AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1073830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1073830 MtrunA17_Chr7g0256851 98.261 115 2 0 1 115 1 115 1.32e-80 231
Msa1073830 MtrunA17_Chr3g0085831 50.476 105 48 3 1 102 1 104 9.21e-30 105
Msa1073830 MtrunA17_Chr5g0437861 44.595 74 37 2 1 71 1 73 1.29e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1073830 AT2G41430.5 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1073830 AT2G41430.4 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1073830 AT2G41430.2 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1073830 AT2G41430.1 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1073830 AT2G41430.3 65.000 80 24 2 1 76 1 80 4.23e-30 104
Msa1073830 AT4G14270.1 57.407 54 23 0 2 55 3 56 2.36e-17 72.8
Msa1073830 AT4G14270.2 57.407 54 23 0 2 55 3 56 2.36e-17 72.8

Find 41 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGGTAAGAAAGTTTA+TGG 0.140300 7_2:-71468020 None:intergenic
AAGGAATTACCAGAGAAATT+TGG 0.241148 7_2:-71466739 None:intergenic
TTTCTTACCTGTCTTCAAAA+TGG 0.243639 7_2:+71468028 Msa1073830:three_prime_UTR
ATTGGTCAATTGATTGCTTC+TGG 0.249156 7_2:-71468127 None:intergenic
TTCAAGATGGCATTAGTTTC+AGG 0.269984 7_2:+71467263 Msa1073830:exon
TTCCTTCTTACCAAAATCTA+TGG 0.308472 7_2:+71467871 Msa1073830:three_prime_UTR
CTTTCTGTAACTTCTTGTAT+AGG 0.333956 7_2:+71467827 Msa1073830:three_prime_UTR
GGAATGTAAAGTGGTGCATT+AGG 0.338731 7_2:-71467305 None:intergenic
GACGGTCTTGCCTTAGCTTT+CGG 0.365977 7_2:+71467773 Msa1073830:three_prime_UTR
CAAGGAGCAAAGAAATTTAC+TGG 0.372256 7_2:+71467701 Msa1073830:CDS
AGTAAGTTTATTATTACAAT+TGG 0.386330 7_2:-71468145 None:intergenic
GTCACCAGCTGCCACCATTC+CGG 0.393504 7_2:-71467359 None:intergenic
TGAAATGGTACCATGATTAC+TGG 0.394842 7_2:+71467387 Msa1073830:CDS
AGCCTTCATCATCATGTTGC+TGG 0.408811 7_2:-71467415 None:intergenic
ACCGTCGCATTCAGCGTCTA+TGG 0.413218 7_2:-71467756 None:intergenic
GAATGTAAAGTGGTGCATTA+GGG 0.425842 7_2:-71467304 None:intergenic
AGACAGGTAAGAAAGTTTAT+GGG 0.447864 7_2:-71468019 None:intergenic
TCTCACTAACCAAATTTCTC+TGG 0.457260 7_2:+71466730 Msa1073830:five_prime_UTR
TTCTCCGGAATGGTGGCAGC+TGG 0.463269 7_2:+71467355 Msa1073830:CDS
GCTTCTGGACATGTATTGTA+TGG 0.492883 7_2:-71468112 None:intergenic
TCGACATAGCGAAATGCAGC+TGG 0.498421 7_2:-71467326 None:intergenic
GCATCAAGATCAAACGCATC+AGG 0.498917 7_2:-71467488 None:intergenic
GCTGGCTAAGCCAGTAATCA+TGG 0.519480 7_2:-71467397 None:intergenic
ATTTACTGGATGAGCTAAAG+AGG 0.519766 7_2:+71467715 Msa1073830:exon
TGTAACTTCTTGTATAGGCA+AGG 0.531546 7_2:+71467832 Msa1073830:three_prime_UTR
TGACAGAACTCAGAAGTGTA+TGG 0.537723 7_2:+71468077 Msa1073830:three_prime_UTR
AGCTGGTGACAACACTGAAA+TGG 0.546239 7_2:+71467372 Msa1073830:CDS
AGCTGCATTTCGCTATGTCG+AGG 0.547833 7_2:+71467328 Msa1073830:CDS
GATGATGTAGATGAGTTTGA+CGG 0.549584 7_2:+71467443 Msa1073830:CDS
AGCCAGCAACATGATGATGA+AGG 0.560493 7_2:+71467413 Msa1073830:CDS
AGCTAAATAACCGAAAGCTA+AGG 0.582950 7_2:-71467783 None:intergenic
ACCATAGACGCTGAATGCGA+CGG 0.583100 7_2:+71467755 Msa1073830:three_prime_UTR
AGAGTTTATCAAGTCTTCTG+AGG 0.595138 7_2:+71467544 Msa1073830:CDS
ATCAAGTCTTCTGAGGCACA+AGG 0.619098 7_2:+71467551 Msa1073830:CDS
AGGGTGAAGGTGATTCAAGA+TGG 0.622570 7_2:+71467250 Msa1073830:exon
AAATCTATGGTAAAATAGAG+AGG 0.627618 7_2:+71467884 Msa1073830:three_prime_UTR
AAGATGGCATTAGTTTCAGG+AGG 0.635369 7_2:+71467266 Msa1073830:exon
TGATGCGTTTGATCTTGATG+CGG 0.644823 7_2:+71467490 Msa1073830:CDS
TATAACAGCTCTTACAACAT+CGG 0.657333 7_2:-71467943 None:intergenic
AATGCAGCTGGAATGTAAAG+TGG 0.663728 7_2:-71467314 None:intergenic
ACAGAAAGTTAAAACTGACA+TGG 0.696360 7_2:-71467812 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATGGTTATTTTACAAT+TGG - chr7_2:71467018-71467037 None:intergenic 15.0%
!!! AATTATGGTTATTTTACAAT+TGG - chr7_2:71467018-71467037 None:intergenic 15.0%
!!! CATTGTTAATTGTTGATTAA+TGG + chr7_2:71467204-71467223 Msa1073830:intron 20.0%
!!! CATTGTTAATTGTTGATTAA+TGG + chr7_2:71467204-71467223 Msa1073830:intron 20.0%
! TGAGTAATTGAAAGAGAAAA+AGG - chr7_2:71466761-71466780 None:intergenic 25.0%
! TCATGATGATTCATCAATTA+TGG - chr7_2:71467033-71467052 None:intergenic 25.0%
! TTGATTAATGGTGTAGATTA+AGG + chr7_2:71467216-71467235 Msa1073830:intron 25.0%
! AGATTAAGGAAAAAGTGATA+AGG + chr7_2:71467230-71467249 Msa1073830:intron 25.0%
! GATTAAGGAAAAAGTGATAA+GGG + chr7_2:71467231-71467250 Msa1073830:intron 25.0%
!! AACTTTCCTCTGAAAAATAA+CGG - chr7_2:71467673-71467692 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG - chr7_2:71467884-71467903 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG + chr7_2:71467884-71467903 Msa1073830:three_prime_UTR 25.0%
! TGAGTAATTGAAAGAGAAAA+AGG - chr7_2:71466761-71466780 None:intergenic 25.0%
! TCATGATGATTCATCAATTA+TGG - chr7_2:71467033-71467052 None:intergenic 25.0%
! TTGATTAATGGTGTAGATTA+AGG + chr7_2:71467216-71467235 Msa1073830:intron 25.0%
! AGATTAAGGAAAAAGTGATA+AGG + chr7_2:71467230-71467249 Msa1073830:intron 25.0%
! GATTAAGGAAAAAGTGATAA+GGG + chr7_2:71467231-71467250 Msa1073830:intron 25.0%
!! AACTTTCCTCTGAAAAATAA+CGG - chr7_2:71467673-71467692 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG - chr7_2:71467884-71467903 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG + chr7_2:71467884-71467903 Msa1073830:three_prime_UTR 25.0%
AAGGAATTACCAGAGAAATT+TGG - chr7_2:71466742-71466761 None:intergenic 30.0%
!!! AACATACCGTTATTTTTCAG+AGG + chr7_2:71467664-71467683 Msa1073830:intron 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG + chr7_2:71467676-71467695 Msa1073830:intron 30.0%
ACAGAAAGTTAAAACTGACA+TGG - chr7_2:71467815-71467834 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG + chr7_2:71467827-71467846 Msa1073830:three_prime_UTR 30.0%
TTCCTTCTTACCAAAATCTA+TGG + chr7_2:71467871-71467890 Msa1073830:three_prime_UTR 30.0%
!! TACCATAGATTTTGGTAAGA+AGG - chr7_2:71467876-71467895 None:intergenic 30.0%
TATAACAGCTCTTACAACAT+CGG - chr7_2:71467946-71467965 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG - chr7_2:71468022-71468041 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG - chr7_2:71468023-71468042 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG + chr7_2:71468028-71468047 Msa1073830:three_prime_UTR 30.0%
AAGGAATTACCAGAGAAATT+TGG - chr7_2:71466742-71466761 None:intergenic 30.0%
!!! AACATACCGTTATTTTTCAG+AGG + chr7_2:71467664-71467683 Msa1073830:intron 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG + chr7_2:71467676-71467695 Msa1073830:intron 30.0%
ACAGAAAGTTAAAACTGACA+TGG - chr7_2:71467815-71467834 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG + chr7_2:71467827-71467846 Msa1073830:three_prime_UTR 30.0%
TTCCTTCTTACCAAAATCTA+TGG + chr7_2:71467871-71467890 Msa1073830:three_prime_UTR 30.0%
!! TACCATAGATTTTGGTAAGA+AGG - chr7_2:71467876-71467895 None:intergenic 30.0%
TATAACAGCTCTTACAACAT+CGG - chr7_2:71467946-71467965 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG - chr7_2:71468022-71468041 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG - chr7_2:71468023-71468042 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG + chr7_2:71468028-71468047 Msa1073830:three_prime_UTR 30.0%
TCTCACTAACCAAATTTCTC+TGG + chr7_2:71466730-71466749 Msa1073830:five_prime_UTR 35.0%
! TTCAAGATGGCATTAGTTTC+AGG + chr7_2:71467263-71467282 Msa1073830:exon 35.0%
GAATGTAAAGTGGTGCATTA+GGG - chr7_2:71467307-71467326 None:intergenic 35.0%
TGAAATGGTACCATGATTAC+TGG + chr7_2:71467387-71467406 Msa1073830:CDS 35.0%
GATGATGTAGATGAGTTTGA+CGG + chr7_2:71467443-71467462 Msa1073830:CDS 35.0%
AGAGTTTATCAAGTCTTCTG+AGG + chr7_2:71467544-71467563 Msa1073830:CDS 35.0%
CAAGGAGCAAAGAAATTTAC+TGG + chr7_2:71467701-71467720 Msa1073830:CDS 35.0%
ATTTACTGGATGAGCTAAAG+AGG + chr7_2:71467715-71467734 Msa1073830:exon 35.0%
AGCTAAATAACCGAAAGCTA+AGG - chr7_2:71467786-71467805 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG + chr7_2:71467832-71467851 Msa1073830:three_prime_UTR 35.0%
!! AATGCATCCATTTTGAAGAC+AGG - chr7_2:71468038-71468057 None:intergenic 35.0%
ATTGGTCAATTGATTGCTTC+TGG - chr7_2:71468130-71468149 None:intergenic 35.0%
TCTCACTAACCAAATTTCTC+TGG + chr7_2:71466730-71466749 Msa1073830:five_prime_UTR 35.0%
! TTCAAGATGGCATTAGTTTC+AGG + chr7_2:71467263-71467282 Msa1073830:exon 35.0%
GAATGTAAAGTGGTGCATTA+GGG - chr7_2:71467307-71467326 None:intergenic 35.0%
TGAAATGGTACCATGATTAC+TGG + chr7_2:71467387-71467406 Msa1073830:CDS 35.0%
GATGATGTAGATGAGTTTGA+CGG + chr7_2:71467443-71467462 Msa1073830:CDS 35.0%
AGAGTTTATCAAGTCTTCTG+AGG + chr7_2:71467544-71467563 Msa1073830:CDS 35.0%
CAAGGAGCAAAGAAATTTAC+TGG + chr7_2:71467701-71467720 Msa1073830:CDS 35.0%
ATTTACTGGATGAGCTAAAG+AGG + chr7_2:71467715-71467734 Msa1073830:exon 35.0%
AGCTAAATAACCGAAAGCTA+AGG - chr7_2:71467786-71467805 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG + chr7_2:71467832-71467851 Msa1073830:three_prime_UTR 35.0%
!! AATGCATCCATTTTGAAGAC+AGG - chr7_2:71468038-71468057 None:intergenic 35.0%
ATTGGTCAATTGATTGCTTC+TGG - chr7_2:71468130-71468149 None:intergenic 35.0%
TGACTCGTTTGAGTCAAGAT+CGG - chr7_2:71466913-71466932 None:intergenic 40.0%
GGAAAAAGTGATAAGGGTGA+AGG + chr7_2:71467237-71467256 Msa1073830:five_prime_UTR 40.0%
! AAGATGGCATTAGTTTCAGG+AGG + chr7_2:71467266-71467285 Msa1073830:exon 40.0%
GGAATGTAAAGTGGTGCATT+AGG - chr7_2:71467308-71467327 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG - chr7_2:71467317-71467336 None:intergenic 40.0%
! CTATGTCGAGGATTTTTCTC+CGG + chr7_2:71467340-71467359 Msa1073830:CDS 40.0%
TGATGCGTTTGATCTTGATG+CGG + chr7_2:71467490-71467509 Msa1073830:CDS 40.0%
! TGACAGAACTCAGAAGTGTA+TGG + chr7_2:71468077-71468096 Msa1073830:three_prime_UTR 40.0%
GCTTCTGGACATGTATTGTA+TGG - chr7_2:71468115-71468134 None:intergenic 40.0%
TGACTCGTTTGAGTCAAGAT+CGG - chr7_2:71466913-71466932 None:intergenic 40.0%
GGAAAAAGTGATAAGGGTGA+AGG + chr7_2:71467237-71467256 Msa1073830:five_prime_UTR 40.0%
! AAGATGGCATTAGTTTCAGG+AGG + chr7_2:71467266-71467285 Msa1073830:exon 40.0%
GGAATGTAAAGTGGTGCATT+AGG - chr7_2:71467308-71467327 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG - chr7_2:71467317-71467336 None:intergenic 40.0%
! CTATGTCGAGGATTTTTCTC+CGG + chr7_2:71467340-71467359 Msa1073830:CDS 40.0%
TGATGCGTTTGATCTTGATG+CGG + chr7_2:71467490-71467509 Msa1073830:CDS 40.0%
! TGACAGAACTCAGAAGTGTA+TGG + chr7_2:71468077-71468096 Msa1073830:three_prime_UTR 40.0%
GCTTCTGGACATGTATTGTA+TGG - chr7_2:71468115-71468134 None:intergenic 40.0%
AGGGTGAAGGTGATTCAAGA+TGG + chr7_2:71467250-71467269 Msa1073830:exon 45.0%
! TCGAGGATTTTTCTCCGGAA+TGG + chr7_2:71467345-71467364 Msa1073830:CDS 45.0%
! AGGATTTTTCTCCGGAATGG+TGG + chr7_2:71467348-71467367 Msa1073830:CDS 45.0%
AGCTGGTGACAACACTGAAA+TGG + chr7_2:71467372-71467391 Msa1073830:CDS 45.0%
! AGCCAGCAACATGATGATGA+AGG + chr7_2:71467413-71467432 Msa1073830:CDS 45.0%
AGCCTTCATCATCATGTTGC+TGG - chr7_2:71467418-71467437 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG - chr7_2:71467491-71467510 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG + chr7_2:71467551-71467570 Msa1073830:CDS 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG + chr7_2:71467683-71467702 Msa1073830:intron 45.0%
AGGGTGAAGGTGATTCAAGA+TGG + chr7_2:71467250-71467269 Msa1073830:exon 45.0%
! TCGAGGATTTTTCTCCGGAA+TGG + chr7_2:71467345-71467364 Msa1073830:CDS 45.0%
! AGGATTTTTCTCCGGAATGG+TGG + chr7_2:71467348-71467367 Msa1073830:CDS 45.0%
AGCTGGTGACAACACTGAAA+TGG + chr7_2:71467372-71467391 Msa1073830:CDS 45.0%
! AGCCAGCAACATGATGATGA+AGG + chr7_2:71467413-71467432 Msa1073830:CDS 45.0%
AGCCTTCATCATCATGTTGC+TGG - chr7_2:71467418-71467437 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG - chr7_2:71467491-71467510 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG + chr7_2:71467551-71467570 Msa1073830:CDS 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG + chr7_2:71467683-71467702 Msa1073830:intron 45.0%
!! GTTAAAATTAAAATTAAAAT+CGG - chr7_2:71466860-71466879 None:intergenic 5.0%
!! GTTAAAATTAAAATTAAAAT+CGG - chr7_2:71466860-71466879 None:intergenic 5.0%
TCGACATAGCGAAATGCAGC+TGG - chr7_2:71467329-71467348 None:intergenic 50.0%
AGCTGCATTTCGCTATGTCG+AGG + chr7_2:71467328-71467347 Msa1073830:CDS 50.0%
GCTGGCTAAGCCAGTAATCA+TGG - chr7_2:71467400-71467419 None:intergenic 50.0%
ACCATAGACGCTGAATGCGA+CGG + chr7_2:71467755-71467774 Msa1073830:three_prime_UTR 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG + chr7_2:71467773-71467792 Msa1073830:three_prime_UTR 50.0%
TCGACATAGCGAAATGCAGC+TGG - chr7_2:71467329-71467348 None:intergenic 50.0%
AGCTGCATTTCGCTATGTCG+AGG + chr7_2:71467328-71467347 Msa1073830:CDS 50.0%
GCTGGCTAAGCCAGTAATCA+TGG - chr7_2:71467400-71467419 None:intergenic 50.0%
ACCATAGACGCTGAATGCGA+CGG + chr7_2:71467755-71467774 Msa1073830:three_prime_UTR 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG + chr7_2:71467773-71467792 Msa1073830:three_prime_UTR 50.0%
ACCGTCGCATTCAGCGTCTA+TGG - chr7_2:71467759-71467778 None:intergenic 55.0%
ACCGTCGCATTCAGCGTCTA+TGG - chr7_2:71467759-71467778 None:intergenic 55.0%
TTCTCCGGAATGGTGGCAGC+TGG + chr7_2:71467355-71467374 Msa1073830:CDS 60.0%
GTCACCAGCTGCCACCATTC+CGG - chr7_2:71467362-71467381 None:intergenic 60.0%
TTCTCCGGAATGGTGGCAGC+TGG + chr7_2:71467355-71467374 Msa1073830:CDS 60.0%
GTCACCAGCTGCCACCATTC+CGG - chr7_2:71467362-71467381 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 71466716 71468165 71466716 ID=Msa1073830;Name=Msa1073830
chr7_2 mRNA 71466716 71468165 71466716 ID=Msa1073830-mRNA-1;Parent=Msa1073830;Name=Msa1073830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=73|1|1|1|0.5|0.33|3|438|115
chr7_2 exon 71466716 71466751 71466716 ID=Msa1073830-mRNA-1:exon:16174;Parent=Msa1073830-mRNA-1
chr7_2 exon 71467232 71467572 71467232 ID=Msa1073830-mRNA-1:exon:16175;Parent=Msa1073830-mRNA-1
chr7_2 exon 71467684 71468165 71467684 ID=Msa1073830-mRNA-1:exon:16176;Parent=Msa1073830-mRNA-1
chr7_2 five_prime_UTR 71466716 71466751 71466716 ID=Msa1073830-mRNA-1:five_prime_utr;Parent=Msa1073830-mRNA-1
chr7_2 five_prime_UTR 71467232 71467268 71467232 ID=Msa1073830-mRNA-1:five_prime_utr;Parent=Msa1073830-mRNA-1
chr7_2 CDS 71467269 71467572 71467269 ID=Msa1073830-mRNA-1:cds;Parent=Msa1073830-mRNA-1
chr7_2 CDS 71467684 71467727 71467684 ID=Msa1073830-mRNA-1:cds;Parent=Msa1073830-mRNA-1
chr7_2 three_prime_UTR 71467728 71468165 71467728 ID=Msa1073830-mRNA-1:three_prime_utr;Parent=Msa1073830-mRNA-1
Gene Sequence

>Msa1073830

ATGGCATTAGTTTCAGGAGGAAGATCAACACTAAACCCTAATGCACCACTTTACATTCCAGCTGCATTTCGCTATGTCGAGGATTTTTCTCCGGAATGGTGGCAGCTGGTGACAACACTGAAATGGTACCATGATTACTGGCTTAGCCAGCAACATGATGATGAAGGCTTTTATGATGATGTAGATGAGTTTGACGGCAATGATGTCGTTGATTTGCTTCCTGATGCGTTTGATCTTGATGCGGATGAAGATCTTTCGATTGAAGCTCAGCTTGAAGAGTTTATCAAGTCTTCTGAGGCACAAGAGGAAAGTTTTGTGGAGCAAGGAGCAAAGAAATTTACTGGATGA

Protein sequence

>Msa1073830

MALVSGGRSTLNPNAPLYIPAAFRYVEDFSPEWWQLVTTLKWYHDYWLSQQHDDEGFYDDVDEFDGNDVVDLLPDAFDLDADEDLSIEAQLEEFIKSSEAQEESFVEQGAKKFTG*