AlfalfaGEDB | Msa1445050

M. sativa cultivar zhongmu-4 / Msa1445050

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044036 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1990538 GO:1990937
PFAM: PF14416.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445050	sp\|F4IH21\|TBL33_ARATH	78.537	410	86	1	17	426	17	424	0.0	689

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445050	G7K771	99.297	427	3	0	1	427	1	427	0.0	890

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0040660	Msa1445050	0.820687	6.011715e-53	-8.615850e-47
Msa0101340	Msa1445050	0.827412	1.587437e-54	-8.615850e-47
Msa0145680	Msa1445050	0.826668	2.390278e-54	-8.615850e-47
Msa0174450	Msa1445050	0.815502	8.945615e-52	-8.615850e-47
Msa0184680	Msa1445050	0.801337	9.443033e-49	-8.615850e-47
Msa0193850	Msa1445050	0.864510	1.154424e-64	-8.615850e-47
Msa0235710	Msa1445050	0.868586	5.850129e-66	-8.615850e-47
Msa0243600	Msa1445050	0.812914	3.335588e-51	-8.615850e-47
Msa0274440	Msa1445050	0.857605	1.456803e-62	-8.615850e-47
Msa0282220	Msa1445050	0.825842	3.759171e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445050	MtrunA17_Chr5g0437601	99.297	427	3	0	1	427	1	427	0.0	890
Msa1445050	MtrunA17_Chr3g0114101	74.596	433	101	5	4	427	1	433	0.0	660
Msa1445050	MtrunA17_Chr4g0005491	51.003	349	165	3	83	426	62	409	2.44e-132	387
Msa1445050	MtrunA17_Chr7g0243301	48.677	378	183	5	53	425	39	410	4.74e-132	386
Msa1445050	MtrunA17_Chr4g0005481	52.187	343	161	2	79	421	104	443	3.05e-129	380
Msa1445050	MtrunA17_Chr4g0006991	48.986	345	174	2	81	424	84	427	9.86e-129	378
Msa1445050	MtrunA17_Chr8g0360521	49.025	359	175	4	71	425	56	410	5.42e-128	376
Msa1445050	MtrunA17_Chr3g0113671	51.289	349	164	4	80	424	103	449	1.25e-126	374
Msa1445050	MtrunA17_Chr5g0437291	48.571	350	175	3	81	425	163	512	9.06e-119	356
Msa1445050	MtrunA17_Chr7g0242701	41.647	425	238	4	10	424	3	427	1.09e-118	352

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445050	AT2G40320.1	78.537	410	86	1	17	426	17	424	0.0	689
Msa1445050	AT3G11030.1	62.723	448	137	4	8	427	6	451	0.0	588
Msa1445050	AT5G01620.2	50.000	350	171	2	73	421	100	446	4.44e-129	380
Msa1445050	AT5G01620.1	50.000	350	171	2	73	421	100	446	4.44e-129	380
Msa1445050	AT5G01620.3	50.000	350	171	2	73	421	108	454	1.00e-128	379
Msa1445050	AT3G55990.1	53.009	349	158	3	81	425	138	484	4.94e-128	379
Msa1445050	AT1G73140.1	44.444	378	193	3	49	425	45	406	7.02e-127	373
Msa1445050	AT5G01360.2	48.696	345	176	1	81	424	34	378	1.68e-126	371
Msa1445050	AT2G40150.1	51.233	365	163	4	73	424	58	420	5.92e-126	371
Msa1445050	AT5G01360.1	48.696	345	176	1	81	424	90	434	6.81e-126	371

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Find 135 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATAATGCAAGTGAT+TGG	0.480921	tig0030914:-13941	Msa1445050:CDS
AAAGGTTCAATCAATAAGCA+TGG	0.595162	tig0030914:-14889	Msa1445050:CDS
AAAGTATGCTTAGATGGGTG+AGG	0.645663	tig0030914:-14599	Msa1445050:CDS
AAATTAGTTATACGTATATG+AGG	0.427945	tig0030914:-16691	Msa1445050:five_prime_UTR
AACAATGGACTCACCAGGTG+TGG	0.570501	tig0030914:+16236	None:intergenic
AACTGAGGATGCTTATCGTA+TGG	0.502726	tig0030914:-14628	Msa1445050:CDS
AAGATTACCAACATTGGAGG+TGG	0.615305	tig0030914:-15828	Msa1445050:CDS
AAGCATGGTAGAAATTGGAA+AGG	0.640034	tig0030914:-14874	Msa1445050:CDS
AAGGAAGAAAGAAATGAATG+AGG	0.563328	tig0030914:-13540	Msa1445050:three_prime_UTR
AATTAGTTATACGTATATGA+GGG	0.618030	tig0030914:-16690	Msa1445050:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAACTTATGTCTTTGTTGA+TGG	+	tig0030914:13429-13448	None:intergenic	30.0%
CTAGTATATTAGGAGAAAGT+GGG	+	tig0030914:13467-13486	None:intergenic	30.0%
ACTAGTATATTAGGAGAAAG+TGG	+	tig0030914:13468-13487	None:intergenic	30.0%
CAAAAAGAATGAAAGCTAGA+AGG	+	tig0030914:13679-13698	None:intergenic	30.0%
TGATATATTCAATGGAAGGT+GGG	-	tig0030914:14072-14091	Msa1445050:intron	30.0%
GAAAACAGTTAGTGAATCAA+AGG	+	tig0030914:14513-14532	None:intergenic	30.0%
AGAAATCATAAGTATTCGTG+TGG	-	tig0030914:14624-14643	Msa1445050:CDS	30.0%
GAAATCATAAGTATTCGTGT+GGG	-	tig0030914:14625-14644	Msa1445050:CDS	30.0%
AATCAACTAGAGTTAGTTGT+TGG	+	tig0030914:14756-14775	None:intergenic	30.0%
ATGCAACAATTGAGTTCTAT+TGG	-	tig0030914:15035-15054	Msa1445050:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030914	gene	13310	16728	13310	ID=Msa1445050;Name=Msa1445050
tig0030914	mRNA	13310	16728	13310	ID=Msa1445050-mRNA-1;Parent=Msa1445050;Name=Msa1445050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=289\|1\|1\|1\|1\|1\|6\|405\|427
tig0030914	exon	13310	14054	13310	ID=Msa1445050-mRNA-1:exon:14800;Parent=Msa1445050-mRNA-1
tig0030914	exon	14537	14695	14537	ID=Msa1445050-mRNA-1:exon:14799;Parent=Msa1445050-mRNA-1
tig0030914	exon	14811	15010	14811	ID=Msa1445050-mRNA-1:exon:14798;Parent=Msa1445050-mRNA-1
tig0030914	exon	15497	15668	15497	ID=Msa1445050-mRNA-1:exon:14797;Parent=Msa1445050-mRNA-1
tig0030914	exon	15802	16024	15802	ID=Msa1445050-mRNA-1:exon:14796;Parent=Msa1445050-mRNA-1
tig0030914	exon	16250	16728	16250	ID=Msa1445050-mRNA-1:exon:14795;Parent=Msa1445050-mRNA-1
tig0030914	five_prime_UTR	16440	16728	16440	ID=Msa1445050-mRNA-1:five_prime_utr;Parent=Msa1445050-mRNA-1
tig0030914	CDS	16250	16439	16250	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	CDS	15802	16024	15802	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	CDS	15497	15668	15497	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	CDS	14811	15010	14811	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	CDS	14537	14695	14537	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	CDS	13715	14054	13715	ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914	three_prime_UTR	13310	13714	13310	ID=Msa1445050-mRNA-1:three_prime_utr;Parent=Msa1445050-mRNA-1

Gene Sequence

>Msa1445050

ATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCATTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGAGTATAGATTGGGGAGGTGAGCCAGGAGGAAGTTGTTACAATGAAACAACTTTGATTAATAATTCCACATACTGGGGTTCTGATTGTAGAAAAAGTATAATGCAAGTGATTGGTGAAGTGTTAAGCAAAACAAAAGTTCCAATTACATTTCTTAACATCACACAACTCTCAAGTTACCGTAAAGATGCACACACTTCAATCTATAAGAAGCAATGGAGCCCTTTAACTAAAGAGCAATTAGCTAACCCTGTAAGCTATGCTGATTGTGTACATTGGTGTTTACCTGGTCTTCAAGATAATTGGAATGAACTTTTGTTTGCTAAGTTGTTTTATCCTTGA

Protein sequence

>Msa1445050

MQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGKSIDWGGEPGGSCYNETTLINNSTYWGSDCRKSIMQVIGEVLSKTKVPITFLNITQLSSYRKDAHTSIYKKQWSPLTKEQLANPVSYADCVHWCLPGLQDNWNELLFAKLFYP*