AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732410 MtrunA17_Chr5g0437601 99.297 427 3 0 1 427 1 427 0.0 890
Msa0732410 MtrunA17_Chr3g0114101 74.596 433 101 5 4 427 1 433 0.0 660
Msa0732410 MtrunA17_Chr4g0005491 51.003 349 165 3 83 426 62 409 2.44e-132 387
Msa0732410 MtrunA17_Chr7g0243301 48.677 378 183 5 53 425 39 410 4.74e-132 386
Msa0732410 MtrunA17_Chr4g0005481 52.187 343 161 2 79 421 104 443 3.05e-129 380
Msa0732410 MtrunA17_Chr4g0006991 48.986 345 174 2 81 424 84 427 9.86e-129 378
Msa0732410 MtrunA17_Chr8g0360521 49.025 359 175 4 71 425 56 410 5.42e-128 376
Msa0732410 MtrunA17_Chr3g0113671 51.289 349 164 4 80 424 103 449 1.25e-126 374
Msa0732410 MtrunA17_Chr5g0437291 48.571 350 175 3 81 425 163 512 9.06e-119 356
Msa0732410 MtrunA17_Chr7g0242701 41.647 425 238 4 10 424 3 427 1.09e-118 352
Msa0732410 MtrunA17_Chr5g0430371 36.675 379 207 9 64 426 40 401 1.24e-76 244
Msa0732410 MtrunA17_Chr3g0105301 36.944 360 201 8 76 426 36 378 1.57e-76 243
Msa0732410 MtrunA17_Chr5g0413271 36.932 352 206 8 77 425 139 477 2.08e-75 243
Msa0732410 MtrunA17_Chr4g0041011 34.097 393 226 9 32 424 4 363 3.49e-75 239
Msa0732410 MtrunA17_Chr1g0210771 38.329 347 186 10 83 424 59 382 1.11e-74 238
Msa0732410 MtrunA17_Chr2g0325481 37.026 343 191 7 83 425 50 367 2.62e-74 236
Msa0732410 MtrunA17_Chr1g0175671 36.723 354 200 8 83 425 170 510 1.90e-73 239
Msa0732410 MtrunA17_Chr3g0097891 35.127 353 203 8 75 425 23 351 1.39e-72 231
Msa0732410 MtrunA17_Chr7g0258851 37.255 357 194 9 83 425 248 588 1.79e-72 238
Msa0732410 MtrunA17_Chr1g0171171 33.668 398 234 10 27 424 8 375 6.79e-72 231
Msa0732410 MtrunA17_Chr7g0259711 33.034 445 258 15 2 425 8 433 1.59e-71 232
Msa0732410 MtrunA17_Chr4g0071391 37.143 350 200 8 83 424 109 446 2.05e-71 232
Msa0732410 MtrunA17_Chr2g0287901 37.183 355 196 11 81 422 95 435 5.72e-70 228
Msa0732410 MtrunA17_Chr2g0314801 36.286 350 192 8 77 421 27 350 9.66e-69 222
Msa0732410 MtrunA17_Chr2g0283581 34.925 398 211 11 27 424 9 358 2.57e-68 221
Msa0732410 MtrunA17_Chr7g0269991 36.648 352 192 9 83 424 137 467 5.71e-68 223
Msa0732410 MtrunA17_Chr3g0085081 35.817 349 190 10 81 424 29 348 1.29e-67 219
Msa0732410 MtrunA17_Chr5g0413851 37.644 348 189 10 79 424 38 359 1.54e-67 219
Msa0732410 MtrunA17_Chr4g0045941 33.903 351 208 8 75 425 25 351 1.55e-66 216
Msa0732410 MtrunA17_Chr3g0085071 35.817 349 190 10 81 424 44 363 2.31e-66 216
Msa0732410 MtrunA17_Chr1g0213281 33.943 383 231 10 56 426 145 517 2.39e-66 220
Msa0732410 MtrunA17_Chr4g0068111 33.711 353 212 7 83 425 114 454 7.10e-66 218
Msa0732410 MtrunA17_Chr3g0091031 35.244 349 192 10 81 424 29 348 2.71e-65 213
Msa0732410 MtrunA17_Chr8g0368461 33.333 372 213 10 76 425 171 529 3.28e-63 213
Msa0732410 MtrunA17_Chr4g0045951 33.333 348 208 8 78 425 40 363 1.50e-61 203
Msa0732410 MtrunA17_Chr2g0314791 35.393 356 185 13 80 425 26 346 2.01e-60 200
Msa0732410 MtrunA17_Chr8g0384151 28.440 436 260 13 5 425 19 417 6.83e-55 188
Msa0732410 MtrunA17_Chr3g0126021 29.609 358 219 8 79 425 125 460 2.06e-52 182
Msa0732410 MtrunA17_Chr6g0456681 30.833 360 220 10 81 424 62 408 3.83e-43 156
Msa0732410 MtrunA17_Chr1g0209921 34.043 235 143 6 81 313 42 266 3.91e-42 149
Msa0732410 MtrunA17_Chr1g0188191 27.889 398 254 16 43 421 195 578 4.66e-40 150
Msa0732410 MtrunA17_Chr1g0178081 29.261 352 180 9 81 425 53 342 5.44e-40 146
Msa0732410 MtrunA17_Chr6g0456691 27.419 434 262 16 16 424 14 419 3.69e-39 145
Msa0732410 MtrunA17_Chr8g0347321 28.571 357 228 12 83 424 95 439 6.88e-39 145
Msa0732410 MtrunA17_Chr8g0384421 30.028 363 214 13 81 424 131 472 2.46e-37 141
Msa0732410 MtrunA17_Chr6g0476041 28.011 357 231 14 81 421 165 511 3.29e-37 142
Msa0732410 MtrunA17_Chr8g0391481 29.428 367 219 15 80 424 24 372 2.38e-36 137
Msa0732410 MtrunA17_Chr6g0451321 28.032 371 229 14 73 424 106 457 5.76e-36 137
Msa0732410 MtrunA17_Chr5g0414061 27.887 355 227 12 83 421 94 435 4.22e-35 135
Msa0732410 MtrunA17_Chr5g0414091 28.453 362 222 12 80 420 82 427 8.64e-35 134
Msa0732410 MtrunA17_Chr2g0283851 27.559 381 230 15 65 420 37 396 8.87e-35 133
Msa0732410 MtrunA17_Chr5g0414051 25.886 367 226 13 80 421 72 417 4.38e-33 129
Msa0732410 MtrunA17_Chr3g0140371 28.333 360 223 13 80 421 15 357 9.76e-33 127
Msa0732410 MtrunA17_Chr7g0226991 27.348 362 227 14 80 421 54 399 1.43e-32 127
Msa0732410 MtrunA17_Chr8g0387921 26.479 355 231 12 83 420 74 415 2.71e-32 127
Msa0732410 MtrunA17_Chr5g0414041 28.571 357 224 12 83 421 87 430 1.44e-31 125
Msa0732410 MtrunA17_Chr4g0049421 26.997 363 218 16 83 420 48 388 1.69e-29 118
Msa0732410 MtrunA17_Chr6g0455901 26.503 366 231 12 80 424 68 416 9.03e-29 117
Msa0732410 MtrunA17_Chr4g0070341 27.704 379 202 16 81 424 130 471 2.22e-27 113
Msa0732410 MtrunA17_Chr7g0221271 40.000 130 77 1 81 210 77 205 4.19e-26 104
Msa0732410 MtrunA17_Chr7g0221291 45.361 97 52 1 79 175 75 170 1.71e-24 99.4
Msa0732410 MtrunA17_Chr2g0287931 36.134 119 66 4 143 252 1 118 1.77e-15 72.8
Msa0732410 MtrunA17_Chr3g0085101 38.318 107 62 3 280 385 5 108 6.20e-13 65.5
Msa0732410 MtrunA17_Chr7g0221281 25.311 241 155 10 197 424 8 236 7.06e-12 65.5
Msa0732410 MtrunA17_Chr2g0287911 43.750 64 28 2 76 139 54 109 1.23e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732410 AT2G40320.1 78.537 410 86 1 17 426 17 424 0.0 689
Msa0732410 AT3G11030.1 62.723 448 137 4 8 427 6 451 0.0 588
Msa0732410 AT5G01620.2 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa0732410 AT5G01620.1 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa0732410 AT5G01620.3 50.000 350 171 2 73 421 108 454 1.00e-128 379
Msa0732410 AT3G55990.1 53.009 349 158 3 81 425 138 484 4.94e-128 379
Msa0732410 AT1G73140.1 44.444 378 193 3 49 425 45 406 7.02e-127 373
Msa0732410 AT5G01360.2 48.696 345 176 1 81 424 34 378 1.68e-126 371
Msa0732410 AT2G40150.1 51.233 365 163 4 73 424 58 420 5.92e-126 371
Msa0732410 AT5G01360.1 48.696 345 176 1 81 424 90 434 6.81e-126 371
Msa0732410 AT2G38320.1 44.865 370 198 3 61 425 37 405 8.04e-117 347
Msa0732410 AT2G40160.1 40.821 414 229 7 23 426 18 425 1.70e-107 324
Msa0732410 AT2G40160.2 41.176 408 224 7 23 420 18 419 4.32e-107 323
Msa0732410 AT2G38320.2 47.188 320 163 3 111 425 4 322 1.18e-105 316
Msa0732410 AT3G12060.1 38.259 379 204 9 61 425 174 536 3.80e-82 263
Msa0732410 AT5G06700.1 38.547 358 192 10 81 425 253 595 1.11e-78 255
Msa0732410 AT3G62390.1 37.166 374 203 11 68 426 117 473 1.97e-75 243
Msa0732410 AT1G78710.1 35.429 350 199 8 78 425 33 357 3.19e-75 239
Msa0732410 AT5G20590.1 36.639 363 209 8 72 425 130 480 3.68e-74 240
Msa0732410 AT2G40160.3 39.799 299 166 5 23 313 18 310 3.78e-74 234
Msa0732410 AT5G19160.1 34.651 430 237 14 20 422 30 442 5.21e-74 239
Msa0732410 AT3G14850.2 37.249 349 188 7 78 421 29 351 9.90e-73 233
Msa0732410 AT2G30900.1 35.135 370 204 12 68 426 23 367 4.13e-72 231
Msa0732410 AT3G06080.2 32.118 439 247 12 15 422 25 443 1.82e-70 230
Msa0732410 AT5G58600.1 35.411 353 203 7 83 426 66 402 2.22e-70 228
Msa0732410 AT5G49340.1 35.230 369 219 9 68 425 83 442 2.39e-70 229
Msa0732410 AT1G29050.1 34.582 347 203 6 78 424 56 378 4.46e-70 226
Msa0732410 AT2G30010.1 35.309 388 214 10 54 426 33 398 5.49e-70 227
Msa0732410 AT2G42570.1 34.140 372 217 9 54 425 22 365 1.17e-69 225
Msa0732410 AT2G30010.2 35.309 388 214 10 54 426 76 441 1.61e-69 227
Msa0732410 AT2G34070.1 34.473 351 199 8 78 424 60 383 2.36e-69 224
Msa0732410 AT2G31110.2 36.047 344 192 9 83 424 45 362 7.11e-69 223
Msa0732410 AT1G60790.1 34.551 356 212 9 81 425 188 533 2.02e-68 226
Msa0732410 AT3G54260.1 36.578 339 195 8 83 420 51 370 8.23e-67 218
Msa0732410 AT5G06230.2 34.426 366 196 11 83 425 21 365 3.10e-61 203
Msa0732410 AT5G06230.1 34.426 366 196 11 83 425 62 406 1.14e-60 203
Msa0732410 AT1G48880.1 30.095 422 232 14 23 425 63 440 4.01e-57 194
Msa0732410 AT1G78710.2 30.747 348 165 8 78 425 26 297 2.00e-55 186
Msa0732410 AT5G19160.2 34.505 313 186 8 20 317 30 338 1.45e-54 185
Msa0732410 AT3G11570.1 33.828 337 198 7 100 425 98 420 2.34e-54 186
Msa0732410 AT3G62390.2 38.113 265 143 8 68 317 117 375 9.30e-52 179
Msa0732410 AT3G06080.1 35.743 249 146 5 76 313 93 338 6.06e-50 172
Msa0732410 AT5G20590.2 37.450 251 144 5 72 313 130 376 1.88e-49 172
Msa0732410 AT2G30900.2 35.271 258 145 8 68 315 23 268 8.85e-47 162
Msa0732410 AT2G31110.3 35.088 228 135 5 83 308 45 261 1.98e-44 156
Msa0732410 AT1G70230.1 29.609 358 221 11 78 424 74 411 2.07e-43 157
Msa0732410 AT5G58600.2 33.473 239 140 5 83 315 66 291 1.16e-42 152
Msa0732410 AT2G34070.2 32.364 275 156 7 154 424 1 249 2.64e-42 150
Msa0732410 AT3G14850.1 34.066 273 150 6 154 421 1 248 4.96e-42 149
Msa0732410 AT3G28150.1 28.000 425 245 16 18 422 25 408 1.89e-41 152
Msa0732410 AT5G06230.3 34.524 252 134 7 83 313 62 303 9.25e-41 147
Msa0732410 AT5G15900.1 29.082 392 247 12 50 424 32 409 4.75e-40 148
Msa0732410 AT5G15900.2 29.082 392 247 12 50 424 51 428 5.81e-40 148
Msa0732410 AT5G15890.1 28.652 356 220 10 80 421 184 519 3.14e-38 145
Msa0732410 AT5G64020.1 29.301 372 213 16 83 424 57 408 1.11e-37 141
Msa0732410 AT5G64470.3 29.915 351 218 13 83 420 55 390 3.09e-37 140
Msa0732410 AT3G02440.1 26.966 356 232 11 83 424 127 468 7.38e-37 140
Msa0732410 AT3G02440.2 26.966 356 232 11 83 424 135 476 1.03e-36 140
Msa0732410 AT5G64470.2 29.494 356 219 12 83 420 55 396 2.05e-36 138
Msa0732410 AT1G01430.1 29.661 354 224 12 83 420 99 443 1.81e-35 136
Msa0732410 AT4G25360.1 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa0732410 AT4G25360.2 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa0732410 AT4G23790.1 25.059 427 276 17 21 424 22 427 8.86e-35 134
Msa0732410 AT5G51640.1 27.174 368 225 15 80 421 135 485 4.38e-34 133
Msa0732410 AT2G37720.1 28.571 385 218 17 66 424 129 482 5.56e-34 132
Msa0732410 AT4G01080.1 29.429 350 221 14 83 420 91 426 1.05e-33 131
Msa0732410 AT2G14530.1 26.519 362 229 10 83 424 62 406 1.17e-33 130
Msa0732410 AT2G31110.1 33.621 232 127 8 195 424 8 214 6.24e-31 118
Msa0732410 AT5G20680.6 26.178 382 208 16 79 424 114 457 1.06e-29 120
Msa0732410 AT5G20680.2 26.178 382 208 16 79 424 190 533 1.77e-29 120
Msa0732410 AT5G20680.4 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0732410 AT5G20680.1 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0732410 AT5G20680.3 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0732410 AT5G20680.5 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0732410 AT4G11090.1 23.040 421 288 15 23 421 16 422 7.15e-27 112
Msa0732410 AT5G64470.1 27.985 268 178 9 83 340 55 317 3.72e-23 99.8
Msa0732410 AT5G64470.4 27.715 267 178 9 83 339 55 316 1.27e-22 98.2

Find 127 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTGGTCTTCAAGATAAT+TGG 0.191574 5_1:+44812908 Msa0732410:CDS
TTGGAAAGGTGTTGACATTT+TGG 0.194283 5_1:+44811799 Msa0732410:CDS
GCTAATTGCTCTTTAGTTAA+AGG 0.201296 5_1:-44812847 None:intergenic
ACACATTTGTTCAAATATAA+AGG 0.225938 5_1:+44812974 Msa0732410:three_prime_UTR
TGATTAATAATTCCACATAC+TGG 0.251655 5_1:+44812680 Msa0732410:CDS
TGTCGGGTCTCCCATGTTCT+TGG 0.253030 5_1:-44810808 None:intergenic
ATGCAACAATTGAGTTCTAT+TGG 0.254941 5_1:+44811678 Msa0732410:CDS
GAATTTGGTATTTCAGTTAT+AGG 0.257936 5_1:-44813226 None:intergenic
TCAATAAGCATGGTAGAAAT+TGG 0.262180 5_1:+44811780 Msa0732410:CDS
TTGAAGTGTGTGCATCTTTA+CGG 0.287806 5_1:-44812804 None:intergenic
TGAATTATCTATATATTGTT+AGG 0.290624 5_1:+44813309 Msa0732410:three_prime_UTR
ATCAAAGGTTTCCATGGATT+TGG 0.299044 5_1:-44811138 None:intergenic
ATCATATATTGTTGTTCTCT+TGG 0.308084 5_1:+44812998 Msa0732410:three_prime_UTR
TTGTGATGTCTTATTGTATA+AGG 0.308116 5_1:+44809959 Msa0732410:five_prime_UTR
GAATGTACTAGTAGTATATT+AGG 0.312483 5_1:-44810114 None:intergenic
CTAATTGCTCTTTAGTTAAA+GGG 0.317364 5_1:-44812846 None:intergenic
GAAGATTTCATGTGCATCTT+TGG 0.320970 5_1:+44810359 Msa0732410:CDS
TATTCAATGGAAGGTGGGTT+TGG 0.326490 5_1:+44810720 Msa0732410:CDS
TGAACTTATGTCTTTGTTGA+TGG 0.335672 5_1:-44810032 None:intergenic
GTGATGTTAAGAAATGTAAT+TGG 0.337450 5_1:-44812766 None:intergenic
TGGTATTCAACACTTATCTT+TGG 0.343420 5_1:+44811819 Msa0732410:CDS
TCTCTTGGATCATGGACTCT+TGG 0.348285 5_1:+44813013 Msa0732410:three_prime_UTR
ACACAATCAGCATAGCTTAC+AGG 0.357812 5_1:-44812874 None:intergenic
CACATTTGTTCAAATATAAA+GGG 0.368100 5_1:+44812975 Msa0732410:three_prime_UTR
ATTCAATGGAAGGTGGGTTT+GGG 0.383497 5_1:+44810721 Msa0732410:CDS
CGGACAATGCTGTTGTTCAT+AGG 0.387335 5_1:+44811726 Msa0732410:CDS
CTCTTGGATCATGGACTCTT+GGG 0.388166 5_1:+44813014 Msa0732410:three_prime_UTR
ATTGTTGTTCTCTTGGATCA+TGG 0.391390 5_1:+44813005 Msa0732410:three_prime_UTR
ACGACCTTTGCTTCTTAGAA+TGG 0.393423 5_1:-44810276 None:intergenic
CCAATGTTGGTAATCTTTGT+CGG 0.403476 5_1:-44810825 None:intergenic
CTATGAAAAGTATGCTTAGA+TGG 0.410597 5_1:+44812054 Msa0732410:CDS
TCTGTCTTCCCCACCGCAAA+TGG 0.411531 5_1:-44810683 None:intergenic
TGCAACAATTGAGTTCTATT+GGG 0.419007 5_1:+44811679 Msa0732410:CDS
GGTATTTCAGTTATAGGAAA+GGG 0.419698 5_1:-44813220 None:intergenic
TATTCAACACTTATCTTTGG+TGG 0.424745 5_1:+44811822 Msa0732410:CDS
TCCATGGATTTGGCATCCTC+AGG 0.427436 5_1:-44811128 None:intergenic
ACTTATCTTTGGTGGATGAC+TGG 0.435500 5_1:+44811830 Msa0732410:CDS
ATATGGCAGGAGTATAGATT+GGG 0.440954 5_1:+44812618 Msa0732410:intron
TATATGGCAGGAGTATAGAT+TGG 0.441176 5_1:+44812617 Msa0732410:intron
TAAATAATAGTGAGTTGAGT+TGG 0.441530 5_1:+44810206 Msa0732410:five_prime_UTR
ATAGATTGGGGAGGTGAGCC+AGG 0.443479 5_1:+44812631 Msa0732410:CDS
GTTAGTAGTTGTTCAAAGAA+AGG 0.448939 5_1:+44813159 Msa0732410:three_prime_UTR
ATAGTGAGTTGAGTTGGATT+TGG 0.450733 5_1:+44810212 Msa0732410:five_prime_UTR
CATCCTCAGGAATGAGTTGA+TGG 0.450737 5_1:-44811115 None:intergenic
CAATGTTGGTAATCTTTGTC+GGG 0.457780 5_1:-44810824 None:intergenic
GCTAGAAGGGTGAAAAGGTA+AGG 0.463485 5_1:-44810305 None:intergenic
TGAAAGCTAGAAGGGTGAAA+AGG 0.463624 5_1:-44810310 None:intergenic
GATTAATAATTCCACATACT+GGG 0.472858 5_1:+44812681 Msa0732410:CDS
TGTGTACATTGGTGTTTACC+TGG 0.477328 5_1:+44812892 Msa0732410:CDS
TGGAAGGTGGGTTTGGGACG+AGG 0.477341 5_1:+44810727 Msa0732410:CDS
CACAATCAGCATAGCTTACA+GGG 0.477761 5_1:-44812873 None:intergenic
AAAAGTATAATGCAAGTGAT+TGG 0.480921 5_1:+44812718 Msa0732410:CDS
TCTGATAGAATTGTAAGAAA+AGG 0.484458 5_1:+44811752 Msa0732410:CDS
ACGACCACTGTACGAGGAAT+CGG 0.484764 5_1:+44810754 Msa0732410:CDS
AGTAGTATATTAGGAGAAAG+TGG 0.490847 5_1:-44810105 None:intergenic
TGATTAATCTTTGTTTGCAA+AGG 0.491547 5_1:+44813079 Msa0732410:three_prime_UTR
TAGTGAATCAAAGGTTTCCA+TGG 0.492850 5_1:-44811144 None:intergenic
GGAAAGAGGATGATGTTTGT+TGG 0.493089 5_1:+44811047 Msa0732410:CDS
GCAGATCAAGGTGCCATTTG+CGG 0.494304 5_1:+44810670 Msa0732410:CDS
GAAGGGTGTGATATATTCAA+TGG 0.494550 5_1:+44810707 Msa0732410:CDS
GTGATATATTCAATGGAAGG+TGG 0.495098 5_1:+44810714 Msa0732410:CDS
TCTTTGGTGGATGACTGGCT+TGG 0.496770 5_1:+44811835 Msa0732410:CDS
TCATTGTAACAACTTCCTCC+TGG 0.501058 5_1:-44812649 None:intergenic
GAGTTTGATTCTAGAAGAAA+AGG 0.501756 5_1:-44811704 None:intergenic
TGTTCTTGGCAAGTCAATTG+AGG 0.502642 5_1:-44810794 None:intergenic
AACTGAGGATGCTTATCGTA+TGG 0.502726 5_1:+44812031 Msa0732410:CDS
CAATTGACTTGCCAAGAACA+TGG 0.509598 5_1:+44810797 Msa0732410:CDS
GTCAATTGAGGTTGTATGTA+CGG 0.512274 5_1:-44810782 None:intergenic
GACAAATTCTTCTCCACACC+TGG 0.515054 5_1:+44810410 Msa0732410:CDS
ACTAGCATGTCACCTTCTCA+TGG 0.524451 5_1:+44812119 Msa0732410:CDS
TTCCAATTATCTTGAAGACC+AGG 0.528264 5_1:-44812910 None:intergenic
GAAAACAGTTAGTGAATCAA+AGG 0.528871 5_1:-44811153 None:intergenic
TGATATATTCAATGGAAGGT+GGG 0.529302 5_1:+44810715 Msa0732410:CDS
CTAGAAGGGTGAAAAGGTAA+GGG 0.530481 5_1:-44810304 None:intergenic
AGAATGGAAGAAGGTGAGAA+TGG 0.531203 5_1:-44810260 None:intergenic
GCGGTGGGGAAGACAGAGGA+AGG 0.532473 5_1:+44810689 Msa0732410:CDS
AAGAAAGAAATGAACGAATG+AGG 0.539820 5_1:+44813123 Msa0732410:three_prime_UTR
ACAATCAGAACCCCAGTATG+TGG 0.541987 5_1:-44812692 None:intergenic
TTGCTTCTTAGAATGGAAGA+AGG 0.542128 5_1:-44810269 None:intergenic
CTTCAATCTATAAGAAGCAA+TGG 0.542329 5_1:+44812821 Msa0732410:CDS
TGGTATTTCAGTTATAGGAA+AGG 0.542790 5_1:-44813221 None:intergenic
TCCTGAGGATGCCAAATCCA+TGG 0.545590 5_1:+44811127 Msa0732410:CDS
GTACTTTACTCTAAAAGACT+TGG 0.546625 5_1:-44810161 None:intergenic
GAGGGTGAAGAAGCAGATCA+AGG 0.550519 5_1:+44810658 Msa0732410:CDS
TATACTCACTTTCCATGAGA+AGG 0.556322 5_1:-44812131 None:intergenic
ATCAAGGTGCCATTTGCGGT+GGG 0.557278 5_1:+44810674 Msa0732410:CDS
GGTGTGATATATTCAATGGA+AGG 0.558509 5_1:+44810711 Msa0732410:CDS
CACTCCGATTCCTCGTACAG+TGG 0.561364 5_1:-44810758 None:intergenic
TAGAAGGGTGAAAAGGTAAG+GGG 0.565912 5_1:-44810303 None:intergenic
GATCAAGGTGCCATTTGCGG+TGG 0.568008 5_1:+44810673 Msa0732410:CDS
CCGACAAAGATTACCAACAT+TGG 0.570402 5_1:+44810825 Msa0732410:CDS
AACAATGGACTCACCAGGTG+TGG 0.570501 5_1:-44810423 None:intergenic
GTATTTCAGTTATAGGAAAG+GGG 0.570896 5_1:-44813219 None:intergenic
GTGTGGAGAAGAATTTGTCT+TGG 0.571094 5_1:-44810406 None:intergenic
TTGAAACACTACGTGGAAAG+AGG 0.579791 5_1:+44811033 Msa0732410:CDS
CAGGAATGAGTTGATGGAGA+AGG 0.584159 5_1:-44811109 None:intergenic
ATTAATAATTCCACATACTG+GGG 0.584467 5_1:+44812682 Msa0732410:CDS
TATTTCAGTTATAGGAAAGG+GGG 0.584658 5_1:-44813218 None:intergenic
GTAGTATATTAGGAGAAAGT+GGG 0.594333 5_1:-44810104 None:intergenic
TCTTCCATTCTAAGAAGCAA+AGG 0.595126 5_1:+44810272 Msa0732410:CDS
AAAGGTTCAATCAATAAGCA+TGG 0.595162 5_1:+44811770 Msa0732410:CDS
ACAAAGATTACCAACATTGG+AGG 0.598469 5_1:+44810828 Msa0732410:CDS
GGATACATGTGACGATAACA+AGG 0.600528 5_1:+44813100 Msa0732410:three_prime_UTR
GTGACACTTTCATTTGTACA+CGG 0.608400 5_1:+44813038 Msa0732410:three_prime_UTR
ATGGAAGAAGGTGAGAATGG+TGG 0.608866 5_1:-44810257 None:intergenic
GTTGGAGACTCTCTCAATCG+TGG 0.612428 5_1:+44811065 Msa0732410:CDS
GTGGTTGCCACCTCCAATGT+TGG 0.612456 5_1:-44810838 None:intergenic
AATTGACTTGCCAAGAACAT+GGG 0.614611 5_1:+44810798 Msa0732410:CDS
CACGGAAAAGAGTATAGCTA+CGG 0.614974 5_1:+44813056 Msa0732410:three_prime_UTR
AAGATTACCAACATTGGAGG+TGG 0.615305 5_1:+44810831 Msa0732410:CDS
TATGAAAAGTATGCTTAGAT+GGG 0.615716 5_1:+44812055 Msa0732410:CDS
TCAAGGTGCCATTTGCGGTG+GGG 0.620684 5_1:+44810675 Msa0732410:CDS
TATGGCAGGAGTATAGATTG+GGG 0.621719 5_1:+44812619 Msa0732410:intron
GGATACACGACCACTGTACG+AGG 0.634784 5_1:+44810748 Msa0732410:CDS
AAGCATGGTAGAAATTGGAA+AGG 0.640034 5_1:+44811785 Msa0732410:CDS
CATTGGAGGTGGCAACCACA+CGG 0.642871 5_1:+44810842 Msa0732410:CDS
TCTCCATCAACTCATTCCTG+AGG 0.645543 5_1:+44811112 Msa0732410:CDS
AAAGTATGCTTAGATGGGTG+AGG 0.645663 5_1:+44812060 Msa0732410:CDS
GATTGTCACAATTTCAACTG+AGG 0.649875 5_1:+44812016 Msa0732410:CDS
GCTATGCTGATTGTGTACAT+TGG 0.671303 5_1:+44812881 Msa0732410:CDS
GGCAGGAGTATAGATTGGGG+AGG 0.697427 5_1:+44812622 Msa0732410:intron
TTAGGAAGATCGCAACCGTG+TGG 0.699752 5_1:-44810857 None:intergenic
CGGTGGGGAAGACAGAGGAA+GGG 0.705520 5_1:+44810690 Msa0732410:CDS
GATTGGGGAGGTGAGCCAGG+AGG 0.708567 5_1:+44812634 Msa0732410:CDS
ATTTGCGGTGGGGAAGACAG+AGG 0.711107 5_1:+44810685 Msa0732410:CDS
TATACAATAAGACATCACAA+TGG 0.728350 5_1:-44809956 None:intergenic
TTGATGCTTGAAACACTACG+TGG 0.805901 5_1:+44811026 Msa0732410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATAATAAAAATTGA+TGG - chr5_1:44810008-44810027 None:intergenic 10.0%
!! ACAAAAATAATTATATTTAG+TGG - chr5_1:44811554-44811573 None:intergenic 10.0%
!!! TAATTTTCAAATAATCAAAA+TGG - chr5_1:44811932-44811951 None:intergenic 10.0%
!! TATTATAGATCAATTTAAAT+AGG - chr5_1:44812242-44812261 None:intergenic 10.0%
!! ATTTAAATTGATCTATAATA+TGG + chr5_1:44812242-44812261 Msa0732410:intron 10.0%
!!! AGAAACGAATTTTTTTTTTT+TGG - chr5_1:44810078-44810097 None:intergenic 15.0%
!! TTAAAATCTTATGAATATGT+CGG - chr5_1:44810550-44810569 None:intergenic 15.0%
!!! AATATATTATGCCTTTTTTA+TGG + chr5_1:44810593-44810612 Msa0732410:intron 15.0%
!!! TTTATTTGATGTTTTGTATT+TGG + chr5_1:44810616-44810635 Msa0732410:intron 15.0%
!!! TTATTTGATGTTTTGTATTT+GGG + chr5_1:44810617-44810636 Msa0732410:intron 15.0%
!! TTCACTTTAATGTAATAATT+TGG + chr5_1:44811417-44811436 Msa0732410:intron 15.0%
!! TGAAAATCAACAATAAAAAA+TGG - chr5_1:44811638-44811657 None:intergenic 15.0%
!! AAGTCTAAAAAAATTGAAAA+GGG - chr5_1:44811970-44811989 None:intergenic 15.0%
!!! AATTGTAGAGATTTTTTTTA+AGG - chr5_1:44812348-44812367 None:intergenic 15.0%
!!! AAAAAAAATCTCTACAATTT+TGG + chr5_1:44812349-44812368 Msa0732410:intron 15.0%
!! TAAACAAAACTTTATTCTTT+AGG + chr5_1:44812435-44812454 Msa0732410:intron 15.0%
!! CTAATATTTCAACAAATATA+TGG + chr5_1:44812601-44812620 Msa0732410:intron 15.0%
!! TGAATTATCTATATATTGTT+AGG + chr5_1:44813309-44813328 Msa0732410:three_prime_UTR 15.0%
!!! TTTTAACTATTAGTGTTTTT+TGG - chr5_1:44813351-44813370 None:intergenic 15.0%
!!! ATTTTTTTTTTTTGGTGAAG+TGG - chr5_1:44810070-44810089 None:intergenic 20.0%
!!! TTTTACATCAAAAAGTATAG+AGG + chr5_1:44810483-44810502 Msa0732410:intron 20.0%
!! TAAAATCTTATGAATATGTC+GGG - chr5_1:44810549-44810568 None:intergenic 20.0%
!! CATCAAATAAACCATAAAAA+AGG - chr5_1:44810607-44810626 None:intergenic 20.0%
!!! TATTTGATGTTTTGTATTTG+GGG + chr5_1:44810618-44810637 Msa0732410:intron 20.0%
!! GCATAAATAAATACTTACTT+AGG - chr5_1:44810878-44810897 None:intergenic 20.0%
!! TTCATAACAATAATGAAGAT+CGG - chr5_1:44811205-44811224 None:intergenic 20.0%
!! TTCATTATTGTTATGAATGA+TGG + chr5_1:44811208-44811227 Msa0732410:intron 20.0%
!! AGTTAATTATAAGAGTGAAT+CGG + chr5_1:44811353-44811372 Msa0732410:intron 20.0%
!! CAAATAATCAAAATGGAAAA+GGG - chr5_1:44811925-44811944 None:intergenic 20.0%
!! TCAAATAATCAAAATGGAAA+AGG - chr5_1:44811926-44811945 None:intergenic 20.0%
!! CAAGTCTAAAAAAATTGAAA+AGG - chr5_1:44811971-44811990 None:intergenic 20.0%
!!! CTTTTCAATTTTTTTAGACT+TGG + chr5_1:44811969-44811988 Msa0732410:intron 20.0%
!! ACACATTTGTTCAAATATAA+AGG + chr5_1:44812974-44812993 Msa0732410:three_prime_UTR 20.0%
!! CACATTTGTTCAAATATAAA+GGG + chr5_1:44812975-44812994 Msa0732410:three_prime_UTR 20.0%
!!! ACTACAAAAAATTTGGAATT+TGG - chr5_1:44813244-44813263 None:intergenic 20.0%
!!! TCCAAATTTTTTGTAGTTTT+TGG + chr5_1:44813247-44813266 Msa0732410:three_prime_UTR 20.0%
!! ACCAAAAACTACAAAAAATT+TGG - chr5_1:44813251-44813270 None:intergenic 20.0%
! TATACAATAAGACATCACAA+TGG - chr5_1:44809959-44809978 None:intergenic 25.0%
!! TTGTGATGTCTTATTGTATA+AGG + chr5_1:44809959-44809978 Msa0732410:five_prime_UTR 25.0%
! GAATGTACTAGTAGTATATT+AGG - chr5_1:44810117-44810136 None:intergenic 25.0%
!! TAAATAATAGTGAGTTGAGT+TGG + chr5_1:44810206-44810225 Msa0732410:five_prime_UTR 25.0%
! AAAAAGAATGAAAGCTAGAA+GGG - chr5_1:44810321-44810340 None:intergenic 25.0%
!! CTTTTTGTTACTATTCTCTA+TGG + chr5_1:44810335-44810354 Msa0732410:CDS 25.0%
!!! TGTAGCATTTTTTGAAACAA+TGG - chr5_1:44810441-44810460 None:intergenic 25.0%
! AAAATCTTATGAATATGTCG+GGG - chr5_1:44810548-44810567 None:intergenic 25.0%
!! ATTTGATGTTTTGTATTTGG+GGG + chr5_1:44810619-44810638 Msa0732410:intron 25.0%
! TTGATAAAAGAACGAGATAA+TGG + chr5_1:44811320-44811339 Msa0732410:intron 25.0%
!! TGATTTTCATGTGTTTAATC+AGG + chr5_1:44811649-44811668 Msa0732410:intron 25.0%
!!! TTTTCTTCTAGAATCAAACT+CGG + chr5_1:44811706-44811725 Msa0732410:CDS 25.0%
! TCTGATAGAATTGTAAGAAA+AGG + chr5_1:44811752-44811771 Msa0732410:CDS 25.0%
!!! ATCTTTTGATGATGAAGTAA+AGG + chr5_1:44811992-44812011 Msa0732410:CDS 25.0%
!! TATGAAAAGTATGCTTAGAT+GGG + chr5_1:44812055-44812074 Msa0732410:CDS 25.0%
!!! GTTTTGTTTATAATAGTGAC+CGG - chr5_1:44812425-44812444 None:intergenic 25.0%
!! ATACGCAAAATTTCACATTT+TGG - chr5_1:44812524-44812543 None:intergenic 25.0%
! GAAATATTAGAACTTAGAAG+TGG - chr5_1:44812591-44812610 None:intergenic 25.0%
! TATTTCAACAAATATATGGC+AGG + chr5_1:44812605-44812624 Msa0732410:intron 25.0%
! TGATTAATAATTCCACATAC+TGG + chr5_1:44812680-44812699 Msa0732410:CDS 25.0%
! GATTAATAATTCCACATACT+GGG + chr5_1:44812681-44812700 Msa0732410:CDS 25.0%
! ATTAATAATTCCACATACTG+GGG + chr5_1:44812682-44812701 Msa0732410:CDS 25.0%
!! AAAAGTATAATGCAAGTGAT+TGG + chr5_1:44812718-44812737 Msa0732410:CDS 25.0%
! GTGATGTTAAGAAATGTAAT+TGG - chr5_1:44812769-44812788 None:intergenic 25.0%
! CTAATTGCTCTTTAGTTAAA+GGG - chr5_1:44812849-44812868 None:intergenic 25.0%
!! ACAAATGTGTACAATTTTCA+AGG - chr5_1:44812964-44812983 None:intergenic 25.0%
!! ATCATATATTGTTGTTCTCT+TGG + chr5_1:44812998-44813017 Msa0732410:three_prime_UTR 25.0%
!! TGATTAATCTTTGTTTGCAA+AGG + chr5_1:44813079-44813098 Msa0732410:three_prime_UTR 25.0%
! GAATTTGGTATTTCAGTTAT+AGG - chr5_1:44813229-44813248 None:intergenic 25.0%
TGAACTTATGTCTTTGTTGA+TGG - chr5_1:44810035-44810054 None:intergenic 30.0%
GTAGTATATTAGGAGAAAGT+GGG - chr5_1:44810107-44810126 None:intergenic 30.0%
AGTAGTATATTAGGAGAAAG+TGG - chr5_1:44810108-44810127 None:intergenic 30.0%
! GTACTTTACTCTAAAAGACT+TGG - chr5_1:44810164-44810183 None:intergenic 30.0%
CAAAAAGAATGAAAGCTAGA+AGG - chr5_1:44810322-44810341 None:intergenic 30.0%
! TTTGATGTTTTGTATTTGGG+GGG + chr5_1:44810620-44810639 Msa0732410:intron 30.0%
TGATATATTCAATGGAAGGT+GGG + chr5_1:44810715-44810734 Msa0732410:CDS 30.0%
GAAAACAGTTAGTGAATCAA+AGG - chr5_1:44811156-44811175 None:intergenic 30.0%
AGAAATCATAAGTATTCGTG+TGG + chr5_1:44811267-44811286 Msa0732410:intron 30.0%
GAAATCATAAGTATTCGTGT+GGG + chr5_1:44811268-44811287 Msa0732410:intron 30.0%
AATCAACTAGAGTTAGTTGT+TGG - chr5_1:44811399-44811418 None:intergenic 30.0%
ATGCAACAATTGAGTTCTAT+TGG + chr5_1:44811678-44811697 Msa0732410:CDS 30.0%
TGCAACAATTGAGTTCTATT+GGG + chr5_1:44811679-44811698 Msa0732410:CDS 30.0%
! GAGTTTGATTCTAGAAGAAA+AGG - chr5_1:44811707-44811726 None:intergenic 30.0%
AAAGGTTCAATCAATAAGCA+TGG + chr5_1:44811770-44811789 Msa0732410:CDS 30.0%
TCAATAAGCATGGTAGAAAT+TGG + chr5_1:44811780-44811799 Msa0732410:CDS 30.0%
TGGTATTCAACACTTATCTT+TGG + chr5_1:44811819-44811838 Msa0732410:CDS 30.0%
TATTCAACACTTATCTTTGG+TGG + chr5_1:44811822-44811841 Msa0732410:CDS 30.0%
GCTTGTTTGAATAGAAAAGA+TGG - chr5_1:44811881-44811900 None:intergenic 30.0%
! CTATGAAAAGTATGCTTAGA+TGG + chr5_1:44812054-44812073 Msa0732410:CDS 30.0%
!!! AAGAAGACTCTAGTTTTCTT+TGG - chr5_1:44812098-44812117 None:intergenic 30.0%
! GAAACCAAACCATACAATTT+TGG + chr5_1:44812319-44812338 Msa0732410:intron 30.0%
! TTAAGGTGTCCAAAATTGTA+TGG - chr5_1:44812331-44812350 None:intergenic 30.0%
TGCGTATAATAATGATCAGA+AGG + chr5_1:44812537-44812556 Msa0732410:intron 30.0%
CTTCAATCTATAAGAAGCAA+TGG + chr5_1:44812821-44812840 Msa0732410:CDS 30.0%
GCTAATTGCTCTTTAGTTAA+AGG - chr5_1:44812850-44812869 None:intergenic 30.0%
AAGAAAGAAATGAACGAATG+AGG + chr5_1:44813123-44813142 Msa0732410:three_prime_UTR 30.0%
GTTAGTAGTTGTTCAAAGAA+AGG + chr5_1:44813159-44813178 Msa0732410:three_prime_UTR 30.0%
TATTTCAGTTATAGGAAAGG+GGG - chr5_1:44813221-44813240 None:intergenic 30.0%
GTATTTCAGTTATAGGAAAG+GGG - chr5_1:44813222-44813241 None:intergenic 30.0%
GGTATTTCAGTTATAGGAAA+GGG - chr5_1:44813223-44813242 None:intergenic 30.0%
TGGTATTTCAGTTATAGGAA+AGG - chr5_1:44813224-44813243 None:intergenic 30.0%
!! ATAGTGAGTTGAGTTGGATT+TGG + chr5_1:44810212-44810231 Msa0732410:five_prime_UTR 35.0%
TTGCTTCTTAGAATGGAAGA+AGG - chr5_1:44810272-44810291 None:intergenic 35.0%
! TCTTCCATTCTAAGAAGCAA+AGG + chr5_1:44810272-44810291 Msa0732410:CDS 35.0%
GAAGATTTCATGTGCATCTT+TGG + chr5_1:44810359-44810378 Msa0732410:CDS 35.0%
GGGTGAATAATGAAAAGAGA+GGG + chr5_1:44810640-44810659 Msa0732410:intron 35.0%
! GAAGGGTGTGATATATTCAA+TGG + chr5_1:44810707-44810726 Msa0732410:CDS 35.0%
GGTGTGATATATTCAATGGA+AGG + chr5_1:44810711-44810730 Msa0732410:CDS 35.0%
GTGATATATTCAATGGAAGG+TGG + chr5_1:44810714-44810733 Msa0732410:CDS 35.0%
GTCAATTGAGGTTGTATGTA+CGG - chr5_1:44810785-44810804 None:intergenic 35.0%
!! AATTGACTTGCCAAGAACAT+GGG + chr5_1:44810798-44810817 Msa0732410:CDS 35.0%
!! CAATGTTGGTAATCTTTGTC+GGG - chr5_1:44810827-44810846 None:intergenic 35.0%
!! CCAATGTTGGTAATCTTTGT+CGG - chr5_1:44810828-44810847 None:intergenic 35.0%
ACAAAGATTACCAACATTGG+AGG + chr5_1:44810828-44810847 Msa0732410:CDS 35.0%
ATCAAAGGTTTCCATGGATT+TGG - chr5_1:44811141-44811160 None:intergenic 35.0%
TAGTGAATCAAAGGTTTCCA+TGG - chr5_1:44811147-44811166 None:intergenic 35.0%
! ACTAACTGTTTTCACAGCAA+AGG + chr5_1:44811163-44811182 Msa0732410:CDS 35.0%
TTCACAGCAAAGGTAAATCA+AGG + chr5_1:44811173-44811192 Msa0732410:intron 35.0%
! ACTAGAGTTAGTTGTTGGTT+TGG - chr5_1:44811394-44811413 None:intergenic 35.0%
AAGCATGGTAGAAATTGGAA+AGG + chr5_1:44811785-44811804 Msa0732410:CDS 35.0%
!!! TTGGAAAGGTGTTGACATTT+TGG + chr5_1:44811799-44811818 Msa0732410:CDS 35.0%
GATTGTCACAATTTCAACTG+AGG + chr5_1:44812016-44812035 Msa0732410:CDS 35.0%
TATACTCACTTTCCATGAGA+AGG - chr5_1:44812134-44812153 None:intergenic 35.0%
GTGTCCAAAATTGTATGGTT+TGG - chr5_1:44812326-44812345 None:intergenic 35.0%
! ACATGCTCGTTGTTAATTTG+AGG - chr5_1:44812395-44812414 None:intergenic 35.0%
TGTTTATAATAGTGACCGGA+GGG - chr5_1:44812421-44812440 None:intergenic 35.0%
TTGTTTATAATAGTGACCGG+AGG - chr5_1:44812422-44812441 None:intergenic 35.0%
! TATATGGCAGGAGTATAGAT+TGG + chr5_1:44812617-44812636 Msa0732410:intron 35.0%
! ATATGGCAGGAGTATAGATT+GGG + chr5_1:44812618-44812637 Msa0732410:intron 35.0%
TTGAAGTGTGTGCATCTTTA+CGG - chr5_1:44812807-44812826 None:intergenic 35.0%
! TACCTGGTCTTCAAGATAAT+TGG + chr5_1:44812908-44812927 Msa0732410:CDS 35.0%
TTCCAATTATCTTGAAGACC+AGG - chr5_1:44812913-44812932 None:intergenic 35.0%
! ATTGTTGTTCTCTTGGATCA+TGG + chr5_1:44813005-44813024 Msa0732410:three_prime_UTR 35.0%
GTGACACTTTCATTTGTACA+CGG + chr5_1:44813038-44813057 Msa0732410:three_prime_UTR 35.0%
AGAATGGAAGAAGGTGAGAA+TGG - chr5_1:44810263-44810282 None:intergenic 40.0%
ACGACCTTTGCTTCTTAGAA+TGG - chr5_1:44810279-44810298 None:intergenic 40.0%
TAGAAGGGTGAAAAGGTAAG+GGG - chr5_1:44810306-44810325 None:intergenic 40.0%
CTAGAAGGGTGAAAAGGTAA+GGG - chr5_1:44810307-44810326 None:intergenic 40.0%
TGAAAGCTAGAAGGGTGAAA+AGG - chr5_1:44810313-44810332 None:intergenic 40.0%
GTGTGGAGAAGAATTTGTCT+TGG - chr5_1:44810409-44810428 None:intergenic 40.0%
! TTTGAAACAATGGACTCACC+AGG - chr5_1:44810431-44810450 None:intergenic 40.0%
GCGAGTTTGAACTCTCTTAT+GGG - chr5_1:44810524-44810543 None:intergenic 40.0%
GGGGTGAATAATGAAAAGAG+AGG + chr5_1:44810639-44810658 Msa0732410:intron 40.0%
! TATTCAATGGAAGGTGGGTT+TGG + chr5_1:44810720-44810739 Msa0732410:CDS 40.0%
! ATTCAATGGAAGGTGGGTTT+GGG + chr5_1:44810721-44810740 Msa0732410:CDS 40.0%
TGTTCTTGGCAAGTCAATTG+AGG - chr5_1:44810797-44810816 None:intergenic 40.0%
!! CAATTGACTTGCCAAGAACA+TGG + chr5_1:44810797-44810816 Msa0732410:CDS 40.0%
CCGACAAAGATTACCAACAT+TGG + chr5_1:44810825-44810844 Msa0732410:CDS 40.0%
AAGATTACCAACATTGGAGG+TGG + chr5_1:44810831-44810850 Msa0732410:CDS 40.0%
TTGATGCTTGAAACACTACG+TGG + chr5_1:44811026-44811045 Msa0732410:CDS 40.0%
TTGAAACACTACGTGGAAAG+AGG + chr5_1:44811033-44811052 Msa0732410:CDS 40.0%
GGAAAGAGGATGATGTTTGT+TGG + chr5_1:44811047-44811066 Msa0732410:CDS 40.0%
TAGAGATCATAGTGATCAGC+AGG + chr5_1:44811242-44811261 Msa0732410:intron 40.0%
! ACTTATCTTTGGTGGATGAC+TGG + chr5_1:44811830-44811849 Msa0732410:CDS 40.0%
AACTGAGGATGCTTATCGTA+TGG + chr5_1:44812031-44812050 Msa0732410:CDS 40.0%
AAAGTATGCTTAGATGGGTG+AGG + chr5_1:44812060-44812079 Msa0732410:CDS 40.0%
! TATGGCAGGAGTATAGATTG+GGG + chr5_1:44812619-44812638 Msa0732410:intron 40.0%
TCATTGTAACAACTTCCTCC+TGG - chr5_1:44812652-44812671 None:intergenic 40.0%
CACAATCAGCATAGCTTACA+GGG - chr5_1:44812876-44812895 None:intergenic 40.0%
ACACAATCAGCATAGCTTAC+AGG - chr5_1:44812877-44812896 None:intergenic 40.0%
! GCTATGCTGATTGTGTACAT+TGG + chr5_1:44812881-44812900 Msa0732410:CDS 40.0%
! TGTGTACATTGGTGTTTACC+TGG + chr5_1:44812892-44812911 Msa0732410:CDS 40.0%
! CACGGAAAAGAGTATAGCTA+CGG + chr5_1:44813056-44813075 Msa0732410:three_prime_UTR 40.0%
GGATACATGTGACGATAACA+AGG + chr5_1:44813100-44813119 Msa0732410:three_prime_UTR 40.0%
ATGGAAGAAGGTGAGAATGG+TGG - chr5_1:44810260-44810279 None:intergenic 45.0%
GCTAGAAGGGTGAAAAGGTA+AGG - chr5_1:44810308-44810327 None:intergenic 45.0%
GACAAATTCTTCTCCACACC+TGG + chr5_1:44810410-44810429 Msa0732410:CDS 45.0%
CGCGAGTTTGAACTCTCTTA+TGG - chr5_1:44810525-44810544 None:intergenic 45.0%
!! CAGGAATGAGTTGATGGAGA+AGG - chr5_1:44811112-44811131 None:intergenic 45.0%
TCTCCATCAACTCATTCCTG+AGG + chr5_1:44811112-44811131 Msa0732410:CDS 45.0%
! CATCCTCAGGAATGAGTTGA+TGG - chr5_1:44811118-44811137 None:intergenic 45.0%
CAAGCAACAACGTTAACACG+CGG - chr5_1:44811460-44811479 None:intergenic 45.0%
CGGACAATGCTGTTGTTCAT+AGG + chr5_1:44811726-44811745 Msa0732410:CDS 45.0%
ACTAGCATGTCACCTTCTCA+TGG + chr5_1:44812119-44812138 Msa0732410:CDS 45.0%
ACAATCAGAACCCCAGTATG+TGG - chr5_1:44812695-44812714 None:intergenic 45.0%
TCTCTTGGATCATGGACTCT+TGG + chr5_1:44813013-44813032 Msa0732410:three_prime_UTR 45.0%
CTCTTGGATCATGGACTCTT+GGG + chr5_1:44813014-44813033 Msa0732410:three_prime_UTR 45.0%
! AACAATGGACTCACCAGGTG+TGG - chr5_1:44810426-44810445 None:intergenic 50.0%
GAGGGTGAAGAAGCAGATCA+AGG + chr5_1:44810658-44810677 Msa0732410:CDS 50.0%
!! GCAGATCAAGGTGCCATTTG+CGG + chr5_1:44810670-44810689 Msa0732410:CDS 50.0%
!! ATCAAGGTGCCATTTGCGGT+GGG + chr5_1:44810674-44810693 Msa0732410:CDS 50.0%
ACGACCACTGTACGAGGAAT+CGG + chr5_1:44810754-44810773 Msa0732410:CDS 50.0%
TTAGGAAGATCGCAACCGTG+TGG - chr5_1:44810860-44810879 None:intergenic 50.0%
GTTGGAGACTCTCTCAATCG+TGG + chr5_1:44811065-44811084 Msa0732410:CDS 50.0%
TCCTGAGGATGCCAAATCCA+TGG + chr5_1:44811127-44811146 Msa0732410:CDS 50.0%
TCCATGGATTTGGCATCCTC+AGG - chr5_1:44811131-44811150 None:intergenic 50.0%
GTTAACACGCGGAGGATACT+TGG - chr5_1:44811449-44811468 None:intergenic 50.0%
! TCTTTGGTGGATGACTGGCT+TGG + chr5_1:44811835-44811854 Msa0732410:CDS 50.0%
!! GATCAAGGTGCCATTTGCGG+TGG + chr5_1:44810673-44810692 Msa0732410:CDS 55.0%
!! TCAAGGTGCCATTTGCGGTG+GGG + chr5_1:44810675-44810694 Msa0732410:CDS 55.0%
TCTGTCTTCCCCACCGCAAA+TGG - chr5_1:44810686-44810705 None:intergenic 55.0%
ATTTGCGGTGGGGAAGACAG+AGG + chr5_1:44810685-44810704 Msa0732410:CDS 55.0%
GGATACACGACCACTGTACG+AGG + chr5_1:44810748-44810767 Msa0732410:CDS 55.0%
CACTCCGATTCCTCGTACAG+TGG - chr5_1:44810761-44810780 None:intergenic 55.0%
TGTCGGGTCTCCCATGTTCT+TGG - chr5_1:44810811-44810830 None:intergenic 55.0%
GTGGTTGCCACCTCCAATGT+TGG - chr5_1:44810841-44810860 None:intergenic 55.0%
CATTGGAGGTGGCAACCACA+CGG + chr5_1:44810842-44810861 Msa0732410:CDS 55.0%
GCAACAACGTTAACACGCGG+AGG - chr5_1:44811457-44811476 None:intergenic 55.0%
CAACGAGCATGTACACCCTC+CGG + chr5_1:44812403-44812422 Msa0732410:intron 55.0%
! GGCAGGAGTATAGATTGGGG+AGG + chr5_1:44812622-44812641 Msa0732410:intron 55.0%
ATAGATTGGGGAGGTGAGCC+AGG + chr5_1:44812631-44812650 Msa0732410:CDS 55.0%
CGGTGGGGAAGACAGAGGAA+GGG + chr5_1:44810690-44810709 Msa0732410:CDS 60.0%
! TGGAAGGTGGGTTTGGGACG+AGG + chr5_1:44810727-44810746 Msa0732410:CDS 60.0%
GCGGTGGGGAAGACAGAGGA+AGG + chr5_1:44810689-44810708 Msa0732410:CDS 65.0%
GATTGGGGAGGTGAGCCAGG+AGG + chr5_1:44812634-44812653 Msa0732410:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 44809949 44813375 44809949 ID=Msa0732410;Name=Msa0732410
chr5_1 mRNA 44809949 44813375 44809949 ID=Msa0732410-mRNA-1;Parent=Msa0732410;Name=Msa0732410-mRNA-1;_AED=0.19;_eAED=0.19;_QI=293|1|1|1|1|1|6|409|427
chr5_1 exon 44809949 44810431 44809949 ID=Msa0732410-mRNA-1:exon:12700;Parent=Msa0732410-mRNA-1
chr5_1 exon 44810657 44810879 44810657 ID=Msa0732410-mRNA-1:exon:12701;Parent=Msa0732410-mRNA-1
chr5_1 exon 44811013 44811184 44811013 ID=Msa0732410-mRNA-1:exon:12702;Parent=Msa0732410-mRNA-1
chr5_1 exon 44811671 44811870 44811671 ID=Msa0732410-mRNA-1:exon:12703;Parent=Msa0732410-mRNA-1
chr5_1 exon 44811986 44812144 44811986 ID=Msa0732410-mRNA-1:exon:12704;Parent=Msa0732410-mRNA-1
chr5_1 exon 44812627 44813375 44812627 ID=Msa0732410-mRNA-1:exon:12705;Parent=Msa0732410-mRNA-1
chr5_1 five_prime_UTR 44809949 44810241 44809949 ID=Msa0732410-mRNA-1:five_prime_utr;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44810242 44810431 44810242 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44810657 44810879 44810657 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44811013 44811184 44811013 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44811671 44811870 44811671 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44811986 44812144 44811986 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 CDS 44812627 44812966 44812627 ID=Msa0732410-mRNA-1:cds;Parent=Msa0732410-mRNA-1
chr5_1 three_prime_UTR 44812967 44813375 44812967 ID=Msa0732410-mRNA-1:three_prime_utr;Parent=Msa0732410-mRNA-1
Gene Sequence

>Msa0732410

ATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCACTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGAGTATAGATTGGGGAGGTGAGCCAGGAGGAAGTTGTTACAATGAAACAACTTTGATTAATAATTCCACATACTGGGGTTCTGATTGTAGAAAAAGTATAATGCAAGTGATTGGTGAAGTGTTAAGCAAAACAAAAGTTCCAATTACATTTCTTAACATCACACAACTCTCAAGTTACCGTAAAGATGCACACACTTCAATCTATAAGAAGCAATGGAGCCCTTTAACTAAAGAGCAATTAGCTAACCCTGTAAGCTATGCTGATTGTGTACATTGGTGTTTACCTGGTCTTCAAGATAATTGGAATGAACTTTTGTTTGCTAAGTTGTTTTATCCTTGA
Protein sequence

>Msa0732410

MQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGKSIDWGGEPGGSCYNETTLINNSTYWGSDCRKSIMQVIGEVLSKTKVPITFLNITQLSSYRKDAHTSIYKKQWSPLTKEQLANPVSYADCVHWCLPGLQDNWNELLFAKLFYP*