AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0897630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897630 MtrunA17_Chr6g0483441 91.837 294 5 1 1 275 1 294 0.0 549
Msa0897630 MtrunA17_Chr8g0392551 65.449 301 73 6 1 275 1 296 2.02e-113 328
Msa0897630 MtrunA17_Chr4g0053001 48.916 323 113 9 1 275 12 330 7.19e-86 259
Msa0897630 MtrunA17_Chr4g0038691 44.201 319 126 10 1 274 6 317 2.91e-67 211
Msa0897630 MtrunA17_Chr2g0331671 37.987 308 148 9 7 275 6 309 2.17e-57 186
Msa0897630 MtrunA17_Chr6g0486001 35.873 315 153 8 1 275 1 306 2.97e-50 167
Msa0897630 MtrunA17_Chr3g0091821 35.220 159 77 3 138 275 41 194 3.25e-24 96.7
Msa0897630 MtrunA17_Chr8g0379271 40.000 95 53 2 182 274 1 93 1.86e-14 67.8
Msa0897630 MtrunA17_Chr3g0090061 24.757 206 115 6 87 257 129 329 5.24e-12 65.1
Msa0897630 MtrunA17_Chr8g0379281 35.152 165 85 6 23 182 20 167 1.81e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897630 AT1G45688.1 46.064 343 110 9 1 273 1 338 1.77e-87 264
Msa0897630 AT5G42860.1 48.297 323 110 11 1 273 1 316 7.89e-83 251
Msa0897630 AT1G45688.2 53.302 212 64 7 1 182 1 207 2.56e-66 207
Msa0897630 AT4G35170.1 34.237 295 155 7 18 273 3 297 4.17e-48 162
Msa0897630 AT2G41990.1 35.065 308 151 8 1 272 1 295 1.07e-46 158
Msa0897630 AT3G24605.1 32.281 285 151 7 1 253 3 277 4.16e-35 128
Msa0897630 AT3G24600.1 30.272 294 158 8 8 261 3 289 2.85e-24 99.8

Find 80 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAACACTGGCACTTTCTT+TGG 0.173423 6_1:+89981994 Msa0897630:CDS
ATTAATTAATGTAATCAAAA+TGG 0.178124 6_1:-89983811 None:intergenic
AGCTTATGTACTTGGAAATT+TGG 0.181196 6_1:+89983400 Msa0897630:CDS
TGAATATTCATATATGTTAA+AGG 0.229177 6_1:+89983521 Msa0897630:exon
AGAGAAAGATGAGAATAATT+TGG 0.254028 6_1:-89980286 None:intergenic
TAATGGATTCCTTTGAGATA+TGG 0.280476 6_1:+89983605 Msa0897630:three_prime_UTR
ACTAGAGCACCAAGTTTCTT+AGG 0.281801 6_1:-89983470 None:intergenic
TGAAGCACAAATACTATAAA+AGG 0.290325 6_1:+89983423 Msa0897630:CDS
AGAGGAATGGTGGTTATTCT+AGG 0.308845 6_1:+89980760 Msa0897630:CDS
GAAAGTGTAAAAGAATTATT+AGG 0.332630 6_1:+89983558 Msa0897630:three_prime_UTR
ACAAACCTTGATCACAATCT+TGG 0.367265 6_1:-89980874 None:intergenic
ACTCAGAAGGCAAGATGATT+AGG 0.386791 6_1:+89983655 Msa0897630:three_prime_UTR
GAATTTCAAAGTAGAGTTCA+TGG 0.389229 6_1:-89981965 None:intergenic
GAATATTCATATATGTTAAA+GGG 0.389673 6_1:+89983522 Msa0897630:exon
ATCACAATCTTGGGCTTCAT+AGG 0.399696 6_1:-89980864 None:intergenic
TTTGTAGTTTGTGTTAGTTT+TGG 0.406206 6_1:-89980327 None:intergenic
TTGATCTCATCACAAAGTTA+AGG 0.410482 6_1:-89983376 None:intergenic
TGAGATATGGATGGAATTGA+TGG 0.423689 6_1:+89983618 Msa0897630:three_prime_UTR
AGTGGAAGAATCTGTACAAC+TGG 0.427781 6_1:-89980261 None:intergenic
GAAGCCCAAGATTGTGATCA+AGG 0.437262 6_1:+89980869 Msa0897630:CDS
ATTGATGTTATTGAAGAAGA+AGG 0.441499 6_1:+89980723 Msa0897630:CDS
GGTAGCAAGGTCCCTTTGTA+TGG 0.447350 6_1:+89983296 Msa0897630:CDS
TTAATTAATGTAATCAAAAT+GGG 0.450915 6_1:-89983810 None:intergenic
CAAACCTTGATCACAATCTT+GGG 0.451461 6_1:-89980873 None:intergenic
GCAAGCCTTAGCAGCACAAC+GGG 0.460597 6_1:+89983326 Msa0897630:CDS
AGATCAAGAGCTTATGTACT+TGG 0.464263 6_1:+89983392 Msa0897630:CDS
TGTTACTGTTCAAGCCGGTT+CGG 0.469493 6_1:+89981912 Msa0897630:CDS
CAAGCCGGTTCGGATTCGAC+TGG 0.472107 6_1:+89981922 Msa0897630:CDS
ACCTTGGATCCTAAGAAACT+TGG 0.478203 6_1:+89983461 Msa0897630:CDS
AAGTTAAGGTTCAATGGTAC+TGG 0.480825 6_1:-89983362 None:intergenic
GAATGATGAGGTGAAGCAAC+AGG 0.483777 6_1:-89980627 None:intergenic
ATCACAAAGTTAAGGTTCAA+TGG 0.484575 6_1:-89983368 None:intergenic
CACCCCAGTCGAATCCGAAC+CGG 0.486023 6_1:-89981926 None:intergenic
ACAAAGATCATGTGTTGGTT+CGG 0.486095 6_1:+89983709 Msa0897630:three_prime_UTR
TTGATGTTATTGAAGAAGAA+GGG 0.490404 6_1:+89980724 Msa0897630:CDS
ATCTAGGAAGAGTCATAGAT+TGG 0.493508 6_1:+89983256 Msa0897630:CDS
TTGGAGAAAGAGAGGAATGG+TGG 0.495776 6_1:+89980750 Msa0897630:CDS
TGAAGGAGTTTCATCAATCT+AGG 0.500553 6_1:+89983240 Msa0897630:CDS
ATTGGTGAGTGTGGCAGTGA+TGG 0.501754 6_1:+89983274 Msa0897630:CDS
ATAACATCAATATGCTTCCA+TGG 0.513867 6_1:-89980711 None:intergenic
GGACTTTGAACGTAGTATAG+TGG 0.522534 6_1:-89980558 None:intergenic
GCATACCCGTTGTGCTGCTA+AGG 0.522833 6_1:-89983331 None:intergenic
CGACGTGGTGGTGATCTTGT+TGG 0.523915 6_1:-89980534 None:intergenic
TTGCACCACTTCCATACAAA+GGG 0.535212 6_1:-89983307 None:intergenic
ACCAAGTTTCTTAGGATCCA+AGG 0.536037 6_1:-89983462 None:intergenic
GTGAAGCAACAGGTGTTGAA+TGG 0.539497 6_1:-89980617 None:intergenic
GATTCAATGTTCCATCACCT+TGG 0.548796 6_1:+89983445 Msa0897630:CDS
GATCATGTTACTGTTCAAGC+CGG 0.548899 6_1:+89981907 Msa0897630:CDS
ATCAACATAGTGTTAATCCA+TGG 0.550799 6_1:+89980694 Msa0897630:CDS
TTGGTGAGTGTGGCAGTGAT+GGG 0.550942 6_1:+89983275 Msa0897630:CDS
AAGGTCCCTTTGTATGGAAG+TGG 0.551894 6_1:+89983302 Msa0897630:CDS
AAATTCACATACCGCAACAC+TGG 0.552172 6_1:+89981982 Msa0897630:CDS
GAGATATGGATGGAATTGAT+GGG 0.559554 6_1:+89983619 Msa0897630:three_prime_UTR
TTTCTTAGGATCCAAGGTGA+TGG 0.560780 6_1:-89983456 None:intergenic
CATATGTGCAAGAATTCTTG+AGG 0.561648 6_1:-89983496 None:intergenic
GTTCTTTGAATTATGCAATG+AGG 0.563916 6_1:+89983686 Msa0897630:three_prime_UTR
GATGAGAATAATTTGGACAG+TGG 0.564663 6_1:-89980279 None:intergenic
ATATGTGCAAGAATTCTTGA+GGG 0.565567 6_1:-89983495 None:intergenic
GGATTCCTTTGAGATATGGA+TGG 0.566300 6_1:+89983609 Msa0897630:three_prime_UTR
GAGTCATAGATTGGTGAGTG+TGG 0.597085 6_1:+89983265 Msa0897630:CDS
CAAAGAAAGTGCCAGTGTTG+CGG 0.604498 6_1:-89981993 None:intergenic
TTCAATGGTACTGGAACTGT+AGG 0.605405 6_1:-89983353 None:intergenic
AGCAACAGGTGTTGAATGGA+AGG 0.610713 6_1:-89980613 None:intergenic
CAATTCCATCCATATCTCAA+AGG 0.611390 6_1:-89983614 None:intergenic
CTTGCACCACTTCCATACAA+AGG 0.614462 6_1:-89983308 None:intergenic
TAGTATAGTGGACGACGTGG+TGG 0.617154 6_1:-89980546 None:intergenic
TGTGGCAGTGATGGGTAGCA+AGG 0.618679 6_1:+89983283 Msa0897630:CDS
GATACAGCTCAGAACTCAGA+AGG 0.623173 6_1:+89983642 Msa0897630:three_prime_UTR
AGCCGGTTCGGATTCGACTG+GGG 0.626576 6_1:+89981924 Msa0897630:CDS
TGAGGACAAAGATCATGTGT+TGG 0.634779 6_1:+89983704 Msa0897630:three_prime_UTR
AGAAGGCAAGATGATTAGGA+CGG 0.636424 6_1:+89983659 Msa0897630:three_prime_UTR
TGCAAGCCTTAGCAGCACAA+CGG 0.643607 6_1:+89983325 Msa0897630:CDS
CGGTTCGGATTCGACTGGGG+TGG 0.649583 6_1:+89981927 Msa0897630:CDS
TGAATGGAAGGATGTAGTGA+CGG 0.653571 6_1:-89980601 None:intergenic
GGAATGGTGGTTATTCTAGG+AGG 0.655134 6_1:+89980763 Msa0897630:CDS
AGATATGGATGGAATTGATG+GGG 0.666943 6_1:+89983620 Msa0897630:three_prime_UTR
GTTCATGGTGATCATATCAG+TGG 0.667894 6_1:-89981950 None:intergenic
GTGGAAGAAGAAGAATGATG+AGG 0.698905 6_1:-89980639 None:intergenic
ACGTAGTATAGTGGACGACG+TGG 0.744646 6_1:-89980549 None:intergenic
GTTCAAAGTCCATCACACGA+CGG 0.801345 6_1:+89980570 Msa0897630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTATTTTTTTTATTAA+TGG + chr6_1:89983588-89983607 Msa0897630:three_prime_UTR 0.0%
!!! ATGATAAATTTTTCATTTTT+AGG + chr6_1:89981074-89981093 Msa0897630:intron 10.0%
!!! ATATTCAATTTTTCATTTTA+AGG + chr6_1:89981500-89981519 Msa0897630:intron 10.0%
!! TTAATTAATGTAATCAAAAT+GGG - chr6_1:89983813-89983832 None:intergenic 10.0%
!! ATTAATTAATGTAATCAAAA+TGG - chr6_1:89983814-89983833 None:intergenic 10.0%
!!! TTTTGATTATTGTTTTTTGA+GGG + chr6_1:89980898-89980917 Msa0897630:intron 15.0%
!!! TTTATTGATTTTGAGATTTT+AGG + chr6_1:89980989-89981008 Msa0897630:intron 15.0%
!!! TGATAAATTTTGCTTTTTTT+AGG + chr6_1:89981032-89981051 Msa0897630:intron 15.0%
!!! ATGTTCAATTTTTCATTTTA+GGG + chr6_1:89981116-89981135 Msa0897630:intron 15.0%
!!! TTTTAAGATGAAATTTTAAC+TGG + chr6_1:89981174-89981193 Msa0897630:intron 15.0%
!!! TTTAAGATGAAATTTTAACT+GGG + chr6_1:89981175-89981194 Msa0897630:intron 15.0%
!!! AAGTTCAATTTTTCATTTTT+AGG + chr6_1:89981325-89981344 Msa0897630:intron 15.0%
!!! AGTTCAATTTTTCATTTTTA+GGG + chr6_1:89981326-89981345 Msa0897630:intron 15.0%
!! TTAATACTTATTTGCATTTA+TGG + chr6_1:89982306-89982325 Msa0897630:intron 15.0%
!! TAATTAAGTTTACTTTCTAA+TGG - chr6_1:89982631-89982650 None:intergenic 15.0%
!! ACTTTCTCTATAAATTATTA+AGG - chr6_1:89982678-89982697 None:intergenic 15.0%
!! AAGATTAAATGATTTATGTT+TGG + chr6_1:89982725-89982744 Msa0897630:intron 15.0%
!! TGAATATTCATATATGTTAA+AGG + chr6_1:89983521-89983540 Msa0897630:exon 15.0%
!! GAATATTCATATATGTTAAA+GGG + chr6_1:89983522-89983541 Msa0897630:exon 15.0%
!! AGAAATAGAAGAAAAAACAT+AGG + chr6_1:89980403-89980422 Msa0897630:five_prime_UTR 20.0%
!!! TTTGTTTTCGATTATTCTTT+GGG + chr6_1:89980830-89980849 Msa0897630:CDS 20.0%
!!! GTTTTGATTATTGTTTTTTG+AGG + chr6_1:89980897-89980916 Msa0897630:intron 20.0%
!!! CATGTTCAATTTTTCATTTT+AGG + chr6_1:89981115-89981134 Msa0897630:intron 20.0%
!!! TTTTAAGTTGAAATGTAAAC+TGG + chr6_1:89981257-89981276 Msa0897630:intron 20.0%
!!! TTTAAGTTGAAATGTAAACT+GGG + chr6_1:89981258-89981277 Msa0897630:intron 20.0%
!! TCAACCTAAAAATGAAAAAT+TGG - chr6_1:89981081-89981100 None:intergenic 20.0%
!!! TATCCATTTTCCATTTTTTT+AGG + chr6_1:89981780-89981799 Msa0897630:intron 20.0%
!! AAGATATCATCCTAAAAAAA+TGG - chr6_1:89981793-89981812 None:intergenic 20.0%
!!! GTAGTTATGAAAATTGATTT+TGG + chr6_1:89982112-89982131 Msa0897630:intron 20.0%
!! ATTTAAATCTGAATTCTGTT+TGG + chr6_1:89982546-89982565 Msa0897630:intron 20.0%
!! TAAACTTAATTACGTTTGAA+AGG + chr6_1:89982639-89982658 Msa0897630:intron 20.0%
!! TGTGTTAGAAAAATACAAAA+GGG - chr6_1:89983206-89983225 None:intergenic 20.0%
!! ATGTGTTAGAAAAATACAAA+AGG - chr6_1:89983207-89983226 None:intergenic 20.0%
!!! GAAAGTGTAAAAGAATTATT+AGG + chr6_1:89983558-89983577 Msa0897630:three_prime_UTR 20.0%
! AGAGAAAGATGAGAATAATT+TGG - chr6_1:89980289-89980308 None:intergenic 25.0%
!!! TTTGTAGTTTGTGTTAGTTT+TGG - chr6_1:89980330-89980349 None:intergenic 25.0%
! ATTGATGTTATTGAAGAAGA+AGG + chr6_1:89980723-89980742 Msa0897630:CDS 25.0%
! TTGATGTTATTGAAGAAGAA+GGG + chr6_1:89980724-89980743 Msa0897630:CDS 25.0%
!!! CTTTGTTTTCGATTATTCTT+TGG + chr6_1:89980829-89980848 Msa0897630:CDS 25.0%
!! TTGTTTTCGATTATTCTTTG+GGG + chr6_1:89980831-89980850 Msa0897630:CDS 25.0%
!!! TATTGTTTTTTGAGGGTTTT+TGG + chr6_1:89980905-89980924 Msa0897630:intron 25.0%
!! TTTGATTGATTGTTCAGTTT+TGG + chr6_1:89980946-89980965 Msa0897630:intron 25.0%
!!! TTTTGGAATGTGATTTGAAT+TGG + chr6_1:89980963-89980982 Msa0897630:intron 25.0%
!! TTTGGAATGTGATTTGAATT+GGG + chr6_1:89980964-89980983 Msa0897630:intron 25.0%
!!! TTTTAGGATGTGATTTGAAT+TGG + chr6_1:89981005-89981024 Msa0897630:intron 25.0%
!! TTTAGGATGTGATTTGAATT+GGG + chr6_1:89981006-89981025 Msa0897630:intron 25.0%
!!! TTTTAGGATGAAATTTGAAC+TGG + chr6_1:89981048-89981067 Msa0897630:intron 25.0%
!! TTTAGGATGAAATTTGAACT+GGG + chr6_1:89981049-89981068 Msa0897630:intron 25.0%
!!! TTTTAGGTTGAAATGTAAAC+TGG + chr6_1:89981090-89981109 Msa0897630:intron 25.0%
!!! TTTAGGTTGAAATGTAAACT+GGG + chr6_1:89981091-89981110 Msa0897630:intron 25.0%
!! ATGATCAGTTTTGCATTTTT+AGG + chr6_1:89981200-89981219 Msa0897630:intron 25.0%
!!! TTTTAAGTTGAAATCTGAAC+TGG + chr6_1:89981299-89981318 Msa0897630:intron 25.0%
!!! TTTAAGTTGAAATCTGAACT+GGG + chr6_1:89981300-89981319 Msa0897630:intron 25.0%
! TTGAAAACTTGAAATCTGAA+TGG + chr6_1:89981349-89981368 Msa0897630:intron 25.0%
! TGAAAACTTGAAATCTGAAT+GGG + chr6_1:89981350-89981369 Msa0897630:intron 25.0%
!! TTTAAAGTTGGAATTTGAAC+TGG + chr6_1:89981393-89981412 Msa0897630:intron 25.0%
! TTAAAGTTGGAATTTGAACT+GGG + chr6_1:89981394-89981413 Msa0897630:intron 25.0%
!!! TGCTCCAATTTTTCATTTTT+AGG + chr6_1:89981690-89981709 Msa0897630:intron 25.0%
! AATGAAAGAAGATAAGAACT+TGG + chr6_1:89981753-89981772 Msa0897630:intron 25.0%
! CATCCTAAAAAAATGGAAAA+TGG - chr6_1:89981786-89981805 None:intergenic 25.0%
!!! TTTTAGGATGATATCTTAAG+TGG + chr6_1:89981796-89981815 Msa0897630:intron 25.0%
!! TTTAGGATGATATCTTAAGT+GGG + chr6_1:89981797-89981816 Msa0897630:intron 25.0%
!!! TGTTCAATTTTTGCTTTTCA+AGG + chr6_1:89981823-89981842 Msa0897630:intron 25.0%
! TACAAGTTAGAATTCATTCT+AGG + chr6_1:89982256-89982275 Msa0897630:intron 25.0%
! ACAAGTTAGAATTCATTCTA+GGG + chr6_1:89982257-89982276 Msa0897630:intron 25.0%
! ATTAAGAAGCTTCAATTTGT+AGG - chr6_1:89982362-89982381 None:intergenic 25.0%
!!! TATCAAAATCATTTTTCGCA+CGG - chr6_1:89982494-89982513 None:intergenic 25.0%
! AATCCATTCATGCATATATA+TGG + chr6_1:89983143-89983162 Msa0897630:intron 25.0%
! TGAAGCACAAATACTATAAA+AGG + chr6_1:89983423-89983442 Msa0897630:CDS 25.0%
ATCAACATAGTGTTAATCCA+TGG + chr6_1:89980694-89980713 Msa0897630:CDS 30.0%
ATAACATCAATATGCTTCCA+TGG - chr6_1:89980714-89980733 None:intergenic 30.0%
! TATTGAAGAAGAAGGGTTTT+TGG + chr6_1:89980731-89980750 Msa0897630:CDS 30.0%
!!! TATTTTCTGGCTTTTGTTGT+TGG + chr6_1:89980789-89980808 Msa0897630:CDS 30.0%
!!! ATTTTCTGGCTTTTGTTGTT+GGG + chr6_1:89980790-89980809 Msa0897630:CDS 30.0%
! TTTTTGGATGAAATTGGAAC+TGG + chr6_1:89980921-89980940 Msa0897630:intron 30.0%
! TTTTGGATGAAATTGGAACT+GGG + chr6_1:89980922-89980941 Msa0897630:intron 30.0%
! TTTAGGGTTGAAATATGAAC+TGG + chr6_1:89981132-89981151 Msa0897630:intron 30.0%
TTAGGGTTGAAATATGAACT+GGG + chr6_1:89981133-89981152 Msa0897630:intron 30.0%
!!! TTTTAGGATGATGTTTGAAC+TGG + chr6_1:89981216-89981235 Msa0897630:intron 30.0%
! TTTAGGATGATGTTTGAACT+GGG + chr6_1:89981217-89981236 Msa0897630:intron 30.0%
!! CCAACTTTAAAGTGAAACAT+TGG - chr6_1:89981384-89981403 None:intergenic 30.0%
!! CCAATGTTTCACTTTAAAGT+TGG + chr6_1:89981381-89981400 Msa0897630:intron 30.0%
TGCAGAATGAAATTTGAACT+GGG + chr6_1:89981435-89981454 Msa0897630:intron 30.0%
GTCATGATCAAATTTGGATT+AGG + chr6_1:89981457-89981476 Msa0897630:intron 30.0%
TGTATTTCTCATGAACAACT+TGG + chr6_1:89981637-89981656 Msa0897630:intron 30.0%
ATGAACAACTTGGAATGTTA+GGG + chr6_1:89981647-89981666 Msa0897630:intron 30.0%
GAATTTCAAAGTAGAGTTCA+TGG - chr6_1:89981968-89981987 None:intergenic 30.0%
AGCAATGACTATATCTGAAT+AGG - chr6_1:89982052-89982071 None:intergenic 30.0%
! GTAGCTGAGATTTATAGTTT+TGG - chr6_1:89982239-89982258 None:intergenic 30.0%
CAAGTTAGAATTCATTCTAG+GGG + chr6_1:89982258-89982277 Msa0897630:intron 30.0%
AGTTATCCTCATTTACTACA+TGG - chr6_1:89982573-89982592 None:intergenic 30.0%
!! TATGTTCTGAAGTCTATACA+TGG - chr6_1:89982596-89982615 None:intergenic 30.0%
TTGAAGATGAAGTTTGAAGA+TGG - chr6_1:89982809-89982828 None:intergenic 30.0%
!! ATGTTTCGATTCACTTTTGA+GGG - chr6_1:89982924-89982943 None:intergenic 30.0%
!! TATGTTTCGATTCACTTTTG+AGG - chr6_1:89982925-89982944 None:intergenic 30.0%
!! ATGAATGGATTCACTTTTGT+GGG - chr6_1:89983134-89983153 None:intergenic 30.0%
CAACCATATATATGCATGAA+TGG - chr6_1:89983149-89983168 None:intergenic 30.0%
! ACATTGATTATTGCAGATGA+AGG + chr6_1:89983223-89983242 Msa0897630:intron 30.0%
ATCACAAAGTTAAGGTTCAA+TGG - chr6_1:89983371-89983390 None:intergenic 30.0%
TTGATCTCATCACAAAGTTA+AGG - chr6_1:89983379-89983398 None:intergenic 30.0%
AGCTTATGTACTTGGAAATT+TGG + chr6_1:89983400-89983419 Msa0897630:CDS 30.0%
ATATGTGCAAGAATTCTTGA+GGG - chr6_1:89983498-89983517 None:intergenic 30.0%
!! TAATGGATTCCTTTGAGATA+TGG + chr6_1:89983605-89983624 Msa0897630:three_prime_UTR 30.0%
GTTCTTTGAATTATGCAATG+AGG + chr6_1:89983686-89983705 Msa0897630:three_prime_UTR 30.0%
GATGAGAATAATTTGGACAG+TGG - chr6_1:89980282-89980301 None:intergenic 35.0%
CTTAGAAGCAGAAAAATGAG+TGG - chr6_1:89980661-89980680 None:intergenic 35.0%
!! TTTTTGGAGAAAGAGAGGAA+TGG + chr6_1:89980747-89980766 Msa0897630:CDS 35.0%
!!! TTCTAGGAGGTGTTATTTTC+TGG + chr6_1:89980776-89980795 Msa0897630:CDS 35.0%
CAAACCTTGATCACAATCTT+GGG - chr6_1:89980876-89980895 None:intergenic 35.0%
ACAAACCTTGATCACAATCT+TGG - chr6_1:89980877-89980896 None:intergenic 35.0%
!! TGAGGGTTTTTGGATGAAAT+TGG + chr6_1:89980915-89980934 Msa0897630:intron 35.0%
CTGCAGAATGAAATTTGAAC+TGG + chr6_1:89981434-89981453 Msa0897630:intron 35.0%
AACTGGGTCATGATCAAATT+TGG + chr6_1:89981451-89981470 Msa0897630:intron 35.0%
CATGAACAACTTGGAATGTT+AGG + chr6_1:89981646-89981665 Msa0897630:intron 35.0%
!!! TTTTAGGTTGAAACCTGAAC+TGG + chr6_1:89981706-89981725 Msa0897630:intron 35.0%
AATTGATCAAGAACCAGTTC+AGG - chr6_1:89981722-89981741 None:intergenic 35.0%
TCAGATATAGTCATTGCTTC+AGG + chr6_1:89982054-89982073 Msa0897630:intron 35.0%
GATTCACTAACTCGTTTGTT+TGG + chr6_1:89982085-89982104 Msa0897630:intron 35.0%
! ACTTCAGCGTATGTTTGTTT+TGG + chr6_1:89982443-89982462 Msa0897630:intron 35.0%
TTTGGACCATGTAGTAAATG+AGG + chr6_1:89982564-89982583 Msa0897630:intron 35.0%
AGAGAAAGTTGAGTTACAAG+TGG + chr6_1:89982689-89982708 Msa0897630:intron 35.0%
ATTCTCCCCAATGTCAATTT+GGG - chr6_1:89982979-89982998 None:intergenic 35.0%
ATGTTTCGATTAACCCACTT+GGG - chr6_1:89983063-89983082 None:intergenic 35.0%
TATGTTTCGATTAACCCACT+TGG - chr6_1:89983064-89983083 None:intergenic 35.0%
!! TGAATGGATTCACTTTTGTG+GGG - chr6_1:89983133-89983152 None:intergenic 35.0%
!! CATGAATGGATTCACTTTTG+TGG - chr6_1:89983135-89983154 None:intergenic 35.0%
TGAAGGAGTTTCATCAATCT+AGG + chr6_1:89983240-89983259 Msa0897630:CDS 35.0%
ATCTAGGAAGAGTCATAGAT+TGG + chr6_1:89983256-89983275 Msa0897630:CDS 35.0%
AAGTTAAGGTTCAATGGTAC+TGG - chr6_1:89983365-89983384 None:intergenic 35.0%
AGATCAAGAGCTTATGTACT+TGG + chr6_1:89983392-89983411 Msa0897630:CDS 35.0%
CATATGTGCAAGAATTCTTG+AGG - chr6_1:89983499-89983518 None:intergenic 35.0%
CAATTCCATCCATATCTCAA+AGG - chr6_1:89983617-89983636 None:intergenic 35.0%
TGAGATATGGATGGAATTGA+TGG + chr6_1:89983618-89983637 Msa0897630:three_prime_UTR 35.0%
GAGATATGGATGGAATTGAT+GGG + chr6_1:89983619-89983638 Msa0897630:three_prime_UTR 35.0%
AGATATGGATGGAATTGATG+GGG + chr6_1:89983620-89983639 Msa0897630:three_prime_UTR 35.0%
!! ACAAAGATCATGTGTTGGTT+CGG + chr6_1:89983709-89983728 Msa0897630:three_prime_UTR 35.0%
AGTGGAAGAATCTGTACAAC+TGG - chr6_1:89980264-89980283 None:intergenic 40.0%
GGACTTTGAACGTAGTATAG+TGG - chr6_1:89980561-89980580 None:intergenic 40.0%
TGAATGGAAGGATGTAGTGA+CGG - chr6_1:89980604-89980623 None:intergenic 40.0%
GTGGAAGAAGAAGAATGATG+AGG - chr6_1:89980642-89980661 None:intergenic 40.0%
!! AAGGGTTTTTGGAGAAAGAG+AGG + chr6_1:89980742-89980761 Msa0897630:CDS 40.0%
AGAGGAATGGTGGTTATTCT+AGG + chr6_1:89980760-89980779 Msa0897630:CDS 40.0%
ATCACAATCTTGGGCTTCAT+AGG - chr6_1:89980867-89980886 None:intergenic 40.0%
ACAACTTGGAATGTTAGGGT+TGG + chr6_1:89981651-89981670 Msa0897630:intron 40.0%
TGTTAGGGTTGGAATCTGAT+TGG + chr6_1:89981662-89981681 Msa0897630:intron 40.0%
GTTAGGGTTGGAATCTGATT+GGG + chr6_1:89981663-89981682 Msa0897630:intron 40.0%
GATCATGTTACTGTTCAAGC+CGG + chr6_1:89981907-89981926 Msa0897630:CDS 40.0%
GTTCATGGTGATCATATCAG+TGG - chr6_1:89981953-89981972 None:intergenic 40.0%
AAATTCACATACCGCAACAC+TGG + chr6_1:89981982-89982001 Msa0897630:CDS 40.0%
TCTGAATAGGACAGTTCCAA+GGG - chr6_1:89982039-89982058 None:intergenic 40.0%
ATCTGAATAGGACAGTTCCA+AGG - chr6_1:89982040-89982059 None:intergenic 40.0%
TAGTCCTTTGTGACTGTGTT+TGG + chr6_1:89982331-89982350 Msa0897630:intron 40.0%
! TTTTCACAGTGAGTTTGCGT+TGG + chr6_1:89982381-89982400 Msa0897630:intron 40.0%
TTTCACAGTGAGTTTGCGTT+GGG + chr6_1:89982382-89982401 Msa0897630:intron 40.0%
CATACGCTGAAGTTTGTGTT+TGG - chr6_1:89982436-89982455 None:intergenic 40.0%
!!! CGTATGTTTGTTTTGGCGTT+GGG + chr6_1:89982450-89982469 Msa0897630:intron 40.0%
GATTCTCCCCAATGTCAATT+TGG - chr6_1:89982980-89982999 None:intergenic 40.0%
TTGCACCACTTCCATACAAA+GGG - chr6_1:89983310-89983329 None:intergenic 40.0%
! TTCAATGGTACTGGAACTGT+AGG - chr6_1:89983356-89983375 None:intergenic 40.0%
GATTCAATGTTCCATCACCT+TGG + chr6_1:89983445-89983464 Msa0897630:CDS 40.0%
TTTCTTAGGATCCAAGGTGA+TGG - chr6_1:89983459-89983478 None:intergenic 40.0%
ACCTTGGATCCTAAGAAACT+TGG + chr6_1:89983461-89983480 Msa0897630:CDS 40.0%
ACCAAGTTTCTTAGGATCCA+AGG - chr6_1:89983465-89983484 None:intergenic 40.0%
! ACTAGAGCACCAAGTTTCTT+AGG - chr6_1:89983473-89983492 None:intergenic 40.0%
!! GGATTCCTTTGAGATATGGA+TGG + chr6_1:89983609-89983628 Msa0897630:three_prime_UTR 40.0%
ACTCAGAAGGCAAGATGATT+AGG + chr6_1:89983655-89983674 Msa0897630:three_prime_UTR 40.0%
AGAAGGCAAGATGATTAGGA+CGG + chr6_1:89983659-89983678 Msa0897630:three_prime_UTR 40.0%
TGAGGACAAAGATCATGTGT+TGG + chr6_1:89983704-89983723 Msa0897630:three_prime_UTR 40.0%
GTTCAAAGTCCATCACACGA+CGG + chr6_1:89980570-89980589 Msa0897630:CDS 45.0%
!!! GTTTTTTCACCGTCGTGTGA+TGG - chr6_1:89980582-89980601 None:intergenic 45.0%
!! AGCAACAGGTGTTGAATGGA+AGG - chr6_1:89980616-89980635 None:intergenic 45.0%
!! GTGAAGCAACAGGTGTTGAA+TGG - chr6_1:89980620-89980639 None:intergenic 45.0%
GAATGATGAGGTGAAGCAAC+AGG - chr6_1:89980630-89980649 None:intergenic 45.0%
TTGGAGAAAGAGAGGAATGG+TGG + chr6_1:89980750-89980769 Msa0897630:CDS 45.0%
GGAATGGTGGTTATTCTAGG+AGG + chr6_1:89980763-89980782 Msa0897630:CDS 45.0%
GAAGCCCAAGATTGTGATCA+AGG + chr6_1:89980869-89980888 Msa0897630:CDS 45.0%
TGTTACTGTTCAAGCCGGTT+CGG + chr6_1:89981912-89981931 Msa0897630:CDS 45.0%
!! CAAAGAAAGTGCCAGTGTTG+CGG - chr6_1:89981996-89982015 None:intergenic 45.0%
ACAGCCAAACACAGTCACAA+AGG - chr6_1:89982338-89982357 None:intergenic 45.0%
! GCTGAAGTTTGTGTTTGGTG+TGG - chr6_1:89982431-89982450 None:intergenic 45.0%
!!! GCGTATGTTTGTTTTGGCGT+TGG + chr6_1:89982449-89982468 Msa0897630:intron 45.0%
TAGCCCACCCAAATTGACAT+TGG + chr6_1:89982969-89982988 Msa0897630:intron 45.0%
AGCCCACCCAAATTGACATT+GGG + chr6_1:89982970-89982989 Msa0897630:intron 45.0%
TCCCCAATGTCAATTTGGGT+GGG - chr6_1:89982975-89982994 None:intergenic 45.0%
TTCGATTAACCCACTTGGGT+TGG - chr6_1:89983059-89983078 None:intergenic 45.0%
GAGTCATAGATTGGTGAGTG+TGG + chr6_1:89983265-89983284 Msa0897630:CDS 45.0%
AAGGTCCCTTTGTATGGAAG+TGG + chr6_1:89983302-89983321 Msa0897630:CDS 45.0%
CTTGCACCACTTCCATACAA+AGG - chr6_1:89983311-89983330 None:intergenic 45.0%
GATACAGCTCAGAACTCAGA+AGG + chr6_1:89983642-89983661 Msa0897630:three_prime_UTR 45.0%
!! TAGTATAGTGGACGACGTGG+TGG - chr6_1:89980549-89980568 None:intergenic 50.0%
!! ACGTAGTATAGTGGACGACG+TGG - chr6_1:89980552-89980571 None:intergenic 50.0%
CGCAACACTGGCACTTTCTT+TGG + chr6_1:89981994-89982013 Msa0897630:CDS 50.0%
! CCAAGGGTGTAGCTGAAACA+TGG - chr6_1:89982023-89982042 None:intergenic 50.0%
CCATGTTTCAGCTACACCCT+TGG + chr6_1:89982020-89982039 Msa0897630:intron 50.0%
GCCCACCCAAATTGACATTG+GGG + chr6_1:89982971-89982990 Msa0897630:intron 50.0%
CTCCCCAATGTCAATTTGGG+TGG - chr6_1:89982976-89982995 None:intergenic 50.0%
GAATCGAACTTGAGACCTCG+AGG + chr6_1:89982995-89983014 Msa0897630:intron 50.0%
ATTGGTGAGTGTGGCAGTGA+TGG + chr6_1:89983274-89983293 Msa0897630:CDS 50.0%
TTGGTGAGTGTGGCAGTGAT+GGG + chr6_1:89983275-89983294 Msa0897630:CDS 50.0%
GGTAGCAAGGTCCCTTTGTA+TGG + chr6_1:89983296-89983315 Msa0897630:CDS 50.0%
TGCAAGCCTTAGCAGCACAA+CGG + chr6_1:89983325-89983344 Msa0897630:CDS 50.0%
! CGACGTGGTGGTGATCTTGT+TGG - chr6_1:89980537-89980556 None:intergenic 55.0%
!! AAGCCGGTTCGGATTCGACT+GGG + chr6_1:89981923-89981942 Msa0897630:CDS 55.0%
TCGAACTTGAGACCTCGAGG+AGG + chr6_1:89982998-89983017 Msa0897630:intron 55.0%
!! TTGGGTTGGCCTGGTGGTAT+TGG - chr6_1:89983045-89983064 None:intergenic 55.0%
!! TTAACCCACTTGGGTTGGCC+TGG - chr6_1:89983054-89983073 None:intergenic 55.0%
!! TGTGGCAGTGATGGGTAGCA+AGG + chr6_1:89983283-89983302 Msa0897630:CDS 55.0%
GCAAGCCTTAGCAGCACAAC+GGG + chr6_1:89983326-89983345 Msa0897630:CDS 55.0%
! GCATACCCGTTGTGCTGCTA+AGG - chr6_1:89983334-89983353 None:intergenic 55.0%
!! CAAGCCGGTTCGGATTCGAC+TGG + chr6_1:89981922-89981941 Msa0897630:CDS 60.0%
!! AGCCGGTTCGGATTCGACTG+GGG + chr6_1:89981924-89981943 Msa0897630:CDS 60.0%
CACCCCAGTCGAATCCGAAC+CGG - chr6_1:89981929-89981948 None:intergenic 60.0%
TGAGAGTCTGCTCCTCCTCG+AGG - chr6_1:89983013-89983032 None:intergenic 60.0%
TCGAGGAGGAGCAGACTCTC+AGG + chr6_1:89983012-89983031 Msa0897630:intron 60.0%
GGCCTCAAGCCAATACCACC+AGG + chr6_1:89983033-89983052 Msa0897630:intron 60.0%
!! GGCCTGGTGGTATTGGCTTG+AGG - chr6_1:89983038-89983057 None:intergenic 60.0%
TACCACCAGGCCAACCCAAG+TGG + chr6_1:89983046-89983065 Msa0897630:intron 60.0%
ACCACCAGGCCAACCCAAGT+GGG + chr6_1:89983047-89983066 Msa0897630:intron 60.0%
! CGGTTCGGATTCGACTGGGG+TGG + chr6_1:89981927-89981946 Msa0897630:CDS 65.0%
!! ACCCACTTGGGTTGGCCTGG+TGG - chr6_1:89983051-89983070 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 89980245 89983843 89980245 ID=Msa0897630;Name=Msa0897630
chr6_1 mRNA 89980245 89983843 89980245 ID=Msa0897630-mRNA-1;Parent=Msa0897630;Name=Msa0897630-mRNA-1;_AED=0.03;_eAED=0.03;_QI=232|0.5|0.66|1|1|1|3|320|275
chr6_1 exon 89980245 89980890 89980245 ID=Msa0897630-mRNA-1:exon:15251;Parent=Msa0897630-mRNA-1
chr6_1 exon 89981895 89982023 89981895 ID=Msa0897630-mRNA-1:exon:15252;Parent=Msa0897630-mRNA-1
chr6_1 exon 89983239 89983843 89983239 ID=Msa0897630-mRNA-1:exon:15253;Parent=Msa0897630-mRNA-1
chr6_1 five_prime_UTR 89980245 89980476 89980245 ID=Msa0897630-mRNA-1:five_prime_utr;Parent=Msa0897630-mRNA-1
chr6_1 CDS 89980477 89980890 89980477 ID=Msa0897630-mRNA-1:cds;Parent=Msa0897630-mRNA-1
chr6_1 CDS 89981895 89982023 89981895 ID=Msa0897630-mRNA-1:cds;Parent=Msa0897630-mRNA-1
chr6_1 CDS 89983239 89983523 89983239 ID=Msa0897630-mRNA-1:cds;Parent=Msa0897630-mRNA-1
chr6_1 three_prime_UTR 89983524 89983843 89983524 ID=Msa0897630-mRNA-1:three_prime_utr;Parent=Msa0897630-mRNA-1
Gene Sequence

>Msa0897630

ATGCATACAAAAACAGATTCAGAAGTTACAACAACAAGTGTAGCTACATCAACATCACCAACAAGATCACCACCACGTCGTCCACTATACTACGTTCAAAGTCCATCACACGACGGTGAAAAAACCGTCACTACATCCTTCCATTCAACACCTGTTGCTTCACCTCATCATTCTTCTTCTTCCACTCATTTTTCTGCTTCTAAGAAAGAACAACAACATCAACATAGTGTTAATCCATGGAAGCATATTGATGTTATTGAAGAAGAAGGGTTTTTGGAGAAAGAGAGGAATGGTGGTTATTCTAGGAGGTGTTATTTTCTGGCTTTTGTTGTTGGGTTCTTTGTTTTGTTCTCTTTGTTTTCGATTATTCTTTGGGGTGCTAGTAGACCTATGAAGCCCAAGATTGTGATCAAGAGCATAAAGTTTGATCATGTTACTGTTCAAGCCGGTTCGGATTCGACTGGGGTGGCCACTGATATGATCACCATGAACTCTACTTTGAAATTCACATACCGCAACACTGGCACTTTCTTTGGAGTCCATATGAAGGAGTTTCATCAATCTAGGAAGAGTCATAGATTGGTGAGTGTGGCAGTGATGGGTAGCAAGGTCCCTTTGTATGGAAGTGGTGCAAGCCTTAGCAGCACAACGGGTATGCCTACAGTTCCAGTACCATTGAACCTTAACTTTGTGATGAGATCAAGAGCTTATGTACTTGGAAATTTGGTGAAGCACAAATACTATAAAAGGATTCAATGTTCCATCACCTTGGATCCTAAGAAACTTGGTGCTCTAGTTTCCCTCAAGAATTCTTGCACATATGATTGA
Protein sequence

>Msa0897630

MHTKTDSEVTTTSVATSTSPTRSPPRRPLYYVQSPSHDGEKTVTTSFHSTPVASPHHSSSSTHFSASKKEQQHQHSVNPWKHIDVIEEEGFLEKERNGGYSRRCYFLAFVVGFFVLFSLFSIILWGASRPMKPKIVIKSIKFDHVTVQAGSDSTGVATDMITMNSTLKFTYRNTGTFFGVHMKEFHQSRKSHRLVSVAVMGSKVPLYGSGASLSSTTGMPTVPVPLNLNFVMRSRAYVLGNLVKHKYYKRIQCSITLDPKKLGALVSLKNSCTYD*