AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0868200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868200 MtrunA17_Chr3g0080411 97.846 325 5 1 1 323 1 325 0.0 661
Msa0868200 MtrunA17_Chr3g0108541 35.185 324 177 14 7 318 8 310 3.10e-39 140
Msa0868200 MtrunA17_Chr5g0432911 33.750 320 188 12 1 318 4 301 6.49e-39 139
Msa0868200 MtrunA17_Chr8g0358861 29.560 318 193 10 8 318 1 294 1.17e-29 114
Msa0868200 MtrunA17_Chr7g0245311 29.283 321 197 10 7 317 13 313 1.16e-27 109
Msa0868200 MtrunA17_Chr8g0347761 27.817 284 176 10 37 314 34 294 4.76e-22 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868200 AT4G22900.1 46.471 340 151 9 1 317 1 332 1.31e-96 290
Msa0868200 AT4G11950.1 44.172 326 151 10 14 317 10 326 1.81e-82 253
Msa0868200 AT1G62981.2 42.071 309 153 12 28 318 41 341 1.06e-62 202
Msa0868200 AT1G62981.1 42.071 309 153 12 28 318 41 341 1.06e-62 202
Msa0868200 AT3G08600.1 32.550 298 161 13 33 313 37 311 1.19e-22 96.7
Msa0868200 AT4G23720.1 27.055 292 186 12 26 313 38 306 1.38e-18 85.1
Msa0868200 AT4G01140.1 27.986 293 185 13 27 316 28 297 1.18e-16 79.7
Msa0868200 AT1G62981.3 35.135 185 103 8 146 318 29 208 7.25e-16 75.5

Find 43 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAAAACTCATGGACATTT+TGG 0.170821 6_1:+1717667 Msa0868200:CDS
TTCCATTGACTTAGTCTTAT+CGG 0.242691 6_1:-1717736 None:intergenic
AGTACTATTGGTGCTGCTTT+AGG 0.264423 6_1:+1717772 Msa0868200:CDS
CCTTCAAAACTTACACATAA+AGG 0.290832 6_1:-1717589 None:intergenic
TTATTGTCATTGAAATTTCA+TGG 0.299770 6_1:+1717067 Msa0868200:CDS
AAGCTACCTTCAAATTTATC+AGG 0.315618 6_1:+1717199 Msa0868200:CDS
CAACTTGTTTCTCCAATTGT+TGG 0.336972 6_1:+1717418 Msa0868200:CDS
TAAGCTAAAATTCCAACAAT+TGG 0.344113 6_1:-1717430 None:intergenic
GTTGGTGTTGTTCTAGTTCC+AGG 0.353242 6_1:-1717937 None:intergenic
TTATGAAGAAGAAGCACTTC+AGG 0.366104 6_1:+1717879 Msa0868200:CDS
GCATAGAGTATAATAATAAT+TGG 0.373049 6_1:+1717353 Msa0868200:CDS
AAGGTATGGTGCAAAATTAA+AGG 0.383254 6_1:+1717264 Msa0868200:CDS
AAGGAGTTTCATCTTGGTAT+TGG 0.399934 6_1:+1717283 Msa0868200:CDS
ATTGCAGTTGGGAGTACTAT+TGG 0.400361 6_1:+1717760 Msa0868200:CDS
TTTCAAGAATCAAGAAGAAA+AGG 0.407881 6_1:+1717552 Msa0868200:CDS
TTGATGCCTGATAAATTTGA+AGG 0.417038 6_1:-1717205 None:intergenic
GCTTCAATTCCAAGTTGAAA+AGG 0.422597 6_1:-1717487 None:intergenic
ACACAAACATTTGTTGCTAA+AGG 0.423104 6_1:-1717643 None:intergenic
GCACTTCAGGTTTCAATGGT+TGG 0.434477 6_1:+1717892 Msa0868200:CDS
AGAAGCACTTCAGGTTTCAA+TGG 0.442190 6_1:+1717888 Msa0868200:CDS
GAAGGTAGCTTCACAGATTG+AGG 0.451958 6_1:-1717187 None:intergenic
CTTCAATTCCAAGTTGAAAA+GGG 0.452206 6_1:-1717486 None:intergenic
AAATTAAAGGAGTTTCATCT+TGG 0.452708 6_1:+1717277 Msa0868200:CDS
AACATTACTCTTTCAATACA+TGG 0.463603 6_1:-1717319 None:intergenic
TTTCAACTTGGAATTGAAGC+TGG 0.480245 6_1:+1717490 Msa0868200:CDS
TTAGACAAAGATTTCAAGAC+AGG 0.484697 6_1:+1717160 Msa0868200:CDS
GTTCTAGTTCCAGGTGCAGT+AGG 0.488529 6_1:-1717928 None:intergenic
GTCAATGGAAAATTGCAGTT+GGG 0.491640 6_1:+1717749 Msa0868200:CDS
GGTTAAGAAAAGGTCAAGAA+TGG 0.493692 6_1:+1717837 Msa0868200:CDS
AGCCGATAAGACTAAGTCAA+TGG 0.499042 6_1:+1717734 Msa0868200:CDS
GTTTGTGTTGCTAAAACTCA+TGG 0.503467 6_1:+1717658 Msa0868200:CDS
TGTGGAAGTTTAACAAGGTA+TGG 0.509899 6_1:+1717250 Msa0868200:CDS
AGTCAATGGAAAATTGCAGT+TGG 0.516447 6_1:+1717748 Msa0868200:CDS
TTCAAGAATCAAGAAGAAAA+GGG 0.519947 6_1:+1717553 Msa0868200:CDS
AAGGTCAAGAATGGTTCAGA+TGG 0.524303 6_1:+1717846 Msa0868200:CDS
TGAATGTGTGATCTCTTATG+AGG 0.547320 6_1:-1717132 None:intergenic
TGGTTGTTAGGGTTAAGAAA+AGG 0.555158 6_1:+1717827 Msa0868200:CDS
GTGAGAGCTCCTACTGCACC+TGG 0.585262 6_1:+1717919 Msa0868200:CDS
GATACTGTTAGATTTAGATG+TGG 0.588960 6_1:+1717232 Msa0868200:CDS
TTAGATGTGGAAGTTTAACA+AGG 0.623871 6_1:+1717245 Msa0868200:CDS
TAGTCATGTTGCTCAAGTGT+TGG 0.655160 6_1:-1717955 None:intergenic
AGTAAACCTCGTCATCAACG+AGG 0.739646 6_1:-1717991 None:intergenic
ATGTGTGATCTCTTATGAGG+AGG 0.743160 6_1:-1717129 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTGTCATTGAAATTTCA+TGG + chr6_1:1717067-1717086 Msa0868200:CDS 20.0%
!! GCATAGAGTATAATAATAAT+TGG + chr6_1:1717353-1717372 Msa0868200:CDS 20.0%
!! TTATTGTCATTGAAATTTCA+TGG + chr6_1:1717067-1717086 Msa0868200:CDS 20.0%
!! GCATAGAGTATAATAATAAT+TGG + chr6_1:1717353-1717372 Msa0868200:CDS 20.0%
! AAATTAAAGGAGTTTCATCT+TGG + chr6_1:1717277-1717296 Msa0868200:CDS 25.0%
! AACATTACTCTTTCAATACA+TGG - chr6_1:1717322-1717341 None:intergenic 25.0%
! TAAGCTAAAATTCCAACAAT+TGG - chr6_1:1717433-1717452 None:intergenic 25.0%
! TTTCAAGAATCAAGAAGAAA+AGG + chr6_1:1717552-1717571 Msa0868200:CDS 25.0%
! TTCAAGAATCAAGAAGAAAA+GGG + chr6_1:1717553-1717572 Msa0868200:CDS 25.0%
! AAATTAAAGGAGTTTCATCT+TGG + chr6_1:1717277-1717296 Msa0868200:CDS 25.0%
! AACATTACTCTTTCAATACA+TGG - chr6_1:1717322-1717341 None:intergenic 25.0%
! TAAGCTAAAATTCCAACAAT+TGG - chr6_1:1717433-1717452 None:intergenic 25.0%
! TTTCAAGAATCAAGAAGAAA+AGG + chr6_1:1717552-1717571 Msa0868200:CDS 25.0%
! TTCAAGAATCAAGAAGAAAA+GGG + chr6_1:1717553-1717572 Msa0868200:CDS 25.0%
TTAGACAAAGATTTCAAGAC+AGG + chr6_1:1717160-1717179 Msa0868200:CDS 30.0%
AAGCTACCTTCAAATTTATC+AGG + chr6_1:1717199-1717218 Msa0868200:CDS 30.0%
TTGATGCCTGATAAATTTGA+AGG - chr6_1:1717208-1717227 None:intergenic 30.0%
GATACTGTTAGATTTAGATG+TGG + chr6_1:1717232-1717251 Msa0868200:CDS 30.0%
TTAGATGTGGAAGTTTAACA+AGG + chr6_1:1717245-1717264 Msa0868200:CDS 30.0%
AAGGTATGGTGCAAAATTAA+AGG + chr6_1:1717264-1717283 Msa0868200:CDS 30.0%
! CTTCAATTCCAAGTTGAAAA+GGG - chr6_1:1717489-1717508 None:intergenic 30.0%
CCTTCAAAACTTACACATAA+AGG - chr6_1:1717592-1717611 None:intergenic 30.0%
!! CCTTTATGTGTAAGTTTTGA+AGG + chr6_1:1717589-1717608 Msa0868200:CDS 30.0%
!!! TGTGTAAGTTTTGAAGGAAA+TGG + chr6_1:1717595-1717614 Msa0868200:CDS 30.0%
! ACACAAACATTTGTTGCTAA+AGG - chr6_1:1717646-1717665 None:intergenic 30.0%
!! TTCCATTGACTTAGTCTTAT+CGG - chr6_1:1717739-1717758 None:intergenic 30.0%
!!! AGGTTTACTTTTTGTAGCAA+TGG + chr6_1:1717807-1717826 Msa0868200:CDS 30.0%
! TTTTTGTAGCAATGGTTGTT+AGG + chr6_1:1717815-1717834 Msa0868200:CDS 30.0%
! TTTTGTAGCAATGGTTGTTA+GGG + chr6_1:1717816-1717835 Msa0868200:CDS 30.0%
TTAGACAAAGATTTCAAGAC+AGG + chr6_1:1717160-1717179 Msa0868200:CDS 30.0%
AAGCTACCTTCAAATTTATC+AGG + chr6_1:1717199-1717218 Msa0868200:CDS 30.0%
TTGATGCCTGATAAATTTGA+AGG - chr6_1:1717208-1717227 None:intergenic 30.0%
GATACTGTTAGATTTAGATG+TGG + chr6_1:1717232-1717251 Msa0868200:CDS 30.0%
TTAGATGTGGAAGTTTAACA+AGG + chr6_1:1717245-1717264 Msa0868200:CDS 30.0%
AAGGTATGGTGCAAAATTAA+AGG + chr6_1:1717264-1717283 Msa0868200:CDS 30.0%
! CTTCAATTCCAAGTTGAAAA+GGG - chr6_1:1717489-1717508 None:intergenic 30.0%
CCTTCAAAACTTACACATAA+AGG - chr6_1:1717592-1717611 None:intergenic 30.0%
!! CCTTTATGTGTAAGTTTTGA+AGG + chr6_1:1717589-1717608 Msa0868200:CDS 30.0%
!!! TGTGTAAGTTTTGAAGGAAA+TGG + chr6_1:1717595-1717614 Msa0868200:CDS 30.0%
! ACACAAACATTTGTTGCTAA+AGG - chr6_1:1717646-1717665 None:intergenic 30.0%
!! TTCCATTGACTTAGTCTTAT+CGG - chr6_1:1717739-1717758 None:intergenic 30.0%
!!! AGGTTTACTTTTTGTAGCAA+TGG + chr6_1:1717807-1717826 Msa0868200:CDS 30.0%
! TTTTTGTAGCAATGGTTGTT+AGG + chr6_1:1717815-1717834 Msa0868200:CDS 30.0%
! TTTTGTAGCAATGGTTGTTA+GGG + chr6_1:1717816-1717835 Msa0868200:CDS 30.0%
TGAATGTGTGATCTCTTATG+AGG - chr6_1:1717135-1717154 None:intergenic 35.0%
TGTGGAAGTTTAACAAGGTA+TGG + chr6_1:1717250-1717269 Msa0868200:CDS 35.0%
AAGGAGTTTCATCTTGGTAT+TGG + chr6_1:1717283-1717302 Msa0868200:CDS 35.0%
! CAACTTGTTTCTCCAATTGT+TGG + chr6_1:1717418-1717437 Msa0868200:CDS 35.0%
! AGTAGTAACCCTTTTCAACT+TGG + chr6_1:1717478-1717497 Msa0868200:CDS 35.0%
! GCTTCAATTCCAAGTTGAAA+AGG - chr6_1:1717490-1717509 None:intergenic 35.0%
TTTCAACTTGGAATTGAAGC+TGG + chr6_1:1717490-1717509 Msa0868200:CDS 35.0%
! GTTTGTGTTGCTAAAACTCA+TGG + chr6_1:1717658-1717677 Msa0868200:CDS 35.0%
! GCTAAAACTCATGGACATTT+TGG + chr6_1:1717667-1717686 Msa0868200:CDS 35.0%
!!! AACTCATGGACATTTTGGTT+TGG + chr6_1:1717672-1717691 Msa0868200:CDS 35.0%
AGTCAATGGAAAATTGCAGT+TGG + chr6_1:1717748-1717767 Msa0868200:CDS 35.0%
GTCAATGGAAAATTGCAGTT+GGG + chr6_1:1717749-1717768 Msa0868200:CDS 35.0%
!! GCTTTAGGTGCTTTTCTTTT+AGG + chr6_1:1717787-1717806 Msa0868200:CDS 35.0%
TGGTTGTTAGGGTTAAGAAA+AGG + chr6_1:1717827-1717846 Msa0868200:CDS 35.0%
GGTTAAGAAAAGGTCAAGAA+TGG + chr6_1:1717837-1717856 Msa0868200:CDS 35.0%
!! TTATGAAGAAGAAGCACTTC+AGG + chr6_1:1717879-1717898 Msa0868200:CDS 35.0%
TGAATGTGTGATCTCTTATG+AGG - chr6_1:1717135-1717154 None:intergenic 35.0%
TGTGGAAGTTTAACAAGGTA+TGG + chr6_1:1717250-1717269 Msa0868200:CDS 35.0%
AAGGAGTTTCATCTTGGTAT+TGG + chr6_1:1717283-1717302 Msa0868200:CDS 35.0%
! CAACTTGTTTCTCCAATTGT+TGG + chr6_1:1717418-1717437 Msa0868200:CDS 35.0%
! AGTAGTAACCCTTTTCAACT+TGG + chr6_1:1717478-1717497 Msa0868200:CDS 35.0%
! GCTTCAATTCCAAGTTGAAA+AGG - chr6_1:1717490-1717509 None:intergenic 35.0%
TTTCAACTTGGAATTGAAGC+TGG + chr6_1:1717490-1717509 Msa0868200:CDS 35.0%
! GTTTGTGTTGCTAAAACTCA+TGG + chr6_1:1717658-1717677 Msa0868200:CDS 35.0%
! GCTAAAACTCATGGACATTT+TGG + chr6_1:1717667-1717686 Msa0868200:CDS 35.0%
!!! AACTCATGGACATTTTGGTT+TGG + chr6_1:1717672-1717691 Msa0868200:CDS 35.0%
AGTCAATGGAAAATTGCAGT+TGG + chr6_1:1717748-1717767 Msa0868200:CDS 35.0%
GTCAATGGAAAATTGCAGTT+GGG + chr6_1:1717749-1717768 Msa0868200:CDS 35.0%
!! GCTTTAGGTGCTTTTCTTTT+AGG + chr6_1:1717787-1717806 Msa0868200:CDS 35.0%
TGGTTGTTAGGGTTAAGAAA+AGG + chr6_1:1717827-1717846 Msa0868200:CDS 35.0%
GGTTAAGAAAAGGTCAAGAA+TGG + chr6_1:1717837-1717856 Msa0868200:CDS 35.0%
!! TTATGAAGAAGAAGCACTTC+AGG + chr6_1:1717879-1717898 Msa0868200:CDS 35.0%
ATGTGTGATCTCTTATGAGG+AGG - chr6_1:1717132-1717151 None:intergenic 40.0%
AGCCGATAAGACTAAGTCAA+TGG + chr6_1:1717734-1717753 Msa0868200:CDS 40.0%
!! ATTGCAGTTGGGAGTACTAT+TGG + chr6_1:1717760-1717779 Msa0868200:CDS 40.0%
!! AGTACTATTGGTGCTGCTTT+AGG + chr6_1:1717772-1717791 Msa0868200:CDS 40.0%
AAGGTCAAGAATGGTTCAGA+TGG + chr6_1:1717846-1717865 Msa0868200:CDS 40.0%
!! AGAAGCACTTCAGGTTTCAA+TGG + chr6_1:1717888-1717907 Msa0868200:CDS 40.0%
TAGTCATGTTGCTCAAGTGT+TGG - chr6_1:1717958-1717977 None:intergenic 40.0%
ATGTGTGATCTCTTATGAGG+AGG - chr6_1:1717132-1717151 None:intergenic 40.0%
AGCCGATAAGACTAAGTCAA+TGG + chr6_1:1717734-1717753 Msa0868200:CDS 40.0%
!! ATTGCAGTTGGGAGTACTAT+TGG + chr6_1:1717760-1717779 Msa0868200:CDS 40.0%
!! AGTACTATTGGTGCTGCTTT+AGG + chr6_1:1717772-1717791 Msa0868200:CDS 40.0%
AAGGTCAAGAATGGTTCAGA+TGG + chr6_1:1717846-1717865 Msa0868200:CDS 40.0%
!! AGAAGCACTTCAGGTTTCAA+TGG + chr6_1:1717888-1717907 Msa0868200:CDS 40.0%
TAGTCATGTTGCTCAAGTGT+TGG - chr6_1:1717958-1717977 None:intergenic 40.0%
GAAGGTAGCTTCACAGATTG+AGG - chr6_1:1717190-1717209 None:intergenic 45.0%
! GCACTTCAGGTTTCAATGGT+TGG + chr6_1:1717892-1717911 Msa0868200:CDS 45.0%
!! GTTGGTGTTGTTCTAGTTCC+AGG - chr6_1:1717940-1717959 None:intergenic 45.0%
GAAGGTAGCTTCACAGATTG+AGG - chr6_1:1717190-1717209 None:intergenic 45.0%
! GCACTTCAGGTTTCAATGGT+TGG + chr6_1:1717892-1717911 Msa0868200:CDS 45.0%
!! GTTGGTGTTGTTCTAGTTCC+AGG - chr6_1:1717940-1717959 None:intergenic 45.0%
!! GTTCTAGTTCCAGGTGCAGT+AGG - chr6_1:1717931-1717950 None:intergenic 50.0%
!! GTTCTAGTTCCAGGTGCAGT+AGG - chr6_1:1717931-1717950 None:intergenic 50.0%
GTGAGAGCTCCTACTGCACC+TGG + chr6_1:1717919-1717938 Msa0868200:CDS 60.0%
GTGAGAGCTCCTACTGCACC+TGG + chr6_1:1717919-1717938 Msa0868200:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 1717028 1717999 1717028 ID=Msa0868200;Name=Msa0868200
chr6_1 mRNA 1717028 1717999 1717028 ID=Msa0868200-mRNA-1;Parent=Msa0868200;Name=Msa0868200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|323
chr6_1 exon 1717028 1717999 1717028 ID=Msa0868200-mRNA-1:exon:445;Parent=Msa0868200-mRNA-1
chr6_1 CDS 1717028 1717999 1717028 ID=Msa0868200-mRNA-1:cds;Parent=Msa0868200-mRNA-1
Gene Sequence

>Msa0868200

ATGGACTCATTTTACACTCTTATGTTGATGACACTTTCATTATTGTCATTGAAATTTCATGGATCTTATGCTCAGAATATCAAATCAGTTCATGTTCTTGACCTCCTCATAAGAGATCACACATTCAAGTCATTAGACAAAGATTTCAAGACAGGAATACCTCAATCTGTGAAGCTACCTTCAAATTTATCAGGCATCAAAGTAGATACTGTTAGATTTAGATGTGGAAGTTTAACAAGGTATGGTGCAAAATTAAAGGAGTTTCATCTTGGTATTGGTGTTTCTATTCATCCATGTATTGAAAGAGTAATGTTGATAAGACAAAGCATAGAGTATAATAATAATTGGTCTTCTATATATTATGCAAACTATAATGATCTATCTAAGTATCAACTTGTTTCTCCAATTGTTGGAATTTTAGCTTACAATGCTGATGAAGATTCAAATTCTAGTAGTAACCCTTTTCAACTTGGAATTGAAGCTGGTGAAAAACTCATCACAATTGATTTCAACAATGCTACAAATTTCAAGAATCAAGAAGAAAAGGGTATCAAAGTAAAACCTTTATGTGTAAGTTTTGAAGGAAATGGAAAAATGACACTTGCAATAACAAAACCTTTAGCAACAAATGTTTGTGTTGCTAAAACTCATGGACATTTTGGTTTGGTTGTTGAGTCTTTACAACATCAAGATGATGAGTATAAAAAGCCGATAAGACTAAGTCAATGGAAAATTGCAGTTGGGAGTACTATTGGTGCTGCTTTAGGTGCTTTTCTTTTAGGTTTACTTTTTGTAGCAATGGTTGTTAGGGTTAAGAAAAGGTCAAGAATGGTTCAGATGGAAAGAAGAGCTTATGAAGAAGAAGCACTTCAGGTTTCAATGGTTGGACATGTGAGAGCTCCTACTGCACCTGGAACTAGAACAACACCAACACTTGAGCAACATGACTATATAACTCGACATCCTCGTTGA

Protein sequence

>Msa0868200

MDSFYTLMLMTLSLLSLKFHGSYAQNIKSVHVLDLLIRDHTFKSLDKDFKTGIPQSVKLPSNLSGIKVDTVRFRCGSLTRYGAKLKEFHLGIGVSIHPCIERVMLIRQSIEYNNNWSSIYYANYNDLSKYQLVSPIVGILAYNADEDSNSSSNPFQLGIEAGEKLITIDFNNATNFKNQEEKGIKVKPLCVSFEGNGKMTLAITKPLATNVCVAKTHGHFGLVVESLQHQDDEYKKPIRLSQWKIAVGSTIGAALGAFLLGLLFVAMVVRVKKRSRMVQMERRAYEEEALQVSMVGHVRAPTAPGTRTTPTLEQHDYITRHPR*