AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0611540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611540 MtrunA17_Chr4g0044951 80.000 110 13 3 1 105 1 106 8.01e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0611540 AT3G19220.1 41.584 101 54 2 10 105 16 116 2.14e-15 68.6

Find 76 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTTCCAGTCATTTCTT+TGG 0.224977 4_2:-59760098 None:intergenic
TCATTTCCAGTCATTTCTTT+GGG 0.242299 4_2:-59760097 None:intergenic
TCGGAAAGATATTGATTTAA+TGG 0.291501 4_2:+59759840 Msa0611540:three_prime_UTR
CTAAAGAAAAGCAGAATTAA+TGG 0.315641 4_2:-59760181 None:intergenic
AAAATCCCAAGAAGGAAAAT+AGG 0.317287 4_2:+59760120 Msa0611540:three_prime_UTR
AGGCGTTCATCAACGACTTC+AGG 0.372761 4_2:-59756833 None:intergenic
TAAAGAAAAGCAGAATTAAT+GGG 0.372907 4_2:-59760180 None:intergenic
TTCTACGACGGCCGAGGTGG+TGG 0.373631 4_2:-59756657 None:intergenic
ACGAAATAGAGAAGGATATA+TGG 0.381212 4_2:+59756751 Msa0611540:CDS
GGAGAAACAGGAGAAGAATC+AGG 0.384713 4_2:-59756812 None:intergenic
GGGCCGTGGGGAAAGGTCTC+CGG 0.391665 4_2:-59756597 None:intergenic
CCGCTGCTAGACGCGCCGGC+GGG 0.397750 4_2:+59756687 Msa0611540:CDS
CGAGGGATAGAGGTGGGCCG+TGG 0.399101 4_2:-59756611 None:intergenic
GGAAAGCATACAGAGATCTA+AGG 0.407306 4_2:+59760141 Msa0611540:three_prime_UTR
GAAGGATATATGGTTGATAA+TGG 0.408638 4_2:+59756761 Msa0611540:CDS
ACCACCTCGGCCGTCGTAGA+AGG 0.411149 4_2:+59756659 Msa0611540:CDS
TGAAAGCTAAGGAGAGAAAA+TGG 0.412578 4_2:+59756726 Msa0611540:CDS
TATTGATTTAATGGAGGACT+TGG 0.415117 4_2:+59759849 Msa0611540:three_prime_UTR
TGGTGGGGAGGGAGAGCGAC+TGG 0.425439 4_2:-59756640 None:intergenic
AAGATGAATACAGTACAGTA+TGG 0.429640 4_2:-59759970 None:intergenic
TTAATGGAGGACTTGGGTAA+TGG 0.431654 4_2:+59759856 Msa0611540:three_prime_UTR
CTAGTTCCCTTTCTTTACCT+CGG 0.445504 4_2:+59759943 Msa0611540:three_prime_UTR
TCAACGACTTCAGGAGAAAC+AGG 0.446064 4_2:-59756824 None:intergenic
CCCGCCGGCGCGTCTAGCAG+CGG 0.458625 4_2:-59756687 None:intergenic
CTGGAAGAGACGAGGGATAG+AGG 0.462107 4_2:-59756621 None:intergenic
TAGAGGTGGGCCGTGGGGAA+AGG 0.462906 4_2:-59756604 None:intergenic
TTCCGCTACCGTGCTTCTGC+CGG 0.465937 4_2:+59756578 Msa0611540:CDS
CAAGTTTGGGGTCACATTGA+AGG 0.467284 4_2:-59756853 None:intergenic
TAGTTCCCTTTCTTTACCTC+GGG 0.469113 4_2:+59759944 Msa0611540:three_prime_UTR
TTCCTCCCCAAAGAAATGAC+TGG 0.474961 4_2:+59760091 Msa0611540:three_prime_UTR
GCCGCTGCTAGACGCGCCGG+CGG 0.485119 4_2:+59756686 Msa0611540:CDS
TCTACGACGGCCGAGGTGGT+GGG 0.486217 4_2:-59756656 None:intergenic
TCAGGGTAAGTCATGGGAAG+TGG 0.505112 4_2:-59756794 None:intergenic
CTACGACGGCCGAGGTGGTG+GGG 0.506106 4_2:-59756655 None:intergenic
GACGGCCGAGGTGGTGGGGA+GGG 0.506391 4_2:-59756651 None:intergenic
CGACGGCCGAGGTGGTGGGG+AGG 0.506589 4_2:-59756652 None:intergenic
TGGATTCCCGAGGTAAAGAA+AGG 0.507068 4_2:-59759950 None:intergenic
TAAGTCATGGGAAGTGGAAG+AGG 0.508061 4_2:-59756788 None:intergenic
GGCGCCGCTGCTAGACGCGC+CGG 0.508225 4_2:+59756683 Msa0611540:CDS
TTCCAGTCATTTCTTTGGGG+AGG 0.514828 4_2:-59760093 None:intergenic
GAGGGATAGAGGTGGGCCGT+GGG 0.518062 4_2:-59756610 None:intergenic
CTCCGGCAGAAGCACGGTAG+CGG 0.523548 4_2:-59756580 None:intergenic
AGCGGCGCCACCTTCTACGA+CGG 0.531670 4_2:-59756669 None:intergenic
GACGCGCCGGCGGGAACGAG+TGG 0.537435 4_2:+59756696 Msa0611540:CDS
CGGCGGGAACGAGTGGATGA+AGG 0.547338 4_2:+59756703 Msa0611540:CDS
GAAGAATCAGGGTAAGTCAT+GGG 0.548546 4_2:-59756800 None:intergenic
ATTGATTTAATGGAGGACTT+GGG 0.552134 4_2:+59759850 Msa0611540:three_prime_UTR
TTCTTTGGGGAGGAAAGAAG+TGG 0.559977 4_2:-59760083 None:intergenic
GTAATGGAAAACCGTTATGA+TGG 0.564931 4_2:+59759872 Msa0611540:three_prime_UTR
GAGTTATTGAGTAACAATCA+TGG 0.565921 4_2:-59760009 None:intergenic
AAGCACGGTAGCGGAAGATG+AGG 0.572632 4_2:-59756571 None:intergenic
CATTTCCAGTCATTTCTTTG+GGG 0.573023 4_2:-59760096 None:intergenic
CTTTGGGGAGGAAAGAAGTG+GGG 0.575179 4_2:-59760081 None:intergenic
GCCACCTTCTACGACGGCCG+AGG 0.576103 4_2:-59756663 None:intergenic
CTTCATCCACTCGTTCCCGC+CGG 0.576282 4_2:-59756702 None:intergenic
ACCTTCTACGACGGCCGAGG+TGG 0.577117 4_2:-59756660 None:intergenic
TCTTTGGGGAGGAAAGAAGT+GGG 0.578141 4_2:-59760082 None:intergenic
GTGGATGAAGGTGAAAGCTA+AGG 0.585939 4_2:+59756715 Msa0611540:CDS
GGATTCCCGAGGTAAAGAAA+GGG 0.590840 4_2:-59759949 None:intergenic
ACCTCGGCCGTCGTAGAAGG+TGG 0.591927 4_2:+59756662 Msa0611540:CDS
AGAAGAATCAGGGTAAGTCA+TGG 0.594348 4_2:-59756801 None:intergenic
AAATGGTCACGAAATAGAGA+AGG 0.599995 4_2:+59756743 Msa0611540:CDS
GAGAAACAGGAGAAGAATCA+GGG 0.602861 4_2:-59756811 None:intergenic
AAAGGTCTCCGGCAGAAGCA+CGG 0.606022 4_2:-59756586 None:intergenic
CTGCCGGAGACCTTTCCCCA+CGG 0.611563 4_2:+59756594 Msa0611540:CDS
GAAAGATATTGATTTAATGG+AGG 0.617023 4_2:+59759843 Msa0611540:three_prime_UTR
AGGGATAGAGGTGGGCCGTG+GGG 0.627374 4_2:-59756609 None:intergenic
GAGAACATCAACCATCATAA+CGG 0.631195 4_2:-59759883 None:intergenic
AAGAGACGAGGGATAGAGGT+GGG 0.633620 4_2:-59756617 None:intergenic
GAGAGCGACTGGAAGAGACG+AGG 0.635228 4_2:-59756629 None:intergenic
AGAGCGACTGGAAGAGACGA+GGG 0.639552 4_2:-59756628 None:intergenic
GAAGAGACGAGGGATAGAGG+TGG 0.643962 4_2:-59756618 None:intergenic
AGCACGGTAGCGGAAGATGA+GGG 0.649637 4_2:-59756570 None:intergenic
GCTCTCCCTCCCCACCACCT+CGG 0.664061 4_2:+59756646 Msa0611540:CDS
ATGTGCAGAAGATAACAGCT+CGG 0.677955 4_2:+59759821 Msa0611540:three_prime_UTR
CAGTACAGTATGGATTCCCG+AGG 0.712945 4_2:-59759960 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAATTTTTATTTTACCT+AGG + chr4_2:59759346-59759365 Msa0611540:intron 10.0%
!! AAAATTAAATAAAAAAACTG+GGG - chr4_2:59759921-59759940 None:intergenic 10.0%
!!! TATAATGAATCATAATTTGT+TGG - chr4_2:59757458-59757477 None:intergenic 15.0%
!! TTATGATTTATGATTTACTT+TGG + chr4_2:59758400-59758419 Msa0611540:intron 15.0%
!! CATTTATGAAAAAAAAGAAA+CGG - chr4_2:59758680-59758699 None:intergenic 15.0%
!! AGAATCTTATTAATTCAAAT+TGG - chr4_2:59759203-59759222 None:intergenic 15.0%
!! AAATTAAATAAAAAAACTGG+GGG - chr4_2:59759920-59759939 None:intergenic 15.0%
!! ACTATATCAAATGTCTTTAA+AGG + chr4_2:59757539-59757558 Msa0611540:intron 20.0%
!! TTTCATAATTTCTAATTAGC+AGG + chr4_2:59757703-59757722 Msa0611540:intron 20.0%
!! TTCATAATTTCTAATTAGCA+GGG + chr4_2:59757704-59757723 Msa0611540:intron 20.0%
!!! TTGTTTTTTTAGACAACAAA+AGG - chr4_2:59757813-59757832 None:intergenic 20.0%
!! AAAAAAATTGAGATGAGTAT+AGG - chr4_2:59757946-59757965 None:intergenic 20.0%
!! CTTGCATATAAAAAAACTTA+GGG - chr4_2:59758141-59758160 None:intergenic 20.0%
!! TCTTGCATATAAAAAAACTT+AGG - chr4_2:59758142-59758161 None:intergenic 20.0%
!! ATTATCATGCTAATATTCAT+AGG - chr4_2:59758368-59758387 None:intergenic 20.0%
!!! TTACTTTGGCATATATTTTA+TGG + chr4_2:59758414-59758433 Msa0611540:intron 20.0%
!!! CGTTTCTTTTTTTTCATAAA+TGG + chr4_2:59758678-59758697 Msa0611540:intron 20.0%
!!! TGTTTTTATTGACTTGTTTT+GGG - chr4_2:59758817-59758836 None:intergenic 20.0%
!!! TTGTTTTTATTGACTTGTTT+TGG - chr4_2:59758818-59758837 None:intergenic 20.0%
!! TTAAAGCTAGATATATTCTA+CGG + chr4_2:59759058-59759077 Msa0611540:intron 20.0%
!! TAAAGAAAAGCAGAATTAAT+GGG - chr4_2:59760183-59760202 None:intergenic 20.0%
! GATATAAGCTATAAGTTGTT+TGG - chr4_2:59756933-59756952 None:intergenic 25.0%
!!! TTGAGTAGATATTTTGATGA+TGG + chr4_2:59757089-59757108 Msa0611540:intron 25.0%
!!! TGAGTAGATATTTTGATGAT+GGG + chr4_2:59757090-59757109 Msa0611540:intron 25.0%
!!! ATATTTTGATGATGGGTTAT+AGG + chr4_2:59757097-59757116 Msa0611540:intron 25.0%
! TAAGCTACTATAACAAAAGA+GGG + chr4_2:59757183-59757202 Msa0611540:intron 25.0%
! ATGTAATGTAATACGTTGAT+AGG - chr4_2:59757260-59757279 None:intergenic 25.0%
! AACATATATATGATCACACT+TGG + chr4_2:59757568-59757587 Msa0611540:intron 25.0%
!!! TCATTTTTACACAACAACTA+TGG + chr4_2:59757778-59757797 Msa0611540:intron 25.0%
! CTAAAAAAACAATTTCCCTT+TGG + chr4_2:59757821-59757840 Msa0611540:intron 25.0%
!! AGTACTTGTATCTTTTACAA+AGG - chr4_2:59757847-59757866 None:intergenic 25.0%
!!! ATGCAGTTTTGTAATTTGTA+TGG - chr4_2:59757997-59758016 None:intergenic 25.0%
! TCTATGTTGAAATGGTTAAA+AGG - chr4_2:59758520-59758539 None:intergenic 25.0%
! TGTCCATTATATTTCAAACA+GGG - chr4_2:59758751-59758770 None:intergenic 25.0%
! TTGTCCATTATATTTCAAAC+AGG - chr4_2:59758752-59758771 None:intergenic 25.0%
! ACTCTGATATTTCCATTATA+AGG + chr4_2:59759023-59759042 Msa0611540:intron 25.0%
! TATTTCCATTATAAGGTTTC+AGG + chr4_2:59759030-59759049 Msa0611540:intron 25.0%
! TATAACCTGAAACCTTATAA+TGG - chr4_2:59759038-59759057 None:intergenic 25.0%
! TAAAGCTAGATATATTCTAC+GGG + chr4_2:59759059-59759078 Msa0611540:intron 25.0%
! TATTAAAGTAAGCAAGATGT+AGG + chr4_2:59759300-59759319 Msa0611540:intron 25.0%
! AATATACACATGAAATTCCT+AGG - chr4_2:59759366-59759385 None:intergenic 25.0%
! AGGAATTTCATGTGTATATT+AGG + chr4_2:59759366-59759385 Msa0611540:intron 25.0%
! ACATAGAATTAAGATCATAG+AGG - chr4_2:59759697-59759716 None:intergenic 25.0%
! AAGTGAATACAAAGAAACAA+TGG - chr4_2:59759735-59759754 None:intergenic 25.0%
!!! TTCTTTGTATTCACTTTTAC+TGG + chr4_2:59759739-59759758 Msa0611540:intron 25.0%
!!! TCGGAAAGATATTGATTTAA+TGG + chr4_2:59759840-59759859 Msa0611540:three_prime_UTR 25.0%
!!! GAAAGATATTGATTTAATGG+AGG + chr4_2:59759843-59759862 Msa0611540:three_prime_UTR 25.0%
! CTAAAGAAAAGCAGAATTAA+TGG - chr4_2:59760184-59760203 None:intergenic 25.0%
ACGAAATAGAGAAGGATATA+TGG + chr4_2:59756751-59756770 Msa0611540:CDS 30.0%
!! GAAGGATATATGGTTGATAA+TGG + chr4_2:59756761-59756780 Msa0611540:CDS 30.0%
GTTATAGTAGCTTACATATC+CGG - chr4_2:59757177-59757196 None:intergenic 30.0%
GTAAGCTACTATAACAAAAG+AGG + chr4_2:59757182-59757201 Msa0611540:intron 30.0%
! AAAAGTATACCAGCACAAAA+AGG - chr4_2:59757299-59757318 None:intergenic 30.0%
!! TTTTTAGTCAAGTAGCTTAG+TGG + chr4_2:59757345-59757364 Msa0611540:intron 30.0%
!! GAATCATAATTTGTTGGTAG+CGG - chr4_2:59757452-59757471 None:intergenic 30.0%
!!! TGCTCTCAAATTATTTTGAC+TGG + chr4_2:59757732-59757751 Msa0611540:intron 30.0%
GTAAAAATGAAAACCCTTCA+TGG - chr4_2:59757768-59757787 None:intergenic 30.0%
! TATCTTTTACAAAGGCCAAA+GGG - chr4_2:59757839-59757858 None:intergenic 30.0%
! ATGGACTGAAAACTGAATAT+AGG - chr4_2:59757978-59757997 None:intergenic 30.0%
! CATGCTTTTAGTCATTCTTT+AGG + chr4_2:59758014-59758033 Msa0611540:intron 30.0%
! ATGCAGTTTTGCAATTTGTA+GGG + chr4_2:59758047-59758066 Msa0611540:intron 30.0%
CACTATCTTCTATGTTGAAA+TGG - chr4_2:59758528-59758547 None:intergenic 30.0%
CATTTCAACATAGAAGATAG+TGG + chr4_2:59758526-59758545 Msa0611540:intron 30.0%
GATACTAAAACTTGAGAAGA+CGG + chr4_2:59758555-59758574 Msa0611540:intron 30.0%
ACGACATAAGTTTATCTGTT+AGG + chr4_2:59758601-59758620 Msa0611540:intron 30.0%
! AACCCCTGTTTGAAATATAA+TGG + chr4_2:59758745-59758764 Msa0611540:intron 30.0%
GTCCATTATATTTCAAACAG+GGG - chr4_2:59758750-59758769 None:intergenic 30.0%
AGTCAATAAAAACAAGATGC+TGG + chr4_2:59758823-59758842 Msa0611540:intron 30.0%
GTCAATAAAAACAAGATGCT+GGG + chr4_2:59758824-59758843 Msa0611540:intron 30.0%
TCAATAAAAACAAGATGCTG+GGG + chr4_2:59758825-59758844 Msa0611540:intron 30.0%
! AAAATGTTTCAGACTAGCTA+CGG + chr4_2:59758933-59758952 Msa0611540:intron 30.0%
TACTTAATAGTGAGCTATAG+TGG - chr4_2:59759004-59759023 None:intergenic 30.0%
AAAGATCAATGGAATACAAG+TGG - chr4_2:59759110-59759129 None:intergenic 30.0%
! TTTTTCTCGACTTCTCTATA+TGG + chr4_2:59759127-59759146 Msa0611540:intron 30.0%
! AATTTTCTGTTGCGTGATTT+TGG + chr4_2:59759171-59759190 Msa0611540:intron 30.0%
GTCATGATTCATATGACTAT+GGG - chr4_2:59759229-59759248 None:intergenic 30.0%
TGTCATGATTCATATGACTA+TGG - chr4_2:59759230-59759249 None:intergenic 30.0%
ATCATGACATCTAATTCTGT+AGG + chr4_2:59759241-59759260 Msa0611540:intron 30.0%
! GTAAAAGCACAATGAAACAA+TGG - chr4_2:59759280-59759299 None:intergenic 30.0%
ATGTGTATATTAGGAGTTGT+TGG + chr4_2:59759375-59759394 Msa0611540:intron 30.0%
! ATTTTATACCACGTTATCTC+TGG + chr4_2:59759483-59759502 Msa0611540:intron 30.0%
! TTTTATACCACGTTATCTCT+GGG + chr4_2:59759484-59759503 Msa0611540:intron 30.0%
GATAAGCCACATAAATAAGA+TGG + chr4_2:59759514-59759533 Msa0611540:intron 30.0%
! TGTACACCATCTTATTTATG+TGG - chr4_2:59759523-59759542 None:intergenic 30.0%
! AAGTAAGAATTCTGTTGCTT+TGG + chr4_2:59759557-59759576 Msa0611540:intron 30.0%
!! AGTAAGAATTCTGTTGCTTT+GGG + chr4_2:59759558-59759577 Msa0611540:intron 30.0%
! AGGTACCTTTTCAATAGAAA+AGG - chr4_2:59759677-59759696 None:intergenic 30.0%
!!! TGTTTTTGAAGTTGTCAACT+TGG + chr4_2:59759766-59759785 Msa0611540:intron 30.0%
!! TATTGATTTAATGGAGGACT+TGG + chr4_2:59759849-59759868 Msa0611540:three_prime_UTR 30.0%
! ATTGATTTAATGGAGGACTT+GGG + chr4_2:59759850-59759869 Msa0611540:three_prime_UTR 30.0%
AAGATGAATACAGTACAGTA+TGG - chr4_2:59759973-59759992 None:intergenic 30.0%
GAGTTATTGAGTAACAATCA+TGG - chr4_2:59760012-59760031 None:intergenic 30.0%
TCTCTCTAATCGTAAAAATG+TGG - chr4_2:59760043-59760062 None:intergenic 30.0%
TCATTTCCAGTCATTTCTTT+GGG - chr4_2:59760100-59760119 None:intergenic 30.0%
TTCATTTCCAGTCATTTCTT+TGG - chr4_2:59760101-59760120 None:intergenic 30.0%
AAAATCCCAAGAAGGAAAAT+AGG + chr4_2:59760120-59760139 Msa0611540:three_prime_UTR 30.0%
TGAAAGCTAAGGAGAGAAAA+TGG + chr4_2:59756726-59756745 Msa0611540:CDS 35.0%
AAATGGTCACGAAATAGAGA+AGG + chr4_2:59756743-59756762 Msa0611540:CDS 35.0%
TGGGTTATAGGATTGTAAAG+AGG + chr4_2:59757109-59757128 Msa0611540:intron 35.0%
GTTATAGGATTGTAAAGAGG+TGG + chr4_2:59757112-59757131 Msa0611540:intron 35.0%
GTAAAGAGGTGGTTTATGAA+TGG + chr4_2:59757123-59757142 Msa0611540:intron 35.0%
CTATAACAAAAGAGGGAAAG+AGG + chr4_2:59757190-59757209 Msa0611540:intron 35.0%
TTGCAAATGCATTCCTTGTA+TGG + chr4_2:59757217-59757236 Msa0611540:intron 35.0%
TGCAAATGCATTCCTTGTAT+GGG + chr4_2:59757218-59757237 Msa0611540:intron 35.0%
!!! TCGTTTTTTCCTTTTTGTGC+TGG + chr4_2:59757287-59757306 Msa0611540:intron 35.0%
!! ATAATTTGTTGGTAGCGGTA+AGG - chr4_2:59757447-59757466 None:intergenic 35.0%
! TCAGACATGTCTGTATTTTG+TGG - chr4_2:59757601-59757620 None:intergenic 35.0%
! GTATCTTTTACAAAGGCCAA+AGG - chr4_2:59757840-59757859 None:intergenic 35.0%
!! AGTACTGATGTACATACACT+AGG + chr4_2:59757861-59757880 Msa0611540:intron 35.0%
! ATTGCAAAACTGCATGCTTT+TGG - chr4_2:59758042-59758061 None:intergenic 35.0%
! CATGCAGTTTTGCAATTTGT+AGG + chr4_2:59758046-59758065 Msa0611540:intron 35.0%
TGCAATTTGTAGGGACTAAA+AGG + chr4_2:59758056-59758075 Msa0611540:intron 35.0%
GCAATTTGTAGGGACTAAAA+GGG + chr4_2:59758057-59758076 Msa0611540:intron 35.0%
CAATTTGTAGGGACTAAAAG+GGG + chr4_2:59758058-59758077 Msa0611540:intron 35.0%
AATTTGTAGGGACTAAAAGG+GGG + chr4_2:59758059-59758078 Msa0611540:intron 35.0%
!!! GTATGCAGCATCTTATTTTG+AGG + chr4_2:59758206-59758225 Msa0611540:intron 35.0%
!! CAAGTTTTATCTCACTAGGT+GGG - chr4_2:59758259-59758278 None:intergenic 35.0%
!! CAACCAAGTTTTATCTCACT+AGG - chr4_2:59758263-59758282 None:intergenic 35.0%
!! ACAATTTTGATGCAGGACTT+GGG - chr4_2:59758312-59758331 None:intergenic 35.0%
!! CATCTAGACAATTTTGATGC+AGG - chr4_2:59758319-59758338 None:intergenic 35.0%
CAAAATTATACCTTCTAGCG+AGG + chr4_2:59758641-59758660 Msa0611540:intron 35.0%
! TTATCCCAGTACGGAATTTT+TGG - chr4_2:59758723-59758742 None:intergenic 35.0%
TATTAATTCCAGTGCCTCTT+TGG - chr4_2:59758903-59758922 None:intergenic 35.0%
! AAATGTTTCAGACTAGCTAC+GGG + chr4_2:59758934-59758953 Msa0611540:intron 35.0%
CAAGTGGAAACATTCTACAT+CGG - chr4_2:59759094-59759113 None:intergenic 35.0%
GAAGTCGAGAAAAAGATCAA+TGG - chr4_2:59759121-59759140 None:intergenic 35.0%
! AAAGCACCTAAGAATCTATG+CGG - chr4_2:59759449-59759468 None:intergenic 35.0%
TTTATACCACGTTATCTCTG+GGG + chr4_2:59759485-59759504 Msa0611540:intron 35.0%
!! GTAAGAATTCTGTTGCTTTG+GGG + chr4_2:59759559-59759578 Msa0611540:intron 35.0%
ACAAGACAAGCATACAATTC+AGG - chr4_2:59759620-59759639 None:intergenic 35.0%
! ATATGCAAAGTGTCTTGCTT+TGG + chr4_2:59759644-59759663 Msa0611540:intron 35.0%
! GTACGCCTTTTCTATTGAAA+AGG + chr4_2:59759669-59759688 Msa0611540:intron 35.0%
AGTTGTCAACTTGGAATAAC+TGG + chr4_2:59759775-59759794 Msa0611540:intron 35.0%
TCTGCACATATCCTGAAAAA+CGG - chr4_2:59759810-59759829 None:intergenic 35.0%
! GTAATGGAAAACCGTTATGA+TGG + chr4_2:59759872-59759891 Msa0611540:three_prime_UTR 35.0%
GAGAACATCAACCATCATAA+CGG - chr4_2:59759886-59759905 None:intergenic 35.0%
CATTTCCAGTCATTTCTTTG+GGG - chr4_2:59760099-59760118 None:intergenic 35.0%
GGAAATGAAAAATCCCAAGA+AGG + chr4_2:59760112-59760131 Msa0611540:three_prime_UTR 35.0%
! GCTTTCCTATTTTCCTTCTT+GGG - chr4_2:59760128-59760147 None:intergenic 35.0%
! TGCTTTCCTATTTTCCTTCT+TGG - chr4_2:59760129-59760148 None:intergenic 35.0%
GAAGAATCAGGGTAAGTCAT+GGG - chr4_2:59756803-59756822 None:intergenic 40.0%
AGAAGAATCAGGGTAAGTCA+TGG - chr4_2:59756804-59756823 None:intergenic 40.0%
GAGAAACAGGAGAAGAATCA+GGG - chr4_2:59756814-59756833 None:intergenic 40.0%
! TCTAGGATACCTCAAGTTTG+GGG - chr4_2:59756868-59756887 None:intergenic 40.0%
! GTCTAGGATACCTCAAGTTT+GGG - chr4_2:59756869-59756888 None:intergenic 40.0%
! AGTCTAGGATACCTCAAGTT+TGG - chr4_2:59756870-59756889 None:intergenic 40.0%
AGTCAAACCAAAGAGAGTCT+AGG - chr4_2:59756885-59756904 None:intergenic 40.0%
TGCATTCCTTGTATGGGAAT+TGG + chr4_2:59757224-59757243 Msa0611540:intron 40.0%
GCATTACCAATTCCCATACA+AGG - chr4_2:59757233-59757252 None:intergenic 40.0%
AAACTCACACACCTTAAGTG+TGG + chr4_2:59757370-59757389 Msa0611540:intron 40.0%
ACACCTTAAGTGTGGATGAA+TGG + chr4_2:59757378-59757397 Msa0611540:intron 40.0%
TCTCCATTCATCCACACTTA+AGG - chr4_2:59757384-59757403 None:intergenic 40.0%
TGTGGATGAATGGAGAATCT+CGG + chr4_2:59757388-59757407 Msa0611540:intron 40.0%
TATATATGTAGCGAAGCCTG+AGG + chr4_2:59757474-59757493 Msa0611540:intron 40.0%
ATATATGTAGCGAAGCCTGA+GGG + chr4_2:59757475-59757494 Msa0611540:intron 40.0%
TGGCTAACATAGTCCATGAA+GGG + chr4_2:59757752-59757771 Msa0611540:intron 40.0%
TTTATCTCACTAGGTGGGTA+GGG - chr4_2:59758254-59758273 None:intergenic 40.0%
! TTTTATCTCACTAGGTGGGT+AGG - chr4_2:59758255-59758274 None:intergenic 40.0%
!! CCAAGTTTTATCTCACTAGG+TGG - chr4_2:59758260-59758279 None:intergenic 40.0%
CCACCTAGTGAGATAAAACT+TGG + chr4_2:59758257-59758276 Msa0611540:intron 40.0%
!! GACAATTTTGATGCAGGACT+TGG - chr4_2:59758313-59758332 None:intergenic 40.0%
AGCTCCAAAAATTCCGTACT+GGG + chr4_2:59758716-59758735 Msa0611540:intron 40.0%
CATTATCTCAGCCACAACTA+CGG - chr4_2:59758865-59758884 None:intergenic 40.0%
ATCCTACCACACTTTCATTG+TGG + chr4_2:59759415-59759434 Msa0611540:intron 40.0%
! TACCACAATGAAAGTGTGGT+AGG - chr4_2:59759420-59759439 None:intergenic 40.0%
TACCACACTTTCATTGTGGT+AGG + chr4_2:59759419-59759438 Msa0611540:intron 40.0%
TACCTACCACAATGAAAGTG+TGG - chr4_2:59759424-59759443 None:intergenic 40.0%
TTATACCACGTTATCTCTGG+GGG + chr4_2:59759486-59759505 Msa0611540:intron 40.0%
AGGAGTCTATCTCAAATCAG+TGG + chr4_2:59759588-59759607 Msa0611540:intron 40.0%
GGAGTCTATCTCAAATCAGT+GGG + chr4_2:59759589-59759608 Msa0611540:intron 40.0%
! CAACTTGGAATAACTGGTCT+TGG + chr4_2:59759781-59759800 Msa0611540:intron 40.0%
ATGTGCAGAAGATAACAGCT+CGG + chr4_2:59759821-59759840 Msa0611540:three_prime_UTR 40.0%
TTAATGGAGGACTTGGGTAA+TGG + chr4_2:59759856-59759875 Msa0611540:three_prime_UTR 40.0%
AAATAAAAAAACTGGGGGCG+AGG - chr4_2:59759915-59759934 None:intergenic 40.0%
CTAGTTCCCTTTCTTTACCT+CGG + chr4_2:59759943-59759962 Msa0611540:three_prime_UTR 40.0%
TAGTTCCCTTTCTTTACCTC+GGG + chr4_2:59759944-59759963 Msa0611540:three_prime_UTR 40.0%
! GGAAAGCATACAGAGATCTA+AGG + chr4_2:59760141-59760160 Msa0611540:three_prime_UTR 40.0%
GTGGATGAAGGTGAAAGCTA+AGG + chr4_2:59756715-59756734 Msa0611540:CDS 45.0%
TAAGTCATGGGAAGTGGAAG+AGG - chr4_2:59756791-59756810 None:intergenic 45.0%
GGAGAAACAGGAGAAGAATC+AGG - chr4_2:59756815-59756834 None:intergenic 45.0%
TCAACGACTTCAGGAGAAAC+AGG - chr4_2:59756827-59756846 None:intergenic 45.0%
! CAAGTTTGGGGTCACATTGA+AGG - chr4_2:59756856-59756875 None:intergenic 45.0%
TCAATGTGACCCCAAACTTG+AGG + chr4_2:59756856-59756875 Msa0611540:intron 45.0%
! GAGGTATCCTAGACTCTCTT+TGG + chr4_2:59756875-59756894 Msa0611540:intron 45.0%
GAGGTGGTTTATGAATGGAC+TGG + chr4_2:59757128-59757147 Msa0611540:intron 45.0%
AGCTTACATATCCGGATCCT+TGG - chr4_2:59757169-59757188 None:intergenic 45.0%
GTGGATGAATGGAGAATCTC+GGG + chr4_2:59757389-59757408 Msa0611540:intron 45.0%
AGAATCTCGGGTTCGAACTT+AGG + chr4_2:59757401-59757420 Msa0611540:intron 45.0%
GAATCTCGGGTTCGAACTTA+GGG + chr4_2:59757402-59757421 Msa0611540:intron 45.0%
CTGGCTAACATAGTCCATGA+AGG + chr4_2:59757751-59757770 Msa0611540:intron 45.0%
AAGTTTCTCTCCTCGCTAGA+AGG - chr4_2:59758654-59758673 None:intergenic 45.0%
!!! CAGTACGGAATTTTTGGAGC+TGG - chr4_2:59758717-59758736 None:intergenic 45.0%
CAGCTCCAAAAATTCCGTAC+TGG + chr4_2:59758715-59758734 Msa0611540:intron 45.0%
!! AGGGGTTAGTTATCCCAGTA+CGG - chr4_2:59758732-59758751 None:intergenic 45.0%
AAAACAAGATGCTGGGGCTA+AGG + chr4_2:59758831-59758850 Msa0611540:intron 45.0%
! AGGTTTAAGTGCCGTAGTTG+TGG + chr4_2:59758851-59758870 Msa0611540:intron 45.0%
GGTACTCCGCATAGATTCTT+AGG + chr4_2:59759440-59759459 Msa0611540:intron 45.0%
GGATTCCCGAGGTAAAGAAA+GGG - chr4_2:59759952-59759971 None:intergenic 45.0%
TGGATTCCCGAGGTAAAGAA+AGG - chr4_2:59759953-59759972 None:intergenic 45.0%
! TCTTTGGGGAGGAAAGAAGT+GGG - chr4_2:59760085-59760104 None:intergenic 45.0%
! TTCTTTGGGGAGGAAAGAAG+TGG - chr4_2:59760086-59760105 None:intergenic 45.0%
TTCCTCCCCAAAGAAATGAC+TGG + chr4_2:59760091-59760110 Msa0611540:three_prime_UTR 45.0%
! TTCCAGTCATTTCTTTGGGG+AGG - chr4_2:59760096-59760115 None:intergenic 45.0%
!!! TAAAAAAAAAAACAATTTTT+TGG + chr4_2:59757902-59757921 Msa0611540:intron 5.0%
!!! ATAAAATTTACATTTTTTTT+TGG - chr4_2:59758093-59758112 None:intergenic 5.0%
!!! AAAAAAAATGTAAATTTTAT+AGG + chr4_2:59758093-59758112 Msa0611540:intron 5.0%
!!! AAAAAAATGTAAATTTTATA+GGG + chr4_2:59758094-59758113 Msa0611540:intron 5.0%
!! TAAAATTAAATAAAAAAACT+GGG - chr4_2:59759922-59759941 None:intergenic 5.0%
!! ATAAAATTAAATAAAAAAAC+TGG - chr4_2:59759923-59759942 None:intergenic 5.0%
AAGAGACGAGGGATAGAGGT+GGG - chr4_2:59756620-59756639 None:intergenic 50.0%
TCAGGGTAAGTCATGGGAAG+TGG - chr4_2:59756797-59756816 None:intergenic 50.0%
AGGCGTTCATCAACGACTTC+AGG - chr4_2:59756836-59756855 None:intergenic 50.0%
TTGTGGCTGAGATAATGCCC+TGG + chr4_2:59758868-59758887 Msa0611540:intron 50.0%
ACTATCCCCCAGAGATAACG+TGG - chr4_2:59759494-59759513 None:intergenic 50.0%
!!! GGTCTTGGTGTCCGTTTTTC+AGG + chr4_2:59759796-59759815 Msa0611540:intron 50.0%
CAGTACAGTATGGATTCCCG+AGG - chr4_2:59759963-59759982 None:intergenic 50.0%
! CTTTGGGGAGGAAAGAAGTG+GGG - chr4_2:59760084-59760103 None:intergenic 50.0%
!! AGCACGGTAGCGGAAGATGA+GGG - chr4_2:59756573-59756592 None:intergenic 55.0%
!! AAGCACGGTAGCGGAAGATG+AGG - chr4_2:59756574-59756593 None:intergenic 55.0%
AAAGGTCTCCGGCAGAAGCA+CGG - chr4_2:59756589-59756608 None:intergenic 55.0%
GAAGAGACGAGGGATAGAGG+TGG - chr4_2:59756621-59756640 None:intergenic 55.0%
CTGGAAGAGACGAGGGATAG+AGG - chr4_2:59756624-59756643 None:intergenic 55.0%
AGAGCGACTGGAAGAGACGA+GGG - chr4_2:59756631-59756650 None:intergenic 55.0%
TGTCGTAGCTGCCAAGGATC+CGG + chr4_2:59757155-59757174 Msa0611540:intron 55.0%
CGGTAAGGACGCTGACATGT+TGG - chr4_2:59757432-59757451 None:intergenic 55.0%
TGAGCGCTCTCAAATCCCTC+AGG - chr4_2:59757493-59757512 None:intergenic 55.0%
! AGGGACTAAAAGGGGGCACA+AGG + chr4_2:59758066-59758085 Msa0611540:intron 55.0%
TCTCACTAGGTGGGTAGGGT+TGG - chr4_2:59758250-59758269 None:intergenic 55.0%
CTCTTTGGTGAAGCCTGCCA+GGG - chr4_2:59758888-59758907 None:intergenic 55.0%
! AGGCTTCACCAAAGAGGCAC+TGG + chr4_2:59758892-59758911 Msa0611540:intron 55.0%
! TACGGGCTATATGCGTGCGT+TGG + chr4_2:59758951-59758970 Msa0611540:intron 55.0%
! ACGGGCTATATGCGTGCGTT+GGG + chr4_2:59758952-59758971 Msa0611540:intron 55.0%
!! CTGTTGCTTTGGGGTCACGT+AGG + chr4_2:59759568-59759587 Msa0611540:intron 55.0%
AAAACTGGGGGCGAGGTTAG+TGG - chr4_2:59759908-59759927 None:intergenic 55.0%
TTCCGCTACCGTGCTTCTGC+CGG + chr4_2:59756578-59756597 Msa0611540:CDS 60.0%
GAGAGCGACTGGAAGAGACG+AGG - chr4_2:59756632-59756651 None:intergenic 60.0%
CTTCATCCACTCGTTCCCGC+CGG - chr4_2:59756705-59756724 None:intergenic 60.0%
! GGCAAGTGTCGTAGCTGCCA+AGG + chr4_2:59757149-59757168 Msa0611540:intron 60.0%
GGCTGAGATAATGCCCTGGC+AGG + chr4_2:59758872-59758891 Msa0611540:intron 60.0%
CCTCTTTGGTGAAGCCTGCC+AGG - chr4_2:59758889-59758908 None:intergenic 60.0%
CCTGGCAGGCTTCACCAAAG+AGG + chr4_2:59758886-59758905 Msa0611540:intron 60.0%
GCTATATGCGTGCGTTGGGC+TGG + chr4_2:59758956-59758975 Msa0611540:intron 60.0%
!! GTGCGTTGGGCTGGTTGTCA+TGG + chr4_2:59758965-59758984 Msa0611540:intron 60.0%
!! CGTTGGGCTGGTTGTCATGG+CGG + chr4_2:59758968-59758987 Msa0611540:intron 60.0%
! CTCCGGCAGAAGCACGGTAG+CGG - chr4_2:59756583-59756602 None:intergenic 65.0%
CTGCCGGAGACCTTTCCCCA+CGG + chr4_2:59756594-59756613 Msa0611540:CDS 65.0%
TAGAGGTGGGCCGTGGGGAA+AGG - chr4_2:59756607-59756626 None:intergenic 65.0%
AGGGATAGAGGTGGGCCGTG+GGG - chr4_2:59756612-59756631 None:intergenic 65.0%
GAGGGATAGAGGTGGGCCGT+GGG - chr4_2:59756613-59756632 None:intergenic 65.0%
TCTACGACGGCCGAGGTGGT+GGG - chr4_2:59756659-59756678 None:intergenic 65.0%
TTCTACGACGGCCGAGGTGG+TGG - chr4_2:59756660-59756679 None:intergenic 65.0%
ACCACCTCGGCCGTCGTAGA+AGG + chr4_2:59756659-59756678 Msa0611540:CDS 65.0%
ACCTTCTACGACGGCCGAGG+TGG - chr4_2:59756663-59756682 None:intergenic 65.0%
ACCTCGGCCGTCGTAGAAGG+TGG + chr4_2:59756662-59756681 Msa0611540:CDS 65.0%
AGCGGCGCCACCTTCTACGA+CGG - chr4_2:59756672-59756691 None:intergenic 65.0%
CGGCGGGAACGAGTGGATGA+AGG + chr4_2:59756703-59756722 Msa0611540:CDS 65.0%
GGGCCGTGGGGAAAGGTCTC+CGG - chr4_2:59756600-59756619 None:intergenic 70.0%
CGAGGGATAGAGGTGGGCCG+TGG - chr4_2:59756614-59756633 None:intergenic 70.0%
TGGTGGGGAGGGAGAGCGAC+TGG - chr4_2:59756643-59756662 None:intergenic 70.0%
GCTCTCCCTCCCCACCACCT+CGG + chr4_2:59756646-59756665 Msa0611540:CDS 70.0%
CTACGACGGCCGAGGTGGTG+GGG - chr4_2:59756658-59756677 None:intergenic 70.0%
GCCACCTTCTACGACGGCCG+AGG - chr4_2:59756666-59756685 None:intergenic 70.0%
!! GACGGCCGAGGTGGTGGGGA+GGG - chr4_2:59756654-59756673 None:intergenic 75.0%
!!! CGACGGCCGAGGTGGTGGGG+AGG - chr4_2:59756655-59756674 None:intergenic 80.0%
! GGCGCCGCTGCTAGACGCGC+CGG + chr4_2:59756683-59756702 Msa0611540:CDS 80.0%
! GCCGCTGCTAGACGCGCCGG+CGG + chr4_2:59756686-59756705 Msa0611540:CDS 80.0%
! CCCGCCGGCGCGTCTAGCAG+CGG - chr4_2:59756690-59756709 None:intergenic 80.0%
!! CCGCTGCTAGACGCGCCGGC+GGG + chr4_2:59756687-59756706 Msa0611540:CDS 80.0%
!! GACGCGCCGGCGGGAACGAG+TGG + chr4_2:59756696-59756715 Msa0611540:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 59756545 59760221 59756545 ID=Msa0611540;Name=Msa0611540
chr4_2 mRNA 59756545 59760221 59756545 ID=Msa0611540-mRNA-1;Parent=Msa0611540;Name=Msa0611540-mRNA-1;_AED=0.29;_eAED=0.30;_QI=0|0|0.5|1|0|0.5|2|402|105
chr4_2 exon 59756545 59756860 59756545 ID=Msa0611540-mRNA-1:exon:15888;Parent=Msa0611540-mRNA-1
chr4_2 exon 59759818 59760221 59759818 ID=Msa0611540-mRNA-1:exon:15889;Parent=Msa0611540-mRNA-1
chr4_2 CDS 59756545 59756860 59756545 ID=Msa0611540-mRNA-1:cds;Parent=Msa0611540-mRNA-1
chr4_2 CDS 59759818 59759819 59759818 ID=Msa0611540-mRNA-1:cds;Parent=Msa0611540-mRNA-1
chr4_2 three_prime_UTR 59759820 59760221 59759820 ID=Msa0611540-mRNA-1:three_prime_utr;Parent=Msa0611540-mRNA-1
Gene Sequence

>Msa0611540

ATGTTATCTACTACGAATGCAAGTTCCCTCATCTTCCGCTACCGTGCTTCTGCCGGAGACCTTTCCCCACGGCCCACCTCTATCCCTCGTCTCTTCCAGTCGCTCTCCCTCCCCACCACCTCGGCCGTCGTAGAAGGTGGCGCCGCTGCTAGACGCGCCGGCGGGAACGAGTGGATGAAGGTGAAAGCTAAGGAGAGAAAATGGTCACGAAATAGAGAAGGATATATGGTTGATAATGGTGAGCCTCTTCCACTTCCCATGACTTACCCTGATTCTTCTCCTGTTTCTCCTGAAGTCGTTGATGAACGCCTTCAATGA
Protein sequence

>Msa0611540

MLSTTNASSLIFRYRASAGDLSPRPTSIPRLFQSLSLPTTSAVVEGGAAARRAGGNEWMKVKAKERKWSRNREGYMVDNGEPLPLPMTYPDSSPVSPEVVDERLQ*