Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0764060 | XP_013453735.1 | 75.362 | 69 | 13 | 1 | 1 | 65 | 310 | 378 | 6.46e-26 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0764060 | A0A072UEA2 | 75.362 | 69 | 13 | 1 | 1 | 65 | 310 | 378 | 3.09e-26 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0054250 | Msa0764060 | 0.802264 | 6.092355e-49 | -8.615850e-47 |
| Msa0054260 | Msa0764060 | 0.804522 | 2.074508e-49 | -8.615850e-47 |
| Msa0055690 | Msa0764060 | 0.839566 | 1.468003e-57 | -8.615850e-47 |
| Msa0100080 | Msa0764060 | 0.804658 | 1.943278e-49 | -8.615850e-47 |
| Msa0100090 | Msa0764060 | 0.805285 | 1.436991e-49 | -8.615850e-47 |
| Msa0680060 | Msa0764060 | 0.829147 | 6.058085e-55 | -8.615850e-47 |
| Msa0764060 | Msa1006630 | 0.801823 | 7.507822e-49 | -8.615850e-47 |
| Msa0764060 | Msa1024530 | 0.805145 | 1.537638e-49 | -8.615850e-47 |
| Msa0764060 | Msa1027290 | 0.803444 | 3.476388e-49 | -8.615850e-47 |
| Msa0764060 | Msa1381960 | 0.804674 | 1.928926e-49 | -8.615850e-47 |
| Msa0141250 | Msa0764060 | 0.813843 | 2.085055e-51 | -8.615850e-47 |
| Msa0187710 | Msa0764060 | 0.837945 | 3.852988e-57 | -8.615850e-47 |
| Msa0389280 | Msa0764060 | 0.804630 | 1.970272e-49 | -8.615850e-47 |
| Msa0398380 | Msa0764060 | 0.805904 | 1.065679e-49 | -8.615850e-47 |
| Msa0445280 | Msa0764060 | 0.810364 | 1.195764e-50 | -8.615850e-47 |
| Msa0314120 | Msa0764060 | 0.800849 | 1.187989e-48 | -8.615850e-47 |
| Msa0317510 | Msa0764060 | 0.802434 | 5.621534e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0764060 | MtrunA17_Chr5g0414371 | 75.362 | 69 | 13 | 1 | 1 | 65 | 310 | 378 | 5.94e-30 | 108 |
| Msa0764060 | MtrunA17_Chr5g0414441 | 92.453 | 53 | 4 | 0 | 1 | 53 | 318 | 370 | 7.61e-29 | 105 |
| Msa0764060 | MtrunA17_Chr5g0414391 | 84.906 | 53 | 8 | 0 | 1 | 53 | 312 | 364 | 1.21e-26 | 99.8 |
| Msa0764060 | MtrunA17_Chr5g0414421 | 86.792 | 53 | 7 | 0 | 1 | 53 | 347 | 399 | 1.47e-26 | 99.8 |
| Msa0764060 | MtrunA17_Chr5g0414461 | 84.906 | 53 | 8 | 0 | 1 | 53 | 311 | 363 | 1.49e-26 | 99.4 |
| Msa0764060 | MtrunA17_Chr0c01g0489311 | 58.621 | 58 | 19 | 2 | 1 | 57 | 329 | 382 | 3.27e-14 | 65.5 |
| Msa0764060 | MtrunA17_Chr6g0456421 | 52.830 | 53 | 25 | 0 | 1 | 53 | 329 | 381 | 7.30e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAATAAGTGGAAGAAATT+TGG | 0.277030 | 5_2:+23496998 | Msa0764060:CDS |
| GAGGAGAATGGATACAAAAT+TGG | 0.283501 | 5_2:+23497051 | Msa0764060:CDS |
| AAATGGGAACAAGAAAGATT+TGG | 0.385031 | 5_2:+23496917 | Msa0764060:CDS |
| GAAAGATTTGGATGGAATGC+TGG | 0.400495 | 5_2:+23496929 | Msa0764060:CDS |
| AAGTTATTCTTTGAGGAGAA+TGG | 0.403630 | 5_2:+23497039 | Msa0764060:CDS |
| ACAAAAGTTGAGGAGTTTGA+TGG | 0.425344 | 5_2:+23496974 | Msa0764060:CDS |
| TGGTTGAAAGTTATTCTTTG+AGG | 0.464463 | 5_2:+23497032 | Msa0764060:CDS |
| AGTTCTTGAAAGCATGGAAA+TGG | 0.475618 | 5_2:+23497930 | Msa0764060:CDS |
| TTTGGATGGAATGCTGGGAA+TGG | 0.504148 | 5_2:+23496935 | Msa0764060:CDS |
| AGGAACCGCTGCATCGGTAA+AGG | 0.507102 | 5_2:+23497907 | Msa0764060:CDS |
| AAAGATTTGGATGGAATGCT+GGG | 0.513249 | 5_2:+23496930 | Msa0764060:CDS |
| TGGTTGTTGAGGCAATGAAA+TGG | 0.519808 | 5_2:+23496900 | None:intergenic |
| GGAGTATTAAAGACTCCCTC+AGG | 0.545898 | 5_2:+23497887 | Msa0764060:CDS |
| TCCCTCAGGAACCGCTGCAT+CGG | 0.547515 | 5_2:+23497901 | Msa0764060:CDS |
| TACCGATGCAGCGGTTCCTG+AGG | 0.548069 | 5_2:-23497903 | None:intergenic |
| AGTTTGATGGAGCAAATAAG+TGG | 0.549427 | 5_2:+23496987 | Msa0764060:CDS |
| ACCGATGCAGCGGTTCCTGA+GGG | 0.563348 | 5_2:-23497902 | None:intergenic |
| TAAAGGAGTTCTTGAAAGCA+TGG | 0.563931 | 5_2:+23497924 | Msa0764060:CDS |
| GGTTGTTGAGGCAATGAAAT+GGG | 0.566403 | 5_2:+23496901 | None:intergenic |
| GGGAACAAGAAAGATTTGGA+TGG | 0.577539 | 5_2:+23496921 | Msa0764060:CDS |
| GAAATTTGGTAGTTATGTGT+TGG | 0.582462 | 5_2:+23497012 | Msa0764060:CDS |
| AGTGAGAGTAACAAAAGTTG+AGG | 0.593305 | 5_2:+23496964 | Msa0764060:CDS |
| GAACTCCTTTACCGATGCAG+CGG | 0.637351 | 5_2:-23497912 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATATATTATATTTATAA+AGG | + | chr5_2:23497539-23497558 | Msa0764060:intron | 0.0% |
| !! | AATATATATTATATTTATAA+AGG | + | chr5_2:23497539-23497558 | Msa0764060:intron | 0.0% |
| !! | AAAAAATTTACTTTAGTAAT+TGG | + | chr5_2:23497137-23497156 | Msa0764060:intron | 10.0% |
| !! | ATAAAAAACATACTATTTAA+AGG | - | chr5_2:23497241-23497260 | None:intergenic | 10.0% |
| !! | GAAATTAATTAGTATAAAAA+TGG | - | chr5_2:23497574-23497593 | None:intergenic | 10.0% |
| !! | AAAAAATTTACTTTAGTAAT+TGG | + | chr5_2:23497137-23497156 | Msa0764060:intron | 10.0% |
| !! | ATAAAAAACATACTATTTAA+AGG | - | chr5_2:23497241-23497260 | None:intergenic | 10.0% |
| !! | GAAATTAATTAGTATAAAAA+TGG | - | chr5_2:23497574-23497593 | None:intergenic | 10.0% |
| !! | TTATTGTGTATTCTTAATAT+TGG | + | chr5_2:23497406-23497425 | Msa0764060:intron | 15.0% |
| !!! | TAATTAATTTCGAATTTTGA+TGG | + | chr5_2:23497583-23497602 | Msa0764060:intron | 15.0% |
| !! | TTATTGTGTATTCTTAATAT+TGG | + | chr5_2:23497406-23497425 | Msa0764060:intron | 15.0% |
| !!! | TAATTAATTTCGAATTTTGA+TGG | + | chr5_2:23497583-23497602 | Msa0764060:intron | 15.0% |
| !! | TTAAGTATAGTATCCTTAAA+AGG | + | chr5_2:23497328-23497347 | Msa0764060:intron | 20.0% |
| !!! | TTTTATTTTGTGTTTCTAAG+AGG | + | chr5_2:23497471-23497490 | Msa0764060:intron | 20.0% |
| !! | TTAAGTATAGTATCCTTAAA+AGG | + | chr5_2:23497328-23497347 | Msa0764060:intron | 20.0% |
| !!! | TTTTATTTTGTGTTTCTAAG+AGG | + | chr5_2:23497471-23497490 | Msa0764060:intron | 20.0% |
| !! | AATGTTAAGTTGTTAATGAG+TGG | + | chr5_2:23497196-23497215 | Msa0764060:intron | 25.0% |
| ! | ATAAAATACCCAAAATGTAG+TGG | - | chr5_2:23497457-23497476 | None:intergenic | 25.0% |
| ! | ACTTCATTTGTAGTAAAAGA+CGG | + | chr5_2:23497751-23497770 | Msa0764060:intron | 25.0% |
| !! | AATGTTAAGTTGTTAATGAG+TGG | + | chr5_2:23497196-23497215 | Msa0764060:intron | 25.0% |
| ! | ATAAAATACCCAAAATGTAG+TGG | - | chr5_2:23497457-23497476 | None:intergenic | 25.0% |
| ! | ACTTCATTTGTAGTAAAAGA+CGG | + | chr5_2:23497751-23497770 | Msa0764060:intron | 25.0% |
| AAATGGGAACAAGAAAGATT+TGG | + | chr5_2:23496917-23496936 | Msa0764060:CDS | 30.0% | |
| GCAAATAAGTGGAAGAAATT+TGG | + | chr5_2:23496998-23497017 | Msa0764060:CDS | 30.0% | |
| ! | GAAATTTGGTAGTTATGTGT+TGG | + | chr5_2:23497012-23497031 | Msa0764060:CDS | 30.0% |
| TGGTTGAAAGTTATTCTTTG+AGG | + | chr5_2:23497032-23497051 | Msa0764060:CDS | 30.0% | |
| ! | AAGTTATTCTTTGAGGAGAA+TGG | + | chr5_2:23497039-23497058 | Msa0764060:CDS | 30.0% |
| !! | TATTTTGTGTTTCTAAGAGG+AGG | + | chr5_2:23497474-23497493 | Msa0764060:intron | 30.0% |
| ! | ACATTTTTCACATTTGTGCA+TGG | - | chr5_2:23497834-23497853 | None:intergenic | 30.0% |
| ATATACTGAACTGTATAAGC+AGG | + | chr5_2:23497861-23497880 | Msa0764060:intron | 30.0% | |
| AAATGGGAACAAGAAAGATT+TGG | + | chr5_2:23496917-23496936 | Msa0764060:CDS | 30.0% | |
| GCAAATAAGTGGAAGAAATT+TGG | + | chr5_2:23496998-23497017 | Msa0764060:CDS | 30.0% | |
| ! | GAAATTTGGTAGTTATGTGT+TGG | + | chr5_2:23497012-23497031 | Msa0764060:CDS | 30.0% |
| TGGTTGAAAGTTATTCTTTG+AGG | + | chr5_2:23497032-23497051 | Msa0764060:CDS | 30.0% | |
| ! | AAGTTATTCTTTGAGGAGAA+TGG | + | chr5_2:23497039-23497058 | Msa0764060:CDS | 30.0% |
| !! | TATTTTGTGTTTCTAAGAGG+AGG | + | chr5_2:23497474-23497493 | Msa0764060:intron | 30.0% |
| ! | ACATTTTTCACATTTGTGCA+TGG | - | chr5_2:23497834-23497853 | None:intergenic | 30.0% |
| ATATACTGAACTGTATAAGC+AGG | + | chr5_2:23497861-23497880 | Msa0764060:intron | 30.0% | |
| AAAGATTTGGATGGAATGCT+GGG | + | chr5_2:23496930-23496949 | Msa0764060:CDS | 35.0% | |
| AGTGAGAGTAACAAAAGTTG+AGG | + | chr5_2:23496964-23496983 | Msa0764060:CDS | 35.0% | |
| ACAAAAGTTGAGGAGTTTGA+TGG | + | chr5_2:23496974-23496993 | Msa0764060:CDS | 35.0% | |
| AGTTTGATGGAGCAAATAAG+TGG | + | chr5_2:23496987-23497006 | Msa0764060:CDS | 35.0% | |
| GAGGAGAATGGATACAAAAT+TGG | + | chr5_2:23497051-23497070 | Msa0764060:CDS | 35.0% | |
| ATAGACACTCACATCAAGTT+AGG | + | chr5_2:23497089-23497108 | Msa0764060:intron | 35.0% | |
| ACATCAAGTTAGGAGCAAAT+GGG | + | chr5_2:23497099-23497118 | Msa0764060:intron | 35.0% | |
| GAATTTGAGTGATGAAACCA+TGG | - | chr5_2:23497658-23497677 | None:intergenic | 35.0% | |
| ! | TTTTTCACATTTGTGCATGG+CGG | - | chr5_2:23497831-23497850 | None:intergenic | 35.0% |
| TAAAGGAGTTCTTGAAAGCA+TGG | + | chr5_2:23497924-23497943 | Msa0764060:CDS | 35.0% | |
| ! | AGTTCTTGAAAGCATGGAAA+TGG | + | chr5_2:23497930-23497949 | Msa0764060:CDS | 35.0% |
| AAAGATTTGGATGGAATGCT+GGG | + | chr5_2:23496930-23496949 | Msa0764060:CDS | 35.0% | |
| AGTGAGAGTAACAAAAGTTG+AGG | + | chr5_2:23496964-23496983 | Msa0764060:CDS | 35.0% | |
| ACAAAAGTTGAGGAGTTTGA+TGG | + | chr5_2:23496974-23496993 | Msa0764060:CDS | 35.0% | |
| AGTTTGATGGAGCAAATAAG+TGG | + | chr5_2:23496987-23497006 | Msa0764060:CDS | 35.0% | |
| GAGGAGAATGGATACAAAAT+TGG | + | chr5_2:23497051-23497070 | Msa0764060:CDS | 35.0% | |
| ATAGACACTCACATCAAGTT+AGG | + | chr5_2:23497089-23497108 | Msa0764060:intron | 35.0% | |
| ACATCAAGTTAGGAGCAAAT+GGG | + | chr5_2:23497099-23497118 | Msa0764060:intron | 35.0% | |
| GAATTTGAGTGATGAAACCA+TGG | - | chr5_2:23497658-23497677 | None:intergenic | 35.0% | |
| ! | TTTTTCACATTTGTGCATGG+CGG | - | chr5_2:23497831-23497850 | None:intergenic | 35.0% |
| TAAAGGAGTTCTTGAAAGCA+TGG | + | chr5_2:23497924-23497943 | Msa0764060:CDS | 35.0% | |
| ! | AGTTCTTGAAAGCATGGAAA+TGG | + | chr5_2:23497930-23497949 | Msa0764060:CDS | 35.0% |
| GGGAACAAGAAAGATTTGGA+TGG | + | chr5_2:23496921-23496940 | Msa0764060:CDS | 40.0% | |
| GAAAGATTTGGATGGAATGC+TGG | + | chr5_2:23496929-23496948 | Msa0764060:CDS | 40.0% | |
| CACATCAAGTTAGGAGCAAA+TGG | + | chr5_2:23497098-23497117 | Msa0764060:intron | 40.0% | |
| AAAGGCCTTCAATAGTGTCA+CGG | - | chr5_2:23497223-23497242 | None:intergenic | 40.0% | |
| !!! | TGTCTTTGGGACACCTTTTA+AGG | - | chr5_2:23497344-23497363 | None:intergenic | 40.0% |
| AACGACTGTCTCTTGTCTTT+GGG | - | chr5_2:23497357-23497376 | None:intergenic | 40.0% | |
| TAACGACTGTCTCTTGTCTT+TGG | - | chr5_2:23497358-23497377 | None:intergenic | 40.0% | |
| ACAAGAGACAGTCGTTAACA+AGG | + | chr5_2:23497361-23497380 | Msa0764060:intron | 40.0% | |
| CAAGAGACAGTCGTTAACAA+GGG | + | chr5_2:23497362-23497381 | Msa0764060:intron | 40.0% | |
| ! | TGATGTGAGCCACTACATTT+TGG | + | chr5_2:23497445-23497464 | Msa0764060:intron | 40.0% |
| ! | GATGTGAGCCACTACATTTT+GGG | + | chr5_2:23497446-23497465 | Msa0764060:intron | 40.0% |
| GTGTAATCACTAATCAGCCA+TGG | + | chr5_2:23497638-23497657 | Msa0764060:intron | 40.0% | |
| ACTGAACTGTATAAGCAGGA+TGG | + | chr5_2:23497865-23497884 | Msa0764060:intron | 40.0% | |
| CTGAACTGTATAAGCAGGAT+GGG | + | chr5_2:23497866-23497885 | Msa0764060:intron | 40.0% | |
| GGGAACAAGAAAGATTTGGA+TGG | + | chr5_2:23496921-23496940 | Msa0764060:CDS | 40.0% | |
| GAAAGATTTGGATGGAATGC+TGG | + | chr5_2:23496929-23496948 | Msa0764060:CDS | 40.0% | |
| CACATCAAGTTAGGAGCAAA+TGG | + | chr5_2:23497098-23497117 | Msa0764060:intron | 40.0% | |
| AAAGGCCTTCAATAGTGTCA+CGG | - | chr5_2:23497223-23497242 | None:intergenic | 40.0% | |
| !!! | TGTCTTTGGGACACCTTTTA+AGG | - | chr5_2:23497344-23497363 | None:intergenic | 40.0% |
| AACGACTGTCTCTTGTCTTT+GGG | - | chr5_2:23497357-23497376 | None:intergenic | 40.0% | |
| TAACGACTGTCTCTTGTCTT+TGG | - | chr5_2:23497358-23497377 | None:intergenic | 40.0% | |
| ACAAGAGACAGTCGTTAACA+AGG | + | chr5_2:23497361-23497380 | Msa0764060:intron | 40.0% | |
| CAAGAGACAGTCGTTAACAA+GGG | + | chr5_2:23497362-23497381 | Msa0764060:intron | 40.0% | |
| ! | TGATGTGAGCCACTACATTT+TGG | + | chr5_2:23497445-23497464 | Msa0764060:intron | 40.0% |
| ! | GATGTGAGCCACTACATTTT+GGG | + | chr5_2:23497446-23497465 | Msa0764060:intron | 40.0% |
| GTGTAATCACTAATCAGCCA+TGG | + | chr5_2:23497638-23497657 | Msa0764060:intron | 40.0% | |
| ACTGAACTGTATAAGCAGGA+TGG | + | chr5_2:23497865-23497884 | Msa0764060:intron | 40.0% | |
| CTGAACTGTATAAGCAGGAT+GGG | + | chr5_2:23497866-23497885 | Msa0764060:intron | 40.0% | |
| TTTGGATGGAATGCTGGGAA+TGG | + | chr5_2:23496935-23496954 | Msa0764060:CDS | 45.0% | |
| ! | GGAGTATTAAAGACTCCCTC+AGG | + | chr5_2:23497887-23497906 | Msa0764060:CDS | 45.0% |
| TTTGGATGGAATGCTGGGAA+TGG | + | chr5_2:23496935-23496954 | Msa0764060:CDS | 45.0% | |
| ! | GGAGTATTAAAGACTCCCTC+AGG | + | chr5_2:23497887-23497906 | Msa0764060:CDS | 45.0% |
| !! | AAATTAATTAGTATAAAAAT+GGG | - | chr5_2:23497573-23497592 | None:intergenic | 5.0% |
| !! | AAATTAATTAGTATAAAAAT+GGG | - | chr5_2:23497573-23497592 | None:intergenic | 5.0% |
| GTGGTCCGTGACACTATTGA+AGG | + | chr5_2:23497215-23497234 | Msa0764060:intron | 50.0% | |
| GAACTCCTTTACCGATGCAG+CGG | - | chr5_2:23497915-23497934 | None:intergenic | 50.0% | |
| GTGGTCCGTGACACTATTGA+AGG | + | chr5_2:23497215-23497234 | Msa0764060:intron | 50.0% | |
| GAACTCCTTTACCGATGCAG+CGG | - | chr5_2:23497915-23497934 | None:intergenic | 50.0% | |
| ! | AGGAACCGCTGCATCGGTAA+AGG | + | chr5_2:23497907-23497926 | Msa0764060:CDS | 55.0% |
| ! | AGGAACCGCTGCATCGGTAA+AGG | + | chr5_2:23497907-23497926 | Msa0764060:CDS | 55.0% |
| TCCCTCAGGAACCGCTGCAT+CGG | + | chr5_2:23497901-23497920 | Msa0764060:CDS | 60.0% | |
| ACCGATGCAGCGGTTCCTGA+GGG | - | chr5_2:23497905-23497924 | None:intergenic | 60.0% | |
| TACCGATGCAGCGGTTCCTG+AGG | - | chr5_2:23497906-23497925 | None:intergenic | 60.0% | |
| TCCCTCAGGAACCGCTGCAT+CGG | + | chr5_2:23497901-23497920 | Msa0764060:CDS | 60.0% | |
| ACCGATGCAGCGGTTCCTGA+GGG | - | chr5_2:23497905-23497924 | None:intergenic | 60.0% | |
| TACCGATGCAGCGGTTCCTG+AGG | - | chr5_2:23497906-23497925 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 23496914 | 23497960 | 23496914 | ID=Msa0764060;Name=Msa0764060 |
| chr5_2 | mRNA | 23496914 | 23497960 | 23496914 | ID=Msa0764060-mRNA-1;Parent=Msa0764060;Name=Msa0764060-mRNA-1;_AED=0.49;_eAED=0.65;_QI=0|0|0|1|1|1|2|0|78 |
| chr5_2 | exon | 23496914 | 23497072 | 23496914 | ID=Msa0764060-mRNA-1:exon:9714;Parent=Msa0764060-mRNA-1 |
| chr5_2 | exon | 23497883 | 23497960 | 23497883 | ID=Msa0764060-mRNA-1:exon:9715;Parent=Msa0764060-mRNA-1 |
| chr5_2 | CDS | 23496914 | 23497072 | 23496914 | ID=Msa0764060-mRNA-1:cds;Parent=Msa0764060-mRNA-1 |
| chr5_2 | CDS | 23497883 | 23497960 | 23497883 | ID=Msa0764060-mRNA-1:cds;Parent=Msa0764060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
