AlfalfaGEDB | Msa0761300

M. sativa cultivar zhongmu-4 / Msa0761300

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0761300	G7L3K3	87.555	458	38	5	1	457	1	440	0.0	690

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0079380	Msa0761300	0.804299	2.309510e-49	-8.615850e-47
Msa0084380	Msa0761300	0.806846	6.746629e-50	-8.615850e-47
Msa0264150	Msa0761300	0.808610	2.846052e-50	-8.615850e-47
Msa0358890	Msa0761300	0.802442	5.599824e-49	-8.615850e-47
Msa0504210	Msa0761300	0.819994	8.671028e-53	-8.615850e-47
Msa0720730	Msa0761300	0.854061	1.581615e-61	-8.615850e-47
Msa0761300	Msa0841850	0.858611	7.316630e-63	-8.615850e-47
Msa0761300	Msa0902510	0.807130	5.873723e-50	-8.615850e-47
Msa0761300	Msa0974070	0.801016	1.098325e-48	-8.615850e-47
Msa0761300	Msa1123380	0.821878	3.195156e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0761300	MtrunA17_Chr7g0247241	87.555	458	38	5	1	457	1	440	0.0	690
Msa0761300	MtrunA17_Chr5g0411521	82.213	461	48	6	1	457	46	476	0.0	675

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0761300	AT3G53440.2	40.136	294	139	7	160	446	249	512	4.54e-47	170
Msa0761300	AT3G53440.1	40.136	294	139	7	160	446	249	512	4.54e-47	170

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Find 112 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAAGAAAAGTAGCG+GGG	0.557669	5_2:+18480200	Msa0761300:CDS
AAAAGGAAACACGATGGTGG+TGG	0.607985	5_2:+18480324	Msa0761300:CDS
AAAAGGAAGAGAAAGCGCGG+TGG	0.749477	5_2:+18480393	Msa0761300:CDS
AAAAGGAGAAGAAAGCACGG+TGG	0.843786	5_2:+18480246	Msa0761300:CDS
AAAATCTGTTGCAGGTACCT+GGG	0.591061	5_2:+18482455	Msa0761300:intron
AAAGAGAAGAAAAGTAGCGG+GGG	0.585434	5_2:+18480201	Msa0761300:CDS
AAAGTTGATCAAAATCATAA+AGG	0.382393	5_2:+18482709	Msa0761300:CDS
AAATATACAAAGATCACAGT+AGG	0.592908	5_2:+18483521	Msa0761300:three_prime_UTR
AAGAAAGAAGAGATTGAAGG+TGG	0.529397	5_2:+18480291	Msa0761300:CDS
AAGATGCTGCTCTTCTCCTA+AGG	0.500545	5_2:-18483005	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGTTAAAGTGAGTGAATT+TGG	+	chr5_2:18480141-18480160	Msa0761300:CDS	30.0%
TTGAATTATGTGTCTAAACG+AGG	+	chr5_2:18480623-18480642	Msa0761300:intron	30.0%
ACTAAATACTGTCTACTACA+TGG	-	chr5_2:18480725-18480744	None:intergenic	30.0%
AGACAGTATTTAGTGTGATA+AGG	+	chr5_2:18480731-18480750	Msa0761300:intron	30.0%
GTATTTAGTGTGATAAGGTT+TGG	+	chr5_2:18480736-18480755	Msa0761300:intron	30.0%
CAACACTATTAGTAAATAGC+TGG	+	chr5_2:18480848-18480867	Msa0761300:intron	30.0%
AATGCCTTCTATAAAACCAT+TGG	-	chr5_2:18481265-18481284	None:intergenic	30.0%
GAAGTTTCAAATCTCACAAT+TGG	-	chr5_2:18481761-18481780	None:intergenic	30.0%
TTTCAATAACAACACCATGT+TGG	-	chr5_2:18482055-18482074	None:intergenic	30.0%
TGAGCATTTGAATTATTCTG+TGG	+	chr5_2:18482212-18482231	Msa0761300:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	18479910	18483549	18479910	ID=Msa0761300;Name=Msa0761300
chr5_2	mRNA	18479910	18483549	18479910	ID=Msa0761300-mRNA-1;Parent=Msa0761300;Name=Msa0761300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|1\|0.5\|1\|1\|1\|2\|319\|457
chr5_2	exon	18479910	18480521	18479910	ID=Msa0761300-mRNA-1:exon:8009;Parent=Msa0761300-mRNA-1
chr5_2	exon	18482469	18483549	18482469	ID=Msa0761300-mRNA-1:exon:8010;Parent=Msa0761300-mRNA-1
chr5_2	CDS	18479910	18480521	18479910	ID=Msa0761300-mRNA-1:cds;Parent=Msa0761300-mRNA-1
chr5_2	CDS	18482469	18483230	18482469	ID=Msa0761300-mRNA-1:cds;Parent=Msa0761300-mRNA-1
chr5_2	three_prime_UTR	18483231	18483549	18483231	ID=Msa0761300-mRNA-1:three_prime_utr;Parent=Msa0761300-mRNA-1

Gene Sequence

>Msa0761300

ATGCCAAACAGAAACCCCACTGTCGTTTACCCACCTGGCACCCCACGAAGATCACCGAGACTCATCCTCCTCCATGAAAACAACAACAACCCCACCACCCCAAAACCCAAATCTACAACAAAATCATCTTCAACCATATCAAGATCCAAAACCGTTAGGGTAAGAAAATCACCCAATTTGAGCGATCAAGTTCCGTCGCTTAGACGGTCTCCTAGATTCTCCAAGAAAAATGATGTTAAAGTGAGTGAATTTGGTTCCTGTGATTCGATGAGAAAAACAGGTCGTGAAGGAAAAGAGAAGAAAAGTAGCGGGGGAGTTGTGGTTGAAGGTGGTAGAAAAAGGAGAAGAAAGCACGGTGGTGATGGAATTGAGGAAGGGAGGAAGAAAGAAGAGATTGAAGGTGGAGTAGAAGGGAAAAGGAAACACGATGGTGGTGGTGAAATAGTGGAAGAGTTGAGGAAGGAAGAAGTTGAATGTGGTGTGAAAAGGAAGAGAAAGCGCGGTGGTGGTGAAGTTGCACAAGGGTGGACTAAGGAACAAGAATTGGCTTTGAGAACAGCTTATTTTACTGCAAAACCTAGTCCTCATTTCTGGAAGAACGTTTCTAAACTGGTACCTGGGAAGTCTAAACAAGATTGCTTCGATAGAGTTCACCACGACTTCCACACACCACCTCAATGTCAACCTCGATCAAGGGCAAAGGCAATTAACTCATCACCCCTTCATCAATTCTCAATATCTCCAAGTAAACTGCTCATACCCACTGGAAAGAAGGTTGGCAAATCAAATATTCTAAAACCAAAGAGCATTGTTACTCAGAAGTCTATTGAGAATCTATTACAGTGCCATCTTAAAGTTGATCAAAATCATAAAGGGGACATATTTTCTGTTCTTGAACCCAACTTTGATTTTTCTACTAATGCTATTCTGCCTAGTCAAGAACTTTGTACTCCAAAGCAACAAAAGGAAAATCAAGGGTTCCTACAAATTTGCAATGAAAGATCATCATCATCTTCAATTCATAAGAAGTCTCTTTCTAGATTTAGTGGCTCATCAGGTGTTAAAGATCTTGCTAGTCCACCTGTGCTAAAGCAAGTAAAAAACAAGGTGCAGCATGAAAAATATGTCAATCAATTAAGATTTAGGGACCTTAGGAGAAGAGCAGCATCTTCAAGGGCAAAAAAATCTGTAGCTGGAGAAGGAAAAGGCATTCAAAAAACAGATGTTGTTAAAGCAGCAAAAGTTGCATTGGTTTCTGAAGCTAGAGATGCTATCAATAAATTTCAGCAATCCCAAATCAATTTCATGGGCGACACTTGTAGTTCTGATGAGGACAATGACGATGACACTGGAGCTGAAGATGAAACTCCATAG

Protein sequence

>Msa0761300

MPNRNPTVVYPPGTPRRSPRLILLHENNNNPTTPKPKSTTKSSSTISRSKTVRVRKSPNLSDQVPSLRRSPRFSKKNDVKVSEFGSCDSMRKTGREGKEKKSSGGVVVEGGRKRRRKHGGDGIEEGRKKEEIEGGVEGKRKHDGGGEIVEELRKEEVECGVKRKRKRGGGEVAQGWTKEQELALRTAYFTAKPSPHFWKNVSKLVPGKSKQDCFDRVHHDFHTPPQCQPRSRAKAINSSPLHQFSISPSKLLIPTGKKVGKSNILKPKSIVTQKSIENLLQCHLKVDQNHKGDIFSVLEPNFDFSTNAILPSQELCTPKQQKENQGFLQICNERSSSSSIHKKSLSRFSGSSGVKDLASPPVLKQVKNKVQHEKYVNQLRFRDLRRRAASSRAKKSVAGEGKGIQKTDVVKAAKVALVSEARDAINKFQQSQINFMGDTCSSDEDNDDDTGAEDETP*