AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1288560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1288560 MtrunA17_Chr5g0393511 40.994 161 83 4 4 160 9 161 4.41e-38 127
Msa1288560 MtrunA17_Chr8g0359031 43.750 144 72 3 25 160 19 161 1.97e-36 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTGCTATGTTAAATTTC+TGG 0.153996 8_3:+41609485 Msa1288560:CDS
TGGGAGGTGCTGATTCATTC+TGG 0.307310 8_3:+41609112 Msa1288560:CDS
CTTCTCTGCATCAACCTTAC+TGG 0.321088 8_3:-41608969 None:intergenic
GTTTAAGATGGACAGTAATC+CGG 0.386700 8_3:+41609461 Msa1288560:CDS
TACATTTGTTGAAGCGAGTA+AGG 0.420870 8_3:+41609416 Msa1288560:CDS
AGACATGGAGGAACCGGTAT+TGG 0.457153 8_3:-41609070 None:intergenic
TGAATGTTCGAACAAATTCA+TGG 0.458298 8_3:+41609092 Msa1288560:CDS
CGAATTCCATTCCACATCAA+TGG 0.458515 8_3:+41609252 Msa1288560:CDS
GCAGGTTCTACATCAATTTG+AGG 0.461169 8_3:-41609031 None:intergenic
TGACAGATTGTTGTTTAAGA+TGG 0.463808 8_3:+41609449 Msa1288560:CDS
ACATTCAGACATGGAGGAAC+CGG 0.479236 8_3:-41609076 None:intergenic
AATGTCAGAGTCTCTTTCCC+AGG 0.481300 8_3:-41609288 None:intergenic
TTTAAGATGGACAGTAATCC+GGG 0.497303 8_3:+41609462 Msa1288560:CDS
AAAGATCCATTGATGTGGAA+TGG 0.510519 8_3:-41609258 None:intergenic
TATGTGCAGAAGAAATTCAT+AGG 0.511115 8_3:+41609381 Msa1288560:CDS
ATGCAATATTCGAATGAAGA+AGG 0.515264 8_3:+41608992 Msa1288560:CDS
GGCTCACGGTAATCATCTGC+AGG 0.521858 8_3:-41609049 None:intergenic
AAGATGCAGTTGATGCGATC+TGG 0.525388 8_3:+41609331 Msa1288560:CDS
TGCAGAAGAAATTCATAGGA+AGG 0.530136 8_3:+41609385 Msa1288560:CDS
TAGATTACCAGCTTATGAGT+TGG 0.531267 8_3:-41609145 None:intergenic
TTCTTCTGCACATAATCACC+AGG 0.548008 8_3:-41609372 None:intergenic
TTTACGTCCAACTCATAAGC+TGG 0.553121 8_3:+41609138 Msa1288560:CDS
CAGAAGAAATTCATAGGAAG+GGG 0.562018 8_3:+41609387 Msa1288560:CDS
TGATTACCGTGAGCCAATAC+CGG 0.564459 8_3:+41609057 Msa1288560:CDS
AGAAATTCATAGGAAGGGGT+TGG 0.574152 8_3:+41609391 Msa1288560:CDS
GAAGCGAGTAAGGTGAAAGT+TGG 0.578483 8_3:+41609426 Msa1288560:CDS
GCAGAAGAAATTCATAGGAA+GGG 0.580030 8_3:+41609386 Msa1288560:CDS
GAATGTTCGAACAAATTCAT+GGG 0.580892 8_3:+41609093 Msa1288560:CDS
AGAGACTCTGACATTGACAT+TGG 0.584037 8_3:+41609296 Msa1288560:CDS
TTACTGCAGTGAGTCCAGTA+AGG 0.589344 8_3:+41608955 Msa1288560:intron
AATTTAACATAGCAATTACC+CGG 0.590991 8_3:-41609480 None:intergenic
CACGAAAAGATCCATTGATG+TGG 0.608405 8_3:-41609263 None:intergenic
TTTGTTCGAACATTCAGACA+TGG 0.613761 8_3:-41609085 None:intergenic
TGAGAATGGCAACATTGAAG+AGG 0.623240 8_3:+41608858 None:intergenic
GAGGAACCGGTATTGGCTCA+CGG 0.632876 8_3:-41609063 None:intergenic
CAATATTCGAATGAAGAAGG+AGG 0.651295 8_3:+41608995 Msa1288560:CDS
TGTGATATTGATGTTGAACC+TGG 0.654348 8_3:+41609354 Msa1288560:CDS
GTTCGAACATTCAGACATGG+AGG 0.688432 8_3:-41609082 None:intergenic
TGTTCGAACAAATTCATGGG+AGG 0.689256 8_3:+41609096 Msa1288560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAACTAACATAAAGTAA+GGG - chr8_3:41609179-41609198 None:intergenic 15.0%
!! TATAAAACTAACATAAAGTA+AGG - chr8_3:41609180-41609199 None:intergenic 15.0%
!! TAATTGCTATGTTAAATTTC+TGG + chr8_3:41609485-41609504 Msa1288560:CDS 20.0%
! TGAAGAGGTAATAAAGTTTA+TGG + chr8_3:41608873-41608892 Msa1288560:intron 25.0%
! AGTATCAAGTCAACATAAAA+TGG - chr8_3:41608926-41608945 None:intergenic 25.0%
! AATTTAACATAGCAATTACC+CGG - chr8_3:41609483-41609502 None:intergenic 25.0%
ATGCAATATTCGAATGAAGA+AGG + chr8_3:41608992-41609011 Msa1288560:CDS 30.0%
TGAATGTTCGAACAAATTCA+TGG + chr8_3:41609092-41609111 Msa1288560:CDS 30.0%
GAATGTTCGAACAAATTCAT+GGG + chr8_3:41609093-41609112 Msa1288560:CDS 30.0%
TATGTGCAGAAGAAATTCAT+AGG + chr8_3:41609381-41609400 Msa1288560:CDS 30.0%
!! TGACAGATTGTTGTTTAAGA+TGG + chr8_3:41609449-41609468 Msa1288560:CDS 30.0%
CAATATTCGAATGAAGAAGG+AGG + chr8_3:41608995-41609014 Msa1288560:CDS 35.0%
TTTGTTCGAACATTCAGACA+TGG - chr8_3:41609088-41609107 None:intergenic 35.0%
TAGATTACCAGCTTATGAGT+TGG - chr8_3:41609148-41609167 None:intergenic 35.0%
AAAGATCCATTGATGTGGAA+TGG - chr8_3:41609261-41609280 None:intergenic 35.0%
TGTGATATTGATGTTGAACC+TGG + chr8_3:41609354-41609373 Msa1288560:CDS 35.0%
TGCAGAAGAAATTCATAGGA+AGG + chr8_3:41609385-41609404 Msa1288560:CDS 35.0%
GCAGAAGAAATTCATAGGAA+GGG + chr8_3:41609386-41609405 Msa1288560:CDS 35.0%
CAGAAGAAATTCATAGGAAG+GGG + chr8_3:41609387-41609406 Msa1288560:CDS 35.0%
! TACATTTGTTGAAGCGAGTA+AGG + chr8_3:41609416-41609435 Msa1288560:CDS 35.0%
! GTTTAAGATGGACAGTAATC+CGG + chr8_3:41609461-41609480 Msa1288560:CDS 35.0%
! TTTAAGATGGACAGTAATCC+GGG + chr8_3:41609462-41609481 Msa1288560:CDS 35.0%
GCAGGTTCTACATCAATTTG+AGG - chr8_3:41609034-41609053 None:intergenic 40.0%
TGTTCGAACAAATTCATGGG+AGG + chr8_3:41609096-41609115 Msa1288560:CDS 40.0%
TTTACGTCCAACTCATAAGC+TGG + chr8_3:41609138-41609157 Msa1288560:CDS 40.0%
CGAATTCCATTCCACATCAA+TGG + chr8_3:41609252-41609271 Msa1288560:CDS 40.0%
CACGAAAAGATCCATTGATG+TGG - chr8_3:41609266-41609285 None:intergenic 40.0%
! AATGGATCTTTTCGTGAACC+TGG + chr8_3:41609270-41609289 Msa1288560:CDS 40.0%
! ATGGATCTTTTCGTGAACCT+GGG + chr8_3:41609271-41609290 Msa1288560:CDS 40.0%
AGAGACTCTGACATTGACAT+TGG + chr8_3:41609296-41609315 Msa1288560:CDS 40.0%
TTCTTCTGCACATAATCACC+AGG - chr8_3:41609375-41609394 None:intergenic 40.0%
AGAAATTCATAGGAAGGGGT+TGG + chr8_3:41609391-41609410 Msa1288560:CDS 40.0%
TTACTGCAGTGAGTCCAGTA+AGG + chr8_3:41608955-41608974 Msa1288560:intron 45.0%
CTTCTCTGCATCAACCTTAC+TGG - chr8_3:41608972-41608991 None:intergenic 45.0%
TGATTACCGTGAGCCAATAC+CGG + chr8_3:41609057-41609076 Msa1288560:CDS 45.0%
ACATTCAGACATGGAGGAAC+CGG - chr8_3:41609079-41609098 None:intergenic 45.0%
GTTCGAACATTCAGACATGG+AGG - chr8_3:41609085-41609104 None:intergenic 45.0%
AATGTCAGAGTCTCTTTCCC+AGG - chr8_3:41609291-41609310 None:intergenic 45.0%
! AAGATGCAGTTGATGCGATC+TGG + chr8_3:41609331-41609350 Msa1288560:CDS 45.0%
GAAGCGAGTAAGGTGAAAGT+TGG + chr8_3:41609426-41609445 Msa1288560:CDS 45.0%
AGACATGGAGGAACCGGTAT+TGG - chr8_3:41609073-41609092 None:intergenic 50.0%
!! TGGGAGGTGCTGATTCATTC+TGG + chr8_3:41609112-41609131 Msa1288560:CDS 50.0%
GGCTCACGGTAATCATCTGC+AGG - chr8_3:41609052-41609071 None:intergenic 55.0%
!! GAGGAACCGGTATTGGCTCA+CGG - chr8_3:41609066-41609085 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 41608863 41609521 41608863 ID=Msa1288560;Name=Msa1288560
chr8_3 mRNA 41608863 41609521 41608863 ID=Msa1288560-mRNA-1;Parent=Msa1288560;Name=Msa1288560-mRNA-1;_AED=0.09;_eAED=0.10;_QI=0|0|0|0.66|1|1|3|0|160
chr8_3 exon 41608863 41608879 41608863 ID=Msa1288560-mRNA-1:exon:11410;Parent=Msa1288560-mRNA-1
chr8_3 exon 41608964 41609159 41608964 ID=Msa1288560-mRNA-1:exon:11411;Parent=Msa1288560-mRNA-1
chr8_3 exon 41609252 41609521 41609252 ID=Msa1288560-mRNA-1:exon:11412;Parent=Msa1288560-mRNA-1
chr8_3 CDS 41608863 41608879 41608863 ID=Msa1288560-mRNA-1:cds;Parent=Msa1288560-mRNA-1
chr8_3 CDS 41608964 41609159 41608964 ID=Msa1288560-mRNA-1:cds;Parent=Msa1288560-mRNA-1
chr8_3 CDS 41609252 41609521 41609252 ID=Msa1288560-mRNA-1:cds;Parent=Msa1288560-mRNA-1
Gene Sequence

>Msa1288560

ATGGCAACATTGAAGAGTGAGTCCAGTAAGGTTGATGCAGAGAAGATGCAATATTCGAATGAAGAAGGAGGAAGATCTGCAGAACCTCAAATTGATGTAGAACCTGCAGATGATTACCGTGAGCCAATACCGGTTCCTCCATGTCTGAATGTTCGAACAAATTCATGGGAGGTGCTGATTCATTCTGGTCATTTACGTCCAACTCATAAGCTGCGAATTCCATTCCACATCAATGGATCTTTTCGTGAACCTGGGAAAGAGACTCTGACATTGACATTGGTTGATTATGAAGAAGATGCAGTTGATGCGATCTGGTGTGATATTGATGTTGAACCTGGTGATTATGTGCAGAAGAAATTCATAGGAAGGGGTTGGCATACATTTGTTGAAGCGAGTAAGGTGAAAGTTGGTGACAGATTGTTGTTTAAGATGGACAGTAATCCGGGTAATTGCTATGTTAAATTTCTGGAAAGTGATATATAA

Protein sequence

>Msa1288560

MATLKSESSKVDAEKMQYSNEEGGRSAEPQIDVEPADDYREPIPVPPCLNVRTNSWEVLIHSGHLRPTHKLRIPFHINGSFREPGKETLTLTLVDYEEDAVDAIWCDIDVEPGDYVQKKFIGRGWHTFVEASKVKVGDRLLFKMDSNPGNCYVKFLESDI*