AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1170830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1170830 MtrunA17_Chr5g0437601 99.297 427 3 0 1 427 1 427 0.0 890
Msa1170830 MtrunA17_Chr3g0114101 74.596 433 101 5 4 427 1 433 0.0 660
Msa1170830 MtrunA17_Chr4g0005491 51.003 349 165 3 83 426 62 409 2.44e-132 387
Msa1170830 MtrunA17_Chr7g0243301 48.677 378 183 5 53 425 39 410 4.74e-132 386
Msa1170830 MtrunA17_Chr4g0005481 52.187 343 161 2 79 421 104 443 3.05e-129 380
Msa1170830 MtrunA17_Chr4g0006991 48.986 345 174 2 81 424 84 427 9.86e-129 378
Msa1170830 MtrunA17_Chr8g0360521 49.025 359 175 4 71 425 56 410 5.42e-128 376
Msa1170830 MtrunA17_Chr3g0113671 51.289 349 164 4 80 424 103 449 1.25e-126 374
Msa1170830 MtrunA17_Chr5g0437291 48.571 350 175 3 81 425 163 512 9.06e-119 356
Msa1170830 MtrunA17_Chr7g0242701 41.647 425 238 4 10 424 3 427 1.09e-118 352
Msa1170830 MtrunA17_Chr5g0430371 36.675 379 207 9 64 426 40 401 1.24e-76 244
Msa1170830 MtrunA17_Chr3g0105301 36.944 360 201 8 76 426 36 378 1.57e-76 243
Msa1170830 MtrunA17_Chr5g0413271 36.932 352 206 8 77 425 139 477 2.08e-75 243
Msa1170830 MtrunA17_Chr4g0041011 34.097 393 226 9 32 424 4 363 3.49e-75 239
Msa1170830 MtrunA17_Chr1g0210771 38.329 347 186 10 83 424 59 382 1.11e-74 238
Msa1170830 MtrunA17_Chr2g0325481 37.026 343 191 7 83 425 50 367 2.62e-74 236
Msa1170830 MtrunA17_Chr1g0175671 36.723 354 200 8 83 425 170 510 1.90e-73 239
Msa1170830 MtrunA17_Chr3g0097891 35.127 353 203 8 75 425 23 351 1.39e-72 231
Msa1170830 MtrunA17_Chr7g0258851 37.255 357 194 9 83 425 248 588 1.79e-72 238
Msa1170830 MtrunA17_Chr1g0171171 33.668 398 234 10 27 424 8 375 6.79e-72 231
Msa1170830 MtrunA17_Chr7g0259711 33.034 445 258 15 2 425 8 433 1.59e-71 232
Msa1170830 MtrunA17_Chr4g0071391 37.143 350 200 8 83 424 109 446 2.05e-71 232
Msa1170830 MtrunA17_Chr2g0287901 37.183 355 196 11 81 422 95 435 5.72e-70 228
Msa1170830 MtrunA17_Chr2g0314801 36.286 350 192 8 77 421 27 350 9.66e-69 222
Msa1170830 MtrunA17_Chr2g0283581 34.925 398 211 11 27 424 9 358 2.57e-68 221
Msa1170830 MtrunA17_Chr7g0269991 36.648 352 192 9 83 424 137 467 5.71e-68 223
Msa1170830 MtrunA17_Chr3g0085081 35.817 349 190 10 81 424 29 348 1.29e-67 219
Msa1170830 MtrunA17_Chr5g0413851 37.644 348 189 10 79 424 38 359 1.54e-67 219
Msa1170830 MtrunA17_Chr4g0045941 33.903 351 208 8 75 425 25 351 1.55e-66 216
Msa1170830 MtrunA17_Chr3g0085071 35.817 349 190 10 81 424 44 363 2.31e-66 216
Msa1170830 MtrunA17_Chr1g0213281 33.943 383 231 10 56 426 145 517 2.39e-66 220
Msa1170830 MtrunA17_Chr4g0068111 33.711 353 212 7 83 425 114 454 7.10e-66 218
Msa1170830 MtrunA17_Chr3g0091031 35.244 349 192 10 81 424 29 348 2.71e-65 213
Msa1170830 MtrunA17_Chr8g0368461 33.333 372 213 10 76 425 171 529 3.28e-63 213
Msa1170830 MtrunA17_Chr4g0045951 33.333 348 208 8 78 425 40 363 1.50e-61 203
Msa1170830 MtrunA17_Chr2g0314791 35.393 356 185 13 80 425 26 346 2.01e-60 200
Msa1170830 MtrunA17_Chr8g0384151 28.440 436 260 13 5 425 19 417 6.83e-55 188
Msa1170830 MtrunA17_Chr3g0126021 29.609 358 219 8 79 425 125 460 2.06e-52 182
Msa1170830 MtrunA17_Chr6g0456681 30.833 360 220 10 81 424 62 408 3.83e-43 156
Msa1170830 MtrunA17_Chr1g0209921 34.043 235 143 6 81 313 42 266 3.91e-42 149
Msa1170830 MtrunA17_Chr1g0188191 27.889 398 254 16 43 421 195 578 4.66e-40 150
Msa1170830 MtrunA17_Chr1g0178081 29.261 352 180 9 81 425 53 342 5.44e-40 146
Msa1170830 MtrunA17_Chr6g0456691 27.419 434 262 16 16 424 14 419 3.69e-39 145
Msa1170830 MtrunA17_Chr8g0347321 28.571 357 228 12 83 424 95 439 6.88e-39 145
Msa1170830 MtrunA17_Chr8g0384421 30.028 363 214 13 81 424 131 472 2.46e-37 141
Msa1170830 MtrunA17_Chr6g0476041 28.011 357 231 14 81 421 165 511 3.29e-37 142
Msa1170830 MtrunA17_Chr8g0391481 29.428 367 219 15 80 424 24 372 2.38e-36 137
Msa1170830 MtrunA17_Chr6g0451321 28.032 371 229 14 73 424 106 457 5.76e-36 137
Msa1170830 MtrunA17_Chr5g0414061 27.887 355 227 12 83 421 94 435 4.22e-35 135
Msa1170830 MtrunA17_Chr5g0414091 28.453 362 222 12 80 420 82 427 8.64e-35 134
Msa1170830 MtrunA17_Chr2g0283851 27.559 381 230 15 65 420 37 396 8.87e-35 133
Msa1170830 MtrunA17_Chr5g0414051 25.886 367 226 13 80 421 72 417 4.38e-33 129
Msa1170830 MtrunA17_Chr3g0140371 28.333 360 223 13 80 421 15 357 9.76e-33 127
Msa1170830 MtrunA17_Chr7g0226991 27.348 362 227 14 80 421 54 399 1.43e-32 127
Msa1170830 MtrunA17_Chr8g0387921 26.479 355 231 12 83 420 74 415 2.71e-32 127
Msa1170830 MtrunA17_Chr5g0414041 28.571 357 224 12 83 421 87 430 1.44e-31 125
Msa1170830 MtrunA17_Chr4g0049421 26.997 363 218 16 83 420 48 388 1.69e-29 118
Msa1170830 MtrunA17_Chr6g0455901 26.503 366 231 12 80 424 68 416 9.03e-29 117
Msa1170830 MtrunA17_Chr4g0070341 27.704 379 202 16 81 424 130 471 2.22e-27 113
Msa1170830 MtrunA17_Chr7g0221271 40.000 130 77 1 81 210 77 205 4.19e-26 104
Msa1170830 MtrunA17_Chr7g0221291 45.361 97 52 1 79 175 75 170 1.71e-24 99.4
Msa1170830 MtrunA17_Chr2g0287931 36.134 119 66 4 143 252 1 118 1.77e-15 72.8
Msa1170830 MtrunA17_Chr3g0085101 38.318 107 62 3 280 385 5 108 6.20e-13 65.5
Msa1170830 MtrunA17_Chr7g0221281 25.311 241 155 10 197 424 8 236 7.06e-12 65.5
Msa1170830 MtrunA17_Chr2g0287911 43.750 64 28 2 76 139 54 109 1.23e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1170830 AT2G40320.1 78.537 410 86 1 17 426 17 424 0.0 689
Msa1170830 AT3G11030.1 62.723 448 137 4 8 427 6 451 0.0 588
Msa1170830 AT5G01620.2 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa1170830 AT5G01620.1 50.000 350 171 2 73 421 100 446 4.44e-129 380
Msa1170830 AT5G01620.3 50.000 350 171 2 73 421 108 454 1.00e-128 379
Msa1170830 AT3G55990.1 53.009 349 158 3 81 425 138 484 4.94e-128 379
Msa1170830 AT1G73140.1 44.444 378 193 3 49 425 45 406 7.02e-127 373
Msa1170830 AT5G01360.2 48.696 345 176 1 81 424 34 378 1.68e-126 371
Msa1170830 AT2G40150.1 51.233 365 163 4 73 424 58 420 5.92e-126 371
Msa1170830 AT5G01360.1 48.696 345 176 1 81 424 90 434 6.81e-126 371
Msa1170830 AT2G38320.1 44.865 370 198 3 61 425 37 405 8.04e-117 347
Msa1170830 AT2G40160.1 40.821 414 229 7 23 426 18 425 1.70e-107 324
Msa1170830 AT2G40160.2 41.176 408 224 7 23 420 18 419 4.32e-107 323
Msa1170830 AT2G38320.2 47.188 320 163 3 111 425 4 322 1.18e-105 316
Msa1170830 AT3G12060.1 38.259 379 204 9 61 425 174 536 3.80e-82 263
Msa1170830 AT5G06700.1 38.547 358 192 10 81 425 253 595 1.11e-78 255
Msa1170830 AT3G62390.1 37.166 374 203 11 68 426 117 473 1.97e-75 243
Msa1170830 AT1G78710.1 35.429 350 199 8 78 425 33 357 3.19e-75 239
Msa1170830 AT5G20590.1 36.639 363 209 8 72 425 130 480 3.68e-74 240
Msa1170830 AT2G40160.3 39.799 299 166 5 23 313 18 310 3.78e-74 234
Msa1170830 AT5G19160.1 34.651 430 237 14 20 422 30 442 5.21e-74 239
Msa1170830 AT3G14850.2 37.249 349 188 7 78 421 29 351 9.90e-73 233
Msa1170830 AT2G30900.1 35.135 370 204 12 68 426 23 367 4.13e-72 231
Msa1170830 AT3G06080.2 32.118 439 247 12 15 422 25 443 1.82e-70 230
Msa1170830 AT5G58600.1 35.411 353 203 7 83 426 66 402 2.22e-70 228
Msa1170830 AT5G49340.1 35.230 369 219 9 68 425 83 442 2.39e-70 229
Msa1170830 AT1G29050.1 34.582 347 203 6 78 424 56 378 4.46e-70 226
Msa1170830 AT2G30010.1 35.309 388 214 10 54 426 33 398 5.49e-70 227
Msa1170830 AT2G42570.1 34.140 372 217 9 54 425 22 365 1.17e-69 225
Msa1170830 AT2G30010.2 35.309 388 214 10 54 426 76 441 1.61e-69 227
Msa1170830 AT2G34070.1 34.473 351 199 8 78 424 60 383 2.36e-69 224
Msa1170830 AT2G31110.2 36.047 344 192 9 83 424 45 362 7.11e-69 223
Msa1170830 AT1G60790.1 34.551 356 212 9 81 425 188 533 2.02e-68 226
Msa1170830 AT3G54260.1 36.578 339 195 8 83 420 51 370 8.23e-67 218
Msa1170830 AT5G06230.2 34.426 366 196 11 83 425 21 365 3.10e-61 203
Msa1170830 AT5G06230.1 34.426 366 196 11 83 425 62 406 1.14e-60 203
Msa1170830 AT1G48880.1 30.095 422 232 14 23 425 63 440 4.01e-57 194
Msa1170830 AT1G78710.2 30.747 348 165 8 78 425 26 297 2.00e-55 186
Msa1170830 AT5G19160.2 34.505 313 186 8 20 317 30 338 1.45e-54 185
Msa1170830 AT3G11570.1 33.828 337 198 7 100 425 98 420 2.34e-54 186
Msa1170830 AT3G62390.2 38.113 265 143 8 68 317 117 375 9.30e-52 179
Msa1170830 AT3G06080.1 35.743 249 146 5 76 313 93 338 6.06e-50 172
Msa1170830 AT5G20590.2 37.450 251 144 5 72 313 130 376 1.88e-49 172
Msa1170830 AT2G30900.2 35.271 258 145 8 68 315 23 268 8.85e-47 162
Msa1170830 AT2G31110.3 35.088 228 135 5 83 308 45 261 1.98e-44 156
Msa1170830 AT1G70230.1 29.609 358 221 11 78 424 74 411 2.07e-43 157
Msa1170830 AT5G58600.2 33.473 239 140 5 83 315 66 291 1.16e-42 152
Msa1170830 AT2G34070.2 32.364 275 156 7 154 424 1 249 2.64e-42 150
Msa1170830 AT3G14850.1 34.066 273 150 6 154 421 1 248 4.96e-42 149
Msa1170830 AT3G28150.1 28.000 425 245 16 18 422 25 408 1.89e-41 152
Msa1170830 AT5G06230.3 34.524 252 134 7 83 313 62 303 9.25e-41 147
Msa1170830 AT5G15900.1 29.082 392 247 12 50 424 32 409 4.75e-40 148
Msa1170830 AT5G15900.2 29.082 392 247 12 50 424 51 428 5.81e-40 148
Msa1170830 AT5G15890.1 28.652 356 220 10 80 421 184 519 3.14e-38 145
Msa1170830 AT5G64020.1 29.301 372 213 16 83 424 57 408 1.11e-37 141
Msa1170830 AT5G64470.3 29.915 351 218 13 83 420 55 390 3.09e-37 140
Msa1170830 AT3G02440.1 26.966 356 232 11 83 424 127 468 7.38e-37 140
Msa1170830 AT3G02440.2 26.966 356 232 11 83 424 135 476 1.03e-36 140
Msa1170830 AT5G64470.2 29.494 356 219 12 83 420 55 396 2.05e-36 138
Msa1170830 AT1G01430.1 29.661 354 224 12 83 420 99 443 1.81e-35 136
Msa1170830 AT4G25360.1 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa1170830 AT4G25360.2 26.344 372 230 13 76 421 168 521 2.09e-35 137
Msa1170830 AT4G23790.1 25.059 427 276 17 21 424 22 427 8.86e-35 134
Msa1170830 AT5G51640.1 27.174 368 225 15 80 421 135 485 4.38e-34 133
Msa1170830 AT2G37720.1 28.571 385 218 17 66 424 129 482 5.56e-34 132
Msa1170830 AT4G01080.1 29.429 350 221 14 83 420 91 426 1.05e-33 131
Msa1170830 AT2G14530.1 26.519 362 229 10 83 424 62 406 1.17e-33 130
Msa1170830 AT2G31110.1 33.621 232 127 8 195 424 8 214 6.24e-31 118
Msa1170830 AT5G20680.6 26.178 382 208 16 79 424 114 457 1.06e-29 120
Msa1170830 AT5G20680.2 26.178 382 208 16 79 424 190 533 1.77e-29 120
Msa1170830 AT5G20680.4 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1170830 AT5G20680.1 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1170830 AT5G20680.3 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1170830 AT5G20680.5 26.178 382 208 16 79 424 208 551 1.82e-29 120
Msa1170830 AT4G11090.1 23.040 421 288 15 23 421 16 422 7.15e-27 112
Msa1170830 AT5G64470.1 27.985 268 178 9 83 340 55 317 3.72e-23 99.8
Msa1170830 AT5G64470.4 27.715 267 178 9 83 339 55 316 1.27e-22 98.2

Find 135 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATGGAGTTCCCTCTTT+TGG 0.157557 8_1:-182212 None:intergenic
TACCTGGTCTTCAAGATAAT+TGG 0.191574 8_1:+185115 Msa1170830:CDS
TTGGAAAGGTGTTGACATTT+TGG 0.194283 8_1:+184006 Msa1170830:CDS
GCTAATTGCTCTTTAGTTAA+AGG 0.201296 8_1:-185054 None:intergenic
ACTCCATTGTGATGTCTTAT+TGG 0.212538 8_1:+182224 Msa1170830:five_prime_UTR
ACACATTTGTTCAAATATAA+AGG 0.225938 8_1:+185181 Msa1170830:three_prime_UTR
TGATTAATAATTCCACATAC+TGG 0.251655 8_1:+184887 Msa1170830:CDS
TGTCGGGTCTCCCATGTTCT+TGG 0.253030 8_1:-183015 None:intergenic
ATGCAACAATTGAGTTCTAT+TGG 0.254941 8_1:+183885 Msa1170830:CDS
GAATTTGGTATTTCAGTTAT+AGG 0.257936 8_1:-185429 None:intergenic
TCAATAAGCATGGTAGAAAT+TGG 0.262180 8_1:+183987 Msa1170830:CDS
ATTGAATGTACTAGTATATT+AGG 0.272300 8_1:-182324 None:intergenic
TTGAAGTGTGTGCATCTTTA+CGG 0.287806 8_1:-185011 None:intergenic
TGAATTATCTATATATTGTT+AGG 0.290624 8_1:+185512 Msa1170830:three_prime_UTR
ATCAAAGGTTTCCATGGATT+TGG 0.299044 8_1:-183345 None:intergenic
ATCATATATTGTTGTTCTCT+TGG 0.308084 8_1:+185205 Msa1170830:three_prime_UTR
CTAATTGCTCTTTAGTTAAA+GGG 0.317364 8_1:-185053 None:intergenic
GAAGATTTCATGTGCATCTT+TGG 0.320970 8_1:+182566 Msa1170830:CDS
TATTCAATGGAAGGTGGGTT+TGG 0.326490 8_1:+182927 Msa1170830:CDS
TGAACTTATGTCTTTGTTGA+TGG 0.335672 8_1:-182276 None:intergenic
CTCCATTGTGATGTCTTATT+GGG 0.337032 8_1:+182225 Msa1170830:five_prime_UTR
GTGATGTTAAGAAATGTAAT+TGG 0.337450 8_1:-184973 None:intergenic
TGGTATTCAACACTTATCTT+TGG 0.343420 8_1:+184026 Msa1170830:CDS
TCTCTTGGATCATGGACTCT+TGG 0.348285 8_1:+185220 Msa1170830:three_prime_UTR
ACACAATCAGCATAGCTTAC+AGG 0.357812 8_1:-185081 None:intergenic
CACATTTGTTCAAATATAAA+GGG 0.368100 8_1:+185182 Msa1170830:three_prime_UTR
ATTCAATGGAAGGTGGGTTT+GGG 0.383497 8_1:+182928 Msa1170830:CDS
CGGACAATGCTGTTGTTCAT+AGG 0.387335 8_1:+183933 Msa1170830:CDS
CTCTTGGATCATGGACTCTT+GGG 0.388166 8_1:+185221 Msa1170830:three_prime_UTR
ATTGTTGTTCTCTTGGATCA+TGG 0.391390 8_1:+185212 Msa1170830:three_prime_UTR
ACGACCTTTGCTTCTTAGAA+TGG 0.393423 8_1:-182483 None:intergenic
CCAATGTTGGTAATCTTTGT+CGG 0.403476 8_1:-183032 None:intergenic
CTATGAAAAGTATGCTTAGA+TGG 0.410597 8_1:+184261 Msa1170830:CDS
TCTGTCTTCCCCACCGCAAA+TGG 0.411531 8_1:-182890 None:intergenic
TGCAACAATTGAGTTCTATT+GGG 0.419007 8_1:+183886 Msa1170830:CDS
GGTATTTCAGTTATAGGAAA+GGG 0.419698 8_1:-185423 None:intergenic
TATTCAACACTTATCTTTGG+TGG 0.424745 8_1:+184029 Msa1170830:CDS
TCCATGGATTTGGCATCCTC+AGG 0.427436 8_1:-183335 None:intergenic
AAATTAGTTATACGTATATG+AGG 0.427945 8_1:+182175 Msa1170830:five_prime_UTR
ACTAGTATATTAGGAGAAAG+TGG 0.429923 8_1:-182315 None:intergenic
ACTTATCTTTGGTGGATGAC+TGG 0.435500 8_1:+184037 Msa1170830:CDS
ATATGGCAGGAGTATAGATT+GGG 0.440954 8_1:+184825 Msa1170830:intron
TATATGGCAGGAGTATAGAT+TGG 0.441176 8_1:+184824 Msa1170830:intron
TAAATAATAGTGAGTTGAGT+TGG 0.441530 8_1:+182413 Msa1170830:five_prime_UTR
ATAGATTGGGGAGGTGAGCC+AGG 0.443479 8_1:+184838 Msa1170830:CDS
GTTAGTAGTTGTTCAAAGAA+AGG 0.448939 8_1:+185362 Msa1170830:three_prime_UTR
ATAGTGAGTTGAGTTGGATT+TGG 0.450733 8_1:+182419 Msa1170830:five_prime_UTR
CATCCTCAGGAATGAGTTGA+TGG 0.450737 8_1:-183322 None:intergenic
CAATGTTGGTAATCTTTGTC+GGG 0.457780 8_1:-183031 None:intergenic
CACTCCGATTCCTCGTACAA+TGG 0.458569 8_1:-182965 None:intergenic
GCTAGAAGGGTGAAAAGGTA+AGG 0.463485 8_1:-182512 None:intergenic
TGAAAGCTAGAAGGGTGAAA+AGG 0.463624 8_1:-182517 None:intergenic
ACGACCATTGTACGAGGAAT+CGG 0.464432 8_1:+182961 Msa1170830:CDS
GATTAATAATTCCACATACT+GGG 0.472858 8_1:+184888 Msa1170830:CDS
TGTGTACATTGGTGTTTACC+TGG 0.477328 8_1:+185099 Msa1170830:CDS
TGGAAGGTGGGTTTGGGACG+AGG 0.477341 8_1:+182934 Msa1170830:CDS
CACAATCAGCATAGCTTACA+GGG 0.477761 8_1:-185080 None:intergenic
AAAAGTATAATGCAAGTGAT+TGG 0.480921 8_1:+184925 Msa1170830:CDS
TCTGATAGAATTGTAAGAAA+AGG 0.484458 8_1:+183959 Msa1170830:CDS
TGATTAATCTTTGTTTGCAA+AGG 0.491547 8_1:+185286 Msa1170830:three_prime_UTR
TAGTGAATCAAAGGTTTCCA+TGG 0.492850 8_1:-183351 None:intergenic
GGAAAGAGGATGATGTTTGT+TGG 0.493089 8_1:+183254 Msa1170830:CDS
GCAGATCAAGGTGCCATTTG+CGG 0.494304 8_1:+182877 Msa1170830:CDS
GAAGGGTGTGATATATTCAA+TGG 0.494550 8_1:+182914 Msa1170830:CDS
GTGATATATTCAATGGAAGG+TGG 0.495098 8_1:+182921 Msa1170830:CDS
TCTTTGGTGGATGACTGGCT+TGG 0.496770 8_1:+184042 Msa1170830:CDS
TTGTGATGTCTTATTGGGTA+AGG 0.497193 8_1:+182230 Msa1170830:five_prime_UTR
TCATTGTAACAACTTCCTCC+TGG 0.501058 8_1:-184856 None:intergenic
GAGTTTGATTCTAGAAGAAA+AGG 0.501756 8_1:-183911 None:intergenic
TGTTCTTGGCAAGTCAATTG+AGG 0.502642 8_1:-183001 None:intergenic
AACTGAGGATGCTTATCGTA+TGG 0.502726 8_1:+184238 Msa1170830:CDS
CTAGTATATTAGGAGAAAGT+GGG 0.504465 8_1:-182314 None:intergenic
CAATTGACTTGCCAAGAACA+TGG 0.509598 8_1:+183004 Msa1170830:CDS
GTCAATTGAGGTTGTATGTA+CGG 0.512274 8_1:-182989 None:intergenic
GACAAATTCTTCTCCACACC+TGG 0.515054 8_1:+182617 Msa1170830:CDS
ACTAGCATGTCACCTTCTCA+TGG 0.524451 8_1:+184326 Msa1170830:CDS
ACCACTTAAGTGCCCAAAAG+AGG 0.525177 8_1:+182199 Msa1170830:five_prime_UTR
TTCCAATTATCTTGAAGACC+AGG 0.528264 8_1:-185117 None:intergenic
GAAAACAGTTAGTGAATCAA+AGG 0.528871 8_1:-183360 None:intergenic
TGATATATTCAATGGAAGGT+GGG 0.529302 8_1:+182922 Msa1170830:CDS
CTAGAAGGGTGAAAAGGTAA+GGG 0.530481 8_1:-182511 None:intergenic
AGAATGGAAGAAGGTGAGAA+TGG 0.531203 8_1:-182467 None:intergenic
GCGGTGGGGAAGACAGAGGA+AGG 0.532473 8_1:+182896 Msa1170830:CDS
ACAATCAGAACCCCAGTATG+TGG 0.541987 8_1:-184899 None:intergenic
TTGCTTCTTAGAATGGAAGA+AGG 0.542128 8_1:-182476 None:intergenic
CTTCAATCTATAAGAAGCAA+TGG 0.542329 8_1:+185028 Msa1170830:CDS
TGGTATTTCAGTTATAGGAA+AGG 0.542790 8_1:-185424 None:intergenic
TCCTGAGGATGCCAAATCCA+TGG 0.545590 8_1:+183334 Msa1170830:CDS
GTACTTTACTCTAAAAGACT+TGG 0.546625 8_1:-182368 None:intergenic
GAGGGTGAAGAAGCAGATCA+AGG 0.550519 8_1:+182865 Msa1170830:CDS
TATACTCACTTTCCATGAGA+AGG 0.556322 8_1:-184338 None:intergenic
ATCAAGGTGCCATTTGCGGT+GGG 0.557278 8_1:+182881 Msa1170830:CDS
GGTGTGATATATTCAATGGA+AGG 0.558509 8_1:+182918 Msa1170830:CDS
AAGGAAGAAAGAAATGAATG+AGG 0.563328 8_1:+185326 Msa1170830:three_prime_UTR
TAGAAGGGTGAAAAGGTAAG+GGG 0.565912 8_1:-182510 None:intergenic
GATCAAGGTGCCATTTGCGG+TGG 0.568008 8_1:+182880 Msa1170830:CDS
CCGACAAAGATTACCAACAT+TGG 0.570402 8_1:+183032 Msa1170830:CDS
AACAATGGACTCACCAGGTG+TGG 0.570501 8_1:-182630 None:intergenic
GTATTTCAGTTATAGGAAAG+GGG 0.570896 8_1:-185422 None:intergenic
GTGTGGAGAAGAATTTGTCT+TGG 0.571094 8_1:-182613 None:intergenic
TTGAAACACTACGTGGAAAG+AGG 0.579791 8_1:+183240 Msa1170830:CDS
CAGGAATGAGTTGATGGAGA+AGG 0.584159 8_1:-183316 None:intergenic
ATTAATAATTCCACATACTG+GGG 0.584467 8_1:+184889 Msa1170830:CDS
TATTTCAGTTATAGGAAAGG+GGG 0.584658 8_1:-185421 None:intergenic
CCACTTAAGTGCCCAAAAGA+GGG 0.592767 8_1:+182200 Msa1170830:five_prime_UTR
TCTTCCATTCTAAGAAGCAA+AGG 0.595126 8_1:+182479 Msa1170830:CDS
AAAGGTTCAATCAATAAGCA+TGG 0.595162 8_1:+183977 Msa1170830:CDS
ACAAAGATTACCAACATTGG+AGG 0.598469 8_1:+183035 Msa1170830:CDS
GGATACATGTGACGATAACA+AGG 0.600528 8_1:+185307 Msa1170830:three_prime_UTR
GTGACACTTTCATTTGTACA+CGG 0.608400 8_1:+185245 Msa1170830:three_prime_UTR
ATGGAAGAAGGTGAGAATGG+TGG 0.608866 8_1:-182464 None:intergenic
GTTGGAGACTCTCTCAATCG+TGG 0.612428 8_1:+183272 Msa1170830:CDS
GTGGTTGCCACCTCCAATGT+TGG 0.612456 8_1:-183045 None:intergenic
AATTGACTTGCCAAGAACAT+GGG 0.614611 8_1:+183005 Msa1170830:CDS
CACGGAAAAGAGTATAGCTA+CGG 0.614974 8_1:+185263 Msa1170830:three_prime_UTR
AAGATTACCAACATTGGAGG+TGG 0.615305 8_1:+183038 Msa1170830:CDS
TATGAAAAGTATGCTTAGAT+GGG 0.615716 8_1:+184262 Msa1170830:CDS
AATTAGTTATACGTATATGA+GGG 0.618030 8_1:+182176 Msa1170830:five_prime_UTR
TCAAGGTGCCATTTGCGGTG+GGG 0.620684 8_1:+182882 Msa1170830:CDS
TATGGCAGGAGTATAGATTG+GGG 0.621719 8_1:+184826 Msa1170830:intron
AAGCATGGTAGAAATTGGAA+AGG 0.640034 8_1:+183992 Msa1170830:CDS
CATTGGAGGTGGCAACCACA+CGG 0.642871 8_1:+183049 Msa1170830:CDS
TCTCCATCAACTCATTCCTG+AGG 0.645543 8_1:+183319 Msa1170830:CDS
AAAGTATGCTTAGATGGGTG+AGG 0.645663 8_1:+184267 Msa1170830:CDS
GATTGTCACAATTTCAACTG+AGG 0.649875 8_1:+184223 Msa1170830:CDS
GCTATGCTGATTGTGTACAT+TGG 0.671303 8_1:+185088 Msa1170830:CDS
GTATATTAGGAGAAAGTGGG+AGG 0.692881 8_1:-182311 None:intergenic
GGCAGGAGTATAGATTGGGG+AGG 0.697427 8_1:+184829 Msa1170830:intron
TTAGGAAGATCGCAACCGTG+TGG 0.699752 8_1:-183064 None:intergenic
CGGTGGGGAAGACAGAGGAA+GGG 0.705520 8_1:+182897 Msa1170830:CDS
GATTGGGGAGGTGAGCCAGG+AGG 0.708567 8_1:+184841 Msa1170830:CDS
ATTTGCGGTGGGGAAGACAG+AGG 0.711107 8_1:+182892 Msa1170830:CDS
GGATACACGACCATTGTACG+AGG 0.724636 8_1:+182955 Msa1170830:CDS
TACCCAATAAGACATCACAA+TGG 0.732110 8_1:-182227 None:intergenic
TTGATGCTTGAAACACTACG+TGG 0.805901 8_1:+183233 Msa1170830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAATAATTATATTTAG+TGG - chr8_1:183761-183780 None:intergenic 10.0%
!!! TAATTTTCAAATAATCAAAA+TGG - chr8_1:184139-184158 None:intergenic 10.0%
!! TATTATAGATCAATTTAAAT+AGG - chr8_1:184449-184468 None:intergenic 10.0%
!! ATTTAAATTGATCTATAATA+TGG + chr8_1:184449-184468 Msa1170830:intron 10.0%
!! TTAAAATCTTATGAATATGT+CGG - chr8_1:182757-182776 None:intergenic 15.0%
!!! AATATATTATGCCTTTTTTA+TGG + chr8_1:182800-182819 Msa1170830:intron 15.0%
!!! TTTATTTGATGTTTTGTATT+TGG + chr8_1:182823-182842 Msa1170830:intron 15.0%
!!! TTATTTGATGTTTTGTATTT+GGG + chr8_1:182824-182843 Msa1170830:intron 15.0%
!! TTCACTTTAATGTAATAATT+TGG + chr8_1:183624-183643 Msa1170830:intron 15.0%
!! TGAAAATCAACAATAAAAAA+TGG - chr8_1:183845-183864 None:intergenic 15.0%
!! AAGTCTAAAAAAATTGAAAA+GGG - chr8_1:184177-184196 None:intergenic 15.0%
!!! AATTGTAGAGATTTTTTTTA+AGG - chr8_1:184555-184574 None:intergenic 15.0%
!!! AAAAAAAATCTCTACAATTT+TGG + chr8_1:184556-184575 Msa1170830:intron 15.0%
!! TAAACAAAACTTTATTCTTT+AGG + chr8_1:184642-184661 Msa1170830:intron 15.0%
!! CTAATATTTCAACAAATATA+TGG + chr8_1:184808-184827 Msa1170830:intron 15.0%
!! TGAATTATCTATATATTGTT+AGG + chr8_1:185512-185531 Msa1170830:three_prime_UTR 15.0%
!!! TTTTAACTATTAGTGTTTTT+TGG - chr8_1:185554-185573 None:intergenic 15.0%
!! AAATTAGTTATACGTATATG+AGG + chr8_1:182175-182194 Msa1170830:five_prime_UTR 20.0%
!! AATTAGTTATACGTATATGA+GGG + chr8_1:182176-182195 Msa1170830:five_prime_UTR 20.0%
!! ATTGAATGTACTAGTATATT+AGG - chr8_1:182327-182346 None:intergenic 20.0%
!!! TTTTACATCAAAAAGTATAG+AGG + chr8_1:182690-182709 Msa1170830:intron 20.0%
!! TAAAATCTTATGAATATGTC+GGG - chr8_1:182756-182775 None:intergenic 20.0%
!! CATCAAATAAACCATAAAAA+AGG - chr8_1:182814-182833 None:intergenic 20.0%
!!! TATTTGATGTTTTGTATTTG+GGG + chr8_1:182825-182844 Msa1170830:intron 20.0%
!! GCATAAATAAATACTTACTT+AGG - chr8_1:183085-183104 None:intergenic 20.0%
!! TTCATAACAATAATGAAGAT+CGG - chr8_1:183412-183431 None:intergenic 20.0%
!! TTCATTATTGTTATGAATGA+TGG + chr8_1:183415-183434 Msa1170830:intron 20.0%
!! AGTTAATTATAAGAGTGAAT+CGG + chr8_1:183560-183579 Msa1170830:intron 20.0%
!! CAAATAATCAAAATGGAAAA+GGG - chr8_1:184132-184151 None:intergenic 20.0%
!! TCAAATAATCAAAATGGAAA+AGG - chr8_1:184133-184152 None:intergenic 20.0%
!! CAAGTCTAAAAAAATTGAAA+AGG - chr8_1:184178-184197 None:intergenic 20.0%
!!! CTTTTCAATTTTTTTAGACT+TGG + chr8_1:184176-184195 Msa1170830:intron 20.0%
!! ACACATTTGTTCAAATATAA+AGG + chr8_1:185181-185200 Msa1170830:three_prime_UTR 20.0%
!! CACATTTGTTCAAATATAAA+GGG + chr8_1:185182-185201 Msa1170830:three_prime_UTR 20.0%
!!! ACTACAAAAAATTTGGAATT+TGG - chr8_1:185447-185466 None:intergenic 20.0%
!!! TCCAAATTTTTTGTAGTTTT+TGG + chr8_1:185450-185469 Msa1170830:three_prime_UTR 20.0%
!! ACCAAAAACTACAAAAAATT+TGG - chr8_1:185454-185473 None:intergenic 20.0%
!! TAAATAATAGTGAGTTGAGT+TGG + chr8_1:182413-182432 Msa1170830:five_prime_UTR 25.0%
! AAAAAGAATGAAAGCTAGAA+GGG - chr8_1:182528-182547 None:intergenic 25.0%
!! CTTTTTGTTACTATTCTCTA+TGG + chr8_1:182542-182561 Msa1170830:CDS 25.0%
!!! TGTAGCATTTTTTGAAACAA+TGG - chr8_1:182648-182667 None:intergenic 25.0%
! AAAATCTTATGAATATGTCG+GGG - chr8_1:182755-182774 None:intergenic 25.0%
!! ATTTGATGTTTTGTATTTGG+GGG + chr8_1:182826-182845 Msa1170830:intron 25.0%
! TTGATAAAAGAACGAGATAA+TGG + chr8_1:183527-183546 Msa1170830:intron 25.0%
!! TGATTTTCATGTGTTTAATC+AGG + chr8_1:183856-183875 Msa1170830:intron 25.0%
!!! TTTTCTTCTAGAATCAAACT+CGG + chr8_1:183913-183932 Msa1170830:CDS 25.0%
! TCTGATAGAATTGTAAGAAA+AGG + chr8_1:183959-183978 Msa1170830:CDS 25.0%
!!! ATCTTTTGATGATGAAGTAA+AGG + chr8_1:184199-184218 Msa1170830:CDS 25.0%
!! TATGAAAAGTATGCTTAGAT+GGG + chr8_1:184262-184281 Msa1170830:CDS 25.0%
!!! GTTTTGTTTATAATAGTGAC+CGG - chr8_1:184632-184651 None:intergenic 25.0%
!! ATACGCAAAATTTCACATTT+TGG - chr8_1:184731-184750 None:intergenic 25.0%
! GAAATATTAGAACTTAGAAG+TGG - chr8_1:184798-184817 None:intergenic 25.0%
! TATTTCAACAAATATATGGC+AGG + chr8_1:184812-184831 Msa1170830:intron 25.0%
! TGATTAATAATTCCACATAC+TGG + chr8_1:184887-184906 Msa1170830:CDS 25.0%
! GATTAATAATTCCACATACT+GGG + chr8_1:184888-184907 Msa1170830:CDS 25.0%
! ATTAATAATTCCACATACTG+GGG + chr8_1:184889-184908 Msa1170830:CDS 25.0%
!! AAAAGTATAATGCAAGTGAT+TGG + chr8_1:184925-184944 Msa1170830:CDS 25.0%
! GTGATGTTAAGAAATGTAAT+TGG - chr8_1:184976-184995 None:intergenic 25.0%
! CTAATTGCTCTTTAGTTAAA+GGG - chr8_1:185056-185075 None:intergenic 25.0%
!! ACAAATGTGTACAATTTTCA+AGG - chr8_1:185171-185190 None:intergenic 25.0%
!! ATCATATATTGTTGTTCTCT+TGG + chr8_1:185205-185224 Msa1170830:three_prime_UTR 25.0%
!! TGATTAATCTTTGTTTGCAA+AGG + chr8_1:185286-185305 Msa1170830:three_prime_UTR 25.0%
! GAATTTGGTATTTCAGTTAT+AGG - chr8_1:185432-185451 None:intergenic 25.0%
TGAACTTATGTCTTTGTTGA+TGG - chr8_1:182279-182298 None:intergenic 30.0%
CTAGTATATTAGGAGAAAGT+GGG - chr8_1:182317-182336 None:intergenic 30.0%
ACTAGTATATTAGGAGAAAG+TGG - chr8_1:182318-182337 None:intergenic 30.0%
! GTACTTTACTCTAAAAGACT+TGG - chr8_1:182371-182390 None:intergenic 30.0%
CAAAAAGAATGAAAGCTAGA+AGG - chr8_1:182529-182548 None:intergenic 30.0%
! TTTGATGTTTTGTATTTGGG+GGG + chr8_1:182827-182846 Msa1170830:intron 30.0%
TGATATATTCAATGGAAGGT+GGG + chr8_1:182922-182941 Msa1170830:CDS 30.0%
GAAAACAGTTAGTGAATCAA+AGG - chr8_1:183363-183382 None:intergenic 30.0%
AGAAATCATAAGTATTCGTG+TGG + chr8_1:183474-183493 Msa1170830:intron 30.0%
GAAATCATAAGTATTCGTGT+GGG + chr8_1:183475-183494 Msa1170830:intron 30.0%
AATCAACTAGAGTTAGTTGT+TGG - chr8_1:183606-183625 None:intergenic 30.0%
ATGCAACAATTGAGTTCTAT+TGG + chr8_1:183885-183904 Msa1170830:CDS 30.0%
TGCAACAATTGAGTTCTATT+GGG + chr8_1:183886-183905 Msa1170830:CDS 30.0%
! GAGTTTGATTCTAGAAGAAA+AGG - chr8_1:183914-183933 None:intergenic 30.0%
AAAGGTTCAATCAATAAGCA+TGG + chr8_1:183977-183996 Msa1170830:CDS 30.0%
TCAATAAGCATGGTAGAAAT+TGG + chr8_1:183987-184006 Msa1170830:CDS 30.0%
TGGTATTCAACACTTATCTT+TGG + chr8_1:184026-184045 Msa1170830:CDS 30.0%
TATTCAACACTTATCTTTGG+TGG + chr8_1:184029-184048 Msa1170830:CDS 30.0%
GCTTGTTTGAATAGAAAAGA+TGG - chr8_1:184088-184107 None:intergenic 30.0%
! CTATGAAAAGTATGCTTAGA+TGG + chr8_1:184261-184280 Msa1170830:CDS 30.0%
!!! AAGAAGACTCTAGTTTTCTT+TGG - chr8_1:184305-184324 None:intergenic 30.0%
! GAAACCAAACCATACAATTT+TGG + chr8_1:184526-184545 Msa1170830:intron 30.0%
! TTAAGGTGTCCAAAATTGTA+TGG - chr8_1:184538-184557 None:intergenic 30.0%
TGCGTATAATAATGATCAGA+AGG + chr8_1:184744-184763 Msa1170830:intron 30.0%
CTTCAATCTATAAGAAGCAA+TGG + chr8_1:185028-185047 Msa1170830:CDS 30.0%
GCTAATTGCTCTTTAGTTAA+AGG - chr8_1:185057-185076 None:intergenic 30.0%
AAGGAAGAAAGAAATGAATG+AGG + chr8_1:185326-185345 Msa1170830:three_prime_UTR 30.0%
GTTAGTAGTTGTTCAAAGAA+AGG + chr8_1:185362-185381 Msa1170830:three_prime_UTR 30.0%
TATTTCAGTTATAGGAAAGG+GGG - chr8_1:185424-185443 None:intergenic 30.0%
GTATTTCAGTTATAGGAAAG+GGG - chr8_1:185425-185444 None:intergenic 30.0%
GGTATTTCAGTTATAGGAAA+GGG - chr8_1:185426-185445 None:intergenic 30.0%
TGGTATTTCAGTTATAGGAA+AGG - chr8_1:185427-185446 None:intergenic 30.0%
ACTCCATTGTGATGTCTTAT+TGG + chr8_1:182224-182243 Msa1170830:five_prime_UTR 35.0%
! CTCCATTGTGATGTCTTATT+GGG + chr8_1:182225-182244 Msa1170830:five_prime_UTR 35.0%
TACCCAATAAGACATCACAA+TGG - chr8_1:182230-182249 None:intergenic 35.0%
! TTGTGATGTCTTATTGGGTA+AGG + chr8_1:182230-182249 Msa1170830:five_prime_UTR 35.0%
!! ATAGTGAGTTGAGTTGGATT+TGG + chr8_1:182419-182438 Msa1170830:five_prime_UTR 35.0%
TTGCTTCTTAGAATGGAAGA+AGG - chr8_1:182479-182498 None:intergenic 35.0%
! TCTTCCATTCTAAGAAGCAA+AGG + chr8_1:182479-182498 Msa1170830:CDS 35.0%
GAAGATTTCATGTGCATCTT+TGG + chr8_1:182566-182585 Msa1170830:CDS 35.0%
GGGTGAATAATGAAAAGAGA+GGG + chr8_1:182847-182866 Msa1170830:intron 35.0%
! GAAGGGTGTGATATATTCAA+TGG + chr8_1:182914-182933 Msa1170830:CDS 35.0%
GGTGTGATATATTCAATGGA+AGG + chr8_1:182918-182937 Msa1170830:CDS 35.0%
GTGATATATTCAATGGAAGG+TGG + chr8_1:182921-182940 Msa1170830:CDS 35.0%
GTCAATTGAGGTTGTATGTA+CGG - chr8_1:182992-183011 None:intergenic 35.0%
!! AATTGACTTGCCAAGAACAT+GGG + chr8_1:183005-183024 Msa1170830:CDS 35.0%
!! CAATGTTGGTAATCTTTGTC+GGG - chr8_1:183034-183053 None:intergenic 35.0%
!! CCAATGTTGGTAATCTTTGT+CGG - chr8_1:183035-183054 None:intergenic 35.0%
ACAAAGATTACCAACATTGG+AGG + chr8_1:183035-183054 Msa1170830:CDS 35.0%
ATCAAAGGTTTCCATGGATT+TGG - chr8_1:183348-183367 None:intergenic 35.0%
TAGTGAATCAAAGGTTTCCA+TGG - chr8_1:183354-183373 None:intergenic 35.0%
! ACTAACTGTTTTCACAGCAA+AGG + chr8_1:183370-183389 Msa1170830:CDS 35.0%
! ACTAGAGTTAGTTGTTGGTT+AGG - chr8_1:183601-183620 None:intergenic 35.0%
AAGCATGGTAGAAATTGGAA+AGG + chr8_1:183992-184011 Msa1170830:CDS 35.0%
!!! TTGGAAAGGTGTTGACATTT+TGG + chr8_1:184006-184025 Msa1170830:CDS 35.0%
GATTGTCACAATTTCAACTG+AGG + chr8_1:184223-184242 Msa1170830:CDS 35.0%
TATACTCACTTTCCATGAGA+AGG - chr8_1:184341-184360 None:intergenic 35.0%
GTGTCCAAAATTGTATGGTT+TGG - chr8_1:184533-184552 None:intergenic 35.0%
! ACATGCTCGTTGTTAATTTG+AGG - chr8_1:184602-184621 None:intergenic 35.0%
TGTTTATAATAGTGACCGGA+GGG - chr8_1:184628-184647 None:intergenic 35.0%
TTGTTTATAATAGTGACCGG+AGG - chr8_1:184629-184648 None:intergenic 35.0%
! TATATGGCAGGAGTATAGAT+TGG + chr8_1:184824-184843 Msa1170830:intron 35.0%
! ATATGGCAGGAGTATAGATT+GGG + chr8_1:184825-184844 Msa1170830:intron 35.0%
TTGAAGTGTGTGCATCTTTA+CGG - chr8_1:185014-185033 None:intergenic 35.0%
! TACCTGGTCTTCAAGATAAT+TGG + chr8_1:185115-185134 Msa1170830:CDS 35.0%
TTCCAATTATCTTGAAGACC+AGG - chr8_1:185120-185139 None:intergenic 35.0%
! ATTGTTGTTCTCTTGGATCA+TGG + chr8_1:185212-185231 Msa1170830:three_prime_UTR 35.0%
GTGACACTTTCATTTGTACA+CGG + chr8_1:185245-185264 Msa1170830:three_prime_UTR 35.0%
! ACAATGGAGTTCCCTCTTTT+GGG - chr8_1:182214-182233 None:intergenic 40.0%
GTATATTAGGAGAAAGTGGG+AGG - chr8_1:182314-182333 None:intergenic 40.0%
AGAATGGAAGAAGGTGAGAA+TGG - chr8_1:182470-182489 None:intergenic 40.0%
ACGACCTTTGCTTCTTAGAA+TGG - chr8_1:182486-182505 None:intergenic 40.0%
TAGAAGGGTGAAAAGGTAAG+GGG - chr8_1:182513-182532 None:intergenic 40.0%
CTAGAAGGGTGAAAAGGTAA+GGG - chr8_1:182514-182533 None:intergenic 40.0%
TGAAAGCTAGAAGGGTGAAA+AGG - chr8_1:182520-182539 None:intergenic 40.0%
GTGTGGAGAAGAATTTGTCT+TGG - chr8_1:182616-182635 None:intergenic 40.0%
! TTTGAAACAATGGACTCACC+AGG - chr8_1:182638-182657 None:intergenic 40.0%
GCGAGTTTGAACTCTCTTAT+GGG - chr8_1:182731-182750 None:intergenic 40.0%
GGGGTGAATAATGAAAAGAG+AGG + chr8_1:182846-182865 Msa1170830:intron 40.0%
! TATTCAATGGAAGGTGGGTT+TGG + chr8_1:182927-182946 Msa1170830:CDS 40.0%
! ATTCAATGGAAGGTGGGTTT+GGG + chr8_1:182928-182947 Msa1170830:CDS 40.0%
TGTTCTTGGCAAGTCAATTG+AGG - chr8_1:183004-183023 None:intergenic 40.0%
!! CAATTGACTTGCCAAGAACA+TGG + chr8_1:183004-183023 Msa1170830:CDS 40.0%
CCGACAAAGATTACCAACAT+TGG + chr8_1:183032-183051 Msa1170830:CDS 40.0%
AAGATTACCAACATTGGAGG+TGG + chr8_1:183038-183057 Msa1170830:CDS 40.0%
TTGATGCTTGAAACACTACG+TGG + chr8_1:183233-183252 Msa1170830:CDS 40.0%
TTGAAACACTACGTGGAAAG+AGG + chr8_1:183240-183259 Msa1170830:CDS 40.0%
GGAAAGAGGATGATGTTTGT+TGG + chr8_1:183254-183273 Msa1170830:CDS 40.0%
TAGAGATCATAGTGATCAGC+AGG + chr8_1:183449-183468 Msa1170830:intron 40.0%
! ACTTATCTTTGGTGGATGAC+TGG + chr8_1:184037-184056 Msa1170830:CDS 40.0%
AACTGAGGATGCTTATCGTA+TGG + chr8_1:184238-184257 Msa1170830:CDS 40.0%
AAAGTATGCTTAGATGGGTG+AGG + chr8_1:184267-184286 Msa1170830:CDS 40.0%
! TATGGCAGGAGTATAGATTG+GGG + chr8_1:184826-184845 Msa1170830:intron 40.0%
TCATTGTAACAACTTCCTCC+TGG - chr8_1:184859-184878 None:intergenic 40.0%
CACAATCAGCATAGCTTACA+GGG - chr8_1:185083-185102 None:intergenic 40.0%
ACACAATCAGCATAGCTTAC+AGG - chr8_1:185084-185103 None:intergenic 40.0%
! GCTATGCTGATTGTGTACAT+TGG + chr8_1:185088-185107 Msa1170830:CDS 40.0%
! TGTGTACATTGGTGTTTACC+TGG + chr8_1:185099-185118 Msa1170830:CDS 40.0%
! CACGGAAAAGAGTATAGCTA+CGG + chr8_1:185263-185282 Msa1170830:three_prime_UTR 40.0%
GGATACATGTGACGATAACA+AGG + chr8_1:185307-185326 Msa1170830:three_prime_UTR 40.0%
ACCACTTAAGTGCCCAAAAG+AGG + chr8_1:182199-182218 Msa1170830:five_prime_UTR 45.0%
CCACTTAAGTGCCCAAAAGA+GGG + chr8_1:182200-182219 Msa1170830:five_prime_UTR 45.0%
! CACAATGGAGTTCCCTCTTT+TGG - chr8_1:182215-182234 None:intergenic 45.0%
ATGGAAGAAGGTGAGAATGG+TGG - chr8_1:182467-182486 None:intergenic 45.0%
GCTAGAAGGGTGAAAAGGTA+AGG - chr8_1:182515-182534 None:intergenic 45.0%
GACAAATTCTTCTCCACACC+TGG + chr8_1:182617-182636 Msa1170830:CDS 45.0%
CGCGAGTTTGAACTCTCTTA+TGG - chr8_1:182732-182751 None:intergenic 45.0%
ACGACCATTGTACGAGGAAT+CGG + chr8_1:182961-182980 Msa1170830:CDS 45.0%
!! CAGGAATGAGTTGATGGAGA+AGG - chr8_1:183319-183338 None:intergenic 45.0%
TCTCCATCAACTCATTCCTG+AGG + chr8_1:183319-183338 Msa1170830:CDS 45.0%
! CATCCTCAGGAATGAGTTGA+TGG - chr8_1:183325-183344 None:intergenic 45.0%
CGGACAATGCTGTTGTTCAT+AGG + chr8_1:183933-183952 Msa1170830:CDS 45.0%
ACTAGCATGTCACCTTCTCA+TGG + chr8_1:184326-184345 Msa1170830:CDS 45.0%
ACAATCAGAACCCCAGTATG+TGG - chr8_1:184902-184921 None:intergenic 45.0%
TCTCTTGGATCATGGACTCT+TGG + chr8_1:185220-185239 Msa1170830:three_prime_UTR 45.0%
CTCTTGGATCATGGACTCTT+GGG + chr8_1:185221-185240 Msa1170830:three_prime_UTR 45.0%
! CCCTCTTTTGGGCACTTAAG+TGG - chr8_1:182203-182222 None:intergenic 50.0%
! AACAATGGACTCACCAGGTG+TGG - chr8_1:182633-182652 None:intergenic 50.0%
GAGGGTGAAGAAGCAGATCA+AGG + chr8_1:182865-182884 Msa1170830:CDS 50.0%
!! GCAGATCAAGGTGCCATTTG+CGG + chr8_1:182877-182896 Msa1170830:CDS 50.0%
!! ATCAAGGTGCCATTTGCGGT+GGG + chr8_1:182881-182900 Msa1170830:CDS 50.0%
GGATACACGACCATTGTACG+AGG + chr8_1:182955-182974 Msa1170830:CDS 50.0%
CACTCCGATTCCTCGTACAA+TGG - chr8_1:182968-182987 None:intergenic 50.0%
TTAGGAAGATCGCAACCGTG+TGG - chr8_1:183067-183086 None:intergenic 50.0%
GTTGGAGACTCTCTCAATCG+TGG + chr8_1:183272-183291 Msa1170830:CDS 50.0%
TCCTGAGGATGCCAAATCCA+TGG + chr8_1:183334-183353 Msa1170830:CDS 50.0%
TCCATGGATTTGGCATCCTC+AGG - chr8_1:183338-183357 None:intergenic 50.0%
GTTAACACGCGGAGGATACT+TGG - chr8_1:183656-183675 None:intergenic 50.0%
CGAGCAACAACGTTAACACG+CGG - chr8_1:183667-183686 None:intergenic 50.0%
! TCTTTGGTGGATGACTGGCT+TGG + chr8_1:184042-184061 Msa1170830:CDS 50.0%
!! GATCAAGGTGCCATTTGCGG+TGG + chr8_1:182880-182899 Msa1170830:CDS 55.0%
!! TCAAGGTGCCATTTGCGGTG+GGG + chr8_1:182882-182901 Msa1170830:CDS 55.0%
TCTGTCTTCCCCACCGCAAA+TGG - chr8_1:182893-182912 None:intergenic 55.0%
ATTTGCGGTGGGGAAGACAG+AGG + chr8_1:182892-182911 Msa1170830:CDS 55.0%
TGTCGGGTCTCCCATGTTCT+TGG - chr8_1:183018-183037 None:intergenic 55.0%
GTGGTTGCCACCTCCAATGT+TGG - chr8_1:183048-183067 None:intergenic 55.0%
CATTGGAGGTGGCAACCACA+CGG + chr8_1:183049-183068 Msa1170830:CDS 55.0%
GCAACAACGTTAACACGCGG+AGG - chr8_1:183664-183683 None:intergenic 55.0%
CAACGAGCATGTACACCCTC+CGG + chr8_1:184610-184629 Msa1170830:intron 55.0%
! GGCAGGAGTATAGATTGGGG+AGG + chr8_1:184829-184848 Msa1170830:intron 55.0%
ATAGATTGGGGAGGTGAGCC+AGG + chr8_1:184838-184857 Msa1170830:CDS 55.0%
CGGTGGGGAAGACAGAGGAA+GGG + chr8_1:182897-182916 Msa1170830:CDS 60.0%
! TGGAAGGTGGGTTTGGGACG+AGG + chr8_1:182934-182953 Msa1170830:CDS 60.0%
GCGGTGGGGAAGACAGAGGA+AGG + chr8_1:182896-182915 Msa1170830:CDS 65.0%
GATTGGGGAGGTGAGCCAGG+AGG + chr8_1:184841-184860 Msa1170830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 182160 185578 182160 ID=Msa1170830;Name=Msa1170830
chr8_1 mRNA 182160 185578 182160 ID=Msa1170830-mRNA-1;Parent=Msa1170830;Name=Msa1170830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=289|1|1|1|1|1|6|405|427
chr8_1 exon 182160 182638 182160 ID=Msa1170830-mRNA-1:exon:0;Parent=Msa1170830-mRNA-1
chr8_1 exon 182864 183086 182864 ID=Msa1170830-mRNA-1:exon:1;Parent=Msa1170830-mRNA-1
chr8_1 exon 183220 183391 183220 ID=Msa1170830-mRNA-1:exon:2;Parent=Msa1170830-mRNA-1
chr8_1 exon 183878 184077 183878 ID=Msa1170830-mRNA-1:exon:3;Parent=Msa1170830-mRNA-1
chr8_1 exon 184193 184351 184193 ID=Msa1170830-mRNA-1:exon:4;Parent=Msa1170830-mRNA-1
chr8_1 exon 184834 185578 184834 ID=Msa1170830-mRNA-1:exon:5;Parent=Msa1170830-mRNA-1
chr8_1 five_prime_UTR 182160 182448 182160 ID=Msa1170830-mRNA-1:five_prime_utr;Parent=Msa1170830-mRNA-1
chr8_1 CDS 182449 182638 182449 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 CDS 182864 183086 182864 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 CDS 183220 183391 183220 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 CDS 183878 184077 183878 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 CDS 184193 184351 184193 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 CDS 184834 185173 184834 ID=Msa1170830-mRNA-1:cds;Parent=Msa1170830-mRNA-1
chr8_1 three_prime_UTR 185174 185578 185174 ID=Msa1170830-mRNA-1:three_prime_utr;Parent=Msa1170830-mRNA-1
Gene Sequence

>Msa1170830

ATGCAAATAATGAAGCCACCATTCTCACCTTCTTCCATTCTAAGAAGCAAAGGTCGTTTCTCCCCTTACCTTTTCACCCTTCTAGCTTTCATTCTTTTTGTTACTATTCTCTATGGTGAAGATTTCATGTGCATCTTTGGTGAACAACTCTATAACTACTCTAACCAAGACAAATTCTTCTCCACACCTGAGAGGGTGAAGAAGCAGATCAAGGTGCCATTTGCGGTGGGGAAGACAGAGGAAGGGTGTGATATATTCAATGGAAGGTGGGTTTGGGACGAGGATACACGACCATTGTACGAGGAATCGGAGTGTCCGTACATACAACCTCAATTGACTTGCCAAGAACATGGGAGACCCGACAAAGATTACCAACATTGGAGGTGGCAACCACACGGTTGCGATCTTCCTAAATTCAATGCTAGCTTGATGCTTGAAACACTACGTGGAAAGAGGATGATGTTTGTTGGAGACTCTCTCAATCGTGGTCAATATGTTTCTTTTGTATGCCTTCTCCATCAACTCATTCCTGAGGATGCCAAATCCATGGAAACCTTTGATTCACTAACTGTTTTCACAGCAAAGGAATACAATGCAACAATTGAGTTCTATTGGGCACCTTTTCTTCTAGAATCAAACTCGGACAATGCTGTTGTTCATAGGATATCTGATAGAATTGTAAGAAAAGGTTCAATCAATAAGCATGGTAGAAATTGGAAAGGTGTTGACATTTTGGTATTCAACACTTATCTTTGGTGGATGACTGGCTTGGAGATGAAAATCTTACTTGGATCTTTTGATGATGAAGTAAAGGAGATTGTCACAATTTCAACTGAGGATGCTTATCGTATGGCTATGAAAAGTATGCTTAGATGGGTGAGGTTGAACATGAATCCAAAGAAAACTAGAGTCTTCTTCACTAGCATGTCACCTTCTCATGGAAAGAGTATAGATTGGGGAGGTGAGCCAGGAGGAAGTTGTTACAATGAAACAACTTTGATTAATAATTCCACATACTGGGGTTCTGATTGTAGAAAAAGTATAATGCAAGTGATTGGTGAAGTGTTAAGCAAAACAAAAGTTCCAATTACATTTCTTAACATCACACAACTCTCAAGTTACCGTAAAGATGCACACACTTCAATCTATAAGAAGCAATGGAGCCCTTTAACTAAAGAGCAATTAGCTAACCCTGTAAGCTATGCTGATTGTGTACATTGGTGTTTACCTGGTCTTCAAGATAATTGGAATGAACTTTTGTTTGCTAAGTTGTTTTATCCTTGA
Protein sequence

>Msa1170830

MQIMKPPFSPSSILRSKGRFSPYLFTLLAFILFVTILYGEDFMCIFGEQLYNYSNQDKFFSTPERVKKQIKVPFAVGKTEEGCDIFNGRWVWDEDTRPLYEESECPYIQPQLTCQEHGRPDKDYQHWRWQPHGCDLPKFNASLMLETLRGKRMMFVGDSLNRGQYVSFVCLLHQLIPEDAKSMETFDSLTVFTAKEYNATIEFYWAPFLLESNSDNAVVHRISDRIVRKGSINKHGRNWKGVDILVFNTYLWWMTGLEMKILLGSFDDEVKEIVTISTEDAYRMAMKSMLRWVRLNMNPKKTRVFFTSMSPSHGKSIDWGGEPGGSCYNETTLINNSTYWGSDCRKSIMQVIGEVLSKTKVPITFLNITQLSSYRKDAHTSIYKKQWSPLTKEQLANPVSYADCVHWCLPGLQDNWNELLFAKLFYP*