AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1212840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212840 MtrunA17_Chr4g0061951 97.531 81 2 0 20 100 294 374 1.09e-52 168
Msa1212840 MtrunA17_Chr4g0061931 83.562 73 12 0 28 100 128 200 1.67e-38 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212840 AT5G08170.2 79.012 81 17 0 19 99 209 289 4.88e-39 132
Msa1212840 AT5G08170.3 79.012 81 17 0 19 99 209 289 4.88e-39 132
Msa1212840 AT5G08170.5 79.012 81 17 0 19 99 209 289 4.88e-39 132
Msa1212840 AT5G08170.4 79.012 81 17 0 19 99 287 367 1.40e-38 132
Msa1212840 AT5G08170.1 79.012 81 17 0 19 99 293 373 3.00e-38 131

Find 27 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATCATTGCTCCACAATT+TGG 0.199735 8_1:+72817520 Msa1212840:CDS
CTTCTGCCATGGAGAGTTCT+TGG 0.303146 8_1:+72816029 Msa1212840:CDS
GTTGAGAGAATATTTCTAAA+TGG 0.323501 8_1:-72818196 None:intergenic
TTGTGGGAATTGAAGGTTCC+AGG 0.350594 8_1:+72818121 Msa1212840:CDS
TTAAATATTGTAATACTTGT+TGG 0.351571 8_1:+72818345 Msa1212840:three_prime_UTR
GGAGCCAAGCCAAGGCTTCC+TGG 0.371687 8_1:+72817451 Msa1212840:CDS
TCCTAGGTTGTGGGAATTGA+AGG 0.384816 8_1:+72818114 Msa1212840:intron
AGAATGATTGATTAATTGCT+TGG 0.402577 8_1:+72818253 Msa1212840:three_prime_UTR
GGAAGGTCTTAGACAGAATT+CGG 0.404780 8_1:-72817570 None:intergenic
TGGAGAGTTCTTGGCTGTTA+AGG 0.424292 8_1:+72816038 Msa1212840:CDS
AGAGAATATTTCTAAATGGC+TGG 0.455579 8_1:-72818192 None:intergenic
CGTCGAAACACGACTTGCAC+GGG 0.499614 8_1:-72818310 None:intergenic
AGTACCAGGAAGCCTTGGCT+TGG 0.515607 8_1:-72817455 None:intergenic
ACCTGACAAAACAATCTCCC+TGG 0.521534 8_1:-72818139 None:intergenic
AGTCTAGTACCAGGAAGCCT+TGG 0.524063 8_1:-72817460 None:intergenic
TGCAATGTAAAAGTTTACAT+AGG 0.549734 8_1:-72817491 None:intergenic
TTTGTTGATTCCTAGGTTGT+GGG 0.556323 8_1:+72818105 Msa1212840:intron
GTGATAGAAAACTTCTGCCA+TGG 0.557765 8_1:+72816018 Msa1212840:CDS
AAAATACTCACTTCATGATG+AGG 0.559236 8_1:-72817592 None:intergenic
TGTGCGTCTAGGCAATGAAA+AGG 0.561917 8_1:+72815987 None:intergenic
TGTGGGAATTGAAGGTTCCA+GGG 0.582236 8_1:+72818122 Msa1212840:CDS
AAATACTCACTTCATGATGA+GGG 0.594366 8_1:-72817591 None:intergenic
TCGTGTTTCGACGCACATTG+TGG 0.604809 8_1:+72818320 Msa1212840:three_prime_UTR
GAAGCAGCAAGTCTAGTACC+AGG 0.605026 8_1:-72817469 None:intergenic
AGGATGATGGAGCCAAGCCA+AGG 0.607977 8_1:+72817443 Msa1212840:intron
TAACAGCCAAGAACTCTCCA+TGG 0.624393 8_1:-72816035 None:intergenic
ACTCACTTCATGATGAGGGA+AGG 0.632244 8_1:-72817587 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTAATTTATTTTTTT+TGG + chr8_1:72817609-72817628 Msa1212840:intron 0.0%
!! TTGGTATAAAAAATAATTAA+AGG + chr8_1:72816378-72816397 Msa1212840:intron 10.0%
!! TTAGGAAAATAAAAATAAAA+AGG + chr8_1:72816623-72816642 Msa1212840:intron 10.0%
!! ATAAATTAGATTTAGATATT+GGG + chr8_1:72817154-72817173 Msa1212840:intron 10.0%
!!! AGCTAAAGTTTAAAATATAT+TGG + chr8_1:72816312-72816331 Msa1212840:intron 15.0%
!!! TTTTTTCTACTTCATTATTA+TGG + chr8_1:72816343-72816362 Msa1212840:intron 15.0%
!!! TCTACAATAATTTGATAATT+AGG + chr8_1:72816961-72816980 Msa1212840:intron 15.0%
!! CATAAATTAGATTTAGATAT+TGG + chr8_1:72817153-72817172 Msa1212840:intron 15.0%
!!! AACTTTGATATGTATTTTAT+TGG + chr8_1:72818219-72818238 Msa1212840:three_prime_UTR 15.0%
!! TTAAATATTGTAATACTTGT+TGG + chr8_1:72818345-72818364 Msa1212840:three_prime_UTR 15.0%
!!! AGTTTAAAATATATTGGTGT+TGG + chr8_1:72816318-72816337 Msa1212840:intron 20.0%
!!! ATTATGGTTTGATGTTATTT+TGG + chr8_1:72816359-72816378 Msa1212840:intron 20.0%
!!! TAATATCATGTTTTTCTGTT+TGG + chr8_1:72816742-72816761 Msa1212840:intron 20.0%
!! TTTAAATTGAACACTGTAAA+AGG + chr8_1:72817127-72817146 Msa1212840:intron 20.0%
! AAAAATCGTAGTCTAGATAA+GGG - chr8_1:72816153-72816172 None:intergenic 25.0%
! GACACAATTTCATTTCATAT+AGG + chr8_1:72816577-72816596 Msa1212840:intron 25.0%
! AAAAAATTGCTTACAGCATA+GGG - chr8_1:72816706-72816725 None:intergenic 25.0%
! TAAAAAATTGCTTACAGCAT+AGG - chr8_1:72816707-72816726 None:intergenic 25.0%
!! TTTTTCATATAATGAGTTGC+AGG - chr8_1:72817273-72817292 None:intergenic 25.0%
!! GGTGTAATTCTATATTTTTC+AGG + chr8_1:72817423-72817442 Msa1212840:intron 25.0%
!!! TTCTATATTTTTCAGGATGA+TGG + chr8_1:72817430-72817449 Msa1212840:intron 25.0%
! TGCAATGTAAAAGTTTACAT+AGG - chr8_1:72817494-72817513 None:intergenic 25.0%
!! GTAAACTTTTACATTGCAAA+TGG + chr8_1:72817496-72817515 Msa1212840:CDS 25.0%
! ACAATTTGGAGATAAAAAGT+GGG + chr8_1:72817534-72817553 Msa1212840:CDS 25.0%
! TATAATGAAACTACTGGATT+GGG - chr8_1:72817976-72817995 None:intergenic 25.0%
! TAGTACTATAATGAAACTAC+TGG - chr8_1:72817982-72818001 None:intergenic 25.0%
! GTTTATGAACTGTAACATTT+AGG + chr8_1:72818037-72818056 Msa1212840:intron 25.0%
!!! TATAATGTTTGTTGATTCCT+AGG + chr8_1:72818098-72818117 Msa1212840:intron 25.0%
!! GTTGAGAGAATATTTCTAAA+TGG - chr8_1:72818199-72818218 None:intergenic 25.0%
! AGAATGATTGATTAATTGCT+TGG + chr8_1:72818253-72818272 Msa1212840:three_prime_UTR 25.0%
CATTACCTTTCATTTCTAAG+AGG + chr8_1:72816087-72816106 Msa1212840:intron 30.0%
! ATTAGCCTCTTAGAAATGAA+AGG - chr8_1:72816095-72816114 None:intergenic 30.0%
! TCATTTCTAAGAGGCTAATT+AGG + chr8_1:72816096-72816115 Msa1212840:intron 30.0%
AAAATCGTAGTCTAGATAAG+GGG - chr8_1:72816152-72816171 None:intergenic 30.0%
CAAAAATCGTAGTCTAGATA+AGG - chr8_1:72816154-72816173 None:intergenic 30.0%
! CTTATCTAGACTACGATTTT+TGG + chr8_1:72816152-72816171 Msa1212840:intron 30.0%
TGATGATATGTCTTCAATTC+TGG + chr8_1:72816182-72816201 Msa1212840:intron 30.0%
ATAGTAGTATGAAGATACAC+TGG + chr8_1:72816456-72816475 Msa1212840:intron 30.0%
AAATGAAATTGTGTCCAAAC+AGG - chr8_1:72816572-72816591 None:intergenic 30.0%
!!! TGCTGTAAGCAATTTTTTAG+AGG + chr8_1:72816708-72816727 Msa1212840:intron 30.0%
ATTGTAGAGATGCAATCATA+GGG - chr8_1:72816949-72816968 None:intergenic 30.0%
TATTGTAGAGATGCAATCAT+AGG - chr8_1:72816950-72816969 None:intergenic 30.0%
TATCCTATTGGAAACAAAAC+TGG - chr8_1:72817011-72817030 None:intergenic 30.0%
!!! AGTTTTGTTTCCAATAGGAT+AGG + chr8_1:72817010-72817029 Msa1212840:intron 30.0%
GATATTACATCCTATCCTAT+TGG - chr8_1:72817023-72817042 None:intergenic 30.0%
ATCTGAGTAGTAAATGCAAT+AGG - chr8_1:72817072-72817091 None:intergenic 30.0%
TATTCATTCTTAACCATACC+AGG - chr8_1:72817330-72817349 None:intergenic 30.0%
TACACAATATAAACCTCTAC+TGG + chr8_1:72817393-72817412 Msa1212840:intron 30.0%
!! CACTTTTTATCTCCAAATTG+TGG - chr8_1:72817535-72817554 None:intergenic 30.0%
CACAATTTGGAGATAAAAAG+TGG + chr8_1:72817533-72817552 Msa1212840:CDS 30.0%
AAATACTCACTTCATGATGA+GGG - chr8_1:72817594-72817613 None:intergenic 30.0%
AAAATACTCACTTCATGATG+AGG - chr8_1:72817595-72817614 None:intergenic 30.0%
!! CTTATTCATGATGATTTGGT+TGG - chr8_1:72817909-72817928 None:intergenic 30.0%
ATCGCTTATTCATGATGATT+TGG - chr8_1:72817913-72817932 None:intergenic 30.0%
CTATAATGAAACTACTGGAT+TGG - chr8_1:72817977-72817996 None:intergenic 30.0%
!!! GTATCACTTTTGAGTTGTTT+TGG + chr8_1:72818059-72818078 Msa1212840:intron 30.0%
! AGAGAATATTTCTAAATGGC+TGG - chr8_1:72818195-72818214 None:intergenic 30.0%
! GATTTTCAAATGCAACTAGT+TGG + chr8_1:72818284-72818303 Msa1212840:three_prime_UTR 30.0%
TAACGTTTGAACCCATTTGT+AGG + chr8_1:72816061-72816080 Msa1212840:intron 35.0%
! AGGTTGTCATTCAGAGTATT+TGG + chr8_1:72816398-72816417 Msa1212840:intron 35.0%
AAATAGCTTCAAACACAGGT+AGG - chr8_1:72816491-72816510 None:intergenic 35.0%
TGTGTTATCCTAATCCTGTT+TGG + chr8_1:72816555-72816574 Msa1212840:intron 35.0%
AATTGTGTCCAAACAGGATT+AGG - chr8_1:72816566-72816585 None:intergenic 35.0%
! TGCTCACTGGTTAGTTATTT+AGG + chr8_1:72816605-72816624 Msa1212840:intron 35.0%
! GATGCTAAATTTTGCAACTG+CGG + chr8_1:72816645-72816664 Msa1212840:intron 35.0%
TATTAGCCAAATTGCTCACT+TGG + chr8_1:72816779-72816798 Msa1212840:intron 35.0%
!!! AAGCCAGTTTTGTTTCCAAT+AGG + chr8_1:72817005-72817024 Msa1212840:intron 35.0%
TAGCAAGATTGATTGATACC+TGG + chr8_1:72817309-72817328 Msa1212840:intron 35.0%
AGATTGATTGATACCTGGTA+TGG + chr8_1:72817314-72817333 Msa1212840:intron 35.0%
TTTCCAAGTGATGAAGTTTG+TGG + chr8_1:72817769-72817788 Msa1212840:intron 35.0%
!! TTTGTTGATTCCTAGGTTGT+GGG + chr8_1:72818105-72818124 Msa1212840:intron 35.0%
GTGATAGAAAACTTCTGCCA+TGG + chr8_1:72816018-72816037 Msa1212840:CDS 40.0%
AGGTAATGCTGCCTACAAAT+GGG - chr8_1:72816075-72816094 None:intergenic 40.0%
AAGGTAATGCTGCCTACAAA+TGG - chr8_1:72816076-72816095 None:intergenic 40.0%
! TCACCCTGCTATAGTGTTTT+TGG + chr8_1:72816252-72816271 Msa1212840:intron 40.0%
! CACCCTGCTATAGTGTTTTT+GGG + chr8_1:72816253-72816272 Msa1212840:intron 40.0%
AGCCCAAAAACACTATAGCA+GGG - chr8_1:72816258-72816277 None:intergenic 40.0%
TAAACTTTAGCTGCCAATCC+TGG - chr8_1:72816304-72816323 None:intergenic 40.0%
CAGCAAATAGCTTCAAACAC+AGG - chr8_1:72816495-72816514 None:intergenic 40.0%
! GTGTTTGAAGCTATTTGCTG+AGG + chr8_1:72816495-72816514 Msa1212840:intron 40.0%
AGGTAACCAAGTGAGCAATT+TGG - chr8_1:72816788-72816807 None:intergenic 40.0%
TACCTATGCACAGAACTACT+CGG + chr8_1:72816803-72816822 Msa1212840:intron 40.0%
GATATTGGGCATGCAATCTT+TGG + chr8_1:72817168-72817187 Msa1212840:intron 40.0%
TGCAGGATAGTCTCATCATT+AGG - chr8_1:72817256-72817275 None:intergenic 40.0%
TAAACCTCTACTGGATGTTC+TGG + chr8_1:72817402-72817421 Msa1212840:intron 40.0%
GCTATCATTGCTCCACAATT+TGG + chr8_1:72817520-72817539 Msa1212840:CDS 40.0%
GGAAGGTCTTAGACAGAATT+CGG - chr8_1:72817573-72817592 None:intergenic 40.0%
CACTAACTACTGCAGTTATG+TGG + chr8_1:72817718-72817737 Msa1212840:intron 40.0%
TAGTTTGAGCCACAGATCTT+CGG - chr8_1:72817795-72817814 None:intergenic 40.0%
! GATTTGGTTGGATGAGAACA+TGG - chr8_1:72817897-72817916 None:intergenic 40.0%
! TTGGGACATTTGCGATTTTG+TGG - chr8_1:72817958-72817977 None:intergenic 40.0%
!! GTTTGTTGATTCCTAGGTTG+TGG + chr8_1:72818104-72818123 Msa1212840:intron 40.0%
TAACAGCCAAGAACTCTCCA+TGG - chr8_1:72816038-72816057 None:intergenic 45.0%
TGGAGAGTTCTTGGCTGTTA+AGG + chr8_1:72816038-72816057 Msa1212840:CDS 45.0%
CAGCCCAAAAACACTATAGC+AGG - chr8_1:72816259-72816278 None:intergenic 45.0%
!!! CTGCTATAGTGTTTTTGGGC+TGG + chr8_1:72816257-72816276 Msa1212840:intron 45.0%
CACATTTATCCAGCCAGGAT+TGG + chr8_1:72816288-72816307 Msa1212840:intron 45.0%
GCTTCAAACACAGGTAGGAA+TGG - chr8_1:72816486-72816505 None:intergenic 45.0%
! CATATAGGCGATTTGCTCAC+TGG + chr8_1:72816592-72816611 Msa1212840:intron 45.0%
! NTCCGAGTAGTTCTGTGCAT+AGG - chr8_1:72816808-72816827 None:intergenic 45.0%
GCAATCATAGGGTTACCACT+GGG - chr8_1:72816938-72816957 None:intergenic 45.0%
TGCAATCATAGGGTTACCAC+TGG - chr8_1:72816939-72816958 None:intergenic 45.0%
GCTGAGAGCAAAACAATGAG+AGG - chr8_1:72817104-72817123 None:intergenic 45.0%
!! GCATGCAATCTTTGGAATGC+TGG + chr8_1:72817176-72817195 Msa1212840:intron 45.0%
GAATGCTGGTAGTTAGCAGT+CGG + chr8_1:72817190-72817209 Msa1212840:intron 45.0%
TACACCAGAACATCCAGTAG+AGG - chr8_1:72817409-72817428 None:intergenic 45.0%
ACTCACTTCATGATGAGGGA+AGG - chr8_1:72817590-72817609 None:intergenic 45.0%
TCCTAGGTTGTGGGAATTGA+AGG + chr8_1:72818114-72818133 Msa1212840:intron 45.0%
ACCTTCAATTCCCACAACCT+AGG - chr8_1:72818118-72818137 None:intergenic 45.0%
TTGTGGGAATTGAAGGTTCC+AGG + chr8_1:72818121-72818140 Msa1212840:CDS 45.0%
TGTGGGAATTGAAGGTTCCA+GGG + chr8_1:72818122-72818141 Msa1212840:CDS 45.0%
!! TCCAGGGAGATTGTTTTGTC+AGG + chr8_1:72818138-72818157 Msa1212840:CDS 45.0%
ACCTGACAAAACAATCTCCC+TGG - chr8_1:72818142-72818161 None:intergenic 45.0%
!! AGGGAGATTGTTTTGTCAGG+TGG + chr8_1:72818141-72818160 Msa1212840:CDS 45.0%
CTTCTGCCATGGAGAGTTCT+TGG + chr8_1:72816029-72816048 Msa1212840:CDS 50.0%
! TAAGGGGAGCTTTGACCCAA+CGG - chr8_1:72816136-72816155 None:intergenic 50.0%
CTCTGCACATTTATCCAGCC+AGG + chr8_1:72816283-72816302 Msa1212840:intron 50.0%
AGTCTAGTACCAGGAAGCCT+TGG - chr8_1:72817463-72817482 None:intergenic 50.0%
GAAGCAGCAAGTCTAGTACC+AGG - chr8_1:72817472-72817491 None:intergenic 50.0%
CGGCCACAAACTTCATCACT+TGG - chr8_1:72817775-72817794 None:intergenic 50.0%
AGTTTGTGGCCGAAGATCTG+TGG + chr8_1:72817783-72817802 Msa1212840:intron 50.0%
TCGTGTTTCGACGCACATTG+TGG + chr8_1:72818320-72818339 Msa1212840:three_prime_UTR 50.0%
GCAGCAACACACCAACCGTT+GGG + chr8_1:72816118-72816137 Msa1212840:intron 55.0%
CTTTAGCTGCCAATCCTGGC+TGG - chr8_1:72816300-72816319 None:intergenic 55.0%
!! AGGATGATGGAGCCAAGCCA+AGG + chr8_1:72817443-72817462 Msa1212840:intron 55.0%
AGTACCAGGAAGCCTTGGCT+TGG - chr8_1:72817458-72817477 None:intergenic 55.0%
CGTCGAAACACGACTTGCAC+GGG - chr8_1:72818313-72818332 None:intergenic 55.0%
GCGTCGAAACACGACTTGCA+CGG - chr8_1:72818314-72818333 None:intergenic 55.0%
GGCAGCAACACACCAACCGT+TGG + chr8_1:72816117-72816136 Msa1212840:intron 60.0%
! GGGAGCTTTGACCCAACGGT+TGG - chr8_1:72816132-72816151 None:intergenic 60.0%
GGAGCCAAGCCAAGGCTTCC+TGG + chr8_1:72817451-72817470 Msa1212840:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 72816000 72818455 72816000 ID=Msa1212840;Name=Msa1212840
chr8_1 mRNA 72816000 72818455 72816000 ID=Msa1212840-mRNA-1;Parent=Msa1212840;Name=Msa1212840-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.5|0.33|0.66|1|0.66|3|252|100
chr8_1 exon 72816000 72816059 72816000 ID=Msa1212840-mRNA-1:exon:24620;Parent=Msa1212840-mRNA-1
chr8_1 exon 72817445 72817603 72817445 ID=Msa1212840-mRNA-1:exon:24621;Parent=Msa1212840-mRNA-1
chr8_1 exon 72818120 72818455 72818120 ID=Msa1212840-mRNA-1:exon:24622;Parent=Msa1212840-mRNA-1
chr8_1 CDS 72816000 72816059 72816000 ID=Msa1212840-mRNA-1:cds;Parent=Msa1212840-mRNA-1
chr8_1 CDS 72817445 72817603 72817445 ID=Msa1212840-mRNA-1:cds;Parent=Msa1212840-mRNA-1
chr8_1 CDS 72818120 72818203 72818120 ID=Msa1212840-mRNA-1:cds;Parent=Msa1212840-mRNA-1
chr8_1 three_prime_UTR 72818204 72818455 72818204 ID=Msa1212840-mRNA-1:three_prime_utr;Parent=Msa1212840-mRNA-1
Gene Sequence

>Msa1212840

AATGAAAAGGACAATCTAGTGATAGAAAACTTCTGCCATGGAGAGTTCTTGGCTGTTAAGGATGATGGAGCCAAGCCAAGGCTTCCTGGTACTAGACTTGCTGCTTCCTATGTAAACTTTTACATTGCAAATGGTGCTATCATTGCTCCACAATTTGGAGATAAAAAGTGGGATGATGAAGCTATCCGAATTCTGTCTAAGACCTTCCCTCATCATGAAGTTGTGGGAATTGAAGGTTCCAGGGAGATTGTTTTGTCAGGTGGAAATATACATTGCATCACTCAGCAACAACCAGCCATTTAG

Protein sequence

>Msa1212840

NEKDNLVIENFCHGEFLAVKDDGAKPRLPGTRLAASYVNFYIANGAIIAPQFGDKKWDDEAIRILSKTFPHHEVVGIEGSREIVLSGGNIHCITQQQPAI*