AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732400 MtrunA17_Chr5g0437601 99.683 315 1 0 6 320 1 315 0.0 660
Msa0732400 MtrunA17_Chr3g0114101 72.586 321 79 5 9 320 1 321 8.28e-164 463
Msa0732400 MtrunA17_Chr3g0113671 51.695 236 114 0 85 320 103 338 1.25e-88 272
Msa0732400 MtrunA17_Chr4g0006991 50.213 235 117 0 86 320 84 318 8.18e-87 267
Msa0732400 MtrunA17_Chr7g0242701 41.139 316 176 4 15 320 3 318 1.16e-86 266
Msa0732400 MtrunA17_Chr4g0005481 53.191 235 107 2 84 318 104 335 2.74e-86 266
Msa0732400 MtrunA17_Chr7g0243301 47.547 265 131 4 58 320 39 297 1.21e-85 263
Msa0732400 MtrunA17_Chr4g0005491 50.638 235 113 2 88 320 62 295 4.90e-85 261
Msa0732400 MtrunA17_Chr8g0360521 50.000 236 114 2 83 318 66 297 1.97e-84 260
Msa0732400 MtrunA17_Chr5g0437291 48.739 238 119 1 86 320 163 400 2.48e-80 253
Msa0732400 MtrunA17_Chr1g0210771 41.176 238 122 7 88 320 59 283 2.12e-54 182
Msa0732400 MtrunA17_Chr1g0171171 34.602 289 170 7 32 320 8 277 5.45e-54 181
Msa0732400 MtrunA17_Chr2g0287901 39.256 242 134 6 86 318 95 332 5.71e-54 182
Msa0732400 MtrunA17_Chr3g0097891 36.929 241 139 6 80 318 23 252 2.84e-53 178
Msa0732400 MtrunA17_Chr4g0041011 32.624 282 173 6 37 318 4 268 6.83e-52 175
Msa0732400 MtrunA17_Chr2g0325481 36.364 231 138 4 88 318 50 271 8.64e-51 172
Msa0732400 MtrunA17_Chr5g0430371 36.471 255 143 6 69 318 40 280 4.66e-49 168
Msa0732400 MtrunA17_Chr5g0413851 40.506 237 126 8 84 318 38 261 5.99e-49 167
Msa0732400 MtrunA17_Chr7g0259711 31.722 331 195 11 7 318 8 326 1.08e-48 169
Msa0732400 MtrunA17_Chr2g0314801 35.950 242 137 6 82 318 27 255 8.75e-48 164
Msa0732400 MtrunA17_Chr3g0105301 35.391 243 139 5 81 318 36 265 5.90e-47 162
Msa0732400 MtrunA17_Chr2g0283581 34.146 287 156 7 32 318 9 262 8.21e-47 161
Msa0732400 MtrunA17_Chr3g0085081 35.021 237 137 6 86 318 29 252 3.15e-46 159
Msa0732400 MtrunA17_Chr5g0413271 35.714 238 147 4 82 318 139 371 4.86e-46 162
Msa0732400 MtrunA17_Chr7g0269991 36.134 238 141 4 88 318 137 370 1.26e-45 161
Msa0732400 MtrunA17_Chr3g0085071 34.188 234 143 5 86 318 44 267 2.91e-45 157
Msa0732400 MtrunA17_Chr4g0045941 31.557 244 152 5 80 320 25 256 4.08e-45 157
Msa0732400 MtrunA17_Chr3g0091031 34.599 237 138 6 86 318 29 252 6.08e-45 156
Msa0732400 MtrunA17_Chr4g0071391 36.402 239 140 5 88 318 109 343 7.04e-44 156
Msa0732400 MtrunA17_Chr2g0314791 36.364 242 137 8 85 318 26 258 9.43e-44 153
Msa0732400 MtrunA17_Chr1g0209921 33.898 236 144 6 86 319 42 267 2.69e-43 149
Msa0732400 MtrunA17_Chr1g0175671 35.124 242 139 5 88 318 170 404 2.72e-43 155
Msa0732400 MtrunA17_Chr1g0213281 34.444 270 160 7 61 318 145 409 1.17e-41 151
Msa0732400 MtrunA17_Chr4g0045951 31.356 236 153 4 83 318 40 266 2.95e-41 147
Msa0732400 MtrunA17_Chr7g0258851 35.102 245 135 6 88 318 248 482 7.82e-41 150
Msa0732400 MtrunA17_Chr4g0068111 30.627 271 173 5 61 320 86 352 1.07e-39 145
Msa0732400 MtrunA17_Chr8g0384151 27.414 321 207 7 10 318 19 325 8.34e-39 142
Msa0732400 MtrunA17_Chr1g0178081 37.912 182 103 3 86 258 53 233 2.74e-37 136
Msa0732400 MtrunA17_Chr3g0126021 29.675 246 160 4 84 318 125 368 4.50e-36 135
Msa0732400 MtrunA17_Chr8g0368461 30.620 258 154 5 81 318 171 423 5.13e-35 133
Msa0732400 MtrunA17_Chr1g0188191 39.130 138 81 3 48 184 195 330 1.37e-28 115
Msa0732400 MtrunA17_Chr6g0456681 30.864 243 154 7 86 318 62 300 6.39e-28 112
Msa0732400 MtrunA17_Chr6g0476041 37.778 135 80 3 86 220 165 295 1.13e-27 112
Msa0732400 MtrunA17_Chr6g0456691 26.814 317 194 10 21 318 14 311 2.62e-27 110
Msa0732400 MtrunA17_Chr7g0221271 40.000 130 77 1 86 215 77 205 6.97e-27 105
Msa0732400 MtrunA17_Chr2g0283851 29.167 264 165 10 69 318 36 291 1.47e-26 108
Msa0732400 MtrunA17_Chr5g0414061 28.992 238 157 7 88 318 94 326 9.94e-26 106
Msa0732400 MtrunA17_Chr8g0347321 26.891 238 162 6 88 318 95 327 9.99e-26 106
Msa0732400 MtrunA17_Chr7g0221291 45.361 97 52 1 84 180 75 170 3.30e-25 99.8
Msa0732400 MtrunA17_Chr5g0414091 30.120 249 148 8 85 318 82 319 9.26e-25 103
Msa0732400 MtrunA17_Chr6g0451321 26.295 251 171 7 78 318 106 352 9.57e-25 103
Msa0732400 MtrunA17_Chr7g0226991 38.060 134 80 2 85 218 54 184 1.54e-24 102
Msa0732400 MtrunA17_Chr8g0391481 38.060 134 80 2 85 218 24 154 1.83e-24 102
Msa0732400 MtrunA17_Chr5g0414051 29.348 184 109 5 85 260 72 242 4.22e-23 99.0
Msa0732400 MtrunA17_Chr5g0414041 30.000 240 154 6 88 318 87 321 7.86e-23 98.2
Msa0732400 MtrunA17_Chr3g0140371 29.268 246 151 9 85 318 15 249 1.11e-21 94.4
Msa0732400 MtrunA17_Chr8g0387921 25.738 237 167 6 88 318 74 307 9.66e-20 89.4
Msa0732400 MtrunA17_Chr8g0384421 26.587 252 162 9 86 318 131 378 8.71e-18 83.6
Msa0732400 MtrunA17_Chr4g0049421 25.000 248 158 9 88 318 48 284 1.94e-16 79.3
Msa0732400 MtrunA17_Chr2g0287931 36.134 119 66 4 148 257 1 118 7.33e-16 73.2
Msa0732400 MtrunA17_Chr6g0455901 24.900 249 170 8 85 318 68 314 1.12e-14 74.3
Msa0732400 MtrunA17_Chr4g0070341 26.119 268 143 12 86 318 130 377 7.81e-13 68.9
Msa0732400 MtrunA17_Chr2g0287911 43.750 64 28 2 81 144 54 109 6.16e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732400 AT2G40320.1 77.592 299 65 1 22 320 17 313 1.35e-174 490
Msa0732400 AT3G11030.1 59.701 335 106 3 13 320 6 338 2.41e-143 412
Msa0732400 AT3G55990.1 55.319 235 105 0 86 320 138 372 8.42e-91 279
Msa0732400 AT2G40150.1 52.846 246 113 1 78 320 58 303 9.40e-89 272
Msa0732400 AT5G01620.2 52.066 242 112 2 78 318 100 338 1.71e-88 272
Msa0732400 AT5G01620.1 52.066 242 112 2 78 318 100 338 1.71e-88 272
Msa0732400 AT5G01620.3 52.066 242 112 2 78 318 108 346 2.90e-88 272
Msa0732400 AT5G01360.2 48.936 235 120 0 86 320 34 268 3.39e-84 259
Msa0732400 AT5G01360.1 48.936 235 120 0 86 320 90 324 1.49e-83 259
Msa0732400 AT1G73140.1 42.642 265 136 2 54 318 45 293 2.07e-82 255
Msa0732400 AT2G38320.1 45.769 260 132 4 66 320 37 292 1.61e-77 243
Msa0732400 AT2G40160.3 39.799 299 166 5 28 318 18 310 5.94e-76 236
Msa0732400 AT2G40160.1 39.799 299 166 5 28 318 18 310 1.46e-74 236
Msa0732400 AT2G40160.2 39.799 299 166 5 28 318 18 310 1.71e-74 236
Msa0732400 AT2G38320.2 49.524 210 97 4 116 320 4 209 2.16e-66 211
Msa0732400 AT5G19160.2 35.275 309 181 8 25 318 30 334 5.74e-56 185
Msa0732400 AT5G19160.1 34.951 309 182 8 25 318 30 334 9.76e-55 185
Msa0732400 AT1G78710.1 37.395 238 136 5 83 318 33 259 3.64e-53 178
Msa0732400 AT3G62390.2 38.314 261 140 8 73 318 117 371 1.81e-52 178
Msa0732400 AT3G62390.1 38.314 261 140 8 73 318 117 371 9.45e-52 177
Msa0732400 AT3G06080.1 35.743 249 146 5 81 318 93 338 2.21e-51 173
Msa0732400 AT2G30900.1 36.434 258 142 8 73 320 23 268 6.44e-51 172
Msa0732400 AT3G14850.2 38.272 243 131 5 83 320 29 257 6.91e-51 172
Msa0732400 AT5G20590.2 37.849 251 143 6 77 318 130 376 1.33e-50 172
Msa0732400 AT3G06080.2 35.743 249 146 5 81 318 93 338 6.80e-50 172
Msa0732400 AT5G20590.1 37.849 251 143 6 77 318 130 376 1.74e-49 172
Msa0732400 AT3G54260.1 38.865 229 132 5 88 316 51 271 1.76e-48 167
Msa0732400 AT2G30900.2 35.271 258 145 8 73 320 23 268 3.77e-48 163
Msa0732400 AT2G42570.1 33.462 260 160 5 59 318 22 268 6.20e-48 165
Msa0732400 AT3G12060.1 35.206 267 149 6 66 318 174 430 5.64e-47 166
Msa0732400 AT2G31110.2 35.622 233 137 5 88 318 45 266 7.02e-47 162
Msa0732400 AT1G29050.1 33.051 236 150 3 83 318 56 283 1.10e-46 162
Msa0732400 AT2G34070.1 33.750 240 144 5 83 318 60 288 3.37e-46 160
Msa0732400 AT2G31110.3 35.088 228 135 5 88 313 45 261 9.50e-46 156
Msa0732400 AT5G06700.1 35.772 246 136 7 86 318 253 489 2.07e-44 160
Msa0732400 AT1G78710.2 40.341 176 97 3 83 258 26 193 5.14e-44 153
Msa0732400 AT5G58600.2 33.473 239 140 5 88 320 66 291 6.99e-44 152
Msa0732400 AT2G30010.1 34.831 267 149 7 59 318 33 281 7.04e-44 155
Msa0732400 AT2G30010.2 34.831 267 149 7 59 318 76 324 1.63e-43 155
Msa0732400 AT5G58600.1 33.333 237 139 5 88 318 66 289 3.74e-42 150
Msa0732400 AT5G06230.3 34.524 252 134 7 88 318 62 303 6.27e-42 147
Msa0732400 AT1G48880.1 30.719 306 179 9 28 318 63 350 7.93e-42 150
Msa0732400 AT1G60790.1 33.333 243 148 5 86 318 188 426 1.25e-41 152
Msa0732400 AT5G06230.2 34.524 252 134 7 88 318 21 262 3.55e-41 147
Msa0732400 AT5G49340.1 33.463 257 157 6 73 318 83 336 5.53e-41 149
Msa0732400 AT5G06230.1 34.524 252 134 7 88 318 62 303 7.46e-41 147
Msa0732400 AT3G11570.1 33.184 223 137 3 105 318 98 317 7.37e-35 131
Msa0732400 AT1G70230.1 31.020 245 148 7 83 318 74 306 2.56e-30 119
Msa0732400 AT3G28150.1 28.065 310 180 11 23 318 25 305 1.06e-28 114
Msa0732400 AT5G15900.1 28.623 276 180 9 55 318 32 302 6.68e-28 112
Msa0732400 AT5G15900.2 28.623 276 180 9 55 318 51 321 6.83e-28 112
Msa0732400 AT5G15890.1 30.579 242 149 7 85 318 184 414 1.17e-27 113
Msa0732400 AT1G01430.1 39.098 133 78 3 88 219 99 229 3.23e-25 105
Msa0732400 AT5G51640.1 46.875 96 50 1 85 180 135 229 6.76e-25 105
Msa0732400 AT3G02440.1 27.311 238 161 6 88 318 127 359 6.76e-25 104
Msa0732400 AT3G02440.2 27.311 238 161 6 88 318 135 367 8.54e-25 104
Msa0732400 AT4G01080.1 30.380 237 150 9 88 318 91 318 2.32e-24 102
Msa0732400 AT4G25360.1 43.000 100 56 1 81 180 168 266 3.95e-24 102
Msa0732400 AT4G25360.2 43.000 100 56 1 81 180 168 266 3.95e-24 102
Msa0732400 AT4G23790.1 25.566 309 200 12 25 318 21 314 4.55e-24 102
Msa0732400 AT5G64470.4 27.801 241 161 8 88 318 55 292 4.84e-21 92.0
Msa0732400 AT5G64470.1 27.801 241 161 8 88 318 55 292 5.45e-21 92.0
Msa0732400 AT3G14850.1 33.533 167 93 4 159 320 1 154 1.11e-20 89.7
Msa0732400 AT5G64470.3 27.801 241 161 8 88 318 55 292 1.90e-20 91.3
Msa0732400 AT5G64470.2 27.801 241 161 8 88 318 55 292 2.19e-20 91.3
Msa0732400 AT2G34070.2 29.878 164 101 4 159 318 1 154 1.90e-19 86.3
Msa0732400 AT4G11090.1 27.500 200 130 6 28 218 16 209 3.08e-19 88.2
Msa0732400 AT2G14530.1 25.410 244 167 6 88 318 62 303 4.62e-18 84.7
Msa0732400 AT2G37720.1 27.174 276 161 13 71 320 129 390 5.74e-18 84.7
Msa0732400 AT5G64020.1 26.336 262 150 12 88 320 57 304 1.39e-16 80.1
Msa0732400 AT5G20680.6 24.528 265 155 11 84 318 114 363 1.86e-15 77.0
Msa0732400 AT5G20680.2 24.528 265 155 11 84 318 190 439 1.97e-15 77.4
Msa0732400 AT5G20680.4 24.528 265 155 11 84 318 208 457 2.23e-15 77.0
Msa0732400 AT5G20680.1 24.528 265 155 11 84 318 208 457 2.23e-15 77.0
Msa0732400 AT5G20680.3 24.528 265 155 11 84 318 208 457 2.23e-15 77.0
Msa0732400 AT5G20680.5 24.528 265 155 11 84 318 208 457 2.23e-15 77.0

Find 78 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAAGGTGTTGACATTT+TGG 0.194283 5_1:+44800544 Msa0732400:CDS
TGTCGGGTCTCCCATGTTCT+TGG 0.253030 5_1:-44799553 None:intergenic
ATGCAACAATTGAGTTCTAT+TGG 0.254941 5_1:+44800423 Msa0732400:CDS
TCAATAAGCATGGTAGAAAT+TGG 0.262180 5_1:+44800525 Msa0732400:CDS
ATCAAAGGTTTCCATGGATT+TGG 0.299044 5_1:-44799883 None:intergenic
GAAGATTTCATGTGCATCTT+TGG 0.320970 5_1:+44799104 Msa0732400:CDS
TATTCAATGGAAGGTGGGTT+TGG 0.326490 5_1:+44799465 Msa0732400:CDS
TGGTATTCAACACTTATCTT+TGG 0.343420 5_1:+44800564 Msa0732400:CDS
ATTCAATGGAAGGTGGGTTT+GGG 0.383497 5_1:+44799466 Msa0732400:CDS
CGGACAATGCTGTTGTTCAT+AGG 0.387335 5_1:+44800471 Msa0732400:CDS
ACGACCTTTGCTTCTTAGAA+TGG 0.393423 5_1:-44799021 None:intergenic
CCAATGTTGGTAATCTTTGT+CGG 0.403476 5_1:-44799570 None:intergenic
CTATGAAAAGTATGCTTAGA+TGG 0.410597 5_1:+44800799 Msa0732400:CDS
TCTGTCTTCCCCACCGCAAA+TGG 0.411531 5_1:-44799428 None:intergenic
TGCAACAATTGAGTTCTATT+GGG 0.419007 5_1:+44800424 Msa0732400:CDS
TATTCAACACTTATCTTTGG+TGG 0.424745 5_1:+44800567 Msa0732400:CDS
TCCATGGATTTGGCATCCTC+AGG 0.427436 5_1:-44799873 None:intergenic
ACTTATCTTTGGTGGATGAC+TGG 0.435500 5_1:+44800575 Msa0732400:CDS
ATAGTGAGTTGAGTTGGATT+TGG 0.450733 5_1:+44798957 None:intergenic
CATCCTCAGGAATGAGTTGA+TGG 0.450737 5_1:-44799860 None:intergenic
CAATGTTGGTAATCTTTGTC+GGG 0.457780 5_1:-44799569 None:intergenic
GCTAGAAGGGTGAAAAGGTA+AGG 0.463485 5_1:-44799050 None:intergenic
TGAAAGCTAGAAGGGTGAAA+AGG 0.463624 5_1:-44799055 None:intergenic
TGGAAGGTGGGTTTGGGACG+AGG 0.477341 5_1:+44799472 Msa0732400:CDS
TCTGATAGAATTGTAAGAAA+AGG 0.484458 5_1:+44800497 Msa0732400:CDS
ACGACCACTGTACGAGGAAT+CGG 0.484764 5_1:+44799499 Msa0732400:CDS
TAGTGAATCAAAGGTTTCCA+TGG 0.492850 5_1:-44799889 None:intergenic
GGAAAGAGGATGATGTTTGT+TGG 0.493089 5_1:+44799792 Msa0732400:CDS
GCAGATCAAGGTGCCATTTG+CGG 0.494304 5_1:+44799415 Msa0732400:CDS
GAAGGGTGTGATATATTCAA+TGG 0.494550 5_1:+44799452 Msa0732400:CDS
GTGATATATTCAATGGAAGG+TGG 0.495098 5_1:+44799459 Msa0732400:CDS
TCTTTGGTGGATGACTGGCT+TGG 0.496770 5_1:+44800580 Msa0732400:CDS
GAGTTTGATTCTAGAAGAAA+AGG 0.501756 5_1:-44800449 None:intergenic
TGTTCTTGGCAAGTCAATTG+AGG 0.502642 5_1:-44799539 None:intergenic
AACTGAGGATGCTTATCGTA+TGG 0.502726 5_1:+44800776 Msa0732400:CDS
CAATTGACTTGCCAAGAACA+TGG 0.509598 5_1:+44799542 Msa0732400:CDS
GTCAATTGAGGTTGTATGTA+CGG 0.512274 5_1:-44799527 None:intergenic
GACAAATTCTTCTCCACACC+TGG 0.515054 5_1:+44799155 Msa0732400:CDS
ACTAGCATGTCACCTTCTCA+TGG 0.524451 5_1:+44800864 Msa0732400:CDS
GAAAACAGTTAGTGAATCAA+AGG 0.528871 5_1:-44799898 None:intergenic
TGATATATTCAATGGAAGGT+GGG 0.529302 5_1:+44799460 Msa0732400:CDS
CTAGAAGGGTGAAAAGGTAA+GGG 0.530481 5_1:-44799049 None:intergenic
AGAATGGAAGAAGGTGAGAA+TGG 0.531203 5_1:-44799005 None:intergenic
GCGGTGGGGAAGACAGAGGA+AGG 0.532473 5_1:+44799434 Msa0732400:CDS
TTGCTTCTTAGAATGGAAGA+AGG 0.542128 5_1:-44799014 None:intergenic
TCCTGAGGATGCCAAATCCA+TGG 0.545590 5_1:+44799872 Msa0732400:CDS
GAGGGTGAAGAAGCAGATCA+AGG 0.550519 5_1:+44799403 Msa0732400:CDS
TATACTCACTTTCCATGAGA+AGG 0.556322 5_1:-44800876 None:intergenic
ATCAAGGTGCCATTTGCGGT+GGG 0.557278 5_1:+44799419 Msa0732400:CDS
GGTGTGATATATTCAATGGA+AGG 0.558509 5_1:+44799456 Msa0732400:CDS
CACTCCGATTCCTCGTACAG+TGG 0.561364 5_1:-44799503 None:intergenic
TAGAAGGGTGAAAAGGTAAG+GGG 0.565912 5_1:-44799048 None:intergenic
GATCAAGGTGCCATTTGCGG+TGG 0.568008 5_1:+44799418 Msa0732400:CDS
CCGACAAAGATTACCAACAT+TGG 0.570402 5_1:+44799570 Msa0732400:CDS
AACAATGGACTCACCAGGTG+TGG 0.570501 5_1:-44799168 None:intergenic
GTGTGGAGAAGAATTTGTCT+TGG 0.571094 5_1:-44799151 None:intergenic
TTGAAACACTACGTGGAAAG+AGG 0.579791 5_1:+44799778 Msa0732400:CDS
CAGGAATGAGTTGATGGAGA+AGG 0.584159 5_1:-44799854 None:intergenic
TCTTCCATTCTAAGAAGCAA+AGG 0.595126 5_1:+44799017 Msa0732400:CDS
AAAGGTTCAATCAATAAGCA+TGG 0.595162 5_1:+44800515 Msa0732400:CDS
ACAAAGATTACCAACATTGG+AGG 0.598469 5_1:+44799573 Msa0732400:CDS
ATGGAAGAAGGTGAGAATGG+TGG 0.608866 5_1:-44799002 None:intergenic
GTTGGAGACTCTCTCAATCG+TGG 0.612428 5_1:+44799810 Msa0732400:CDS
GTGGTTGCCACCTCCAATGT+TGG 0.612456 5_1:-44799583 None:intergenic
AATTGACTTGCCAAGAACAT+GGG 0.614611 5_1:+44799543 Msa0732400:CDS
AAGATTACCAACATTGGAGG+TGG 0.615305 5_1:+44799576 Msa0732400:CDS
TATGAAAAGTATGCTTAGAT+GGG 0.615716 5_1:+44800800 Msa0732400:CDS
TCAAGGTGCCATTTGCGGTG+GGG 0.620684 5_1:+44799420 Msa0732400:CDS
GGATACACGACCACTGTACG+AGG 0.634784 5_1:+44799493 Msa0732400:CDS
AAGCATGGTAGAAATTGGAA+AGG 0.640034 5_1:+44800530 Msa0732400:CDS
CATTGGAGGTGGCAACCACA+CGG 0.642871 5_1:+44799587 Msa0732400:CDS
TCTCCATCAACTCATTCCTG+AGG 0.645543 5_1:+44799857 Msa0732400:CDS
AAAGTATGCTTAGATGGGTG+AGG 0.645663 5_1:+44800805 Msa0732400:CDS
GATTGTCACAATTTCAACTG+AGG 0.649875 5_1:+44800761 Msa0732400:CDS
TTAGGAAGATCGCAACCGTG+TGG 0.699752 5_1:-44799602 None:intergenic
CGGTGGGGAAGACAGAGGAA+GGG 0.705520 5_1:+44799435 Msa0732400:CDS
ATTTGCGGTGGGGAAGACAG+AGG 0.711107 5_1:+44799430 Msa0732400:CDS
TTGATGCTTGAAACACTACG+TGG 0.805901 5_1:+44799771 Msa0732400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAATAATTATATTTAG+TGG - chr5_1:44800299-44800318 None:intergenic 10.0%
!!! TAATTTTCAAATAATCAAAA+TGG - chr5_1:44800677-44800696 None:intergenic 10.0%
!! TTAAAATCTTATGAATATGT+CGG - chr5_1:44799295-44799314 None:intergenic 15.0%
!!! AATATATTATGCCTTTTTTA+TGG + chr5_1:44799338-44799357 Msa0732400:intron 15.0%
!!! TTTATTTGATGTTTTGTATT+TGG + chr5_1:44799361-44799380 Msa0732400:intron 15.0%
!!! TTATTTGATGTTTTGTATTT+GGG + chr5_1:44799362-44799381 Msa0732400:intron 15.0%
!! TTCACTTTAATGTAATAATT+TGG + chr5_1:44800162-44800181 Msa0732400:intron 15.0%
!! TGAAAATCAACAATAAAAAA+TGG - chr5_1:44800383-44800402 None:intergenic 15.0%
!! AAGTCTAAAAAAATTGAAAA+GGG - chr5_1:44800715-44800734 None:intergenic 15.0%
!!! TTTTACATCAAAAAGTATAG+AGG + chr5_1:44799228-44799247 Msa0732400:intron 20.0%
!! TAAAATCTTATGAATATGTC+GGG - chr5_1:44799294-44799313 None:intergenic 20.0%
!! CATCAAATAAACCATAAAAA+AGG - chr5_1:44799352-44799371 None:intergenic 20.0%
!!! TATTTGATGTTTTGTATTTG+GGG + chr5_1:44799363-44799382 Msa0732400:intron 20.0%
!! GCATAAATAAATACTTACTT+AGG - chr5_1:44799623-44799642 None:intergenic 20.0%
!! TTCATAACAATAATGAAGAT+CGG - chr5_1:44799950-44799969 None:intergenic 20.0%
!! TTCATTATTGTTATGAATGA+TGG + chr5_1:44799953-44799972 Msa0732400:intron 20.0%
!! AGTTAATTATAAGAGTGAAT+CGG + chr5_1:44800098-44800117 Msa0732400:intron 20.0%
!! CAAATAATCAAAATGGAAAA+GGG - chr5_1:44800670-44800689 None:intergenic 20.0%
!! TCAAATAATCAAAATGGAAA+AGG - chr5_1:44800671-44800690 None:intergenic 20.0%
!! CAAGTCTAAAAAAATTGAAA+AGG - chr5_1:44800716-44800735 None:intergenic 20.0%
!!! CTTTTCAATTTTTTTAGACT+TGG + chr5_1:44800714-44800733 Msa0732400:intron 20.0%
! AAAAAGAATGAAAGCTAGAA+GGG - chr5_1:44799066-44799085 None:intergenic 25.0%
!! CTTTTTGTTACTATTCTCTA+TGG + chr5_1:44799080-44799099 Msa0732400:CDS 25.0%
!!! TGTAGCATTTTTTGAAACAA+TGG - chr5_1:44799186-44799205 None:intergenic 25.0%
! AAAATCTTATGAATATGTCG+GGG - chr5_1:44799293-44799312 None:intergenic 25.0%
!! ATTTGATGTTTTGTATTTGG+GGG + chr5_1:44799364-44799383 Msa0732400:intron 25.0%
! TTGATAAAAGAACGAGATAA+TGG + chr5_1:44800065-44800084 Msa0732400:intron 25.0%
!! TGATTTTCATGTGTTTAATC+AGG + chr5_1:44800394-44800413 Msa0732400:intron 25.0%
!!! TTTTCTTCTAGAATCAAACT+CGG + chr5_1:44800451-44800470 Msa0732400:CDS 25.0%
! TCTGATAGAATTGTAAGAAA+AGG + chr5_1:44800497-44800516 Msa0732400:CDS 25.0%
!!! ATCTTTTGATGATGAAGTAA+AGG + chr5_1:44800737-44800756 Msa0732400:CDS 25.0%
!! TATGAAAAGTATGCTTAGAT+GGG + chr5_1:44800800-44800819 Msa0732400:CDS 25.0%
CAAAAAGAATGAAAGCTAGA+AGG - chr5_1:44799067-44799086 None:intergenic 30.0%
! TTTGATGTTTTGTATTTGGG+GGG + chr5_1:44799365-44799384 Msa0732400:intron 30.0%
TGATATATTCAATGGAAGGT+GGG + chr5_1:44799460-44799479 Msa0732400:CDS 30.0%
GAAAACAGTTAGTGAATCAA+AGG - chr5_1:44799901-44799920 None:intergenic 30.0%
AGAAATCATAAGTATTCGTG+TGG + chr5_1:44800012-44800031 Msa0732400:intron 30.0%
GAAATCATAAGTATTCGTGT+GGG + chr5_1:44800013-44800032 Msa0732400:intron 30.0%
AATCAACTAGAGTTAGTTGT+TGG - chr5_1:44800144-44800163 None:intergenic 30.0%
ATGCAACAATTGAGTTCTAT+TGG + chr5_1:44800423-44800442 Msa0732400:CDS 30.0%
TGCAACAATTGAGTTCTATT+GGG + chr5_1:44800424-44800443 Msa0732400:CDS 30.0%
! GAGTTTGATTCTAGAAGAAA+AGG - chr5_1:44800452-44800471 None:intergenic 30.0%
AAAGGTTCAATCAATAAGCA+TGG + chr5_1:44800515-44800534 Msa0732400:CDS 30.0%
TCAATAAGCATGGTAGAAAT+TGG + chr5_1:44800525-44800544 Msa0732400:CDS 30.0%
TGGTATTCAACACTTATCTT+TGG + chr5_1:44800564-44800583 Msa0732400:CDS 30.0%
TATTCAACACTTATCTTTGG+TGG + chr5_1:44800567-44800586 Msa0732400:CDS 30.0%
GCTTGTTTGAATAGAAAAGA+TGG - chr5_1:44800626-44800645 None:intergenic 30.0%
! CTATGAAAAGTATGCTTAGA+TGG + chr5_1:44800799-44800818 Msa0732400:CDS 30.0%
!!! AAGAAGACTCTAGTTTTCTT+TGG - chr5_1:44800843-44800862 None:intergenic 30.0%
TTGCTTCTTAGAATGGAAGA+AGG - chr5_1:44799017-44799036 None:intergenic 35.0%
! TCTTCCATTCTAAGAAGCAA+AGG + chr5_1:44799017-44799036 Msa0732400:CDS 35.0%
GAAGATTTCATGTGCATCTT+TGG + chr5_1:44799104-44799123 Msa0732400:CDS 35.0%
GGGTGAATAATGAAAAGAGA+GGG + chr5_1:44799385-44799404 Msa0732400:intron 35.0%
! GAAGGGTGTGATATATTCAA+TGG + chr5_1:44799452-44799471 Msa0732400:CDS 35.0%
GGTGTGATATATTCAATGGA+AGG + chr5_1:44799456-44799475 Msa0732400:CDS 35.0%
GTGATATATTCAATGGAAGG+TGG + chr5_1:44799459-44799478 Msa0732400:CDS 35.0%
GTCAATTGAGGTTGTATGTA+CGG - chr5_1:44799530-44799549 None:intergenic 35.0%
!! AATTGACTTGCCAAGAACAT+GGG + chr5_1:44799543-44799562 Msa0732400:CDS 35.0%
!! CAATGTTGGTAATCTTTGTC+GGG - chr5_1:44799572-44799591 None:intergenic 35.0%
!! CCAATGTTGGTAATCTTTGT+CGG - chr5_1:44799573-44799592 None:intergenic 35.0%
ACAAAGATTACCAACATTGG+AGG + chr5_1:44799573-44799592 Msa0732400:CDS 35.0%
ATCAAAGGTTTCCATGGATT+TGG - chr5_1:44799886-44799905 None:intergenic 35.0%
TAGTGAATCAAAGGTTTCCA+TGG - chr5_1:44799892-44799911 None:intergenic 35.0%
! ACTAACTGTTTTCACAGCAA+AGG + chr5_1:44799908-44799927 Msa0732400:CDS 35.0%
TTCACAGCAAAGGTAAATCA+AGG + chr5_1:44799918-44799937 Msa0732400:intron 35.0%
! ACTAGAGTTAGTTGTTGGTT+TGG - chr5_1:44800139-44800158 None:intergenic 35.0%
AAGCATGGTAGAAATTGGAA+AGG + chr5_1:44800530-44800549 Msa0732400:CDS 35.0%
!!! TTGGAAAGGTGTTGACATTT+TGG + chr5_1:44800544-44800563 Msa0732400:CDS 35.0%
GATTGTCACAATTTCAACTG+AGG + chr5_1:44800761-44800780 Msa0732400:CDS 35.0%
AGAATGGAAGAAGGTGAGAA+TGG - chr5_1:44799008-44799027 None:intergenic 40.0%
ACGACCTTTGCTTCTTAGAA+TGG - chr5_1:44799024-44799043 None:intergenic 40.0%
TAGAAGGGTGAAAAGGTAAG+GGG - chr5_1:44799051-44799070 None:intergenic 40.0%
CTAGAAGGGTGAAAAGGTAA+GGG - chr5_1:44799052-44799071 None:intergenic 40.0%
TGAAAGCTAGAAGGGTGAAA+AGG - chr5_1:44799058-44799077 None:intergenic 40.0%
GTGTGGAGAAGAATTTGTCT+TGG - chr5_1:44799154-44799173 None:intergenic 40.0%
! TTTGAAACAATGGACTCACC+AGG - chr5_1:44799176-44799195 None:intergenic 40.0%
GCGAGTTTGAACTCTCTTAT+GGG - chr5_1:44799269-44799288 None:intergenic 40.0%
GGGGTGAATAATGAAAAGAG+AGG + chr5_1:44799384-44799403 Msa0732400:intron 40.0%
! TATTCAATGGAAGGTGGGTT+TGG + chr5_1:44799465-44799484 Msa0732400:CDS 40.0%
! ATTCAATGGAAGGTGGGTTT+GGG + chr5_1:44799466-44799485 Msa0732400:CDS 40.0%
TGTTCTTGGCAAGTCAATTG+AGG - chr5_1:44799542-44799561 None:intergenic 40.0%
!! CAATTGACTTGCCAAGAACA+TGG + chr5_1:44799542-44799561 Msa0732400:CDS 40.0%
CCGACAAAGATTACCAACAT+TGG + chr5_1:44799570-44799589 Msa0732400:CDS 40.0%
AAGATTACCAACATTGGAGG+TGG + chr5_1:44799576-44799595 Msa0732400:CDS 40.0%
TTGATGCTTGAAACACTACG+TGG + chr5_1:44799771-44799790 Msa0732400:CDS 40.0%
TTGAAACACTACGTGGAAAG+AGG + chr5_1:44799778-44799797 Msa0732400:CDS 40.0%
GGAAAGAGGATGATGTTTGT+TGG + chr5_1:44799792-44799811 Msa0732400:CDS 40.0%
TAGAGATCATAGTGATCAGC+AGG + chr5_1:44799987-44800006 Msa0732400:intron 40.0%
! ACTTATCTTTGGTGGATGAC+TGG + chr5_1:44800575-44800594 Msa0732400:CDS 40.0%
AACTGAGGATGCTTATCGTA+TGG + chr5_1:44800776-44800795 Msa0732400:CDS 40.0%
AAAGTATGCTTAGATGGGTG+AGG + chr5_1:44800805-44800824 Msa0732400:CDS 40.0%
ATGGAAGAAGGTGAGAATGG+TGG - chr5_1:44799005-44799024 None:intergenic 45.0%
GCTAGAAGGGTGAAAAGGTA+AGG - chr5_1:44799053-44799072 None:intergenic 45.0%
GACAAATTCTTCTCCACACC+TGG + chr5_1:44799155-44799174 Msa0732400:CDS 45.0%
CGCGAGTTTGAACTCTCTTA+TGG - chr5_1:44799270-44799289 None:intergenic 45.0%
!! CAGGAATGAGTTGATGGAGA+AGG - chr5_1:44799857-44799876 None:intergenic 45.0%
TCTCCATCAACTCATTCCTG+AGG + chr5_1:44799857-44799876 Msa0732400:CDS 45.0%
! CATCCTCAGGAATGAGTTGA+TGG - chr5_1:44799863-44799882 None:intergenic 45.0%
CAAGCAACAACGTTAACACG+CGG - chr5_1:44800205-44800224 None:intergenic 45.0%
CGGACAATGCTGTTGTTCAT+AGG + chr5_1:44800471-44800490 Msa0732400:CDS 45.0%
ACTAGCATGTCACCTTCTCA+TGG + chr5_1:44800864-44800883 Msa0732400:CDS 45.0%
! AACAATGGACTCACCAGGTG+TGG - chr5_1:44799171-44799190 None:intergenic 50.0%
GAGGGTGAAGAAGCAGATCA+AGG + chr5_1:44799403-44799422 Msa0732400:CDS 50.0%
!! GCAGATCAAGGTGCCATTTG+CGG + chr5_1:44799415-44799434 Msa0732400:CDS 50.0%
!! ATCAAGGTGCCATTTGCGGT+GGG + chr5_1:44799419-44799438 Msa0732400:CDS 50.0%
ACGACCACTGTACGAGGAAT+CGG + chr5_1:44799499-44799518 Msa0732400:CDS 50.0%
TTAGGAAGATCGCAACCGTG+TGG - chr5_1:44799605-44799624 None:intergenic 50.0%
GTTGGAGACTCTCTCAATCG+TGG + chr5_1:44799810-44799829 Msa0732400:CDS 50.0%
TCCTGAGGATGCCAAATCCA+TGG + chr5_1:44799872-44799891 Msa0732400:CDS 50.0%
TCCATGGATTTGGCATCCTC+AGG - chr5_1:44799876-44799895 None:intergenic 50.0%
GTTAACACGCGGAGGATACT+TGG - chr5_1:44800194-44800213 None:intergenic 50.0%
! TCTTTGGTGGATGACTGGCT+TGG + chr5_1:44800580-44800599 Msa0732400:CDS 50.0%
!! GATCAAGGTGCCATTTGCGG+TGG + chr5_1:44799418-44799437 Msa0732400:CDS 55.0%
!! TCAAGGTGCCATTTGCGGTG+GGG + chr5_1:44799420-44799439 Msa0732400:CDS 55.0%
TCTGTCTTCCCCACCGCAAA+TGG - chr5_1:44799431-44799450 None:intergenic 55.0%
ATTTGCGGTGGGGAAGACAG+AGG + chr5_1:44799430-44799449 Msa0732400:CDS 55.0%
GGATACACGACCACTGTACG+AGG + chr5_1:44799493-44799512 Msa0732400:CDS 55.0%
CACTCCGATTCCTCGTACAG+TGG - chr5_1:44799506-44799525 None:intergenic 55.0%
TGTCGGGTCTCCCATGTTCT+TGG - chr5_1:44799556-44799575 None:intergenic 55.0%
GTGGTTGCCACCTCCAATGT+TGG - chr5_1:44799586-44799605 None:intergenic 55.0%
CATTGGAGGTGGCAACCACA+CGG + chr5_1:44799587-44799606 Msa0732400:CDS 55.0%
GCAACAACGTTAACACGCGG+AGG - chr5_1:44800202-44800221 None:intergenic 55.0%
CGGTGGGGAAGACAGAGGAA+GGG + chr5_1:44799435-44799454 Msa0732400:CDS 60.0%
! TGGAAGGTGGGTTTGGGACG+AGG + chr5_1:44799472-44799491 Msa0732400:CDS 60.0%
GCGGTGGGGAAGACAGAGGA+AGG + chr5_1:44799434-44799453 Msa0732400:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 44798972 44800893 44798972 ID=Msa0732400;Name=Msa0732400
chr5_1 mRNA 44798972 44800893 44798972 ID=Msa0732400-mRNA-1;Parent=Msa0732400;Name=Msa0732400-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0.25|0.2|1|1|1|5|0|320
chr5_1 exon 44798972 44799176 44798972 ID=Msa0732400-mRNA-1:exon:12717;Parent=Msa0732400-mRNA-1
chr5_1 exon 44799402 44799624 44799402 ID=Msa0732400-mRNA-1:exon:12718;Parent=Msa0732400-mRNA-1
chr5_1 exon 44799758 44799929 44799758 ID=Msa0732400-mRNA-1:exon:12719;Parent=Msa0732400-mRNA-1
chr5_1 exon 44800416 44800615 44800416 ID=Msa0732400-mRNA-1:exon:12720;Parent=Msa0732400-mRNA-1
chr5_1 exon 44800731 44800893 44800731 ID=Msa0732400-mRNA-1:exon:12721;Parent=Msa0732400-mRNA-1
chr5_1 CDS 44798972 44799176 44798972 ID=Msa0732400-mRNA-1:cds;Parent=Msa0732400-mRNA-1
chr5_1 CDS 44799402 44799624 44799402 ID=Msa0732400-mRNA-1:cds;Parent=Msa0732400-mRNA-1
chr5_1 CDS 44799758 44799929 44799758 ID=Msa0732400-mRNA-1:cds;Parent=Msa0732400-mRNA-1
chr5_1 CDS 44800416 44800615 44800416 ID=Msa0732400-mRNA-1:cds;Parent=Msa0732400-mRNA-1
chr5_1 CDS 44800731 44800893 44800731 ID=Msa0732400-mRNA-1:cds;Parent=Msa0732400-mRNA-1
Gene Sequence

>Msa0732400

GGATTTGGTGAATGCATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCACTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGTGA
Protein sequence

>Msa0732400

GFGECMQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGK*