AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0821070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821070 MtrunA17_Chr5g0441161 97.758 223 4 1 1 222 1 223 4.39e-156 431
Msa0821070 MtrunA17_Chr4g0008681 63.636 231 75 3 1 222 1 231 6.16e-76 229
Msa0821070 MtrunA17_Chr2g0305121 63.203 231 76 4 1 222 1 231 6.13e-74 224
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821070 AT5G12110.1 69.737 228 63 3 1 222 1 228 9.34e-100 289
Msa0821070 AT5G19510.1 74.107 224 56 2 1 222 1 224 1.62e-90 266
Msa0821070 AT2G18110.1 58.079 229 84 4 5 222 4 231 1.78e-72 220
Msa0821070 AT1G30230.1 59.211 228 83 4 5 222 4 231 3.83e-69 211
Msa0821070 AT1G30230.2 59.211 228 83 4 5 222 4 231 8.90e-69 212

Find 90 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCACCATCACTTCTTTAT+TGG 0.149755 5_3:-58860424 None:intergenic
GAGAGTTGCGTGTATGATTT+AGG 0.151326 5_3:+58857120 Msa0821070:five_prime_UTR
AATCGTCATATTAGCGTTCT+TGG 0.195713 5_3:+58860550 Msa0821070:three_prime_UTR
CTCTGGACATACTCGTTAAT+TGG 0.274565 5_3:-58860021 None:intergenic
GTCGTTTCTTCTTTCATGTT+TGG 0.292159 5_3:+58860150 Msa0821070:three_prime_UTR
TCTAATCAGCCGCTTGAAAA+TGG 0.306365 5_3:-58860089 None:intergenic
TTAGCAGGTGCTCCTGCAGC+TGG 0.312184 5_3:-58857982 None:intergenic
GTTTAAGTTCATGGTAATAA+TGG 0.313540 5_3:+58860484 Msa0821070:three_prime_UTR
TTACTCTCTTTCTTCTTTGC+GGG 0.314961 5_3:-58859481 None:intergenic
CTGAGAAGGTAACAGCCATT+AGG 0.352405 5_3:-58857259 None:intergenic
AGAGTTGCGTGTATGATTTA+GGG 0.369202 5_3:+58857121 Msa0821070:five_prime_UTR
TTGTGTATCGATTCAATAAA+CGG 0.373231 5_3:-58860338 None:intergenic
TGAGAAGGTAACAGCCATTA+GGG 0.375051 5_3:-58857258 None:intergenic
GATGATGATCTTGACCTCTT+TGG 0.382817 5_3:+58859403 Msa0821070:CDS
ACATGCTCCCCAGGTTAAAC+CGG 0.384492 5_3:-58859778 None:intergenic
TCCCTCAATGATTTCCTCTC+TGG 0.387083 5_3:+58857308 Msa0821070:CDS
TTGAAATTCCCGGTTTAACC+TGG 0.398982 5_3:+58859769 Msa0821070:CDS
TAATATGACGATTGTGCCTC+GGG 0.402753 5_3:-58860540 None:intergenic
CTTACTCTCTTTCTTCTTTG+CGG 0.404225 5_3:-58859482 None:intergenic
GTTCGCAGCGTTGAAATTCC+CGG 0.412225 5_3:+58859759 Msa0821070:CDS
CGATTCATCGCAAACCCTAA+TGG 0.420045 5_3:+58857244 Msa0821070:exon
AAACTTTAAATAGAAGCATA+TGG 0.431489 5_3:-58860298 None:intergenic
CAAAGTGTATGGATATGTTT+CGG 0.431607 5_3:+58857713 Msa0821070:CDS
AAGGATGATATCAAAGTGTA+TGG 0.446962 5_3:+58857702 Msa0821070:CDS
AGAAACGACAGTGGAGGAGA+AGG 0.452729 5_3:-58860136 None:intergenic
TAAGAAGGCAGCAGAGGAAA+GGG 0.453012 5_3:+58859444 Msa0821070:CDS
TTGGATTCCATTGTGTCACC+AGG 0.463893 5_3:+58860569 Msa0821070:three_prime_UTR
AGATACAAGATCGTCAATAA+TGG 0.472820 5_3:-58859962 None:intergenic
TGAAAGTTGAGAGAGTGTTT+TGG 0.473151 5_3:-58857183 None:intergenic
ATTGGCATGGCATATGCAAA+AGG 0.478361 5_3:-58860406 None:intergenic
CAAACGCCATGCACATTTGT+GGG 0.480562 5_3:+58860260 Msa0821070:three_prime_UTR
CATTAGGGTTTGCGATGAAT+CGG 0.480889 5_3:-58857243 None:intergenic
AGTGGAAGTGCTCCAGCTGC+AGG 0.481359 5_3:+58857970 Msa0821070:CDS
CCATCACTTCTTTATTGGCA+TGG 0.490430 5_3:-58860419 None:intergenic
AAGCTGGTTGCTGTTGGATA+CGG 0.492710 5_3:+58859913 Msa0821070:CDS
TCCAGAGAGGAAATCATTGA+GGG 0.494384 5_3:-58857309 None:intergenic
TCAAACGCCATGCACATTTG+TGG 0.499532 5_3:+58860259 Msa0821070:three_prime_UTR
GAAACGACAGTGGAGGAGAA+GGG 0.501856 5_3:-58860135 None:intergenic
TGAGACAGACATGAAGAAGC+TGG 0.511507 5_3:+58859728 Msa0821070:CDS
TTGCATTGTTACAGCCAAGC+TGG 0.512626 5_3:+58859897 Msa0821070:intron
ATATAAATACCAATTATTGA+AGG 0.513380 5_3:+58857160 Msa0821070:five_prime_UTR
TGAAACAGAGGAGGATAAGA+AGG 0.514826 5_3:+58859429 Msa0821070:CDS
ATAAGAAGGCAGCAGAGGAA+AGG 0.514986 5_3:+58859443 Msa0821070:CDS
TCATCATCATCTTCAGCAGC+AGG 0.517729 5_3:-58859388 None:intergenic
GCTGATTAGACTGTTATTTG+CGG 0.518900 5_3:+58860102 Msa0821070:three_prime_UTR
TTTCAACATACATGCTCCCC+AGG 0.520277 5_3:-58859787 None:intergenic
ATGCGACAATGTCACAGCTC+TGG 0.527719 5_3:-58860038 None:intergenic
ATGTCTGTCTCATCATCCCA+TGG 0.532053 5_3:-58859717 None:intergenic
ATTGTATGGAGTGAGAGAGA+TGG 0.536485 5_3:+58860202 Msa0821070:three_prime_UTR
TTCTGCTTGATGTTAAGCCA+TGG 0.537433 5_3:+58859700 Msa0821070:CDS
TCTCTTCACATCTTGCTGCA+AGG 0.539260 5_3:+58857784 Msa0821070:CDS
TGAAATTCCCGGTTTAACCT+GGG 0.541002 5_3:+58859770 Msa0821070:CDS
TGGCTTAACATCAAGCAGAA+TGG 0.546092 5_3:-58859697 None:intergenic
AGACATGAAGAAGCTGGAAG+AGG 0.547223 5_3:+58859734 Msa0821070:CDS
TTCCAGAGAGGAAATCATTG+AGG 0.548339 5_3:-58857310 None:intergenic
TCATGTTTGGTGTGTTTCAG+TGG 0.549318 5_3:+58860163 Msa0821070:three_prime_UTR
CTAATATGACGATTGTGCCT+CGG 0.550818 5_3:-58860541 None:intergenic
GAGATACATACAGCTTTAGC+AGG 0.553332 5_3:-58857997 None:intergenic
CTGTGAGTCTTTCTTCAATG+AGG 0.560347 5_3:-58859993 None:intergenic
ATCGTACCAACCAGCAACAT+TGG 0.561284 5_3:-58857757 None:intergenic
CTCAGATCTTCACACAGAAG+AGG 0.570425 5_3:+58857277 Msa0821070:CDS
ACATTGGCAAAAGCATCACT+TGG 0.572710 5_3:-58857741 None:intergenic
TATTGACGATCTTGTATCTG+TGG 0.575194 5_3:+58859966 Msa0821070:CDS
GGAGGATAAGAAGGCAGCAG+AGG 0.581848 5_3:+58859438 Msa0821070:CDS
TCAGATCTTCACACAGAAGA+GGG 0.583876 5_3:+58857278 Msa0821070:CDS
GCTCAAGGTGTGAAATTCAG+TGG 0.585505 5_3:+58857952 Msa0821070:CDS
AGTTTCCCTGGAAAAGCTCA+AGG 0.588553 5_3:+58857937 Msa0821070:intron
TCTGCTTGATGTTAAGCCAT+GGG 0.593895 5_3:+58859701 Msa0821070:CDS
TGTTTGGTGTGTTTCAGTGG+TGG 0.597058 5_3:+58860166 Msa0821070:three_prime_UTR
CATGCTCCCCAGGTTAAACC+GGG 0.597168 5_3:-58859777 None:intergenic
TAGGGTTTGCGATGAATCGG+TGG 0.599404 5_3:-58857240 None:intergenic
ACAGCCAAGCTGGTTGCTGT+TGG 0.600555 5_3:+58859907 Msa0821070:intron
GCCAATAAAGAAGTGATGGT+GGG 0.602763 5_3:+58860423 Msa0821070:three_prime_UTR
TTCTGTGTGAAGATCTGAGA+AGG 0.604138 5_3:-58857273 None:intergenic
GAAATTCCCGGTTTAACCTG+GGG 0.605534 5_3:+58859771 Msa0821070:CDS
ACTAGAGCCTGGTGACACAA+TGG 0.616385 5_3:-58860576 None:intergenic
CCTCTGTTTCATCACCAAAG+AGG 0.617705 5_3:-58859417 None:intergenic
GAAGGCAGCAGAGGAAAGGG+AGG 0.617890 5_3:+58859447 Msa0821070:CDS
GGTTTGCGATGAATCGGTGG+CGG 0.621249 5_3:-58857237 None:intergenic
TGCCAATAAAGAAGTGATGG+TGG 0.623466 5_3:+58860422 Msa0821070:three_prime_UTR
GTATCCAACAGCAACCAGCT+TGG 0.624167 5_3:-58859911 None:intergenic
ATGGTAATAATGGTTACATG+TGG 0.633867 5_3:+58860494 Msa0821070:three_prime_UTR
ATTCAGGGATCAATTGACAA+AGG 0.634362 5_3:+58857683 Msa0821070:intron
CCATGCCAATAAAGAAGTGA+TGG 0.634885 5_3:+58860419 Msa0821070:three_prime_UTR
CCTCTTTGGTGATGAAACAG+AGG 0.645851 5_3:+58859417 Msa0821070:CDS
ACGACAGTGGAGGAGAAGGG+AGG 0.647055 5_3:-58860132 None:intergenic
GAAAGAAGAAACGACAGTGG+AGG 0.651111 5_3:-58860142 None:intergenic
CTTTGGTGATGAAACAGAGG+AGG 0.675786 5_3:+58859420 Msa0821070:CDS
GCTAATCCCACAAATGTGCA+TGG 0.680283 5_3:-58860266 None:intergenic
CATGAAAGAAGAAACGACAG+TGG 0.723853 5_3:-58860145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAGAAAAAAAAATAATAT+TGG + chr5_3:58857098-58857117 Msa0821070:five_prime_UTR 10.0%
!! AATATAAAGATCAATAATTA+AGG - chr5_3:58857836-58857855 None:intergenic 10.0%
!! TTTATATTGAAATATGAATT+TGG + chr5_3:58857848-58857867 Msa0821070:intron 10.0%
!! AATTTCAATAATAAAATTGT+TGG - chr5_3:58858869-58858888 None:intergenic 10.0%
!! ATATAAATACCAATTATTGA+AGG + chr5_3:58857160-58857179 Msa0821070:five_prime_UTR 15.0%
!!! TTCTATTTTATTGTTTTGAA+TGG + chr5_3:58857616-58857635 Msa0821070:intron 15.0%
!!! TCTATTTTATTGTTTTGAAT+GGG + chr5_3:58857617-58857636 Msa0821070:intron 15.0%
!! CTGAAAGATTATTATTAATT+AGG + chr5_3:58858415-58858434 Msa0821070:intron 15.0%
!!! AAAATTTAAGTTTGAGTTTT+TGG + chr5_3:58860066-58860085 Msa0821070:exon 15.0%
!! ATGAACTTAAACTAAAAAAT+CGG - chr5_3:58860476-58860495 None:intergenic 15.0%
!!! AAATATATTTTTTTCCAGAG+AGG - chr5_3:58857325-58857344 None:intergenic 20.0%
!! TCTGGAAAAAAATATATTTC+TGG + chr5_3:58857326-58857345 Msa0821070:CDS 20.0%
!! CTGGAAAAAAATATATTTCT+GGG + chr5_3:58857327-58857346 Msa0821070:CDS 20.0%
!! TAAAAAAAGTAGAAATTGAG+AGG - chr5_3:58857365-58857384 None:intergenic 20.0%
!!! TTTTTATTGTTTCTGAGATT+TGG + chr5_3:58857409-58857428 Msa0821070:intron 20.0%
!!! TTTTATTGTTTCTGAGATTT+GGG + chr5_3:58857410-58857429 Msa0821070:intron 20.0%
!! ATTCGAATCAATAAAATAGA+AGG - chr5_3:58857468-58857487 None:intergenic 20.0%
!!! TTTTTTACTGTTTTGAGATT+TGG + chr5_3:58857507-58857526 Msa0821070:intron 20.0%
!!! TTTTTACTGTTTTGAGATTT+GGG + chr5_3:58857508-58857527 Msa0821070:intron 20.0%
!!! CTATTTTATTGTTTTGAATG+GGG + chr5_3:58857618-58857637 Msa0821070:intron 20.0%
!! ACACGAAAAATTAAAATTGA+GGG - chr5_3:58858022-58858041 None:intergenic 20.0%
!! AACACGAAAAATTAAAATTG+AGG - chr5_3:58858023-58858042 None:intergenic 20.0%
!!! CATCTAACATAATTTGAAAT+CGG + chr5_3:58858198-58858217 Msa0821070:intron 20.0%
!! GTTATTGTCAATTGTAAATA+TGG + chr5_3:58858512-58858531 Msa0821070:intron 20.0%
!!! GAGTCTTTGATTTTATTTAT+TGG + chr5_3:58858632-58858651 Msa0821070:intron 20.0%
!! AAAATTACAAAATGTCTTCT+AGG - chr5_3:58858704-58858723 None:intergenic 20.0%
!!! GAAATAAAAATAGAGTACTT+TGG - chr5_3:58858816-58858835 None:intergenic 20.0%
!! AATCATTGCATGTTTATTAT+AGG + chr5_3:58858989-58859008 Msa0821070:intron 20.0%
!! AGTATTATGATTATACAACT+AGG - chr5_3:58859082-58859101 None:intergenic 20.0%
!! AAAAATTTATAACGACTAGA+AGG - chr5_3:58859512-58859531 None:intergenic 20.0%
!!! TCATTAAGTGATATTTTTAC+AGG + chr5_3:58859663-58859682 Msa0821070:intron 20.0%
!!! AAACTTTAAATAGAAGCATA+TGG - chr5_3:58860301-58860320 None:intergenic 20.0%
!!! GATTTTTTAGTTTAAGTTCA+TGG + chr5_3:58860475-58860494 Msa0821070:three_prime_UTR 20.0%
!!! GGTTTTTCAAATTCTATGTT+GGG + chr5_3:58857431-58857450 Msa0821070:intron 25.0%
!! TTGATTCGAATTGAAAAGAT+TGG + chr5_3:58857477-58857496 Msa0821070:intron 25.0%
!!! TTTTTACTGTTTCTGAGATT+TGG + chr5_3:58857558-58857577 Msa0821070:intron 25.0%
!!! TTTTACTGTTTCTGAGATTT+GGG + chr5_3:58857559-58857578 Msa0821070:intron 25.0%
!!! TTTTTACTGTTTCTGAGATT+TGG + chr5_3:58857558-58857577 Msa0821070:intron 25.0%
!!! TTTTACTGTTTCTGAGATTT+GGG + chr5_3:58857559-58857578 Msa0821070:intron 25.0%
!! ATTTGGGTTATTGAAATTCA+GGG + chr5_3:58857668-58857687 Msa0821070:intron 25.0%
!!! CATTTTTCAGTAATATACTG+TGG + chr5_3:58858128-58858147 Msa0821070:intron 25.0%
!! CTTTCTGAAAATAAATGACT+AGG - chr5_3:58858332-58858351 None:intergenic 25.0%
!! TATTGGTCTAAGAATTAATC+TGG + chr5_3:58858649-58858668 Msa0821070:intron 25.0%
! AGAATTAATCTGGTAATTAG+TGG + chr5_3:58858659-58858678 Msa0821070:intron 25.0%
! ATAAATAATCCACACGAAAA+AGG + chr5_3:58858745-58858764 Msa0821070:intron 25.0%
!!! GGTTTTTTAGAGTAGTATTA+TGG + chr5_3:58858916-58858935 Msa0821070:intron 25.0%
! CATGTTTATTATAGGCAATT+AGG + chr5_3:58858997-58859016 Msa0821070:intron 25.0%
!! TTTTGACATGACATACTATA+AGG + chr5_3:58859111-58859130 Msa0821070:intron 25.0%
! CTACTAACTAATTTGATCAT+TGG + chr5_3:58859345-58859364 Msa0821070:intron 25.0%
! ATCATTGGAATTTATTTGTC+AGG + chr5_3:58859360-58859379 Msa0821070:intron 25.0%
!!! AAGCATATTTTTGAGAAAAG+TGG + chr5_3:58859551-58859570 Msa0821070:intron 25.0%
!!! TTAAGTGATATTTTTACAGG+TGG + chr5_3:58859666-58859685 Msa0821070:intron 25.0%
!!! GTTTTGTTACAAGAATTGTA+TGG + chr5_3:58860188-58860207 Msa0821070:three_prime_UTR 25.0%
!! AGAGAGATGGAAATATTTTA+TGG + chr5_3:58860215-58860234 Msa0821070:three_prime_UTR 25.0%
!!! TTGTGTATCGATTCAATAAA+CGG - chr5_3:58860341-58860360 None:intergenic 25.0%
! AACTTAAACTAAAAAATCGG+TGG - chr5_3:58860473-58860492 None:intergenic 25.0%
!! GTTTAAGTTCATGGTAATAA+TGG + chr5_3:58860484-58860503 Msa0821070:three_prime_UTR 25.0%
! TAGAAATTGAGAGGATTTTG+GGG - chr5_3:58857356-58857375 None:intergenic 30.0%
! GTAGAAATTGAGAGGATTTT+GGG - chr5_3:58857357-58857376 None:intergenic 30.0%
! AGTAGAAATTGAGAGGATTT+TGG - chr5_3:58857358-58857377 None:intergenic 30.0%
!! GGGTTTTTCAAATTCTATGT+TGG + chr5_3:58857430-58857449 Msa0821070:intron 30.0%
! GATTTGGGTTATTGAAATTC+AGG + chr5_3:58857667-58857686 Msa0821070:intron 30.0%
AAGGATGATATCAAAGTGTA+TGG + chr5_3:58857702-58857721 Msa0821070:CDS 30.0%
CAAAGTGTATGGATATGTTT+CGG + chr5_3:58857713-58857732 Msa0821070:CDS 30.0%
!!! TTTTGTATGATTAGTTTCCC+TGG + chr5_3:58857925-58857944 Msa0821070:intron 30.0%
! TGTATTTCTTAGAGTATCAC+TGG + chr5_3:58858102-58858121 Msa0821070:intron 30.0%
! GTATTTCTTAGAGTATCACT+GGG + chr5_3:58858103-58858122 Msa0821070:intron 30.0%
GAAAAGCTTACAGGTTTAAA+GGG - chr5_3:58858274-58858293 None:intergenic 30.0%
TGAAAAGCTTACAGGTTTAA+AGG - chr5_3:58858275-58858294 None:intergenic 30.0%
ACTTAAGATGAAAAGCTTAC+AGG - chr5_3:58858283-58858302 None:intergenic 30.0%
!! TTGTTTTTCACTTTTGAGCA+TGG - chr5_3:58858561-58858580 None:intergenic 30.0%
AGAATACAAAACACTAGAGT+TGG - chr5_3:58858596-58858615 None:intergenic 30.0%
GCTAAGTTAGTAACTATGAA+AGG + chr5_3:58859201-58859220 Msa0821070:intron 30.0%
AGATACAAGATCGTCAATAA+TGG - chr5_3:58859965-58859984 None:intergenic 30.0%
ATGGTAATAATGGTTACATG+TGG + chr5_3:58860494-58860513 Msa0821070:three_prime_UTR 30.0%
! TGATTTTCATAAATATCCCG+AGG + chr5_3:58860524-58860543 Msa0821070:three_prime_UTR 30.0%
AGAGTTGCGTGTATGATTTA+GGG + chr5_3:58857121-58857140 Msa0821070:five_prime_UTR 35.0%
!!! GTGTTTTGGCCTTCAATAAT+TGG - chr5_3:58857172-58857191 None:intergenic 35.0%
!!! TGAAAGTTGAGAGAGTGTTT+TGG - chr5_3:58857186-58857205 None:intergenic 35.0%
!! GGGTTTTTCAAATTCTATGC+TGG + chr5_3:58857528-58857547 Msa0821070:intron 35.0%
ATTCAGGGATCAATTGACAA+AGG + chr5_3:58857683-58857702 Msa0821070:intron 35.0%
TTATGTCACGTAGAGTTGTA+TGG + chr5_3:58858066-58858085 Msa0821070:intron 35.0%
TTTCAGCCAATATAGCAACA+TGG + chr5_3:58858891-58858910 Msa0821070:intron 35.0%
AACCATCCATGTTGCTATAT+TGG - chr5_3:58858900-58858919 None:intergenic 35.0%
GCAATGATTAAAAACATGCG+TGG - chr5_3:58858978-58858997 None:intergenic 35.0%
GACATGACATACTATAAGGA+TGG + chr5_3:58859115-58859134 Msa0821070:intron 35.0%
ACTTGGTAAACATCTCTATC+AGG - chr5_3:58859168-58859187 None:intergenic 35.0%
TAGCTAACAACTATGTCACT+TGG - chr5_3:58859185-58859204 None:intergenic 35.0%
CCTAAACTTCACAACAACTT+GGG - chr5_3:58859287-58859306 None:intergenic 35.0%
ACCTAAACTTCACAACAACT+TGG - chr5_3:58859288-58859307 None:intergenic 35.0%
AGTTGTATTGAAGTCACTGA+AGG + chr5_3:58859320-58859339 Msa0821070:intron 35.0%
TTACTCTCTTTCTTCTTTGC+GGG - chr5_3:58859484-58859503 None:intergenic 35.0%
CTTACTCTCTTTCTTCTTTG+CGG - chr5_3:58859485-58859504 None:intergenic 35.0%
AGAAAAGTGGTCATTGAGTT+TGG + chr5_3:58859564-58859583 Msa0821070:intron 35.0%
CAATTCCTATGTGACTGATT+TGG + chr5_3:58859870-58859889 Msa0821070:intron 35.0%
TATTGACGATCTTGTATCTG+TGG + chr5_3:58859966-58859985 Msa0821070:CDS 35.0%
!!! AGTTTTTGGCCATTTTCAAG+CGG + chr5_3:58860080-58860099 Msa0821070:three_prime_UTR 35.0%
! GCTGATTAGACTGTTATTTG+CGG + chr5_3:58860102-58860121 Msa0821070:three_prime_UTR 35.0%
GTCGTTTCTTCTTTCATGTT+TGG + chr5_3:58860150-58860169 Msa0821070:three_prime_UTR 35.0%
AAACTAAAAAATCGGTGGTG+TGG - chr5_3:58860468-58860487 None:intergenic 35.0%
AATCGTCATATTAGCGTTCT+TGG + chr5_3:58860550-58860569 Msa0821070:three_prime_UTR 35.0%
GAGAGTTGCGTGTATGATTT+AGG + chr5_3:58857120-58857139 Msa0821070:five_prime_UTR 40.0%
CATTAGGGTTTGCGATGAAT+CGG - chr5_3:58857246-58857265 None:intergenic 40.0%
TGAGAAGGTAACAGCCATTA+GGG - chr5_3:58857261-58857280 None:intergenic 40.0%
TTCTGTGTGAAGATCTGAGA+AGG - chr5_3:58857276-58857295 None:intergenic 40.0%
TCAGATCTTCACACAGAAGA+GGG + chr5_3:58857278-58857297 Msa0821070:CDS 40.0%
TCCAGAGAGGAAATCATTGA+GGG - chr5_3:58857312-58857331 None:intergenic 40.0%
TTCCAGAGAGGAAATCATTG+AGG - chr5_3:58857313-58857332 None:intergenic 40.0%
!! ACATTGGCAAAAGCATCACT+TGG - chr5_3:58857744-58857763 None:intergenic 40.0%
! GAGATACATACAGCTTTAGC+AGG - chr5_3:58858000-58858019 None:intergenic 40.0%
!! AAGTACTGTCCTTTTTCGTG+TGG - chr5_3:58858757-58858776 None:intergenic 40.0%
AGCCAATATAGCAACATGGA+TGG + chr5_3:58858895-58858914 Msa0821070:intron 40.0%
TGCGTGGATCTCATTAAGAA+AGG - chr5_3:58858962-58858981 None:intergenic 40.0%
CTATCAGGTTCAACTAACAC+AGG - chr5_3:58859153-58859172 None:intergenic 40.0%
CCCAAGTTGTTGTGAAGTTT+AGG + chr5_3:58859284-58859303 Msa0821070:intron 40.0%
GATGATGATCTTGACCTCTT+TGG + chr5_3:58859403-58859422 Msa0821070:CDS 40.0%
TGAAACAGAGGAGGATAAGA+AGG + chr5_3:58859429-58859448 Msa0821070:CDS 40.0%
TGGCTTAACATCAAGCAGAA+TGG - chr5_3:58859700-58859719 None:intergenic 40.0%
TTCTGCTTGATGTTAAGCCA+TGG + chr5_3:58859700-58859719 Msa0821070:CDS 40.0%
TCTGCTTGATGTTAAGCCAT+GGG + chr5_3:58859701-58859720 Msa0821070:CDS 40.0%
TTGAAATTCCCGGTTTAACC+TGG + chr5_3:58859769-58859788 Msa0821070:CDS 40.0%
TGAAATTCCCGGTTTAACCT+GGG + chr5_3:58859770-58859789 Msa0821070:CDS 40.0%
ACAAGCCAAATCAGTCACAT+AGG - chr5_3:58859878-58859897 None:intergenic 40.0%
CTGTGAGTCTTTCTTCAATG+AGG - chr5_3:58859996-58860015 None:intergenic 40.0%
CTCTGGACATACTCGTTAAT+TGG - chr5_3:58860024-58860043 None:intergenic 40.0%
TCTAATCAGCCGCTTGAAAA+TGG - chr5_3:58860092-58860111 None:intergenic 40.0%
CATGAAAGAAGAAACGACAG+TGG - chr5_3:58860148-58860167 None:intergenic 40.0%
!! TCATGTTTGGTGTGTTTCAG+TGG + chr5_3:58860163-58860182 Msa0821070:three_prime_UTR 40.0%
ATTGTATGGAGTGAGAGAGA+TGG + chr5_3:58860202-58860221 Msa0821070:three_prime_UTR 40.0%
ATTGGCATGGCATATGCAAA+AGG - chr5_3:58860409-58860428 None:intergenic 40.0%
CCATCACTTCTTTATTGGCA+TGG - chr5_3:58860422-58860441 None:intergenic 40.0%
CCATGCCAATAAAGAAGTGA+TGG + chr5_3:58860419-58860438 Msa0821070:three_prime_UTR 40.0%
TGCCAATAAAGAAGTGATGG+TGG + chr5_3:58860422-58860441 Msa0821070:three_prime_UTR 40.0%
GCCAATAAAGAAGTGATGGT+GGG + chr5_3:58860423-58860442 Msa0821070:three_prime_UTR 40.0%
TAATATGACGATTGTGCCTC+GGG - chr5_3:58860543-58860562 None:intergenic 40.0%
CTAATATGACGATTGTGCCT+CGG - chr5_3:58860544-58860563 None:intergenic 40.0%
CGATTCATCGCAAACCCTAA+TGG + chr5_3:58857244-58857263 Msa0821070:exon 45.0%
CTGAGAAGGTAACAGCCATT+AGG - chr5_3:58857262-58857281 None:intergenic 45.0%
CTCAGATCTTCACACAGAAG+AGG + chr5_3:58857277-58857296 Msa0821070:CDS 45.0%
TCCCTCAATGATTTCCTCTC+TGG + chr5_3:58857308-58857327 Msa0821070:CDS 45.0%
! GATGCTTTTGCCAATGTTGC+TGG + chr5_3:58857747-58857766 Msa0821070:CDS 45.0%
! CTTTTGCCAATGTTGCTGGT+TGG + chr5_3:58857751-58857770 Msa0821070:CDS 45.0%
ATCGTACCAACCAGCAACAT+TGG - chr5_3:58857760-58857779 None:intergenic 45.0%
TCTCTTCACATCTTGCTGCA+AGG + chr5_3:58857784-58857803 Msa0821070:CDS 45.0%
AGTTTCCCTGGAAAAGCTCA+AGG + chr5_3:58857937-58857956 Msa0821070:intron 45.0%
! TCACACCTTGAGCTTTTCCA+GGG - chr5_3:58857945-58857964 None:intergenic 45.0%
! TTCACACCTTGAGCTTTTCC+AGG - chr5_3:58857946-58857965 None:intergenic 45.0%
GCTCAAGGTGTGAAATTCAG+TGG + chr5_3:58857952-58857971 Msa0821070:CDS 45.0%
AGAAAGGAGCTCTACAGTAC+TGG - chr5_3:58858946-58858965 None:intergenic 45.0%
TCATCATCATCTTCAGCAGC+AGG - chr5_3:58859391-58859410 None:intergenic 45.0%
CCTCTGTTTCATCACCAAAG+AGG - chr5_3:58859420-58859439 None:intergenic 45.0%
!! CCTCTTTGGTGATGAAACAG+AGG + chr5_3:58859417-58859436 Msa0821070:CDS 45.0%
!! CTTTGGTGATGAAACAGAGG+AGG + chr5_3:58859420-58859439 Msa0821070:CDS 45.0%
ATAAGAAGGCAGCAGAGGAA+AGG + chr5_3:58859443-58859462 Msa0821070:CDS 45.0%
TAAGAAGGCAGCAGAGGAAA+GGG + chr5_3:58859444-58859463 Msa0821070:CDS 45.0%
ATGTCTGTCTCATCATCCCA+TGG - chr5_3:58859720-58859739 None:intergenic 45.0%
TGAGACAGACATGAAGAAGC+TGG + chr5_3:58859728-58859747 Msa0821070:CDS 45.0%
AGACATGAAGAAGCTGGAAG+AGG + chr5_3:58859734-58859753 Msa0821070:CDS 45.0%
GAAATTCCCGGTTTAACCTG+GGG + chr5_3:58859771-58859790 Msa0821070:CDS 45.0%
TTTCAACATACATGCTCCCC+AGG - chr5_3:58859790-58859809 None:intergenic 45.0%
TTGCATTGTTACAGCCAAGC+TGG + chr5_3:58859897-58859916 Msa0821070:intron 45.0%
! AAGCTGGTTGCTGTTGGATA+CGG + chr5_3:58859913-58859932 Msa0821070:CDS 45.0%
GAAAGAAGAAACGACAGTGG+AGG - chr5_3:58860145-58860164 None:intergenic 45.0%
!! TGTTTGGTGTGTTTCAGTGG+TGG + chr5_3:58860166-58860185 Msa0821070:three_prime_UTR 45.0%
TCAAACGCCATGCACATTTG+TGG + chr5_3:58860259-58860278 Msa0821070:three_prime_UTR 45.0%
CAAACGCCATGCACATTTGT+GGG + chr5_3:58860260-58860279 Msa0821070:three_prime_UTR 45.0%
GCTAATCCCACAAATGTGCA+TGG - chr5_3:58860269-58860288 None:intergenic 45.0%
GCCCACCATCACTTCTTTAT+TGG - chr5_3:58860427-58860446 None:intergenic 45.0%
!!! TTGATTTTTTTTTTTATAAA+AGG - chr5_3:58859853-58859872 None:intergenic 5.0%
TAGGGTTTGCGATGAATCGG+TGG - chr5_3:58857243-58857262 None:intergenic 50.0%
! GTTCGCAGCGTTGAAATTCC+CGG + chr5_3:58859759-58859778 Msa0821070:CDS 50.0%
ACATGCTCCCCAGGTTAAAC+CGG - chr5_3:58859781-58859800 None:intergenic 50.0%
GTATCCAACAGCAACCAGCT+TGG - chr5_3:58859914-58859933 None:intergenic 50.0%
ATGCGACAATGTCACAGCTC+TGG - chr5_3:58860041-58860060 None:intergenic 50.0%
GAAACGACAGTGGAGGAGAA+GGG - chr5_3:58860138-58860157 None:intergenic 50.0%
AGAAACGACAGTGGAGGAGA+AGG - chr5_3:58860139-58860158 None:intergenic 50.0%
GGTTTGCGATGAATCGGTGG+CGG - chr5_3:58857240-58857259 None:intergenic 55.0%
GGAGGATAAGAAGGCAGCAG+AGG + chr5_3:58859438-58859457 Msa0821070:CDS 55.0%
CATGCTCCCCAGGTTAAACC+GGG - chr5_3:58859780-58859799 None:intergenic 55.0%
ACAGCCAAGCTGGTTGCTGT+TGG + chr5_3:58859907-58859926 Msa0821070:intron 55.0%
!! AGTGGAAGTGCTCCAGCTGC+AGG + chr5_3:58857970-58857989 Msa0821070:CDS 60.0%
TTAGCAGGTGCTCCTGCAGC+TGG - chr5_3:58857985-58858004 None:intergenic 60.0%
GAAGGCAGCAGAGGAAAGGG+AGG + chr5_3:58859447-58859466 Msa0821070:CDS 60.0%
ACGACAGTGGAGGAGAAGGG+AGG - chr5_3:58860135-58860154 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 58857086 58860589 58857086 ID=Msa0821070;Name=Msa0821070
chr5_3 mRNA 58857086 58860589 58857086 ID=Msa0821070-mRNA-1;Parent=Msa0821070;Name=Msa0821070-mRNA-1;_AED=0.01;_eAED=0.01;_QI=177|1|1|1|1|1|6|515|222
chr5_3 exon 58857086 58857348 58857086 ID=Msa0821070-mRNA-1:exon:17892;Parent=Msa0821070-mRNA-1
chr5_3 exon 58857689 58857805 58857689 ID=Msa0821070-mRNA-1:exon:17893;Parent=Msa0821070-mRNA-1
chr5_3 exon 58857939 58858008 58857939 ID=Msa0821070-mRNA-1:exon:17894;Parent=Msa0821070-mRNA-1
chr5_3 exon 58859382 58859499 58859382 ID=Msa0821070-mRNA-1:exon:17895;Parent=Msa0821070-mRNA-1
chr5_3 exon 58859685 58859798 58859685 ID=Msa0821070-mRNA-1:exon:17896;Parent=Msa0821070-mRNA-1
chr5_3 exon 58859911 58860589 58859911 ID=Msa0821070-mRNA-1:exon:17897;Parent=Msa0821070-mRNA-1
chr5_3 five_prime_UTR 58857086 58857262 58857086 ID=Msa0821070-mRNA-1:five_prime_utr;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58857263 58857348 58857263 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58857689 58857805 58857689 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58857939 58858008 58857939 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58859382 58859499 58859382 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58859685 58859798 58859685 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 CDS 58859911 58860074 58859911 ID=Msa0821070-mRNA-1:cds;Parent=Msa0821070-mRNA-1
chr5_3 three_prime_UTR 58860075 58860589 58860075 ID=Msa0821070-mRNA-1:three_prime_utr;Parent=Msa0821070-mRNA-1
Gene Sequence

>Msa0821070

ATGGCTGTTACCTTCTCAGATCTTCACACAGAAGAGGGTCTCAAATCCCTCAATGATTTCCTCTCTGGAAAAAAATATATTTCTGGGGATCAATTGACAAAGGATGATATCAAAGTGTATGGATATGTTTCGGAGAAACCAAGTGATGCTTTTGCCAATGTTGCTGGTTGGTACGATGTTGTCTCTTCACATCTTGCTGCAAGTTTCCCTGGAAAAGCTCAAGGTGTGAAATTCAGTGGAAGTGCTCCAGCTGCAGGAGCACCTGCTAAAGCTGCTGCACCTGCTGCTGAAGATGATGATGATCTTGACCTCTTTGGTGATGAAACAGAGGAGGATAAGAAGGCAGCAGAGGAAAGGGAGGCATCTAAAAAGCCCGCAAAGAAGAAAGAGAGTGGCAAGTCTTCCATTCTGCTTGATGTTAAGCCATGGGATGATGAGACAGACATGAAGAAGCTGGAAGAGGCTGTTCGCAGCGTTGAAATTCCCGGTTTAACCTGGGGAGCATCCAAGCTGGTTGCTGTTGGATACGGAATCAAGAAGCTGCAGATCATGTTGACCATTATTGACGATCTTGTATCTGTGGACGACCTCATTGAAGAAAGACTCACAGTTGAGCCAATTAACGAGTATGTCCAGAGCTGTGACATTGTCGCATTCAACAAAATTTAA
Protein sequence

>Msa0821070

MAVTFSDLHTEEGLKSLNDFLSGKKYISGDQLTKDDIKVYGYVSEKPSDAFANVAGWYDVVSSHLAASFPGKAQGVKFSGSAPAAGAPAKAAAPAAEDDDDLDLFGDETEEDKKAAEEREASKKPAKKKESGKSSILLDVKPWDDETDMKKLEEAVRSVEIPGLTWGASKLVAVGYGIKKLQIMLTIIDDLVSVDDLIEERLTVEPINEYVQSCDIVAFNKI*