Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0811430 | XP_003615340.1 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 5.59e-51 | 165 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0811430 | G7JYH8 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 2.67e-51 | 165 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004840 | Msa0811430 | 0.839619 | 1.421711e-57 | -8.615850e-47 |
| Msa0038870 | Msa0811430 | 0.816465 | 5.453862e-52 | -8.615850e-47 |
| Msa0051860 | Msa0811430 | 0.825886 | 3.671080e-54 | -8.615850e-47 |
| Msa0068020 | Msa0811430 | 0.800619 | 1.324085e-48 | -8.615850e-47 |
| Msa0096980 | Msa0811430 | 0.835713 | 1.429530e-56 | -8.615850e-47 |
| Msa0673470 | Msa0811430 | 0.811025 | 8.605317e-51 | -8.615850e-47 |
| Msa0811430 | Msa0851620 | 0.996792 | 2.686585e-232 | -8.615850e-47 |
| Msa0811430 | Msa1043050 | 0.802643 | 5.088796e-49 | -8.615850e-47 |
| Msa0811430 | Msa1150710 | 0.802135 | 6.477084e-49 | -8.615850e-47 |
| Msa0811430 | Msa1176360 | 0.803308 | 3.709215e-49 | -8.615850e-47 |
| Msa0811430 | Msa1272620 | 0.809553 | 1.787487e-50 | -8.615850e-47 |
| Msa0811430 | Msa1423910 | 0.828806 | 7.327321e-55 | -8.615850e-47 |
| Msa0811430 | Msa1448630 | 0.801758 | 7.741837e-49 | -8.615850e-47 |
| Msa0145010 | Msa0811430 | 0.836653 | 8.249270e-57 | -8.615850e-47 |
| Msa0190120 | Msa0811430 | 0.804102 | 2.537636e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0811430 | MtrunA17_Chr5g0427841 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 5.14e-55 | 165 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0811430 | AT2G38465.1 | 59.420 | 69 | 28 | 0 | 19 | 87 | 13 | 81 | 1.94e-22 | 83.2 |
Find 13 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAATGTTAGAAGACTTAA+AGG | 0.245207 | 5_3:-40575849 | None:intergenic |
| AATCACAACAAAACTTCTTT+TGG | 0.285814 | 5_3:+40576037 | Msa0811430:CDS |
| GTCGGTGAACAATTGCTAAA+TGG | 0.339976 | 5_3:+40575805 | None:intergenic |
| ATATATATTTCATCTCTATA+AGG | 0.409482 | 5_3:+40575961 | Msa0811430:five_prime_UTR |
| CATTAATTTAATTAACTATG+AGG | 0.415967 | 5_3:-40576371 | None:intergenic |
| CTGCTGAACATATCAAGAAT+AGG | 0.429170 | 5_3:+40576339 | Msa0811430:CDS |
| AAAAGTTTCTTGTATAGAAA+CGG | 0.456929 | 5_3:-40576092 | None:intergenic |
| AAAAGTAGCTATGGAAGAGA+TGG | 0.471774 | 5_3:+40575988 | Msa0811430:exon |
| ATCTATAAAAGAAGGACAAA+TGG | 0.481664 | 5_3:+40575889 | Msa0811430:five_prime_UTR |
| TAAGGCAATAAAAGTAGCTA+TGG | 0.485068 | 5_3:+40575979 | Msa0811430:exon |
| AAAGTAGCTATGGAAGAGAT+GGG | 0.580832 | 5_3:+40575989 | Msa0811430:exon |
| GGCTTCAAAGAAACAAGTTG+AGG | 0.622095 | 5_3:+40576307 | Msa0811430:CDS |
| AGCAAGTGAAGCTGAACTTG+AGG | 0.634101 | 5_3:+40576286 | Msa0811430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATTTATCTATAAAAGA+AGG | + | chr5_3:40575881-40575900 | Msa0811430:five_prime_UTR | 10.0% |
| !! | ATATATATTTCATCTCTATA+AGG | + | chr5_3:40575961-40575980 | Msa0811430:five_prime_UTR | 15.0% |
| !! | CATTAATTTAATTAACTATG+AGG | - | chr5_3:40576374-40576393 | None:intergenic | 15.0% |
| !! | AAAAGTTTCTTGTATAGAAA+CGG | - | chr5_3:40576095-40576114 | None:intergenic | 20.0% |
| !! | AAATAAGTAGTAGCTAAAAA+TGG | - | chr5_3:40576177-40576196 | None:intergenic | 20.0% |
| ! | GATAATGTTAGAAGACTTAA+AGG | - | chr5_3:40575852-40575871 | None:intergenic | 25.0% |
| ! | ATCTATAAAAGAAGGACAAA+TGG | + | chr5_3:40575889-40575908 | Msa0811430:five_prime_UTR | 25.0% |
| !!! | AAAGAAGTTTTGTTGTGATT+TGG | - | chr5_3:40576037-40576056 | None:intergenic | 25.0% |
| !! | AATCACAACAAAACTTCTTT+TGG | + | chr5_3:40576037-40576056 | Msa0811430:CDS | 25.0% |
| ! | TAGTAGCTAAAAATGGAAAA+GGG | - | chr5_3:40576170-40576189 | None:intergenic | 25.0% |
| ! | GAATTGGTGTGAAAAAAAAT+AGG | + | chr5_3:40576232-40576251 | Msa0811430:intron | 25.0% |
| ! | TATAACTGCAAAAAATGAGA+AGG | + | chr5_3:40576262-40576281 | Msa0811430:CDS | 25.0% |
| TAAGGCAATAAAAGTAGCTA+TGG | + | chr5_3:40575979-40575998 | Msa0811430:exon | 30.0% | |
| !!! | TATGCAAAAGCCTTTTTTGT+CGG | + | chr5_3:40576118-40576137 | Msa0811430:CDS | 30.0% |
| !! | AAATGGAAAAGGGTTGTTTT+TGG | - | chr5_3:40576160-40576179 | None:intergenic | 30.0% |
| GTAGTAGCTAAAAATGGAAA+AGG | - | chr5_3:40576171-40576190 | None:intergenic | 30.0% | |
| ! | AACTTGTTTGATTGAGGAAT+TGG | + | chr5_3:40576216-40576235 | Msa0811430:intron | 30.0% |
| AAAAGAAAAACACTTGCATG+AGG | - | chr5_3:40576480-40576499 | None:intergenic | 30.0% | |
| ! | ATGCAAGTGTTTTTCTTTTC+TGG | + | chr5_3:40576481-40576500 | Msa0811430:three_prime_UTR | 30.0% |
| AAAAGTAGCTATGGAAGAGA+TGG | + | chr5_3:40575988-40576007 | Msa0811430:exon | 35.0% | |
| AAAGTAGCTATGGAAGAGAT+GGG | + | chr5_3:40575989-40576008 | Msa0811430:exon | 35.0% | |
| !! | GTTTTGTTGTGATTTGGCAA+AGG | - | chr5_3:40576031-40576050 | None:intergenic | 35.0% |
| AATTACCTGACCGACAAAAA+AGG | - | chr5_3:40576131-40576150 | None:intergenic | 35.0% | |
| CCTCAATCAAACAAGTTCAA+AGG | - | chr5_3:40576213-40576232 | None:intergenic | 35.0% | |
| ! | CCTTTGAACTTGTTTGATTG+AGG | + | chr5_3:40576210-40576229 | Msa0811430:intron | 35.0% |
| CTGCTGAACATATCAAGAAT+AGG | + | chr5_3:40576339-40576358 | Msa0811430:CDS | 35.0% | |
| !!! | AAAAGCCTTTTTTGTCGGTC+AGG | + | chr5_3:40576123-40576142 | Msa0811430:CDS | 40.0% |
| GGCTTCAAAGAAACAAGTTG+AGG | + | chr5_3:40576307-40576326 | Msa0811430:CDS | 40.0% | |
| AGCAAGTGAAGCTGAACTTG+AGG | + | chr5_3:40576286-40576305 | Msa0811430:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 40575810 | 40576541 | 40575810 | ID=Msa0811430;Name=Msa0811430 |
| chr5_3 | mRNA | 40575810 | 40576541 | 40575810 | ID=Msa0811430-mRNA-1;Parent=Msa0811430;Name=Msa0811430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=188|1|1|1|1|1|2|159|91 |
| chr5_3 | exon | 40575810 | 40576144 | 40575810 | ID=Msa0811430-mRNA-1:exon:12466;Parent=Msa0811430-mRNA-1 |
| chr5_3 | exon | 40576254 | 40576541 | 40576254 | ID=Msa0811430-mRNA-1:exon:12467;Parent=Msa0811430-mRNA-1 |
| chr5_3 | five_prime_UTR | 40575810 | 40575997 | 40575810 | ID=Msa0811430-mRNA-1:five_prime_utr;Parent=Msa0811430-mRNA-1 |
| chr5_3 | CDS | 40575998 | 40576144 | 40575998 | ID=Msa0811430-mRNA-1:cds;Parent=Msa0811430-mRNA-1 |
| chr5_3 | CDS | 40576254 | 40576382 | 40576254 | ID=Msa0811430-mRNA-1:cds;Parent=Msa0811430-mRNA-1 |
| chr5_3 | three_prime_UTR | 40576383 | 40576541 | 40576383 | ID=Msa0811430-mRNA-1:three_prime_utr;Parent=Msa0811430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
