Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0919970 | XP_024631910.1 | 88.764 | 89 | 10 | 0 | 3 | 91 | 1032 | 1120 | 3.01e-44 | 163 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042770 | Msa0919970 | 0.915225 | 7.580818e-85 | -8.615850e-47 |
| Msa0622630 | Msa0919970 | 0.864587 | 1.092110e-64 | -8.615850e-47 |
| Msa0622660 | Msa0919970 | 0.818584 | 1.815863e-52 | -8.615850e-47 |
| Msa0623820 | Msa0919970 | 0.826101 | 3.263575e-54 | -8.615850e-47 |
| Msa0727130 | Msa0919970 | 0.822803 | 1.948004e-53 | -8.615850e-47 |
| Msa0773610 | Msa0919970 | 0.801533 | 8.610242e-49 | -8.615850e-47 |
| Msa0339790 | Msa0919970 | 0.823773 | 1.156595e-53 | -8.615850e-47 |
| Msa0919970 | Msa1070950 | 0.811499 | 6.792076e-51 | -8.615850e-47 |
| Msa0919970 | Msa1187290 | 0.881872 | 1.673042e-70 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTCGTGCGGTTGTTTAA+AGG | 0.271890 | 6_2:+53391170 | Msa0919970:three_prime_UTR |
| GGTGCTTAAAATTTATTATC+TGG | 0.297194 | 6_2:+53391140 | Msa0919970:three_prime_UTR |
| GGAAGCATACGGTCCTTTCT+TGG | 0.323111 | 6_2:+53390817 | Msa0919970:CDS |
| GGAAGTCTTGTTGATTTAGT+TGG | 0.335009 | 6_2:+53390785 | Msa0919970:CDS |
| GTTTCGTGCGGTTGTTTAAA+GGG | 0.347112 | 6_2:+53391171 | Msa0919970:three_prime_UTR |
| TTGAATTGTGCTTCTCGCCT+TGG | 0.385538 | 6_2:-53390669 | None:intergenic |
| TGTGTGAAGTATACTTGTTT+TGG | 0.393011 | 6_2:-53390605 | None:intergenic |
| TGGTATGGAACTTCTTTCTC+CGG | 0.445848 | 6_2:-53390710 | None:intergenic |
| AACAAATCCAAAGGATTTCA+AGG | 0.450703 | 6_2:+53391101 | Msa0919970:three_prime_UTR |
| ATTGCAAATGACAATCTTGA+AGG | 0.456999 | 6_2:+53390764 | Msa0919970:CDS |
| AGAGGTAGAGGCAGCGGAAG+AGG | 0.457632 | 6_2:+53391036 | Msa0919970:CDS |
| ATTGTGTGATTGATTCATCT+TGG | 0.474013 | 6_2:-53390730 | None:intergenic |
| GTGCGGTTGTTTAAAGGGCG+TGG | 0.502973 | 6_2:+53391176 | Msa0919970:three_prime_UTR |
| ATACGGTCCTTTCTTGGATG+AGG | 0.503815 | 6_2:+53390823 | Msa0919970:CDS |
| GTGATTGATTCATCTTGGTA+TGG | 0.505565 | 6_2:-53390725 | None:intergenic |
| AATTTATTATCTGGTAGCAT+AGG | 0.515942 | 6_2:+53391149 | Msa0919970:three_prime_UTR |
| TGATTTAGTTGGAGAAGTTG+AGG | 0.526516 | 6_2:+53390796 | Msa0919970:CDS |
| CGAATGTTAGGTAGAGGTAG+AGG | 0.532296 | 6_2:+53391024 | Msa0919970:intron |
| TTAGGTAGAGGTAGAGGCAG+CGG | 0.532621 | 6_2:+53391030 | Msa0919970:intron |
| AAGGTAACCTCATCCAAGAA+AGG | 0.563434 | 6_2:-53390830 | None:intergenic |
| ATCTTCTCAAACAAATCCAA+AGG | 0.593214 | 6_2:+53391092 | Msa0919970:three_prime_UTR |
| GTTGGCTGTGATTAAGACCA+AGG | 0.613774 | 6_2:+53390652 | Msa0919970:CDS |
| TACTTCACACAGTATCCTGA+AGG | 0.615568 | 6_2:+53390617 | Msa0919970:CDS |
| GGAGAAGTTGAGGAAGCATA+CGG | 0.617796 | 6_2:+53390806 | Msa0919970:CDS |
| CTGGTAGCATAGGTTTCGTG+CGG | 0.636336 | 6_2:+53391159 | Msa0919970:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAAGGTATTTTTATGATATA+AGG | + | chr6_2:53391119-53391138 | Msa0919970:three_prime_UTR | 20.0% |
| !!! | AATTTTAGAAGATAGTAAGC+CGG | + | chr6_2:53390691-53390710 | Msa0919970:CDS | 25.0% |
| ! | AATATCTTTATCCCATTCAA+AGG | - | chr6_2:53390852-53390871 | None:intergenic | 25.0% |
| ! | AAAAATACCTTGAAATCCTT+TGG | - | chr6_2:53391111-53391130 | None:intergenic | 25.0% |
| ! | GGTGCTTAAAATTTATTATC+TGG | + | chr6_2:53391140-53391159 | Msa0919970:three_prime_UTR | 25.0% |
| ! | AATTTATTATCTGGTAGCAT+AGG | + | chr6_2:53391149-53391168 | Msa0919970:three_prime_UTR | 25.0% |
| !! | GTGTGAAGTATACTTGTTTT+GGG | - | chr6_2:53390607-53390626 | None:intergenic | 30.0% |
| ! | TGTGTGAAGTATACTTGTTT+TGG | - | chr6_2:53390608-53390627 | None:intergenic | 30.0% |
| ATCCTGAAGGAAAAAAAGAT+TGG | + | chr6_2:53390630-53390649 | Msa0919970:CDS | 30.0% | |
| !!! | AACCAATCTTTTTTTCCTTC+AGG | - | chr6_2:53390635-53390654 | None:intergenic | 30.0% |
| TGAAGGAAAAAAAGATTGGT+TGG | + | chr6_2:53390634-53390653 | Msa0919970:CDS | 30.0% | |
| ! | ATTGTGTGATTGATTCATCT+TGG | - | chr6_2:53390733-53390752 | None:intergenic | 30.0% |
| ATTGCAAATGACAATCTTGA+AGG | + | chr6_2:53390764-53390783 | Msa0919970:CDS | 30.0% | |
| TGATGAAACATATTCTGACT+AGG | + | chr6_2:53390905-53390924 | Msa0919970:intron | 30.0% | |
| ! | ATTTTCTACCCTCTTATTCT+TGG | + | chr6_2:53390939-53390958 | Msa0919970:intron | 30.0% |
| TAAGTCATCCAAGAATAAGA+GGG | - | chr6_2:53390950-53390969 | None:intergenic | 30.0% | |
| ATAAGTCATCCAAGAATAAG+AGG | - | chr6_2:53390951-53390970 | None:intergenic | 30.0% | |
| TTGTATTGTTCACGAATGTT+AGG | + | chr6_2:53391012-53391031 | Msa0919970:intron | 30.0% | |
| ATCTTCTCAAACAAATCCAA+AGG | + | chr6_2:53391092-53391111 | Msa0919970:three_prime_UTR | 30.0% | |
| AACAAATCCAAAGGATTTCA+AGG | + | chr6_2:53391101-53391120 | Msa0919970:three_prime_UTR | 30.0% | |
| ! | GTGATTGATTCATCTTGGTA+TGG | - | chr6_2:53390728-53390747 | None:intergenic | 35.0% |
| !! | GGAAGTCTTGTTGATTTAGT+TGG | + | chr6_2:53390785-53390804 | Msa0919970:CDS | 35.0% |
| TGATTTAGTTGGAGAAGTTG+AGG | + | chr6_2:53390796-53390815 | Msa0919970:CDS | 35.0% | |
| TACTTCACACAGTATCCTGA+AGG | + | chr6_2:53390617-53390636 | Msa0919970:CDS | 40.0% | |
| TGGTATGGAACTTCTTTCTC+CGG | - | chr6_2:53390713-53390732 | None:intergenic | 40.0% | |
| AAGGTAACCTCATCCAAGAA+AGG | - | chr6_2:53390833-53390852 | None:intergenic | 40.0% | |
| TGGATGAGGTTACCTTTGAA+TGG | + | chr6_2:53390837-53390856 | Msa0919970:intron | 40.0% | |
| GGATGAGGTTACCTTTGAAT+GGG | + | chr6_2:53390838-53390857 | Msa0919970:intron | 40.0% | |
| TGTTCACGAATGTTAGGTAG+AGG | + | chr6_2:53391018-53391037 | Msa0919970:intron | 40.0% | |
| GTTTCGTGCGGTTGTTTAAA+GGG | + | chr6_2:53391171-53391190 | Msa0919970:three_prime_UTR | 40.0% | |
| GTTGGCTGTGATTAAGACCA+AGG | + | chr6_2:53390652-53390671 | Msa0919970:CDS | 45.0% | |
| TTGAATTGTGCTTCTCGCCT+TGG | - | chr6_2:53390672-53390691 | None:intergenic | 45.0% | |
| ! | GGAGAAGTTGAGGAAGCATA+CGG | + | chr6_2:53390806-53390825 | Msa0919970:CDS | 45.0% |
| ATACGGTCCTTTCTTGGATG+AGG | + | chr6_2:53390823-53390842 | Msa0919970:CDS | 45.0% | |
| CGAATGTTAGGTAGAGGTAG+AGG | + | chr6_2:53391024-53391043 | Msa0919970:intron | 45.0% | |
| GGTTTCGTGCGGTTGTTTAA+AGG | + | chr6_2:53391170-53391189 | Msa0919970:three_prime_UTR | 45.0% | |
| ! | GGAAGCATACGGTCCTTTCT+TGG | + | chr6_2:53390817-53390836 | Msa0919970:CDS | 50.0% |
| TTAGGTAGAGGTAGAGGCAG+CGG | + | chr6_2:53391030-53391049 | Msa0919970:intron | 50.0% | |
| CTGGTAGCATAGGTTTCGTG+CGG | + | chr6_2:53391159-53391178 | Msa0919970:three_prime_UTR | 50.0% | |
| GTGCGGTTGTTTAAAGGGCG+TGG | + | chr6_2:53391176-53391195 | Msa0919970:three_prime_UTR | 55.0% | |
| AGAGGTAGAGGCAGCGGAAG+AGG | + | chr6_2:53391036-53391055 | Msa0919970:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 53390602 | 53391215 | 53390602 | ID=Msa0919970;Name=Msa0919970 |
| chr6_2 | mRNA | 53390602 | 53391215 | 53390602 | ID=Msa0919970-mRNA-1;Parent=Msa0919970;Name=Msa0919970-mRNA-1;_AED=0.19;_eAED=0.57;_QI=0|0|0.5|1|0|0|2|146|92 |
| chr6_2 | exon | 53390602 | 53390844 | 53390602 | ID=Msa0919970-mRNA-1:exon:7976;Parent=Msa0919970-mRNA-1 |
| chr6_2 | exon | 53391034 | 53391215 | 53391034 | ID=Msa0919970-mRNA-1:exon:7977;Parent=Msa0919970-mRNA-1 |
| chr6_2 | CDS | 53390602 | 53390844 | 53390602 | ID=Msa0919970-mRNA-1:cds;Parent=Msa0919970-mRNA-1 |
| chr6_2 | CDS | 53391034 | 53391069 | 53391034 | ID=Msa0919970-mRNA-1:cds;Parent=Msa0919970-mRNA-1 |
| chr6_2 | three_prime_UTR | 53391070 | 53391215 | 53391070 | ID=Msa0919970-mRNA-1:three_prime_utr;Parent=Msa0919970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
