AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATAATAAAGACAGTAAT+TGG 0.143839 7_4:-54526588 None:intergenic
ACCTCTGTCCTTTCGATTGT+TGG 0.220278 7_4:-54527079 None:intergenic
TAAATGTAAAATGACATAAA+AGG 0.275490 7_4:+54527811 Msa1163330:three_prime_UTR
CGGAAGGTTCGCCGTAGATT+TGG 0.296699 7_4:-54526961 None:intergenic
TTTCATTGCGAGCAAGAAAA+TGG 0.302176 7_4:+54527046 Msa1163330:CDS
GATTTCTGAATTGATTTGTC+TGG 0.304587 7_4:-54526877 None:intergenic
GTCCTTTCGATTGTTGGATT+TGG 0.306768 7_4:-54527073 None:intergenic
CTGATGTCGCTAAATAGATT+TGG 0.340818 7_4:-54526935 None:intergenic
GTGAACGGTTTGGATGATTT+AGG 0.346177 7_4:+54527619 Msa1163330:CDS
TGGGTTACAGTCTCGGGATT+TGG 0.348976 7_4:-54526656 None:intergenic
ACTCAAGGATCTTCATTTGC+TGG 0.354367 7_4:-54526676 None:intergenic
TTGAATGATTTATTAAAAGA+TGG 0.354525 7_4:-54526735 None:intergenic
GAGGGTGAAAATTGTAATAA+AGG 0.355968 7_4:-54526540 None:intergenic
GAAGTGTTTGGTAAAATTAA+CGG 0.394897 7_4:+54527775 Msa1163330:three_prime_UTR
CATTTGCTGGGTTACAGTCT+CGG 0.404264 7_4:-54526663 None:intergenic
ATTTGCTGGGTTACAGTCTC+GGG 0.417091 7_4:-54526662 None:intergenic
AAATAGACTTCGGCTCTGTT+TGG 0.419685 7_4:+54527644 Msa1163330:CDS
GATGAGTGAAAAGTAGAAGA+TGG 0.422281 7_4:-54526779 None:intergenic
AAATGTAAAATGACATAAAA+GGG 0.424634 7_4:+54527812 Msa1163330:three_prime_UTR
TGAATGATTTATTAAAAGAT+GGG 0.432525 7_4:-54526734 None:intergenic
CTCAAGGATCTTCATTTGCT+GGG 0.438191 7_4:-54526675 None:intergenic
GACAACAGCGGTGAACGGTT+TGG 0.449795 7_4:+54527609 Msa1163330:CDS
GATAAGCTAGTTGAAGTGTT+TGG 0.457354 7_4:+54527763 Msa1163330:three_prime_UTR
GGAAGAAATTTCGCTGGGTA+CGG 0.471476 7_4:+54527489 Msa1163330:CDS
GTCTGGATCTTTGTTAGAGA+AGG 0.478836 7_4:-54526860 None:intergenic
TCAAAGATCTTTGTTTGTTG+AGG 0.481153 7_4:-54526801 None:intergenic
ATGAGTGAAAAGTAGAAGAT+GGG 0.489032 7_4:-54526778 None:intergenic
TTGAAAGTGGTAGACGACTA+TGG 0.498220 7_4:+54527006 Msa1163330:exon
GAAACTGTTGATGGTGTGCC+TGG 0.501619 7_4:+54527568 Msa1163330:CDS
CTCAGATTTGCGACGACGGA+AGG 0.504998 7_4:-54526977 None:intergenic
AAATAATAAAGACAGTAATT+GGG 0.506270 7_4:-54526587 None:intergenic
GGATTTGGAAGAAATTTCGC+TGG 0.522746 7_4:+54527483 Msa1163330:CDS
AAATAGATTTGGTTCGCCGT+AGG 0.524650 7_4:-54526924 None:intergenic
ACTGTTGAAGAAACTGTTGA+TGG 0.557560 7_4:+54527559 Msa1163330:CDS
CAGTACCGTCGTCGAACCTA+CGG 0.572264 7_4:+54526908 Msa1163330:five_prime_UTR
ATCTGAGGAGCTGTTGAAAG+TGG 0.572889 7_4:+54526993 Msa1163330:five_prime_UTR
GCTCCTCAGATTTGCGACGA+CGG 0.578671 7_4:-54526981 None:intergenic
GATTTAGGTGAAATAGACTT+CGG 0.586821 7_4:+54527634 Msa1163330:CDS
GACATCAGAAACCAAATCTA+CGG 0.594769 7_4:+54526950 Msa1163330:five_prime_UTR
GATTTGACAACAGCGGTGAA+CGG 0.605276 7_4:+54527604 Msa1163330:CDS
GGTTCGACGACGGTACTGCA+AGG 0.610576 7_4:-54526903 None:intergenic
CTTCCGTCGTCGCAAATCTG+AGG 0.614071 7_4:+54526978 Msa1163330:five_prime_UTR
TACCAAATCCAACAATCGAA+AGG 0.626048 7_4:+54527071 Msa1163330:CDS
TACATTTGATTTGACAACAG+CGG 0.633090 7_4:+54527597 Msa1163330:CDS
TACGGAAAATTCATCTGACA+CGG 0.642825 7_4:+54527507 Msa1163330:CDS
GATTTGGAAGAAATTTCGCT+GGG 0.650948 7_4:+54527484 Msa1163330:CDS
ACTGTTGATGGTGTGCCTGG+TGG 0.662542 7_4:+54527571 Msa1163330:CDS
AAATCAAATGTACATCCACC+AGG 0.677854 7_4:-54527586 None:intergenic
TCCAACAATCGAAAGGACAG+AGG 0.680991 7_4:+54527078 Msa1163330:CDS
GTTCGCCGTAGGTTCGACGA+CGG 0.703266 7_4:-54526913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTCAAATTTTTATGT+TGG - chr7_4:54527349-54527368 None:intergenic 10.0%
!!! ATTATTTCAAATTTTTATGT+TGG - chr7_4:54527349-54527368 None:intergenic 10.0%
!!! AATCATATTTAATTTTGTGA+GGG - chr7_4:54526561-54526580 None:intergenic 15.0%
!! AAATAATAAAGACAGTAATT+GGG - chr7_4:54526590-54526609 None:intergenic 15.0%
!! AAAATAATAAAGACAGTAAT+TGG - chr7_4:54526591-54526610 None:intergenic 15.0%
!! TGAATGATTTATTAAAAGAT+GGG - chr7_4:54526737-54526756 None:intergenic 15.0%
!! TTGAATGATTTATTAAAAGA+TGG - chr7_4:54526738-54526757 None:intergenic 15.0%
!!! GGAAATTAGTTTTTTAATTA+AGG + chr7_4:54527139-54527158 Msa1163330:intron 15.0%
!!! TGAATCTTTTTAAATCATTA+GGG + chr7_4:54527462-54527481 Msa1163330:intron 15.0%
!! TAAATGTAAAATGACATAAA+AGG + chr7_4:54527811-54527830 Msa1163330:three_prime_UTR 15.0%
!! AAATGTAAAATGACATAAAA+GGG + chr7_4:54527812-54527831 Msa1163330:three_prime_UTR 15.0%
!!! AATCATATTTAATTTTGTGA+GGG - chr7_4:54526561-54526580 None:intergenic 15.0%
!! AAATAATAAAGACAGTAATT+GGG - chr7_4:54526590-54526609 None:intergenic 15.0%
!! AAAATAATAAAGACAGTAAT+TGG - chr7_4:54526591-54526610 None:intergenic 15.0%
!! TGAATGATTTATTAAAAGAT+GGG - chr7_4:54526737-54526756 None:intergenic 15.0%
!! TTGAATGATTTATTAAAAGA+TGG - chr7_4:54526738-54526757 None:intergenic 15.0%
!!! GGAAATTAGTTTTTTAATTA+AGG + chr7_4:54527139-54527158 Msa1163330:intron 15.0%
!!! TGAATCTTTTTAAATCATTA+GGG + chr7_4:54527462-54527481 Msa1163330:intron 15.0%
!! TAAATGTAAAATGACATAAA+AGG + chr7_4:54527811-54527830 Msa1163330:three_prime_UTR 15.0%
!! AAATGTAAAATGACATAAAA+GGG + chr7_4:54527812-54527831 Msa1163330:three_prime_UTR 15.0%
!! AATTGTAATAAAGGAAAAAC+TGG - chr7_4:54526534-54526553 None:intergenic 20.0%
!!! CAATCATATTTAATTTTGTG+AGG - chr7_4:54526562-54526581 None:intergenic 20.0%
!!! TTTTTTTGTTAAGAAACTCA+AGG - chr7_4:54526694-54526713 None:intergenic 20.0%
!!! TGTTTACATATTTTTGTTTG+TGG + chr7_4:54527118-54527137 Msa1163330:intron 20.0%
!!! TTTTGATTGTTGTAAATATC+AGG - chr7_4:54527211-54527230 None:intergenic 20.0%
!!! AAATAATAGCTTGCTAATTA+AGG + chr7_4:54527362-54527381 Msa1163330:intron 20.0%
!! AGATTCAGTTACAAATAAAA+AGG - chr7_4:54527449-54527468 None:intergenic 20.0%
!!! CTGAATCTTTTTAAATCATT+AGG + chr7_4:54527461-54527480 Msa1163330:intron 20.0%
!!! TTTTTAAATCATTAGGGATT+TGG + chr7_4:54527468-54527487 Msa1163330:intron 20.0%
!! AATTGTAATAAAGGAAAAAC+TGG - chr7_4:54526534-54526553 None:intergenic 20.0%
!!! CAATCATATTTAATTTTGTG+AGG - chr7_4:54526562-54526581 None:intergenic 20.0%
!!! TTTTTTTGTTAAGAAACTCA+AGG - chr7_4:54526694-54526713 None:intergenic 20.0%
!!! TGTTTACATATTTTTGTTTG+TGG + chr7_4:54527118-54527137 Msa1163330:intron 20.0%
!!! TTTTGATTGTTGTAAATATC+AGG - chr7_4:54527211-54527230 None:intergenic 20.0%
!!! AAATAATAGCTTGCTAATTA+AGG + chr7_4:54527362-54527381 Msa1163330:intron 20.0%
!! AGATTCAGTTACAAATAAAA+AGG - chr7_4:54527449-54527468 None:intergenic 20.0%
!!! CTGAATCTTTTTAAATCATT+AGG + chr7_4:54527461-54527480 Msa1163330:intron 20.0%
!!! TTTTTAAATCATTAGGGATT+TGG + chr7_4:54527468-54527487 Msa1163330:intron 20.0%
!! GCTTTCATACAATGTATTTT+GGG - chr7_4:54527268-54527287 None:intergenic 25.0%
!! TGCTTTCATACAATGTATTT+TGG - chr7_4:54527269-54527288 None:intergenic 25.0%
! TATGTAAATATCAGATGTTG+CGG + chr7_4:54527305-54527324 Msa1163330:intron 25.0%
! TTCAGTTACAAATAAAAAGG+AGG - chr7_4:54527446-54527465 None:intergenic 25.0%
!! GAAGTGTTTGGTAAAATTAA+CGG + chr7_4:54527775-54527794 Msa1163330:three_prime_UTR 25.0%
!! GCTTTCATACAATGTATTTT+GGG - chr7_4:54527268-54527287 None:intergenic 25.0%
!! TGCTTTCATACAATGTATTT+TGG - chr7_4:54527269-54527288 None:intergenic 25.0%
! TATGTAAATATCAGATGTTG+CGG + chr7_4:54527305-54527324 Msa1163330:intron 25.0%
! TTCAGTTACAAATAAAAAGG+AGG - chr7_4:54527446-54527465 None:intergenic 25.0%
!! GAAGTGTTTGGTAAAATTAA+CGG + chr7_4:54527775-54527794 Msa1163330:three_prime_UTR 25.0%
GAGGGTGAAAATTGTAATAA+AGG - chr7_4:54526543-54526562 None:intergenic 30.0%
ATGAGTGAAAAGTAGAAGAT+GGG - chr7_4:54526781-54526800 None:intergenic 30.0%
! TCAAAGATCTTTGTTTGTTG+AGG - chr7_4:54526804-54526823 None:intergenic 30.0%
!! GATTTCTGAATTGATTTGTC+TGG - chr7_4:54526880-54526899 None:intergenic 30.0%
!! TACATTTGATTTGACAACAG+CGG + chr7_4:54527597-54527616 Msa1163330:CDS 30.0%
GATTTAGGTGAAATAGACTT+CGG + chr7_4:54527634-54527653 Msa1163330:CDS 30.0%
GAGGGTGAAAATTGTAATAA+AGG - chr7_4:54526543-54526562 None:intergenic 30.0%
ATGAGTGAAAAGTAGAAGAT+GGG - chr7_4:54526781-54526800 None:intergenic 30.0%
! TCAAAGATCTTTGTTTGTTG+AGG - chr7_4:54526804-54526823 None:intergenic 30.0%
!! GATTTCTGAATTGATTTGTC+TGG - chr7_4:54526880-54526899 None:intergenic 30.0%
!! TACATTTGATTTGACAACAG+CGG + chr7_4:54527597-54527616 Msa1163330:CDS 30.0%
GATTTAGGTGAAATAGACTT+CGG + chr7_4:54527634-54527653 Msa1163330:CDS 30.0%
GATGAGTGAAAAGTAGAAGA+TGG - chr7_4:54526782-54526801 None:intergenic 35.0%
CTGATGTCGCTAAATAGATT+TGG - chr7_4:54526938-54526957 None:intergenic 35.0%
GACATCAGAAACCAAATCTA+CGG + chr7_4:54526950-54526969 Msa1163330:five_prime_UTR 35.0%
! TTTTCTTGCTCGCAATGAAA+AGG - chr7_4:54527046-54527065 None:intergenic 35.0%
TTTCATTGCGAGCAAGAAAA+TGG + chr7_4:54527046-54527065 Msa1163330:CDS 35.0%
TACCAAATCCAACAATCGAA+AGG + chr7_4:54527071-54527090 Msa1163330:CDS 35.0%
TTAAGGCTTAAAGCAATGCT+AGG + chr7_4:54527156-54527175 Msa1163330:intron 35.0%
GATTTGGAAGAAATTTCGCT+GGG + chr7_4:54527484-54527503 Msa1163330:CDS 35.0%
TACGGAAAATTCATCTGACA+CGG + chr7_4:54527507-54527526 Msa1163330:CDS 35.0%
ACTGTTGAAGAAACTGTTGA+TGG + chr7_4:54527559-54527578 Msa1163330:CDS 35.0%
AAATCAAATGTACATCCACC+AGG - chr7_4:54527589-54527608 None:intergenic 35.0%
GCTCTGTTTGGTAAAAATAG+CGG + chr7_4:54527656-54527675 Msa1163330:exon 35.0%
! GATAAGCTAGTTGAAGTGTT+TGG + chr7_4:54527763-54527782 Msa1163330:three_prime_UTR 35.0%
GATGAGTGAAAAGTAGAAGA+TGG - chr7_4:54526782-54526801 None:intergenic 35.0%
CTGATGTCGCTAAATAGATT+TGG - chr7_4:54526938-54526957 None:intergenic 35.0%
GACATCAGAAACCAAATCTA+CGG + chr7_4:54526950-54526969 Msa1163330:five_prime_UTR 35.0%
! TTTTCTTGCTCGCAATGAAA+AGG - chr7_4:54527046-54527065 None:intergenic 35.0%
TTTCATTGCGAGCAAGAAAA+TGG + chr7_4:54527046-54527065 Msa1163330:CDS 35.0%
TACCAAATCCAACAATCGAA+AGG + chr7_4:54527071-54527090 Msa1163330:CDS 35.0%
TTAAGGCTTAAAGCAATGCT+AGG + chr7_4:54527156-54527175 Msa1163330:intron 35.0%
GATTTGGAAGAAATTTCGCT+GGG + chr7_4:54527484-54527503 Msa1163330:CDS 35.0%
TACGGAAAATTCATCTGACA+CGG + chr7_4:54527507-54527526 Msa1163330:CDS 35.0%
ACTGTTGAAGAAACTGTTGA+TGG + chr7_4:54527559-54527578 Msa1163330:CDS 35.0%
AAATCAAATGTACATCCACC+AGG - chr7_4:54527589-54527608 None:intergenic 35.0%
GCTCTGTTTGGTAAAAATAG+CGG + chr7_4:54527656-54527675 Msa1163330:exon 35.0%
! GATAAGCTAGTTGAAGTGTT+TGG + chr7_4:54527763-54527782 Msa1163330:three_prime_UTR 35.0%
CTCAAGGATCTTCATTTGCT+GGG - chr7_4:54526678-54526697 None:intergenic 40.0%
ACTCAAGGATCTTCATTTGC+TGG - chr7_4:54526679-54526698 None:intergenic 40.0%
GTCTGGATCTTTGTTAGAGA+AGG - chr7_4:54526863-54526882 None:intergenic 40.0%
! AAATAGATTTGGTTCGCCGT+AGG - chr7_4:54526927-54526946 None:intergenic 40.0%
TTGAAAGTGGTAGACGACTA+TGG + chr7_4:54527006-54527025 Msa1163330:exon 40.0%
GTCCTTTCGATTGTTGGATT+TGG - chr7_4:54527076-54527095 None:intergenic 40.0%
GGATTTGGAAGAAATTTCGC+TGG + chr7_4:54527483-54527502 Msa1163330:CDS 40.0%
! GTGAACGGTTTGGATGATTT+AGG + chr7_4:54527619-54527638 Msa1163330:CDS 40.0%
AAATAGACTTCGGCTCTGTT+TGG + chr7_4:54527644-54527663 Msa1163330:CDS 40.0%
CTCAAGGATCTTCATTTGCT+GGG - chr7_4:54526678-54526697 None:intergenic 40.0%
ACTCAAGGATCTTCATTTGC+TGG - chr7_4:54526679-54526698 None:intergenic 40.0%
GTCTGGATCTTTGTTAGAGA+AGG - chr7_4:54526863-54526882 None:intergenic 40.0%
! AAATAGATTTGGTTCGCCGT+AGG - chr7_4:54526927-54526946 None:intergenic 40.0%
TTGAAAGTGGTAGACGACTA+TGG + chr7_4:54527006-54527025 Msa1163330:exon 40.0%
GTCCTTTCGATTGTTGGATT+TGG - chr7_4:54527076-54527095 None:intergenic 40.0%
GGATTTGGAAGAAATTTCGC+TGG + chr7_4:54527483-54527502 Msa1163330:CDS 40.0%
! GTGAACGGTTTGGATGATTT+AGG + chr7_4:54527619-54527638 Msa1163330:CDS 40.0%
AAATAGACTTCGGCTCTGTT+TGG + chr7_4:54527644-54527663 Msa1163330:CDS 40.0%
! ATTTGCTGGGTTACAGTCTC+GGG - chr7_4:54526665-54526684 None:intergenic 45.0%
! CATTTGCTGGGTTACAGTCT+CGG - chr7_4:54526666-54526685 None:intergenic 45.0%
ATCTGAGGAGCTGTTGAAAG+TGG + chr7_4:54526993-54527012 Msa1163330:five_prime_UTR 45.0%
TCCAACAATCGAAAGGACAG+AGG + chr7_4:54527078-54527097 Msa1163330:CDS 45.0%
ACCTCTGTCCTTTCGATTGT+TGG - chr7_4:54527082-54527101 None:intergenic 45.0%
GGAAGAAATTTCGCTGGGTA+CGG + chr7_4:54527489-54527508 Msa1163330:CDS 45.0%
GATTTGACAACAGCGGTGAA+CGG + chr7_4:54527604-54527623 Msa1163330:CDS 45.0%
! ATTTGCTGGGTTACAGTCTC+GGG - chr7_4:54526665-54526684 None:intergenic 45.0%
! CATTTGCTGGGTTACAGTCT+CGG - chr7_4:54526666-54526685 None:intergenic 45.0%
ATCTGAGGAGCTGTTGAAAG+TGG + chr7_4:54526993-54527012 Msa1163330:five_prime_UTR 45.0%
TCCAACAATCGAAAGGACAG+AGG + chr7_4:54527078-54527097 Msa1163330:CDS 45.0%
ACCTCTGTCCTTTCGATTGT+TGG - chr7_4:54527082-54527101 None:intergenic 45.0%
GGAAGAAATTTCGCTGGGTA+CGG + chr7_4:54527489-54527508 Msa1163330:CDS 45.0%
GATTTGACAACAGCGGTGAA+CGG + chr7_4:54527604-54527623 Msa1163330:CDS 45.0%
TGGGTTACAGTCTCGGGATT+TGG - chr7_4:54526659-54526678 None:intergenic 50.0%
!! GAAACTGTTGATGGTGTGCC+TGG + chr7_4:54527568-54527587 Msa1163330:CDS 50.0%
TGGGTTACAGTCTCGGGATT+TGG - chr7_4:54526659-54526678 None:intergenic 50.0%
!! GAAACTGTTGATGGTGTGCC+TGG + chr7_4:54527568-54527587 Msa1163330:CDS 50.0%
CAGTACCGTCGTCGAACCTA+CGG + chr7_4:54526908-54526927 Msa1163330:five_prime_UTR 55.0%
CGGAAGGTTCGCCGTAGATT+TGG - chr7_4:54526964-54526983 None:intergenic 55.0%
CTCAGATTTGCGACGACGGA+AGG - chr7_4:54526980-54526999 None:intergenic 55.0%
CTTCCGTCGTCGCAAATCTG+AGG + chr7_4:54526978-54526997 Msa1163330:five_prime_UTR 55.0%
GCTCCTCAGATTTGCGACGA+CGG - chr7_4:54526984-54527003 None:intergenic 55.0%
!! ACTGTTGATGGTGTGCCTGG+TGG + chr7_4:54527571-54527590 Msa1163330:CDS 55.0%
GACAACAGCGGTGAACGGTT+TGG + chr7_4:54527609-54527628 Msa1163330:CDS 55.0%
CAGTACCGTCGTCGAACCTA+CGG + chr7_4:54526908-54526927 Msa1163330:five_prime_UTR 55.0%
CGGAAGGTTCGCCGTAGATT+TGG - chr7_4:54526964-54526983 None:intergenic 55.0%
CTCAGATTTGCGACGACGGA+AGG - chr7_4:54526980-54526999 None:intergenic 55.0%
CTTCCGTCGTCGCAAATCTG+AGG + chr7_4:54526978-54526997 Msa1163330:five_prime_UTR 55.0%
GCTCCTCAGATTTGCGACGA+CGG - chr7_4:54526984-54527003 None:intergenic 55.0%
!! ACTGTTGATGGTGTGCCTGG+TGG + chr7_4:54527571-54527590 Msa1163330:CDS 55.0%
GACAACAGCGGTGAACGGTT+TGG + chr7_4:54527609-54527628 Msa1163330:CDS 55.0%
GGTTCGACGACGGTACTGCA+AGG - chr7_4:54526906-54526925 None:intergenic 60.0%
GTTCGCCGTAGGTTCGACGA+CGG - chr7_4:54526916-54526935 None:intergenic 60.0%
GGTTCGACGACGGTACTGCA+AGG - chr7_4:54526906-54526925 None:intergenic 60.0%
GTTCGCCGTAGGTTCGACGA+CGG - chr7_4:54526916-54526935 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 54526522 54527836 54526522 ID=Msa1163330;Name=Msa1163330
chr7_4 mRNA 54526522 54527836 54526522 ID=Msa1163330-mRNA-1;Parent=Msa1163330;Name=Msa1163330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=503|0|0.66|0.66|0|0.66|3|167|100
chr7_4 exon 54526522 54527099 54526522 ID=Msa1163330-mRNA-1:exon:15832;Parent=Msa1163330-mRNA-1
chr7_4 exon 54527197 54527237 54527197 ID=Msa1163330-mRNA-1:exon:15833;Parent=Msa1163330-mRNA-1
chr7_4 exon 54527483 54527836 54527483 ID=Msa1163330-mRNA-1:exon:15834;Parent=Msa1163330-mRNA-1
chr7_4 five_prime_UTR 54526522 54527024 54526522 ID=Msa1163330-mRNA-1:five_prime_utr;Parent=Msa1163330-mRNA-1
chr7_4 CDS 54527025 54527099 54527025 ID=Msa1163330-mRNA-1:cds;Parent=Msa1163330-mRNA-1
chr7_4 CDS 54527197 54527237 54527197 ID=Msa1163330-mRNA-1:cds;Parent=Msa1163330-mRNA-1
chr7_4 CDS 54527483 54527669 54527483 ID=Msa1163330-mRNA-1:cds;Parent=Msa1163330-mRNA-1
chr7_4 three_prime_UTR 54527670 54527836 54527670 ID=Msa1163330-mRNA-1:three_prime_utr;Parent=Msa1163330-mRNA-1
Gene Sequence

>Msa1163330

ATGGCTGATGCTGAATTTCCTTTTCATTGCGAGCAAGAAAATGGTGTACCAAATCCAACAATCGAAAGGACAGAGAGCTTAAAGATCCTGATATTTACAACAATCAAAATTTTTTTGGATTTGGAAGAAATTTCGCTGGGTACGGAAAATTCATCTGACACGGATGAGTCTGTTAGAGAAAATGTTGAAGAAACTGTTGAAGAAACTGTTGATGGTGTGCCTGGTGGATGTACATTTGATTTGACAACAGCGGTGAACGGTTTGGATGATTTAGGTGAAATAGACTTCGGCTCTGTTTGGTAA
Protein sequence

>Msa1163330

MADAEFPFHCEQENGVPNPTIERTESLKILIFTTIKIFLDLEEISLGTENSSDTDESVRENVEETVEETVDGVPGGCTFDLTTAVNGLDDLGEIDFGSVW*