AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0818710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0818710 MtrunA17_Chr5g0437601 99.297 427 3 0 1 427 1 427 0.0 890
Msa0818710 MtrunA17_Chr3g0114101 74.596 433 101 5 4 427 1 433 0.0 660
Msa0818710 MtrunA17_Chr4g0005491 51.003 349 165 3 83 426 62 409 2.44e-132 387
Msa0818710 MtrunA17_Chr7g0243301 48.677 378 183 5 53 425 39 410 4.74e-132 386
Msa0818710 MtrunA17_Chr4g0005481 52.187 343 161 2 79 421 104 443 3.05e-129 380
Msa0818710 MtrunA17_Chr4g0006991 48.986 345 174 2 81 424 84 427 9.86e-129 378
Msa0818710 MtrunA17_Chr8g0360521 49.025 359 175 4 71 425 56 410 5.42e-128 376
Msa0818710 MtrunA17_Chr3g0113671 51.289 349 164 4 80 424 103 449 1.25e-126 374
Msa0818710 MtrunA17_Chr5g0437291 48.571 350 175 3 81 425 163 512 9.06e-119 356
Msa0818710 MtrunA17_Chr7g0242701 41.647 425 238 4 10 424 3 427 1.09e-118 352
Msa0818710 MtrunA17_Chr5g0430371 36.675 379 207 9 64 426 40 401 1.24e-76 244
Msa0818710 MtrunA17_Chr3g0105301 36.944 360 201 8 76 426 36 378 1.57e-76 243
Msa0818710 MtrunA17_Chr5g0413271 36.932 352 206 8 77 425 139 477 2.08e-75 243
Msa0818710 MtrunA17_Chr4g0041011 34.097 393 226 9 32 424 4 363 3.49e-75 239
Msa0818710 MtrunA17_Chr1g0210771 38.329 347 186 10 83 424 59 382 1.11e-74 238
Msa0818710 MtrunA17_Chr2g0325481 37.026 343 191 7 83 425 50 367 2.62e-74 236
Msa0818710 MtrunA17_Chr1g0175671 36.723 354 200 8 83 425 170 510 1.90e-73 239
Msa0818710 MtrunA17_Chr3g0097891 35.127 353 203 8 75 425 23 351 1.39e-72 231
Msa0818710 MtrunA17_Chr7g0258851 37.255 357 194 9 83 425 248 588 1.79e-72 238
Msa0818710 MtrunA17_Chr1g0171171 33.668 398 234 10 27 424 8 375 6.79e-72 231
Msa0818710 MtrunA17_Chr7g0259711 33.034 445 258 15 2 425 8 433 1.59e-71 232
Msa0818710 MtrunA17_Chr4g0071391 37.143 350 200 8 83 424 109 446 2.05e-71 232
Msa0818710 MtrunA17_Chr2g0287901 37.183 355 196 11 81 422 95 435 5.72e-70 228
Msa0818710 MtrunA17_Chr2g0314801 36.286 350 192 8 77 421 27 350 9.66e-69 222
Msa0818710 MtrunA17_Chr2g0283581 34.925 398 211 11 27 424 9 358 2.57e-68 221
Msa0818710 MtrunA17_Chr7g0269991 36.648 352 192 9 83 424 137 467 5.71e-68 223
Msa0818710 MtrunA17_Chr3g0085081 35.817 349 190 10 81 424 29 348 1.29e-67 219
Msa0818710 MtrunA17_Chr5g0413851 37.644 348 189 10 79 424 38 359 1.54e-67 219
Msa0818710 MtrunA17_Chr4g0045941 33.903 351 208 8 75 425 25 351 1.55e-66 216
Msa0818710 MtrunA17_Chr3g0085071 35.817 349 190 10 81 424 44 363 2.31e-66 216
Msa0818710 MtrunA17_Chr1g0213281 33.943 383 231 10 56 426 145 517 2.39e-66 220
Msa0818710 MtrunA17_Chr4g0068111 33.711 353 212 7 83 425 114 454 7.10e-66 218
Msa0818710 MtrunA17_Chr3g0091031 35.244 349 192 10 81 424 29 348 2.71e-65 213
Msa0818710 MtrunA17_Chr8g0368461 33.333 372 213 10 76 425 171 529 3.28e-63 213
Msa0818710 MtrunA17_Chr4g0045951 33.333 348 208 8 78 425 40 363 1.50e-61 203
Msa0818710 MtrunA17_Chr2g0314791 35.393 356 185 13 80 425 26 346 2.01e-60 200
Msa0818710 MtrunA17_Chr8g0384151 28.440 436 260 13 5 425 19 417 6.83e-55 188
Msa0818710 MtrunA17_Chr3g0126021 29.609 358 219 8 79 425 125 460 2.06e-52 182
Msa0818710 MtrunA17_Chr6g0456681 30.833 360 220 10 81 424 62 408 3.83e-43 156
Msa0818710 MtrunA17_Chr1g0209921 34.043 235 143 6 81 313 42 266 3.91e-42 149
Msa0818710 MtrunA17_Chr1g0188191 27.889 398 254 16 43 421 195 578 4.66e-40 150
Msa0818710 MtrunA17_Chr1g0178081 29.261 352 180 9 81 425 53 342 5.44e-40 146
Msa0818710 MtrunA17_Chr6g0456691 27.419 434 262 16 16 424 14 419 3.69e-39 145
Msa0818710 MtrunA17_Chr8g0347321 28.571 357 228 12 83 424 95 439 6.88e-39 145
Msa0818710 MtrunA17_Chr8g0384421 30.028 363 214 13 81 424 131 472 2.46e-37 141
Msa0818710 MtrunA17_Chr6g0476041 28.011 357 231 14 81 421 165 511 3.29e-37 142
Msa0818710 MtrunA17_Chr8g0391481 29.428 367 219 15 80 424 24 372 2.38e-36 137
Msa0818710 MtrunA17_Chr6g0451321 28.032 371 229 14 73 424 106 457 5.76e-36 137
Msa0818710 MtrunA17_Chr5g0414061 27.887 355 227 12 83 421 94 435 4.22e-35 135
Msa0818710 MtrunA17_Chr5g0414091 28.453 362 222 12 80 420 82 427 8.64e-35 134
Msa0818710 MtrunA17_Chr2g0283851 27.559 381 230 15 65 420 37 396 8.87e-35 133
Msa0818710 MtrunA17_Chr5g0414051 25.886 367 226 13 80 421 72 417 4.38e-33 129
Msa0818710 MtrunA17_Chr3g0140371 28.333 360 223 13 80 421 15 357 9.76e-33 127
Msa0818710 MtrunA17_Chr7g0226991 27.348 362 227 14 80 421 54 399 1.43e-32 127
Msa0818710 MtrunA17_Chr8g0387921 26.479 355 231 12 83 420 74 415 2.71e-32 127
Msa0818710 MtrunA17_Chr5g0414041 28.571 357 224 12 83 421 87 430 1.44e-31 125
Msa0818710 MtrunA17_Chr4g0049421 26.997 363 218 16 83 420 48 388 1.69e-29 118
Msa0818710 MtrunA17_Chr6g0455901 26.503 366 231 12 80 424 68 416 9.03e-29 117
Msa0818710 MtrunA17_Chr4g0070341 27.704 379 202 16 81 424 130 471 2.22e-27 113
Msa0818710 MtrunA17_Chr7g0221271 40.000 130 77 1 81 210 77 205 4.19e-26 104
Msa0818710 MtrunA17_Chr7g0221291 45.361 97 52 1 79 175 75 170 1.71e-24 99.4
Msa0818710 MtrunA17_Chr2g0287931 36.134 119 66 4 143 252 1 118 1.77e-15 72.8
Msa0818710 MtrunA17_Chr3g0085101 38.318 107 62 3 280 385 5 108 6.20e-13 65.5
Msa0818710 MtrunA17_Chr7g0221281 25.311 241 155 10 197 424 8 236 7.06e-12 65.5
Msa0818710 MtrunA17_Chr2g0287911 43.750 64 28 2 76 139 54 109 1.23e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0818710 AT2G40320.1 78.537 410 86 1 17 426 17 424 0.0 689
Msa0818710 AT3G11030.1 62.723 448 137 4 8 427 6 451 0.0 588
Msa0818710 AT5G01620.2 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa0818710 AT5G01620.1 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa0818710 AT5G01620.3 50.000 350 171 2 73 421 108 454 1.00e-128 379
Msa0818710 AT3G55990.1 53.009 349 158 3 81 425 138 484 4.94e-128 379
Msa0818710 AT1G73140.1 44.444 378 193 3 49 425 45 406 7.02e-127 373
Msa0818710 AT5G01360.2 48.696 345 176 1 81 424 34 378 1.68e-126 371
Msa0818710 AT2G40150.1 51.233 365 163 4 73 424 58 420 5.92e-126 371
Msa0818710 AT5G01360.1 48.696 345 176 1 81 424 90 434 6.81e-126 371
Msa0818710 AT2G38320.1 44.865 370 198 3 61 425 37 405 8.04e-117 347
Msa0818710 AT2G40160.1 40.821 414 229 7 23 426 18 425 1.70e-107 324
Msa0818710 AT2G40160.2 41.176 408 224 7 23 420 18 419 4.32e-107 323
Msa0818710 AT2G38320.2 47.188 320 163 3 111 425 4 322 1.18e-105 316
Msa0818710 AT3G12060.1 38.259 379 204 9 61 425 174 536 3.80e-82 263
Msa0818710 AT5G06700.1 38.547 358 192 10 81 425 253 595 1.11e-78 255
Msa0818710 AT3G62390.1 37.166 374 203 11 68 426 117 473 1.97e-75 243
Msa0818710 AT1G78710.1 35.429 350 199 8 78 425 33 357 3.19e-75 239
Msa0818710 AT5G20590.1 36.639 363 209 8 72 425 130 480 3.68e-74 240
Msa0818710 AT2G40160.3 39.799 299 166 5 23 313 18 310 3.78e-74 234
Msa0818710 AT5G19160.1 34.651 430 237 14 20 422 30 442 5.21e-74 239
Msa0818710 AT3G14850.2 37.249 349 188 7 78 421 29 351 9.90e-73 233
Msa0818710 AT2G30900.1 35.135 370 204 12 68 426 23 367 4.13e-72 231
Msa0818710 AT3G06080.2 32.118 439 247 12 15 422 25 443 1.82e-70 230
Msa0818710 AT5G58600.1 35.411 353 203 7 83 426 66 402 2.22e-70 228
Msa0818710 AT5G49340.1 35.230 369 219 9 68 425 83 442 2.39e-70 229
Msa0818710 AT1G29050.1 34.582 347 203 6 78 424 56 378 4.46e-70 226
Msa0818710 AT2G30010.1 35.309 388 214 10 54 426 33 398 5.49e-70 227
Msa0818710 AT2G42570.1 34.140 372 217 9 54 425 22 365 1.17e-69 225
Msa0818710 AT2G30010.2 35.309 388 214 10 54 426 76 441 1.61e-69 227
Msa0818710 AT2G34070.1 34.473 351 199 8 78 424 60 383 2.36e-69 224
Msa0818710 AT2G31110.2 36.047 344 192 9 83 424 45 362 7.11e-69 223
Msa0818710 AT1G60790.1 34.551 356 212 9 81 425 188 533 2.02e-68 226
Msa0818710 AT3G54260.1 36.578 339 195 8 83 420 51 370 8.23e-67 218
Msa0818710 AT5G06230.2 34.426 366 196 11 83 425 21 365 3.10e-61 203
Msa0818710 AT5G06230.1 34.426 366 196 11 83 425 62 406 1.14e-60 203
Msa0818710 AT1G48880.1 30.095 422 232 14 23 425 63 440 4.01e-57 194
Msa0818710 AT1G78710.2 30.747 348 165 8 78 425 26 297 2.00e-55 186
Msa0818710 AT5G19160.2 34.505 313 186 8 20 317 30 338 1.45e-54 185
Msa0818710 AT3G11570.1 33.828 337 198 7 100 425 98 420 2.34e-54 186
Msa0818710 AT3G62390.2 38.113 265 143 8 68 317 117 375 9.30e-52 179
Msa0818710 AT3G06080.1 35.743 249 146 5 76 313 93 338 6.06e-50 172
Msa0818710 AT5G20590.2 37.450 251 144 5 72 313 130 376 1.88e-49 172
Msa0818710 AT2G30900.2 35.271 258 145 8 68 315 23 268 8.85e-47 162
Msa0818710 AT2G31110.3 35.088 228 135 5 83 308 45 261 1.98e-44 156
Msa0818710 AT1G70230.1 29.609 358 221 11 78 424 74 411 2.07e-43 157
Msa0818710 AT5G58600.2 33.473 239 140 5 83 315 66 291 1.16e-42 152
Msa0818710 AT2G34070.2 32.364 275 156 7 154 424 1 249 2.64e-42 150
Msa0818710 AT3G14850.1 34.066 273 150 6 154 421 1 248 4.96e-42 149
Msa0818710 AT3G28150.1 28.000 425 245 16 18 422 25 408 1.89e-41 152
Msa0818710 AT5G06230.3 34.524 252 134 7 83 313 62 303 9.25e-41 147
Msa0818710 AT5G15900.1 29.082 392 247 12 50 424 32 409 4.75e-40 148
Msa0818710 AT5G15900.2 29.082 392 247 12 50 424 51 428 5.81e-40 148
Msa0818710 AT5G15890.1 28.652 356 220 10 80 421 184 519 3.14e-38 145
Msa0818710 AT5G64020.1 29.301 372 213 16 83 424 57 408 1.11e-37 141
Msa0818710 AT5G64470.3 29.915 351 218 13 83 420 55 390 3.09e-37 140
Msa0818710 AT3G02440.1 26.966 356 232 11 83 424 127 468 7.38e-37 140
Msa0818710 AT3G02440.2 26.966 356 232 11 83 424 135 476 1.03e-36 140
Msa0818710 AT5G64470.2 29.494 356 219 12 83 420 55 396 2.05e-36 138
Msa0818710 AT1G01430.1 29.661 354 224 12 83 420 99 443 1.81e-35 136
Msa0818710 AT4G25360.1 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa0818710 AT4G25360.2 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa0818710 AT4G23790.1 25.059 427 276 17 21 424 22 427 8.86e-35 134
Msa0818710 AT5G51640.1 27.174 368 225 15 80 421 135 485 4.38e-34 133
Msa0818710 AT2G37720.1 28.571 385 218 17 66 424 129 482 5.56e-34 132
Msa0818710 AT4G01080.1 29.429 350 221 14 83 420 91 426 1.05e-33 131
Msa0818710 AT2G14530.1 26.519 362 229 10 83 424 62 406 1.17e-33 130
Msa0818710 AT2G31110.1 33.621 232 127 8 195 424 8 214 6.24e-31 118
Msa0818710 AT5G20680.6 26.178 382 208 16 79 424 114 457 1.06e-29 120
Msa0818710 AT5G20680.2 26.178 382 208 16 79 424 190 533 1.77e-29 120
Msa0818710 AT5G20680.4 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0818710 AT5G20680.1 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0818710 AT5G20680.3 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0818710 AT5G20680.5 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa0818710 AT4G11090.1 23.040 421 288 15 23 421 16 422 7.15e-27 112
Msa0818710 AT5G64470.1 27.985 268 178 9 83 340 55 317 3.72e-23 99.8
Msa0818710 AT5G64470.4 27.715 267 178 9 83 339 55 316 1.27e-22 98.2

Find 135 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATGGAGTTCCCTCTTT+TGG 0.157557 5_3:+54725678 None:intergenic
TACCTGGTCTTCAAGATAAT+TGG 0.191574 5_3:-54722777 Msa0818710:CDS
TTGGAAAGGTGTTGACATTT+TGG 0.194283 5_3:-54723885 Msa0818710:CDS
GCTAATTGCTCTTTAGTTAA+AGG 0.201296 5_3:+54722838 None:intergenic
ACTCCATTGTGATGTCTTAT+TGG 0.212538 5_3:-54725666 Msa0818710:five_prime_UTR
ACACATTTGTTCAAATATAA+AGG 0.225938 5_3:-54722711 Msa0818710:three_prime_UTR
TGATTAATAATTCCACATAC+TGG 0.251655 5_3:-54723005 Msa0818710:CDS
TGTCGGGTCTCCCATGTTCT+TGG 0.253030 5_3:+54724875 None:intergenic
ATGCAACAATTGAGTTCTAT+TGG 0.254941 5_3:-54724006 Msa0818710:CDS
GAATTTGGTATTTCAGTTAT+AGG 0.257936 5_3:+54722463 None:intergenic
TCAATAAGCATGGTAGAAAT+TGG 0.262180 5_3:-54723904 Msa0818710:CDS
ATTGAATGTACTAGTATATT+AGG 0.272300 5_3:+54725566 None:intergenic
TTGAAGTGTGTGCATCTTTA+CGG 0.287806 5_3:+54722881 None:intergenic
TGAATTATCTATATATTGTT+AGG 0.290624 5_3:-54722380 Msa0818710:three_prime_UTR
ATCAAAGGTTTCCATGGATT+TGG 0.299044 5_3:+54724546 None:intergenic
ATCATATATTGTTGTTCTCT+TGG 0.308084 5_3:-54722687 Msa0818710:three_prime_UTR
CTAATTGCTCTTTAGTTAAA+GGG 0.317364 5_3:+54722839 None:intergenic
GAAGATTTCATGTGCATCTT+TGG 0.320970 5_3:-54725324 Msa0818710:CDS
TATTCAATGGAAGGTGGGTT+TGG 0.326490 5_3:-54724963 Msa0818710:CDS
TGAACTTATGTCTTTGTTGA+TGG 0.335672 5_3:+54725614 None:intergenic
CTCCATTGTGATGTCTTATT+GGG 0.337032 5_3:-54725665 Msa0818710:five_prime_UTR
GTGATGTTAAGAAATGTAAT+TGG 0.337450 5_3:+54722919 None:intergenic
TGGTATTCAACACTTATCTT+TGG 0.343420 5_3:-54723865 Msa0818710:CDS
TCTCTTGGATCATGGACTCT+TGG 0.348285 5_3:-54722672 Msa0818710:three_prime_UTR
ACACAATCAGCATAGCTTAC+AGG 0.357812 5_3:+54722811 None:intergenic
CACATTTGTTCAAATATAAA+GGG 0.368100 5_3:-54722710 Msa0818710:three_prime_UTR
ATTCAATGGAAGGTGGGTTT+GGG 0.383497 5_3:-54724962 Msa0818710:CDS
CGGACAATGCTGTTGTTCAT+AGG 0.387335 5_3:-54723958 Msa0818710:CDS
CTCTTGGATCATGGACTCTT+GGG 0.388166 5_3:-54722671 Msa0818710:three_prime_UTR
ATTGTTGTTCTCTTGGATCA+TGG 0.391390 5_3:-54722680 Msa0818710:three_prime_UTR
ACGACCTTTGCTTCTTAGAA+TGG 0.393423 5_3:+54725407 None:intergenic
CCAATGTTGGTAATCTTTGT+CGG 0.403476 5_3:+54724858 None:intergenic
CTATGAAAAGTATGCTTAGA+TGG 0.410597 5_3:-54723630 Msa0818710:CDS
TCTGTCTTCCCCACCGCAAA+TGG 0.411531 5_3:+54725000 None:intergenic
TGCAACAATTGAGTTCTATT+GGG 0.419007 5_3:-54724005 Msa0818710:CDS
GGTATTTCAGTTATAGGAAA+GGG 0.419698 5_3:+54722469 None:intergenic
TATTCAACACTTATCTTTGG+TGG 0.424745 5_3:-54723862 Msa0818710:CDS
TCCATGGATTTGGCATCCTC+AGG 0.427436 5_3:+54724556 None:intergenic
AAATTAGTTATACGTATATG+AGG 0.427945 5_3:-54725715 Msa0818710:five_prime_UTR
ACTAGTATATTAGGAGAAAG+TGG 0.429923 5_3:+54725575 None:intergenic
ACTTATCTTTGGTGGATGAC+TGG 0.435500 5_3:-54723854 Msa0818710:CDS
ATATGGCAGGAGTATAGATT+GGG 0.440954 5_3:-54723067 Msa0818710:intron
TATATGGCAGGAGTATAGAT+TGG 0.441176 5_3:-54723068 Msa0818710:intron
TAAATAATAGTGAGTTGAGT+TGG 0.441530 5_3:-54725477 Msa0818710:five_prime_UTR
ATAGATTGGGGAGGTGAGCC+AGG 0.443479 5_3:-54723054 Msa0818710:CDS
GTTAGTAGTTGTTCAAAGAA+AGG 0.448939 5_3:-54722530 Msa0818710:three_prime_UTR
ATAGTGAGTTGAGTTGGATT+TGG 0.450733 5_3:-54725471 Msa0818710:five_prime_UTR
CATCCTCAGGAATGAGTTGA+TGG 0.450737 5_3:+54724569 None:intergenic
CAATGTTGGTAATCTTTGTC+GGG 0.457780 5_3:+54724859 None:intergenic
CACTCCGATTCCTCGTACAA+TGG 0.458569 5_3:+54724925 None:intergenic
GCTAGAAGGGTGAAAAGGTA+AGG 0.463485 5_3:+54725378 None:intergenic
TGAAAGCTAGAAGGGTGAAA+AGG 0.463624 5_3:+54725373 None:intergenic
ACGACCATTGTACGAGGAAT+CGG 0.464432 5_3:-54724929 Msa0818710:CDS
GATTAATAATTCCACATACT+GGG 0.472858 5_3:-54723004 Msa0818710:CDS
TGTGTACATTGGTGTTTACC+TGG 0.477328 5_3:-54722793 Msa0818710:CDS
TGGAAGGTGGGTTTGGGACG+AGG 0.477341 5_3:-54724956 Msa0818710:CDS
CACAATCAGCATAGCTTACA+GGG 0.477761 5_3:+54722812 None:intergenic
AAAAGTATAATGCAAGTGAT+TGG 0.480921 5_3:-54722967 Msa0818710:CDS
TCTGATAGAATTGTAAGAAA+AGG 0.484458 5_3:-54723932 Msa0818710:CDS
TGATTAATCTTTGTTTGCAA+AGG 0.491547 5_3:-54722606 Msa0818710:three_prime_UTR
TAGTGAATCAAAGGTTTCCA+TGG 0.492850 5_3:+54724540 None:intergenic
GGAAAGAGGATGATGTTTGT+TGG 0.493089 5_3:-54724637 Msa0818710:CDS
GCAGATCAAGGTGCCATTTG+CGG 0.494304 5_3:-54725013 Msa0818710:CDS
GAAGGGTGTGATATATTCAA+TGG 0.494550 5_3:-54724976 Msa0818710:CDS
GTGATATATTCAATGGAAGG+TGG 0.495098 5_3:-54724969 Msa0818710:CDS
TCTTTGGTGGATGACTGGCT+TGG 0.496770 5_3:-54723849 Msa0818710:CDS
TTGTGATGTCTTATTGGGTA+AGG 0.497193 5_3:-54725660 Msa0818710:five_prime_UTR
TCATTGTAACAACTTCCTCC+TGG 0.501058 5_3:+54723036 None:intergenic
GAGTTTGATTCTAGAAGAAA+AGG 0.501756 5_3:+54723980 None:intergenic
TGTTCTTGGCAAGTCAATTG+AGG 0.502642 5_3:+54724889 None:intergenic
AACTGAGGATGCTTATCGTA+TGG 0.502726 5_3:-54723653 Msa0818710:CDS
CTAGTATATTAGGAGAAAGT+GGG 0.504465 5_3:+54725576 None:intergenic
CAATTGACTTGCCAAGAACA+TGG 0.509598 5_3:-54724886 Msa0818710:CDS
GTCAATTGAGGTTGTATGTA+CGG 0.512274 5_3:+54724901 None:intergenic
GACAAATTCTTCTCCACACC+TGG 0.515054 5_3:-54725273 Msa0818710:intron
ACTAGCATGTCACCTTCTCA+TGG 0.524451 5_3:-54723565 Msa0818710:CDS
ACCACTTAAGTGCCCAAAAG+AGG 0.525177 5_3:-54725691 Msa0818710:five_prime_UTR
TTCCAATTATCTTGAAGACC+AGG 0.528264 5_3:+54722775 None:intergenic
GAAAACAGTTAGTGAATCAA+AGG 0.528871 5_3:+54724531 None:intergenic
TGATATATTCAATGGAAGGT+GGG 0.529302 5_3:-54724968 Msa0818710:CDS
CTAGAAGGGTGAAAAGGTAA+GGG 0.530481 5_3:+54725379 None:intergenic
AGAATGGAAGAAGGTGAGAA+TGG 0.531203 5_3:+54725423 None:intergenic
GCGGTGGGGAAGACAGAGGA+AGG 0.532473 5_3:-54724994 Msa0818710:CDS
ACAATCAGAACCCCAGTATG+TGG 0.541987 5_3:+54722993 None:intergenic
TTGCTTCTTAGAATGGAAGA+AGG 0.542128 5_3:+54725414 None:intergenic
CTTCAATCTATAAGAAGCAA+TGG 0.542329 5_3:-54722864 Msa0818710:CDS
TGGTATTTCAGTTATAGGAA+AGG 0.542790 5_3:+54722468 None:intergenic
TCCTGAGGATGCCAAATCCA+TGG 0.545590 5_3:-54724557 Msa0818710:CDS
GTACTTTACTCTAAAAGACT+TGG 0.546625 5_3:+54725522 None:intergenic
GAGGGTGAAGAAGCAGATCA+AGG 0.550519 5_3:-54725025 Msa0818710:CDS
TATACTCACTTTCCATGAGA+AGG 0.556322 5_3:+54723553 None:intergenic
ATCAAGGTGCCATTTGCGGT+GGG 0.557278 5_3:-54725009 Msa0818710:CDS
GGTGTGATATATTCAATGGA+AGG 0.558509 5_3:-54724972 Msa0818710:CDS
AAGGAAGAAAGAAATGAATG+AGG 0.563328 5_3:-54722566 Msa0818710:three_prime_UTR
TAGAAGGGTGAAAAGGTAAG+GGG 0.565912 5_3:+54725380 None:intergenic
GATCAAGGTGCCATTTGCGG+TGG 0.568008 5_3:-54725010 Msa0818710:CDS
CCGACAAAGATTACCAACAT+TGG 0.570402 5_3:-54724858 Msa0818710:CDS
AACAATGGACTCACCAGGTG+TGG 0.570501 5_3:+54725260 None:intergenic
GTATTTCAGTTATAGGAAAG+GGG 0.570896 5_3:+54722470 None:intergenic
GTGTGGAGAAGAATTTGTCT+TGG 0.571094 5_3:+54725277 None:intergenic
TTGAAACACTACGTGGAAAG+AGG 0.579791 5_3:-54724651 Msa0818710:CDS
CAGGAATGAGTTGATGGAGA+AGG 0.584159 5_3:+54724575 None:intergenic
ATTAATAATTCCACATACTG+GGG 0.584467 5_3:-54723003 Msa0818710:CDS
TATTTCAGTTATAGGAAAGG+GGG 0.584658 5_3:+54722471 None:intergenic
CCACTTAAGTGCCCAAAAGA+GGG 0.592767 5_3:-54725690 Msa0818710:five_prime_UTR
TCTTCCATTCTAAGAAGCAA+AGG 0.595126 5_3:-54725411 Msa0818710:CDS
AAAGGTTCAATCAATAAGCA+TGG 0.595162 5_3:-54723914 Msa0818710:CDS
ACAAAGATTACCAACATTGG+AGG 0.598469 5_3:-54724855 Msa0818710:CDS
GGATACATGTGACGATAACA+AGG 0.600528 5_3:-54722585 Msa0818710:three_prime_UTR
GTGACACTTTCATTTGTACA+CGG 0.608400 5_3:-54722647 Msa0818710:three_prime_UTR
ATGGAAGAAGGTGAGAATGG+TGG 0.608866 5_3:+54725426 None:intergenic
GTTGGAGACTCTCTCAATCG+TGG 0.612428 5_3:-54724619 Msa0818710:CDS
GTGGTTGCCACCTCCAATGT+TGG 0.612456 5_3:+54724845 None:intergenic
AATTGACTTGCCAAGAACAT+GGG 0.614611 5_3:-54724885 Msa0818710:CDS
CACGGAAAAGAGTATAGCTA+CGG 0.614974 5_3:-54722629 Msa0818710:three_prime_UTR
AAGATTACCAACATTGGAGG+TGG 0.615305 5_3:-54724852 Msa0818710:CDS
TATGAAAAGTATGCTTAGAT+GGG 0.615716 5_3:-54723629 Msa0818710:CDS
AATTAGTTATACGTATATGA+GGG 0.618030 5_3:-54725714 Msa0818710:five_prime_UTR
TCAAGGTGCCATTTGCGGTG+GGG 0.620684 5_3:-54725008 Msa0818710:CDS
TATGGCAGGAGTATAGATTG+GGG 0.621719 5_3:-54723066 Msa0818710:intron
AAGCATGGTAGAAATTGGAA+AGG 0.640034 5_3:-54723899 Msa0818710:CDS
CATTGGAGGTGGCAACCACA+CGG 0.642871 5_3:-54724841 Msa0818710:CDS
TCTCCATCAACTCATTCCTG+AGG 0.645543 5_3:-54724572 Msa0818710:CDS
AAAGTATGCTTAGATGGGTG+AGG 0.645663 5_3:-54723624 Msa0818710:CDS
GATTGTCACAATTTCAACTG+AGG 0.649875 5_3:-54723668 Msa0818710:CDS
GCTATGCTGATTGTGTACAT+TGG 0.671303 5_3:-54722804 Msa0818710:CDS
GTATATTAGGAGAAAGTGGG+AGG 0.692881 5_3:+54725579 None:intergenic
GGCAGGAGTATAGATTGGGG+AGG 0.697427 5_3:-54723063 Msa0818710:intron
TTAGGAAGATCGCAACCGTG+TGG 0.699752 5_3:+54724826 None:intergenic
CGGTGGGGAAGACAGAGGAA+GGG 0.705520 5_3:-54724993 Msa0818710:CDS
GATTGGGGAGGTGAGCCAGG+AGG 0.708567 5_3:-54723051 Msa0818710:CDS
ATTTGCGGTGGGGAAGACAG+AGG 0.711107 5_3:-54724998 Msa0818710:CDS
GGATACACGACCATTGTACG+AGG 0.724636 5_3:-54724935 Msa0818710:CDS
TACCCAATAAGACATCACAA+TGG 0.732110 5_3:+54725663 None:intergenic
TTGATGCTTGAAACACTACG+TGG 0.805901 5_3:-54724658 Msa0818710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAATAATTATATTTAG+TGG + chr5_3:54723936-54723955 None:intergenic 10.0%
!!! TAATTTTCAAATAATCAAAA+TGG + chr5_3:54724314-54724333 None:intergenic 10.0%
!! TATTATAGATCAATTTAAAT+AGG + chr5_3:54724624-54724643 None:intergenic 10.0%
!! ATTTAAATTGATCTATAATA+TGG - chr5_3:54724624-54724643 Msa0818710:CDS 10.0%
!! TTAAAATCTTATGAATATGT+CGG + chr5_3:54722933-54722952 None:intergenic 15.0%
!!! AATATATTATGCCTTTTTTA+TGG - chr5_3:54722976-54722995 Msa0818710:CDS 15.0%
!!! TTTATTTGATGTTTTGTATT+TGG - chr5_3:54722999-54723018 Msa0818710:CDS 15.0%
!!! TTATTTGATGTTTTGTATTT+GGG - chr5_3:54723000-54723019 Msa0818710:CDS 15.0%
!! TTCACTTTAATGTAATAATT+TGG - chr5_3:54723799-54723818 Msa0818710:intron 15.0%
!! TGAAAATCAACAATAAAAAA+TGG + chr5_3:54724020-54724039 None:intergenic 15.0%
!! AAGTCTAAAAAAATTGAAAA+GGG + chr5_3:54724352-54724371 None:intergenic 15.0%
!!! AATTGTAGAGATTTTTTTTA+AGG + chr5_3:54724730-54724749 None:intergenic 15.0%
!!! AAAAAAAATCTCTACAATTT+TGG - chr5_3:54724731-54724750 Msa0818710:intron 15.0%
!! ATAACAAAACTTTATTCTTT+AGG - chr5_3:54724816-54724835 Msa0818710:intron 15.0%
!! CTAATATTTCAACAAATATA+TGG - chr5_3:54724982-54725001 Msa0818710:CDS 15.0%
!! TGAATTATCTATATATTGTT+AGG - chr5_3:54725686-54725705 Msa0818710:five_prime_UTR 15.0%
!!! TTTTAACTATTAGTGTTTTT+TGG + chr5_3:54725728-54725747 None:intergenic 15.0%
!! AAATTAGTTATACGTATATG+AGG - chr5_3:54722351-54722370 Msa0818710:three_prime_UTR 20.0%
!! AATTAGTTATACGTATATGA+GGG - chr5_3:54722352-54722371 Msa0818710:three_prime_UTR 20.0%
!! ATTGAATGTACTAGTATATT+AGG + chr5_3:54722503-54722522 None:intergenic 20.0%
!!! TTTTACATCAAAAAGTATAG+AGG - chr5_3:54722866-54722885 Msa0818710:CDS 20.0%
!! TAAAATCTTATGAATATGTC+GGG + chr5_3:54722932-54722951 None:intergenic 20.0%
!! CATCAAATAAACCATAAAAA+AGG + chr5_3:54722990-54723009 None:intergenic 20.0%
!!! TATTTGATGTTTTGTATTTG+GGG - chr5_3:54723001-54723020 Msa0818710:CDS 20.0%
!! GCATAAATAAATACTTACTT+AGG + chr5_3:54723261-54723280 None:intergenic 20.0%
!! TTCATAACAATAATGAAGAT+CGG + chr5_3:54723587-54723606 None:intergenic 20.0%
!! TTCATTATTGTTATGAATGA+TGG - chr5_3:54723590-54723609 Msa0818710:CDS 20.0%
!! AGTTAATTATAAGAGTGAAT+CGG - chr5_3:54723735-54723754 Msa0818710:intron 20.0%
!! CAAATAATCAAAATGGAAAA+GGG + chr5_3:54724307-54724326 None:intergenic 20.0%
!! TCAAATAATCAAAATGGAAA+AGG + chr5_3:54724308-54724327 None:intergenic 20.0%
!! CAAGTCTAAAAAAATTGAAA+AGG + chr5_3:54724353-54724372 None:intergenic 20.0%
!!! CTTTTCAATTTTTTTAGACT+TGG - chr5_3:54724351-54724370 Msa0818710:intron 20.0%
!! ACACATTTGTTCAAATATAA+AGG - chr5_3:54725355-54725374 Msa0818710:CDS 20.0%
!! CACATTTGTTCAAATATAAA+GGG - chr5_3:54725356-54725375 Msa0818710:CDS 20.0%
!!! ACTACAAAAAATTTGGAATT+TGG + chr5_3:54725621-54725640 None:intergenic 20.0%
!!! TCCAAATTTTTTGTAGTTTT+TGG - chr5_3:54725624-54725643 Msa0818710:five_prime_UTR 20.0%
!! ACCAAAAACTACAAAAAATT+TGG + chr5_3:54725628-54725647 None:intergenic 20.0%
!! TAAATAATAGTGAGTTGAGT+TGG - chr5_3:54722589-54722608 Msa0818710:three_prime_UTR 25.0%
! AAAAAGAATGAAAGCTAGAA+GGG + chr5_3:54722704-54722723 None:intergenic 25.0%
!! CTTTTTGTTACTATTCTCTA+TGG - chr5_3:54722718-54722737 Msa0818710:three_prime_UTR 25.0%
!!! TGTAGCATTTTTTGAAACAA+TGG + chr5_3:54722824-54722843 None:intergenic 25.0%
! AAAATCTTATGAATATGTCG+GGG + chr5_3:54722931-54722950 None:intergenic 25.0%
!! ATTTGATGTTTTGTATTTGG+GGG - chr5_3:54723002-54723021 Msa0818710:CDS 25.0%
! TTGATAAAAGAACGAGATAA+TGG - chr5_3:54723702-54723721 Msa0818710:intron 25.0%
!! TGATTTTCATGTGTTTAATC+AGG - chr5_3:54724031-54724050 Msa0818710:intron 25.0%
!!! TTTTCTTCTAGAATCAAACT+CGG - chr5_3:54724088-54724107 Msa0818710:intron 25.0%
! TCTGATAGAATTGTAAGAAA+AGG - chr5_3:54724134-54724153 Msa0818710:intron 25.0%
!!! ATCTTTTGATGATGAAGTAA+AGG - chr5_3:54724374-54724393 Msa0818710:intron 25.0%
!! TATGAAAAGTATGCTTAGAT+GGG - chr5_3:54724437-54724456 Msa0818710:intron 25.0%
!!! AGTTTTGTTATAATAGTGAC+CGG + chr5_3:54724807-54724826 None:intergenic 25.0%
!! ATACGCAAAATTTCACATTT+TGG + chr5_3:54724905-54724924 None:intergenic 25.0%
! GAAATATTAGAACTTAGAAG+TGG + chr5_3:54724972-54724991 None:intergenic 25.0%
! TATTTCAACAAATATATGGC+AGG - chr5_3:54724986-54725005 Msa0818710:CDS 25.0%
! TGATTAATAATTCCACATAC+TGG - chr5_3:54725061-54725080 Msa0818710:intron 25.0%
! GATTAATAATTCCACATACT+GGG - chr5_3:54725062-54725081 Msa0818710:intron 25.0%
! ATTAATAATTCCACATACTG+GGG - chr5_3:54725063-54725082 Msa0818710:intron 25.0%
!! AAAAGTATAATGCAAGTGAT+TGG - chr5_3:54725099-54725118 Msa0818710:intron 25.0%
! GTGATGTTAAGAAATGTAAT+TGG + chr5_3:54725150-54725169 None:intergenic 25.0%
! CTAATTGCTCTTTAGTTAAA+GGG + chr5_3:54725230-54725249 None:intergenic 25.0%
!! ACAAATGTGTACAATTTTCA+AGG + chr5_3:54725345-54725364 None:intergenic 25.0%
!! ATCATATATTGTTGTTCTCT+TGG - chr5_3:54725379-54725398 Msa0818710:CDS 25.0%
!! TGATTAATCTTTGTTTGCAA+AGG - chr5_3:54725460-54725479 Msa0818710:exon 25.0%
! GAATTTGGTATTTCAGTTAT+AGG + chr5_3:54725606-54725625 None:intergenic 25.0%
TGAACTTATGTCTTTGTTGA+TGG + chr5_3:54722455-54722474 None:intergenic 30.0%
CTAGTATATTAGGAGAAAGT+GGG + chr5_3:54722493-54722512 None:intergenic 30.0%
ACTAGTATATTAGGAGAAAG+TGG + chr5_3:54722494-54722513 None:intergenic 30.0%
! GTACTTTACTCTAAAAGACT+TGG + chr5_3:54722547-54722566 None:intergenic 30.0%
CAAAAAGAATGAAAGCTAGA+AGG + chr5_3:54722705-54722724 None:intergenic 30.0%
! TTTGATGTTTTGTATTTGGG+GGG - chr5_3:54723003-54723022 Msa0818710:CDS 30.0%
TGATATATTCAATGGAAGGT+GGG - chr5_3:54723098-54723117 Msa0818710:intron 30.0%
GAAAACAGTTAGTGAATCAA+AGG + chr5_3:54723538-54723557 None:intergenic 30.0%
AGAAATCATAAGTATTCGTG+TGG - chr5_3:54723649-54723668 Msa0818710:CDS 30.0%
GAAATCATAAGTATTCGTGT+GGG - chr5_3:54723650-54723669 Msa0818710:CDS 30.0%
AATCAACTAGAGTTAGTTGT+TGG + chr5_3:54723781-54723800 None:intergenic 30.0%
ATGCAACAATTGAGTTCTAT+TGG - chr5_3:54724060-54724079 Msa0818710:intron 30.0%
TGCAACAATTGAGTTCTATT+GGG - chr5_3:54724061-54724080 Msa0818710:intron 30.0%
! GAGTTTGATTCTAGAAGAAA+AGG + chr5_3:54724089-54724108 None:intergenic 30.0%
AAAGGTTCAATCAATAAGCA+TGG - chr5_3:54724152-54724171 Msa0818710:intron 30.0%
TCAATAAGCATGGTAGAAAT+TGG - chr5_3:54724162-54724181 Msa0818710:intron 30.0%
TGGTATTCAACACTTATCTT+TGG - chr5_3:54724201-54724220 Msa0818710:intron 30.0%
TATTCAACACTTATCTTTGG+TGG - chr5_3:54724204-54724223 Msa0818710:intron 30.0%
GCTTGTTTGAATAGAAAAGA+TGG + chr5_3:54724263-54724282 None:intergenic 30.0%
! CTATGAAAAGTATGCTTAGA+TGG - chr5_3:54724436-54724455 Msa0818710:intron 30.0%
!!! AAGAAGACTCTAGTTTTCTT+TGG + chr5_3:54724480-54724499 None:intergenic 30.0%
! GAAACCAAACCATACAATTT+TGG - chr5_3:54724701-54724720 Msa0818710:intron 30.0%
! TTAAGGTGTCCAAAATTGTA+TGG + chr5_3:54724713-54724732 None:intergenic 30.0%
TGCGTATAATAATGATCAGA+AGG - chr5_3:54724918-54724937 Msa0818710:CDS 30.0%
CTTCAATCTATAAGAAGCAA+TGG - chr5_3:54725202-54725221 Msa0818710:intron 30.0%
GCTAATTGCTCTTTAGTTAA+AGG + chr5_3:54725231-54725250 None:intergenic 30.0%
AAGGAAGAAAGAAATGAATG+AGG - chr5_3:54725500-54725519 Msa0818710:five_prime_UTR 30.0%
GTTAGTAGTTGTTCAAAGAA+AGG - chr5_3:54725536-54725555 Msa0818710:five_prime_UTR 30.0%
TATTTCAGTTATAGGAAAGG+GGG + chr5_3:54725598-54725617 None:intergenic 30.0%
GTATTTCAGTTATAGGAAAG+GGG + chr5_3:54725599-54725618 None:intergenic 30.0%
GGTATTTCAGTTATAGGAAA+GGG + chr5_3:54725600-54725619 None:intergenic 30.0%
TGGTATTTCAGTTATAGGAA+AGG + chr5_3:54725601-54725620 None:intergenic 30.0%
ACTCCATTGTGATGTCTTAT+TGG - chr5_3:54722400-54722419 Msa0818710:three_prime_UTR 35.0%
! CTCCATTGTGATGTCTTATT+GGG - chr5_3:54722401-54722420 Msa0818710:three_prime_UTR 35.0%
TACCCAATAAGACATCACAA+TGG + chr5_3:54722406-54722425 None:intergenic 35.0%
! TTGTGATGTCTTATTGGGTA+AGG - chr5_3:54722406-54722425 Msa0818710:three_prime_UTR 35.0%
!! ATAGTGAGTTGAGTTGGATT+TGG - chr5_3:54722595-54722614 Msa0818710:three_prime_UTR 35.0%
TTGCTTCTTAGAATGGAAGA+AGG + chr5_3:54722655-54722674 None:intergenic 35.0%
! TCTTCCATTCTAAGAAGCAA+AGG - chr5_3:54722655-54722674 Msa0818710:three_prime_UTR 35.0%
GAAGATTTCATGTGCATCTT+TGG - chr5_3:54722742-54722761 Msa0818710:CDS 35.0%
GGGTGAATAATGAAAAGAGA+GGG - chr5_3:54723023-54723042 Msa0818710:CDS 35.0%
! GAAGGGTGTGATATATTCAA+TGG - chr5_3:54723090-54723109 Msa0818710:intron 35.0%
GGTGTGATATATTCAATGGA+AGG - chr5_3:54723094-54723113 Msa0818710:intron 35.0%
GTGATATATTCAATGGAAGG+TGG - chr5_3:54723097-54723116 Msa0818710:intron 35.0%
GTCAATTGAGGTTGTATGTA+CGG + chr5_3:54723168-54723187 None:intergenic 35.0%
!! AATTGACTTGCCAAGAACAT+GGG - chr5_3:54723181-54723200 Msa0818710:intron 35.0%
!! CAATGTTGGTAATCTTTGTC+GGG + chr5_3:54723210-54723229 None:intergenic 35.0%
!! CCAATGTTGGTAATCTTTGT+CGG + chr5_3:54723211-54723230 None:intergenic 35.0%
ACAAAGATTACCAACATTGG+AGG - chr5_3:54723211-54723230 Msa0818710:intron 35.0%
ATCAAAGGTTTCCATGGATT+TGG + chr5_3:54723523-54723542 None:intergenic 35.0%
TAGTGAATCAAAGGTTTCCA+TGG + chr5_3:54723529-54723548 None:intergenic 35.0%
! ACTAACTGTTTTCACAGCAA+AGG - chr5_3:54723545-54723564 Msa0818710:intron 35.0%
! ACTAGAGTTAGTTGTTGGTT+AGG + chr5_3:54723776-54723795 None:intergenic 35.0%
AAGCATGGTAGAAATTGGAA+AGG - chr5_3:54724167-54724186 Msa0818710:intron 35.0%
!!! TTGGAAAGGTGTTGACATTT+TGG - chr5_3:54724181-54724200 Msa0818710:intron 35.0%
GATTGTCACAATTTCAACTG+AGG - chr5_3:54724398-54724417 Msa0818710:intron 35.0%
TATACTCACTTTCCATGAGA+AGG + chr5_3:54724516-54724535 None:intergenic 35.0%
GTGTCCAAAATTGTATGGTT+TGG + chr5_3:54724708-54724727 None:intergenic 35.0%
! ACATGCTCGTTGTTAATTTG+AGG + chr5_3:54724777-54724796 None:intergenic 35.0%
TTGTTATAATAGTGACCGGA+GGG + chr5_3:54724803-54724822 None:intergenic 35.0%
! TTTGTTATAATAGTGACCGG+AGG + chr5_3:54724804-54724823 None:intergenic 35.0%
! TATATGGCAGGAGTATAGAT+TGG - chr5_3:54724998-54725017 Msa0818710:CDS 35.0%
! ATATGGCAGGAGTATAGATT+GGG - chr5_3:54724999-54725018 Msa0818710:CDS 35.0%
TTGAAGTGTGTGCATCTTTA+CGG + chr5_3:54725188-54725207 None:intergenic 35.0%
! TACCTGGTCTTCAAGATAAT+TGG - chr5_3:54725289-54725308 Msa0818710:CDS 35.0%
TTCCAATTATCTTGAAGACC+AGG + chr5_3:54725294-54725313 None:intergenic 35.0%
! ATTGTTGTTCTCTTGGATCA+TGG - chr5_3:54725386-54725405 Msa0818710:CDS 35.0%
GTGACACTTTCATTTGTACA+CGG - chr5_3:54725419-54725438 Msa0818710:CDS 35.0%
! ACAATGGAGTTCCCTCTTTT+GGG + chr5_3:54722390-54722409 None:intergenic 40.0%
GTATATTAGGAGAAAGTGGG+AGG + chr5_3:54722490-54722509 None:intergenic 40.0%
AGAATGGAAGAAGGTGAGAA+TGG + chr5_3:54722646-54722665 None:intergenic 40.0%
ACGACCTTTGCTTCTTAGAA+TGG + chr5_3:54722662-54722681 None:intergenic 40.0%
TAGAAGGGTGAAAAGGTAAG+GGG + chr5_3:54722689-54722708 None:intergenic 40.0%
CTAGAAGGGTGAAAAGGTAA+GGG + chr5_3:54722690-54722709 None:intergenic 40.0%
TGAAAGCTAGAAGGGTGAAA+AGG + chr5_3:54722696-54722715 None:intergenic 40.0%
GTGTGGAGAAGAATTTGTCT+TGG + chr5_3:54722792-54722811 None:intergenic 40.0%
! TTTGAAACAATGGACTCACC+AGG + chr5_3:54722814-54722833 None:intergenic 40.0%
GCGAGTTTGAACTCTCTTAT+GGG + chr5_3:54722907-54722926 None:intergenic 40.0%
GGGGTGAATAATGAAAAGAG+AGG - chr5_3:54723022-54723041 Msa0818710:CDS 40.0%
! TATTCAATGGAAGGTGGGTT+TGG - chr5_3:54723103-54723122 Msa0818710:intron 40.0%
! ATTCAATGGAAGGTGGGTTT+GGG - chr5_3:54723104-54723123 Msa0818710:intron 40.0%
TGTTCTTGGCAAGTCAATTG+AGG + chr5_3:54723180-54723199 None:intergenic 40.0%
!! CAATTGACTTGCCAAGAACA+TGG - chr5_3:54723180-54723199 Msa0818710:intron 40.0%
CCGACAAAGATTACCAACAT+TGG - chr5_3:54723208-54723227 Msa0818710:intron 40.0%
AAGATTACCAACATTGGAGG+TGG - chr5_3:54723214-54723233 Msa0818710:intron 40.0%
TTGATGCTTGAAACACTACG+TGG - chr5_3:54723408-54723427 Msa0818710:intron 40.0%
TTGAAACACTACGTGGAAAG+AGG - chr5_3:54723415-54723434 Msa0818710:intron 40.0%
GGAAAGAGGATGATGTTTGT+TGG - chr5_3:54723429-54723448 Msa0818710:intron 40.0%
TAGAGATCATAGTGATCAGC+AGG - chr5_3:54723624-54723643 Msa0818710:CDS 40.0%
! ACTTATCTTTGGTGGATGAC+TGG - chr5_3:54724212-54724231 Msa0818710:intron 40.0%
AACTGAGGATGCTTATCGTA+TGG - chr5_3:54724413-54724432 Msa0818710:intron 40.0%
AAAGTATGCTTAGATGGGTG+AGG - chr5_3:54724442-54724461 Msa0818710:intron 40.0%
! TATGGCAGGAGTATAGATTG+GGG - chr5_3:54725000-54725019 Msa0818710:CDS 40.0%
TCATTGTAACAACTTCCTCC+TGG + chr5_3:54725033-54725052 None:intergenic 40.0%
CACAATCAGCATAGCTTACA+GGG + chr5_3:54725257-54725276 None:intergenic 40.0%
ACACAATCAGCATAGCTTAC+AGG + chr5_3:54725258-54725277 None:intergenic 40.0%
! GCTATGCTGATTGTGTACAT+TGG - chr5_3:54725262-54725281 Msa0818710:intron 40.0%
! TGTGTACATTGGTGTTTACC+TGG - chr5_3:54725273-54725292 Msa0818710:intron 40.0%
! CACGGAAAAGAGTATAGCTA+CGG - chr5_3:54725437-54725456 Msa0818710:CDS 40.0%
GGATACATGTGACGATAACA+AGG - chr5_3:54725481-54725500 Msa0818710:five_prime_UTR 40.0%
ACCACTTAAGTGCCCAAAAG+AGG - chr5_3:54722375-54722394 Msa0818710:three_prime_UTR 45.0%
CCACTTAAGTGCCCAAAAGA+GGG - chr5_3:54722376-54722395 Msa0818710:three_prime_UTR 45.0%
! CACAATGGAGTTCCCTCTTT+TGG + chr5_3:54722391-54722410 None:intergenic 45.0%
ATGGAAGAAGGTGAGAATGG+TGG + chr5_3:54722643-54722662 None:intergenic 45.0%
GCTAGAAGGGTGAAAAGGTA+AGG + chr5_3:54722691-54722710 None:intergenic 45.0%
GACAAATTCTTCTCCACACC+TGG - chr5_3:54722793-54722812 Msa0818710:CDS 45.0%
CGCGAGTTTGAACTCTCTTA+TGG + chr5_3:54722908-54722927 None:intergenic 45.0%
ACGACCATTGTACGAGGAAT+CGG - chr5_3:54723137-54723156 Msa0818710:intron 45.0%
!! CAGGAATGAGTTGATGGAGA+AGG + chr5_3:54723494-54723513 None:intergenic 45.0%
TCTCCATCAACTCATTCCTG+AGG - chr5_3:54723494-54723513 Msa0818710:intron 45.0%
! CATCCTCAGGAATGAGTTGA+TGG + chr5_3:54723500-54723519 None:intergenic 45.0%
CGGACAATGCTGTTGTTCAT+AGG - chr5_3:54724108-54724127 Msa0818710:intron 45.0%
ACTAGCATGTCACCTTCTCA+TGG - chr5_3:54724501-54724520 Msa0818710:intron 45.0%
ACAATCAGAACCCCAGTATG+TGG + chr5_3:54725076-54725095 None:intergenic 45.0%
TCTCTTGGATCATGGACTCT+TGG - chr5_3:54725394-54725413 Msa0818710:CDS 45.0%
CTCTTGGATCATGGACTCTT+GGG - chr5_3:54725395-54725414 Msa0818710:CDS 45.0%
! CCCTCTTTTGGGCACTTAAG+TGG + chr5_3:54722379-54722398 None:intergenic 50.0%
! AACAATGGACTCACCAGGTG+TGG + chr5_3:54722809-54722828 None:intergenic 50.0%
GAGGGTGAAGAAGCAGATCA+AGG - chr5_3:54723041-54723060 Msa0818710:CDS 50.0%
!! GCAGATCAAGGTGCCATTTG+CGG - chr5_3:54723053-54723072 Msa0818710:CDS 50.0%
!! ATCAAGGTGCCATTTGCGGT+GGG - chr5_3:54723057-54723076 Msa0818710:CDS 50.0%
GGATACACGACCATTGTACG+AGG - chr5_3:54723131-54723150 Msa0818710:intron 50.0%
CACTCCGATTCCTCGTACAA+TGG + chr5_3:54723144-54723163 None:intergenic 50.0%
TTAGGAAGATCGCAACCGTG+TGG + chr5_3:54723243-54723262 None:intergenic 50.0%
GTTGGAGACTCTCTCAATCG+TGG - chr5_3:54723447-54723466 Msa0818710:intron 50.0%
TCCTGAGGATGCCAAATCCA+TGG - chr5_3:54723509-54723528 Msa0818710:intron 50.0%
TCCATGGATTTGGCATCCTC+AGG + chr5_3:54723513-54723532 None:intergenic 50.0%
GTTAACACGCGGAGGATACT+TGG + chr5_3:54723831-54723850 None:intergenic 50.0%
CGAGCAACAACGTTAACACG+CGG + chr5_3:54723842-54723861 None:intergenic 50.0%
! TCTTTGGTGGATGACTGGCT+TGG - chr5_3:54724217-54724236 Msa0818710:intron 50.0%
!! GATCAAGGTGCCATTTGCGG+TGG - chr5_3:54723056-54723075 Msa0818710:CDS 55.0%
!! TCAAGGTGCCATTTGCGGTG+GGG - chr5_3:54723058-54723077 Msa0818710:CDS 55.0%
TCTGTCTTCCCCACCGCAAA+TGG + chr5_3:54723069-54723088 None:intergenic 55.0%
ATTTGCGGTGGGGAAGACAG+AGG - chr5_3:54723068-54723087 Msa0818710:intron 55.0%
TGTCGGGTCTCCCATGTTCT+TGG + chr5_3:54723194-54723213 None:intergenic 55.0%
GTGGTTGCCACCTCCAATGT+TGG + chr5_3:54723224-54723243 None:intergenic 55.0%
CATTGGAGGTGGCAACCACA+CGG - chr5_3:54723225-54723244 Msa0818710:intron 55.0%
GCAACAACGTTAACACGCGG+AGG + chr5_3:54723839-54723858 None:intergenic 55.0%
CAACGAGCATGTACACCCTC+CGG - chr5_3:54724785-54724804 Msa0818710:intron 55.0%
! GGCAGGAGTATAGATTGGGG+AGG - chr5_3:54725003-54725022 Msa0818710:CDS 55.0%
ATAGATTGGGGAGGTGAGCC+AGG - chr5_3:54725012-54725031 Msa0818710:CDS 55.0%
CGGTGGGGAAGACAGAGGAA+GGG - chr5_3:54723073-54723092 Msa0818710:intron 60.0%
! TGGAAGGTGGGTTTGGGACG+AGG - chr5_3:54723110-54723129 Msa0818710:intron 60.0%
GCGGTGGGGAAGACAGAGGA+AGG - chr5_3:54723072-54723091 Msa0818710:intron 65.0%
GATTGGGGAGGTGAGCCAGG+AGG - chr5_3:54725015-54725034 Msa0818710:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 54722336 54725752 54722336 ID=Msa0818710;Name=Msa0818710
chr5_3 mRNA 54722336 54725752 54722336 ID=Msa0818710-mRNA-1;Parent=Msa0818710;Name=Msa0818710-mRNA-1;_AED=0.05;_eAED=0.05;_QI=289|1|1|1|1|1|6|405|427
chr5_3 exon 54722336 54723080 54722336 ID=Msa0818710-mRNA-1:exon:16607;Parent=Msa0818710-mRNA-1
chr5_3 exon 54723562 54723720 54723562 ID=Msa0818710-mRNA-1:exon:16606;Parent=Msa0818710-mRNA-1
chr5_3 exon 54723836 54724035 54723836 ID=Msa0818710-mRNA-1:exon:16605;Parent=Msa0818710-mRNA-1
chr5_3 exon 54724522 54724693 54724522 ID=Msa0818710-mRNA-1:exon:16604;Parent=Msa0818710-mRNA-1
chr5_3 exon 54724826 54725048 54724826 ID=Msa0818710-mRNA-1:exon:16603;Parent=Msa0818710-mRNA-1
chr5_3 exon 54725274 54725752 54725274 ID=Msa0818710-mRNA-1:exon:16602;Parent=Msa0818710-mRNA-1
chr5_3 five_prime_UTR 54725464 54725752 54725464 ID=Msa0818710-mRNA-1:five_prime_utr;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54725274 54725463 54725274 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54724826 54725048 54724826 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54724522 54724693 54724522 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54723836 54724035 54723836 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54723562 54723720 54723562 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 CDS 54722741 54723080 54722741 ID=Msa0818710-mRNA-1:cds;Parent=Msa0818710-mRNA-1
chr5_3 three_prime_UTR 54722336 54722740 54722336 ID=Msa0818710-mRNA-1:three_prime_utr;Parent=Msa0818710-mRNA-1
Gene Sequence

>Msa0818710

ATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCATTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGAGTATAGATTGGGGAGGTGAGCCAGGAGGAAGTTGTTACAATGAAACAACTTTGATTAATAATTCCACATACTGGGGTTCTGATTGTAGAAAAAGTATAATGCAAGTGATTGGTGAAGTGTTAAGCAAAACAAAAGTTCCAATTACATTTCTTAACATCACACAACTCTCAAGTTACCGTAAAGATGCACACACTTCAATCTATAAGAAGCAATGGAGCCCTTTAACTAAAGAGCAATTAGCTAACCCTGTAAGCTATGCTGATTGTGTACATTGGTGTTTACCTGGTCTTCAAGATAATTGGAATGAACTTTTGTTTGCTAAGTTGTTTTATCCTTGA
Protein sequence

>Msa0818710

MQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGKSIDWGGEPGGSCYNETTLINNSTYWGSDCRKSIMQVIGEVLSKTKVPITFLNITQLSSYRKDAHTSIYKKQWSPLTKEQLANPVSYADCVHWCLPGLQDNWNELLFAKLFYP*