AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0691020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691020 MtrunA17_Chr4g0039941 94.444 162 8 1 1 161 1 162 1.10e-106 301
Msa0691020 MtrunA17_Chr4g0039921 78.882 161 34 0 1 161 1 161 5.18e-90 259
Msa0691020 MtrunA17_Chr3g0100201 32.540 126 78 2 1 123 1 122 7.50e-16 70.5
Msa0691020 MtrunA17_Chr3g0100191 33.333 102 66 2 27 127 26 126 3.39e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTAACCCCATAATATATT+TGG 0.130140 4_4:-58967400 None:intergenic
GAAGGTGGATACTTAGAATT+TGG 0.200939 4_4:+58968651 Msa0691020:CDS
TAATAATCCCAAATATATTA+TGG 0.202677 4_4:+58967392 Msa0691020:five_prime_UTR
ATAGGTTATAAAATGATTAT+TGG 0.208282 4_4:+58969012 Msa0691020:three_prime_UTR
TGAGCCATTGGCTAACATTA+TGG 0.279776 4_4:+58968797 Msa0691020:CDS
GTTTCTGTTGAGGTAATTCT+TGG 0.289058 4_4:-58968826 None:intergenic
CTCTTTGGCACCATTGGTAT+TGG 0.293635 4_4:+58967572 Msa0691020:CDS
TAGGTTATAAAATGATTATT+GGG 0.324243 4_4:+58969013 Msa0691020:three_prime_UTR
TCCACCTTCTGAAACAATTC+AGG 0.368745 4_4:-58967617 None:intergenic
AATTTCTCTTTGTAACCAGC+TGG 0.370226 4_4:-58968582 None:intergenic
ACTAACCCCATAATATATTT+GGG 0.373584 4_4:-58967399 None:intergenic
AGATCCCATGCTTCACTTGC+TGG 0.373899 4_4:-58967551 None:intergenic
AATAATCCCAAATATATTAT+GGG 0.391799 4_4:+58967393 Msa0691020:five_prime_UTR
ATTTGTTTCCATTGAGCCAT+TGG 0.398190 4_4:+58968785 Msa0691020:CDS
TGCAATCTTGAGAAACATAT+AGG 0.420731 4_4:+58968994 Msa0691020:three_prime_UTR
TCTTTGTAACCAGCTGGGCC+AGG 0.438011 4_4:-58968576 None:intergenic
TGGGATCTCTTTGGCACCAT+TGG 0.444724 4_4:+58967566 Msa0691020:CDS
AGTGAAGCATGGGATCTCTT+TGG 0.449536 4_4:+58967557 Msa0691020:CDS
TTGTAGCCTTGTTTCTGTTG+AGG 0.460261 4_4:-58968836 None:intergenic
ATTGAAGGAGATGGAGGGAT+TGG 0.460943 4_4:+58967644 Msa0691020:CDS
TGGAAAAGAAAGAAGATGTC+TGG 0.469794 4_4:+58967497 Msa0691020:exon
ATGCTTCACTTGCTGGCACA+TGG 0.476940 4_4:-58967544 None:intergenic
CAGAAGGTGGAGCTAATTGA+AGG 0.500124 4_4:+58967629 Msa0691020:CDS
AATGTTAGCCAATGGCTCAA+TGG 0.500199 4_4:-58968793 None:intergenic
GCCTGAATTGTTTCAGAAGG+TGG 0.512921 4_4:+58967616 Msa0691020:CDS
GTGGAGCTAATTGAAGGAGA+TGG 0.515580 4_4:+58967635 Msa0691020:CDS
AAAGAAGCAGAGGTAGTTGA+AGG 0.515702 4_4:+58968633 Msa0691020:CDS
ACAGGTACACCTGGCCCAGC+TGG 0.517233 4_4:+58968567 Msa0691020:intron
AATGGAAACAAATGAGGCAT+TGG 0.521286 4_4:-58968775 None:intergenic
ATTGCTAATATATTAATGTA+TGG 0.555175 4_4:-58967432 None:intergenic
GAGCTAATTGAAGGAGATGG+AGG 0.556743 4_4:+58967638 Msa0691020:CDS
ATTTCTCTTTGTAACCAGCT+GGG 0.558097 4_4:-58968581 None:intergenic
ATGTGCCAGCAAGTGAAGCA+TGG 0.558793 4_4:+58967546 Msa0691020:CDS
CTCAAACTCACATTCACTCC+AGG 0.561646 4_4:+58967674 Msa0691020:CDS
GTCTCATTGGACTCAACGTA+AGG 0.570544 4_4:+58967366 None:intergenic
CTACCTCTGCTTCTTTGATG+CGG 0.573581 4_4:-58968626 None:intergenic
TGTGCCAGCAAGTGAAGCAT+GGG 0.578830 4_4:+58967547 Msa0691020:CDS
TCTAATCAAGCAAATGCACT+AGG 0.583851 4_4:-58969041 None:intergenic
GGAGTTTGAACAGGTACACC+TGG 0.591936 4_4:+58968558 Msa0691020:intron
TGGCTCAATGGAAACAAATG+AGG 0.595922 4_4:-58968781 None:intergenic
GAAGCAGAGGTAGTTGAAGG+TGG 0.597763 4_4:+58968636 Msa0691020:CDS
AGCTAATTGAAGGAGATGGA+GGG 0.610505 4_4:+58967639 Msa0691020:CDS
ATAATCCCAAATATATTATG+GGG 0.626296 4_4:+58967394 Msa0691020:five_prime_UTR
GATGCCTGAATTGTTTCAGA+AGG 0.636610 4_4:+58967613 Msa0691020:CDS
ACTTCCATAATGTTAGCCAA+TGG 0.636775 4_4:-58968801 None:intergenic
AAACCGCATCAAAGAAGCAG+AGG 0.668061 4_4:+58968623 Msa0691020:CDS
TGGTATTGGAAAACTCGTTG+AGG 0.673789 4_4:+58967586 Msa0691020:CDS
TCATCTGAGAAATAATACAA+AGG 0.684644 4_4:-58968969 None:intergenic
GAATTACCTCAACAGAAACA+AGG 0.699162 4_4:+58968830 Msa0691020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTGTGTTTTTTTTTAT+AGG - chr4_4:58968509-58968528 None:intergenic 10.0%
!!! TTAATTGTGTTTTTTTTTAT+AGG - chr4_4:58968509-58968528 None:intergenic 10.0%
!! TAATAATCCCAAATATATTA+TGG + chr4_4:58967392-58967411 Msa0691020:five_prime_UTR 15.0%
!! AATAATCCCAAATATATTAT+GGG + chr4_4:58967393-58967412 Msa0691020:five_prime_UTR 15.0%
!! ATTGCTAATATATTAATGTA+TGG - chr4_4:58967435-58967454 None:intergenic 15.0%
!! CACATGATTATTATATATTT+CGG + chr4_4:58967845-58967864 Msa0691020:intron 15.0%
!! ACATGATTATTATATATTTC+GGG + chr4_4:58967846-58967865 Msa0691020:intron 15.0%
!! ATAGGTTATAAAATGATTAT+TGG + chr4_4:58969012-58969031 Msa0691020:three_prime_UTR 15.0%
!! TAGGTTATAAAATGATTATT+GGG + chr4_4:58969013-58969032 Msa0691020:three_prime_UTR 15.0%
!! TAATAATCCCAAATATATTA+TGG + chr4_4:58967392-58967411 Msa0691020:five_prime_UTR 15.0%
!! AATAATCCCAAATATATTAT+GGG + chr4_4:58967393-58967412 Msa0691020:five_prime_UTR 15.0%
!! ATTGCTAATATATTAATGTA+TGG - chr4_4:58967435-58967454 None:intergenic 15.0%
!! CACATGATTATTATATATTT+CGG + chr4_4:58967845-58967864 Msa0691020:intron 15.0%
!! ACATGATTATTATATATTTC+GGG + chr4_4:58967846-58967865 Msa0691020:intron 15.0%
!! ATAGGTTATAAAATGATTAT+TGG + chr4_4:58969012-58969031 Msa0691020:three_prime_UTR 15.0%
!! TAGGTTATAAAATGATTATT+GGG + chr4_4:58969013-58969032 Msa0691020:three_prime_UTR 15.0%
!! ATAATCCCAAATATATTATG+GGG + chr4_4:58967394-58967413 Msa0691020:five_prime_UTR 20.0%
!!! TCAAAAGAGTAAATATTTTG+TGG + chr4_4:58967477-58967496 Msa0691020:five_prime_UTR 20.0%
!! TAAATAATATATGAATGACC+TGG - chr4_4:58967695-58967714 None:intergenic 20.0%
!!! TTTTTATGTATTGTTGTGTT+AGG - chr4_4:58967739-58967758 None:intergenic 20.0%
!! TGTATATGTGTATGTATTAT+AGG - chr4_4:58967762-58967781 None:intergenic 20.0%
!! AAATTCAATAAGTGTATGTT+TGG + chr4_4:58968050-58968069 Msa0691020:intron 20.0%
!!! AGAAACATAACTTCTTATTT+CGG + chr4_4:58968100-58968119 Msa0691020:intron 20.0%
!! AATACTGAATACAAATCTAA+AGG + chr4_4:58968460-58968479 Msa0691020:intron 20.0%
!!! TTGTGTTTTTTTTTATAGGA+GGG - chr4_4:58968505-58968524 None:intergenic 20.0%
!!! ATTGTGTTTTTTTTTATAGG+AGG - chr4_4:58968506-58968525 None:intergenic 20.0%
!! ATAATCCCAAATATATTATG+GGG + chr4_4:58967394-58967413 Msa0691020:five_prime_UTR 20.0%
!!! TCAAAAGAGTAAATATTTTG+TGG + chr4_4:58967477-58967496 Msa0691020:five_prime_UTR 20.0%
!! TAAATAATATATGAATGACC+TGG - chr4_4:58967695-58967714 None:intergenic 20.0%
!!! TTTTTATGTATTGTTGTGTT+AGG - chr4_4:58967739-58967758 None:intergenic 20.0%
!! TGTATATGTGTATGTATTAT+AGG - chr4_4:58967762-58967781 None:intergenic 20.0%
!! AAATTCAATAAGTGTATGTT+TGG + chr4_4:58968050-58968069 Msa0691020:intron 20.0%
!!! AGAAACATAACTTCTTATTT+CGG + chr4_4:58968100-58968119 Msa0691020:intron 20.0%
!! AATACTGAATACAAATCTAA+AGG + chr4_4:58968460-58968479 Msa0691020:intron 20.0%
!!! TTGTGTTTTTTTTTATAGGA+GGG - chr4_4:58968505-58968524 None:intergenic 20.0%
!!! ATTGTGTTTTTTTTTATAGG+AGG - chr4_4:58968506-58968525 None:intergenic 20.0%
! ACTAACCCCATAATATATTT+GGG - chr4_4:58967402-58967421 None:intergenic 25.0%
! TACTAACCCCATAATATATT+TGG - chr4_4:58967403-58967422 None:intergenic 25.0%
!!! CTCTTTTGAGTTTTAAAATG+TGG - chr4_4:58967466-58967485 None:intergenic 25.0%
! TATATATTTCGGGAAAAATC+TGG + chr4_4:58967856-58967875 Msa0691020:intron 25.0%
! AGCTACAAAATCATATTAGA+AGG - chr4_4:58968201-58968220 None:intergenic 25.0%
!!! AATATATTTTCTTTGGGACA+CGG - chr4_4:58968418-58968437 None:intergenic 25.0%
!!! TGTGTTTTTTTTTATAGGAG+GGG - chr4_4:58968504-58968523 None:intergenic 25.0%
!!! GTAACATTGATTGTTTTGAT+TGG + chr4_4:58968537-58968556 Msa0691020:intron 25.0%
!!! CGTTTTGAAATAATAGAGAA+AGG + chr4_4:58968693-58968712 Msa0691020:CDS 25.0%
! TCATCTGAGAAATAATACAA+AGG - chr4_4:58968972-58968991 None:intergenic 25.0%
! ACTAACCCCATAATATATTT+GGG - chr4_4:58967402-58967421 None:intergenic 25.0%
! TACTAACCCCATAATATATT+TGG - chr4_4:58967403-58967422 None:intergenic 25.0%
!!! CTCTTTTGAGTTTTAAAATG+TGG - chr4_4:58967466-58967485 None:intergenic 25.0%
! TATATATTTCGGGAAAAATC+TGG + chr4_4:58967856-58967875 Msa0691020:intron 25.0%
! AGCTACAAAATCATATTAGA+AGG - chr4_4:58968201-58968220 None:intergenic 25.0%
!!! AATATATTTTCTTTGGGACA+CGG - chr4_4:58968418-58968437 None:intergenic 25.0%
!!! TGTGTTTTTTTTTATAGGAG+GGG - chr4_4:58968504-58968523 None:intergenic 25.0%
!!! GTAACATTGATTGTTTTGAT+TGG + chr4_4:58968537-58968556 Msa0691020:intron 25.0%
!!! CGTTTTGAAATAATAGAGAA+AGG + chr4_4:58968693-58968712 Msa0691020:CDS 25.0%
! TCATCTGAGAAATAATACAA+AGG - chr4_4:58968972-58968991 None:intergenic 25.0%
ACAACATGTCTATGTACAAA+TGG + chr4_4:58967944-58967963 Msa0691020:intron 30.0%
! TGAGCAGAAAAAGAAACTTT+TGG + chr4_4:58968027-58968046 Msa0691020:intron 30.0%
! CAACTTCTAGTTATAAGTTG+TGG - chr4_4:58968163-58968182 None:intergenic 30.0%
TTAAAACAATCAACCACAAG+TGG + chr4_4:58968318-58968337 Msa0691020:intron 30.0%
! GCGGTTTTCATTATCAATTT+TGG - chr4_4:58968610-58968629 None:intergenic 30.0%
TGCAATCTTGAGAAACATAT+AGG + chr4_4:58968994-58969013 Msa0691020:three_prime_UTR 30.0%
ACAACATGTCTATGTACAAA+TGG + chr4_4:58967944-58967963 Msa0691020:intron 30.0%
! TGAGCAGAAAAAGAAACTTT+TGG + chr4_4:58968027-58968046 Msa0691020:intron 30.0%
! CAACTTCTAGTTATAAGTTG+TGG - chr4_4:58968163-58968182 None:intergenic 30.0%
TTAAAACAATCAACCACAAG+TGG + chr4_4:58968318-58968337 Msa0691020:intron 30.0%
! GCGGTTTTCATTATCAATTT+TGG - chr4_4:58968610-58968629 None:intergenic 30.0%
TGCAATCTTGAGAAACATAT+AGG + chr4_4:58968994-58969013 Msa0691020:three_prime_UTR 30.0%
TGGAAAAGAAAGAAGATGTC+TGG + chr4_4:58967497-58967516 Msa0691020:exon 35.0%
! AACGAGTTTTCCAATACCAA+TGG - chr4_4:58967585-58967604 None:intergenic 35.0%
CCAAATAGTGAGTCAATTGA+TGG - chr4_4:58967813-58967832 None:intergenic 35.0%
! CCATCAATTGACTCACTATT+TGG + chr4_4:58967810-58967829 Msa0691020:intron 35.0%
ACATGTCTATGTACAAATGG+AGG + chr4_4:58967947-58967966 Msa0691020:intron 35.0%
CATGTCTATGTACAAATGGA+GGG + chr4_4:58967948-58967967 Msa0691020:intron 35.0%
ATAAGTTGTGGAGACAAAGA+CGG - chr4_4:58968151-58968170 None:intergenic 35.0%
!!! GTTTTGATTGGAGTTTGAAC+AGG + chr4_4:58968549-58968568 Msa0691020:intron 35.0%
ATTTCTCTTTGTAACCAGCT+GGG - chr4_4:58968584-58968603 None:intergenic 35.0%
AATTTCTCTTTGTAACCAGC+TGG - chr4_4:58968585-58968604 None:intergenic 35.0%
GAAGGTGGATACTTAGAATT+TGG + chr4_4:58968651-58968670 Msa0691020:CDS 35.0%
AATGGAAACAAATGAGGCAT+TGG - chr4_4:58968778-58968797 None:intergenic 35.0%
ATTTGTTTCCATTGAGCCAT+TGG + chr4_4:58968785-58968804 Msa0691020:CDS 35.0%
ACTTCCATAATGTTAGCCAA+TGG - chr4_4:58968804-58968823 None:intergenic 35.0%
GTTTCTGTTGAGGTAATTCT+TGG - chr4_4:58968829-58968848 None:intergenic 35.0%
GAATTACCTCAACAGAAACA+AGG + chr4_4:58968830-58968849 Msa0691020:CDS 35.0%
TCTAATCAAGCAAATGCACT+AGG - chr4_4:58969044-58969063 None:intergenic 35.0%
TGGAAAAGAAAGAAGATGTC+TGG + chr4_4:58967497-58967516 Msa0691020:exon 35.0%
! AACGAGTTTTCCAATACCAA+TGG - chr4_4:58967585-58967604 None:intergenic 35.0%
CCAAATAGTGAGTCAATTGA+TGG - chr4_4:58967813-58967832 None:intergenic 35.0%
! CCATCAATTGACTCACTATT+TGG + chr4_4:58967810-58967829 Msa0691020:intron 35.0%
ACATGTCTATGTACAAATGG+AGG + chr4_4:58967947-58967966 Msa0691020:intron 35.0%
CATGTCTATGTACAAATGGA+GGG + chr4_4:58967948-58967967 Msa0691020:intron 35.0%
ATAAGTTGTGGAGACAAAGA+CGG - chr4_4:58968151-58968170 None:intergenic 35.0%
!!! GTTTTGATTGGAGTTTGAAC+AGG + chr4_4:58968549-58968568 Msa0691020:intron 35.0%
ATTTCTCTTTGTAACCAGCT+GGG - chr4_4:58968584-58968603 None:intergenic 35.0%
AATTTCTCTTTGTAACCAGC+TGG - chr4_4:58968585-58968604 None:intergenic 35.0%
GAAGGTGGATACTTAGAATT+TGG + chr4_4:58968651-58968670 Msa0691020:CDS 35.0%
AATGGAAACAAATGAGGCAT+TGG - chr4_4:58968778-58968797 None:intergenic 35.0%
ATTTGTTTCCATTGAGCCAT+TGG + chr4_4:58968785-58968804 Msa0691020:CDS 35.0%
ACTTCCATAATGTTAGCCAA+TGG - chr4_4:58968804-58968823 None:intergenic 35.0%
GTTTCTGTTGAGGTAATTCT+TGG - chr4_4:58968829-58968848 None:intergenic 35.0%
GAATTACCTCAACAGAAACA+AGG + chr4_4:58968830-58968849 Msa0691020:CDS 35.0%
TCTAATCAAGCAAATGCACT+AGG - chr4_4:58969044-58969063 None:intergenic 35.0%
! TGGTATTGGAAAACTCGTTG+AGG + chr4_4:58967586-58967605 Msa0691020:CDS 40.0%
GATGCCTGAATTGTTTCAGA+AGG + chr4_4:58967613-58967632 Msa0691020:CDS 40.0%
!! TCCACCTTCTGAAACAATTC+AGG - chr4_4:58967620-58967639 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_4:58967639-58967658 Msa0691020:CDS 40.0%
AATCTAGAGATTCGTGCGTA+TGG + chr4_4:58967985-58968004 Msa0691020:intron 40.0%
CACAAGTGGAGCTATCAAAA+GGG + chr4_4:58968332-58968351 Msa0691020:intron 40.0%
! TCAAAAGGGTGGTCTTGATT+AGG + chr4_4:58968346-58968365 Msa0691020:intron 40.0%
AGGTTGACACCACTCTAATT+CGG + chr4_4:58968366-58968385 Msa0691020:intron 40.0%
! GGACACGGTAATTGTTGATT+TGG - chr4_4:58968403-58968422 None:intergenic 40.0%
AAAGAAGCAGAGGTAGTTGA+AGG + chr4_4:58968633-58968652 Msa0691020:CDS 40.0%
TGGCTCAATGGAAACAAATG+AGG - chr4_4:58968784-58968803 None:intergenic 40.0%
AATGTTAGCCAATGGCTCAA+TGG - chr4_4:58968796-58968815 None:intergenic 40.0%
! TGAGCCATTGGCTAACATTA+TGG + chr4_4:58968797-58968816 Msa0691020:CDS 40.0%
!! TTGTAGCCTTGTTTCTGTTG+AGG - chr4_4:58968839-58968858 None:intergenic 40.0%
! TGGTATTGGAAAACTCGTTG+AGG + chr4_4:58967586-58967605 Msa0691020:CDS 40.0%
GATGCCTGAATTGTTTCAGA+AGG + chr4_4:58967613-58967632 Msa0691020:CDS 40.0%
!! TCCACCTTCTGAAACAATTC+AGG - chr4_4:58967620-58967639 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_4:58967639-58967658 Msa0691020:CDS 40.0%
AATCTAGAGATTCGTGCGTA+TGG + chr4_4:58967985-58968004 Msa0691020:intron 40.0%
CACAAGTGGAGCTATCAAAA+GGG + chr4_4:58968332-58968351 Msa0691020:intron 40.0%
! TCAAAAGGGTGGTCTTGATT+AGG + chr4_4:58968346-58968365 Msa0691020:intron 40.0%
AGGTTGACACCACTCTAATT+CGG + chr4_4:58968366-58968385 Msa0691020:intron 40.0%
! GGACACGGTAATTGTTGATT+TGG - chr4_4:58968403-58968422 None:intergenic 40.0%
AAAGAAGCAGAGGTAGTTGA+AGG + chr4_4:58968633-58968652 Msa0691020:CDS 40.0%
TGGCTCAATGGAAACAAATG+AGG - chr4_4:58968784-58968803 None:intergenic 40.0%
AATGTTAGCCAATGGCTCAA+TGG - chr4_4:58968796-58968815 None:intergenic 40.0%
! TGAGCCATTGGCTAACATTA+TGG + chr4_4:58968797-58968816 Msa0691020:CDS 40.0%
!! TTGTAGCCTTGTTTCTGTTG+AGG - chr4_4:58968839-58968858 None:intergenic 40.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_4:58967557-58967576 Msa0691020:CDS 45.0%
CTCTTTGGCACCATTGGTAT+TGG + chr4_4:58967572-58967591 Msa0691020:CDS 45.0%
GCCTGAATTGTTTCAGAAGG+TGG + chr4_4:58967616-58967635 Msa0691020:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_4:58967629-58967648 Msa0691020:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_4:58967635-58967654 Msa0691020:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_4:58967638-58967657 Msa0691020:CDS 45.0%
ATTGAAGGAGATGGAGGGAT+TGG + chr4_4:58967644-58967663 Msa0691020:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_4:58967674-58967693 Msa0691020:CDS 45.0%
!! CCTTTTGATAGCTCCACTTG+TGG - chr4_4:58968334-58968353 None:intergenic 45.0%
CCACAAGTGGAGCTATCAAA+AGG + chr4_4:58968331-58968350 Msa0691020:intron 45.0%
AAGTGGAGCTATCAAAAGGG+TGG + chr4_4:58968335-58968354 Msa0691020:intron 45.0%
GAGTGTCAACCGAATTAGAG+TGG - chr4_4:58968378-58968397 None:intergenic 45.0%
AAACCGCATCAAAGAAGCAG+AGG + chr4_4:58968623-58968642 Msa0691020:CDS 45.0%
CTACCTCTGCTTCTTTGATG+CGG - chr4_4:58968629-58968648 None:intergenic 45.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_4:58967557-58967576 Msa0691020:CDS 45.0%
CTCTTTGGCACCATTGGTAT+TGG + chr4_4:58967572-58967591 Msa0691020:CDS 45.0%
GCCTGAATTGTTTCAGAAGG+TGG + chr4_4:58967616-58967635 Msa0691020:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_4:58967629-58967648 Msa0691020:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_4:58967635-58967654 Msa0691020:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_4:58967638-58967657 Msa0691020:CDS 45.0%
ATTGAAGGAGATGGAGGGAT+TGG + chr4_4:58967644-58967663 Msa0691020:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_4:58967674-58967693 Msa0691020:CDS 45.0%
!! CCTTTTGATAGCTCCACTTG+TGG - chr4_4:58968334-58968353 None:intergenic 45.0%
CCACAAGTGGAGCTATCAAA+AGG + chr4_4:58968331-58968350 Msa0691020:intron 45.0%
AAGTGGAGCTATCAAAAGGG+TGG + chr4_4:58968335-58968354 Msa0691020:intron 45.0%
GAGTGTCAACCGAATTAGAG+TGG - chr4_4:58968378-58968397 None:intergenic 45.0%
AAACCGCATCAAAGAAGCAG+AGG + chr4_4:58968623-58968642 Msa0691020:CDS 45.0%
CTACCTCTGCTTCTTTGATG+CGG - chr4_4:58968629-58968648 None:intergenic 45.0%
!!! TTTATTAATATATTTTCTTT+GGG - chr4_4:58968424-58968443 None:intergenic 5.0%
!!! TTTTATTAATATATTTTCTT+TGG - chr4_4:58968425-58968444 None:intergenic 5.0%
!! AAAGAAAATATATTAATAAA+AGG + chr4_4:58968424-58968443 Msa0691020:intron 5.0%
!!! TTTATTAATATATTTTCTTT+GGG - chr4_4:58968424-58968443 None:intergenic 5.0%
!!! TTTTATTAATATATTTTCTT+TGG - chr4_4:58968425-58968444 None:intergenic 5.0%
!! AAAGAAAATATATTAATAAA+AGG + chr4_4:58968424-58968443 Msa0691020:intron 5.0%
! ATGCTTCACTTGCTGGCACA+TGG - chr4_4:58967547-58967566 None:intergenic 50.0%
ATGTGCCAGCAAGTGAAGCA+TGG + chr4_4:58967546-58967565 Msa0691020:CDS 50.0%
TGTGCCAGCAAGTGAAGCAT+GGG + chr4_4:58967547-58967566 Msa0691020:CDS 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_4:58967554-58967573 None:intergenic 50.0%
TGGGATCTCTTTGGCACCAT+TGG + chr4_4:58967566-58967585 Msa0691020:CDS 50.0%
GGAGTTTGAACAGGTACACC+TGG + chr4_4:58968558-58968577 Msa0691020:intron 50.0%
GAAGCAGAGGTAGTTGAAGG+TGG + chr4_4:58968636-58968655 Msa0691020:CDS 50.0%
! ATGCTTCACTTGCTGGCACA+TGG - chr4_4:58967547-58967566 None:intergenic 50.0%
ATGTGCCAGCAAGTGAAGCA+TGG + chr4_4:58967546-58967565 Msa0691020:CDS 50.0%
TGTGCCAGCAAGTGAAGCAT+GGG + chr4_4:58967547-58967566 Msa0691020:CDS 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_4:58967554-58967573 None:intergenic 50.0%
TGGGATCTCTTTGGCACCAT+TGG + chr4_4:58967566-58967585 Msa0691020:CDS 50.0%
GGAGTTTGAACAGGTACACC+TGG + chr4_4:58968558-58968577 Msa0691020:intron 50.0%
GAAGCAGAGGTAGTTGAAGG+TGG + chr4_4:58968636-58968655 Msa0691020:CDS 50.0%
TCTTTGTAACCAGCTGGGCC+AGG - chr4_4:58968579-58968598 None:intergenic 55.0%
TCTTTGTAACCAGCTGGGCC+AGG - chr4_4:58968579-58968598 None:intergenic 55.0%
ACAGGTACACCTGGCCCAGC+TGG + chr4_4:58968567-58968586 Msa0691020:intron 65.0%
ACAGGTACACCTGGCCCAGC+TGG + chr4_4:58968567-58968586 Msa0691020:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 58967380 58969101 58967380 ID=Msa0691020;Name=Msa0691020
chr4_4 mRNA 58967380 58969101 58967380 ID=Msa0691020-mRNA-1;Parent=Msa0691020;Name=Msa0691020-mRNA-1;_AED=0.28;_eAED=0.28;_QI=132|1|1|1|1|1|2|229|161
chr4_4 exon 58967380 58967695 58967380 ID=Msa0691020-mRNA-1:exon:15940;Parent=Msa0691020-mRNA-1
chr4_4 exon 58968571 58969101 58968571 ID=Msa0691020-mRNA-1:exon:15941;Parent=Msa0691020-mRNA-1
chr4_4 five_prime_UTR 58967380 58967511 58967380 ID=Msa0691020-mRNA-1:five_prime_utr;Parent=Msa0691020-mRNA-1
chr4_4 CDS 58967512 58967695 58967512 ID=Msa0691020-mRNA-1:cds;Parent=Msa0691020-mRNA-1
chr4_4 CDS 58968571 58968872 58968571 ID=Msa0691020-mRNA-1:cds;Parent=Msa0691020-mRNA-1
chr4_4 three_prime_UTR 58968873 58969101 58968873 ID=Msa0691020-mRNA-1:three_prime_utr;Parent=Msa0691020-mRNA-1
Gene Sequence

>Msa0691020

ATGTCTGGTCAACTTGAGCATGAGTTAGAGCTCCATGTGCCAGCAAGTGAAGCATGGGATCTCTTTGGCACCATTGGTATTGGAAAACTCGTTGAGGCTGAGATGCCTGAATTGTTTCAGAAGGTGGAGCTAATTGAAGGAGATGGAGGGATTGGAACTATTCTCAAACTCACATTCACTCCAGGTACACCTGGCCCAGCTGGTTACAAAGAGAAATTCACCAAAATTGATAATGAAAACCGCATCAAAGAAGCAGAGGTAGTTGAAGGTGGATACTTAGAATTTGGATTCACACTATTTAGAGTTCGTTTTGAAATAATAGAGAAAGGTGAAGATTCAAGCATAATCAAAACTACAATAGAGTATGAAATCAAAGAAGAGCATGCAGCCAATGCCTCATTTGTTTCCATTGAGCCATTGGCTAACATTATGGAAGTTGCCAAGAATTACCTCAACAGAAACAAGGCTACAAAAGAAGCAAAGTAG
Protein sequence

>Msa0691020

MSGQLEHELELHVPASEAWDLFGTIGIGKLVEAEMPELFQKVELIEGDGGIGTILKLTFTPGTPGPAGYKEKFTKIDNENRIKEAEVVEGGYLEFGFTLFRVRFEIIEKGEDSSIIKTTIEYEIKEEHAANASFVSIEPLANIMEVAKNYLNRNKATKEAK*