AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300320 MtrunA17_Chr2g0325101 92.737 179 0 1 1 166 1 179 1.06e-118 333
Msa0300320 MtrunA17_Chr4g0037021 82.682 179 18 1 1 166 1 179 2.28e-108 307
Msa0300320 MtrunA17_Chr8g0371501 80.337 178 22 1 1 165 1 178 1.26e-104 298
Msa0300320 MtrunA17_Chr5g0405791 79.213 178 24 1 1 165 1 178 2.55e-103 294
Msa0300320 MtrunA17_Chr6g0486041 76.536 179 29 1 1 166 1 179 3.92e-100 286
Msa0300320 MtrunA17_Chr3g0117931 74.719 178 32 1 2 166 4 181 3.05e-98 282
Msa0300320 MtrunA17_Chr4g0046211 85.915 142 7 1 1 129 1 142 4.26e-85 246
Msa0300320 MtrunA17_Chr7g0265001 33.735 166 103 4 8 167 18 182 1.49e-27 102
Msa0300320 MtrunA17_Chr1g0212521 34.177 158 96 5 6 157 15 170 2.82e-25 96.7
Msa0300320 MtrunA17_Chr8g0384311 32.530 166 104 5 6 165 13 176 4.07e-25 96.3
Msa0300320 MtrunA17_Chr1g0182321 34.177 158 96 5 6 157 15 170 5.13e-25 95.9
Msa0300320 MtrunA17_Chr4g0030911 34.177 158 96 5 6 157 15 170 6.27e-25 95.9
Msa0300320 MtrunA17_Chr1g0182301 34.177 158 96 5 6 157 15 170 9.22e-25 95.5
Msa0300320 MtrunA17_Chr7g0263071 33.544 158 97 5 6 157 15 170 2.33e-24 94.4
Msa0300320 MtrunA17_Chr2g0290891 32.099 162 103 5 1 157 1 160 4.84e-24 93.6
Msa0300320 MtrunA17_Chr6g0458081 30.994 171 111 5 1 166 1 169 6.88e-24 92.8
Msa0300320 MtrunA17_Chr3g0130271 31.325 166 105 5 7 165 13 176 7.72e-24 92.8
Msa0300320 MtrunA17_Chr1g0159481 31.928 166 105 5 6 165 17 180 3.69e-23 91.3
Msa0300320 MtrunA17_Chr1g0180291 32.927 164 101 6 8 164 16 177 8.11e-23 90.1
Msa0300320 MtrunA17_Chr8g0349711 29.630 162 107 5 1 157 1 160 6.84e-22 87.8
Msa0300320 MtrunA17_Chr8g0335471 31.847 157 100 5 6 157 8 162 9.04e-22 87.4
Msa0300320 MtrunA17_Chr4g0055081 34.839 155 94 5 8 157 11 163 1.82e-21 86.7
Msa0300320 MtrunA17_Chr7g0250531 31.210 157 101 5 6 157 8 162 6.79e-21 85.1
Msa0300320 MtrunA17_Chr1g0199331 33.136 169 103 7 7 168 32 197 6.79e-21 84.7
Msa0300320 MtrunA17_Chr1g0174281 34.615 156 95 6 7 157 16 169 1.34e-20 84.7
Msa0300320 MtrunA17_Chr4g0055651 29.936 157 103 5 6 157 8 162 2.15e-20 83.6
Msa0300320 MtrunA17_Chr5g0400131 34.194 155 95 5 8 157 11 163 5.38e-20 82.8
Msa0300320 MtrunA17_Chr2g0276931 30.573 157 102 5 6 157 9 163 7.58e-20 82.4
Msa0300320 MtrunA17_Chr8g0381841 34.194 155 95 5 8 157 19 171 8.51e-20 82.4
Msa0300320 MtrunA17_Chr3g0112681 33.962 159 94 5 6 157 12 166 1.05e-19 82.0
Msa0300320 MtrunA17_Chr3g0123971 33.548 155 96 5 8 157 11 163 1.63e-19 81.3
Msa0300320 MtrunA17_Chr2g0327911 31.250 160 101 5 5 157 14 171 2.08e-19 81.3
Msa0300320 MtrunA17_Chr8g0382221 32.903 155 97 5 8 157 11 163 2.65e-19 80.9
Msa0300320 MtrunA17_Chr4g0032201 31.847 157 100 5 6 157 12 166 3.53e-19 80.9
Msa0300320 MtrunA17_Chr8g0346421 37.719 114 68 3 8 119 28 140 5.32e-19 80.5
Msa0300320 MtrunA17_Chr3g0123821 32.903 155 97 5 8 157 18 170 5.34e-19 80.5
Msa0300320 MtrunA17_Chr7g0231131 32.051 156 99 5 7 157 16 169 5.68e-19 80.5
Msa0300320 MtrunA17_Chr2g0319951 31.847 157 100 5 6 157 13 167 5.75e-19 80.1
Msa0300320 MtrunA17_Chr8g0390561 30.723 166 108 5 8 168 11 174 8.38e-19 79.3
Msa0300320 MtrunA17_Chr7g0275991 32.258 155 98 5 8 157 22 174 1.02e-18 79.7
Msa0300320 MtrunA17_Chr1g0197331 30.723 166 106 6 7 164 16 180 1.85e-18 79.0
Msa0300320 MtrunA17_Chr7g0234201 31.707 164 105 5 6 164 13 174 2.02e-18 79.0
Msa0300320 MtrunA17_Chr8g0349681 28.395 162 109 5 1 157 8 167 2.11e-18 81.3
Msa0300320 MtrunA17_Chr8g0349681 28.395 162 109 5 1 157 379 538 5.23e-18 80.5
Msa0300320 MtrunA17_Chr8g0349681 27.160 162 111 5 1 157 170 329 1.69e-17 79.0
Msa0300320 MtrunA17_Chr2g0276941 31.902 163 100 5 6 161 15 173 2.75e-18 78.6
Msa0300320 MtrunA17_Chr7g0214241 30.488 164 107 5 6 164 15 176 4.95e-18 77.8
Msa0300320 MtrunA17_Chr2g0315551 31.210 157 101 5 6 157 13 167 7.93e-18 77.4
Msa0300320 MtrunA17_Chr1g0201121 30.337 178 110 6 1 165 1 177 8.08e-18 77.0
Msa0300320 MtrunA17_Chr8g0392931 32.692 156 100 4 6 157 14 168 8.59e-18 77.0
Msa0300320 MtrunA17_Chr8g0351861 31.210 157 101 5 6 157 16 170 1.89e-17 76.6
Msa0300320 MtrunA17_Chr8g0383261 29.586 169 107 4 6 163 12 179 2.71e-17 75.5
Msa0300320 MtrunA17_Chr4g0040781 36.207 116 71 3 6 119 13 127 3.88e-17 75.5
Msa0300320 MtrunA17_Chr4g0046261 35.345 116 72 3 6 119 13 127 4.54e-17 75.1
Msa0300320 MtrunA17_Chr2g0283131 35.345 116 72 3 6 119 13 127 4.94e-17 75.1
Msa0300320 MtrunA17_Chr1g0189361 31.098 164 106 5 6 164 14 175 5.10e-17 75.1
Msa0300320 MtrunA17_Chr3g0109721 30.769 156 99 5 8 157 12 164 5.44e-17 74.7
Msa0300320 MtrunA17_Chr6g0466301 29.448 163 103 5 6 157 8 169 6.81e-17 74.7
Msa0300320 MtrunA17_Chr6g0465491 29.448 163 103 5 6 157 8 169 6.81e-17 74.7
Msa0300320 MtrunA17_Chr2g0331691 29.651 172 109 5 6 166 10 180 7.81e-17 74.3
Msa0300320 MtrunA17_Chr5g0433561 29.870 154 103 4 8 157 12 164 3.74e-16 72.4
Msa0300320 MtrunA17_Chr4g0034871 28.324 173 112 4 6 167 8 179 6.05e-16 72.0
Msa0300320 MtrunA17_Chr4g0004661 29.240 171 109 5 7 166 65 234 1.08e-15 72.4
Msa0300320 MtrunA17_Chr2g0325091 34.109 129 82 3 6 132 11 138 3.89e-15 70.1
Msa0300320 MtrunA17_Chr6g0482721 30.128 156 104 4 6 157 12 166 5.07e-15 69.7
Msa0300320 MtrunA17_Chr4g0046221 78.378 37 8 0 129 165 10 46 3.72e-14 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300320 AT5G45970.1 82.682 179 18 1 1 166 1 179 1.47e-107 305
Msa0300320 AT4G35020.1 81.006 179 21 1 1 166 1 179 4.49e-107 304
Msa0300320 AT4G35020.2 81.006 179 21 1 1 166 1 179 4.49e-107 304
Msa0300320 AT4G35020.3 81.006 179 21 1 1 166 1 179 4.49e-107 304
Msa0300320 AT1G75840.1 82.022 178 19 1 1 165 1 178 1.21e-106 303
Msa0300320 AT1G20090.1 84.000 175 15 1 4 165 3 177 1.84e-106 302
Msa0300320 AT3G51300.1 80.899 178 21 1 1 165 1 178 2.10e-105 300
Msa0300320 AT2G17800.2 80.899 178 21 1 1 165 1 178 8.19e-105 298
Msa0300320 AT2G17800.1 80.899 178 21 1 1 165 1 178 8.19e-105 298
Msa0300320 AT4G35950.1 79.775 178 23 1 1 165 1 178 1.09e-103 295
Msa0300320 AT3G48040.1 75.000 180 32 1 2 168 4 183 4.63e-99 284
Msa0300320 AT5G62880.1 75.281 178 31 1 2 166 4 181 1.02e-98 284
Msa0300320 AT4G28950.1 75.706 177 32 1 1 166 1 177 2.22e-98 283
Msa0300320 AT2G44690.1 76.437 174 28 1 6 166 18 191 2.61e-96 277
Msa0300320 AT5G62880.2 73.256 172 29 2 2 160 4 171 7.85e-91 263
Msa0300320 AT3G48040.2 74.847 163 28 1 2 151 4 166 8.09e-90 260
Msa0300320 AT4G35020.4 90.083 121 12 0 1 121 1 121 1.65e-82 240
Msa0300320 AT4G35020.5 90.083 121 12 0 1 121 1 121 1.65e-82 240
Msa0300320 AT3G51290.2 79.310 145 17 1 34 165 635 779 6.35e-76 241
Msa0300320 AT4G35860.1 32.164 171 109 5 1 166 1 169 4.75e-25 96.3
Msa0300320 AT3G46060.2 34.177 158 96 5 6 157 15 170 8.76e-25 95.9
Msa0300320 AT3G46060.3 34.177 158 96 5 6 157 15 170 8.76e-25 95.9
Msa0300320 AT3G46060.1 34.177 158 96 5 6 157 15 170 8.76e-25 95.9
Msa0300320 AT1G43890.3 32.934 167 104 5 6 166 13 177 9.93e-25 95.5
Msa0300320 AT1G43890.1 32.934 167 104 5 6 166 13 177 9.93e-25 95.5
Msa0300320 AT1G43890.2 32.934 167 104 5 6 166 13 177 9.93e-25 95.5
Msa0300320 AT3G53610.2 32.335 167 105 5 6 166 15 179 3.77e-24 94.4
Msa0300320 AT3G53610.3 32.335 167 105 5 6 166 15 179 3.77e-24 94.4
Msa0300320 AT3G53610.1 32.335 167 105 5 6 166 15 179 3.77e-24 94.4
Msa0300320 AT4G17170.1 32.099 162 103 5 1 157 1 160 3.92e-24 94.0
Msa0300320 AT5G59840.1 32.335 167 105 5 6 166 15 179 7.45e-24 93.6
Msa0300320 AT3G54840.2 33.544 158 97 5 7 158 34 189 1.11e-22 89.7
Msa0300320 AT3G54840.1 33.544 158 97 5 7 158 34 189 1.80e-22 89.4
Msa0300320 AT1G18200.1 36.025 161 96 6 6 161 13 171 3.73e-22 89.4
Msa0300320 AT4G17160.1 30.864 162 105 5 1 157 1 160 3.76e-22 88.6
Msa0300320 AT3G54840.3 33.548 155 95 5 10 158 3 155 5.90e-22 87.4
Msa0300320 AT5G03530.2 31.707 164 103 6 8 164 15 176 1.51e-21 87.4
Msa0300320 AT5G03530.1 31.707 164 103 6 8 164 15 176 1.51e-21 87.4
Msa0300320 AT3G09900.1 34.132 167 102 5 6 166 15 179 2.44e-21 87.0
Msa0300320 AT4G39890.1 34.375 160 96 6 4 157 8 164 3.66e-21 86.3
Msa0300320 AT3G11730.1 30.573 157 102 5 6 157 8 162 4.80e-21 85.9
Msa0300320 AT4G19640.1 33.548 155 96 5 8 157 12 164 6.13e-21 85.5
Msa0300320 AT4G17530.1 31.548 168 105 6 6 165 8 173 9.17e-21 85.1
Msa0300320 AT1G02130.1 31.847 157 100 5 6 157 8 162 1.25e-20 84.7
Msa0300320 AT5G45130.1 33.548 155 96 5 8 157 12 164 1.26e-20 84.7
Msa0300320 AT3G12160.1 34.839 155 94 6 8 157 17 169 1.60e-20 84.7
Msa0300320 AT5G47200.1 31.548 168 105 6 6 165 8 173 1.63e-20 84.3
Msa0300320 AT5G03520.1 34.132 167 102 5 6 166 15 179 2.86e-20 84.0
Msa0300320 AT2G22290.1 35.484 155 93 5 8 157 11 163 3.70e-20 83.6
Msa0300320 AT2G43130.1 31.098 164 106 5 6 164 12 173 4.97e-20 83.2
Msa0300320 AT5G10260.1 34.194 155 95 5 8 157 11 163 5.41e-20 83.2
Msa0300320 AT1G73640.1 35.032 157 95 5 6 157 13 167 5.62e-20 83.6
Msa0300320 AT5G45130.2 33.333 153 95 5 10 157 22 172 6.00e-20 83.2
Msa0300320 AT5G64990.1 35.484 155 93 5 8 157 9 161 7.20e-20 82.8
Msa0300320 AT1G16920.1 37.931 116 69 3 6 119 13 127 7.63e-20 82.8
Msa0300320 AT2G31680.1 31.707 164 105 5 6 164 12 173 1.03e-19 82.8
Msa0300320 AT2G44610.1 33.548 155 96 5 8 157 11 163 1.43e-19 82.0
Msa0300320 AT1G09630.1 33.133 166 100 5 6 164 12 173 3.29e-19 81.3
Msa0300320 AT5G65270.1 33.548 155 96 5 8 157 19 171 3.49e-19 81.3
Msa0300320 AT5G45750.1 32.692 156 100 5 6 157 13 167 4.57e-19 80.9
Msa0300320 AT1G06400.1 33.333 156 99 5 6 157 13 167 5.88e-19 80.5
Msa0300320 AT1G22740.1 31.461 178 108 6 1 165 1 177 6.85e-19 80.1
Msa0300320 AT4G18800.1 32.051 156 101 5 6 157 13 167 8.81e-19 80.1
Msa0300320 AT5G59150.1 31.677 161 103 5 6 161 12 170 1.00e-18 80.1
Msa0300320 AT1G05810.1 31.677 161 103 5 6 161 12 170 1.09e-18 79.7
Msa0300320 AT1G05810.2 31.677 161 103 5 6 161 58 216 1.71e-18 80.1
Msa0300320 AT1G07410.1 31.847 157 100 5 6 157 12 166 2.42e-18 79.0
Msa0300320 AT3G46830.1 31.210 157 101 5 6 157 12 166 7.12e-18 77.8
Msa0300320 AT3G46830.2 31.210 157 101 5 6 157 12 166 7.12e-18 77.8
Msa0300320 AT2G33870.1 32.692 156 100 4 6 157 13 167 8.31e-18 77.4
Msa0300320 AT4G18430.1 32.692 156 100 4 6 157 13 167 9.16e-18 77.4
Msa0300320 AT5G47960.1 31.613 155 99 6 8 157 17 169 1.10e-17 77.4
Msa0300320 AT4G19640.2 35.088 114 71 3 8 119 12 124 1.61e-17 75.1
Msa0300320 AT4G39990.1 30.769 156 101 5 8 158 19 172 2.36e-17 76.6
Msa0300320 AT3G15060.1 34.109 129 82 3 8 134 15 142 2.58e-17 76.3
Msa0300320 AT5G47520.1 30.488 164 107 5 6 164 14 175 2.68e-17 76.3
Msa0300320 AT5G64990.2 33.951 162 93 6 8 157 9 168 3.47e-17 75.9
Msa0300320 AT3G18820.1 29.480 173 110 5 6 167 8 179 3.94e-17 75.5
Msa0300320 AT5G60860.1 30.769 156 103 4 6 157 13 167 4.54e-17 75.5
Msa0300320 AT1G28550.1 34.351 131 83 3 6 134 13 142 5.29e-17 75.5
Msa0300320 AT4G19640.3 35.345 116 68 4 8 119 12 124 5.34e-17 75.1
Msa0300320 AT3G07410.1 29.697 165 109 5 6 165 12 174 7.11e-17 75.1
Msa0300320 AT1G01200.1 34.848 132 79 4 8 133 30 160 7.95e-17 75.5
Msa0300320 AT2G21880.1 28.235 170 112 3 6 165 9 178 8.56e-17 74.7
Msa0300320 AT4G35860.2 32.258 124 78 4 47 166 2 123 1.98e-16 72.8
Msa0300320 AT3G16100.1 29.609 179 112 6 1 166 1 178 1.17e-15 71.6
Msa0300320 AT1G52280.1 29.143 175 110 6 1 162 1 174 2.05e-15 70.9
Msa0300320 AT4G09720.1 28.652 178 113 6 1 165 1 177 3.27e-15 70.5
Msa0300320 AT1G49300.2 28.834 163 104 5 6 157 8 169 1.41e-14 68.9
Msa0300320 AT1G49300.1 28.834 163 104 5 6 157 8 169 1.41e-14 68.9
Msa0300320 AT3G09910.3 28.659 164 108 6 8 164 15 176 1.54e-14 68.6
Msa0300320 AT3G09910.1 28.659 164 108 6 8 164 15 176 1.54e-14 68.6
Msa0300320 AT3G09910.2 28.659 164 108 6 8 164 15 176 1.54e-14 68.6
Msa0300320 AT3G09910.5 29.268 164 107 6 7 163 14 175 1.70e-14 68.2
Msa0300320 AT3G09910.4 29.268 164 107 6 7 163 14 175 1.70e-14 68.2
Msa0300320 AT5G03520.2 34.400 125 75 4 47 166 47 169 2.92e-14 67.8
Msa0300320 AT4G09720.4 27.322 183 114 5 1 165 1 182 6.58e-14 67.0
Msa0300320 AT5G39620.1 28.659 164 101 5 7 157 6 166 3.80e-13 64.7
Msa0300320 AT4G09720.3 26.455 189 114 5 1 165 1 188 5.06e-13 64.7
Msa0300320 AT5G59150.2 32.174 115 72 4 47 157 3 115 1.04e-11 60.5
Msa0300320 AT5G27540.1 22.093 172 127 2 3 168 14 184 1.06e-11 62.4
Msa0300320 AT5G27540.2 22.093 172 127 2 3 168 14 184 1.06e-11 62.4
Msa0300320 AT3G63150.1 28.402 169 112 5 7 168 15 181 4.81e-11 60.5
Msa0300320 AT2G21880.4 26.797 153 102 3 23 165 1 153 7.32e-11 58.5
Msa0300320 AT2G21880.3 26.797 153 102 3 23 165 1 153 7.32e-11 58.5

Find 55 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCTTTCTGTTTCTTTA+AGG 0.156260 2_3:+55665139 None:intergenic
GCACAGTTAATCTTGGATTA+TGG 0.198843 2_3:-55664598 Msa0300320:CDS
TCAATACAAGCAGGAGAATT+TGG 0.242087 2_3:-55662797 Msa0300320:three_prime_UTR
CAAACATGAATACCGATTAA+AGG 0.301861 2_3:+55663020 None:intergenic
ATTTCCTAGTTATATGTAAC+TGG 0.306750 2_3:-55662891 Msa0300320:three_prime_UTR
TTTAGAGATATTCTCATAAC+TGG 0.310909 2_3:+55664190 None:intergenic
TTATGAGAATATCTCTAAAA+AGG 0.315067 2_3:-55664186 Msa0300320:intron
ATGCTGTGACATTGATTTCA+TGG 0.329209 2_3:-55662990 Msa0300320:three_prime_UTR
TGTTTAGCTCCTACCAAGTT+TGG 0.332301 2_3:+55664020 None:intergenic
TTAGCTTTCTGTTTCTTTAA+GGG 0.348554 2_3:+55665140 None:intergenic
CTTTAATCGGTATTCATGTT+TGG 0.355137 2_3:-55663019 Msa0300320:three_prime_UTR
CACAGTTAATCTTGGATTAT+GGG 0.369623 2_3:-55664597 Msa0300320:CDS
GGGAAGTGAAGCATTTAATA+AGG 0.384022 2_3:+55665061 None:intergenic
AGTGCAAATGTTGTGGTTGA+TGG 0.385099 2_3:-55664623 Msa0300320:CDS
TTGGAATACATTTGTTCAAA+AGG 0.391256 2_3:+55662962 None:intergenic
CAGGACAAGAGGATTATAAC+AGG 0.405871 2_3:-55664277 Msa0300320:CDS
CTTAGGCCTTTGAGCTATAG+AGG 0.408891 2_3:-55664254 Msa0300320:CDS
GATGGCAGCACAGTTAATCT+TGG 0.412892 2_3:-55664605 Msa0300320:CDS
GGAGCATAATGTCTCAGTTC+AGG 0.421519 2_3:+55664069 None:intergenic
TTGGTTCCCACAAGTACAAT+TGG 0.428473 2_3:+55664039 None:intergenic
GAAGAGCTGAAGAGGGCAAT+TGG 0.442300 2_3:-55663325 Msa0300320:CDS
TTCATCAAATGTGTTACTGT+TGG 0.452151 2_3:-55664829 Msa0300320:CDS
TGTAGTGTTAAGAAAGAAAG+AGG 0.469853 2_3:+55665007 None:intergenic
GTACTTGTGGGAACCAAACT+TGG 0.487335 2_3:-55664033 Msa0300320:intron
AAGAGGATTATAACAGGCTT+AGG 0.490120 2_3:-55664271 Msa0300320:CDS
TGTGTTTGATGCTGCAATCA+AGG 0.491064 2_3:-55663101 Msa0300320:CDS
AACTGAAAGAAAGTAGAGCT+TGG 0.498823 2_3:+55662943 None:intergenic
CTTGTTTGGTGTTGTAGTAG+TGG 0.499810 2_3:+55665196 None:intergenic
ACAAGTACAATTGGCACAGT+TGG 0.513226 2_3:+55664048 None:intergenic
AGGAGCAGATGTGTTCTTGT+TGG 0.514278 2_3:-55664234 Msa0300320:CDS
ATTTAATAAGGCAAGTCCTA+TGG 0.517827 2_3:+55665073 None:intergenic
GGCTCTGCTGAGTAGTGAAA+AGG 0.521685 2_3:+55664211 None:intergenic
TTTCTGTTTCTTTAAGGGGA+AGG 0.524640 2_3:+55665145 None:intergenic
AACTGTGCCAATTGTACTTG+TGG 0.525586 2_3:-55664046 Msa0300320:CDS
TGCAATATTTCAGAATGTGA+AGG 0.527117 2_3:-55663125 Msa0300320:intron
TAGCTTTCTGTTTCTTTAAG+GGG 0.533652 2_3:+55665141 None:intergenic
CTTATCACAAAATTTATCCA+TGG 0.538708 2_3:+55662864 None:intergenic
GGCAGGGTGAAGAGCTGAAG+AGG 0.540183 2_3:-55663333 Msa0300320:intron
TACAAGCAGGAGAATTTGGT+TGG 0.540898 2_3:-55662793 Msa0300320:three_prime_UTR
CACACTAAAGTTGGTGCCAT+AGG 0.549969 2_3:-55665089 Msa0300320:five_prime_UTR
TATCAATCGTCAATACAAGC+AGG 0.559573 2_3:-55662806 Msa0300320:three_prime_UTR
TACAGCTATAACTACTGCCC+AGG 0.559919 2_3:-55663579 Msa0300320:intron
ATGCAGCTCAAAGACTCAGC+AGG 0.566907 2_3:-55663284 Msa0300320:intron
CTTGGATTATGGGACACTGC+TGG 0.569014 2_3:-55664587 Msa0300320:intron
GTAGTGTTAAGAAAGAAAGA+GGG 0.583641 2_3:+55665008 None:intergenic
ATATCCAGTTACATATAACT+AGG 0.585968 2_3:+55662887 None:intergenic
CAGTGATGAAAACAACAAAA+CGG 0.589997 2_3:-55664915 Msa0300320:five_prime_UTR
ACTGTGCCAATTGTACTTGT+GGG 0.592943 2_3:-55664045 Msa0300320:CDS
TATATGTAACTGGATATCCA+TGG 0.598619 2_3:-55662881 Msa0300320:three_prime_UTR
CACAAGCAATACATTCCCTA+CGG 0.607441 2_3:-55664767 Msa0300320:intron
ACTGTTGGAGATGGTGCTGT+TGG 0.608648 2_3:-55664814 Msa0300320:CDS
TAACTTCAGTGCAAATGTTG+TGG 0.625689 2_3:-55664630 Msa0300320:CDS
GCAGGGTGAAGAGCTGAAGA+GGG 0.625808 2_3:-55663332 Msa0300320:intron
TCTGCTCCTCTATAGCTCAA+AGG 0.639148 2_3:+55664248 None:intergenic
AAATGTGTTACTGTTGGAGA+TGG 0.644647 2_3:-55664823 Msa0300320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTCATAAAAATTC+AGG - chr2_3:55663690-55663709 Msa0300320:intron 10.0%
!! TAAACATATATAACTTAATT+GGG - chr2_3:55663983-55664002 Msa0300320:intron 10.0%
!!! TAATTTTTTCATAAAAATTC+AGG - chr2_3:55663690-55663709 Msa0300320:intron 10.0%
!! TAAACATATATAACTTAATT+GGG - chr2_3:55663983-55664002 Msa0300320:intron 10.0%
!!! ATTTGAATTTTGAATTGAAA+AGG + chr2_3:55662953-55662972 None:intergenic 15.0%
!!! TAACAAATTTAGTTTATGTT+TGG - chr2_3:55663274-55663293 Msa0300320:intron 15.0%
!! ACAAATTATAAATCGTAAAA+CGG + chr2_3:55663455-55663474 None:intergenic 15.0%
!! ATGAAAAAATTAGTAAATCT+TGG + chr2_3:55663682-55663701 None:intergenic 15.0%
!! CTAAACATATATAACTTAAT+TGG - chr2_3:55663982-55664001 Msa0300320:intron 15.0%
!! AAGTTAAGTAGTATTAATTA+GGG + chr2_3:55664163-55664182 None:intergenic 15.0%
!! ACACAAATAATGTAAAATTT+AGG - chr2_3:55664632-55664651 Msa0300320:CDS 15.0%
!! TTAACATAATCAGAAATTAT+TGG - chr2_3:55664766-55664785 Msa0300320:intron 15.0%
!!! ATTTGAATTTTGAATTGAAA+AGG + chr2_3:55662953-55662972 None:intergenic 15.0%
!!! TAACAAATTTAGTTTATGTT+TGG - chr2_3:55663274-55663293 Msa0300320:intron 15.0%
!! ACAAATTATAAATCGTAAAA+CGG + chr2_3:55663455-55663474 None:intergenic 15.0%
!! ATGAAAAAATTAGTAAATCT+TGG + chr2_3:55663682-55663701 None:intergenic 15.0%
!! CTAAACATATATAACTTAAT+TGG - chr2_3:55663982-55664001 Msa0300320:intron 15.0%
!! AAGTTAAGTAGTATTAATTA+GGG + chr2_3:55664163-55664182 None:intergenic 15.0%
!! ACACAAATAATGTAAAATTT+AGG - chr2_3:55664632-55664651 Msa0300320:CDS 15.0%
!! TTAACATAATCAGAAATTAT+TGG - chr2_3:55664766-55664785 Msa0300320:intron 15.0%
!!! TTATGAGAATATCTCTAAAA+AGG - chr2_3:55663800-55663819 Msa0300320:intron 20.0%
!!! TTCACTTGTGAATTTTTATA+TGG - chr2_3:55663843-55663862 Msa0300320:intron 20.0%
!! CAAAAATAAAATCAAATCTG+TGG + chr2_3:55664079-55664098 None:intergenic 20.0%
!! AGTTAAGTAGTATTAATTAG+GGG + chr2_3:55664162-55664181 None:intergenic 20.0%
!! CAAGTTAAGTAGTATTAATT+AGG + chr2_3:55664164-55664183 None:intergenic 20.0%
!! AATTAATACAATCACAACAA+AGG - chr2_3:55664204-55664223 Msa0300320:CDS 20.0%
!! ATTCCAAATAGTATTTAAGT+TGG - chr2_3:55664515-55664534 Msa0300320:intron 20.0%
!! AAATAATGTAAAATTTAGGC+AGG - chr2_3:55664636-55664655 Msa0300320:CDS 20.0%
!! AATAATGTAAAATTTAGGCA+GGG - chr2_3:55664637-55664656 Msa0300320:CDS 20.0%
!!! ATCATGTATTTTCCTTTAAT+CGG - chr2_3:55664954-55664973 Msa0300320:five_prime_UTR 20.0%
!!! TTTTCTTGTATTTTTGTTGA+TGG - chr2_3:55665064-55665083 Msa0300320:five_prime_UTR 20.0%
!!! TTATGAGAATATCTCTAAAA+AGG - chr2_3:55663800-55663819 Msa0300320:intron 20.0%
!!! TTCACTTGTGAATTTTTATA+TGG - chr2_3:55663843-55663862 Msa0300320:intron 20.0%
!! CAAAAATAAAATCAAATCTG+TGG + chr2_3:55664079-55664098 None:intergenic 20.0%
!! AGTTAAGTAGTATTAATTAG+GGG + chr2_3:55664162-55664181 None:intergenic 20.0%
!! CAAGTTAAGTAGTATTAATT+AGG + chr2_3:55664164-55664183 None:intergenic 20.0%
!! AATTAATACAATCACAACAA+AGG - chr2_3:55664204-55664223 Msa0300320:CDS 20.0%
!! ATTCCAAATAGTATTTAAGT+TGG - chr2_3:55664515-55664534 Msa0300320:intron 20.0%
!! AAATAATGTAAAATTTAGGC+AGG - chr2_3:55664636-55664655 Msa0300320:CDS 20.0%
!! AATAATGTAAAATTTAGGCA+GGG - chr2_3:55664637-55664656 Msa0300320:CDS 20.0%
!!! ATCATGTATTTTCCTTTAAT+CGG - chr2_3:55664954-55664973 Msa0300320:five_prime_UTR 20.0%
!!! TTTTCTTGTATTTTTGTTGA+TGG - chr2_3:55665064-55665083 Msa0300320:five_prime_UTR 20.0%
!!! AGTTTTTAGATCAGCAAAAA+AGG + chr2_3:55662817-55662836 None:intergenic 25.0%
!! TTAGCTTTCTGTTTCTTTAA+GGG + chr2_3:55662849-55662868 None:intergenic 25.0%
!!! TTTAGCTTTCTGTTTCTTTA+AGG + chr2_3:55662850-55662869 None:intergenic 25.0%
!! AATTTTGAATTGAAAAGGAG+GGG + chr2_3:55662948-55662967 None:intergenic 25.0%
!! GAATTTTGAATTGAAAAGGA+GGG + chr2_3:55662949-55662968 None:intergenic 25.0%
!! TGAATTTTGAATTGAAAAGG+AGG + chr2_3:55662950-55662969 None:intergenic 25.0%
!!! GTTCAAATATTTTCTAGTGA+AGG - chr2_3:55663030-55663049 Msa0300320:three_prime_UTR 25.0%
!!! TCTTGTTCTTGTTTTTTTGA+AGG - chr2_3:55663311-55663330 Msa0300320:CDS 25.0%
! TAAGTAGCAAAGACATTTAA+CGG - chr2_3:55663488-55663507 Msa0300320:intron 25.0%
! TTTAGAGATATTCTCATAAC+TGG + chr2_3:55663799-55663818 None:intergenic 25.0%
! TGCATCTAGTAATTTGAAAA+GGG + chr2_3:55664117-55664136 None:intergenic 25.0%
! ATGCATCTAGTAATTTGAAA+AGG + chr2_3:55664118-55664137 None:intergenic 25.0%
! TAGAAATGTGAAAAATGCAA+AGG - chr2_3:55664283-55664302 Msa0300320:intron 25.0%
! AGAAATGTGAAAAATGCAAA+GGG - chr2_3:55664284-55664303 Msa0300320:intron 25.0%
! TCATACAAATTTGTAGGAAT+TGG - chr2_3:55664434-55664453 Msa0300320:intron 25.0%
! ATGCCAACTTAAATACTATT+TGG + chr2_3:55664521-55664540 None:intergenic 25.0%
! GCATATATAGTTATAACTCA+TGG - chr2_3:55664739-55664758 Msa0300320:intron 25.0%
!!! CTTTCTTTTTTTCTTTGCTT+AGG + chr2_3:55664926-55664945 None:intergenic 25.0%
! TTGGAATACATTTGTTCAAA+AGG + chr2_3:55665027-55665046 None:intergenic 25.0%
! ATTTCCTAGTTATATGTAAC+TGG - chr2_3:55665095-55665114 Msa0300320:five_prime_UTR 25.0%
! ATATCCAGTTACATATAACT+AGG + chr2_3:55665102-55665121 None:intergenic 25.0%
! CTTATCACAAAATTTATCCA+TGG + chr2_3:55665125-55665144 None:intergenic 25.0%
!!! AGTTTTTAGATCAGCAAAAA+AGG + chr2_3:55662817-55662836 None:intergenic 25.0%
!! TTAGCTTTCTGTTTCTTTAA+GGG + chr2_3:55662849-55662868 None:intergenic 25.0%
!!! TTTAGCTTTCTGTTTCTTTA+AGG + chr2_3:55662850-55662869 None:intergenic 25.0%
!! AATTTTGAATTGAAAAGGAG+GGG + chr2_3:55662948-55662967 None:intergenic 25.0%
!! GAATTTTGAATTGAAAAGGA+GGG + chr2_3:55662949-55662968 None:intergenic 25.0%
!! TGAATTTTGAATTGAAAAGG+AGG + chr2_3:55662950-55662969 None:intergenic 25.0%
!!! GTTCAAATATTTTCTAGTGA+AGG - chr2_3:55663030-55663049 Msa0300320:three_prime_UTR 25.0%
!!! TCTTGTTCTTGTTTTTTTGA+AGG - chr2_3:55663311-55663330 Msa0300320:CDS 25.0%
! TAAGTAGCAAAGACATTTAA+CGG - chr2_3:55663488-55663507 Msa0300320:intron 25.0%
! TTTAGAGATATTCTCATAAC+TGG + chr2_3:55663799-55663818 None:intergenic 25.0%
! TGCATCTAGTAATTTGAAAA+GGG + chr2_3:55664117-55664136 None:intergenic 25.0%
! ATGCATCTAGTAATTTGAAA+AGG + chr2_3:55664118-55664137 None:intergenic 25.0%
! TAGAAATGTGAAAAATGCAA+AGG - chr2_3:55664283-55664302 Msa0300320:intron 25.0%
! AGAAATGTGAAAAATGCAAA+GGG - chr2_3:55664284-55664303 Msa0300320:intron 25.0%
! TCATACAAATTTGTAGGAAT+TGG - chr2_3:55664434-55664453 Msa0300320:intron 25.0%
! ATGCCAACTTAAATACTATT+TGG + chr2_3:55664521-55664540 None:intergenic 25.0%
! GCATATATAGTTATAACTCA+TGG - chr2_3:55664739-55664758 Msa0300320:intron 25.0%
!!! CTTTCTTTTTTTCTTTGCTT+AGG + chr2_3:55664926-55664945 None:intergenic 25.0%
! TTGGAATACATTTGTTCAAA+AGG + chr2_3:55665027-55665046 None:intergenic 25.0%
! ATTTCCTAGTTATATGTAAC+TGG - chr2_3:55665095-55665114 Msa0300320:five_prime_UTR 25.0%
! ATATCCAGTTACATATAACT+AGG + chr2_3:55665102-55665121 None:intergenic 25.0%
! CTTATCACAAAATTTATCCA+TGG + chr2_3:55665125-55665144 None:intergenic 25.0%
! TAGCTTTCTGTTTCTTTAAG+GGG + chr2_3:55662848-55662867 None:intergenic 30.0%
AAAAAGAGTCACACTAAAGT+TGG - chr2_3:55662888-55662907 Msa0300320:three_prime_UTR 30.0%
ATTTAATAAGGCAAGTCCTA+TGG + chr2_3:55662916-55662935 None:intergenic 30.0%
GTAGTGTTAAGAAAGAAAGA+GGG + chr2_3:55662981-55663000 None:intergenic 30.0%
TGTAGTGTTAAGAAAGAAAG+AGG + chr2_3:55662982-55663001 None:intergenic 30.0%
CAGTGATGAAAACAACAAAA+CGG - chr2_3:55663071-55663090 Msa0300320:exon 30.0%
!! CTTTGCTTTGAAAGAAAAAC+AGG - chr2_3:55663103-55663122 Msa0300320:CDS 30.0%
! TTCATCAAATGTGTTACTGT+TGG - chr2_3:55663157-55663176 Msa0300320:intron 30.0%
CTGAAGTTATCAAAAACTGT+AGG + chr2_3:55663345-55663364 None:intergenic 30.0%
CACAGTTAATCTTGGATTAT+GGG - chr2_3:55663389-55663408 Msa0300320:intron 30.0%
! TGCTGGTAAGAATTTTCTTT+AGG - chr2_3:55663416-55663435 Msa0300320:intron 30.0%
! TGGTAAGAATTTTCTTTAGG+TGG - chr2_3:55663419-55663438 Msa0300320:intron 30.0%
CACAAAAACAACATTCTATC+TGG + chr2_3:55663582-55663601 None:intergenic 30.0%
ACTATTAATTACACACAGAG+AGG + chr2_3:55663649-55663668 None:intergenic 30.0%
TCATAAAAATTCAGGACAAG+AGG - chr2_3:55663698-55663717 Msa0300320:intron 30.0%
TTACGATAAAAGTTTAGCCT+TGG - chr2_3:55664019-55664038 Msa0300320:intron 30.0%
! GGAGTATTAAATTACTACCA+AGG + chr2_3:55664039-55664058 None:intergenic 30.0%
TGAAAAGGGGGTTATATTTA+GGG + chr2_3:55664103-55664122 None:intergenic 30.0%
TTGAAAAGGGGGTTATATTT+AGG + chr2_3:55664104-55664123 None:intergenic 30.0%
CATCTAGTAATTTGAAAAGG+GGG + chr2_3:55664115-55664134 None:intergenic 30.0%
GCATCTAGTAATTTGAAAAG+GGG + chr2_3:55664116-55664135 None:intergenic 30.0%
AATCACAACAAAGGACAATT+TGG - chr2_3:55664213-55664232 Msa0300320:CDS 30.0%
GAAATGTGAAAAATGCAAAG+GGG - chr2_3:55664285-55664304 Msa0300320:intron 30.0%
AAATGTGAAAAATGCAAAGG+GGG - chr2_3:55664286-55664305 Msa0300320:intron 30.0%
!! TATTATTGACTTGCAGATTG+AGG - chr2_3:55664351-55664370 Msa0300320:intron 30.0%
!! ATTATTGACTTGCAGATTGA+GGG - chr2_3:55664352-55664371 Msa0300320:intron 30.0%
CAAATTTGTATGACTTACCT+GGG + chr2_3:55664427-55664446 None:intergenic 30.0%
ACAAATTTGTATGACTTACC+TGG + chr2_3:55664428-55664447 None:intergenic 30.0%
GGTAAGTCATACAAATTTGT+AGG - chr2_3:55664428-55664447 Msa0300320:intron 30.0%
TTAGTACAAAGCTAATAGTG+TGG + chr2_3:55664827-55664846 None:intergenic 30.0%
TGCAATATTTCAGAATGTGA+AGG - chr2_3:55664861-55664880 Msa0300320:exon 30.0%
!!! TCTTTTTTTCTTTGCTTAGG+AGG + chr2_3:55664923-55664942 None:intergenic 30.0%
! CAAACATGAATACCGATTAA+AGG + chr2_3:55664969-55664988 None:intergenic 30.0%
CTTTAATCGGTATTCATGTT+TGG - chr2_3:55664967-55664986 Msa0300320:five_prime_UTR 30.0%
TATATGTAACTGGATATCCA+TGG - chr2_3:55665105-55665124 Msa0300320:five_prime_UTR 30.0%
! TAGCTTTCTGTTTCTTTAAG+GGG + chr2_3:55662848-55662867 None:intergenic 30.0%
AAAAAGAGTCACACTAAAGT+TGG - chr2_3:55662888-55662907 Msa0300320:three_prime_UTR 30.0%
ATTTAATAAGGCAAGTCCTA+TGG + chr2_3:55662916-55662935 None:intergenic 30.0%
GTAGTGTTAAGAAAGAAAGA+GGG + chr2_3:55662981-55663000 None:intergenic 30.0%
TGTAGTGTTAAGAAAGAAAG+AGG + chr2_3:55662982-55663001 None:intergenic 30.0%
CAGTGATGAAAACAACAAAA+CGG - chr2_3:55663071-55663090 Msa0300320:exon 30.0%
!! CTTTGCTTTGAAAGAAAAAC+AGG - chr2_3:55663103-55663122 Msa0300320:CDS 30.0%
! TTCATCAAATGTGTTACTGT+TGG - chr2_3:55663157-55663176 Msa0300320:intron 30.0%
CTGAAGTTATCAAAAACTGT+AGG + chr2_3:55663345-55663364 None:intergenic 30.0%
CACAGTTAATCTTGGATTAT+GGG - chr2_3:55663389-55663408 Msa0300320:intron 30.0%
! TGCTGGTAAGAATTTTCTTT+AGG - chr2_3:55663416-55663435 Msa0300320:intron 30.0%
! TGGTAAGAATTTTCTTTAGG+TGG - chr2_3:55663419-55663438 Msa0300320:intron 30.0%
CACAAAAACAACATTCTATC+TGG + chr2_3:55663582-55663601 None:intergenic 30.0%
ACTATTAATTACACACAGAG+AGG + chr2_3:55663649-55663668 None:intergenic 30.0%
TCATAAAAATTCAGGACAAG+AGG - chr2_3:55663698-55663717 Msa0300320:intron 30.0%
TTACGATAAAAGTTTAGCCT+TGG - chr2_3:55664019-55664038 Msa0300320:intron 30.0%
! GGAGTATTAAATTACTACCA+AGG + chr2_3:55664039-55664058 None:intergenic 30.0%
TGAAAAGGGGGTTATATTTA+GGG + chr2_3:55664103-55664122 None:intergenic 30.0%
TTGAAAAGGGGGTTATATTT+AGG + chr2_3:55664104-55664123 None:intergenic 30.0%
CATCTAGTAATTTGAAAAGG+GGG + chr2_3:55664115-55664134 None:intergenic 30.0%
GCATCTAGTAATTTGAAAAG+GGG + chr2_3:55664116-55664135 None:intergenic 30.0%
AATCACAACAAAGGACAATT+TGG - chr2_3:55664213-55664232 Msa0300320:CDS 30.0%
GAAATGTGAAAAATGCAAAG+GGG - chr2_3:55664285-55664304 Msa0300320:intron 30.0%
AAATGTGAAAAATGCAAAGG+GGG - chr2_3:55664286-55664305 Msa0300320:intron 30.0%
!! TATTATTGACTTGCAGATTG+AGG - chr2_3:55664351-55664370 Msa0300320:intron 30.0%
!! ATTATTGACTTGCAGATTGA+GGG - chr2_3:55664352-55664371 Msa0300320:intron 30.0%
CAAATTTGTATGACTTACCT+GGG + chr2_3:55664427-55664446 None:intergenic 30.0%
ACAAATTTGTATGACTTACC+TGG + chr2_3:55664428-55664447 None:intergenic 30.0%
GGTAAGTCATACAAATTTGT+AGG - chr2_3:55664428-55664447 Msa0300320:intron 30.0%
TTAGTACAAAGCTAATAGTG+TGG + chr2_3:55664827-55664846 None:intergenic 30.0%
TGCAATATTTCAGAATGTGA+AGG - chr2_3:55664861-55664880 Msa0300320:exon 30.0%
!!! TCTTTTTTTCTTTGCTTAGG+AGG + chr2_3:55664923-55664942 None:intergenic 30.0%
! CAAACATGAATACCGATTAA+AGG + chr2_3:55664969-55664988 None:intergenic 30.0%
CTTTAATCGGTATTCATGTT+TGG - chr2_3:55664967-55664986 Msa0300320:five_prime_UTR 30.0%
TATATGTAACTGGATATCCA+TGG - chr2_3:55665105-55665124 Msa0300320:five_prime_UTR 30.0%
! TCAGCAAAAAAGGACTTGTT+TGG + chr2_3:55662807-55662826 None:intergenic 35.0%
! TTTCTGTTTCTTTAAGGGGA+AGG + chr2_3:55662844-55662863 None:intergenic 35.0%
! GGGAAGTGAAGCATTTAATA+AGG + chr2_3:55662928-55662947 None:intergenic 35.0%
!! TGCTTTGAAAGAAAAACAGG+AGG - chr2_3:55663106-55663125 Msa0300320:CDS 35.0%
! AAATGTGTTACTGTTGGAGA+TGG - chr2_3:55663163-55663182 Msa0300320:intron 35.0%
! TGTGTTGTTATCATACCGTA+GGG + chr2_3:55663237-55663256 None:intergenic 35.0%
! ATGTGTTGTTATCATACCGT+AGG + chr2_3:55663238-55663257 None:intergenic 35.0%
TAACTTCAGTGCAAATGTTG+TGG - chr2_3:55663356-55663375 Msa0300320:intron 35.0%
GCACAGTTAATCTTGGATTA+TGG - chr2_3:55663388-55663407 Msa0300320:intron 35.0%
AAGAGGATTATAACAGGCTT+AGG - chr2_3:55663715-55663734 Msa0300320:intron 35.0%
! CACTAGCTAATTTTATGCAG+TGG - chr2_3:55663891-55663910 Msa0300320:intron 35.0%
TCAAATCTGTGGTCACATTA+AGG + chr2_3:55664068-55664087 None:intergenic 35.0%
CAGTATTTGATTGATCATCC+AGG - chr2_3:55664381-55664400 Msa0300320:intron 35.0%
! CACGCTATCAAGGTTTTTAT+AGG - chr2_3:55664599-55664618 Msa0300320:CDS 35.0%
!! ATGCTGTGACATTGATTTCA+TGG - chr2_3:55664996-55665015 Msa0300320:five_prime_UTR 35.0%
AACTGAAAGAAAGTAGAGCT+TGG + chr2_3:55665046-55665065 None:intergenic 35.0%
TATCAATCGTCAATACAAGC+AGG - chr2_3:55665180-55665199 Msa0300320:five_prime_UTR 35.0%
TCAATACAAGCAGGAGAATT+TGG - chr2_3:55665189-55665208 Msa0300320:five_prime_UTR 35.0%
! TCAGCAAAAAAGGACTTGTT+TGG + chr2_3:55662807-55662826 None:intergenic 35.0%
! TTTCTGTTTCTTTAAGGGGA+AGG + chr2_3:55662844-55662863 None:intergenic 35.0%
! GGGAAGTGAAGCATTTAATA+AGG + chr2_3:55662928-55662947 None:intergenic 35.0%
!! TGCTTTGAAAGAAAAACAGG+AGG - chr2_3:55663106-55663125 Msa0300320:CDS 35.0%
! AAATGTGTTACTGTTGGAGA+TGG - chr2_3:55663163-55663182 Msa0300320:intron 35.0%
! TGTGTTGTTATCATACCGTA+GGG + chr2_3:55663237-55663256 None:intergenic 35.0%
! ATGTGTTGTTATCATACCGT+AGG + chr2_3:55663238-55663257 None:intergenic 35.0%
TAACTTCAGTGCAAATGTTG+TGG - chr2_3:55663356-55663375 Msa0300320:intron 35.0%
GCACAGTTAATCTTGGATTA+TGG - chr2_3:55663388-55663407 Msa0300320:intron 35.0%
AAGAGGATTATAACAGGCTT+AGG - chr2_3:55663715-55663734 Msa0300320:intron 35.0%
! CACTAGCTAATTTTATGCAG+TGG - chr2_3:55663891-55663910 Msa0300320:intron 35.0%
TCAAATCTGTGGTCACATTA+AGG + chr2_3:55664068-55664087 None:intergenic 35.0%
CAGTATTTGATTGATCATCC+AGG - chr2_3:55664381-55664400 Msa0300320:intron 35.0%
! CACGCTATCAAGGTTTTTAT+AGG - chr2_3:55664599-55664618 Msa0300320:CDS 35.0%
!! ATGCTGTGACATTGATTTCA+TGG - chr2_3:55664996-55665015 Msa0300320:five_prime_UTR 35.0%
AACTGAAAGAAAGTAGAGCT+TGG + chr2_3:55665046-55665065 None:intergenic 35.0%
TATCAATCGTCAATACAAGC+AGG - chr2_3:55665180-55665199 Msa0300320:five_prime_UTR 35.0%
TCAATACAAGCAGGAGAATT+TGG - chr2_3:55665189-55665208 Msa0300320:five_prime_UTR 35.0%
CACAAGCAATACATTCCCTA+CGG - chr2_3:55663219-55663238 Msa0300320:intron 40.0%
AGTGCAAATGTTGTGGTTGA+TGG - chr2_3:55663363-55663382 Msa0300320:intron 40.0%
CAGGACAAGAGGATTATAAC+AGG - chr2_3:55663709-55663728 Msa0300320:intron 40.0%
ACAAGTACAATTGGCACAGT+TGG + chr2_3:55663941-55663960 None:intergenic 40.0%
AACTGTGCCAATTGTACTTG+TGG - chr2_3:55663940-55663959 Msa0300320:intron 40.0%
ACTGTGCCAATTGTACTTGT+GGG - chr2_3:55663941-55663960 Msa0300320:intron 40.0%
TTGGTTCCCACAAGTACAAT+TGG + chr2_3:55663950-55663969 None:intergenic 40.0%
! TGTTTAGCTCCTACCAAGTT+TGG + chr2_3:55663969-55663988 None:intergenic 40.0%
ATCTGTGGTCACATTAAGGA+CGG + chr2_3:55664064-55664083 None:intergenic 40.0%
TGCAAAGGGGGTTTACATTA+AGG - chr2_3:55664298-55664317 Msa0300320:intron 40.0%
GCAAAGGGGGTTTACATTAA+GGG - chr2_3:55664299-55664318 Msa0300320:intron 40.0%
AATTGAGAGATCTCAGTCGT+TGG + chr2_3:55664564-55664583 None:intergenic 40.0%
ATTGCGTTAACACGCTATCA+AGG - chr2_3:55664589-55664608 Msa0300320:CDS 40.0%
TGTGTTTGATGCTGCAATCA+AGG - chr2_3:55664885-55664904 Msa0300320:five_prime_UTR 40.0%
TACAAGCAGGAGAATTTGGT+TGG - chr2_3:55665193-55665212 Msa0300320:five_prime_UTR 40.0%
CACAAGCAATACATTCCCTA+CGG - chr2_3:55663219-55663238 Msa0300320:intron 40.0%
AGTGCAAATGTTGTGGTTGA+TGG - chr2_3:55663363-55663382 Msa0300320:intron 40.0%
CAGGACAAGAGGATTATAAC+AGG - chr2_3:55663709-55663728 Msa0300320:intron 40.0%
ACAAGTACAATTGGCACAGT+TGG + chr2_3:55663941-55663960 None:intergenic 40.0%
AACTGTGCCAATTGTACTTG+TGG - chr2_3:55663940-55663959 Msa0300320:intron 40.0%
ACTGTGCCAATTGTACTTGT+GGG - chr2_3:55663941-55663960 Msa0300320:intron 40.0%
TTGGTTCCCACAAGTACAAT+TGG + chr2_3:55663950-55663969 None:intergenic 40.0%
! TGTTTAGCTCCTACCAAGTT+TGG + chr2_3:55663969-55663988 None:intergenic 40.0%
ATCTGTGGTCACATTAAGGA+CGG + chr2_3:55664064-55664083 None:intergenic 40.0%
TGCAAAGGGGGTTTACATTA+AGG - chr2_3:55664298-55664317 Msa0300320:intron 40.0%
GCAAAGGGGGTTTACATTAA+GGG - chr2_3:55664299-55664318 Msa0300320:intron 40.0%
AATTGAGAGATCTCAGTCGT+TGG + chr2_3:55664564-55664583 None:intergenic 40.0%
ATTGCGTTAACACGCTATCA+AGG - chr2_3:55664589-55664608 Msa0300320:CDS 40.0%
TGTGTTTGATGCTGCAATCA+AGG - chr2_3:55664885-55664904 Msa0300320:five_prime_UTR 40.0%
!! CACACTAAAGTTGGTGCCAT+AGG - chr2_3:55662897-55662916 Msa0300320:three_prime_UTR 45.0%
GATGGCAGCACAGTTAATCT+TGG - chr2_3:55663381-55663400 Msa0300320:intron 45.0%
CTTAGGCCTTTGAGCTATAG+AGG - chr2_3:55663732-55663751 Msa0300320:intron 45.0%
TCTGCTCCTCTATAGCTCAA+AGG + chr2_3:55663741-55663760 None:intergenic 45.0%
AGGAGCAGATGTGTTCTTGT+TGG - chr2_3:55663752-55663771 Msa0300320:intron 45.0%
GGAGCATAATGTCTCAGTTC+AGG + chr2_3:55663920-55663939 None:intergenic 45.0%
GTACTTGTGGGAACCAAACT+TGG - chr2_3:55663953-55663972 Msa0300320:intron 45.0%
TTGTGGGAACCAAACTTGGT+AGG - chr2_3:55663957-55663976 Msa0300320:intron 45.0%
AGGGGGTTTACATTAAGGGA+CGG - chr2_3:55664303-55664322 Msa0300320:intron 45.0%
GGTTTACATTAAGGGACGGA+GGG - chr2_3:55664307-55664326 Msa0300320:intron 45.0%
GGACGGAGGGAGTAATTAAT+TGG - chr2_3:55664320-55664339 Msa0300320:intron 45.0%
GTAGTTATAGCTGTAGCTCC+TGG + chr2_3:55664402-55664421 None:intergenic 45.0%
TACAGCTATAACTACTGCCC+AGG - chr2_3:55664407-55664426 Msa0300320:intron 45.0%
CAGATTTCAGCCGCTAATGA+TGG - chr2_3:55664479-55664498 Msa0300320:intron 45.0%
AGTCGTCTAGCCATCATTAG+CGG + chr2_3:55664492-55664511 None:intergenic 45.0%
!! CACACTAAAGTTGGTGCCAT+AGG - chr2_3:55662897-55662916 Msa0300320:three_prime_UTR 45.0%
GATGGCAGCACAGTTAATCT+TGG - chr2_3:55663381-55663400 Msa0300320:intron 45.0%
CTTAGGCCTTTGAGCTATAG+AGG - chr2_3:55663732-55663751 Msa0300320:intron 45.0%
TCTGCTCCTCTATAGCTCAA+AGG + chr2_3:55663741-55663760 None:intergenic 45.0%
AGGAGCAGATGTGTTCTTGT+TGG - chr2_3:55663752-55663771 Msa0300320:intron 45.0%
GGAGCATAATGTCTCAGTTC+AGG + chr2_3:55663920-55663939 None:intergenic 45.0%
GTACTTGTGGGAACCAAACT+TGG - chr2_3:55663953-55663972 Msa0300320:intron 45.0%
TTGTGGGAACCAAACTTGGT+AGG - chr2_3:55663957-55663976 Msa0300320:intron 45.0%
AGGGGGTTTACATTAAGGGA+CGG - chr2_3:55664303-55664322 Msa0300320:intron 45.0%
GGTTTACATTAAGGGACGGA+GGG - chr2_3:55664307-55664326 Msa0300320:intron 45.0%
GGACGGAGGGAGTAATTAAT+TGG - chr2_3:55664320-55664339 Msa0300320:intron 45.0%
GTAGTTATAGCTGTAGCTCC+TGG + chr2_3:55664402-55664421 None:intergenic 45.0%
TACAGCTATAACTACTGCCC+AGG - chr2_3:55664407-55664426 Msa0300320:intron 45.0%
CAGATTTCAGCCGCTAATGA+TGG - chr2_3:55664479-55664498 Msa0300320:intron 45.0%
AGTCGTCTAGCCATCATTAG+CGG + chr2_3:55664492-55664511 None:intergenic 45.0%
!! ACTGTTGGAGATGGTGCTGT+TGG - chr2_3:55663172-55663191 Msa0300320:intron 50.0%
CTTGGATTATGGGACACTGC+TGG - chr2_3:55663399-55663418 Msa0300320:intron 50.0%
GGCTCTGCTGAGTAGTGAAA+AGG + chr2_3:55663778-55663797 None:intergenic 50.0%
GTGGTCACATTAAGGACGGA+GGG + chr2_3:55664060-55664079 None:intergenic 50.0%
TGTGGTCACATTAAGGACGG+AGG + chr2_3:55664061-55664080 None:intergenic 50.0%
GGGTTTACATTAAGGGACGG+AGG - chr2_3:55664306-55664325 Msa0300320:intron 50.0%
GAAGAGCTGAAGAGGGCAAT+TGG - chr2_3:55664661-55664680 Msa0300320:intron 50.0%
ATGCAGCTCAAAGACTCAGC+AGG - chr2_3:55664702-55664721 Msa0300320:intron 50.0%
!! ACTGTTGGAGATGGTGCTGT+TGG - chr2_3:55663172-55663191 Msa0300320:intron 50.0%
CTTGGATTATGGGACACTGC+TGG - chr2_3:55663399-55663418 Msa0300320:intron 50.0%
GGCTCTGCTGAGTAGTGAAA+AGG + chr2_3:55663778-55663797 None:intergenic 50.0%
GTGGTCACATTAAGGACGGA+GGG + chr2_3:55664060-55664079 None:intergenic 50.0%
TGTGGTCACATTAAGGACGG+AGG + chr2_3:55664061-55664080 None:intergenic 50.0%
GGGTTTACATTAAGGGACGG+AGG - chr2_3:55664306-55664325 Msa0300320:intron 50.0%
GAAGAGCTGAAGAGGGCAAT+TGG - chr2_3:55664661-55664680 Msa0300320:intron 50.0%
ATGCAGCTCAAAGACTCAGC+AGG - chr2_3:55664702-55664721 Msa0300320:intron 50.0%
GCAGGGTGAAGAGCTGAAGA+GGG - chr2_3:55664654-55664673 Msa0300320:CDS 55.0%
GCAGGGTGAAGAGCTGAAGA+GGG - chr2_3:55664654-55664673 Msa0300320:CDS 55.0%
GGCAGGGTGAAGAGCTGAAG+AGG - chr2_3:55664653-55664672 Msa0300320:CDS 60.0%
GGCAGGGTGAAGAGCTGAAG+AGG - chr2_3:55664653-55664672 Msa0300320:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55662793 55665215 55662793 ID=Msa0300320;Name=Msa0300320
chr2_3 mRNA 55662793 55665215 55662793 ID=Msa0300320-mRNA-1;Parent=Msa0300320;Name=Msa0300320-mRNA-1;_AED=0.11;_eAED=0.11;_QI=349|0.83|0.85|1|1|1|7|288|168
chr2_3 exon 55662793 55663134 55662793 ID=Msa0300320-mRNA-1:exon:17801;Parent=Msa0300320-mRNA-1
chr2_3 exon 55663285 55663350 55663285 ID=Msa0300320-mRNA-1:exon:17800;Parent=Msa0300320-mRNA-1
chr2_3 exon 55663580 55663605 55663580 ID=Msa0300320-mRNA-1:exon:17799;Parent=Msa0300320-mRNA-1
chr2_3 exon 55664034 55664097 55664034 ID=Msa0300320-mRNA-1:exon:17798;Parent=Msa0300320-mRNA-1
chr2_3 exon 55664187 55664296 55664187 ID=Msa0300320-mRNA-1:exon:17797;Parent=Msa0300320-mRNA-1
chr2_3 exon 55664588 55664675 55664588 ID=Msa0300320-mRNA-1:exon:17796;Parent=Msa0300320-mRNA-1
chr2_3 exon 55664768 55665215 55664768 ID=Msa0300320-mRNA-1:exon:17795;Parent=Msa0300320-mRNA-1
chr2_3 five_prime_UTR 55664867 55665215 55664867 ID=Msa0300320-mRNA-1:five_prime_utr;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55664768 55664866 55664768 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55664588 55664675 55664588 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55664187 55664296 55664187 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55664034 55664097 55664034 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55663580 55663605 55663580 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55663285 55663350 55663285 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 CDS 55663081 55663134 55663081 ID=Msa0300320-mRNA-1:cds;Parent=Msa0300320-mRNA-1
chr2_3 three_prime_UTR 55662793 55663080 55662793 ID=Msa0300320-mRNA-1:three_prime_utr;Parent=Msa0300320-mRNA-1
Gene Sequence

>Msa0300320

ATGAGTACAGCAAGATTCATCAAATGTGTTACTGTTGGAGATGGTGCTGTTGGAAAGACTTGTATGCTTATCTCTTACACAAGCAATACATTCCCTACGGATTATGTGCCTACAGTTTTTGATAACTTCAGTGCAAATGTTGTGGTTGATGGCAGCACAGTTAATCTTGGATTATGGGACACTGCTGGACAAGAGGATTATAACAGGCTTAGGCCTTTGAGCTATAGAGGAGCAGATGTGTTCTTGTTGGCCTTTTCACTACTCAGCAGAGCCAGTTATGAGAATATCTCTAAAAAGTGGATTCCTGAACTGAGACATTATGCTCCAACTGTGCCAATTGTACTTGTGGGAACCAAACTTGGAGCTACAGCTATAACTACTGCCCAGGGTGAAGAGCTGAAGAGGGCAATTGGTGCAGCAGTGTACTTAGAATGCAGCTCAAAGACTCAGCAGAATGTGAAGGCTGTGTTTGATGCTGCAATCAAGGTTGTTTTGCAAGCCTCCTAA
Protein sequence

>Msa0300320

MSTARFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLLSRASYENISKKWIPELRHYAPTVPIVLVGTKLGATAITTAQGEELKRAIGAAVYLECSSKTQQNVKAVFDAAIKVVLQAS*