AlfalfaGEDB | Msa0830270

M. sativa cultivar zhongmu-4 / Msa0830270

KEGG: K00913
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006793 GO:0006796 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009611 GO:0009791 GO:0009987 GO:0010154 GO:0010214 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044464 GO:0047325 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051765 GO:0051766 GO:0052725 GO:0052726 GO:0061458
PFAM: PF17927.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830270	AES93601.1	90.407	344	6	2	1	344	1	317	0.0	615

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830270	sp\|Q9SUG3\|ITPK3_ARATH	68.060	335	82	2	13	347	38	347	6.43e-155	442

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830270	G7K0Z3	90.407	344	6	2	1	344	1	317	0.0	615

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0101810	Msa0830270	-0.805928	1.053457e-49	-8.615850e-47
Msa0190830	Msa0830270	0.816477	5.420004e-52	-8.615850e-47
Msa0272480	Msa0830270	-0.816973	4.194964e-52	-8.615850e-47
Msa0311580	Msa0830270	0.810258	1.260632e-50	-8.615850e-47
Msa0312690	Msa0830270	-0.821544	3.816730e-53	-8.615850e-47
Msa0620420	Msa0830270	0.920744	8.680031e-88	-8.615850e-47
Msa0711180	Msa0830270	0.821563	3.778565e-53	-8.615850e-47
Msa0756630	Msa0830270	-0.802846	4.621992e-49	-8.615850e-47
Msa0802470	Msa0830270	-0.819247	1.284146e-52	-8.615850e-47
Msa0830270	Msa0981820	-0.810720	1.001762e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830270	MtrunA17_Chr5g0393881	90.407	344	6	2	1	344	1	317	0.0	613
Msa0830270	MtrunA17_Chr1g0206731	74.251	334	63	1	14	347	29	339	5.66e-173	484
Msa0830270	MtrunA17_Chr8g0366491	39.377	353	169	8	12	349	3	325	1.09e-79	248
Msa0830270	MtrunA17_Chr4g0059021	37.082	329	177	4	18	341	10	313	5.36e-70	221
Msa0830270	MtrunA17_Chr3g0111511	26.349	315	187	11	16	298	161	462	9.33e-19	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830270	AT4G08170.2	68.060	335	82	2	13	347	38	347	6.54e-156	442
Msa0830270	AT4G33770.1	62.462	333	100	2	15	347	80	387	9.16e-139	399
Msa0830270	AT4G33770.2	60.377	318	97	3	30	347	6	294	1.31e-126	365
Msa0830270	AT4G08170.1	67.986	278	64	2	70	347	7	259	1.97e-125	361
Msa0830270	AT4G08170.3	67.901	243	53	2	105	347	1	218	5.20e-105	307
Msa0830270	AT4G08170.6	67.901	243	53	2	105	347	1	218	5.20e-105	307
Msa0830270	AT4G08170.5	67.901	243	53	2	105	347	1	218	5.20e-105	307
Msa0830270	AT4G08170.7	67.901	243	53	2	105	347	1	218	5.20e-105	307
Msa0830270	AT4G08170.4	67.901	243	53	2	105	347	1	218	5.20e-105	307
Msa0830270	AT4G33770.3	58.025	243	77	2	105	347	1	218	3.90e-86	259

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Find 95 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGCCGATGTTAGAACA+AGG	0.679946	5_4:+3304084	Msa0830270:CDS
AACGGACCTTGTTCTAACAT+CGG	0.598617	5_4:-3304090	None:intergenic
AACTCTTGAAGAACAAGTGG+TGG	0.581060	5_4:-3306520	None:intergenic
AAGAAACCACTGCTAAGCCA+AGG	0.617574	5_4:-3307496	None:intergenic
AAGGGACCTCCGTCGTAGAC+TGG	0.506046	5_4:+3307006	Msa0830270:CDS
AAGTGCTGAATTGCATCTGG+AGG	0.621643	5_4:-3305859	None:intergenic
AATAAGTGTAAGAAGAAGGA+TGG	0.508168	5_4:+3307426	Msa0830270:CDS
AATACACAAAATAGAATCTC+TGG	0.413683	5_4:+3303169	Msa0830270:five_prime_UTR
AATCAGTAACATGTTAATTC+CGG	0.360602	5_4:+3307522	Msa0830270:three_prime_UTR
AATGAATGAATACCTTGCAA+GGG	0.624486	5_4:-3303334	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCTATAAACTGAAACAACT+TGG	+	chr5_4:3303140-3303159	Msa0830270:five_prime_UTR	30.0%
TATAAACTGAAACAACTTGG+AGG	+	chr5_4:3303143-3303162	Msa0830270:five_prime_UTR	30.0%
GGTAAGAAAATGAAGTTGAA+CGG	+	chr5_4:3303216-3303235	Msa0830270:exon	30.0%
AATGAATGAATACCTTGCAA+GGG	-	chr5_4:3303337-3303356	None:intergenic	30.0%
ACCTTGAATTATCAAGCAAA+GGG	-	chr5_4:3303606-3303625	None:intergenic	30.0%
AAAGGAAAGAGGAAAATGTA+TGG	-	chr5_4:3303728-3303747	None:intergenic	30.0%
AAAACATCCAAAAAGGAAAG+AGG	-	chr5_4:3303739-3303758	None:intergenic	30.0%
AATCCAGAAAACATCCAAAA+AGG	-	chr5_4:3303746-3303765	None:intergenic	30.0%
ATTTGGTCCTTGCAAAATAT+AGG	+	chr5_4:3303826-3303845	Msa0830270:intron	30.0%
TTTGGTCCTTGCAAAATATA+GGG	+	chr5_4:3303827-3303846	Msa0830270:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	3302980	3307723	3302980	ID=Msa0830270;Name=Msa0830270
chr5_4	mRNA	3302980	3307723	3302980	ID=Msa0830270-mRNA-1;Parent=Msa0830270;Name=Msa0830270-mRNA-1;_AED=0.15;_eAED=0.16;_QI=245\|0.88\|0.9\|1\|1\|1\|10\|268\|350
chr5_4	exon	3302980	3303343	3302980	ID=Msa0830270-mRNA-1:exon:1223;Parent=Msa0830270-mRNA-1
chr5_4	exon	3304050	3304131	3304050	ID=Msa0830270-mRNA-1:exon:1224;Parent=Msa0830270-mRNA-1
chr5_4	exon	3304257	3304289	3304257	ID=Msa0830270-mRNA-1:exon:1225;Parent=Msa0830270-mRNA-1
chr5_4	exon	3305820	3305940	3305820	ID=Msa0830270-mRNA-1:exon:1226;Parent=Msa0830270-mRNA-1
chr5_4	exon	3306036	3306134	3306036	ID=Msa0830270-mRNA-1:exon:1227;Parent=Msa0830270-mRNA-1
chr5_4	exon	3306434	3306553	3306434	ID=Msa0830270-mRNA-1:exon:1228;Parent=Msa0830270-mRNA-1
chr5_4	exon	3306677	3306853	3306677	ID=Msa0830270-mRNA-1:exon:1229;Parent=Msa0830270-mRNA-1
chr5_4	exon	3306972	3307027	3306972	ID=Msa0830270-mRNA-1:exon:1230;Parent=Msa0830270-mRNA-1
chr5_4	exon	3307111	3307264	3307111	ID=Msa0830270-mRNA-1:exon:1231;Parent=Msa0830270-mRNA-1
chr5_4	exon	3307364	3307723	3307364	ID=Msa0830270-mRNA-1:exon:1232;Parent=Msa0830270-mRNA-1
chr5_4	five_prime_UTR	3302980	3303224	3302980	ID=Msa0830270-mRNA-1:five_prime_utr;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3303225	3303343	3303225	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3304050	3304131	3304050	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3304257	3304289	3304257	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3305820	3305940	3305820	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3306036	3306134	3306036	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3306434	3306553	3306434	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3306677	3306853	3306677	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3306972	3307027	3306972	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3307111	3307264	3307111	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	CDS	3307364	3307455	3307364	ID=Msa0830270-mRNA-1:cds;Parent=Msa0830270-mRNA-1
chr5_4	three_prime_UTR	3307456	3307723	3307456	ID=Msa0830270-mRNA-1:three_prime_utr;Parent=Msa0830270-mRNA-1

Gene Sequence

>Msa0830270

ATGAAGTTGAACGGTGAAGAAGAAGAAGAAGAGGAGGAGGAGAAAGTGGTTGTTGGATACGCCTTAACATCAAAGAAGAAGAAAAGCTTTCTAAAACCAAACTTTATTGCCCTTGCAAGGAATAAGGGGATACTTTTTGTTGCTGTTGATCTAAACAAGCCGATGTTAGAACAAGGTCCGTTTGATGTTGTCTTGCATAAGTTGCCAGGAAAAGAGTGGTGTGAGATTATCGAGGATTACAGGCATAAACATCCCGAAGTAACCATCCTTGACCCTCCAGATGCAATTCAGCACTTGCTCAATCGACAATCGATGCTACAAAATGTGGCAGAGCTAAACTTATCTGACTGCCATGGGAAGGTTGGTGTTCCACAACAGTTGGTTATCACTAAAAACGCATCTGCATCTACTATCCCCTATGAAGTCACTAAGGCTGGAATGAAGTTACCGTTAGTTGCAAAACCATTAGTTGTGGATGGGAGTGCGAAGTCGCATGAATTGTGCATTGCTTATGATGAACTGTCACTTTTGAAGCTTGAGCCACCACTTGTTCTTCAAGAGTTTGTCAATCATGGTGGTCTTCTCTTTAAGATTTATATTGTTGGCGAAACTATAAAGGTTGTGAGGCGTTTCTCTCTACCTAATGTCGGCAAACATGAATTGCTGGGTGTTGATGGTTTATTCCGATTTCCAAGAGTTTCATGTGCAGCAGCTTCTGCAGATGAAGCTGATTTGGATCCAAATATTGCGGAACATCCACCAAAACCTTTATTGGAGAGGCTTGCAAGGGACCTCCGTCGTAGACTGGGACTCCACTTGTTCAACATTGATATGATACGGGAACATGGGACAAAGGATGTGTTTTATGTCATTGATATCAATTACTTTCCAGGCAAGTACAACTTATGTCAATTTATGTCATTGATTATTTTTTGTGTATCAAAGTTAAAGTTTCTTTGTAGGTATGGAAAAATGCCAGAGTATGAACACATATTTATAGATTTCCTACTTAGCCTTGTGAAGAATAAGTGTAAGAAGAAGGATGGTACTTAA

Protein sequence

>Msa0830270

MKLNGEEEEEEEEEKVVVGYALTSKKKKSFLKPNFIALARNKGILFVAVDLNKPMLEQGPFDVVLHKLPGKEWCEIIEDYRHKHPEVTILDPPDAIQHLLNRQSMLQNVAELNLSDCHGKVGVPQQLVITKNASASTIPYEVTKAGMKLPLVAKPLVVDGSAKSHELCIAYDELSLLKLEPPLVLQEFVNHGGLLFKIYIVGETIKVVRRFSLPNVGKHELLGVDGLFRFPRVSCAAASADEADLDPNIAEHPPKPLLERLARDLRRRLGLHLFNIDMIREHGTKDVFYVIDINYFPGKYNLCQFMSLIIFCVSKLKFLCRYGKMPEYEHIFIDFLLSLVKNKCKKKDGT*