAAAATCATTG+TGG - tig0025447:18159-18178 None:intergenic 45.0%
! CCACAATGATTTTGGATCGG+TGG + tig0025447:18156-18175 Msa1422730:intron 45.0%
AAGTGCTCTTCTTGCCTTCA+AGG - tig0025447:18347-18366 None:intergenic 45.0%
GGAAAGAAGCAGCAATGAAG+AGG + tig0025447:18893-18912 Msa1422730:CDS 45.0%
! GTCCCAAGCATTTTCACAAC+AGG + tig0025447:18927-18946 Msa1422730:CDS 45.0%
CGATGAGTTCGACAAAGTCT+AGG - tig0025447:19078-19097 None:intergenic 45.0%
!! GTAATCACTAGTGTCGGTGT+CGG - tig0025447:19239-19258 None:intergenic 45.0%
TATCTGTGTCCGTGCTTCAT+AGG + tig0025447:19331-19350 Msa1422730:intron 45.0%
GATGGATGGCTACAAAACCA+TGG + tig0025447:19661-19680 Msa1422730:CDS 45.0%
ATCCACATCACCAACCACAA+AGG + tig0025447:19808-19827 Msa1422730:CDS 45.0%
CAATGGAATGCCTTTGTGGT+TGG - tig0025447:19821-19840 None:intergenic 45.0%
GAAGCAATGGAATGCCTTTG+TGG - tig0025447:19825-19844 None:intergenic 45.0%
! GGTTGTGAACGGATCCATTT+TGG - tig0025447:19866-19885 None:intergenic 45.0%
! GGTCTTGATTTTGCTGGAGA+CGG - tig0025447:19903-19922 None:intergenic 45.0%
CTATCTTCTCGAACACAACG+AGG - tig0025447:19948-19967 None:intergenic 45.0%
!! CCTTAAGCTTGGTGTTTGAG+AGG - tig0025447:19985-20004 None:intergenic 45.0%
CCTCTCAAACACCAAGCTTA+AGG + tig0025447:19982-20001 Msa1422730:CDS 45.0%
AGTAGGGGTGGAACAAGTAA+TGG + tig0025447:20041-20060 Msa1422730:CDS 45.0%
AATTCGAGCCTTTGCAGACT+CGG - tig0025447:20129-20148 None:intergenic 45.0%
AGTCTGCAAAGGCTCGAATT+CGG + tig0025447:20129-20148 Msa1422730:CDS 45.0%
ATTGTTCAACACCCCAAGTG+AGG - tig0025447:20762-20781 None:intergenic 45.0%
!!! TTTACATAATAATAAAATTT+TGG + tig0025447:16812-16831 Msa1422730:five_prime_UTR 5.0%
!!! ATTATATATTCATTTTTTTT+TGG + tig0025447:17063-17082 Msa1422730:five_prime_UTR 5.0%
GTTTGTGAGTGAGTGAGAGG+AGG - tig0025447:16863-16882 None:intergenic 50.0%
TGTGGCTTCAAGGACAGATC+AGG + tig0025447:17776-17795 Msa1422730:CDS 50.0%
! ACATGCTCTTGCTGTGGCTA+TGG + tig0025447:17806-17825 Msa1422730:CDS 50.0%
AAGCTGCTGTGGAAGTTGCA+AGG + tig0025447:17859-17878 Msa1422730:CDS 50.0%
! GGAGCATTATGCTGCTGTTG+TGG + tig0025447:17908-17927 Msa1422730:CDS 50.0%
!! AAGAGCACTTCGTGCGCTTA+AGG + tig0025447:18357-18376 Msa1422730:CDS 50.0%
! AGAGCACTTCGTGCGCTTAA+GGG + tig0025447:18358-18377 Msa1422730:CDS 50.0%
! CTGCTAGTGTTTGGGATTCG+CGG + tig0025447:18545-18564 Msa1422730:CDS 50.0%
! TTGAGTTTGAAGGCGTGTCC+CGG + tig0025447:19210-19229 Msa1422730:intron 50.0%
CTGACACAAACACGTCGACA+CGG - tig0025447:19293-19312 None:intergenic 50.0%
GTTTGTGTCAGTGTCGTGTC+CGG + tig0025447:19302-19321 Msa1422730:intron 50.0%
ACGGACACAGATACGTACAC+CGG - tig0025447:19324-19343 None:intergenic 50.0%
!! TGCCTTTGTGGTTGGTGATG+TGG - tig0025447:19813-19832 None:intergenic 50.0%
TCTATGGAGCGGTGAAGCAA+TGG - tig0025447:19838-19857 None:intergenic 50.0%
!! GAGACGGAGTAGTGATGGAT+TGG - tig0025447:19887-19906 None:intergenic 50.0%
CTTGACGACCGGACTTGAAT+AGG - tig0025447:19924-19943 None:intergenic 50.0%
! TTGGTGTTTGAGAGGTGTGG+TGG - tig0025447:19977-19996 None:intergenic 50.0%
!! AGCTTGGTGTTTGAGAGGTG+TGG - tig0025447:19980-19999 None:intergenic 50.0%
ACACCAGAGAGGGATAGATC+CGG + tig0025447:20182-20201 Msa1422730:CDS 50.0%
TGTTGTACTGAGAGTCTCGG+TGG + tig0025447:20365-20384 Msa1422730:CDS 50.0%
GAGGTTTCACCTTCGTCAAC+CGG + tig0025447:20389-20408 Msa1422730:CDS 50.0%
CTCATCTCAACCACCGTCTA+AGG - tig0025447:20418-20437 None:intergenic 50.0%
! TGCAACGACTGCGGAGTATT+CGG + tig0025447:20469-20488 Msa1422730:three_prime_UTR 50.0%
GTGAGGTAGGCCAGAACATA+TGG - tig0025447:20745-20764 None:intergenic 50.0%
ATGTTCTGGCCTACCTCACT+TGG + tig0025447:20746-20765 Msa1422730:three_prime_UTR 50.0%
TGTTCTGGCCTACCTCACTT+GGG + tig0025447:20747-20766 Msa1422730:three_prime_UTR 50.0%
TTCAACACCCCAAGTGAGGT+AGG - tig0025447:20758-20777 None:intergenic 50.0%
!! ACAGCAGTGGGAGTGTCAGA+AGG - tig0025447:16745-16764 None:intergenic 55.0%
GCATGAAGGTGGACAGCAGT+GGG - tig0025447:16757-16776 None:intergenic 55.0%
TGAGTGAGTGAGAGGAGGAG+GGG - tig0025447:16858-16877 None:intergenic 55.0%
GTGAGTGAGTGAGAGGAGGA+GGG - tig0025447:16859-16878 None:intergenic 55.0%
TGTGAGTGAGTGAGAGGAGG+AGG - tig0025447:16860-16879 None:intergenic 55.0%
TGTGGCTATGGCTACAGCTG+AGG + tig0025447:17818-17837 Msa1422730:CDS 55.0%
AGCTACTGCTCAAGCTGCTG+TGG + tig0025447:17848-17867 Msa1422730:CDS 55.0%
GAGCACTTCGTGCGCTTAAG+GGG + tig0025447:18359-18378 Msa1422730:CDS 55.0%
GCTCTTCCCCTATTCTCCCA+TGG - tig0025447:19681-19700 None:intergenic 55.0%
TGCTGGAGACGGAGTAGTGA+TGG - tig0025447:19892-19911 None:intergenic 55.0%
GCAAGCAACCGAGTCTGCAA+AGG + tig0025447:20118-20137 Msa1422730:CDS 55.0%
AAAGGCCATCGACACCAGAG+AGG + tig0025447:20171-20190 Msa1422730:CDS 55.0%
AAGGCCATCGACACCAGAGA+GGG + tig0025447:20172-20191 Msa1422730:CDS 55.0%
! CTATCCCTCTCTGGTGTCGA+TGG - tig0025447:20179-20198 None:intergenic 55.0%
GATCCGGATCTATCCCTCTC+TGG - tig0025447:20188-20207 None:intergenic 55.0%
! CTAAGGTCACCGGTTGACGA+AGG - tig0025447:20401-20420 None:intergenic 55.0%
CGTCAACCGGTGACCTTAGA+CGG + tig0025447:20402-20421 Msa1422730:CDS 55.0%
! CAACCACCGTCTAAGGTCAC+CGG - tig0025447:20411-20430 None:intergenic 55.0%
GTTCTGGCCTACCTCACTTG+GGG + tig0025447:20748-20767 Msa1422730:three_prime_UTR 55.0%
GGCATGAAGGTGGACAGCAG+TGG - tig0025447:16758-16777 None:intergenic 60.0%
GAGTGAGTGAGAGGAGGAGG+GGG - tig0025447:16857-16876 None:intergenic 60.0%
!! TAGTGTCGGTGTCGGACACC+GGG - tig0025447:19231-19250 None:intergenic 60.0%
!! CTAGTGTCGGTGTCGGACAC+CGG - tig0025447:19232-19251 None:intergenic 60.0%
CACAACGAGGGCTTGACGAC+CGG - tig0025447:19935-19954 None:intergenic 60.0%
!! GGTGTCGATGGCCTTTGCCT+TGG - tig0025447:20167-20186 None:intergenic 60.0%
TACTGAGAGTCTCGGTGGCG+AGG + tig0025447:20370-20389 Msa1422730:CDS 60.0%
CAACCGGTGACCTTAGACGG+TGG + tig0025447:20405-20424 Msa1422730:CDS 60.0%
CAACGGCTCTGCAACGACTG+CGG + tig0025447:20460-20479 Msa1422730:three_prime_UTR 60.0%
TTCGGTCGCAGAGTGCGCCA+AGG + tig0025447:20147-20166 Msa1422730:CDS 65.0%
CGCAGAGTGCGCCAAGGCAA+AGG + tig0025447:20153-20172 Msa1422730:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0025447 gene 16695 20927 16695 ID=Msa1422730;Name=Msa1422730
tig0025447 mRNA 16695 20927 16695 ID=Msa1422730-mRNA-1;Parent=Msa1422730;Name=Msa1422730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=702|1|1|1|1|1|5|491|552
tig0025447 exon 16695 17525 16695 ID=Msa1422730-mRNA-1:exon:2750;Parent=Msa1422730-mRNA-1
tig0025447 exon 17663 17956 17663 ID=Msa1422730-mRNA-1:exon:2751;Parent=Msa1422730-mRNA-1
tig0025447 exon 18352 18600 18352 ID=Msa1422730-mRNA-1:exon:2752;Parent=Msa1422730-mRNA-1
tig0025447 exon 18811 18948 18811 ID=Msa1422730-mRNA-1:exon:2753;Parent=Msa1422730-mRNA-1
tig0025447 exon 19588 20927 19588 ID=Msa1422730-mRNA-1:exon:2754;Parent=Msa1422730-mRNA-1
tig0025447 five_prime_UTR 16695 17396 16695 ID=Msa1422730-mRNA-1:five_prime_utr;Parent=Msa1422730-mRNA-1
tig0025447 CDS 17397 17525 17397 ID=Msa1422730-mRNA-1:cds;Parent=Msa1422730-mRNA-1
tig0025447 CDS 17663 17956 17663 ID=Msa1422730-mRNA-1:cds;Parent=Msa1422730-mRNA-1
tig0025447 CDS 18352 18600 18352 ID=Msa1422730-mRNA-1:cds;Parent=Msa1422730-mRNA-1
tig0025447 CDS 18811 18948 18811 ID=Msa1422730-mRNA-1:cds;Parent=Msa1422730-mRNA-1
tig0025447 CDS 19588 20436 19588 ID=Msa1422730-mRNA-1:cds;Parent=Msa1422730-mRNA-1
tig0025447 three_prime_UTR 20437 20927 20437 ID=Msa1422730-mRNA-1:three_prime_utr;Parent=Msa1422730-mRNA-1
Gene Sequence

>Msa1422730

ATGGGGAAGAAAGGTAGTAGTTCATGGTTAACTGCTGTGAAAAGAGCATTCAGATCTCCAACAAAAGATAGTGAAAAAAGAAGTGGTAGAAGAAGAGAAGATTGTGATCAAGAAGAAGATGAAGAAAAGAAGAGAGAAAAAAGAAGGTGGATTTTTAGGAAACCAGTGAATCATGAAACAGTGAATAATACACAACAAAGTACACCAACAAAGTTGAAGCATGATGTTGTTAATGCTACAAGTGTGGCTTCAAGGACAGATCAGGATGAGAAACATGCTCTTGCTGTGGCTATGGCTACAGCTGAGGCAGCAAGAGCTACTGCTCAAGCTGCTGTGGAAGTTGCAAGGTTGACTAAGAGTTCAAATCATGCTAGGGAGCATTATGCTGCTGTTGTGGTTCAAACTTCTTTTAGAGGTTATTTGGCAAGAAGAGCACTTCGTGCGCTTAAGGGGTTAGTGAAGTTGCAAGCTTTAGTGAGAGGTCACAATGTTAGGAAGCAAGCAAAGATGACACTTAGGTGCATGCAAGCTCTTGTTAGAGTACAAGCAAGAGTTCTTGACCAAAGGATAAGGTCTACTTCACACGAAGGAAGTAGAAAATCAACCTTTAGTGACACTGCTAGTGTTTGGGATTCGCGGTATCTTCAAGACATTTCAGATAGGAAATCACTATCAAGAGAAGGAAGTAGTATTGCAGATGATTGGGATGATCGTCCACACACAGTTGAAGAAGTTAAGGCCATGTTGCAACAAAGGAAAGAAGCAGCAATGAAGAGGGAAAAGTCATTGTCCCAAGCATTTTCACAACAGATTTGGAGAAATGGTAGGACATCATCAATTGGAAATGAAGATGAACTTGAAGAGAGACCAAAATGGCTAGATAGATGGATGGCTACAAAACCATGGGAGAATAGGGGAAGAGCTTCAACTGATCAAAGAGACTCTATTAAGACTGTGGAAGTCGACACATCTCAACCTTATTCATATCTAGGAGCAAATTACAGAAGATCACATCCAAATTATCAATATAATCCACATCACCAACCACAAAGGCATTCCATTGCTTCACCGCTCCATAGATCACACCAAAATGGATCCGTTCACAACCAATCCATCACTACTCCGTCTCCAGCAAAATCAAGACCTATTCAAGTCCGGTCGTCAAGCCCTCGTTGTGTTCGAGAAGATAGAAGCTACCACCACACCTCTCAAACACCAAGCTTAAGGTCTAATTACCATTACAATGGGAACTTGTACCAAAATAGTAGGGGTGGAACAAGTAATGGTAGTAGTAACAGTATTGCTTCTACTACTGCTTCAGCTACATTGCCGAATTACATGCAAGCAACCGAGTCTGCAAAGGCTCGAATTCGGTCGCAGAGTGCGCCAAGGCAAAGGCCATCGACACCAGAGAGGGATAGATCCGGATCAGTAAAGAAAAGGCTTTCTTTCCCTGCTCCTGATCCTTATAATGTAGGAGGAGTTGGTTATGGAAATTATGGACATAGTTTAAGGAGTCCAAGTTTCAAGAGTGTGAATGTAGCAGCATCACATTATGGATTGGAGCAAAATTCCAATTACTCTTCTTGTTGTACTGAGAGTCTCGGTGGCGAGGTTTCACCTTCGTCAACCGGTGACCTTAGACGGTGGTTGAGATGA
Protein sequence

>Msa1422730

MGKKGSSSWLTAVKRAFRSPTKDSEKRSGRRREDCDQEEDEEKKREKRRWIFRKPVNHETVNNTQQSTPTKLKHDVVNATSVASRTDQDEKHALAVAMATAEAARATAQAAVEVARLTKSSNHAREHYAAVVVQTSFRGYLARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALVRVQARVLDQRIRSTSHEGSRKSTFSDTASVWDSRYLQDISDRKSLSREGSSIADDWDDRPHTVEEVKAMLQQRKEAAMKREKSLSQAFSQQIWRNGRTSSIGNEDELEERPKWLDRWMATKPWENRGRASTDQRDSIKTVEVDTSQPYSYLGANYRRSHPNYQYNPHHQPQRHSIASPLHRSHQNGSVHNQSITTPSPAKSRPIQVRSSSPRCVREDRSYHHTSQTPSLRSNYHYNGNLYQNSRGGTSNGSSNSIASTTASATLPNYMQATESAKARIRSQSAPRQRPSTPERDRSGSVKKRLSFPAPDPYNVGGVGYGNYGHSLRSPSFKSVNVAASHYGLEQNSNYSSCCTESLGGEVSPSSTGDLRRWLR*