AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1236010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236010 MtrunA17_Chr8g0346021 99.275 552 4 0 1 552 1 552 0.0 1135
Msa1236010 MtrunA17_Chr6g0462191 34.805 385 179 16 112 486 89 411 2.85e-36 140
Msa1236010 MtrunA17_Chr1g0181581 28.906 384 215 9 112 486 101 435 1.51e-34 135
Msa1236010 MtrunA17_Chr7g0263731 31.486 397 209 13 107 486 88 438 1.78e-34 135
Msa1236010 MtrunA17_Chr4g0025441 45.545 202 89 7 126 323 95 279 2.55e-34 134
Msa1236010 MtrunA17_Chr1g0200161 38.860 193 100 4 126 314 140 318 4.46e-33 132
Msa1236010 MtrunA17_Chr4g0045641 27.652 528 283 18 1 485 1 472 2.49e-32 130
Msa1236010 MtrunA17_Chr3g0126901 35.754 358 165 10 1 333 1 318 3.76e-32 128
Msa1236010 MtrunA17_Chr1g0146931 43.961 207 97 6 116 305 111 315 1.37e-28 119
Msa1236010 MtrunA17_Chr6g0472551 30.411 365 169 11 129 477 119 414 7.95e-27 113
Msa1236010 MtrunA17_Chr3g0135851 40.933 193 88 4 124 305 88 265 8.46e-27 113
Msa1236010 MtrunA17_Chr4g0020671 29.936 314 149 13 1 300 1 257 6.09e-25 107
Msa1236010 MtrunA17_Chr4g0057321 28.090 445 226 11 30 467 15 372 7.83e-25 107
Msa1236010 MtrunA17_Chr1g0204901 47.531 162 72 4 45 197 48 205 6.42e-24 104
Msa1236010 MtrunA17_Chr1g0203711 31.250 352 156 12 126 466 114 390 1.70e-22 100
Msa1236010 MtrunA17_Chr8g0340761 45.026 191 82 4 44 226 36 211 1.90e-22 99.8
Msa1236010 MtrunA17_Chr2g0283321 32.787 183 103 4 126 305 62 227 5.66e-22 96.7
Msa1236010 MtrunA17_Chr8g0342551 39.474 190 89 4 125 305 88 260 1.60e-20 94.4
Msa1236010 MtrunA17_Chr8g0389781 73.333 60 16 0 126 185 125 184 4.09e-20 93.2
Msa1236010 MtrunA17_Chr1g0166431 44.286 140 56 4 45 179 47 169 1.94e-19 91.3
Msa1236010 MtrunA17_Chr7g0273861 31.148 183 107 4 118 300 116 279 1.76e-18 88.2
Msa1236010 MtrunA17_Chr7g0273951 39.726 146 62 3 47 191 50 170 5.89e-18 85.9
Msa1236010 MtrunA17_Chr8g0371131 60.656 61 24 0 125 185 69 129 2.95e-17 79.0
Msa1236010 MtrunA17_Chr4g0060381 61.905 63 24 0 129 191 102 164 3.75e-17 84.7
Msa1236010 MtrunA17_Chr2g0330921 60.000 70 28 0 124 193 94 163 1.01e-16 82.0
Msa1236010 MtrunA17_Chr2g0330051 39.500 200 77 8 125 303 76 252 4.17e-16 80.5
Msa1236010 MtrunA17_Chr2g0316401 28.934 197 114 8 127 316 74 251 8.27e-16 78.6
Msa1236010 MtrunA17_Chr1g0156121 57.895 57 24 0 129 185 126 182 1.30e-13 73.9
Msa1236010 MtrunA17_Chr7g0215371 52.727 55 26 0 129 183 21 75 4.22e-11 59.7
Msa1236010 MtrunA17_Chr5g0416161 33.846 130 70 4 60 183 56 175 4.26e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236010 AT1G01110.2 57.913 575 171 23 1 552 1 527 0.0 526
Msa1236010 AT4G00820.1 58.007 562 180 18 9 552 11 534 1.62e-180 519
Msa1236010 AT1G01110.1 59.446 397 114 16 170 552 1 364 2.62e-137 402
Msa1236010 AT4G10640.1 36.219 566 204 19 1 552 1 423 2.85e-89 281
Msa1236010 AT3G49380.1 37.959 245 113 5 1 239 1 212 2.83e-37 141
Msa1236010 AT2G26180.1 43.627 204 89 4 119 311 76 264 2.90e-35 137
Msa1236010 AT3G49260.1 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1236010 AT3G49260.4 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1236010 AT3G49260.2 34.316 373 178 14 1 331 1 348 3.86e-35 138
Msa1236010 AT5G03040.1 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1236010 AT5G03040.3 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1236010 AT5G03040.2 28.814 531 280 21 1 503 1 461 9.29e-35 137
Msa1236010 AT3G49260.3 34.501 371 181 14 1 331 1 349 2.89e-34 135
Msa1236010 AT3G22190.3 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1236010 AT3G22190.2 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1236010 AT3G22190.4 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1236010 AT3G22190.1 42.487 193 81 5 123 305 84 256 6.70e-31 125
Msa1236010 AT1G72670.1 37.500 208 104 6 107 305 73 263 1.95e-29 120
Msa1236010 AT3G52290.1 33.228 316 155 13 1 304 1 272 8.87e-29 119
Msa1236010 AT3G59690.2 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa1236010 AT3G59690.1 25.885 537 261 17 1 485 1 452 1.56e-28 119
Msa1236010 AT2G43680.4 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1236010 AT2G43680.2 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1236010 AT2G43680.3 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1236010 AT2G43680.5 26.619 417 225 14 126 539 321 659 2.09e-25 111
Msa1236010 AT2G43680.1 26.619 417 225 14 126 539 322 660 2.18e-25 111
Msa1236010 AT5G13460.1 29.710 276 153 7 38 300 18 265 4.43e-23 102
Msa1236010 AT4G14750.1 68.182 66 21 0 126 191 104 169 4.45e-22 99.0
Msa1236010 AT1G17480.1 36.842 171 81 4 100 270 68 211 9.18e-22 97.8
Msa1236010 AT4G14750.3 68.182 66 21 0 126 191 163 228 1.04e-21 98.6
Msa1236010 AT4G14750.2 68.182 66 21 0 126 191 163 228 1.09e-21 98.6
Msa1236010 AT3G09710.2 30.732 205 118 7 111 303 95 287 1.35e-21 98.2
Msa1236010 AT3G09710.1 30.653 199 112 7 111 303 95 273 1.73e-21 97.8
Msa1236010 AT2G26410.2 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa1236010 AT2G26410.1 30.693 202 116 6 123 318 144 327 5.03e-21 97.1
Msa1236010 AT3G16490.1 65.714 70 24 0 121 190 101 170 1.79e-19 91.3
Msa1236010 AT4G23060.1 28.255 361 165 11 114 467 152 425 9.90e-19 89.7
Msa1236010 AT4G29150.1 44.262 122 57 2 122 241 126 238 2.19e-18 87.8
Msa1236010 AT5G07240.1 67.742 62 20 0 124 185 103 164 5.83e-18 86.7
Msa1236010 AT5G07240.2 67.742 62 20 0 124 185 149 210 7.49e-18 86.7
Msa1236010 AT5G62070.1 70.690 58 17 0 124 181 114 171 7.25e-17 83.2
Msa1236010 AT1G18840.1 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1236010 AT1G18840.3 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1236010 AT1G18840.2 52.113 71 34 0 121 191 103 173 1.25e-16 83.6
Msa1236010 AT1G19870.1 35.556 135 70 2 71 205 173 290 7.50e-16 81.6
Msa1236010 AT2G33990.1 26.601 203 129 4 126 326 46 230 1.58e-15 77.4
Msa1236010 AT1G51960.1 60.000 65 26 0 126 190 99 163 1.93e-15 78.6
Msa1236010 AT1G19870.2 53.333 75 32 1 131 205 36 107 3.36e-15 79.0
Msa1236010 AT3G15050.1 26.630 184 115 4 129 308 52 219 1.33e-14 74.3
Msa1236010 AT1G74690.1 55.385 65 29 0 127 191 113 177 2.72e-14 76.3
Msa1236010 AT3G51380.1 48.235 85 43 1 108 192 19 102 3.14e-14 69.3
Msa1236010 AT1G14380.1 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1236010 AT1G14380.3 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1236010 AT1G14380.4 50.769 65 32 0 129 193 96 160 1.21e-13 74.3
Msa1236010 AT1G14380.6 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1236010 AT1G14380.5 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1236010 AT1G14380.2 50.769 65 32 0 129 193 96 160 1.40e-13 73.9
Msa1236010 AT1G14380.7 50.769 65 32 0 129 193 96 160 1.56e-13 73.9

Find 175 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATAACATGTAATATTT+TGG 0.059916 8_2:+14429501 Msa1236010:three_prime_UTR
TTTACATAATAATAAAATTT+TGG 0.148736 8_2:+14425493 Msa1236010:five_prime_UTR
GTGACAAGACAGTCCATTTA+AGG 0.176423 8_2:-14429481 None:intergenic
GAAATTATGGACATAGTTTA+AGG 0.213922 8_2:+14428949 Msa1236010:CDS
TTGTTCCACCCCTACTATTT+TGG 0.218545 8_2:-14428713 None:intergenic
ATCCATCTATCTAGCCATTT+TGG 0.221443 8_2:-14428324 None:intergenic
ATCTAATTGTGCAAAGCTTA+TGG 0.234585 8_2:-14425467 None:intergenic
TGTGGATTATATTGATAATT+TGG 0.238180 8_2:-14428471 None:intergenic
AAGAAACACTTGTGGATCTT+TGG 0.252655 8_2:-14425895 None:intergenic
ACAACAAGTATGTCCTTAAA+TGG 0.267281 8_2:+14429468 Msa1236010:three_prime_UTR
AGTACAACAAGAAGAGTAAT+TGG 0.281665 8_2:-14429030 None:intergenic
CCAACTCCTCCTACATTATA+AGG 0.303150 8_2:-14428921 None:intergenic
GGAGTTGGTTATGGAAATTA+TGG 0.312677 8_2:+14428936 Msa1236010:CDS
TAGTGAGAGGTCACAATGTT+AGG 0.313052 8_2:+14427081 Msa1236010:CDS
TAGTGACACTGCTAGTGTTT+GGG 0.318138 8_2:+14427217 Msa1236010:CDS
AAGAGCACTTCGTGCGCTTA+AGG 0.319760 8_2:+14427037 Msa1236010:CDS
TGATTTATGATTCAATATAA+TGG 0.328529 8_2:-14425582 None:intergenic
CTCCATAGATCACACCAAAA+TGG 0.329388 8_2:+14428528 Msa1236010:CDS
GGTTGTGAACGGATCCATTT+TGG 0.332251 8_2:-14428542 None:intergenic
TACAAAACCATGGGAGAATA+GGG 0.336323 8_2:+14428350 Msa1236010:CDS
GAGAACCATGTATTGTGTTT+TGG 0.353766 8_2:+14429358 Msa1236010:three_prime_UTR
TGCAACGACTGCGGAGTATT+CGG 0.354857 8_2:+14429148 Msa1236010:three_prime_UTR
GAAGTAGTATTGCAGATGAT+TGG 0.362742 8_2:+14427501 Msa1236010:CDS
CTCATCTCAACCACCGTCTA+AGG 0.364361 8_2:-14429094 None:intergenic
TACACATTCAACAATGTTAC+AGG 0.365782 8_2:-14425845 None:intergenic
GGTTACAACTATGACACATT+AGG 0.366301 8_2:+14429169 Msa1236010:three_prime_UTR
CTACAAAACCATGGGAGAAT+AGG 0.367491 8_2:+14428349 Msa1236010:CDS
ACATCATGCTTCAACTTTGT+TGG 0.375678 8_2:-14426419 None:intergenic
TTTGCTCCTAGATATGAATA+AGG 0.377417 8_2:-14428432 None:intergenic
TCTCAACCTTATTCATATCT+AGG 0.385222 8_2:+14428426 Msa1236010:CDS
GGAACTTGTACCAAAATAGT+AGG 0.389102 8_2:+14428703 Msa1236010:CDS
TTAGTGACACTGCTAGTGTT+TGG 0.389305 8_2:+14427216 Msa1236010:CDS
TCGGTTGCTTGCATGTAATT+CGG 0.404052 8_2:-14428786 None:intergenic
TGTGAGTGAGTGAGAGGAGG+AGG 0.410940 8_2:-14425538 None:intergenic
AATGTAGGAGGAGTTGGTTA+TGG 0.413219 8_2:+14428927 Msa1236010:CDS
ATGCTACAAGTGTGGCTTCA+AGG 0.418682 8_2:+14426447 Msa1236010:CDS
GAGGTTTCACCTTCGTCAAC+CGG 0.431189 8_2:+14429068 Msa1236010:CDS
TTCTTTCCTTTGTTGCAACA+TGG 0.431704 8_2:-14427558 None:intergenic
ATTATTACATTGCAAACAAA+AGG 0.433968 8_2:-14429541 None:intergenic
CTTGACGACCGGACTTGAAT+AGG 0.434489 8_2:-14428600 None:intergenic
ATGTTCTGGCCTACCTCACT+TGG 0.436846 8_2:+14429425 Msa1236010:three_prime_UTR
CCTCTCAAACACCAAGCTTA+AGG 0.437895 8_2:+14428661 Msa1236010:CDS
GGTGTCGATGGCCTTTGCCT+TGG 0.439851 8_2:-14428843 None:intergenic
GTGAGGTAGGCCAGAACATA+TGG 0.440826 8_2:-14429421 None:intergenic
GACCAAAATGGCTAGATAGA+TGG 0.442192 8_2:+14428322 Msa1236010:CDS
TTCACTGTTTCATGATTCAC+TGG 0.442484 8_2:-14426377 None:intergenic
CAGAAAGGTTCAACTTGGTT+GGG 0.443221 8_2:-14425964 None:intergenic
CCTCCTACATTATAAGGATC+AGG 0.445401 8_2:-14428915 None:intergenic
CATATGGCCAGCATTTATCT+TGG 0.445786 8_2:-14429405 None:intergenic
AAGTAGTATTGCAGATGATT+GGG 0.451725 8_2:+14427502 Msa1236010:CDS
GGTACAAGTTCCCATTGTAA+TGG 0.452470 8_2:-14428692 None:intergenic
TGCCTTTGTGGTTGGTGATG+TGG 0.455595 8_2:-14428489 None:intergenic
AAACCTGTTGTGAAAATGCT+TGG 0.456509 8_2:-14427610 None:intergenic
CCTTATAATGTAGGAGGAGT+TGG 0.456681 8_2:+14428921 Msa1236010:CDS
TTCATTTATTTGCACAAATG+AGG 0.457015 8_2:+14425814 Msa1236010:five_prime_UTR
TAAATGCTGGCCATATGTTC+TGG 0.460943 8_2:+14429411 Msa1236010:three_prime_UTR
GGTCTAATTACCATTACAAT+GGG 0.460991 8_2:+14428682 Msa1236010:CDS
ACATTCACACTCTTGAAACT+TGG 0.462543 8_2:-14428975 None:intergenic
CTGCTAGTGTTTGGGATTCG+CGG 0.462678 8_2:+14427225 Msa1236010:CDS
AGAGCACTTCGTGCGCTTAA+GGG 0.465270 8_2:+14427038 Msa1236010:CDS
GAGTGAGTGAGAGGAGGAGG+GGG 0.466047 8_2:-14425535 None:intergenic
GGAAGAAAGGTAGTAGTTCA+TGG 0.469859 8_2:+14426082 Msa1236010:CDS
AATTCGAGCCTTTGCAGACT+CGG 0.472168 8_2:-14428805 None:intergenic
ACAGAAAGGTTCAACTTGGT+TGG 0.472834 8_2:-14425965 None:intergenic
TCAAGAAGAAGATGAAGAAA+AGG 0.473802 8_2:+14426185 Msa1236010:CDS
GTGAGTGAGTGAGAGGAGGA+GGG 0.475121 8_2:-14425537 None:intergenic
ACACACAGTTGAAGAAGTTA+AGG 0.475450 8_2:+14427535 Msa1236010:CDS
ATTTCACAGATTTGGAGAAA+TGG 0.475842 8_2:+14428258 Msa1236010:intron
AATGGTAGGACATCATCAAT+TGG 0.477140 8_2:+14428276 Msa1236010:CDS
GGAAAGAAGCAGCAATGAAG+AGG 0.480771 8_2:+14427573 Msa1236010:CDS
CGCAGAGTGCGCCAAGGCAA+AGG 0.484032 8_2:+14428832 Msa1236010:CDS
CACAACGAGGGCTTGACGAC+CGG 0.488125 8_2:-14428611 None:intergenic
TGTGGCTTCAAGGACAGATC+AGG 0.489395 8_2:+14426457 Msa1236010:CDS
ACATTATAAGGATCAGGAGC+AGG 0.491564 8_2:-14428909 None:intergenic
AATGTAGCAGCATCACATTA+TGG 0.491586 8_2:+14428993 Msa1236010:CDS
TGTTCTGGCCTACCTCACTT+GGG 0.492874 8_2:+14429426 Msa1236010:three_prime_UTR
CGTCAACCGGTGACCTTAGA+CGG 0.494779 8_2:+14429081 Msa1236010:CDS
CTATCCCTCTCTGGTGTCGA+TGG 0.494815 8_2:-14428855 None:intergenic
TGAGTGAGTGAGAGGAGGAG+GGG 0.494935 8_2:-14425536 None:intergenic
TAAAGTCTTTAAAGAAGAGA+AGG 0.495381 8_2:-14425938 None:intergenic
GAACTTGTACCAAAATAGTA+GGG 0.496333 8_2:+14428704 Msa1236010:CDS
GTGAAGTAGACCTTATCCTT+TGG 0.496611 8_2:-14427169 None:intergenic
GATCCGGATCTATCCCTCTC+TGG 0.498338 8_2:-14428864 None:intergenic
GATCCGGATCAGTAAAGAAA+AGG 0.499138 8_2:+14428877 Msa1236010:CDS
GCTCTTCCCCTATTCTCCCA+TGG 0.500010 8_2:-14428357 None:intergenic
ACATGCTCTTGCTGTGGCTA+TGG 0.501053 8_2:+14426487 Msa1236010:CDS
CTAAGAGTTCAAATCATGCT+AGG 0.504978 8_2:+14426567 Msa1236010:CDS
AGTCTGCAAAGGCTCGAATT+CGG 0.506521 8_2:+14428808 Msa1236010:CDS
TAAGGACATACTTGTTGTGT+AGG 0.509479 8_2:-14429463 None:intergenic
TTGCAGTGTTGAAGACAGAA+AGG 0.511229 8_2:-14425979 None:intergenic
AACTTGAAGAGAGACCAAAA+TGG 0.518980 8_2:+14428310 Msa1236010:CDS
TGCTGGAGACGGAGTAGTGA+TGG 0.522782 8_2:-14428568 None:intergenic
GCTCCTGATCCTTATAATGT+AGG 0.524729 8_2:+14428912 Msa1236010:CDS
CCTTAAGCTTGGTGTTTGAG+AGG 0.526162 8_2:-14428661 None:intergenic
GAAGCAATGGAATGCCTTTG+TGG 0.526434 8_2:-14428501 None:intergenic
AACCTGTTGTGAAAATGCTT+GGG 0.527631 8_2:-14427609 None:intergenic
AGGTCTAATTACCATTACAA+TGG 0.529005 8_2:+14428681 Msa1236010:CDS
GAGTTCTTGACCAAAGGATA+AGG 0.529710 8_2:+14427159 Msa1236010:CDS
ATCTTCAAGACATTTCAGAT+AGG 0.531548 8_2:+14427249 Msa1236010:CDS
AGCTACTGCTCAAGCTGCTG+TGG 0.535167 8_2:+14426529 Msa1236010:CDS
GCCTAAATAAAATAAGTGAA+TGG 0.536968 8_2:-14429202 None:intergenic
TCACAAAGAAACAAACAGTA+AGG 0.537387 8_2:+14425711 Msa1236010:five_prime_UTR
ATATAATGGTAGTCAGAGAC+AGG 0.538519 8_2:-14425568 None:intergenic
AAAGAAAAGAAGTTAGCATA+GGG 0.539161 8_2:+14429274 Msa1236010:three_prime_UTR
AGTGTCAGAAGGTATACCTT+AGG 0.542037 8_2:-14425412 None:intergenic
AAAAGAAAAGAAGTTAGCAT+AGG 0.544970 8_2:+14429273 Msa1236010:three_prime_UTR
GAGCACTTCGTGCGCTTAAG+GGG 0.545080 8_2:+14427039 Msa1236010:CDS
AGCAGCATCACATTATGGAT+TGG 0.546365 8_2:+14428998 Msa1236010:CDS
TCTTGTTGTACTGAGAGTCT+CGG 0.552055 8_2:+14429041 Msa1236010:CDS
GGAGCATTATGCTGCTGTTG+TGG 0.552104 8_2:+14426589 Msa1236010:CDS
AGCTTGGTGTTTGAGAGGTG+TGG 0.552600 8_2:-14428656 None:intergenic
TTAACTTCTTCAACTGTGTG+TGG 0.553478 8_2:-14427533 None:intergenic
GCAAGTACTATGTTGTTGCT+AGG 0.553496 8_2:+14429330 Msa1236010:three_prime_UTR
AACACTAGCAGTGTCACTAA+AGG 0.555204 8_2:-14427213 None:intergenic
AATCAAGACCTATTCAAGTC+CGG 0.556812 8_2:+14428592 Msa1236010:CDS
TACTGAGAGTCTCGGTGGCG+AGG 0.556933 8_2:+14429049 Msa1236010:CDS
ACACCAGAGAGGGATAGATC+CGG 0.558129 8_2:+14428861 Msa1236010:CDS
GAAAGAAGCAGCAATGAAGA+GGG 0.561558 8_2:+14427574 Msa1236010:CDS
AGTAGGGGTGGAACAAGTAA+TGG 0.561747 8_2:+14428720 Msa1236010:CDS
TTAAGGCCATGTTGCAACAA+AGG 0.561941 8_2:+14427552 Msa1236010:CDS
GGATGATCAAAGAAAGTGAA+AGG 0.565239 8_2:-14425373 None:intergenic
TGTGTTTGTGAGTGAGTGAG+AGG 0.565316 8_2:-14425544 None:intergenic
GAAGACAGAAAGGTTCAACT+TGG 0.566144 8_2:-14425969 None:intergenic
CACAGATTTGGAGAAATGGT+AGG 0.567014 8_2:+14428262 Msa1236010:intron
TCAACTACACTCAGTTTCAA+CGG 0.568119 8_2:+14429122 Msa1236010:three_prime_UTR
TAGGATGCCAAGATAAATGC+TGG 0.570025 8_2:+14429398 Msa1236010:three_prime_UTR
TCTATGGAGCGGTGAAGCAA+TGG 0.570311 8_2:-14428514 None:intergenic
TAAAACCAAAACACAATACA+TGG 0.570954 8_2:-14429363 None:intergenic
AGATTATGTTGAGTACTACA+AGG 0.572264 8_2:-14425626 None:intergenic
AAACAGACCCTAAAAGCCTA+AGG 0.572614 8_2:+14425396 Msa1236010:five_prime_UTR
ATTGATTGAAACAAAAGCAA+AGG 0.575368 8_2:-14429570 None:intergenic
CAATGGAATGCCTTTGTGGT+TGG 0.576689 8_2:-14428497 None:intergenic
GAGACGGAGTAGTGATGGAT+TGG 0.577607 8_2:-14428563 None:intergenic
TGGTAATTAGACCTTAAGCT+TGG 0.583935 8_2:-14428672 None:intergenic
CCTGATCCTTATAATGTAGG+AGG 0.585582 8_2:+14428915 Msa1236010:CDS
TGTGGCTATGGCTACAGCTG+AGG 0.587150 8_2:+14426499 Msa1236010:CDS
AAGCTGCTGTGGAAGTTGCA+AGG 0.590980 8_2:+14426540 Msa1236010:CDS
CAACCGGTGACCTTAGACGG+TGG 0.593652 8_2:+14429084 Msa1236010:CDS
TAAGAGTTCAAATCATGCTA+GGG 0.594458 8_2:+14426568 Msa1236010:CDS
AAGTTGCAAGCTTTAGTGAG+AGG 0.597297 8_2:+14427068 Msa1236010:CDS
TTGTACCAAAATAGTAGGGG+TGG 0.602042 8_2:+14428708 Msa1236010:CDS
AAGCAAGAGTTCTTGACCAA+AGG 0.602268 8_2:+14427153 Msa1236010:CDS
TTGGTGTTTGAGAGGTGTGG+TGG 0.605794 8_2:-14428653 None:intergenic
CAACCACCGTCTAAGGTCAC+CGG 0.606102 8_2:-14429087 None:intergenic
GTTTGTGAGTGAGTGAGAGG+AGG 0.612712 8_2:-14425541 None:intergenic
GTTCTGGCCTACCTCACTTG+GGG 0.613716 8_2:+14429427 Msa1236010:three_prime_UTR
GCAAGCAACCGAGTCTGCAA+AGG 0.614885 8_2:+14428797 Msa1236010:CDS
AAAGGTTCAACTTGGTTGGG+TGG 0.616143 8_2:-14425961 None:intergenic
TTGTTATGACAGTCAAGAGA+AGG 0.620921 8_2:+14427479 Msa1236010:intron
CATTATAAGGATCAGGAGCA+GGG 0.621699 8_2:-14428908 None:intergenic
ATGGATGGCTACAAAACCAT+GGG 0.623432 8_2:+14428341 Msa1236010:CDS
AGCAAGCAAAGATGACACTT+AGG 0.631862 8_2:+14427105 Msa1236010:CDS
GATGGATGGCTACAAAACCA+TGG 0.632158 8_2:+14428340 Msa1236010:CDS
ACAGCAGTGGGAGTGTCAGA+AGG 0.634678 8_2:-14425423 None:intergenic
AAAATGGCTAGATAGATGGA+TGG 0.637956 8_2:+14428326 Msa1236010:CDS
TTCGGTCGCAGAGTGCGCCA+AGG 0.638278 8_2:+14428826 Msa1236010:CDS
AAGAGACTCTATTAAGACTG+TGG 0.640275 8_2:+14428392 Msa1236010:CDS
CAACGGCTCTGCAACGACTG+CGG 0.642226 8_2:+14429139 Msa1236010:three_prime_UTR
AAGGCCATCGACACCAGAGA+GGG 0.647934 8_2:+14428851 Msa1236010:CDS
TTCAACACCCCAAGTGAGGT+AGG 0.648136 8_2:-14429434 None:intergenic
GCATGAAGGTGGACAGCAGT+GGG 0.649814 8_2:-14425435 None:intergenic
CTAAGGTCACCGGTTGACGA+AGG 0.652413 8_2:-14429077 None:intergenic
AGTGATGGATTGGTTGTGAA+CGG 0.660603 8_2:-14428553 None:intergenic
ATTGTTCAACACCCCAAGTG+AGG 0.663712 8_2:-14429438 None:intergenic
CTATCTTCTCGAACACAACG+AGG 0.664998 8_2:-14428624 None:intergenic
TATCTTCTCGAACACAACGA+GGG 0.667052 8_2:-14428623 None:intergenic
ACAAAACCATGGGAGAATAG+GGG 0.672684 8_2:+14428351 Msa1236010:CDS
ATCCACATCACCAACCACAA+AGG 0.673182 8_2:+14428487 Msa1236010:CDS
AACTTGTACCAAAATAGTAG+GGG 0.675624 8_2:+14428705 Msa1236010:CDS
TGAGAAACATGCTCTTGCTG+TGG 0.692861 8_2:+14426481 Msa1236010:CDS
TGTTGTACTGAGAGTCTCGG+TGG 0.693541 8_2:+14429044 Msa1236010:CDS
GGCATGAAGGTGGACAGCAG+TGG 0.698214 8_2:-14425436 None:intergenic
TGTTGTTAATGCTACAAGTG+TGG 0.698782 8_2:+14426439 Msa1236010:CDS
ATAAGGTCTACTTCACACGA+AGG 0.710315 8_2:+14427176 Msa1236010:CDS
AAAGGCCATCGACACCAGAG+AGG 0.713109 8_2:+14428850 Msa1236010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATGATTCAATATAA+TGG - chr8_2:14425585-14425604 None:intergenic 15.0%
!!! AATATTTTATAGTAGTACAT+TGG + chr8_2:14425668-14425687 Msa1236010:five_prime_UTR 15.0%
!! ATTAGAATGTAAAAACAAAA+GGG - chr8_2:14426261-14426280 None:intergenic 15.0%
!! AATTAGAATGTAAAAACAAA+AGG - chr8_2:14426262-14426281 None:intergenic 15.0%
!!! AATTTTGATTATAGTTATGT+TGG - chr8_2:14427315-14427334 None:intergenic 15.0%
!!! ATAGAACTTTTTAGTTATTT+TGG + chr8_2:14427417-14427436 Msa1236010:intron 15.0%
!!! AGAATTGATTTTGAATTTTT+TGG + chr8_2:14427851-14427870 Msa1236010:intron 15.0%
!!! ATATTTTGGAATTTCAATTA+TGG + chr8_2:14429515-14429534 Msa1236010:three_prime_UTR 15.0%
!!! AAATGTTGTTAAGTTTTACA+TGG + chr8_2:14426059-14426078 Msa1236010:exon 20.0%
!!! AATGTTGTTAAGTTTTACAT+GGG + chr8_2:14426060-14426079 Msa1236010:exon 20.0%
!!! TGTTTTTACATTCTAATTCA+TGG + chr8_2:14426264-14426283 Msa1236010:intron 20.0%
!!! AAAAGTACCTTTTTTTTTCT+TGG + chr8_2:14426991-14427010 Msa1236010:intron 20.0%
!! TTAGAAGCAAATATTATGTT+TGG + chr8_2:14427359-14427378 Msa1236010:intron 20.0%
!!! TGTGGATTATATTGATAATT+TGG - chr8_2:14428474-14428493 None:intergenic 20.0%
!!! ACCATTCACTTATTTTATTT+AGG + chr8_2:14429201-14429220 Msa1236010:three_prime_UTR 20.0%
!!! CACAATAACATGTAATATTT+TGG + chr8_2:14429501-14429520 Msa1236010:three_prime_UTR 20.0%
!! ATTATTACATTGCAAACAAA+AGG - chr8_2:14429544-14429563 None:intergenic 20.0%
! TTCATTTATTTGCACAAATG+AGG + chr8_2:14425814-14425833 Msa1236010:five_prime_UTR 25.0%
!! TAAAGTCTTTAAAGAAGAGA+AGG - chr8_2:14425941-14425960 None:intergenic 25.0%
!! ATGTTGTTAAGTTTTACATG+GGG + chr8_2:14426061-14426080 Msa1236010:exon 25.0%
!!! CTTTTTTCACTATCTTTTGT+TGG - chr8_2:14426138-14426157 None:intergenic 25.0%
! AAAGATAGTGAAAAAAGAAG+TGG + chr8_2:14426141-14426160 Msa1236010:CDS 25.0%
!!! AAAAAAGAAGGTGGATTTTT+AGG + chr8_2:14426351-14426370 Msa1236010:CDS 25.0%
!!! AACTTCTTTTAGAGGTTATT+TGG + chr8_2:14426616-14426635 Msa1236010:CDS 25.0%
! CTTTATGTTTGACAATATCA+TGG + chr8_2:14426743-14426762 Msa1236010:intron 25.0%
! AAACACACCAAGAAAAAAAA+AGG - chr8_2:14427001-14427020 None:intergenic 25.0%
!! AATATTTGCTTCTAAAAGCA+TGG - chr8_2:14427354-14427373 None:intergenic 25.0%
!!! TTGGAAACAAAAGTACTTTT+TGG + chr8_2:14427378-14427397 Msa1236010:intron 25.0%
!!! TATTTTGGTGTTCTGTTTAT+AGG + chr8_2:14427432-14427451 Msa1236010:intron 25.0%
!! TTATGTGTTTGAATCTTTTG+TGG + chr8_2:14427456-14427475 Msa1236010:intron 25.0%
! AGAAAATAAAGCGAAAAAGA+GGG - chr8_2:14427644-14427663 None:intergenic 25.0%
! AAGAAAATAAAGCGAAAAAG+AGG - chr8_2:14427645-14427664 None:intergenic 25.0%
!! ATTTTTCCTCATAGTGATTT+TGG - chr8_2:14427703-14427722 None:intergenic 25.0%
!! AGGAAAAATCTACATTTTGA+AGG + chr8_2:14427714-14427733 Msa1236010:intron 25.0%
! TCTAACTTGAAACTAGAATA+TGG + chr8_2:14428035-14428054 Msa1236010:intron 25.0%
!!! ATATTCAACTCACTTTTACA+TGG + chr8_2:14428095-14428114 Msa1236010:intron 25.0%
! ATTAGCTAAGAAAATTGCTT+TGG - chr8_2:14428227-14428246 None:intergenic 25.0%
!! AAGCAATTTTCTTAGCTAAT+TGG + chr8_2:14428227-14428246 Msa1236010:intron 25.0%
! ATTTGACAATTTCACAGATT+TGG + chr8_2:14428250-14428269 Msa1236010:intron 25.0%
! GAAATTATGGACATAGTTTA+AGG + chr8_2:14428949-14428968 Msa1236010:CDS 25.0%
! GCCTAAATAAAATAAGTGAA+TGG - chr8_2:14429205-14429224 None:intergenic 25.0%
!! ATGCTAACTTCTTTTCTTTT+GGG - chr8_2:14429273-14429292 None:intergenic 25.0%
!! TATGCTAACTTCTTTTCTTT+TGG - chr8_2:14429274-14429293 None:intergenic 25.0%
! AAAAGAAAAGAAGTTAGCAT+AGG + chr8_2:14429273-14429292 Msa1236010:three_prime_UTR 25.0%
! AAAGAAAAGAAGTTAGCATA+GGG + chr8_2:14429274-14429293 Msa1236010:three_prime_UTR 25.0%
! TAAAACCAAAACACAATACA+TGG - chr8_2:14429366-14429385 None:intergenic 25.0%
!!! GGTTTTATCATTTGTTTGTT+AGG + chr8_2:14429379-14429398 Msa1236010:three_prime_UTR 25.0%
! ATTGATTGAAACAAAAGCAA+AGG - chr8_2:14429573-14429592 None:intergenic 25.0%
ATCTAATTGTGCAAAGCTTA+TGG - chr8_2:14425470-14425489 None:intergenic 30.0%
AGATTATGTTGAGTACTACA+AGG - chr8_2:14425629-14425648 None:intergenic 30.0%
TCACAAAGAAACAAACAGTA+AGG + chr8_2:14425711-14425730 Msa1236010:five_prime_UTR 30.0%
TACACATTCAACAATGTTAC+AGG - chr8_2:14425848-14425867 None:intergenic 30.0%
AAAGCAAAAAGAAACACTTG+TGG - chr8_2:14425906-14425925 None:intergenic 30.0%
! TCAACACTGCAAAGTTTTTA+AGG + chr8_2:14425990-14426009 Msa1236010:five_prime_UTR 30.0%
TCAAGAAGAAGATGAAGAAA+AGG + chr8_2:14426185-14426204 Msa1236010:CDS 30.0%
TGTAGAAGAGAGAAAAAAGA+AGG + chr8_2:14426339-14426358 Msa1236010:intron 30.0%
TAAGAGTTCAAATCATGCTA+GGG + chr8_2:14426568-14426587 Msa1236010:CDS 30.0%
ATCTTCAAGACATTTCAGAT+AGG + chr8_2:14427249-14427268 Msa1236010:CDS 30.0%
! TAGTAAGTCATATGTTGCTT+TGG + chr8_2:14427279-14427298 Msa1236010:intron 30.0%
!!! CTTTTTGGTTAGAAAAGCAA+AGG + chr8_2:14427393-14427412 Msa1236010:intron 30.0%
AAGTAGTATTGCAGATGATT+GGG + chr8_2:14427502-14427521 Msa1236010:CDS 30.0%
! TTGTTCTAAGTCTGCATATT+TGG + chr8_2:14427663-14427682 Msa1236010:intron 30.0%
!!! GAATTTTTTGGATCTTCTCA+AGG + chr8_2:14427863-14427882 Msa1236010:intron 30.0%
TTCAATGTAATCACTAGTGT+CGG - chr8_2:14427925-14427944 None:intergenic 30.0%
! TTTGCCTCAACAATGATTTT+TGG + chr8_2:14428062-14428081 Msa1236010:intron 30.0%
GATACCAAAAATCATTGTTG+AGG - chr8_2:14428069-14428088 None:intergenic 30.0%
!! AAGAAAATTGCTTTGGTGTT+TGG - chr8_2:14428220-14428239 None:intergenic 30.0%
ATTTCACAGATTTGGAGAAA+TGG + chr8_2:14428258-14428277 Msa1236010:intron 30.0%
TCTCAACCTTATTCATATCT+AGG + chr8_2:14428426-14428445 Msa1236010:CDS 30.0%
TTTGCTCCTAGATATGAATA+AGG - chr8_2:14428435-14428454 None:intergenic 30.0%
AGGTCTAATTACCATTACAA+TGG + chr8_2:14428681-14428700 Msa1236010:CDS 30.0%
GGTCTAATTACCATTACAAT+GGG + chr8_2:14428682-14428701 Msa1236010:CDS 30.0%
GAACTTGTACCAAAATAGTA+GGG + chr8_2:14428704-14428723 Msa1236010:CDS 30.0%
AACTTGTACCAAAATAGTAG+GGG + chr8_2:14428705-14428724 Msa1236010:CDS 30.0%
AGTACAACAAGAAGAGTAAT+TGG - chr8_2:14429033-14429052 None:intergenic 30.0%
! TGCTAACTTCTTTTCTTTTG+GGG - chr8_2:14429272-14429291 None:intergenic 30.0%
GAACCTAAAAATCTGATGTT+AGG + chr8_2:14429298-14429317 Msa1236010:three_prime_UTR 30.0%
! ACTCCTAACATCAGATTTTT+AGG - chr8_2:14429304-14429323 None:intergenic 30.0%
ACAACAAGTATGTCCTTAAA+TGG + chr8_2:14429468-14429487 Msa1236010:three_prime_UTR 30.0%
! AGGTATACCTTAGGCTTTTA+GGG - chr8_2:14425406-14425425 None:intergenic 35.0%
! AAGGTATACCTTAGGCTTTT+AGG - chr8_2:14425407-14425426 None:intergenic 35.0%
! TATGGATTTTTGTGGCATGA+AGG - chr8_2:14425452-14425471 None:intergenic 35.0%
! GCAAAGCTTATGGATTTTTG+TGG - chr8_2:14425460-14425479 None:intergenic 35.0%
ATATAATGGTAGTCAGAGAC+AGG - chr8_2:14425571-14425590 None:intergenic 35.0%
AAGAAACACTTGTGGATCTT+TGG - chr8_2:14425898-14425917 None:intergenic 35.0%
AGAAGAGAAGGAGAAAAACA+AGG - chr8_2:14425929-14425948 None:intergenic 35.0%
! AAGTTTTACATGGGGAAGAA+AGG + chr8_2:14426069-14426088 Msa1236010:exon 35.0%
AGAAGAGAGAAAAAAGAAGG+TGG + chr8_2:14426342-14426361 Msa1236010:intron 35.0%
TTCACTGTTTCATGATTCAC+TGG - chr8_2:14426380-14426399 None:intergenic 35.0%
ACATCATGCTTCAACTTTGT+TGG - chr8_2:14426422-14426441 None:intergenic 35.0%
TGTTGTTAATGCTACAAGTG+TGG + chr8_2:14426439-14426458 Msa1236010:CDS 35.0%
CTAAGAGTTCAAATCATGCT+AGG + chr8_2:14426567-14426586 Msa1236010:CDS 35.0%
! GTGGTTCAAACTTCTTTTAG+AGG + chr8_2:14426608-14426627 Msa1236010:CDS 35.0%
AACAGATCTAATTGCCTGTT+TGG + chr8_2:14426650-14426669 Msa1236010:intron 35.0%
AACTTGCAGTAATTCCAAAC+AGG - chr8_2:14426667-14426686 None:intergenic 35.0%
! GTGTAGTTTTTGCCAAATCA+CGG - chr8_2:14426890-14426909 None:intergenic 35.0%
TGTGATTTCGTCAAATTCAC+TGG + chr8_2:14426940-14426959 Msa1236010:intron 35.0%
GTGATTTCGTCAAATTCACT+GGG + chr8_2:14426941-14426960 Msa1236010:intron 35.0%
TTGTTATGACAGTCAAGAGA+AGG + chr8_2:14427479-14427498 Msa1236010:intron 35.0%
GAAGTAGTATTGCAGATGAT+TGG + chr8_2:14427501-14427520 Msa1236010:CDS 35.0%
TTAACTTCTTCAACTGTGTG+TGG - chr8_2:14427536-14427555 None:intergenic 35.0%
ACACACAGTTGAAGAAGTTA+AGG + chr8_2:14427535-14427554 Msa1236010:CDS 35.0%
TTCTTTCCTTTGTTGCAACA+TGG - chr8_2:14427561-14427580 None:intergenic 35.0%
AACCTGTTGTGAAAATGCTT+GGG - chr8_2:14427612-14427631 None:intergenic 35.0%
AAACCTGTTGTGAAAATGCT+TGG - chr8_2:14427613-14427632 None:intergenic 35.0%
AGTTTGCCAAAATCACTATG+AGG + chr8_2:14427694-14427713 Msa1236010:intron 35.0%
! TGTATGTGTGTTTCCACTTT+TGG + chr8_2:14427802-14427821 Msa1236010:intron 35.0%
TCAAGGAGAATTGAGTTTGA+AGG + chr8_2:14427880-14427899 Msa1236010:intron 35.0%
AAGTTAGAACCTATGAAGCA+CGG - chr8_2:14428023-14428042 None:intergenic 35.0%
ATGAATTGACTGTGTAACAC+TGG - chr8_2:14428170-14428189 None:intergenic 35.0%
AATGGTAGGACATCATCAAT+TGG + chr8_2:14428276-14428295 Msa1236010:CDS 35.0%
AACTTGAAGAGAGACCAAAA+TGG + chr8_2:14428310-14428329 Msa1236010:CDS 35.0%
! ATCCATCTATCTAGCCATTT+TGG - chr8_2:14428327-14428346 None:intergenic 35.0%
AAAATGGCTAGATAGATGGA+TGG + chr8_2:14428326-14428345 Msa1236010:CDS 35.0%
TACAAAACCATGGGAGAATA+GGG + chr8_2:14428350-14428369 Msa1236010:CDS 35.0%
AAGAGACTCTATTAAGACTG+TGG + chr8_2:14428392-14428411 Msa1236010:CDS 35.0%
!! ATCCATTTTGGTGTGATCTA+TGG - chr8_2:14428533-14428552 None:intergenic 35.0%
! TGAATAGGTCTTGATTTTGC+TGG - chr8_2:14428588-14428607 None:intergenic 35.0%
AATCAAGACCTATTCAAGTC+CGG + chr8_2:14428592-14428611 Msa1236010:CDS 35.0%
TGGTAATTAGACCTTAAGCT+TGG - chr8_2:14428675-14428694 None:intergenic 35.0%
GGAACTTGTACCAAAATAGT+AGG + chr8_2:14428703-14428722 Msa1236010:CDS 35.0%
! AAGCCTTTTCTTTACTGATC+CGG - chr8_2:14428883-14428902 None:intergenic 35.0%
! GGAGTTGGTTATGGAAATTA+TGG + chr8_2:14428936-14428955 Msa1236010:CDS 35.0%
ACATTCACACTCTTGAAACT+TGG - chr8_2:14428978-14428997 None:intergenic 35.0%
AATGTAGCAGCATCACATTA+TGG + chr8_2:14428993-14429012 Msa1236010:CDS 35.0%
TCAACTACACTCAGTTTCAA+CGG + chr8_2:14429122-14429141 Msa1236010:three_prime_UTR 35.0%
GGTTACAACTATGACACATT+AGG + chr8_2:14429169-14429188 Msa1236010:three_prime_UTR 35.0%
!!! CTTTTCTTTTGGGGTGAAAA+GGG - chr8_2:14429263-14429282 None:intergenic 35.0%
!!! TCTTTTCTTTTGGGGTGAAA+AGG - chr8_2:14429264-14429283 None:intergenic 35.0%
! GAGAACCATGTATTGTGTTT+TGG + chr8_2:14429358-14429377 Msa1236010:three_prime_UTR 35.0%
!! TAAGGACATACTTGTTGTGT+AGG - chr8_2:14429466-14429485 None:intergenic 35.0%
!!! TTAGGCTTTTAGGGTCTGTT+TGG - chr8_2:14425397-14425416 None:intergenic 40.0%
AAACAGACCCTAAAAGCCTA+AGG + chr8_2:14425396-14425415 Msa1236010:five_prime_UTR 40.0%
! AGTGTCAGAAGGTATACCTT+AGG - chr8_2:14425415-14425434 None:intergenic 40.0%
CAGAAAGGTTCAACTTGGTT+GGG - chr8_2:14425967-14425986 None:intergenic 40.0%
ACAGAAAGGTTCAACTTGGT+TGG - chr8_2:14425968-14425987 None:intergenic 40.0%
GAAGACAGAAAGGTTCAACT+TGG - chr8_2:14425972-14425991 None:intergenic 40.0%
! TTGCAGTGTTGAAGACAGAA+AGG - chr8_2:14425982-14426001 None:intergenic 40.0%
GGAAGAAAGGTAGTAGTTCA+TGG + chr8_2:14426082-14426101 Msa1236010:CDS 40.0%
TTACAGTGACACCGTGATTT+CGG - chr8_2:14426692-14426711 None:intergenic 40.0%
TCCAAAATCATTGTGGCCAT+TGG - chr8_2:14426833-14426852 None:intergenic 40.0%
! TGGCCACAATGATTTTGGAT+CGG + chr8_2:14426834-14426853 Msa1236010:intron 40.0%
! TTTTGGATCGGTGGTGAATT+TGG + chr8_2:14426846-14426865 Msa1236010:intron 40.0%
CAAATCACGGTTGAACATTG+TGG - chr8_2:14426877-14426896 None:intergenic 40.0%
CACAATGTTCAACCGTGATT+TGG + chr8_2:14426875-14426894 Msa1236010:intron 40.0%
TTGACGAAATCACAGTGACA+CGG - chr8_2:14426934-14426953 None:intergenic 40.0%
TTGGTGTGTTTGAACCTTGA+AGG + chr8_2:14427010-14427029 Msa1236010:intron 40.0%
! AAGTTGCAAGCTTTAGTGAG+AGG + chr8_2:14427068-14427087 Msa1236010:CDS 40.0%
TAGTGAGAGGTCACAATGTT+AGG + chr8_2:14427081-14427100 Msa1236010:CDS 40.0%
AGCAAGCAAAGATGACACTT+AGG + chr8_2:14427105-14427124 Msa1236010:CDS 40.0%
AAGCAAGAGTTCTTGACCAA+AGG + chr8_2:14427153-14427172 Msa1236010:CDS 40.0%
GAGTTCTTGACCAAAGGATA+AGG + chr8_2:14427159-14427178 Msa1236010:CDS 40.0%
GTGAAGTAGACCTTATCCTT+TGG - chr8_2:14427172-14427191 None:intergenic 40.0%
ATAAGGTCTACTTCACACGA+AGG + chr8_2:14427176-14427195 Msa1236010:CDS 40.0%
! AACACTAGCAGTGTCACTAA+AGG - chr8_2:14427216-14427235 None:intergenic 40.0%
TTAGTGACACTGCTAGTGTT+TGG + chr8_2:14427216-14427235 Msa1236010:CDS 40.0%
TAGTGACACTGCTAGTGTTT+GGG + chr8_2:14427217-14427236 Msa1236010:CDS 40.0%
TTAAGGCCATGTTGCAACAA+AGG + chr8_2:14427552-14427571 Msa1236010:CDS 40.0%
GAAAGAAGCAGCAATGAAGA+GGG + chr8_2:14427574-14427593 Msa1236010:CDS 40.0%
!! ATGTGTGTTTCCACTTTTGG+AGG + chr8_2:14427805-14427824 Msa1236010:intron 40.0%
CAATTTCACTCCTCCAAAAG+TGG - chr8_2:14427818-14427837 None:intergenic 40.0%
CACAGATTTGGAGAAATGGT+AGG + chr8_2:14428262-14428281 Msa1236010:intron 40.0%
GACCAAAATGGCTAGATAGA+TGG + chr8_2:14428322-14428341 Msa1236010:CDS 40.0%
ATGGATGGCTACAAAACCAT+GGG + chr8_2:14428341-14428360 Msa1236010:CDS 40.0%
CTACAAAACCATGGGAGAAT+AGG + chr8_2:14428349-14428368 Msa1236010:CDS 40.0%
ACAAAACCATGGGAGAATAG+GGG + chr8_2:14428351-14428370 Msa1236010:CDS 40.0%
!! TTTTGGTGTGATCTATGGAG+CGG - chr8_2:14428528-14428547 None:intergenic 40.0%
CTCCATAGATCACACCAAAA+TGG + chr8_2:14428528-14428547 Msa1236010:CDS 40.0%
!! AGTGATGGATTGGTTGTGAA+CGG - chr8_2:14428556-14428575 None:intergenic 40.0%
TATCTTCTCGAACACAACGA+GGG - chr8_2:14428626-14428645 None:intergenic 40.0%
GGTACAAGTTCCCATTGTAA+TGG - chr8_2:14428695-14428714 None:intergenic 40.0%
TTGTACCAAAATAGTAGGGG+TGG + chr8_2:14428708-14428727 Msa1236010:CDS 40.0%
! TTGTTCCACCCCTACTATTT+TGG - chr8_2:14428716-14428735 None:intergenic 40.0%
TCGGTTGCTTGCATGTAATT+CGG - chr8_2:14428789-14428808 None:intergenic 40.0%
GATCCGGATCAGTAAAGAAA+AGG + chr8_2:14428877-14428896 Msa1236010:CDS 40.0%
CATTATAAGGATCAGGAGCA+GGG - chr8_2:14428911-14428930 None:intergenic 40.0%
ACATTATAAGGATCAGGAGC+AGG - chr8_2:14428912-14428931 None:intergenic 40.0%
GCTCCTGATCCTTATAATGT+AGG + chr8_2:14428912-14428931 Msa1236010:CDS 40.0%
CCTCCTACATTATAAGGATC+AGG - chr8_2:14428918-14428937 None:intergenic 40.0%
CCTGATCCTTATAATGTAGG+AGG + chr8_2:14428915-14428934 Msa1236010:CDS 40.0%
CCAACTCCTCCTACATTATA+AGG - chr8_2:14428924-14428943 None:intergenic 40.0%
CCTTATAATGTAGGAGGAGT+TGG + chr8_2:14428921-14428940 Msa1236010:CDS 40.0%
! AATGTAGGAGGAGTTGGTTA+TGG + chr8_2:14428927-14428946 Msa1236010:CDS 40.0%
AGCAGCATCACATTATGGAT+TGG + chr8_2:14428998-14429017 Msa1236010:CDS 40.0%
! TCTTGTTGTACTGAGAGTCT+CGG + chr8_2:14429041-14429060 Msa1236010:CDS 40.0%
GCAAGTACTATGTTGTTGCT+AGG + chr8_2:14429330-14429349 Msa1236010:three_prime_UTR 40.0%
TAGGATGCCAAGATAAATGC+TGG + chr8_2:14429398-14429417 Msa1236010:three_prime_UTR 40.0%
CATATGGCCAGCATTTATCT+TGG - chr8_2:14429408-14429427 None:intergenic 40.0%
TAAATGCTGGCCATATGTTC+TGG + chr8_2:14429411-14429430 Msa1236010:three_prime_UTR 40.0%
GTGACAAGACAGTCCATTTA+AGG - chr8_2:14429484-14429503 None:intergenic 40.0%
! GGATTTTTGTGGCATGAAGG+TGG - chr8_2:14425449-14425468 None:intergenic 45.0%
TGTGTTTGTGAGTGAGTGAG+AGG - chr8_2:14425547-14425566 None:intergenic 45.0%
AAAGGTTCAACTTGGTTGGG+TGG - chr8_2:14425964-14425983 None:intergenic 45.0%
ATGCTACAAGTGTGGCTTCA+AGG + chr8_2:14426447-14426466 Msa1236010:CDS 45.0%
TGAGAAACATGCTCTTGCTG+TGG + chr8_2:14426481-14426500 Msa1236010:CDS 45.0%
CTGCAAGTTTGCCGAAATCA+CGG + chr8_2:14426678-14426697 Msa1236010:intron 45.0%
ACTCATTGTGATCCTGCCAA+TGG + chr8_2:14426814-14426833 Msa1236010:intron 45.0%
AAATCATTGTGGCCATTGGC+AGG - chr8_2:14426829-14426848 None:intergenic 45.0%
! GCCAATGGCCACAATGATTT+TGG + chr8_2:14426829-14426848 Msa1236010:intron 45.0%
CCACCGATCCAAAATCATTG+TGG - chr8_2:14426840-14426859 None:intergenic 45.0%
! CCACAATGATTTTGGATCGG+TGG + chr8_2:14426837-14426856 Msa1236010:intron 45.0%
AAGTGCTCTTCTTGCCTTCA+AGG - chr8_2:14427027-14427046 None:intergenic 45.0%
GGAAAGAAGCAGCAATGAAG+AGG + chr8_2:14427573-14427592 Msa1236010:CDS 45.0%
! GTCCCAAGCATTTTCACAAC+AGG + chr8_2:14427607-14427626 Msa1236010:CDS 45.0%
CGATGAGTTCGACAAAGTCT+AGG - chr8_2:14427758-14427777 None:intergenic 45.0%
!! GTAATCACTAGTGTCGGTGT+CGG - chr8_2:14427919-14427938 None:intergenic 45.0%
TATCTGTGTCCGTGCTTCAT+AGG + chr8_2:14428011-14428030 Msa1236010:intron 45.0%
GATGGATGGCTACAAAACCA+TGG + chr8_2:14428340-14428359 Msa1236010:CDS 45.0%
ATCCACATCACCAACCACAA+AGG + chr8_2:14428487-14428506 Msa1236010:CDS 45.0%
CAATGGAATGCCTTTGTGGT+TGG - chr8_2:14428500-14428519 None:intergenic 45.0%
GAAGCAATGGAATGCCTTTG+TGG - chr8_2:14428504-14428523 None:intergenic 45.0%
! GGTTGTGAACGGATCCATTT+TGG - chr8_2:14428545-14428564 None:intergenic 45.0%
! GGTCTTGATTTTGCTGGAGA+CGG - chr8_2:14428582-14428601 None:intergenic 45.0%
CTATCTTCTCGAACACAACG+AGG - chr8_2:14428627-14428646 None:intergenic 45.0%
!! CCTTAAGCTTGGTGTTTGAG+AGG - chr8_2:14428664-14428683 None:intergenic 45.0%
CCTCTCAAACACCAAGCTTA+AGG + chr8_2:14428661-14428680 Msa1236010:CDS 45.0%
AGTAGGGGTGGAACAAGTAA+TGG + chr8_2:14428720-14428739 Msa1236010:CDS 45.0%
AATTCGAGCCTTTGCAGACT+CGG - chr8_2:14428808-14428827 None:intergenic 45.0%
AGTCTGCAAAGGCTCGAATT+CGG + chr8_2:14428808-14428827 Msa1236010:CDS 45.0%
ATTGTTCAACACCCCAAGTG+AGG - chr8_2:14429441-14429460 None:intergenic 45.0%
!!! TTTACATAATAATAAAATTT+TGG + chr8_2:14425493-14425512 Msa1236010:five_prime_UTR 5.0%
!!! ATTATATATTCATTTTTTTT+TGG + chr8_2:14425744-14425763 Msa1236010:five_prime_UTR 5.0%
GTTTGTGAGTGAGTGAGAGG+AGG - chr8_2:14425544-14425563 None:intergenic 50.0%
TGTGGCTTCAAGGACAGATC+AGG + chr8_2:14426457-14426476 Msa1236010:CDS 50.0%
! ACATGCTCTTGCTGTGGCTA+TGG + chr8_2:14426487-14426506 Msa1236010:CDS 50.0%
AAGCTGCTGTGGAAGTTGCA+AGG + chr8_2:14426540-14426559 Msa1236010:CDS 50.0%
! GGAGCATTATGCTGCTGTTG+TGG + chr8_2:14426589-14426608 Msa1236010:CDS 50.0%
!! AAGAGCACTTCGTGCGCTTA+AGG + chr8_2:14427037-14427056 Msa1236010:CDS 50.0%
! AGAGCACTTCGTGCGCTTAA+GGG + chr8_2:14427038-14427057 Msa1236010:CDS 50.0%
! CTGCTAGTGTTTGGGATTCG+CGG + chr8_2:14427225-14427244 Msa1236010:CDS 50.0%
! TTGAGTTTGAAGGCGTGTCC+CGG + chr8_2:14427890-14427909 Msa1236010:intron 50.0%
CTGACACAAACACGTCGACA+CGG - chr8_2:14427973-14427992 None:intergenic 50.0%
GTTTGTGTCAGTGTCGTGTC+CGG + chr8_2:14427982-14428001 Msa1236010:intron 50.0%
ACGGACACAGATACGTACAC+CGG - chr8_2:14428004-14428023 None:intergenic 50.0%
!! TGCCTTTGTGGTTGGTGATG+TGG - chr8_2:14428492-14428511 None:intergenic 50.0%
TCTATGGAGCGGTGAAGCAA+TGG - chr8_2:14428517-14428536 None:intergenic 50.0%
!! GAGACGGAGTAGTGATGGAT+TGG - chr8_2:14428566-14428585 None:intergenic 50.0%
CTTGACGACCGGACTTGAAT+AGG - chr8_2:14428603-14428622 None:intergenic 50.0%
! TTGGTGTTTGAGAGGTGTGG+TGG - chr8_2:14428656-14428675 None:intergenic 50.0%
!! AGCTTGGTGTTTGAGAGGTG+TGG - chr8_2:14428659-14428678 None:intergenic 50.0%
ACACCAGAGAGGGATAGATC+CGG + chr8_2:14428861-14428880 Msa1236010:CDS 50.0%
TGTTGTACTGAGAGTCTCGG+TGG + chr8_2:14429044-14429063 Msa1236010:CDS 50.0%
GAGGTTTCACCTTCGTCAAC+CGG + chr8_2:14429068-14429087 Msa1236010:CDS 50.0%
CTCATCTCAACCACCGTCTA+AGG - chr8_2:14429097-14429116 None:intergenic 50.0%
! TGCAACGACTGCGGAGTATT+CGG + chr8_2:14429148-14429167 Msa1236010:three_prime_UTR 50.0%
GTGAGGTAGGCCAGAACATA+TGG - chr8_2:14429424-14429443 None:intergenic 50.0%
ATGTTCTGGCCTACCTCACT+TGG + chr8_2:14429425-14429444 Msa1236010:three_prime_UTR 50.0%
TGTTCTGGCCTACCTCACTT+GGG + chr8_2:14429426-14429445 Msa1236010:three_prime_UTR 50.0%
TTCAACACCCCAAGTGAGGT+AGG - chr8_2:14429437-14429456 None:intergenic 50.0%
!! ACAGCAGTGGGAGTGTCAGA+AGG - chr8_2:14425426-14425445 None:intergenic 55.0%
GCATGAAGGTGGACAGCAGT+GGG - chr8_2:14425438-14425457 None:intergenic 55.0%
TGAGTGAGTGAGAGGAGGAG+GGG - chr8_2:14425539-14425558 None:intergenic 55.0%
GTGAGTGAGTGAGAGGAGGA+GGG - chr8_2:14425540-14425559 None:intergenic 55.0%
TGTGAGTGAGTGAGAGGAGG+AGG - chr8_2:14425541-14425560 None:intergenic 55.0%
TGTGGCTATGGCTACAGCTG+AGG + chr8_2:14426499-14426518 Msa1236010:CDS 55.0%
AGCTACTGCTCAAGCTGCTG+TGG + chr8_2:14426529-14426548 Msa1236010:CDS 55.0%
GAGCACTTCGTGCGCTTAAG+GGG + chr8_2:14427039-14427058 Msa1236010:CDS 55.0%
GCTCTTCCCCTATTCTCCCA+TGG - chr8_2:14428360-14428379 None:intergenic 55.0%
TGCTGGAGACGGAGTAGTGA+TGG - chr8_2:14428571-14428590 None:intergenic 55.0%
GCAAGCAACCGAGTCTGCAA+AGG + chr8_2:14428797-14428816 Msa1236010:CDS 55.0%
AAAGGCCATCGACACCAGAG+AGG + chr8_2:14428850-14428869 Msa1236010:CDS 55.0%
AAGGCCATCGACACCAGAGA+GGG + chr8_2:14428851-14428870 Msa1236010:CDS 55.0%
! CTATCCCTCTCTGGTGTCGA+TGG - chr8_2:14428858-14428877 None:intergenic 55.0%
GATCCGGATCTATCCCTCTC+TGG - chr8_2:14428867-14428886 None:intergenic 55.0%
! CTAAGGTCACCGGTTGACGA+AGG - chr8_2:14429080-14429099 None:intergenic 55.0%
CGTCAACCGGTGACCTTAGA+CGG + chr8_2:14429081-14429100 Msa1236010:CDS 55.0%
! CAACCACCGTCTAAGGTCAC+CGG - chr8_2:14429090-14429109 None:intergenic 55.0%
GTTCTGGCCTACCTCACTTG+GGG + chr8_2:14429427-14429446 Msa1236010:three_prime_UTR 55.0%
GGCATGAAGGTGGACAGCAG+TGG - chr8_2:14425439-14425458 None:intergenic 60.0%
GAGTGAGTGAGAGGAGGAGG+GGG - chr8_2:14425538-14425557 None:intergenic 60.0%
!! TAGTGTCGGTGTCGGACACC+GGG - chr8_2:14427911-14427930 None:intergenic 60.0%
!! CTAGTGTCGGTGTCGGACAC+CGG - chr8_2:14427912-14427931 None:intergenic 60.0%
CACAACGAGGGCTTGACGAC+CGG - chr8_2:14428614-14428633 None:intergenic 60.0%
!! GGTGTCGATGGCCTTTGCCT+TGG - chr8_2:14428846-14428865 None:intergenic 60.0%
TACTGAGAGTCTCGGTGGCG+AGG + chr8_2:14429049-14429068 Msa1236010:CDS 60.0%
CAACCGGTGACCTTAGACGG+TGG + chr8_2:14429084-14429103 Msa1236010:CDS 60.0%
CAACGGCTCTGCAACGACTG+CGG + chr8_2:14429139-14429158 Msa1236010:three_prime_UTR 60.0%
TTCGGTCGCAGAGTGCGCCA+AGG + chr8_2:14428826-14428845 Msa1236010:CDS 65.0%
CGCAGAGTGCGCCAAGGCAA+AGG + chr8_2:14428832-14428851 Msa1236010:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 14425376 14429606 14425376 ID=Msa1236010;Name=Msa1236010
chr8_2 mRNA 14425376 14429606 14425376 ID=Msa1236010-mRNA-1;Parent=Msa1236010;Name=Msa1236010-mRNA-1;_AED=0.05;_eAED=0.05;_QI=702|1|1|1|1|1|5|491|552
chr8_2 exon 14425376 14426206 14425376 ID=Msa1236010-mRNA-1:exon:5498;Parent=Msa1236010-mRNA-1
chr8_2 exon 14426344 14426637 14426344 ID=Msa1236010-mRNA-1:exon:5499;Parent=Msa1236010-mRNA-1
chr8_2 exon 14427032 14427280 14427032 ID=Msa1236010-mRNA-1:exon:5500;Parent=Msa1236010-mRNA-1
chr8_2 exon 14427491 14427628 14427491 ID=Msa1236010-mRNA-1:exon:5501;Parent=Msa1236010-mRNA-1
chr8_2 exon 14428267 14429606 14428267 ID=Msa1236010-mRNA-1:exon:5502;Parent=Msa1236010-mRNA-1
chr8_2 five_prime_UTR 14425376 14426077 14425376 ID=Msa1236010-mRNA-1:five_prime_utr;Parent=Msa1236010-mRNA-1
chr8_2 CDS 14426078 14426206 14426078 ID=Msa1236010-mRNA-1:cds;Parent=Msa1236010-mRNA-1
chr8_2 CDS 14426344 14426637 14426344 ID=Msa1236010-mRNA-1:cds;Parent=Msa1236010-mRNA-1
chr8_2 CDS 14427032 14427280 14427032 ID=Msa1236010-mRNA-1:cds;Parent=Msa1236010-mRNA-1
chr8_2 CDS 14427491 14427628 14427491 ID=Msa1236010-mRNA-1:cds;Parent=Msa1236010-mRNA-1
chr8_2 CDS 14428267 14429115 14428267 ID=Msa1236010-mRNA-1:cds;Parent=Msa1236010-mRNA-1
chr8_2 three_prime_UTR 14429116 14429606 14429116 ID=Msa1236010-mRNA-1:three_prime_utr;Parent=Msa1236010-mRNA-1
Gene Sequence

>Msa1236010

ATGGGGAAGAAAGGTAGTAGTTCATGGTTAACTGCTGTGAAAAGAGCATTCAGATCTCCAACAAAAGATAGTGAAAAAAGAAGTGGTAGAAGAAGAGAAGATTGTGATCAAGAAGAAGATGAAGAAAAGAAGAGAGAAAAAAGAAGGTGGATTTTTAGGAAACCAGTGAATCATGAAACAGTGAATAATACACAACAAAGTACACCAACAAAGTTGAAGCATGATGTTGTTAATGCTACAAGTGTGGCTTCAAGGACAGATCAGGATGAGAAACATGCTCTTGCTGTGGCTATGGCTACAGCTGAGGCAGCAAGAGCTACTGCTCAAGCTGCTGTGGAAGTTGCAAGGTTGACTAAGAGTTCAAATCATGCTAGGGAGCATTATGCTGCTGTTGTGGTTCAAACTTCTTTTAGAGGTTATTTGGCAAGAAGAGCACTTCGTGCGCTTAAGGGGTTAGTGAAGTTGCAAGCTTTAGTGAGAGGTCACAATGTTAGGAAGCAAGCAAAGATGACACTTAGGTGCATGCAAGCTCTTGTTAGAGTACAAGCAAGAGTTCTTGACCAAAGGATAAGGTCTACTTCACACGAAGGAAGTAGAAAATCAACCTTTAGTGACACTGCTAGTGTTTGGGATTCGCGGTATCTTCAAGACATTTCAGATAGGAAATCACTATCAAGAGAAGGAAGTAGTATTGCAGATGATTGGGATGATCGTCCACACACAGTTGAAGAAGTTAAGGCCATGTTGCAACAAAGGAAAGAAGCAGCAATGAAGAGGGAAAAGTCATTGTCCCAAGCATTTTCACAACAGATTTGGAGAAATGGTAGGACATCATCAATTGGAAATGAAGATGAACTTGAAGAGAGACCAAAATGGCTAGATAGATGGATGGCTACAAAACCATGGGAGAATAGGGGAAGAGCTTCAACTGATCAAAGAGACTCTATTAAGACTGTGGAAGTCGACACATCTCAACCTTATTCATATCTAGGAGCAAATTACAGAAGATCACATCCAAATTATCAATATAATCCACATCACCAACCACAAAGGCATTCCATTGCTTCACCGCTCCATAGATCACACCAAAATGGATCCGTTCACAACCAATCCATCACTACTCCGTCTCCAGCAAAATCAAGACCTATTCAAGTCCGGTCGTCAAGCCCTCGTTGTGTTCGAGAAGATAGAAGCTACCACCACACCTCTCAAACACCAAGCTTAAGGTCTAATTACCATTACAATGGGAACTTGTACCAAAATAGTAGGGGTGGAACAAGTAATGGTAGTAGTAACAGTATTGCTTCTACTACTGCTTCAGCTACATTGCCGAATTACATGCAAGCAACCGAGTCTGCAAAGGCTCGAATTCGGTCGCAGAGTGCGCCAAGGCAAAGGCCATCGACACCAGAGAGGGATAGATCCGGATCAGTAAAGAAAAGGCTTTCTTTCCCTGCTCCTGATCCTTATAATGTAGGAGGAGTTGGTTATGGAAATTATGGACATAGTTTAAGGAGTCCAAGTTTCAAGAGTGTGAATGTAGCAGCATCACATTATGGATTGGAGCAAAATTCCAATTACTCTTCTTGTTGTACTGAGAGTCTCGGTGGCGAGGTTTCACCTTCGTCAACCGGTGACCTTAGACGGTGGTTGAGATGA
Protein sequence

>Msa1236010

MGKKGSSSWLTAVKRAFRSPTKDSEKRSGRRREDCDQEEDEEKKREKRRWIFRKPVNHETVNNTQQSTPTKLKHDVVNATSVASRTDQDEKHALAVAMATAEAARATAQAAVEVARLTKSSNHAREHYAAVVVQTSFRGYLARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALVRVQARVLDQRIRSTSHEGSRKSTFSDTASVWDSRYLQDISDRKSLSREGSSIADDWDDRPHTVEEVKAMLQQRKEAAMKREKSLSQAFSQQIWRNGRTSSIGNEDELEERPKWLDRWMATKPWENRGRASTDQRDSIKTVEVDTSQPYSYLGANYRRSHPNYQYNPHHQPQRHSIASPLHRSHQNGSVHNQSITTPSPAKSRPIQVRSSSPRCVREDRSYHHTSQTPSLRSNYHYNGNLYQNSRGGTSNGSSNSIASTTASATLPNYMQATESAKARIRSQSAPRQRPSTPERDRSGSVKKRLSFPAPDPYNVGGVGYGNYGHSLRSPSFKSVNVAASHYGLEQNSNYSSCCTESLGGEVSPSSTGDLRRWLR*