AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1445050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1445050 MtrunA17_Chr5g0437601 99.297 427 3 0 1 427 1 427 0.0 890
Msa1445050 MtrunA17_Chr3g0114101 74.596 433 101 5 4 427 1 433 0.0 660
Msa1445050 MtrunA17_Chr4g0005491 51.003 349 165 3 83 426 62 409 2.44e-132 387
Msa1445050 MtrunA17_Chr7g0243301 48.677 378 183 5 53 425 39 410 4.74e-132 386
Msa1445050 MtrunA17_Chr4g0005481 52.187 343 161 2 79 421 104 443 3.05e-129 380
Msa1445050 MtrunA17_Chr4g0006991 48.986 345 174 2 81 424 84 427 9.86e-129 378
Msa1445050 MtrunA17_Chr8g0360521 49.025 359 175 4 71 425 56 410 5.42e-128 376
Msa1445050 MtrunA17_Chr3g0113671 51.289 349 164 4 80 424 103 449 1.25e-126 374
Msa1445050 MtrunA17_Chr5g0437291 48.571 350 175 3 81 425 163 512 9.06e-119 356
Msa1445050 MtrunA17_Chr7g0242701 41.647 425 238 4 10 424 3 427 1.09e-118 352
Msa1445050 MtrunA17_Chr5g0430371 36.675 379 207 9 64 426 40 401 1.24e-76 244
Msa1445050 MtrunA17_Chr3g0105301 36.944 360 201 8 76 426 36 378 1.57e-76 243
Msa1445050 MtrunA17_Chr5g0413271 36.932 352 206 8 77 425 139 477 2.08e-75 243
Msa1445050 MtrunA17_Chr4g0041011 34.097 393 226 9 32 424 4 363 3.49e-75 239
Msa1445050 MtrunA17_Chr1g0210771 38.329 347 186 10 83 424 59 382 1.11e-74 238
Msa1445050 MtrunA17_Chr2g0325481 37.026 343 191 7 83 425 50 367 2.62e-74 236
Msa1445050 MtrunA17_Chr1g0175671 36.723 354 200 8 83 425 170 510 1.90e-73 239
Msa1445050 MtrunA17_Chr3g0097891 35.127 353 203 8 75 425 23 351 1.39e-72 231
Msa1445050 MtrunA17_Chr7g0258851 37.255 357 194 9 83 425 248 588 1.79e-72 238
Msa1445050 MtrunA17_Chr1g0171171 33.668 398 234 10 27 424 8 375 6.79e-72 231
Msa1445050 MtrunA17_Chr7g0259711 33.034 445 258 15 2 425 8 433 1.59e-71 232
Msa1445050 MtrunA17_Chr4g0071391 37.143 350 200 8 83 424 109 446 2.05e-71 232
Msa1445050 MtrunA17_Chr2g0287901 37.183 355 196 11 81 422 95 435 5.72e-70 228
Msa1445050 MtrunA17_Chr2g0314801 36.286 350 192 8 77 421 27 350 9.66e-69 222
Msa1445050 MtrunA17_Chr2g0283581 34.925 398 211 11 27 424 9 358 2.57e-68 221
Msa1445050 MtrunA17_Chr7g0269991 36.648 352 192 9 83 424 137 467 5.71e-68 223
Msa1445050 MtrunA17_Chr3g0085081 35.817 349 190 10 81 424 29 348 1.29e-67 219
Msa1445050 MtrunA17_Chr5g0413851 37.644 348 189 10 79 424 38 359 1.54e-67 219
Msa1445050 MtrunA17_Chr4g0045941 33.903 351 208 8 75 425 25 351 1.55e-66 216
Msa1445050 MtrunA17_Chr3g0085071 35.817 349 190 10 81 424 44 363 2.31e-66 216
Msa1445050 MtrunA17_Chr1g0213281 33.943 383 231 10 56 426 145 517 2.39e-66 220
Msa1445050 MtrunA17_Chr4g0068111 33.711 353 212 7 83 425 114 454 7.10e-66 218
Msa1445050 MtrunA17_Chr3g0091031 35.244 349 192 10 81 424 29 348 2.71e-65 213
Msa1445050 MtrunA17_Chr8g0368461 33.333 372 213 10 76 425 171 529 3.28e-63 213
Msa1445050 MtrunA17_Chr4g0045951 33.333 348 208 8 78 425 40 363 1.50e-61 203
Msa1445050 MtrunA17_Chr2g0314791 35.393 356 185 13 80 425 26 346 2.01e-60 200
Msa1445050 MtrunA17_Chr8g0384151 28.440 436 260 13 5 425 19 417 6.83e-55 188
Msa1445050 MtrunA17_Chr3g0126021 29.609 358 219 8 79 425 125 460 2.06e-52 182
Msa1445050 MtrunA17_Chr6g0456681 30.833 360 220 10 81 424 62 408 3.83e-43 156
Msa1445050 MtrunA17_Chr1g0209921 34.043 235 143 6 81 313 42 266 3.91e-42 149
Msa1445050 MtrunA17_Chr1g0188191 27.889 398 254 16 43 421 195 578 4.66e-40 150
Msa1445050 MtrunA17_Chr1g0178081 29.261 352 180 9 81 425 53 342 5.44e-40 146
Msa1445050 MtrunA17_Chr6g0456691 27.419 434 262 16 16 424 14 419 3.69e-39 145
Msa1445050 MtrunA17_Chr8g0347321 28.571 357 228 12 83 424 95 439 6.88e-39 145
Msa1445050 MtrunA17_Chr8g0384421 30.028 363 214 13 81 424 131 472 2.46e-37 141
Msa1445050 MtrunA17_Chr6g0476041 28.011 357 231 14 81 421 165 511 3.29e-37 142
Msa1445050 MtrunA17_Chr8g0391481 29.428 367 219 15 80 424 24 372 2.38e-36 137
Msa1445050 MtrunA17_Chr6g0451321 28.032 371 229 14 73 424 106 457 5.76e-36 137
Msa1445050 MtrunA17_Chr5g0414061 27.887 355 227 12 83 421 94 435 4.22e-35 135
Msa1445050 MtrunA17_Chr5g0414091 28.453 362 222 12 80 420 82 427 8.64e-35 134
Msa1445050 MtrunA17_Chr2g0283851 27.559 381 230 15 65 420 37 396 8.87e-35 133
Msa1445050 MtrunA17_Chr5g0414051 25.886 367 226 13 80 421 72 417 4.38e-33 129
Msa1445050 MtrunA17_Chr3g0140371 28.333 360 223 13 80 421 15 357 9.76e-33 127
Msa1445050 MtrunA17_Chr7g0226991 27.348 362 227 14 80 421 54 399 1.43e-32 127
Msa1445050 MtrunA17_Chr8g0387921 26.479 355 231 12 83 420 74 415 2.71e-32 127
Msa1445050 MtrunA17_Chr5g0414041 28.571 357 224 12 83 421 87 430 1.44e-31 125
Msa1445050 MtrunA17_Chr4g0049421 26.997 363 218 16 83 420 48 388 1.69e-29 118
Msa1445050 MtrunA17_Chr6g0455901 26.503 366 231 12 80 424 68 416 9.03e-29 117
Msa1445050 MtrunA17_Chr4g0070341 27.704 379 202 16 81 424 130 471 2.22e-27 113
Msa1445050 MtrunA17_Chr7g0221271 40.000 130 77 1 81 210 77 205 4.19e-26 104
Msa1445050 MtrunA17_Chr7g0221291 45.361 97 52 1 79 175 75 170 1.71e-24 99.4
Msa1445050 MtrunA17_Chr2g0287931 36.134 119 66 4 143 252 1 118 1.77e-15 72.8
Msa1445050 MtrunA17_Chr3g0085101 38.318 107 62 3 280 385 5 108 6.20e-13 65.5
Msa1445050 MtrunA17_Chr7g0221281 25.311 241 155 10 197 424 8 236 7.06e-12 65.5
Msa1445050 MtrunA17_Chr2g0287911 43.750 64 28 2 76 139 54 109 1.23e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1445050 AT2G40320.1 78.537 410 86 1 17 426 17 424 0.0 689
Msa1445050 AT3G11030.1 62.723 448 137 4 8 427 6 451 0.0 588
Msa1445050 AT5G01620.2 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa1445050 AT5G01620.1 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa1445050 AT5G01620.3 50.000 350 171 2 73 421 108 454 1.00e-128 379
Msa1445050 AT3G55990.1 53.009 349 158 3 81 425 138 484 4.94e-128 379
Msa1445050 AT1G73140.1 44.444 378 193 3 49 425 45 406 7.02e-127 373
Msa1445050 AT5G01360.2 48.696 345 176 1 81 424 34 378 1.68e-126 371
Msa1445050 AT2G40150.1 51.233 365 163 4 73 424 58 420 5.92e-126 371
Msa1445050 AT5G01360.1 48.696 345 176 1 81 424 90 434 6.81e-126 371
Msa1445050 AT2G38320.1 44.865 370 198 3 61 425 37 405 8.04e-117 347
Msa1445050 AT2G40160.1 40.821 414 229 7 23 426 18 425 1.70e-107 324
Msa1445050 AT2G40160.2 41.176 408 224 7 23 420 18 419 4.32e-107 323
Msa1445050 AT2G38320.2 47.188 320 163 3 111 425 4 322 1.18e-105 316
Msa1445050 AT3G12060.1 38.259 379 204 9 61 425 174 536 3.80e-82 263
Msa1445050 AT5G06700.1 38.547 358 192 10 81 425 253 595 1.11e-78 255
Msa1445050 AT3G62390.1 37.166 374 203 11 68 426 117 473 1.97e-75 243
Msa1445050 AT1G78710.1 35.429 350 199 8 78 425 33 357 3.19e-75 239
Msa1445050 AT5G20590.1 36.639 363 209 8 72 425 130 480 3.68e-74 240
Msa1445050 AT2G40160.3 39.799 299 166 5 23 313 18 310 3.78e-74 234
Msa1445050 AT5G19160.1 34.651 430 237 14 20 422 30 442 5.21e-74 239
Msa1445050 AT3G14850.2 37.249 349 188 7 78 421 29 351 9.90e-73 233
Msa1445050 AT2G30900.1 35.135 370 204 12 68 426 23 367 4.13e-72 231
Msa1445050 AT3G06080.2 32.118 439 247 12 15 422 25 443 1.82e-70 230
Msa1445050 AT5G58600.1 35.411 353 203 7 83 426 66 402 2.22e-70 228
Msa1445050 AT5G49340.1 35.230 369 219 9 68 425 83 442 2.39e-70 229
Msa1445050 AT1G29050.1 34.582 347 203 6 78 424 56 378 4.46e-70 226
Msa1445050 AT2G30010.1 35.309 388 214 10 54 426 33 398 5.49e-70 227
Msa1445050 AT2G42570.1 34.140 372 217 9 54 425 22 365 1.17e-69 225
Msa1445050 AT2G30010.2 35.309 388 214 10 54 426 76 441 1.61e-69 227
Msa1445050 AT2G34070.1 34.473 351 199 8 78 424 60 383 2.36e-69 224
Msa1445050 AT2G31110.2 36.047 344 192 9 83 424 45 362 7.11e-69 223
Msa1445050 AT1G60790.1 34.551 356 212 9 81 425 188 533 2.02e-68 226
Msa1445050 AT3G54260.1 36.578 339 195 8 83 420 51 370 8.23e-67 218
Msa1445050 AT5G06230.2 34.426 366 196 11 83 425 21 365 3.10e-61 203
Msa1445050 AT5G06230.1 34.426 366 196 11 83 425 62 406 1.14e-60 203
Msa1445050 AT1G48880.1 30.095 422 232 14 23 425 63 440 4.01e-57 194
Msa1445050 AT1G78710.2 30.747 348 165 8 78 425 26 297 2.00e-55 186
Msa1445050 AT5G19160.2 34.505 313 186 8 20 317 30 338 1.45e-54 185
Msa1445050 AT3G11570.1 33.828 337 198 7 100 425 98 420 2.34e-54 186
Msa1445050 AT3G62390.2 38.113 265 143 8 68 317 117 375 9.30e-52 179
Msa1445050 AT3G06080.1 35.743 249 146 5 76 313 93 338 6.06e-50 172
Msa1445050 AT5G20590.2 37.450 251 144 5 72 313 130 376 1.88e-49 172
Msa1445050 AT2G30900.2 35.271 258 145 8 68 315 23 268 8.85e-47 162
Msa1445050 AT2G31110.3 35.088 228 135 5 83 308 45 261 1.98e-44 156
Msa1445050 AT1G70230.1 29.609 358 221 11 78 424 74 411 2.07e-43 157
Msa1445050 AT5G58600.2 33.473 239 140 5 83 315 66 291 1.16e-42 152
Msa1445050 AT2G34070.2 32.364 275 156 7 154 424 1 249 2.64e-42 150
Msa1445050 AT3G14850.1 34.066 273 150 6 154 421 1 248 4.96e-42 149
Msa1445050 AT3G28150.1 28.000 425 245 16 18 422 25 408 1.89e-41 152
Msa1445050 AT5G06230.3 34.524 252 134 7 83 313 62 303 9.25e-41 147
Msa1445050 AT5G15900.1 29.082 392 247 12 50 424 32 409 4.75e-40 148
Msa1445050 AT5G15900.2 29.082 392 247 12 50 424 51 428 5.81e-40 148
Msa1445050 AT5G15890.1 28.652 356 220 10 80 421 184 519 3.14e-38 145
Msa1445050 AT5G64020.1 29.301 372 213 16 83 424 57 408 1.11e-37 141
Msa1445050 AT5G64470.3 29.915 351 218 13 83 420 55 390 3.09e-37 140
Msa1445050 AT3G02440.1 26.966 356 232 11 83 424 127 468 7.38e-37 140
Msa1445050 AT3G02440.2 26.966 356 232 11 83 424 135 476 1.03e-36 140
Msa1445050 AT5G64470.2 29.494 356 219 12 83 420 55 396 2.05e-36 138
Msa1445050 AT1G01430.1 29.661 354 224 12 83 420 99 443 1.81e-35 136
Msa1445050 AT4G25360.1 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa1445050 AT4G25360.2 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa1445050 AT4G23790.1 25.059 427 276 17 21 424 22 427 8.86e-35 134
Msa1445050 AT5G51640.1 27.174 368 225 15 80 421 135 485 4.38e-34 133
Msa1445050 AT2G37720.1 28.571 385 218 17 66 424 129 482 5.56e-34 132
Msa1445050 AT4G01080.1 29.429 350 221 14 83 420 91 426 1.05e-33 131
Msa1445050 AT2G14530.1 26.519 362 229 10 83 424 62 406 1.17e-33 130
Msa1445050 AT2G31110.1 33.621 232 127 8 195 424 8 214 6.24e-31 118
Msa1445050 AT5G20680.6 26.178 382 208 16 79 424 114 457 1.06e-29 120
Msa1445050 AT5G20680.2 26.178 382 208 16 79 424 190 533 1.77e-29 120
Msa1445050 AT5G20680.4 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1445050 AT5G20680.1 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1445050 AT5G20680.3 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1445050 AT5G20680.5 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1445050 AT4G11090.1 23.040 421 288 15 23 421 16 422 7.15e-27 112
Msa1445050 AT5G64470.1 27.985 268 178 9 83 340 55 317 3.72e-23 99.8
Msa1445050 AT5G64470.4 27.715 267 178 9 83 339 55 316 1.27e-22 98.2

Find 135 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATGGAGTTCCCTCTTT+TGG 0.157557 tig0030914:+16654 None:intergenic
TACCTGGTCTTCAAGATAAT+TGG 0.191574 tig0030914:-13751 Msa1445050:CDS
TTGGAAAGGTGTTGACATTT+TGG 0.194283 tig0030914:-14860 Msa1445050:CDS
GCTAATTGCTCTTTAGTTAA+AGG 0.201296 tig0030914:+13812 None:intergenic
ACTCCATTGTGATGTCTTAT+TGG 0.212538 tig0030914:-16642 Msa1445050:five_prime_UTR
ACACATTTGTTCAAATATAA+AGG 0.225938 tig0030914:-13685 Msa1445050:three_prime_UTR
TGATTAATAATTCCACATAC+TGG 0.251655 tig0030914:-13979 Msa1445050:CDS
TGTCGGGTCTCCCATGTTCT+TGG 0.253030 tig0030914:+15851 None:intergenic
ATGCAACAATTGAGTTCTAT+TGG 0.254941 tig0030914:-14981 Msa1445050:CDS
GAATTTGGTATTTCAGTTAT+AGG 0.257936 tig0030914:+13437 None:intergenic
TCAATAAGCATGGTAGAAAT+TGG 0.262180 tig0030914:-14879 Msa1445050:CDS
ATTGAATGTACTAGTATATT+AGG 0.272300 tig0030914:+16542 None:intergenic
TTGAAGTGTGTGCATCTTTA+CGG 0.287806 tig0030914:+13855 None:intergenic
TGAATTATCTATATATTGTT+AGG 0.290624 tig0030914:-13354 Msa1445050:three_prime_UTR
ATCAAAGGTTTCCATGGATT+TGG 0.299044 tig0030914:+15521 None:intergenic
ATCATATATTGTTGTTCTCT+TGG 0.308084 tig0030914:-13661 Msa1445050:three_prime_UTR
CTAATTGCTCTTTAGTTAAA+GGG 0.317364 tig0030914:+13813 None:intergenic
GAAGATTTCATGTGCATCTT+TGG 0.320970 tig0030914:-16300 Msa1445050:CDS
TATTCAATGGAAGGTGGGTT+TGG 0.326490 tig0030914:-15939 Msa1445050:CDS
TGAACTTATGTCTTTGTTGA+TGG 0.335672 tig0030914:+16590 None:intergenic
CTCCATTGTGATGTCTTATT+GGG 0.337032 tig0030914:-16641 Msa1445050:five_prime_UTR
GTGATGTTAAGAAATGTAAT+TGG 0.337450 tig0030914:+13893 None:intergenic
TGGTATTCAACACTTATCTT+TGG 0.343420 tig0030914:-14840 Msa1445050:CDS
TCTCTTGGATCATGGACTCT+TGG 0.348285 tig0030914:-13646 Msa1445050:three_prime_UTR
ACACAATCAGCATAGCTTAC+AGG 0.357812 tig0030914:+13785 None:intergenic
CACATTTGTTCAAATATAAA+GGG 0.368100 tig0030914:-13684 Msa1445050:three_prime_UTR
ATTCAATGGAAGGTGGGTTT+GGG 0.383497 tig0030914:-15938 Msa1445050:CDS
CGGACAATGCTGTTGTTCAT+AGG 0.387335 tig0030914:-14933 Msa1445050:CDS
CTCTTGGATCATGGACTCTT+GGG 0.388166 tig0030914:-13645 Msa1445050:three_prime_UTR
ATTGTTGTTCTCTTGGATCA+TGG 0.391390 tig0030914:-13654 Msa1445050:three_prime_UTR
ACGACCTTTGCTTCTTAGAA+TGG 0.393423 tig0030914:+16383 None:intergenic
CCAATGTTGGTAATCTTTGT+CGG 0.403476 tig0030914:+15834 None:intergenic
CTATGAAAAGTATGCTTAGA+TGG 0.410597 tig0030914:-14605 Msa1445050:CDS
TCTGTCTTCCCCACCGCAAA+TGG 0.411531 tig0030914:+15976 None:intergenic
TGCAACAATTGAGTTCTATT+GGG 0.419007 tig0030914:-14980 Msa1445050:CDS
GGTATTTCAGTTATAGGAAA+GGG 0.419698 tig0030914:+13443 None:intergenic
TATTCAACACTTATCTTTGG+TGG 0.424745 tig0030914:-14837 Msa1445050:CDS
TCCATGGATTTGGCATCCTC+AGG 0.427436 tig0030914:+15531 None:intergenic
AAATTAGTTATACGTATATG+AGG 0.427945 tig0030914:-16691 Msa1445050:five_prime_UTR
ACTAGTATATTAGGAGAAAG+TGG 0.429923 tig0030914:+16551 None:intergenic
ACTTATCTTTGGTGGATGAC+TGG 0.435500 tig0030914:-14829 Msa1445050:CDS
ATATGGCAGGAGTATAGATT+GGG 0.440954 tig0030914:-14041 Msa1445050:intron
TATATGGCAGGAGTATAGAT+TGG 0.441176 tig0030914:-14042 Msa1445050:intron
TAAATAATAGTGAGTTGAGT+TGG 0.441530 tig0030914:-16453 Msa1445050:five_prime_UTR
ATAGATTGGGGAGGTGAGCC+AGG 0.443479 tig0030914:-14028 Msa1445050:CDS
GTTAGTAGTTGTTCAAAGAA+AGG 0.448939 tig0030914:-13504 Msa1445050:three_prime_UTR
ATAGTGAGTTGAGTTGGATT+TGG 0.450733 tig0030914:-16447 Msa1445050:five_prime_UTR
CATCCTCAGGAATGAGTTGA+TGG 0.450737 tig0030914:+15544 None:intergenic
CAATGTTGGTAATCTTTGTC+GGG 0.457780 tig0030914:+15835 None:intergenic
CACTCCGATTCCTCGTACAA+TGG 0.458569 tig0030914:+15901 None:intergenic
GCTAGAAGGGTGAAAAGGTA+AGG 0.463485 tig0030914:+16354 None:intergenic
TGAAAGCTAGAAGGGTGAAA+AGG 0.463624 tig0030914:+16349 None:intergenic
ACGACCATTGTACGAGGAAT+CGG 0.464432 tig0030914:-15905 Msa1445050:CDS
GATTAATAATTCCACATACT+GGG 0.472858 tig0030914:-13978 Msa1445050:CDS
TGTGTACATTGGTGTTTACC+TGG 0.477328 tig0030914:-13767 Msa1445050:CDS
TGGAAGGTGGGTTTGGGACG+AGG 0.477341 tig0030914:-15932 Msa1445050:CDS
CACAATCAGCATAGCTTACA+GGG 0.477761 tig0030914:+13786 None:intergenic
AAAAGTATAATGCAAGTGAT+TGG 0.480921 tig0030914:-13941 Msa1445050:CDS
TCTGATAGAATTGTAAGAAA+AGG 0.484458 tig0030914:-14907 Msa1445050:CDS
TGATTAATCTTTGTTTGCAA+AGG 0.491547 tig0030914:-13580 Msa1445050:three_prime_UTR
TAGTGAATCAAAGGTTTCCA+TGG 0.492850 tig0030914:+15515 None:intergenic
GGAAAGAGGATGATGTTTGT+TGG 0.493089 tig0030914:-15612 Msa1445050:CDS
GCAGATCAAGGTGCCATTTG+CGG 0.494304 tig0030914:-15989 Msa1445050:CDS
GAAGGGTGTGATATATTCAA+TGG 0.494550 tig0030914:-15952 Msa1445050:CDS
GTGATATATTCAATGGAAGG+TGG 0.495098 tig0030914:-15945 Msa1445050:CDS
TCTTTGGTGGATGACTGGCT+TGG 0.496770 tig0030914:-14824 Msa1445050:CDS
TTGTGATGTCTTATTGGGTA+AGG 0.497193 tig0030914:-16636 Msa1445050:five_prime_UTR
TCATTGTAACAACTTCCTCC+TGG 0.501058 tig0030914:+14010 None:intergenic
GAGTTTGATTCTAGAAGAAA+AGG 0.501756 tig0030914:+14955 None:intergenic
TGTTCTTGGCAAGTCAATTG+AGG 0.502642 tig0030914:+15865 None:intergenic
AACTGAGGATGCTTATCGTA+TGG 0.502726 tig0030914:-14628 Msa1445050:CDS
CTAGTATATTAGGAGAAAGT+GGG 0.504465 tig0030914:+16552 None:intergenic
CAATTGACTTGCCAAGAACA+TGG 0.509598 tig0030914:-15862 Msa1445050:CDS
GTCAATTGAGGTTGTATGTA+CGG 0.512274 tig0030914:+15877 None:intergenic
GACAAATTCTTCTCCACACC+TGG 0.515054 tig0030914:-16249 Msa1445050:intron
ACTAGCATGTCACCTTCTCA+TGG 0.524451 tig0030914:-14540 Msa1445050:CDS
ACCACTTAAGTGCCCAAAAG+AGG 0.525177 tig0030914:-16667 Msa1445050:five_prime_UTR
TTCCAATTATCTTGAAGACC+AGG 0.528264 tig0030914:+13749 None:intergenic
GAAAACAGTTAGTGAATCAA+AGG 0.528871 tig0030914:+15506 None:intergenic
TGATATATTCAATGGAAGGT+GGG 0.529302 tig0030914:-15944 Msa1445050:CDS
CTAGAAGGGTGAAAAGGTAA+GGG 0.530481 tig0030914:+16355 None:intergenic
AGAATGGAAGAAGGTGAGAA+TGG 0.531203 tig0030914:+16399 None:intergenic
GCGGTGGGGAAGACAGAGGA+AGG 0.532473 tig0030914:-15970 Msa1445050:CDS
ACAATCAGAACCCCAGTATG+TGG 0.541987 tig0030914:+13967 None:intergenic
TTGCTTCTTAGAATGGAAGA+AGG 0.542128 tig0030914:+16390 None:intergenic
CTTCAATCTATAAGAAGCAA+TGG 0.542329 tig0030914:-13838 Msa1445050:CDS
TGGTATTTCAGTTATAGGAA+AGG 0.542790 tig0030914:+13442 None:intergenic
TCCTGAGGATGCCAAATCCA+TGG 0.545590 tig0030914:-15532 Msa1445050:CDS
GTACTTTACTCTAAAAGACT+TGG 0.546625 tig0030914:+16498 None:intergenic
GAGGGTGAAGAAGCAGATCA+AGG 0.550519 tig0030914:-16001 Msa1445050:CDS
TATACTCACTTTCCATGAGA+AGG 0.556322 tig0030914:+14528 None:intergenic
ATCAAGGTGCCATTTGCGGT+GGG 0.557278 tig0030914:-15985 Msa1445050:CDS
GGTGTGATATATTCAATGGA+AGG 0.558509 tig0030914:-15948 Msa1445050:CDS
AAGGAAGAAAGAAATGAATG+AGG 0.563328 tig0030914:-13540 Msa1445050:three_prime_UTR
TAGAAGGGTGAAAAGGTAAG+GGG 0.565912 tig0030914:+16356 None:intergenic
GATCAAGGTGCCATTTGCGG+TGG 0.568008 tig0030914:-15986 Msa1445050:CDS
CCGACAAAGATTACCAACAT+TGG 0.570402 tig0030914:-15834 Msa1445050:CDS
AACAATGGACTCACCAGGTG+TGG 0.570501 tig0030914:+16236 None:intergenic
GTATTTCAGTTATAGGAAAG+GGG 0.570896 tig0030914:+13444 None:intergenic
GTGTGGAGAAGAATTTGTCT+TGG 0.571094 tig0030914:+16253 None:intergenic
TTGAAACACTACGTGGAAAG+AGG 0.579791 tig0030914:-15626 Msa1445050:CDS
CAGGAATGAGTTGATGGAGA+AGG 0.584159 tig0030914:+15550 None:intergenic
ATTAATAATTCCACATACTG+GGG 0.584467 tig0030914:-13977 Msa1445050:CDS
TATTTCAGTTATAGGAAAGG+GGG 0.584658 tig0030914:+13445 None:intergenic
CCACTTAAGTGCCCAAAAGA+GGG 0.592767 tig0030914:-16666 Msa1445050:five_prime_UTR
TCTTCCATTCTAAGAAGCAA+AGG 0.595126 tig0030914:-16387 Msa1445050:CDS
AAAGGTTCAATCAATAAGCA+TGG 0.595162 tig0030914:-14889 Msa1445050:CDS
ACAAAGATTACCAACATTGG+AGG 0.598469 tig0030914:-15831 Msa1445050:CDS
GGATACATGTGACGATAACA+AGG 0.600528 tig0030914:-13559 Msa1445050:three_prime_UTR
GTGACACTTTCATTTGTACA+CGG 0.608400 tig0030914:-13621 Msa1445050:three_prime_UTR
ATGGAAGAAGGTGAGAATGG+TGG 0.608866 tig0030914:+16402 None:intergenic
GTTGGAGACTCTCTCAATCG+TGG 0.612428 tig0030914:-15594 Msa1445050:CDS
GTGGTTGCCACCTCCAATGT+TGG 0.612456 tig0030914:+15821 None:intergenic
AATTGACTTGCCAAGAACAT+GGG 0.614611 tig0030914:-15861 Msa1445050:CDS
CACGGAAAAGAGTATAGCTA+CGG 0.614974 tig0030914:-13603 Msa1445050:three_prime_UTR
AAGATTACCAACATTGGAGG+TGG 0.615305 tig0030914:-15828 Msa1445050:CDS
TATGAAAAGTATGCTTAGAT+GGG 0.615716 tig0030914:-14604 Msa1445050:CDS
AATTAGTTATACGTATATGA+GGG 0.618030 tig0030914:-16690 Msa1445050:five_prime_UTR
TCAAGGTGCCATTTGCGGTG+GGG 0.620684 tig0030914:-15984 Msa1445050:CDS
TATGGCAGGAGTATAGATTG+GGG 0.621719 tig0030914:-14040 Msa1445050:intron
AAGCATGGTAGAAATTGGAA+AGG 0.640034 tig0030914:-14874 Msa1445050:CDS
CATTGGAGGTGGCAACCACA+CGG 0.642871 tig0030914:-15817 Msa1445050:CDS
TCTCCATCAACTCATTCCTG+AGG 0.645543 tig0030914:-15547 Msa1445050:CDS
AAAGTATGCTTAGATGGGTG+AGG 0.645663 tig0030914:-14599 Msa1445050:CDS
GATTGTCACAATTTCAACTG+AGG 0.649875 tig0030914:-14643 Msa1445050:CDS
GCTATGCTGATTGTGTACAT+TGG 0.671303 tig0030914:-13778 Msa1445050:CDS
GTATATTAGGAGAAAGTGGG+AGG 0.692881 tig0030914:+16555 None:intergenic
GGCAGGAGTATAGATTGGGG+AGG 0.697427 tig0030914:-14037 Msa1445050:intron
TTAGGAAGATCGCAACCGTG+TGG 0.699752 tig0030914:+15802 None:intergenic
CGGTGGGGAAGACAGAGGAA+GGG 0.705520 tig0030914:-15969 Msa1445050:CDS
GATTGGGGAGGTGAGCCAGG+AGG 0.708567 tig0030914:-14025 Msa1445050:CDS
ATTTGCGGTGGGGAAGACAG+AGG 0.711107 tig0030914:-15974 Msa1445050:CDS
GGATACACGACCATTGTACG+AGG 0.724636 tig0030914:-15911 Msa1445050:CDS
TACCCAATAAGACATCACAA+TGG 0.732110 tig0030914:+16639 None:intergenic
TTGATGCTTGAAACACTACG+TGG 0.805901 tig0030914:-15633 Msa1445050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAATAATTATATTTAG+TGG + tig0030914:14911-14930 None:intergenic 10.0%
!!! TAATTTTCAAATAATCAAAA+TGG + tig0030914:15289-15308 None:intergenic 10.0%
!! TATTATAGATCAATTTAAAT+AGG + tig0030914:15599-15618 None:intergenic 10.0%
!! ATTTAAATTGATCTATAATA+TGG - tig0030914:15599-15618 Msa1445050:CDS 10.0%
!! TTAAAATCTTATGAATATGT+CGG + tig0030914:13907-13926 None:intergenic 15.0%
!!! AATATATTATGCCTTTTTTA+TGG - tig0030914:13950-13969 Msa1445050:CDS 15.0%
!!! TTTATTTGATGTTTTGTATT+TGG - tig0030914:13973-13992 Msa1445050:CDS 15.0%
!!! TTATTTGATGTTTTGTATTT+GGG - tig0030914:13974-13993 Msa1445050:CDS 15.0%
!! TTCACTTTAATGTAATAATT+TGG - tig0030914:14774-14793 Msa1445050:intron 15.0%
!! TGAAAATCAACAATAAAAAA+TGG + tig0030914:14995-15014 None:intergenic 15.0%
!! AAGTCTAAAAAAATTGAAAA+GGG + tig0030914:15327-15346 None:intergenic 15.0%
!!! AATTGTAGAGATTTTTTTTA+AGG + tig0030914:15705-15724 None:intergenic 15.0%
!!! AAAAAAAATCTCTACAATTT+TGG - tig0030914:15706-15725 Msa1445050:intron 15.0%
!! TAAACAAAACTTTATTCTTT+AGG - tig0030914:15792-15811 Msa1445050:intron 15.0%
!! CTAATATTTCAACAAATATA+TGG - tig0030914:15958-15977 Msa1445050:CDS 15.0%
!! TGAATTATCTATATATTGTT+AGG - tig0030914:16662-16681 Msa1445050:five_prime_UTR 15.0%
!!! TTTTAACTATTAGTGTTTTT+TGG + tig0030914:16704-16723 None:intergenic 15.0%
!! AAATTAGTTATACGTATATG+AGG - tig0030914:13325-13344 Msa1445050:three_prime_UTR 20.0%
!! AATTAGTTATACGTATATGA+GGG - tig0030914:13326-13345 Msa1445050:three_prime_UTR 20.0%
!! ATTGAATGTACTAGTATATT+AGG + tig0030914:13477-13496 None:intergenic 20.0%
!!! TTTTACATCAAAAAGTATAG+AGG - tig0030914:13840-13859 Msa1445050:CDS 20.0%
!! TAAAATCTTATGAATATGTC+GGG + tig0030914:13906-13925 None:intergenic 20.0%
!! CATCAAATAAACCATAAAAA+AGG + tig0030914:13964-13983 None:intergenic 20.0%
!!! TATTTGATGTTTTGTATTTG+GGG - tig0030914:13975-13994 Msa1445050:CDS 20.0%
!! GCATAAATAAATACTTACTT+AGG + tig0030914:14235-14254 None:intergenic 20.0%
!! TTCATAACAATAATGAAGAT+CGG + tig0030914:14562-14581 None:intergenic 20.0%
!! TTCATTATTGTTATGAATGA+TGG - tig0030914:14565-14584 Msa1445050:CDS 20.0%
!! AGTTAATTATAAGAGTGAAT+CGG - tig0030914:14710-14729 Msa1445050:intron 20.0%
!! CAAATAATCAAAATGGAAAA+GGG + tig0030914:15282-15301 None:intergenic 20.0%
!! TCAAATAATCAAAATGGAAA+AGG + tig0030914:15283-15302 None:intergenic 20.0%
!! CAAGTCTAAAAAAATTGAAA+AGG + tig0030914:15328-15347 None:intergenic 20.0%
!!! CTTTTCAATTTTTTTAGACT+TGG - tig0030914:15326-15345 Msa1445050:intron 20.0%
!! ACACATTTGTTCAAATATAA+AGG - tig0030914:16331-16350 Msa1445050:CDS 20.0%
!! CACATTTGTTCAAATATAAA+GGG - tig0030914:16332-16351 Msa1445050:CDS 20.0%
!!! ACTACAAAAAATTTGGAATT+TGG + tig0030914:16597-16616 None:intergenic 20.0%
!!! TCCAAATTTTTTGTAGTTTT+TGG - tig0030914:16600-16619 Msa1445050:five_prime_UTR 20.0%
!! ACCAAAAACTACAAAAAATT+TGG + tig0030914:16604-16623 None:intergenic 20.0%
!! TAAATAATAGTGAGTTGAGT+TGG - tig0030914:13563-13582 Msa1445050:three_prime_UTR 25.0%
! AAAAAGAATGAAAGCTAGAA+GGG + tig0030914:13678-13697 None:intergenic 25.0%
!! CTTTTTGTTACTATTCTCTA+TGG - tig0030914:13692-13711 Msa1445050:three_prime_UTR 25.0%
!!! TGTAGCATTTTTTGAAACAA+TGG + tig0030914:13798-13817 None:intergenic 25.0%
! AAAATCTTATGAATATGTCG+GGG + tig0030914:13905-13924 None:intergenic 25.0%
!! ATTTGATGTTTTGTATTTGG+GGG - tig0030914:13976-13995 Msa1445050:CDS 25.0%
! TTGATAAAAGAACGAGATAA+TGG - tig0030914:14677-14696 Msa1445050:intron 25.0%
!! TGATTTTCATGTGTTTAATC+AGG - tig0030914:15006-15025 Msa1445050:intron 25.0%
!!! TTTTCTTCTAGAATCAAACT+CGG - tig0030914:15063-15082 Msa1445050:intron 25.0%
! TCTGATAGAATTGTAAGAAA+AGG - tig0030914:15109-15128 Msa1445050:intron 25.0%
!!! ATCTTTTGATGATGAAGTAA+AGG - tig0030914:15349-15368 Msa1445050:intron 25.0%
!! TATGAAAAGTATGCTTAGAT+GGG - tig0030914:15412-15431 Msa1445050:intron 25.0%
!!! GTTTTGTTTATAATAGTGAC+CGG + tig0030914:15782-15801 None:intergenic 25.0%
!! ATACGCAAAATTTCACATTT+TGG + tig0030914:15881-15900 None:intergenic 25.0%
! GAAATATTAGAACTTAGAAG+TGG + tig0030914:15948-15967 None:intergenic 25.0%
! TATTTCAACAAATATATGGC+AGG - tig0030914:15962-15981 Msa1445050:CDS 25.0%
! TGATTAATAATTCCACATAC+TGG - tig0030914:16037-16056 Msa1445050:intron 25.0%
! GATTAATAATTCCACATACT+GGG - tig0030914:16038-16057 Msa1445050:intron 25.0%
! ATTAATAATTCCACATACTG+GGG - tig0030914:16039-16058 Msa1445050:intron 25.0%
!! AAAAGTATAATGCAAGTGAT+TGG - tig0030914:16075-16094 Msa1445050:intron 25.0%
! GTGATGTTAAGAAATGTAAT+TGG + tig0030914:16126-16145 None:intergenic 25.0%
! CTAATTGCTCTTTAGTTAAA+GGG + tig0030914:16206-16225 None:intergenic 25.0%
!! ACAAATGTGTACAATTTTCA+AGG + tig0030914:16321-16340 None:intergenic 25.0%
!! ATCATATATTGTTGTTCTCT+TGG - tig0030914:16355-16374 Msa1445050:CDS 25.0%
!! TGATTAATCTTTGTTTGCAA+AGG - tig0030914:16436-16455 Msa1445050:exon 25.0%
! GAATTTGGTATTTCAGTTAT+AGG + tig0030914:16582-16601 None:intergenic 25.0%
TGAACTTATGTCTTTGTTGA+TGG + tig0030914:13429-13448 None:intergenic 30.0%
CTAGTATATTAGGAGAAAGT+GGG + tig0030914:13467-13486 None:intergenic 30.0%
ACTAGTATATTAGGAGAAAG+TGG + tig0030914:13468-13487 None:intergenic 30.0%
! GTACTTTACTCTAAAAGACT+TGG + tig0030914:13521-13540 None:intergenic 30.0%
CAAAAAGAATGAAAGCTAGA+AGG + tig0030914:13679-13698 None:intergenic 30.0%
! TTTGATGTTTTGTATTTGGG+GGG - tig0030914:13977-13996 Msa1445050:CDS 30.0%
TGATATATTCAATGGAAGGT+GGG - tig0030914:14072-14091 Msa1445050:intron 30.0%
GAAAACAGTTAGTGAATCAA+AGG + tig0030914:14513-14532 None:intergenic 30.0%
AGAAATCATAAGTATTCGTG+TGG - tig0030914:14624-14643 Msa1445050:CDS 30.0%
GAAATCATAAGTATTCGTGT+GGG - tig0030914:14625-14644 Msa1445050:CDS 30.0%
AATCAACTAGAGTTAGTTGT+TGG + tig0030914:14756-14775 None:intergenic 30.0%
ATGCAACAATTGAGTTCTAT+TGG - tig0030914:15035-15054 Msa1445050:intron 30.0%
TGCAACAATTGAGTTCTATT+GGG - tig0030914:15036-15055 Msa1445050:intron 30.0%
! GAGTTTGATTCTAGAAGAAA+AGG + tig0030914:15064-15083 None:intergenic 30.0%
AAAGGTTCAATCAATAAGCA+TGG - tig0030914:15127-15146 Msa1445050:intron 30.0%
TCAATAAGCATGGTAGAAAT+TGG - tig0030914:15137-15156 Msa1445050:intron 30.0%
TGGTATTCAACACTTATCTT+TGG - tig0030914:15176-15195 Msa1445050:intron 30.0%
TATTCAACACTTATCTTTGG+TGG - tig0030914:15179-15198 Msa1445050:intron 30.0%
GCTTGTTTGAATAGAAAAGA+TGG + tig0030914:15238-15257 None:intergenic 30.0%
! CTATGAAAAGTATGCTTAGA+TGG - tig0030914:15411-15430 Msa1445050:intron 30.0%
!!! AAGAAGACTCTAGTTTTCTT+TGG + tig0030914:15455-15474 None:intergenic 30.0%
! GAAACCAAACCATACAATTT+TGG - tig0030914:15676-15695 Msa1445050:intron 30.0%
! TTAAGGTGTCCAAAATTGTA+TGG + tig0030914:15688-15707 None:intergenic 30.0%
TGCGTATAATAATGATCAGA+AGG - tig0030914:15894-15913 Msa1445050:CDS 30.0%
CTTCAATCTATAAGAAGCAA+TGG - tig0030914:16178-16197 Msa1445050:intron 30.0%
GCTAATTGCTCTTTAGTTAA+AGG + tig0030914:16207-16226 None:intergenic 30.0%
AAGGAAGAAAGAAATGAATG+AGG - tig0030914:16476-16495 Msa1445050:five_prime_UTR 30.0%
GTTAGTAGTTGTTCAAAGAA+AGG - tig0030914:16512-16531 Msa1445050:five_prime_UTR 30.0%
TATTTCAGTTATAGGAAAGG+GGG + tig0030914:16574-16593 None:intergenic 30.0%
GTATTTCAGTTATAGGAAAG+GGG + tig0030914:16575-16594 None:intergenic 30.0%
GGTATTTCAGTTATAGGAAA+GGG + tig0030914:16576-16595 None:intergenic 30.0%
TGGTATTTCAGTTATAGGAA+AGG + tig0030914:16577-16596 None:intergenic 30.0%
ACTCCATTGTGATGTCTTAT+TGG - tig0030914:13374-13393 Msa1445050:three_prime_UTR 35.0%
! CTCCATTGTGATGTCTTATT+GGG - tig0030914:13375-13394 Msa1445050:three_prime_UTR 35.0%
TACCCAATAAGACATCACAA+TGG + tig0030914:13380-13399 None:intergenic 35.0%
! TTGTGATGTCTTATTGGGTA+AGG - tig0030914:13380-13399 Msa1445050:three_prime_UTR 35.0%
!! ATAGTGAGTTGAGTTGGATT+TGG - tig0030914:13569-13588 Msa1445050:three_prime_UTR 35.0%
TTGCTTCTTAGAATGGAAGA+AGG + tig0030914:13629-13648 None:intergenic 35.0%
! TCTTCCATTCTAAGAAGCAA+AGG - tig0030914:13629-13648 Msa1445050:three_prime_UTR 35.0%
GAAGATTTCATGTGCATCTT+TGG - tig0030914:13716-13735 Msa1445050:CDS 35.0%
GGGTGAATAATGAAAAGAGA+GGG - tig0030914:13997-14016 Msa1445050:CDS 35.0%
! GAAGGGTGTGATATATTCAA+TGG - tig0030914:14064-14083 Msa1445050:intron 35.0%
GGTGTGATATATTCAATGGA+AGG - tig0030914:14068-14087 Msa1445050:intron 35.0%
GTGATATATTCAATGGAAGG+TGG - tig0030914:14071-14090 Msa1445050:intron 35.0%
GTCAATTGAGGTTGTATGTA+CGG + tig0030914:14142-14161 None:intergenic 35.0%
!! AATTGACTTGCCAAGAACAT+GGG - tig0030914:14155-14174 Msa1445050:intron 35.0%
!! CAATGTTGGTAATCTTTGTC+GGG + tig0030914:14184-14203 None:intergenic 35.0%
!! CCAATGTTGGTAATCTTTGT+CGG + tig0030914:14185-14204 None:intergenic 35.0%
ACAAAGATTACCAACATTGG+AGG - tig0030914:14185-14204 Msa1445050:intron 35.0%
ATCAAAGGTTTCCATGGATT+TGG + tig0030914:14498-14517 None:intergenic 35.0%
TAGTGAATCAAAGGTTTCCA+TGG + tig0030914:14504-14523 None:intergenic 35.0%
! ACTAACTGTTTTCACAGCAA+AGG - tig0030914:14520-14539 Msa1445050:intron 35.0%
! ACTAGAGTTAGTTGTTGGTT+AGG + tig0030914:14751-14770 None:intergenic 35.0%
AAGCATGGTAGAAATTGGAA+AGG - tig0030914:15142-15161 Msa1445050:intron 35.0%
!!! TTGGAAAGGTGTTGACATTT+TGG - tig0030914:15156-15175 Msa1445050:intron 35.0%
GATTGTCACAATTTCAACTG+AGG - tig0030914:15373-15392 Msa1445050:intron 35.0%
TATACTCACTTTCCATGAGA+AGG + tig0030914:15491-15510 None:intergenic 35.0%
GTGTCCAAAATTGTATGGTT+TGG + tig0030914:15683-15702 None:intergenic 35.0%
! ACATGCTCGTTGTTAATTTG+AGG + tig0030914:15752-15771 None:intergenic 35.0%
TGTTTATAATAGTGACCGGA+GGG + tig0030914:15778-15797 None:intergenic 35.0%
TTGTTTATAATAGTGACCGG+AGG + tig0030914:15779-15798 None:intergenic 35.0%
! TATATGGCAGGAGTATAGAT+TGG - tig0030914:15974-15993 Msa1445050:CDS 35.0%
! ATATGGCAGGAGTATAGATT+GGG - tig0030914:15975-15994 Msa1445050:CDS 35.0%
TTGAAGTGTGTGCATCTTTA+CGG + tig0030914:16164-16183 None:intergenic 35.0%
! TACCTGGTCTTCAAGATAAT+TGG - tig0030914:16265-16284 Msa1445050:CDS 35.0%
TTCCAATTATCTTGAAGACC+AGG + tig0030914:16270-16289 None:intergenic 35.0%
! ATTGTTGTTCTCTTGGATCA+TGG - tig0030914:16362-16381 Msa1445050:CDS 35.0%
GTGACACTTTCATTTGTACA+CGG - tig0030914:16395-16414 Msa1445050:CDS 35.0%
! ACAATGGAGTTCCCTCTTTT+GGG + tig0030914:13364-13383 None:intergenic 40.0%
GTATATTAGGAGAAAGTGGG+AGG + tig0030914:13464-13483 None:intergenic 40.0%
AGAATGGAAGAAGGTGAGAA+TGG + tig0030914:13620-13639 None:intergenic 40.0%
ACGACCTTTGCTTCTTAGAA+TGG + tig0030914:13636-13655 None:intergenic 40.0%
TAGAAGGGTGAAAAGGTAAG+GGG + tig0030914:13663-13682 None:intergenic 40.0%
CTAGAAGGGTGAAAAGGTAA+GGG + tig0030914:13664-13683 None:intergenic 40.0%
TGAAAGCTAGAAGGGTGAAA+AGG + tig0030914:13670-13689 None:intergenic 40.0%
GTGTGGAGAAGAATTTGTCT+TGG + tig0030914:13766-13785 None:intergenic 40.0%
! TTTGAAACAATGGACTCACC+AGG + tig0030914:13788-13807 None:intergenic 40.0%
GCGAGTTTGAACTCTCTTAT+GGG + tig0030914:13881-13900 None:intergenic 40.0%
GGGGTGAATAATGAAAAGAG+AGG - tig0030914:13996-14015 Msa1445050:CDS 40.0%
! TATTCAATGGAAGGTGGGTT+TGG - tig0030914:14077-14096 Msa1445050:intron 40.0%
! ATTCAATGGAAGGTGGGTTT+GGG - tig0030914:14078-14097 Msa1445050:intron 40.0%
TGTTCTTGGCAAGTCAATTG+AGG + tig0030914:14154-14173 None:intergenic 40.0%
!! CAATTGACTTGCCAAGAACA+TGG - tig0030914:14154-14173 Msa1445050:intron 40.0%
CCGACAAAGATTACCAACAT+TGG - tig0030914:14182-14201 Msa1445050:intron 40.0%
AAGATTACCAACATTGGAGG+TGG - tig0030914:14188-14207 Msa1445050:intron 40.0%
TTGATGCTTGAAACACTACG+TGG - tig0030914:14383-14402 Msa1445050:intron 40.0%
TTGAAACACTACGTGGAAAG+AGG - tig0030914:14390-14409 Msa1445050:intron 40.0%
GGAAAGAGGATGATGTTTGT+TGG - tig0030914:14404-14423 Msa1445050:intron 40.0%
TAGAGATCATAGTGATCAGC+AGG - tig0030914:14599-14618 Msa1445050:CDS 40.0%
! ACTTATCTTTGGTGGATGAC+TGG - tig0030914:15187-15206 Msa1445050:intron 40.0%
AACTGAGGATGCTTATCGTA+TGG - tig0030914:15388-15407 Msa1445050:intron 40.0%
AAAGTATGCTTAGATGGGTG+AGG - tig0030914:15417-15436 Msa1445050:intron 40.0%
! TATGGCAGGAGTATAGATTG+GGG - tig0030914:15976-15995 Msa1445050:CDS 40.0%
TCATTGTAACAACTTCCTCC+TGG + tig0030914:16009-16028 None:intergenic 40.0%
CACAATCAGCATAGCTTACA+GGG + tig0030914:16233-16252 None:intergenic 40.0%
ACACAATCAGCATAGCTTAC+AGG + tig0030914:16234-16253 None:intergenic 40.0%
! GCTATGCTGATTGTGTACAT+TGG - tig0030914:16238-16257 Msa1445050:intron 40.0%
! TGTGTACATTGGTGTTTACC+TGG - tig0030914:16249-16268 Msa1445050:intron 40.0%
! CACGGAAAAGAGTATAGCTA+CGG - tig0030914:16413-16432 Msa1445050:CDS 40.0%
GGATACATGTGACGATAACA+AGG - tig0030914:16457-16476 Msa1445050:five_prime_UTR 40.0%
ACCACTTAAGTGCCCAAAAG+AGG - tig0030914:13349-13368 Msa1445050:three_prime_UTR 45.0%
CCACTTAAGTGCCCAAAAGA+GGG - tig0030914:13350-13369 Msa1445050:three_prime_UTR 45.0%
! CACAATGGAGTTCCCTCTTT+TGG + tig0030914:13365-13384 None:intergenic 45.0%
ATGGAAGAAGGTGAGAATGG+TGG + tig0030914:13617-13636 None:intergenic 45.0%
GCTAGAAGGGTGAAAAGGTA+AGG + tig0030914:13665-13684 None:intergenic 45.0%
GACAAATTCTTCTCCACACC+TGG - tig0030914:13767-13786 Msa1445050:CDS 45.0%
CGCGAGTTTGAACTCTCTTA+TGG + tig0030914:13882-13901 None:intergenic 45.0%
ACGACCATTGTACGAGGAAT+CGG - tig0030914:14111-14130 Msa1445050:intron 45.0%
!! CAGGAATGAGTTGATGGAGA+AGG + tig0030914:14469-14488 None:intergenic 45.0%
TCTCCATCAACTCATTCCTG+AGG - tig0030914:14469-14488 Msa1445050:intron 45.0%
! CATCCTCAGGAATGAGTTGA+TGG + tig0030914:14475-14494 None:intergenic 45.0%
CGGACAATGCTGTTGTTCAT+AGG - tig0030914:15083-15102 Msa1445050:intron 45.0%
ACTAGCATGTCACCTTCTCA+TGG - tig0030914:15476-15495 Msa1445050:intron 45.0%
ACAATCAGAACCCCAGTATG+TGG + tig0030914:16052-16071 None:intergenic 45.0%
TCTCTTGGATCATGGACTCT+TGG - tig0030914:16370-16389 Msa1445050:CDS 45.0%
CTCTTGGATCATGGACTCTT+GGG - tig0030914:16371-16390 Msa1445050:CDS 45.0%
! CCCTCTTTTGGGCACTTAAG+TGG + tig0030914:13353-13372 None:intergenic 50.0%
! AACAATGGACTCACCAGGTG+TGG + tig0030914:13783-13802 None:intergenic 50.0%
GAGGGTGAAGAAGCAGATCA+AGG - tig0030914:14015-14034 Msa1445050:CDS 50.0%
!! GCAGATCAAGGTGCCATTTG+CGG - tig0030914:14027-14046 Msa1445050:CDS 50.0%
!! ATCAAGGTGCCATTTGCGGT+GGG - tig0030914:14031-14050 Msa1445050:CDS 50.0%
GGATACACGACCATTGTACG+AGG - tig0030914:14105-14124 Msa1445050:intron 50.0%
CACTCCGATTCCTCGTACAA+TGG + tig0030914:14118-14137 None:intergenic 50.0%
TTAGGAAGATCGCAACCGTG+TGG + tig0030914:14217-14236 None:intergenic 50.0%
GTTGGAGACTCTCTCAATCG+TGG - tig0030914:14422-14441 Msa1445050:intron 50.0%
TCCTGAGGATGCCAAATCCA+TGG - tig0030914:14484-14503 Msa1445050:intron 50.0%
TCCATGGATTTGGCATCCTC+AGG + tig0030914:14488-14507 None:intergenic 50.0%
GTTAACACGCGGAGGATACT+TGG + tig0030914:14806-14825 None:intergenic 50.0%
CGAGCAACAACGTTAACACG+CGG + tig0030914:14817-14836 None:intergenic 50.0%
! TCTTTGGTGGATGACTGGCT+TGG - tig0030914:15192-15211 Msa1445050:intron 50.0%
!! GATCAAGGTGCCATTTGCGG+TGG - tig0030914:14030-14049 Msa1445050:CDS 55.0%
!! TCAAGGTGCCATTTGCGGTG+GGG - tig0030914:14032-14051 Msa1445050:CDS 55.0%
TCTGTCTTCCCCACCGCAAA+TGG + tig0030914:14043-14062 None:intergenic 55.0%
ATTTGCGGTGGGGAAGACAG+AGG - tig0030914:14042-14061 Msa1445050:intron 55.0%
TGTCGGGTCTCCCATGTTCT+TGG + tig0030914:14168-14187 None:intergenic 55.0%
GTGGTTGCCACCTCCAATGT+TGG + tig0030914:14198-14217 None:intergenic 55.0%
CATTGGAGGTGGCAACCACA+CGG - tig0030914:14199-14218 Msa1445050:intron 55.0%
GCAACAACGTTAACACGCGG+AGG + tig0030914:14814-14833 None:intergenic 55.0%
CAACGAGCATGTACACCCTC+CGG - tig0030914:15760-15779 Msa1445050:intron 55.0%
! GGCAGGAGTATAGATTGGGG+AGG - tig0030914:15979-15998 Msa1445050:CDS 55.0%
ATAGATTGGGGAGGTGAGCC+AGG - tig0030914:15988-16007 Msa1445050:CDS 55.0%
CGGTGGGGAAGACAGAGGAA+GGG - tig0030914:14047-14066 Msa1445050:intron 60.0%
! TGGAAGGTGGGTTTGGGACG+AGG - tig0030914:14084-14103 Msa1445050:intron 60.0%
GCGGTGGGGAAGACAGAGGA+AGG - tig0030914:14046-14065 Msa1445050:intron 65.0%
GATTGGGGAGGTGAGCCAGG+AGG - tig0030914:15991-16010 Msa1445050:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0030914 gene 13310 16728 13310 ID=Msa1445050;Name=Msa1445050
tig0030914 mRNA 13310 16728 13310 ID=Msa1445050-mRNA-1;Parent=Msa1445050;Name=Msa1445050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=289|1|1|1|1|1|6|405|427
tig0030914 exon 13310 14054 13310 ID=Msa1445050-mRNA-1:exon:14800;Parent=Msa1445050-mRNA-1
tig0030914 exon 14537 14695 14537 ID=Msa1445050-mRNA-1:exon:14799;Parent=Msa1445050-mRNA-1
tig0030914 exon 14811 15010 14811 ID=Msa1445050-mRNA-1:exon:14798;Parent=Msa1445050-mRNA-1
tig0030914 exon 15497 15668 15497 ID=Msa1445050-mRNA-1:exon:14797;Parent=Msa1445050-mRNA-1
tig0030914 exon 15802 16024 15802 ID=Msa1445050-mRNA-1:exon:14796;Parent=Msa1445050-mRNA-1
tig0030914 exon 16250 16728 16250 ID=Msa1445050-mRNA-1:exon:14795;Parent=Msa1445050-mRNA-1
tig0030914 five_prime_UTR 16440 16728 16440 ID=Msa1445050-mRNA-1:five_prime_utr;Parent=Msa1445050-mRNA-1
tig0030914 CDS 16250 16439 16250 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 CDS 15802 16024 15802 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 CDS 15497 15668 15497 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 CDS 14811 15010 14811 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 CDS 14537 14695 14537 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 CDS 13715 14054 13715 ID=Msa1445050-mRNA-1:cds;Parent=Msa1445050-mRNA-1
tig0030914 three_prime_UTR 13310 13714 13310 ID=Msa1445050-mRNA-1:three_prime_utr;Parent=Msa1445050-mRNA-1
Gene Sequence

>Msa1445050

ATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCATTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGAGTATAGATTGGGGAGGTGAGCCAGGAGGAAGTTGTTACAATGAAACAACTTTGATTAATAATTCCACATACTGGGGTTCTGATTGTAGAAAAAGTATAATGCAAGTGATTGGTGAAGTGTTAAGCAAAACAAAAGTTCCAATTACATTTCTTAACATCACACAACTCTCAAGTTACCGTAAAGATGCACACACTTCAATCTATAAGAAGCAATGGAGCCCTTTAACTAAAGAGCAATTAGCTAACCCTGTAAGCTATGCTGATTGTGTACATTGGTGTTTACCTGGTCTTCAAGATAATTGGAATGAACTTTTGTTTGCTAAGTTGTTTTATCCTTGA
Protein sequence

>Msa1445050

MQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGKSIDWGGEPGGSCYNETTLINNSTYWGSDCRKSIMQVIGEVLSKTKVPITFLNITQLSSYRKDAHTSIYKKQWSPLTKEQLANPVSYADCVHWCLPGLQDNWNELLFAKLFYP*