Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0084440 | Msa0213670 | 0.806299 | 8.801740e-50 | -8.615850e-47 |
| Msa0084440 | Msa0248130 | 0.809706 | 1.657242e-50 | -8.615850e-47 |
| Msa0084440 | Msa0269940 | 0.807512 | 4.876156e-50 | -8.615850e-47 |
| Msa0084440 | Msa0319860 | 0.815318 | 9.832131e-52 | -8.615850e-47 |
| Msa0084440 | Msa0353620 | 0.801006 | 1.103498e-48 | -8.615850e-47 |
| Msa0084440 | Msa0393570 | 0.861723 | 8.394785e-64 | -8.615850e-47 |
| Msa0084440 | Msa0393590 | 0.873596 | 1.300764e-67 | -8.615850e-47 |
| Msa0084440 | Msa0737340 | 0.813349 | 2.677609e-51 | -8.615850e-47 |
| Msa0084440 | Msa0890270 | 0.810024 | 1.415507e-50 | -8.615850e-47 |
| Msa0084440 | Msa0942260 | 0.809939 | 1.476762e-50 | -8.615850e-47 |
| Msa0084440 | Msa1091980 | 0.808973 | 2.379842e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGCTTCTGTATCATATAA+AGG | 0.283424 | 1_2:-63882139 | None:intergenic |
| AGGCCACGATTTGTAGCTTT+TGG | 0.323699 | 1_2:+63881735 | Msa0084440:CDS |
| ACCAACAATGTCTGCAAACT+TGG | 0.361386 | 1_2:-63881940 | None:intergenic |
| TCTGCAAACTTGGTGAGCTT+TGG | 0.374688 | 1_2:-63881930 | None:intergenic |
| TATTCTATCATCCATGTACT+AGG | 0.388972 | 1_2:+63881513 | Msa0084440:CDS |
| GTGCTTCTGTATCATATAAA+GGG | 0.427543 | 1_2:-63882138 | None:intergenic |
| AAACAAACCTTTGGATGCCA+TGG | 0.474075 | 1_2:-63881815 | None:intergenic |
| ACCAAGTTTGCAGACATTGT+TGG | 0.493260 | 1_2:+63881939 | Msa0084440:CDS |
| TTGCAGACATTGTTGGTGGA+AGG | 0.522383 | 1_2:+63881946 | Msa0084440:CDS |
| CTTGCTGCATGCCTAGTACA+TGG | 0.527464 | 1_2:-63881524 | None:intergenic |
| AGGATTGACTTCCAGATCCA+AGG | 0.534893 | 1_2:+63881966 | Msa0084440:CDS |
| GGATGATAGAATAAATTATG+AGG | 0.554822 | 1_2:-63881503 | None:intergenic |
| AAGTTTGCAGACATTGTTGG+TGG | 0.561560 | 1_2:+63881942 | Msa0084440:CDS |
| TACTGCTTAGGAACAGATGC+AGG | 0.569954 | 1_2:+63881467 | None:intergenic |
| ATACAGGCCACGATTTGTAC+AGG | 0.577844 | 1_2:+63881715 | Msa0084440:intron |
| AACAAATCCATGGCATCCAA+AGG | 0.609245 | 1_2:+63881808 | Msa0084440:CDS |
| ATCGTGGCCTGTACAAATCG+TGG | 0.635831 | 1_2:-63881722 | None:intergenic |
| TGACCAAAAGCTACAAATCG+TGG | 0.645101 | 1_2:-63881738 | None:intergenic |
| TATCATATAAAGGGACAACA+TGG | 0.646389 | 1_2:-63882129 | None:intergenic |
| AGCAGTGGCTCGTTTATCTG+AGG | 0.648058 | 1_2:-63881549 | None:intergenic |
| AAAGGATGGTCACATAGCAG+TGG | 0.677759 | 1_2:-63881564 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTAAAATTATAAAAACCT+TGG | - | chr1_2:63881986-63882005 | None:intergenic | 15.0% |
| !!! | TATAATTTTAAAGTCATTGA+TGG | + | chr1_2:63881993-63882012 | Msa0084440:intron | 15.0% |
| !!! | ATAATTTTAAAGTCATTGAT+GGG | + | chr1_2:63881994-63882013 | Msa0084440:intron | 15.0% |
| !!! | CTTTAAAATTATAAAAACCT+TGG | - | chr1_2:63881986-63882005 | None:intergenic | 15.0% |
| !!! | TATAATTTTAAAGTCATTGA+TGG | + | chr1_2:63881993-63882012 | Msa0084440:intron | 15.0% |
| !!! | ATAATTTTAAAGTCATTGAT+GGG | + | chr1_2:63881994-63882013 | Msa0084440:intron | 15.0% |
| !!! | AATAAATGTTTTTGAAAGCA+TGG | - | chr1_2:63881601-63881620 | None:intergenic | 20.0% |
| !! | TATGACAACAAATATGAAAT+TGG | - | chr1_2:63881848-63881867 | None:intergenic | 20.0% |
| !! | ATCCTTCAAAAAAAAAAACA+GGG | - | chr1_2:63882101-63882120 | None:intergenic | 20.0% |
| !!! | AAGACTTCATTTTATTCATT+TGG | + | chr1_2:63882202-63882221 | Msa0084440:CDS | 20.0% |
| !!! | AATAAATGTTTTTGAAAGCA+TGG | - | chr1_2:63881601-63881620 | None:intergenic | 20.0% |
| !! | TATGACAACAAATATGAAAT+TGG | - | chr1_2:63881848-63881867 | None:intergenic | 20.0% |
| !! | ATCCTTCAAAAAAAAAAACA+GGG | - | chr1_2:63882101-63882120 | None:intergenic | 20.0% |
| !!! | AAGACTTCATTTTATTCATT+TGG | + | chr1_2:63882202-63882221 | Msa0084440:CDS | 20.0% |
| ! | GGATGATAGAATAAATTATG+AGG | - | chr1_2:63881506-63881525 | None:intergenic | 25.0% |
| ! | AATTATAAAAACCTTGGATC+TGG | - | chr1_2:63881980-63881999 | None:intergenic | 25.0% |
| !!! | TACCCTGTTTTTTTTTTTGA+AGG | + | chr1_2:63882096-63882115 | Msa0084440:intron | 25.0% |
| ! | CATCCTTCAAAAAAAAAAAC+AGG | - | chr1_2:63882102-63882121 | None:intergenic | 25.0% |
| !!! | TTTGGTTCTCTAATTTTCTT+TGG | + | chr1_2:63882220-63882239 | Msa0084440:CDS | 25.0% |
| ! | GGATGATAGAATAAATTATG+AGG | - | chr1_2:63881506-63881525 | None:intergenic | 25.0% |
| ! | AATTATAAAAACCTTGGATC+TGG | - | chr1_2:63881980-63881999 | None:intergenic | 25.0% |
| !!! | TACCCTGTTTTTTTTTTTGA+AGG | + | chr1_2:63882096-63882115 | Msa0084440:intron | 25.0% |
| ! | CATCCTTCAAAAAAAAAAAC+AGG | - | chr1_2:63882102-63882121 | None:intergenic | 25.0% |
| !!! | TTTGGTTCTCTAATTTTCTT+TGG | + | chr1_2:63882220-63882239 | Msa0084440:CDS | 25.0% |
| TATTCTATCATCCATGTACT+AGG | + | chr1_2:63881513-63881532 | Msa0084440:CDS | 30.0% | |
| TGTTCAAGCACTATAAATAC+AGG | + | chr1_2:63881699-63881718 | Msa0084440:intron | 30.0% | |
| GGAATTAAGAAACAAACCTT+TGG | - | chr1_2:63881827-63881846 | None:intergenic | 30.0% | |
| ! | GAATATGTAAGAGTGTGAAA+GGG | + | chr1_2:63882016-63882035 | Msa0084440:intron | 30.0% |
| TATCATATAAAGGGACAACA+TGG | - | chr1_2:63882132-63882151 | None:intergenic | 30.0% | |
| GTGCTTCTGTATCATATAAA+GGG | - | chr1_2:63882141-63882160 | None:intergenic | 30.0% | |
| TGTGCTTCTGTATCATATAA+AGG | - | chr1_2:63882142-63882161 | None:intergenic | 30.0% | |
| TATTCTATCATCCATGTACT+AGG | + | chr1_2:63881513-63881532 | Msa0084440:CDS | 30.0% | |
| TGTTCAAGCACTATAAATAC+AGG | + | chr1_2:63881699-63881718 | Msa0084440:intron | 30.0% | |
| GGAATTAAGAAACAAACCTT+TGG | - | chr1_2:63881827-63881846 | None:intergenic | 30.0% | |
| ! | GAATATGTAAGAGTGTGAAA+GGG | + | chr1_2:63882016-63882035 | Msa0084440:intron | 30.0% |
| TATCATATAAAGGGACAACA+TGG | - | chr1_2:63882132-63882151 | None:intergenic | 30.0% | |
| GTGCTTCTGTATCATATAAA+GGG | - | chr1_2:63882141-63882160 | None:intergenic | 30.0% | |
| TGTGCTTCTGTATCATATAA+AGG | - | chr1_2:63882142-63882161 | None:intergenic | 30.0% | |
| TATGTGACCATCCTTTGTTA+TGG | + | chr1_2:63881571-63881590 | Msa0084440:intron | 35.0% | |
| ACAATAGCGCATGAAATTCT+TGG | - | chr1_2:63881655-63881674 | None:intergenic | 35.0% | |
| TATAGTGCTTGAACAACATC+TGG | - | chr1_2:63881694-63881713 | None:intergenic | 35.0% | |
| ! | GTTTTGCTGAAACAAATCCA+TGG | + | chr1_2:63881798-63881817 | Msa0084440:CDS | 35.0% |
| TAACCAGTTCTTTCTAATGC+AGG | + | chr1_2:63881900-63881919 | Msa0084440:intron | 35.0% | |
| TTTCCTGCATTAGAAAGAAC+TGG | - | chr1_2:63881906-63881925 | None:intergenic | 35.0% | |
| ! | GGAATATGTAAGAGTGTGAA+AGG | + | chr1_2:63882015-63882034 | Msa0084440:intron | 35.0% |
| TATGTGACCATCCTTTGTTA+TGG | + | chr1_2:63881571-63881590 | Msa0084440:intron | 35.0% | |
| ACAATAGCGCATGAAATTCT+TGG | - | chr1_2:63881655-63881674 | None:intergenic | 35.0% | |
| TATAGTGCTTGAACAACATC+TGG | - | chr1_2:63881694-63881713 | None:intergenic | 35.0% | |
| ! | GTTTTGCTGAAACAAATCCA+TGG | + | chr1_2:63881798-63881817 | Msa0084440:CDS | 35.0% |
| TAACCAGTTCTTTCTAATGC+AGG | + | chr1_2:63881900-63881919 | Msa0084440:intron | 35.0% | |
| TTTCCTGCATTAGAAAGAAC+TGG | - | chr1_2:63881906-63881925 | None:intergenic | 35.0% | |
| ! | GGAATATGTAAGAGTGTGAA+AGG | + | chr1_2:63882015-63882034 | Msa0084440:intron | 35.0% |
| TGGCTAACCATAACAAAGGA+TGG | - | chr1_2:63881581-63881600 | None:intergenic | 40.0% | |
| AGCATGGCTAACCATAACAA+AGG | - | chr1_2:63881585-63881604 | None:intergenic | 40.0% | |
| ATTCTTGGAGGTTGAGTACA+GGG | - | chr1_2:63881640-63881659 | None:intergenic | 40.0% | |
| AATTCTTGGAGGTTGAGTAC+AGG | - | chr1_2:63881641-63881660 | None:intergenic | 40.0% | |
| ATAGCGCATGAAATTCTTGG+AGG | - | chr1_2:63881652-63881671 | None:intergenic | 40.0% | |
| TGACCAAAAGCTACAAATCG+TGG | - | chr1_2:63881741-63881760 | None:intergenic | 40.0% | |
| AACAAATCCATGGCATCCAA+AGG | + | chr1_2:63881808-63881827 | Msa0084440:CDS | 40.0% | |
| AAACAAACCTTTGGATGCCA+TGG | - | chr1_2:63881818-63881837 | None:intergenic | 40.0% | |
| ACCAAGTTTGCAGACATTGT+TGG | + | chr1_2:63881939-63881958 | Msa0084440:CDS | 40.0% | |
| ACCAACAATGTCTGCAAACT+TGG | - | chr1_2:63881943-63881962 | None:intergenic | 40.0% | |
| AAGTTTGCAGACATTGTTGG+TGG | + | chr1_2:63881942-63881961 | Msa0084440:CDS | 40.0% | |
| TACCTTACACATGCTGATCA+TGG | + | chr1_2:63882056-63882075 | Msa0084440:intron | 40.0% | |
| ACCTTACACATGCTGATCAT+GGG | + | chr1_2:63882057-63882076 | Msa0084440:intron | 40.0% | |
| ACATGCTGATCATGGGTATA+AGG | + | chr1_2:63882064-63882083 | Msa0084440:intron | 40.0% | |
| TGGCTAACCATAACAAAGGA+TGG | - | chr1_2:63881581-63881600 | None:intergenic | 40.0% | |
| AGCATGGCTAACCATAACAA+AGG | - | chr1_2:63881585-63881604 | None:intergenic | 40.0% | |
| ATTCTTGGAGGTTGAGTACA+GGG | - | chr1_2:63881640-63881659 | None:intergenic | 40.0% | |
| AATTCTTGGAGGTTGAGTAC+AGG | - | chr1_2:63881641-63881660 | None:intergenic | 40.0% | |
| ATAGCGCATGAAATTCTTGG+AGG | - | chr1_2:63881652-63881671 | None:intergenic | 40.0% | |
| TGACCAAAAGCTACAAATCG+TGG | - | chr1_2:63881741-63881760 | None:intergenic | 40.0% | |
| AACAAATCCATGGCATCCAA+AGG | + | chr1_2:63881808-63881827 | Msa0084440:CDS | 40.0% | |
| AAACAAACCTTTGGATGCCA+TGG | - | chr1_2:63881818-63881837 | None:intergenic | 40.0% | |
| ACCAAGTTTGCAGACATTGT+TGG | + | chr1_2:63881939-63881958 | Msa0084440:CDS | 40.0% | |
| ACCAACAATGTCTGCAAACT+TGG | - | chr1_2:63881943-63881962 | None:intergenic | 40.0% | |
| AAGTTTGCAGACATTGTTGG+TGG | + | chr1_2:63881942-63881961 | Msa0084440:CDS | 40.0% | |
| TACCTTACACATGCTGATCA+TGG | + | chr1_2:63882056-63882075 | Msa0084440:intron | 40.0% | |
| ACCTTACACATGCTGATCAT+GGG | + | chr1_2:63882057-63882076 | Msa0084440:intron | 40.0% | |
| ACATGCTGATCATGGGTATA+AGG | + | chr1_2:63882064-63882083 | Msa0084440:intron | 40.0% | |
| AAAGGATGGTCACATAGCAG+TGG | - | chr1_2:63881567-63881586 | None:intergenic | 45.0% | |
| ATACAGGCCACGATTTGTAC+AGG | + | chr1_2:63881715-63881734 | Msa0084440:intron | 45.0% | |
| ! | AGGCCACGATTTGTAGCTTT+TGG | + | chr1_2:63881735-63881754 | Msa0084440:CDS | 45.0% |
| ! | TCTGCAAACTTGGTGAGCTT+TGG | - | chr1_2:63881933-63881952 | None:intergenic | 45.0% |
| ! | TTGCAGACATTGTTGGTGGA+AGG | + | chr1_2:63881946-63881965 | Msa0084440:CDS | 45.0% |
| ! | AGGATTGACTTCCAGATCCA+AGG | + | chr1_2:63881966-63881985 | Msa0084440:CDS | 45.0% |
| ACCCATGATCAGCATGTGTA+AGG | - | chr1_2:63882061-63882080 | None:intergenic | 45.0% | |
| AAAGGATGGTCACATAGCAG+TGG | - | chr1_2:63881567-63881586 | None:intergenic | 45.0% | |
| ATACAGGCCACGATTTGTAC+AGG | + | chr1_2:63881715-63881734 | Msa0084440:intron | 45.0% | |
| ! | AGGCCACGATTTGTAGCTTT+TGG | + | chr1_2:63881735-63881754 | Msa0084440:CDS | 45.0% |
| ! | TCTGCAAACTTGGTGAGCTT+TGG | - | chr1_2:63881933-63881952 | None:intergenic | 45.0% |
| ! | TTGCAGACATTGTTGGTGGA+AGG | + | chr1_2:63881946-63881965 | Msa0084440:CDS | 45.0% |
| ! | AGGATTGACTTCCAGATCCA+AGG | + | chr1_2:63881966-63881985 | Msa0084440:CDS | 45.0% |
| ACCCATGATCAGCATGTGTA+AGG | - | chr1_2:63882061-63882080 | None:intergenic | 45.0% | |
| CTTGCTGCATGCCTAGTACA+TGG | - | chr1_2:63881527-63881546 | None:intergenic | 50.0% | |
| AGCAGTGGCTCGTTTATCTG+AGG | - | chr1_2:63881552-63881571 | None:intergenic | 50.0% | |
| TGAGTACAGGGATGGTTGAC+AGG | - | chr1_2:63881628-63881647 | None:intergenic | 50.0% | |
| TTGGAGGTTGAGTACAGGGA+TGG | - | chr1_2:63881636-63881655 | None:intergenic | 50.0% | |
| ATCGTGGCCTGTACAAATCG+TGG | - | chr1_2:63881725-63881744 | None:intergenic | 50.0% | |
| CTTGCTGCATGCCTAGTACA+TGG | - | chr1_2:63881527-63881546 | None:intergenic | 50.0% | |
| AGCAGTGGCTCGTTTATCTG+AGG | - | chr1_2:63881552-63881571 | None:intergenic | 50.0% | |
| TGAGTACAGGGATGGTTGAC+AGG | - | chr1_2:63881628-63881647 | None:intergenic | 50.0% | |
| TTGGAGGTTGAGTACAGGGA+TGG | - | chr1_2:63881636-63881655 | None:intergenic | 50.0% | |
| ATCGTGGCCTGTACAAATCG+TGG | - | chr1_2:63881725-63881744 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 63881477 | 63882267 | 63881477 | ID=Msa0084440;Name=Msa0084440 |
| chr1_2 | mRNA | 63881477 | 63882267 | 63881477 | ID=Msa0084440-mRNA-1;Parent=Msa0084440;Name=Msa0084440-mRNA-1;_AED=0.31;_eAED=0.31;_QI=14|0.33|0|1|0.33|0.5|4|0|136 |
| chr1_2 | exon | 63881477 | 63881573 | 63881477 | ID=Msa0084440-mRNA-1:exon:21420;Parent=Msa0084440-mRNA-1 |
| chr1_2 | exon | 63881721 | 63881829 | 63881721 | ID=Msa0084440-mRNA-1:exon:21421;Parent=Msa0084440-mRNA-1 |
| chr1_2 | exon | 63881922 | 63881987 | 63881922 | ID=Msa0084440-mRNA-1:exon:21422;Parent=Msa0084440-mRNA-1 |
| chr1_2 | exon | 63882118 | 63882267 | 63882118 | ID=Msa0084440-mRNA-1:exon:21423;Parent=Msa0084440-mRNA-1 |
| chr1_2 | five_prime_UTR | 63881477 | 63881490 | 63881477 | ID=Msa0084440-mRNA-1:five_prime_utr;Parent=Msa0084440-mRNA-1 |
| chr1_2 | CDS | 63881491 | 63881573 | 63881491 | ID=Msa0084440-mRNA-1:cds;Parent=Msa0084440-mRNA-1 |
| chr1_2 | CDS | 63881721 | 63881829 | 63881721 | ID=Msa0084440-mRNA-1:cds;Parent=Msa0084440-mRNA-1 |
| chr1_2 | CDS | 63881922 | 63881987 | 63881922 | ID=Msa0084440-mRNA-1:cds;Parent=Msa0084440-mRNA-1 |
| chr1_2 | CDS | 63882118 | 63882267 | 63882118 | ID=Msa0084440-mRNA-1:cds;Parent=Msa0084440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
