AlfalfaGEDB | Msa0100090

M. sativa cultivar zhongmu-4 / Msa0100090

KEGG: -
GO: GO:0001101 GO:0002237 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0100090	sp\|Q9SAH7\|WRK40_ARATH	36.996	273	135	5	2	237	27	299	2.60e-44	154

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0100090	G7I7N0	91.270	252	20	2	1	251	5	255	2.67e-169	476

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TFs/TRs:

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Gene ID	Type	Classification
Msa0100090	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0100090	0.806103	9.677710e-50	-8.615850e-47
Msa0006070	Msa0100090	0.803157	3.985355e-49	-8.615850e-47
Msa0007080	Msa0100090	0.807175	5.747584e-50	-8.615850e-47
Msa0007280	Msa0100090	0.851367	9.291961e-61	-8.615850e-47
Msa0007440	Msa0100090	0.800131	1.664479e-48	-8.615850e-47
Msa0007990	Msa0100090	0.817305	3.533487e-52	-8.615850e-47
Msa0010890	Msa0100090	0.801558	8.509329e-49	-8.615850e-47
Msa0011170	Msa0100090	0.804035	2.620827e-49	-8.615850e-47
Msa0012040	Msa0100090	0.812328	4.480810e-51	-8.615850e-47
Msa0014710	Msa0100090	0.819683	1.021340e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0100090	MtrunA17_Chr1g0150271	91.270	252	20	2	1	251	5	255	5.15e-173	476
Msa0100090	MtrunA17_Chr1g0150281	46.626	163	74	4	78	234	122	277	7.45e-42	144
Msa0100090	MtrunA17_Chr4g0001361	36.466	266	131	4	2	229	45	310	1.81e-41	144
Msa0100090	MtrunA17_Chr2g0333851	37.121	264	123	6	2	229	53	309	7.13e-40	140
Msa0100090	MtrunA17_Chr3g0136961	41.250	160	83	2	81	229	123	282	2.41e-37	132
Msa0100090	MtrunA17_Chr7g0270831	38.378	185	82	6	2	156	94	276	1.87e-30	117
Msa0100090	MtrunA17_Chr3g0099641	48.421	95	47	1	78	172	235	327	7.90e-27	107
Msa0100090	MtrunA17_Chr4g0060271	52.439	82	37	1	75	156	236	315	2.37e-25	104
Msa0100090	MtrunA17_Chr4g0051181	42.342	111	52	2	56	156	195	303	3.19e-25	103
Msa0100090	MtrunA17_Chr2g0288871	54.795	73	33	0	84	156	293	365	1.90e-24	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0100090	AT1G80840.1	36.996	273	135	5	2	237	27	299	2.64e-45	154
Msa0100090	AT4G31800.2	49.351	154	70	2	82	235	97	242	3.46e-42	144
Msa0100090	AT4G31800.1	49.351	154	70	2	82	235	162	307	1.16e-41	145
Msa0100090	AT2G25000.1	36.923	260	114	8	2	239	39	270	2.20e-37	132
Msa0100090	AT2G25000.2	42.623	183	77	6	57	239	73	227	4.86e-37	130
Msa0100090	AT2G25000.4	42.623	183	77	6	57	239	73	227	4.86e-37	130
Msa0100090	AT2G25000.3	43.169	183	76	6	57	239	95	249	2.10e-36	129
Msa0100090	AT4G04450.2	47.191	89	47	0	84	172	186	274	4.36e-26	105
Msa0100090	AT4G04450.1	47.191	89	47	0	84	172	280	368	1.12e-25	105
Msa0100090	AT4G22070.4	56.164	73	32	0	84	156	285	357	3.10e-25	104

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Find 59 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTACTTGCGACGTTT+GGG	0.370540	1_3:+6569799	Msa0100090:three_prime_UTR
AAATCTCGATTTGAATCTTT+CGG	0.326816	1_3:+6569638	Msa0100090:CDS
AATAAGTTGAAAATGGCTTA+TGG	0.337085	1_3:-6569901	None:intergenic
AATATATAGATAATAAAAGA+TGG	0.494453	1_3:+6569008	Msa0100090:intron
AATTCTTTACACTTGTATTT+TGG	0.191740	1_3:-6569698	None:intergenic
ACAAGTTCAAATCATCAAAT+TGG	0.204407	1_3:+6568640	Msa0100090:CDS
ACTTAGATAGTATCAAAATA+TGG	0.392382	1_3:+6569731	Msa0100090:three_prime_UTR
AGAGTTACAACAAGTGAAAG+AGG	0.509069	1_3:+6568519	Msa0100090:CDS
AGCTGTTATAATGGATGAAA+AGG	0.418939	1_3:+6568315	None:intergenic
AGCTTACTTTAGGTGCTCCA+TGG	0.512350	1_3:+6569085	Msa0100090:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAACCTAATGATGAAAGTT+TGG	+	chr1_3:6568747-6568766	Msa0100090:CDS	30.0%
CATACCAAACTTTCATCATT+AGG	-	chr1_3:6568754-6568773	None:intergenic	30.0%
GATGGTTTAAATGACTCATT+AGG	-	chr1_3:6569381-6569400	None:intergenic	30.0%
TTAGAGATTGATGAACCTTT+AGG	-	chr1_3:6569414-6569433	None:intergenic	30.0%
TCTTTGTCATTCAATGTTGT+TGG	-	chr1_3:6569447-6569466	None:intergenic	30.0%
AAAATTTACTTGCGACGTTT+GGG	+	chr1_3:6569799-6569818	Msa0100090:three_prime_UTR	30.0%
TCAACCTAATGATGAAAGTT+TGG	+	chr1_3:6568747-6568766	Msa0100090:CDS	30.0%
CATACCAAACTTTCATCATT+AGG	-	chr1_3:6568754-6568773	None:intergenic	30.0%
GATGGTTTAAATGACTCATT+AGG	-	chr1_3:6569381-6569400	None:intergenic	30.0%
TTAGAGATTGATGAACCTTT+AGG	-	chr1_3:6569414-6569433	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	6568325	6569962	6568325	ID=Msa0100090;Name=Msa0100090
chr1_3	mRNA	6568325	6569962	6568325	ID=Msa0100090-mRNA-1;Parent=Msa0100090;Name=Msa0100090-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0.66\|0.5\|0.75\|1\|1\|4\|294\|251
chr1_3	exon	6568325	6568336	6568325	ID=Msa0100090-mRNA-1:exon:2442;Parent=Msa0100090-mRNA-1
chr1_3	exon	6568502	6568768	6568502	ID=Msa0100090-mRNA-1:exon:2443;Parent=Msa0100090-mRNA-1
chr1_3	exon	6569017	6569133	6569017	ID=Msa0100090-mRNA-1:exon:2444;Parent=Msa0100090-mRNA-1
chr1_3	exon	6569309	6569962	6569309	ID=Msa0100090-mRNA-1:exon:2445;Parent=Msa0100090-mRNA-1
chr1_3	CDS	6568325	6568336	6568325	ID=Msa0100090-mRNA-1:cds;Parent=Msa0100090-mRNA-1
chr1_3	CDS	6568502	6568768	6568502	ID=Msa0100090-mRNA-1:cds;Parent=Msa0100090-mRNA-1
chr1_3	CDS	6569017	6569133	6569017	ID=Msa0100090-mRNA-1:cds;Parent=Msa0100090-mRNA-1
chr1_3	CDS	6569309	6569668	6569309	ID=Msa0100090-mRNA-1:cds;Parent=Msa0100090-mRNA-1
chr1_3	three_prime_UTR	6569669	6569962	6569669	ID=Msa0100090-mRNA-1:three_prime_utr;Parent=Msa0100090-mRNA-1

Gene Sequence

>Msa0100090

ATGGATGAAAAGGTGGAAACTCTTGAATTAGAGTTACAACAAGTGAAAGAGGAAAACAATACTCTAAGATTCATGCTTGAAGTTATGAATATCAAGTGTACAAAGCTTGAGTCCCATCTTCAAGAGATCAACAAGGAAGGACACAAAGGGACAAGTTCAAATCATCAAATTGGGTCAATACCTAACATTGATTCAAGCAAAAGAGCAAGACTAGAGATTCCCACAGCACAAAAGCCACTACAAGTCTTTGTACGAACTCAACCTAATGATGAAAGTTTGATAATAAAAGATGGCTATCAATGGAGGAAGTATGGTCAAAAGGTTACCAAAGACAATGCTTCTCCTAGAGCTTACTTTAGGTGCTCCATGGCTCCTAGTTGCCCAGCAAAAAAGAAGGTACAAAAATGCATACATGATAGGTCTATCCTTGTTGCAACATATGATGGAGAGCACAACCATGGTGTCCCTAATGAGTCATTTAAACCATCTTCATCCACACCTAAAGGTTCATCAATCTCTAATAATAAATTACCAACAACATTGAATGACAAAGAAGCCACAAATACTAGACTTTGTGAGAATGTCATGCAACAGTTTGGCTATGGAAGGCACATTAAAATTGAAGAATATGCAAGTTCCCTAATCAAAGATCCTGACTTCACTGCAGCATTAGCTGAAGCAGTTGCTCGAACCGTCACTGATCAAGAACATAAGCGACAAGGTCTAAATCTCGATTTGAATCTTTCGGAGGAGTGA

Protein sequence

>Msa0100090

MDEKVETLELELQQVKEENNTLRFMLEVMNIKCTKLESHLQEINKEGHKGTSSNHQIGSIPNIDSSKRARLEIPTAQKPLQVFVRTQPNDESLIIKDGYQWRKYGQKVTKDNASPRAYFRCSMAPSCPAKKKVQKCIHDRSILVATYDGEHNHGVPNESFKPSSSTPKGSSISNNKLPTTLNDKEATNTRLCENVMQQFGYGRHIKIEEYASSLIKDPDFTAALAEAVARTVTDQEHKRQGLNLDLNLSEE*