AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0853610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0853610 MtrunA17_Chr3g0111961 40.972 144 34 6 6 102 272 411 3.48e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0853610 AT5G13840.1 35.507 138 41 5 12 102 270 406 3.50e-14 67.0
Msa0853610 AT5G13840.2 35.507 138 41 5 12 102 270 406 3.50e-14 67.0

Find 58 sgRNAs with CRISPR-Local

Find 287 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCCTACTTATGGTGTTGC+TGG 0.242987 5_4:+45497925 Msa0853610:CDS
GGAGATTTGGCCCCTACTTA+TGG 0.262761 5_4:+45497916 Msa0853610:CDS
AAGGTTTCAAGTCACTTTAT+TGG 0.296214 5_4:+45497100 Msa0853610:CDS
GAGTCAAAACTTTGGAGATT+TGG 0.305615 5_4:+45497903 Msa0853610:intron
AAGTAGGTTTCAGTTATTAT+TGG 0.344035 5_4:-45498272 None:intergenic
ATTGTGGATATTATGCATTT+TGG 0.375498 5_4:+45498298 Msa0853610:three_prime_UTR
GCCCCTGCATATGATGTTGC+TGG 0.399655 5_4:+45497962 Msa0853610:three_prime_UTR
CACATAATATTAGATACTTT+AGG 0.409527 5_4:-45498144 None:intergenic
GCTTGTTAGCCATAAGTTTG+AGG 0.444684 5_4:+45497126 Msa0853610:CDS
AGAAACCTTGTGTCCGGAGG+TGG 0.472113 5_4:+45497677 Msa0853610:CDS
AAGAACTTGCCTCACGAAGA+TGG 0.472116 5_4:-45496308 None:intergenic
CACTATAGAAACCTTGTGTC+CGG 0.474110 5_4:+45497671 Msa0853610:CDS
AAACTATTTATGCACCATGT+AGG 0.481980 5_4:-45498340 None:intergenic
TGCAACTAGAGTCAAAACTT+TGG 0.486175 5_4:+45497895 Msa0853610:intron
CTTTCAGTTTCTACAAACAT+TGG 0.488573 5_4:-45496342 None:intergenic
CCGTACGCCGTAATCTTTCG+TGG 0.490584 5_4:+45496278 Msa0853610:five_prime_UTR
GTGAGCTACAAATGCTACAT+TGG 0.499048 5_4:+45497759 Msa0853610:CDS
GAGACGACCACGAAAGATTA+CGG 0.506427 5_4:-45496285 None:intergenic
TGAGCTACAAATGCTACATT+GGG 0.509106 5_4:+45497760 Msa0853610:CDS
TCAAACTAGTTGCAGACACT+TGG 0.509703 5_4:-45498399 None:intergenic
TTTCCAGCAACATCATATGC+AGG 0.512587 5_4:-45497965 None:intergenic
ATCCAGCAACACCATAAGTA+GGG 0.512928 5_4:-45497927 None:intergenic
AATCCAGCAACACCATAAGT+AGG 0.515125 5_4:-45497928 None:intergenic
AGAACTTGCCTCACGAAGAT+GGG 0.520489 5_4:-45496307 None:intergenic
AGAAGTGTTGAGGTTCAAGT+AGG 0.527053 5_4:-45496230 None:intergenic
ATTTCTTGCTTTCATTTCGA+AGG 0.528023 5_4:-45498022 None:intergenic
CTGAAACCTACTTCGGATTG+TGG 0.528174 5_4:+45498282 Msa0853610:three_prime_UTR
CCTATCAGCAGTTCCACCTC+CGG 0.534257 5_4:-45497690 None:intergenic
ATAATAACTGAAACCTACTT+CGG 0.550970 5_4:+45498275 Msa0853610:three_prime_UTR
CTACAAACATTGGAGACGGC+GGG 0.551950 5_4:-45496332 None:intergenic
CCGGAGGTGGAACTGCTGAT+AGG 0.556420 5_4:+45497690 Msa0853610:CDS
CAATGTTTGTAGAAACTGAA+AGG 0.559874 5_4:+45496343 Msa0853610:exon
TTCCAGCAACATCATATGCA+GGG 0.565901 5_4:-45497964 None:intergenic
CATACTTCAACATGACATGA+AGG 0.569136 5_4:+45497081 Msa0853610:CDS
TATTATTGGCATTATATGAG+TGG 0.573874 5_4:-45498258 None:intergenic
ACACAAGGTTTCTATAGTGA+GGG 0.575915 5_4:-45497667 None:intergenic
TAATATCCACAATCCGAAGT+AGG 0.579849 5_4:-45498288 None:intergenic
TACAATGATAAATTCCACAT+TGG 0.580711 5_4:+45498183 Msa0853610:three_prime_UTR
CGAAAGATTACGGCGTACGG+TGG 0.583408 5_4:-45496275 None:intergenic
GTTTCTACAAACATTGGAGA+CGG 0.585332 5_4:-45496336 None:intergenic
CAGTTCCACCTCCGGACACA+AGG 0.586305 5_4:-45497682 None:intergenic
CTACTGTGAAGGCTAGTGCT+TGG 0.589532 5_4:+45497642 Msa0853610:intron
AAACAAATGTTGCACCATGT+AGG 0.597669 5_4:-45498375 None:intergenic
CCACGAAAGATTACGGCGTA+CGG 0.599046 5_4:-45496278 None:intergenic
GACACAAGGTTTCTATAGTG+AGG 0.612944 5_4:-45497668 None:intergenic
GAAGTGTTGAGGTTCAAGTA+GGG 0.613175 5_4:-45496229 None:intergenic
GAAAGATTACGGCGTACGGT+GGG 0.624999 5_4:-45496274 None:intergenic
ATGACGAGAGAGAAGTGTTG+AGG 0.646451 5_4:-45496240 None:intergenic
AAATTTCAAATATACCAATG+TGG 0.646491 5_4:-45498197 None:intergenic
GCTACAAATGCTACATTGGG+AGG 0.650591 5_4:+45497763 Msa0853610:CDS
TCCAGCAACACCATAAGTAG+GGG 0.651113 5_4:-45497926 None:intergenic
TCCAGCAACATCATATGCAG+GGG 0.653662 5_4:-45497963 None:intergenic
TCTACAAACATTGGAGACGG+CGG 0.659466 5_4:-45496333 None:intergenic
TATAGAAACCTTGTGTCCGG+AGG 0.669981 5_4:+45497674 Msa0853610:CDS
GGTCGTCTCCCATCTTCGTG+AGG 0.670187 5_4:+45496299 Msa0853610:five_prime_UTR
CACAAGGTTTCTATAGTGAG+GGG 0.679776 5_4:-45497666 None:intergenic
GTAGCTCACAAGACACAAGA+CGG 0.701155 5_4:-45497745 None:intergenic
GCTCACAAGACACAAGACGG+TGG 0.711333 5_4:-45497742 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGTAAATTTTTTAGAA+TGG + chr5_4:45496757-45496776 Msa0853610:intron 10.0%
!!! AATTTGTAAATTTTTTAGAA+TGG + chr5_4:45496757-45496776 Msa0853610:intron 10.0%
!!! TTTGAAATTTGAAAAAACTA+TGG + chr5_4:45498210-45498229 Msa0853610:three_prime_UTR 15.0%
!!! TTTGAAATTTGAAAAAACTA+TGG + chr5_4:45498210-45498229 Msa0853610:three_prime_UTR 15.0%
!! TAGTGAAAGACTTAAATTAT+AGG - chr5_4:45496410-45496429 None:intergenic 20.0%
!!! GAAAAAATTAGGGTTTTTAT+GGG - chr5_4:45496466-45496485 None:intergenic 20.0%
!!! AAATTTACAAATTTTACTCC+TGG - chr5_4:45496750-45496769 None:intergenic 20.0%
!! ATTCATACTTTCTTTAACAT+TGG + chr5_4:45496820-45496839 Msa0853610:intron 20.0%
!! ATTAAGAGACCAATATAAAT+TGG - chr5_4:45496865-45496884 None:intergenic 20.0%
!! TCATAATGATATGCATTAAT+AGG + chr5_4:45496928-45496947 Msa0853610:intron 20.0%
!!! ATTTTTTTACCTCAAACTTA+TGG - chr5_4:45497138-45497157 None:intergenic 20.0%
!!! CAATGTCATGTTTTTTTATT+CGG + chr5_4:45497362-45497381 Msa0853610:intron 20.0%
!! TAATTATTACGAATGTTGAA+AGG + chr5_4:45497391-45497410 Msa0853610:intron 20.0%
!!! TATTTTTTGCTTTTCCTTTT+AGG + chr5_4:45497800-45497819 Msa0853610:intron 20.0%
!!! TTTTTTGCTATTGTGATTTT+AGG + chr5_4:45497855-45497874 Msa0853610:intron 20.0%
!! CACATAATATTAGATACTTT+AGG - chr5_4:45498147-45498166 None:intergenic 20.0%
!! AAATTTCAAATATACCAATG+TGG - chr5_4:45498200-45498219 None:intergenic 20.0%
!! TAGTGAAAGACTTAAATTAT+AGG - chr5_4:45496410-45496429 None:intergenic 20.0%
!!! GAAAAAATTAGGGTTTTTAT+GGG - chr5_4:45496466-45496485 None:intergenic 20.0%
!!! AAATTTACAAATTTTACTCC+TGG - chr5_4:45496750-45496769 None:intergenic 20.0%
!! ATTCATACTTTCTTTAACAT+TGG + chr5_4:45496820-45496839 Msa0853610:intron 20.0%
!! ATTAAGAGACCAATATAAAT+TGG - chr5_4:45496865-45496884 None:intergenic 20.0%
!! TCATAATGATATGCATTAAT+AGG + chr5_4:45496928-45496947 Msa0853610:intron 20.0%
!!! ATTTTTTTACCTCAAACTTA+TGG - chr5_4:45497138-45497157 None:intergenic 20.0%
!!! CAATGTCATGTTTTTTTATT+CGG + chr5_4:45497362-45497381 Msa0853610:intron 20.0%
!! TAATTATTACGAATGTTGAA+AGG + chr5_4:45497391-45497410 Msa0853610:intron 20.0%
!!! TATTTTTTGCTTTTCCTTTT+AGG + chr5_4:45497800-45497819 Msa0853610:intron 20.0%
!!! TTTTTTGCTATTGTGATTTT+AGG + chr5_4:45497855-45497874 Msa0853610:intron 20.0%
!! CACATAATATTAGATACTTT+AGG - chr5_4:45498147-45498166 None:intergenic 20.0%
!! AAATTTCAAATATACCAATG+TGG - chr5_4:45498200-45498219 None:intergenic 20.0%
!! GGAAAAAATTAGGGTTTTTA+TGG - chr5_4:45496467-45496486 None:intergenic 25.0%
!! TATTAATTTTTCGTGTGTCA+AGG + chr5_4:45496517-45496536 Msa0853610:intron 25.0%
!!! CTAATTTTTTGTGTGTATCA+AGG + chr5_4:45496565-45496584 Msa0853610:intron 25.0%
!!! TGATATATTTGGCTTTTAAC+TGG + chr5_4:45496712-45496731 Msa0853610:intron 25.0%
! ATACCAGTTCCAATTTATAT+TGG + chr5_4:45496853-45496872 Msa0853610:intron 25.0%
! TCTTAATATTGTAACCATGA+GGG + chr5_4:45496878-45496897 Msa0853610:intron 25.0%
!! AAAAGTAATTTTGTGTGAGT+TGG - chr5_4:45497300-45497319 None:intergenic 25.0%
! AAAACATGACATTGAACAAA+AGG - chr5_4:45497356-45497375 None:intergenic 25.0%
!!! CACATGTTTTACTTTGAAAA+AGG - chr5_4:45497506-45497525 None:intergenic 25.0%
! AATATGAAAACCAACCTAAA+AGG - chr5_4:45497817-45497836 None:intergenic 25.0%
!! TTGCTTCATATTTTGTAAAG+AGG + chr5_4:45497990-45498009 Msa0853610:three_prime_UTR 25.0%
! TACAATGATAAATTCCACAT+TGG + chr5_4:45498183-45498202 Msa0853610:three_prime_UTR 25.0%
! TATTATTGGCATTATATGAG+TGG - chr5_4:45498261-45498280 None:intergenic 25.0%
! AAGTAGGTTTCAGTTATTAT+TGG - chr5_4:45498275-45498294 None:intergenic 25.0%
! ATAATAACTGAAACCTACTT+CGG + chr5_4:45498275-45498294 Msa0853610:three_prime_UTR 25.0%
!! ATTGTGGATATTATGCATTT+TGG + chr5_4:45498298-45498317 Msa0853610:three_prime_UTR 25.0%
!! TTTTTAATGTCAAACCTACA+TGG + chr5_4:45498326-45498345 Msa0853610:three_prime_UTR 25.0%
!! TTTTTAATGTCAAACCTACA+TGG + chr5_4:45498326-45498345 Msa0853610:three_prime_UTR 25.0%
!! GGAAAAAATTAGGGTTTTTA+TGG - chr5_4:45496467-45496486 None:intergenic 25.0%
!! TATTAATTTTTCGTGTGTCA+AGG + chr5_4:45496517-45496536 Msa0853610:intron 25.0%
!!! CTAATTTTTTGTGTGTATCA+AGG + chr5_4:45496565-45496584 Msa0853610:intron 25.0%
!!! TGATATATTTGGCTTTTAAC+TGG + chr5_4:45496712-45496731 Msa0853610:intron 25.0%
! ATACCAGTTCCAATTTATAT+TGG + chr5_4:45496853-45496872 Msa0853610:intron 25.0%
! TCTTAATATTGTAACCATGA+GGG + chr5_4:45496878-45496897 Msa0853610:intron 25.0%
!! AAAAGTAATTTTGTGTGAGT+TGG - chr5_4:45497300-45497319 None:intergenic 25.0%
! AAAACATGACATTGAACAAA+AGG - chr5_4:45497356-45497375 None:intergenic 25.0%
!!! CACATGTTTTACTTTGAAAA+AGG - chr5_4:45497506-45497525 None:intergenic 25.0%
! AATATGAAAACCAACCTAAA+AGG - chr5_4:45497817-45497836 None:intergenic 25.0%
!! TTGCTTCATATTTTGTAAAG+AGG + chr5_4:45497990-45498009 Msa0853610:three_prime_UTR 25.0%
! TACAATGATAAATTCCACAT+TGG + chr5_4:45498183-45498202 Msa0853610:three_prime_UTR 25.0%
! TATTATTGGCATTATATGAG+TGG - chr5_4:45498261-45498280 None:intergenic 25.0%
! AAGTAGGTTTCAGTTATTAT+TGG - chr5_4:45498275-45498294 None:intergenic 25.0%
! ATAATAACTGAAACCTACTT+CGG + chr5_4:45498275-45498294 Msa0853610:three_prime_UTR 25.0%
!! ATTGTGGATATTATGCATTT+TGG + chr5_4:45498298-45498317 Msa0853610:three_prime_UTR 25.0%
!! TTTTTAATGTCAAACCTACA+TGG + chr5_4:45498326-45498345 Msa0853610:three_prime_UTR 25.0%
!! TTTTTAATGTCAAACCTACA+TGG + chr5_4:45498326-45498345 Msa0853610:three_prime_UTR 25.0%
CTTTCAGTTTCTACAAACAT+TGG - chr5_4:45496345-45496364 None:intergenic 30.0%
CAATGTTTGTAGAAACTGAA+AGG + chr5_4:45496343-45496362 Msa0853610:exon 30.0%
GATGAGAAGGGAAAAAATTA+GGG - chr5_4:45496476-45496495 None:intergenic 30.0%
GCCTTTATGTGTGATATATT+TGG + chr5_4:45496701-45496720 Msa0853610:intron 30.0%
GCCAAATATATCACACATAA+AGG - chr5_4:45496705-45496724 None:intergenic 30.0%
AGACCAATATAAATTGGAAC+TGG - chr5_4:45496859-45496878 None:intergenic 30.0%
CTCTTAATATTGTAACCATG+AGG + chr5_4:45496877-45496896 Msa0853610:intron 30.0%
CTTAATATTGTAACCATGAG+GGG + chr5_4:45496879-45496898 Msa0853610:intron 30.0%
! AAGGTTTCAAGTCACTTTAT+TGG + chr5_4:45497100-45497119 Msa0853610:CDS 30.0%
GATAAAAATTGCCATCAAAC+AGG - chr5_4:45497195-45497214 None:intergenic 30.0%
! TTTTTATCCTTCAACTGGAT+AGG + chr5_4:45497207-45497226 Msa0853610:intron 30.0%
TTCTGGTAGTAATGATAATC+AGG + chr5_4:45497270-45497289 Msa0853610:intron 30.0%
! TTTCTTATATGAGCAGCTTT+TGG + chr5_4:45497570-45497589 Msa0853610:intron 30.0%
!! TTTTGCTTTTCCTTTTAGGT+TGG + chr5_4:45497804-45497823 Msa0853610:intron 30.0%
!! GTTGCTGGATTTTAAAGATT+TGG + chr5_4:45497940-45497959 Msa0853610:exon 30.0%
ATTTCTTGCTTTCATTTCGA+AGG - chr5_4:45498025-45498044 None:intergenic 30.0%
AAACTATTTATGCACCATGT+AGG - chr5_4:45498343-45498362 None:intergenic 30.0%
CTTTCAGTTTCTACAAACAT+TGG - chr5_4:45496345-45496364 None:intergenic 30.0%
CAATGTTTGTAGAAACTGAA+AGG + chr5_4:45496343-45496362 Msa0853610:exon 30.0%
GATGAGAAGGGAAAAAATTA+GGG - chr5_4:45496476-45496495 None:intergenic 30.0%
GCCTTTATGTGTGATATATT+TGG + chr5_4:45496701-45496720 Msa0853610:intron 30.0%
GCCAAATATATCACACATAA+AGG - chr5_4:45496705-45496724 None:intergenic 30.0%
AGACCAATATAAATTGGAAC+TGG - chr5_4:45496859-45496878 None:intergenic 30.0%
CTCTTAATATTGTAACCATG+AGG + chr5_4:45496877-45496896 Msa0853610:intron 30.0%
CTTAATATTGTAACCATGAG+GGG + chr5_4:45496879-45496898 Msa0853610:intron 30.0%
! AAGGTTTCAAGTCACTTTAT+TGG + chr5_4:45497100-45497119 Msa0853610:CDS 30.0%
GATAAAAATTGCCATCAAAC+AGG - chr5_4:45497195-45497214 None:intergenic 30.0%
! TTTTTATCCTTCAACTGGAT+AGG + chr5_4:45497207-45497226 Msa0853610:intron 30.0%
TTCTGGTAGTAATGATAATC+AGG + chr5_4:45497270-45497289 Msa0853610:intron 30.0%
! TTTCTTATATGAGCAGCTTT+TGG + chr5_4:45497570-45497589 Msa0853610:intron 30.0%
!! TTTTGCTTTTCCTTTTAGGT+TGG + chr5_4:45497804-45497823 Msa0853610:intron 30.0%
!! GTTGCTGGATTTTAAAGATT+TGG + chr5_4:45497940-45497959 Msa0853610:exon 30.0%
ATTTCTTGCTTTCATTTCGA+AGG - chr5_4:45498025-45498044 None:intergenic 30.0%
AAACTATTTATGCACCATGT+AGG - chr5_4:45498343-45498362 None:intergenic 30.0%
GTTTCTACAAACATTGGAGA+CGG - chr5_4:45496339-45496358 None:intergenic 35.0%
GGATGAGAAGGGAAAAAATT+AGG - chr5_4:45496477-45496496 None:intergenic 35.0%
TACCCAACCAAAGCAAAATT+GGG - chr5_4:45496499-45496518 None:intergenic 35.0%
ATACCCAACCAAAGCAAAAT+TGG - chr5_4:45496500-45496519 None:intergenic 35.0%
ATGTTCTATGCTTAACATGC+AGG + chr5_4:45496593-45496612 Msa0853610:intron 35.0%
! TATCACACATAAAGGCATGA+CGG - chr5_4:45496697-45496716 None:intergenic 35.0%
!! AACTGGTTTGTTAAGATTCC+AGG + chr5_4:45496729-45496748 Msa0853610:intron 35.0%
TAGATATAATAAGCCCCTCA+TGG - chr5_4:45496895-45496914 None:intergenic 35.0%
AGGGGCTTATTATATCTATC+TGG + chr5_4:45496897-45496916 Msa0853610:intron 35.0%
ACAACACCTGAAATATACTC+TGG + chr5_4:45496993-45497012 Msa0853610:intron 35.0%
CATACTTCAACATGACATGA+AGG + chr5_4:45497081-45497100 Msa0853610:CDS 35.0%
TAGCACTAATTCCTGTTTGA+TGG + chr5_4:45497181-45497200 Msa0853610:intron 35.0%
! GGCAATTTTTATCCTTCAAC+TGG + chr5_4:45497202-45497221 Msa0853610:intron 35.0%
! CACAACTCAACTTTGATTTC+TGG + chr5_4:45497455-45497474 Msa0853610:intron 35.0%
! AGGAAAAAACCTGTCATTTG+TGG - chr5_4:45497486-45497505 None:intergenic 35.0%
! TATATGAGCAGCTTTTGGTA+TGG + chr5_4:45497575-45497594 Msa0853610:intron 35.0%
! ATGTTAGTCTCAAAGTTGGT+TGG - chr5_4:45497613-45497632 None:intergenic 35.0%
! TGCTATGTTAGTCTCAAAGT+TGG - chr5_4:45497617-45497636 None:intergenic 35.0%
ACACAAGGTTTCTATAGTGA+GGG - chr5_4:45497670-45497689 None:intergenic 35.0%
! CTGATAGGTGTATTCTTTTC+TGG + chr5_4:45497705-45497724 Msa0853610:CDS 35.0%
TGAGCTACAAATGCTACATT+GGG + chr5_4:45497760-45497779 Msa0853610:CDS 35.0%
TGCAACTAGAGTCAAAACTT+TGG + chr5_4:45497895-45497914 Msa0853610:intron 35.0%
! GAGTCAAAACTTTGGAGATT+TGG + chr5_4:45497903-45497922 Msa0853610:intron 35.0%
TAATATCCACAATCCGAAGT+AGG - chr5_4:45498291-45498310 None:intergenic 35.0%
AAACAAATGTTGCACCATGT+AGG - chr5_4:45498378-45498397 None:intergenic 35.0%
GTTTCTACAAACATTGGAGA+CGG - chr5_4:45496339-45496358 None:intergenic 35.0%
GGATGAGAAGGGAAAAAATT+AGG - chr5_4:45496477-45496496 None:intergenic 35.0%
TACCCAACCAAAGCAAAATT+GGG - chr5_4:45496499-45496518 None:intergenic 35.0%
ATACCCAACCAAAGCAAAAT+TGG - chr5_4:45496500-45496519 None:intergenic 35.0%
ATGTTCTATGCTTAACATGC+AGG + chr5_4:45496593-45496612 Msa0853610:intron 35.0%
! TATCACACATAAAGGCATGA+CGG - chr5_4:45496697-45496716 None:intergenic 35.0%
!! AACTGGTTTGTTAAGATTCC+AGG + chr5_4:45496729-45496748 Msa0853610:intron 35.0%
TAGATATAATAAGCCCCTCA+TGG - chr5_4:45496895-45496914 None:intergenic 35.0%
AGGGGCTTATTATATCTATC+TGG + chr5_4:45496897-45496916 Msa0853610:intron 35.0%
ACAACACCTGAAATATACTC+TGG + chr5_4:45496993-45497012 Msa0853610:intron 35.0%
CATACTTCAACATGACATGA+AGG + chr5_4:45497081-45497100 Msa0853610:CDS 35.0%
TAGCACTAATTCCTGTTTGA+TGG + chr5_4:45497181-45497200 Msa0853610:intron 35.0%
! GGCAATTTTTATCCTTCAAC+TGG + chr5_4:45497202-45497221 Msa0853610:intron 35.0%
! CACAACTCAACTTTGATTTC+TGG + chr5_4:45497455-45497474 Msa0853610:intron 35.0%
! AGGAAAAAACCTGTCATTTG+TGG - chr5_4:45497486-45497505 None:intergenic 35.0%
! TATATGAGCAGCTTTTGGTA+TGG + chr5_4:45497575-45497594 Msa0853610:intron 35.0%
! ATGTTAGTCTCAAAGTTGGT+TGG - chr5_4:45497613-45497632 None:intergenic 35.0%
! TGCTATGTTAGTCTCAAAGT+TGG - chr5_4:45497617-45497636 None:intergenic 35.0%
ACACAAGGTTTCTATAGTGA+GGG - chr5_4:45497670-45497689 None:intergenic 35.0%
! CTGATAGGTGTATTCTTTTC+TGG + chr5_4:45497705-45497724 Msa0853610:CDS 35.0%
TGAGCTACAAATGCTACATT+GGG + chr5_4:45497760-45497779 Msa0853610:CDS 35.0%
TGCAACTAGAGTCAAAACTT+TGG + chr5_4:45497895-45497914 Msa0853610:intron 35.0%
! GAGTCAAAACTTTGGAGATT+TGG + chr5_4:45497903-45497922 Msa0853610:intron 35.0%
TAATATCCACAATCCGAAGT+AGG - chr5_4:45498291-45498310 None:intergenic 35.0%
AAACAAATGTTGCACCATGT+AGG - chr5_4:45498378-45498397 None:intergenic 35.0%
!! GAAGTGTTGAGGTTCAAGTA+GGG - chr5_4:45496232-45496251 None:intergenic 40.0%
!! AGAAGTGTTGAGGTTCAAGT+AGG - chr5_4:45496233-45496252 None:intergenic 40.0%
AGCAAAATTGGGGATGAGAA+GGG - chr5_4:45496488-45496507 None:intergenic 40.0%
AAGCAAAATTGGGGATGAGA+AGG - chr5_4:45496489-45496508 None:intergenic 40.0%
! TCTCATCCCCAATTTTGCTT+TGG + chr5_4:45496489-45496508 Msa0853610:intron 40.0%
! ATCCCCAATTTTGCTTTGGT+TGG + chr5_4:45496493-45496512 Msa0853610:intron 40.0%
! TCCCCAATTTTGCTTTGGTT+GGG + chr5_4:45496494-45496513 Msa0853610:intron 40.0%
ACCCAACCAAAGCAAAATTG+GGG - chr5_4:45496498-45496517 None:intergenic 40.0%
! TTTTCGTGTGTCAAGGATTC+AGG + chr5_4:45496524-45496543 Msa0853610:intron 40.0%
! CATAAAGGCATGACGGTAAA+TGG - chr5_4:45496690-45496709 None:intergenic 40.0%
ACACCTGAAATATACTCTGG+TGG + chr5_4:45496996-45497015 Msa0853610:intron 40.0%
CAACCACCAGAGTATATTTC+AGG - chr5_4:45497002-45497021 None:intergenic 40.0%
! GCTTGTTAGCCATAAGTTTG+AGG + chr5_4:45497126-45497145 Msa0853610:CDS 40.0%
AACTGGATAGGTATGTGGAT+AGG + chr5_4:45497219-45497238 Msa0853610:intron 40.0%
GATAGGTATGTGGATAGGAA+TGG + chr5_4:45497224-45497243 Msa0853610:intron 40.0%
!! GATGACAGATAACTTGCTTC+TGG + chr5_4:45497253-45497272 Msa0853610:intron 40.0%
TGAAAGGACGAAACACACAA+AGG + chr5_4:45497407-45497426 Msa0853610:intron 40.0%
GAAAGGACGAAACACACAAA+GGG + chr5_4:45497408-45497427 Msa0853610:intron 40.0%
AAAGGACGAAACACACAAAG+GGG + chr5_4:45497409-45497428 Msa0853610:intron 40.0%
CACACAAAGGGGTAATTGAA+AGG + chr5_4:45497420-45497439 Msa0853610:intron 40.0%
ATAGCATACTGCTACTGTGA+AGG + chr5_4:45497631-45497650 Msa0853610:intron 40.0%
CACAAGGTTTCTATAGTGAG+GGG - chr5_4:45497669-45497688 None:intergenic 40.0%
GACACAAGGTTTCTATAGTG+AGG - chr5_4:45497671-45497690 None:intergenic 40.0%
CACTATAGAAACCTTGTGTC+CGG + chr5_4:45497671-45497690 Msa0853610:CDS 40.0%
GTGAGCTACAAATGCTACAT+TGG + chr5_4:45497759-45497778 Msa0853610:CDS 40.0%
ATCCAGCAACACCATAAGTA+GGG - chr5_4:45497930-45497949 None:intergenic 40.0%
AATCCAGCAACACCATAAGT+AGG - chr5_4:45497931-45497950 None:intergenic 40.0%
TTCCAGCAACATCATATGCA+GGG - chr5_4:45497967-45497986 None:intergenic 40.0%
TTTCCAGCAACATCATATGC+AGG - chr5_4:45497968-45497987 None:intergenic 40.0%
!! GAAGTGTTGAGGTTCAAGTA+GGG - chr5_4:45496232-45496251 None:intergenic 40.0%
!! AGAAGTGTTGAGGTTCAAGT+AGG - chr5_4:45496233-45496252 None:intergenic 40.0%
AGCAAAATTGGGGATGAGAA+GGG - chr5_4:45496488-45496507 None:intergenic 40.0%
AAGCAAAATTGGGGATGAGA+AGG - chr5_4:45496489-45496508 None:intergenic 40.0%
! TCTCATCCCCAATTTTGCTT+TGG + chr5_4:45496489-45496508 Msa0853610:intron 40.0%
! ATCCCCAATTTTGCTTTGGT+TGG + chr5_4:45496493-45496512 Msa0853610:intron 40.0%
! TCCCCAATTTTGCTTTGGTT+GGG + chr5_4:45496494-45496513 Msa0853610:intron 40.0%
ACCCAACCAAAGCAAAATTG+GGG - chr5_4:45496498-45496517 None:intergenic 40.0%
! TTTTCGTGTGTCAAGGATTC+AGG + chr5_4:45496524-45496543 Msa0853610:intron 40.0%
! CATAAAGGCATGACGGTAAA+TGG - chr5_4:45496690-45496709 None:intergenic 40.0%
ACACCTGAAATATACTCTGG+TGG + chr5_4:45496996-45497015 Msa0853610:intron 40.0%
CAACCACCAGAGTATATTTC+AGG - chr5_4:45497002-45497021 None:intergenic 40.0%
! GCTTGTTAGCCATAAGTTTG+AGG + chr5_4:45497126-45497145 Msa0853610:CDS 40.0%
AACTGGATAGGTATGTGGAT+AGG + chr5_4:45497219-45497238 Msa0853610:intron 40.0%
GATAGGTATGTGGATAGGAA+TGG + chr5_4:45497224-45497243 Msa0853610:intron 40.0%
!! GATGACAGATAACTTGCTTC+TGG + chr5_4:45497253-45497272 Msa0853610:intron 40.0%
TGAAAGGACGAAACACACAA+AGG + chr5_4:45497407-45497426 Msa0853610:intron 40.0%
GAAAGGACGAAACACACAAA+GGG + chr5_4:45497408-45497427 Msa0853610:intron 40.0%
AAAGGACGAAACACACAAAG+GGG + chr5_4:45497409-45497428 Msa0853610:intron 40.0%
CACACAAAGGGGTAATTGAA+AGG + chr5_4:45497420-45497439 Msa0853610:intron 40.0%
ATAGCATACTGCTACTGTGA+AGG + chr5_4:45497631-45497650 Msa0853610:intron 40.0%
CACAAGGTTTCTATAGTGAG+GGG - chr5_4:45497669-45497688 None:intergenic 40.0%
GACACAAGGTTTCTATAGTG+AGG - chr5_4:45497671-45497690 None:intergenic 40.0%
CACTATAGAAACCTTGTGTC+CGG + chr5_4:45497671-45497690 Msa0853610:CDS 40.0%
GTGAGCTACAAATGCTACAT+TGG + chr5_4:45497759-45497778 Msa0853610:CDS 40.0%
ATCCAGCAACACCATAAGTA+GGG - chr5_4:45497930-45497949 None:intergenic 40.0%
AATCCAGCAACACCATAAGT+AGG - chr5_4:45497931-45497950 None:intergenic 40.0%
TTCCAGCAACATCATATGCA+GGG - chr5_4:45497967-45497986 None:intergenic 40.0%
TTTCCAGCAACATCATATGC+AGG - chr5_4:45497968-45497987 None:intergenic 40.0%
!! ATGACGAGAGAGAAGTGTTG+AGG - chr5_4:45496243-45496262 None:intergenic 45.0%
GAGACGACCACGAAAGATTA+CGG - chr5_4:45496288-45496307 None:intergenic 45.0%
AGAACTTGCCTCACGAAGAT+GGG - chr5_4:45496310-45496329 None:intergenic 45.0%
AAGAACTTGCCTCACGAAGA+TGG - chr5_4:45496311-45496330 None:intergenic 45.0%
TCTACAAACATTGGAGACGG+CGG - chr5_4:45496336-45496355 None:intergenic 45.0%
!! TTTTTCAGCAACGATGAGGG+AGG - chr5_4:45496376-45496395 None:intergenic 45.0%
!! GCGTTTTTCAGCAACGATGA+GGG - chr5_4:45496379-45496398 None:intergenic 45.0%
!! TGCGTTTTTCAGCAACGATG+AGG - chr5_4:45496380-45496399 None:intergenic 45.0%
TCATCGTTGCTGAAAAACGC+AGG + chr5_4:45496379-45496398 Msa0853610:CDS 45.0%
! GCATGGAACTCTCGCATTTT+TGG + chr5_4:45497036-45497055 Msa0853610:intron 45.0%
!!! ACTCTCGCATTTTTGGCTTC+AGG + chr5_4:45497043-45497062 Msa0853610:intron 45.0%
TGGCTTCAGGCAGTATGTAT+AGG + chr5_4:45497056-45497075 Msa0853610:intron 45.0%
CCACATACCTATCCAGTTGA+AGG - chr5_4:45497217-45497236 None:intergenic 45.0%
CCTTCAACTGGATAGGTATG+TGG + chr5_4:45497214-45497233 Msa0853610:intron 45.0%
CTGGCAAAACCACAAATGAC+AGG + chr5_4:45497474-45497493 Msa0853610:intron 45.0%
TATAGAAACCTTGTGTCCGG+AGG + chr5_4:45497674-45497693 Msa0853610:CDS 45.0%
GTAGCTCACAAGACACAAGA+CGG - chr5_4:45497748-45497767 None:intergenic 45.0%
GCTACAAATGCTACATTGGG+AGG + chr5_4:45497763-45497782 Msa0853610:CDS 45.0%
TCCAGCAACACCATAAGTAG+GGG - chr5_4:45497929-45497948 None:intergenic 45.0%
TCCAGCAACATCATATGCAG+GGG - chr5_4:45497966-45497985 None:intergenic 45.0%
CTGAAACCTACTTCGGATTG+TGG + chr5_4:45498282-45498301 Msa0853610:three_prime_UTR 45.0%
!! ATGACGAGAGAGAAGTGTTG+AGG - chr5_4:45496243-45496262 None:intergenic 45.0%
GAGACGACCACGAAAGATTA+CGG - chr5_4:45496288-45496307 None:intergenic 45.0%
AGAACTTGCCTCACGAAGAT+GGG - chr5_4:45496310-45496329 None:intergenic 45.0%
AAGAACTTGCCTCACGAAGA+TGG - chr5_4:45496311-45496330 None:intergenic 45.0%
TCTACAAACATTGGAGACGG+CGG - chr5_4:45496336-45496355 None:intergenic 45.0%
!! TTTTTCAGCAACGATGAGGG+AGG - chr5_4:45496376-45496395 None:intergenic 45.0%
!! GCGTTTTTCAGCAACGATGA+GGG - chr5_4:45496379-45496398 None:intergenic 45.0%
!! TGCGTTTTTCAGCAACGATG+AGG - chr5_4:45496380-45496399 None:intergenic 45.0%
TCATCGTTGCTGAAAAACGC+AGG + chr5_4:45496379-45496398 Msa0853610:CDS 45.0%
! GCATGGAACTCTCGCATTTT+TGG + chr5_4:45497036-45497055 Msa0853610:intron 45.0%
!!! ACTCTCGCATTTTTGGCTTC+AGG + chr5_4:45497043-45497062 Msa0853610:intron 45.0%
TGGCTTCAGGCAGTATGTAT+AGG + chr5_4:45497056-45497075 Msa0853610:intron 45.0%
CCACATACCTATCCAGTTGA+AGG - chr5_4:45497217-45497236 None:intergenic 45.0%
CCTTCAACTGGATAGGTATG+TGG + chr5_4:45497214-45497233 Msa0853610:intron 45.0%
CTGGCAAAACCACAAATGAC+AGG + chr5_4:45497474-45497493 Msa0853610:intron 45.0%
TATAGAAACCTTGTGTCCGG+AGG + chr5_4:45497674-45497693 Msa0853610:CDS 45.0%
GTAGCTCACAAGACACAAGA+CGG - chr5_4:45497748-45497767 None:intergenic 45.0%
GCTACAAATGCTACATTGGG+AGG + chr5_4:45497763-45497782 Msa0853610:CDS 45.0%
TCCAGCAACACCATAAGTAG+GGG - chr5_4:45497929-45497948 None:intergenic 45.0%
TCCAGCAACATCATATGCAG+GGG - chr5_4:45497966-45497985 None:intergenic 45.0%
CTGAAACCTACTTCGGATTG+TGG + chr5_4:45498282-45498301 Msa0853610:three_prime_UTR 45.0%
GAAAGATTACGGCGTACGGT+GGG - chr5_4:45496277-45496296 None:intergenic 50.0%
CCACGAAAGATTACGGCGTA+CGG - chr5_4:45496281-45496300 None:intergenic 50.0%
CTACAAACATTGGAGACGGC+GGG - chr5_4:45496335-45496354 None:intergenic 50.0%
CTGGTGGTTGTGAATAGAGC+TGG + chr5_4:45497012-45497031 Msa0853610:intron 50.0%
TTGTGAATAGAGCTGGCGCA+TGG + chr5_4:45497019-45497038 Msa0853610:intron 50.0%
CTACTGTGAAGGCTAGTGCT+TGG + chr5_4:45497642-45497661 Msa0853610:intron 50.0%
GGAGATTTGGCCCCTACTTA+TGG + chr5_4:45497916-45497935 Msa0853610:CDS 50.0%
TAGGGTCTGTGCGTTTCGTT+TGG - chr5_4:45496214-45496233 None:intergenic 50.0%
GAAAGATTACGGCGTACGGT+GGG - chr5_4:45496277-45496296 None:intergenic 50.0%
CCACGAAAGATTACGGCGTA+CGG - chr5_4:45496281-45496300 None:intergenic 50.0%
CTACAAACATTGGAGACGGC+GGG - chr5_4:45496335-45496354 None:intergenic 50.0%
CTGGTGGTTGTGAATAGAGC+TGG + chr5_4:45497012-45497031 Msa0853610:intron 50.0%
TTGTGAATAGAGCTGGCGCA+TGG + chr5_4:45497019-45497038 Msa0853610:intron 50.0%
CTACTGTGAAGGCTAGTGCT+TGG + chr5_4:45497642-45497661 Msa0853610:intron 50.0%
GGAGATTTGGCCCCTACTTA+TGG + chr5_4:45497916-45497935 Msa0853610:CDS 50.0%
CGAAAGATTACGGCGTACGG+TGG - chr5_4:45496278-45496297 None:intergenic 55.0%
CCGTACGCCGTAATCTTTCG+TGG + chr5_4:45496278-45496297 Msa0853610:five_prime_UTR 55.0%
AGAAACCTTGTGTCCGGAGG+TGG + chr5_4:45497677-45497696 Msa0853610:CDS 55.0%
CCTATCAGCAGTTCCACCTC+CGG - chr5_4:45497693-45497712 None:intergenic 55.0%
GCTCACAAGACACAAGACGG+TGG - chr5_4:45497745-45497764 None:intergenic 55.0%
!! GCCCCTACTTATGGTGTTGC+TGG + chr5_4:45497925-45497944 Msa0853610:CDS 55.0%
GCCCCTGCATATGATGTTGC+TGG + chr5_4:45497962-45497981 Msa0853610:three_prime_UTR 55.0%
CGAAAGATTACGGCGTACGG+TGG - chr5_4:45496278-45496297 None:intergenic 55.0%
CCGTACGCCGTAATCTTTCG+TGG + chr5_4:45496278-45496297 Msa0853610:five_prime_UTR 55.0%
AGAAACCTTGTGTCCGGAGG+TGG + chr5_4:45497677-45497696 Msa0853610:CDS 55.0%
CCTATCAGCAGTTCCACCTC+CGG - chr5_4:45497693-45497712 None:intergenic 55.0%
GCTCACAAGACACAAGACGG+TGG - chr5_4:45497745-45497764 None:intergenic 55.0%
!! GCCCCTACTTATGGTGTTGC+TGG + chr5_4:45497925-45497944 Msa0853610:CDS 55.0%
GCCCCTGCATATGATGTTGC+TGG + chr5_4:45497962-45497981 Msa0853610:three_prime_UTR 55.0%
GGTCGTCTCCCATCTTCGTG+AGG + chr5_4:45496299-45496318 Msa0853610:five_prime_UTR 60.0%
CAGTTCCACCTCCGGACACA+AGG - chr5_4:45497685-45497704 None:intergenic 60.0%
CCGGAGGTGGAACTGCTGAT+AGG + chr5_4:45497690-45497709 Msa0853610:CDS 60.0%
GGTCGTCTCCCATCTTCGTG+AGG + chr5_4:45496299-45496318 Msa0853610:five_prime_UTR 60.0%
CAGTTCCACCTCCGGACACA+AGG - chr5_4:45497685-45497704 None:intergenic 60.0%
CCGGAGGTGGAACTGCTGAT+AGG + chr5_4:45497690-45497709 Msa0853610:CDS 60.0%
TACGGCGTACGGTGGGAGAC+AGG - chr5_4:45496270-45496289 None:intergenic 65.0%
TACGGCGTACGGTGGGAGAC+AGG - chr5_4:45496270-45496289 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 45496212 45498407 45496212 ID=Msa0853610;Name=Msa0853610
chr5_4 mRNA 45496212 45498407 45496212 ID=Msa0853610-mRNA-1;Parent=Msa0853610;Name=Msa0853610-mRNA-1;_AED=0.15;_eAED=0.15;_QI=133|0.25|0.4|1|0.25|0.4|5|453|104
chr5_4 exon 45496212 45496400 45496212 ID=Msa0853610-mRNA-1:exon:14342;Parent=Msa0853610-mRNA-1
chr5_4 exon 45497078 45497147 45497078 ID=Msa0853610-mRNA-1:exon:14343;Parent=Msa0853610-mRNA-1
chr5_4 exon 45497586 45497591 45497586 ID=Msa0853610-mRNA-1:exon:14344;Parent=Msa0853610-mRNA-1
chr5_4 exon 45497653 45497784 45497653 ID=Msa0853610-mRNA-1:exon:14345;Parent=Msa0853610-mRNA-1
chr5_4 exon 45497904 45498407 45497904 ID=Msa0853610-mRNA-1:exon:14346;Parent=Msa0853610-mRNA-1
chr5_4 five_prime_UTR 45496212 45496344 45496212 ID=Msa0853610-mRNA-1:five_prime_utr;Parent=Msa0853610-mRNA-1
chr5_4 CDS 45496345 45496400 45496345 ID=Msa0853610-mRNA-1:cds;Parent=Msa0853610-mRNA-1
chr5_4 CDS 45497078 45497147 45497078 ID=Msa0853610-mRNA-1:cds;Parent=Msa0853610-mRNA-1
chr5_4 CDS 45497586 45497591 45497586 ID=Msa0853610-mRNA-1:cds;Parent=Msa0853610-mRNA-1
chr5_4 CDS 45497653 45497784 45497653 ID=Msa0853610-mRNA-1:cds;Parent=Msa0853610-mRNA-1
chr5_4 CDS 45497904 45497954 45497904 ID=Msa0853610-mRNA-1:cds;Parent=Msa0853610-mRNA-1
chr5_4 three_prime_UTR 45497955 45498407 45497955 ID=Msa0853610-mRNA-1:three_prime_utr;Parent=Msa0853610-mRNA-1
Gene Sequence

>Msa0853610

ATGTTTGTAGAAACTGAAAGGTTAATCGCCTCCCTCATCGTTGCTGAAAAACGCAGGAACATACTTCAACATGACATGAAGGTTTCAAGTCACTTTATTGGCAAGCTTGTTAGCCATAAGTTTGAGCTTTTGGCTAGTGCTTGGTCCCCTCACTATAGAAACCTTGTGTCCGGAGGTGGAACTGCTGATAGGTGTATTCTTTTCTGGAACACTACAAACATCCACCGTCTTGTGTCTTGTGAGCTACAAATGCTACATTGGGAGAGTCAAAACTTTGGAGATTTGGCCCCTACTTATGGTGTTGCTGGATTTTAA
Protein sequence

>Msa0853610

MFVETERLIASLIVAEKRRNILQHDMKVSSHFIGKLVSHKFELLASAWSPHYRNLVSGGGTADRCILFWNTTNIHRLVSCELQMLHWESQNFGDLAPTYGVAGF*