AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1070950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 88 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCAGGGTTGACGATTC+AGG 0.139753 7_2:+67275719 Msa1070950:five_prime_UTR
TAAGATTACATGTATCATTT+TGG 0.226865 7_2:-67274172 None:intergenic
TGTTTCAATTTCTCTACTTT+GGG 0.267804 7_2:+67274350 Msa1070950:five_prime_UTR
CACCACTTCAATGCATGAAT+TGG 0.272830 7_2:-67274313 None:intergenic
GGAAGCATATGGTCCTTTCT+TGG 0.309175 7_2:+67276065 Msa1070950:CDS
ATTGGGATAAGAAAGAAAAT+TGG 0.309564 7_2:-67274295 None:intergenic
TTGTTTCAATTTCTCTACTT+TGG 0.314070 7_2:+67274349 Msa1070950:five_prime_UTR
TTGGGATAAGAAAGAAAATT+GGG 0.319969 7_2:-67274294 None:intergenic
ATTAATGGTGCTGCAAAGTT+TGG 0.320369 7_2:+67275810 Msa1070950:exon
AGGGAGTATGTTCTGATCTT+AGG 0.331231 7_2:-67274081 None:intergenic
GGAAGTCTTGTTGATTTAGT+TGG 0.335009 7_2:+67276033 Msa1070950:CDS
GGTGCTTAAAGTTTATTATC+TGG 0.337900 7_2:+67276402 Msa1070950:three_prime_UTR
CAGTTAAGACCGAGGCTTCT+TGG 0.340085 7_2:+67275701 Msa1070950:five_prime_UTR
TCAAAGGGTTATGGTGGCTT+TGG 0.352518 7_2:-67274133 None:intergenic
AATTTCTGCGTAGGAATATT+CGG 0.369204 7_2:+67275455 Msa1070950:intron
AAAATACCTTGAAATCCTTT+GGG 0.373281 7_2:-67276369 None:intergenic
TGATGAAGTTCAAAGGGTTA+TGG 0.383423 7_2:-67274142 None:intergenic
GCAAGAACAAGAAGAATAGA+AGG 0.386139 7_2:+67274545 Msa1070950:five_prime_UTR
ATTTGTGTTCCAGATTGTTT+AGG 0.397267 7_2:+67275674 Msa1070950:intron
GGTTTCGTGCGGTTGTTTAG+AGG 0.398949 7_2:+67276432 Msa1070950:three_prime_UTR
TGGATGAGGTTACCTTTGAA+TGG 0.410546 7_2:+67276085 Msa1070950:CDS
TTGAATTGAGCTTCTCGCCT+TGG 0.420399 7_2:-67275917 None:intergenic
TAAGACCGAGGCTTCTTGGC+AGG 0.423322 7_2:+67275705 Msa1070950:five_prime_UTR
AATTGCTGCGTAGGAATATT+CGG 0.425916 7_2:+67275577 Msa1070950:five_prime_UTR
TTGGCAGGGTTGACGATTCA+GGG 0.436084 7_2:+67275720 Msa1070950:five_prime_UTR
CTGTAGGTTCGGTTGGGACT+AGG 0.439250 7_2:-67274255 None:intergenic
CGTGTTCGTGCATTTGAAAC+GGG 0.440856 7_2:+67275533 Msa1070950:five_prime_UTR
TTTGGGTTAACATGAAGCTT+AGG 0.441413 7_2:+67274367 Msa1070950:five_prime_UTR
TGGTTCATCCCCACTTGATA+TGG 0.445421 7_2:+67274605 Msa1070950:five_prime_UTR
TGGTATGGAACTTCTTTCTC+CGG 0.445848 7_2:-67275958 None:intergenic
ACCACTTCAATGCATGAATT+GGG 0.454441 7_2:-67274312 None:intergenic
GGATGAGGTTACCTTTGAAT+GGG 0.455125 7_2:+67276086 Msa1070950:CDS
ATTGCAAATGACAATCTTGA+AGG 0.456999 7_2:+67276012 Msa1070950:CDS
AGAGGTAGAGGCAGCGGAAG+AGG 0.457632 7_2:+67276298 Msa1070950:three_prime_UTR
AGAATAGAAGGAGAAATTGA+AGG 0.460014 7_2:+67274557 Msa1070950:five_prime_UTR
CAAAGATTGATGAAGTTCAA+AGG 0.462763 7_2:-67274149 None:intergenic
GAAAGAAAATTGGGGAAAGA+AGG 0.470391 7_2:-67274285 None:intergenic
AACAAACCCAAAGGATTTCA+AGG 0.473412 7_2:+67276363 Msa1070950:three_prime_UTR
ATTGTGTGATTGATTCATCT+TGG 0.474013 7_2:-67275978 None:intergenic
GCGTGTTCGTGCATTTGAAA+CGG 0.477104 7_2:+67275532 Msa1070950:five_prime_UTR
AAAGATTGATGAAGTTCAAA+GGG 0.477967 7_2:-67274148 None:intergenic
ATATGGTCCTTTCTTGGATG+AGG 0.478415 7_2:+67276071 Msa1070950:CDS
GTGCGGTTGTTTAGAGGGTG+TGG 0.481036 7_2:+67276438 Msa1070950:three_prime_UTR
AATATCTTTATCCCATTCAA+AGG 0.483583 7_2:-67276097 None:intergenic
GTTTCGTGCGGTTGTTTAGA+GGG 0.489116 7_2:+67276433 Msa1070950:three_prime_UTR
GTGATTGATTCATCTTGGTA+TGG 0.505565 7_2:-67275973 None:intergenic
CCTCACTCCTTTCTATCTCT+CGG 0.506825 7_2:+67274101 Msa1070950:five_prime_UTR
GTAGGACTGTAGGTTCGGTT+GGG 0.513758 7_2:-67274261 None:intergenic
AGTTTATTATCTGGTAGCAT+AGG 0.515942 7_2:+67276411 Msa1070950:three_prime_UTR
GAATAGAAGGAGAAATTGAA+GGG 0.517096 7_2:+67274558 Msa1070950:five_prime_UTR
TGTAGGACTGTAGGTTCGGT+TGG 0.518008 7_2:-67274262 None:intergenic
TCAGAGCTCTTGTTCATCAA+TGG 0.520698 7_2:+67274582 Msa1070950:five_prime_UTR
GGGTTATGGTGGCTTTGGAA+TGG 0.520808 7_2:-67274128 None:intergenic
TGATTTAGTTGGAGAAGTTG+AGG 0.526516 7_2:+67276044 Msa1070950:CDS
CGAATGTTAGGTAGAGGTAG+AGG 0.532296 7_2:+67276286 Msa1070950:intron
TTAGGTAGAGGTAGAGGCAG+CGG 0.532621 7_2:+67276292 Msa1070950:intron
AAGCTTGAATTATTGAGCGT+TGG 0.534500 7_2:+67274518 Msa1070950:five_prime_UTR
GGAGAAGTTGAGGAAGCATA+TGG 0.534990 7_2:+67276054 Msa1070950:CDS
AAGGTGTAGGACTGTAGGTT+CGG 0.544407 7_2:-67274266 None:intergenic
ACGTTCTGTAATTGCTGCGT+AGG 0.545882 7_2:+67275568 Msa1070950:five_prime_UTR
TCAACCCTGCCAAGAAGCCT+CGG 0.549766 7_2:-67275710 None:intergenic
TGGATGGCCGAGAGATAGAA+AGG 0.550376 7_2:-67274108 None:intergenic
AAGACCGAGGCTTCTTGGCA+GGG 0.550613 7_2:+67275706 Msa1070950:five_prime_UTR
TATGGTGGCTTTGGAATGGA+TGG 0.550702 7_2:-67274124 None:intergenic
CAATTCATGCATTGAAGTGG+TGG 0.554056 7_2:+67274314 Msa1070950:five_prime_UTR
AACTTTAGAAGATAGTAAGC+CGG 0.555137 7_2:+67275939 Msa1070950:CDS
GAAAGAAGGTGTAGGACTGT+AGG 0.562934 7_2:-67274271 None:intergenic
AAGGTAACCTCATCCAAGAA+AGG 0.563434 7_2:-67276078 None:intergenic
TCCCAATTCATGCATTGAAG+TGG 0.578368 7_2:+67274311 Msa1070950:five_prime_UTR
TGAAGTTCAAAGGGTTATGG+TGG 0.583833 7_2:-67274139 None:intergenic
AGTAGAGAAATTGAAACAAG+AGG 0.594700 7_2:-67274345 None:intergenic
GAGCTCTTGTTCATCAATGG+TGG 0.600072 7_2:+67274585 Msa1070950:five_prime_UTR
CCGAGAGATAGAAAGGAGTG+AGG 0.602325 7_2:-67274101 None:intergenic
AGAGAACTCACCATATCAAG+TGG 0.605853 7_2:-67274615 None:intergenic
CGAGAGATAGAAAGGAGTGA+GGG 0.607309 7_2:-67274100 None:intergenic
TGATGAAACATATTCTGACT+AGG 0.610583 7_2:+67276167 Msa1070950:CDS
GTTGGCTGTGATTAAGACCA+AGG 0.613774 7_2:+67275900 Msa1070950:CDS
TACTTCACACAGTATCCTGA+AGG 0.615568 7_2:+67275865 Msa1070950:CDS
GAGAACTCACCATATCAAGT+GGG 0.622028 7_2:-67274614 None:intergenic
TGGCAGGGTTGACGATTCAG+GGG 0.627660 7_2:+67275721 Msa1070950:five_prime_UTR
TGGGATAAGAAAGAAAATTG+GGG 0.635478 7_2:-67274293 None:intergenic
CTGGTAGCATAGGTTTCGTG+CGG 0.636336 7_2:+67276421 Msa1070950:three_prime_UTR
GGTTCGGTTGGGACTAGGAG+AGG 0.638556 7_2:-67274250 None:intergenic
ATCTTCTCAAACAAACCCAA+AGG 0.644202 7_2:+67276354 Msa1070950:three_prime_UTR
TCATGCATTGAAGTGGTGGA+AGG 0.653671 7_2:+67274318 Msa1070950:five_prime_UTR
TAGGCAGACAGTTAAGACCG+AGG 0.682894 7_2:+67275693 Msa1070950:five_prime_UTR
AGAACTCACCATATCAAGTG+GGG 0.690846 7_2:-67274613 None:intergenic
AAATTGGGGAAAGAAGGTGT+AGG 0.709182 7_2:-67274279 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAAAATACTAATTTTAT+CGG - chr7_2:67275400-67275419 None:intergenic 10.0%
!! ATTAATTCACATAAAAAATG+AGG - chr7_2:67274201-67274220 None:intergenic 15.0%
!!! TAAGATTACATGTATCATTT+TGG - chr7_2:67274175-67274194 None:intergenic 20.0%
!!! TTTTTATGTGAATTAATGTG+TGG + chr7_2:67274204-67274223 Msa1070950:five_prime_UTR 20.0%
!! TCAACTCCTTAATTAATTAA+TGG + chr7_2:67274466-67274485 Msa1070950:intron 20.0%
!! ATGCAACCATTAATTAATTA+AGG - chr7_2:67274475-67274494 None:intergenic 20.0%
!!! AAATTTTGTCTATTTTCCAA+TGG - chr7_2:67274942-67274961 None:intergenic 20.0%
!! CAAAATAACTTAAAAGTCTT+TGG - chr7_2:67275255-67275274 None:intergenic 20.0%
!!! CAAGGTATTTTTATGATATA+AGG + chr7_2:67276381-67276400 Msa1070950:three_prime_UTR 20.0%
! AAAGATTGATGAAGTTCAAA+GGG - chr7_2:67274151-67274170 None:intergenic 25.0%
! TTGGGATAAGAAAGAAAATT+GGG - chr7_2:67274297-67274316 None:intergenic 25.0%
! ATTGGGATAAGAAAGAAAAT+TGG - chr7_2:67274298-67274317 None:intergenic 25.0%
! TTGTTTCAATTTCTCTACTT+TGG + chr7_2:67274349-67274368 Msa1070950:five_prime_UTR 25.0%
! TGTTTCAATTTCTCTACTTT+GGG + chr7_2:67274350-67274369 Msa1070950:five_prime_UTR 25.0%
! TTAATTAAGGAGTTGAAAGT+GGG - chr7_2:67274462-67274481 None:intergenic 25.0%
! ATTAATTAAGGAGTTGAAAG+TGG - chr7_2:67274463-67274482 None:intergenic 25.0%
! AATTAATTAATGGTTGCATG+TGG + chr7_2:67274476-67274495 Msa1070950:intron 25.0%
!!! TTAGAGTTGAATTTGTTGTT+TGG + chr7_2:67274659-67274678 Msa1070950:intron 25.0%
! ATTAATCTTGCACATAACAT+GGG - chr7_2:67274736-67274755 None:intergenic 25.0%
! AATTAATCTTGCACATAACA+TGG - chr7_2:67274737-67274756 None:intergenic 25.0%
! TTATGTGCAAGATTAATTCA+TGG + chr7_2:67274739-67274758 Msa1070950:intron 25.0%
! AGATTAATTCATGGAACAAA+TGG + chr7_2:67274748-67274767 Msa1070950:intron 25.0%
! TTTAACGATGATAATCCATT+TGG - chr7_2:67274805-67274824 None:intergenic 25.0%
! ATCATCGTTAAAAGTGATTA+TGG + chr7_2:67274813-67274832 Msa1070950:intron 25.0%
! AATGTCAGTTAAAATTCGTA+TGG + chr7_2:67274853-67274872 Msa1070950:intron 25.0%
! GACAAAATTTAACACGAAAT+TGG + chr7_2:67274952-67274971 Msa1070950:intron 25.0%
!! GACAGAAATTAACTTGAAAT+AGG + chr7_2:67275133-67275152 Msa1070950:intron 25.0%
! CTACTTGTAAATGTAACTTT+CGG + chr7_2:67275202-67275221 Msa1070950:intron 25.0%
! TTTATTACAAATTTCTGCGT+AGG + chr7_2:67275446-67275465 Msa1070950:intron 25.0%
! TTGAACATCAAAGGAAAAAA+AGG - chr7_2:67275618-67275637 None:intergenic 25.0%
! ACCATTAATGTAAAAATCCA+GGG - chr7_2:67275799-67275818 None:intergenic 25.0%
! AATATCTTTATCCCATTCAA+AGG - chr7_2:67276100-67276119 None:intergenic 25.0%
! AAAATACCTTGAAATCCTTT+GGG - chr7_2:67276372-67276391 None:intergenic 25.0%
! AAAAATACCTTGAAATCCTT+TGG - chr7_2:67276373-67276392 None:intergenic 25.0%
!!! GTGTTTTTGACTTTGTTTAA+TGG + chr7_2:67276555-67276574 Msa1070950:three_prime_UTR 25.0%
CAAAGATTGATGAAGTTCAA+AGG - chr7_2:67274152-67274171 None:intergenic 30.0%
TGGGATAAGAAAGAAAATTG+GGG - chr7_2:67274296-67274315 None:intergenic 30.0%
AGTAGAGAAATTGAAACAAG+AGG - chr7_2:67274348-67274367 None:intergenic 30.0%
AGAATAGAAGGAGAAATTGA+AGG + chr7_2:67274557-67274576 Msa1070950:five_prime_UTR 30.0%
GAATAGAAGGAGAAATTGAA+GGG + chr7_2:67274558-67274577 Msa1070950:five_prime_UTR 30.0%
!! AATTTGTTGTTTGGAGGTAA+TGG + chr7_2:67274668-67274687 Msa1070950:intron 30.0%
!! ATTTGTTGTTTGGAGGTAAT+GGG + chr7_2:67274669-67274688 Msa1070950:intron 30.0%
TCGTTAAAAGTGATTATGGA+TGG + chr7_2:67274817-67274836 Msa1070950:intron 30.0%
! TACGTGATTCATTTTGTAGT+GGG + chr7_2:67274909-67274928 Msa1070950:intron 30.0%
! ATTTAACACGAAATTGGCTT+TGG + chr7_2:67274958-67274977 Msa1070950:intron 30.0%
TCTATGTTCATACATCACTT+TGG + chr7_2:67275084-67275103 Msa1070950:intron 30.0%
AATTTCTGTCTTCTTTCTCA+TGG - chr7_2:67275123-67275142 None:intergenic 30.0%
TGAGTAGTCTAAGAATACAA+AGG + chr7_2:67275283-67275302 Msa1070950:intron 30.0%
AATTTCTGCGTAGGAATATT+CGG + chr7_2:67275455-67275474 Msa1070950:intron 30.0%
! CAATCTGGAACACAAATAAA+AGG - chr7_2:67275671-67275690 None:intergenic 30.0%
ATTTGTGTTCCAGATTGTTT+AGG + chr7_2:67275674-67275693 Msa1070950:intron 30.0%
!!! GTTTTATTTTATACCAACCC+TGG + chr7_2:67275779-67275798 Msa1070950:five_prime_UTR 30.0%
TTAATGTAAAAATCCAGGGT+TGG - chr7_2:67275795-67275814 None:intergenic 30.0%
! ACCCTGGATTTTTACATTAA+TGG + chr7_2:67275795-67275814 Msa1070950:exon 30.0%
CACCATTAATGTAAAAATCC+AGG - chr7_2:67275800-67275819 None:intergenic 30.0%
!! GTGTGAAGTATACTTGTTTT+TGG - chr7_2:67275855-67275874 None:intergenic 30.0%
ATCCTGAAGGAAAAAAAGAT+TGG + chr7_2:67275878-67275897 Msa1070950:CDS 30.0%
!!! AACCAATCTTTTTTTCCTTC+AGG - chr7_2:67275883-67275902 None:intergenic 30.0%
TGAAGGAAAAAAAGATTGGT+TGG + chr7_2:67275882-67275901 Msa1070950:CDS 30.0%
! AACTTTAGAAGATAGTAAGC+CGG + chr7_2:67275939-67275958 Msa1070950:CDS 30.0%
! ATTGTGTGATTGATTCATCT+TGG - chr7_2:67275981-67276000 None:intergenic 30.0%
ATTGCAAATGACAATCTTGA+AGG + chr7_2:67276012-67276031 Msa1070950:CDS 30.0%
ATCATCATCATCATCATCAT+CGG - chr7_2:67276145-67276164 None:intergenic 30.0%
TGATGAAACATATTCTGACT+AGG + chr7_2:67276167-67276186 Msa1070950:CDS 30.0%
TAAGTCATGCAAGAATAAGA+GGG - chr7_2:67276212-67276231 None:intergenic 30.0%
ATAAGTCATGCAAGAATAAG+AGG - chr7_2:67276213-67276232 None:intergenic 30.0%
TTGTATTGTTCACGAATGTT+AGG + chr7_2:67276274-67276293 Msa1070950:intron 30.0%
GGTGCTTAAAGTTTATTATC+TGG + chr7_2:67276402-67276421 Msa1070950:three_prime_UTR 30.0%
AGTTTATTATCTGGTAGCAT+AGG + chr7_2:67276411-67276430 Msa1070950:three_prime_UTR 30.0%
!! AGCACTTTTTGTTTCAGTTT+TGG + chr7_2:67276462-67276481 Msa1070950:three_prime_UTR 30.0%
TGATGAAGTTCAAAGGGTTA+TGG - chr7_2:67274145-67274164 None:intergenic 35.0%
GAAAGAAAATTGGGGAAAGA+AGG - chr7_2:67274288-67274307 None:intergenic 35.0%
ACCACTTCAATGCATGAATT+GGG - chr7_2:67274315-67274334 None:intergenic 35.0%
! TTTGGGTTAACATGAAGCTT+AGG + chr7_2:67274367-67274386 Msa1070950:five_prime_UTR 35.0%
AAGCTTGAATTATTGAGCGT+TGG + chr7_2:67274518-67274537 Msa1070950:five_prime_UTR 35.0%
GCAAGAACAAGAAGAATAGA+AGG + chr7_2:67274545-67274564 Msa1070950:five_prime_UTR 35.0%
!! GAGTTGAATTTGTTGTTTGG+AGG + chr7_2:67274662-67274681 Msa1070950:intron 35.0%
!! TCATGGAACAAATGGTTTTG+AGG + chr7_2:67274756-67274775 Msa1070950:intron 35.0%
! GTACGTGATTCATTTTGTAG+TGG + chr7_2:67274908-67274927 Msa1070950:intron 35.0%
!! ACTTGAAATAGGCTTTGAGA+TGG + chr7_2:67275144-67275163 Msa1070950:intron 35.0%
AATTGCTGCGTAGGAATATT+CGG + chr7_2:67275577-67275596 Msa1070950:five_prime_UTR 35.0%
! GAATATTCGGTAAAGTACTC+AGG + chr7_2:67275468-67275487 Msa1070950:five_prime_UTR 35.0%
! AAGCACAAGTTGAACATCAA+AGG - chr7_2:67275627-67275646 None:intergenic 35.0%
!! ATTAATGGTGCTGCAAAGTT+TGG + chr7_2:67275810-67275829 Msa1070950:exon 35.0%
! GTGATTGATTCATCTTGGTA+TGG - chr7_2:67275976-67275995 None:intergenic 35.0%
!! GGAAGTCTTGTTGATTTAGT+TGG + chr7_2:67276033-67276052 Msa1070950:CDS 35.0%
TGATTTAGTTGGAGAAGTTG+AGG + chr7_2:67276044-67276063 Msa1070950:CDS 35.0%
ATCTTCTCAAACAAACCCAA+AGG + chr7_2:67276354-67276373 Msa1070950:three_prime_UTR 35.0%
AACAAACCCAAAGGATTTCA+AGG + chr7_2:67276363-67276382 Msa1070950:three_prime_UTR 35.0%
TGAAGTTCAAAGGGTTATGG+TGG - chr7_2:67274142-67274161 None:intergenic 40.0%
! AAATTGGGGAAAGAAGGTGT+AGG - chr7_2:67274282-67274301 None:intergenic 40.0%
TCCCAATTCATGCATTGAAG+TGG + chr7_2:67274311-67274330 Msa1070950:five_prime_UTR 40.0%
CACCACTTCAATGCATGAAT+TGG - chr7_2:67274316-67274335 None:intergenic 40.0%
CAATTCATGCATTGAAGTGG+TGG + chr7_2:67274314-67274333 Msa1070950:five_prime_UTR 40.0%
! AAGTTTCGGTGCAAGAAGAA+TGG - chr7_2:67274426-67274445 None:intergenic 40.0%
TCAGAGCTCTTGTTCATCAA+TGG + chr7_2:67274582-67274601 Msa1070950:five_prime_UTR 40.0%
AGAACTCACCATATCAAGTG+GGG - chr7_2:67274616-67274635 None:intergenic 40.0%
GAGAACTCACCATATCAAGT+GGG - chr7_2:67274617-67274636 None:intergenic 40.0%
AGAGAACTCACCATATCAAG+TGG - chr7_2:67274618-67274637 None:intergenic 40.0%
!! TTGTTTGGAGGTAATGGGTT+TGG + chr7_2:67274674-67274693 Msa1070950:intron 40.0%
!! TGTTTGGAGGTAATGGGTTT+GGG + chr7_2:67274675-67274694 Msa1070950:intron 40.0%
GGGACATGTTTGAGAAAAGT+AGG + chr7_2:67274695-67274714 Msa1070950:intron 40.0%
GTGAAGCAATGACATCCAAA+TGG + chr7_2:67274787-67274806 Msa1070950:intron 40.0%
AATTCGTATGGACCTGTTGT+CGG + chr7_2:67274865-67274884 Msa1070950:intron 40.0%
! ATGACAGTTTTACCGACAAC+AGG - chr7_2:67274880-67274899 None:intergenic 40.0%
! TGTAGTGGGAATTGAACCAT+TGG + chr7_2:67274923-67274942 Msa1070950:intron 40.0%
! TGTTCTTGCGTTTTCGATCT+TGG + chr7_2:67275505-67275524 Msa1070950:five_prime_UTR 40.0%
AACTGTCTGCCTAAACAATC+TGG - chr7_2:67275686-67275705 None:intergenic 40.0%
TACTTCACACAGTATCCTGA+AGG + chr7_2:67275865-67275884 Msa1070950:CDS 40.0%
TGGTATGGAACTTCTTTCTC+CGG - chr7_2:67275961-67275980 None:intergenic 40.0%
ATATGGTCCTTTCTTGGATG+AGG + chr7_2:67276071-67276090 Msa1070950:CDS 40.0%
AAGGTAACCTCATCCAAGAA+AGG - chr7_2:67276081-67276100 None:intergenic 40.0%
TGGATGAGGTTACCTTTGAA+TGG + chr7_2:67276085-67276104 Msa1070950:CDS 40.0%
GGATGAGGTTACCTTTGAAT+GGG + chr7_2:67276086-67276105 Msa1070950:CDS 40.0%
TGTTCACGAATGTTAGGTAG+AGG + chr7_2:67276280-67276299 Msa1070950:intron 40.0%
CGAGAGATAGAAAGGAGTGA+GGG - chr7_2:67274103-67274122 None:intergenic 45.0%
CCTCACTCCTTTCTATCTCT+CGG + chr7_2:67274101-67274120 Msa1070950:five_prime_UTR 45.0%
!! TATGGTGGCTTTGGAATGGA+TGG - chr7_2:67274127-67274146 None:intergenic 45.0%
TCAAAGGGTTATGGTGGCTT+TGG - chr7_2:67274136-67274155 None:intergenic 45.0%
AAGGTGTAGGACTGTAGGTT+CGG - chr7_2:67274269-67274288 None:intergenic 45.0%
! GAAAGAAGGTGTAGGACTGT+AGG - chr7_2:67274274-67274293 None:intergenic 45.0%
! TCATGCATTGAAGTGGTGGA+AGG + chr7_2:67274318-67274337 Msa1070950:five_prime_UTR 45.0%
GCTGAACAGGGGTTAAGTTT+CGG - chr7_2:67274440-67274459 None:intergenic 45.0%
!! AGTTGAAAGTGGGCTGAACA+GGG - chr7_2:67274452-67274471 None:intergenic 45.0%
! GCATGTGGATTTTCTTGCTC+AGG + chr7_2:67274491-67274510 Msa1070950:intron 45.0%
GAGCTCTTGTTCATCAATGG+TGG + chr7_2:67274585-67274604 Msa1070950:five_prime_UTR 45.0%
TGGTTCATCCCCACTTGATA+TGG + chr7_2:67274605-67274624 Msa1070950:five_prime_UTR 45.0%
TCGGCAAGCACATTCGAATT+TGG + chr7_2:67275221-67275240 Msa1070950:intron 45.0%
CGGCAAGCACATTCGAATTT+GGG + chr7_2:67275222-67275241 Msa1070950:intron 45.0%
GCGTGTTCGTGCATTTGAAA+CGG + chr7_2:67275532-67275551 Msa1070950:five_prime_UTR 45.0%
! CGTGTTCGTGCATTTGAAAC+GGG + chr7_2:67275533-67275552 Msa1070950:five_prime_UTR 45.0%
ACGTTCTGTAATTGCTGCGT+AGG + chr7_2:67275568-67275587 Msa1070950:five_prime_UTR 45.0%
GTTGGCTGTGATTAAGACCA+AGG + chr7_2:67275900-67275919 Msa1070950:CDS 45.0%
TTGAATTGAGCTTCTCGCCT+TGG - chr7_2:67275920-67275939 None:intergenic 45.0%
! GGAGAAGTTGAGGAAGCATA+TGG + chr7_2:67276054-67276073 Msa1070950:CDS 45.0%
! GGAAGCATATGGTCCTTTCT+TGG + chr7_2:67276065-67276084 Msa1070950:CDS 45.0%
CGAATGTTAGGTAGAGGTAG+AGG + chr7_2:67276286-67276305 Msa1070950:intron 45.0%
GTTTCGTGCGGTTGTTTAGA+GGG + chr7_2:67276433-67276452 Msa1070950:three_prime_UTR 45.0%
CCGAGAGATAGAAAGGAGTG+AGG - chr7_2:67274104-67274123 None:intergenic 50.0%
TGGATGGCCGAGAGATAGAA+AGG - chr7_2:67274111-67274130 None:intergenic 50.0%
!! GGGTTATGGTGGCTTTGGAA+TGG - chr7_2:67274131-67274150 None:intergenic 50.0%
! GTAGGACTGTAGGTTCGGTT+GGG - chr7_2:67274264-67274283 None:intergenic 50.0%
! TGTAGGACTGTAGGTTCGGT+TGG - chr7_2:67274265-67274284 None:intergenic 50.0%
!! GTTGAAAGTGGGCTGAACAG+GGG - chr7_2:67274451-67274470 None:intergenic 50.0%
!! GAGTTGAAAGTGGGCTGAAC+AGG - chr7_2:67274453-67274472 None:intergenic 50.0%
TAGGCAGACAGTTAAGACCG+AGG + chr7_2:67275693-67275712 Msa1070950:five_prime_UTR 50.0%
CAGTTAAGACCGAGGCTTCT+TGG + chr7_2:67275701-67275720 Msa1070950:five_prime_UTR 50.0%
TTGGCAGGGTTGACGATTCA+GGG + chr7_2:67275720-67275739 Msa1070950:five_prime_UTR 50.0%
TTAGGTAGAGGTAGAGGCAG+CGG + chr7_2:67276292-67276311 Msa1070950:intron 50.0%
CTGGTAGCATAGGTTTCGTG+CGG + chr7_2:67276421-67276440 Msa1070950:three_prime_UTR 50.0%
GGTTTCGTGCGGTTGTTTAG+AGG + chr7_2:67276432-67276451 Msa1070950:three_prime_UTR 50.0%
! CTGTAGGTTCGGTTGGGACT+AGG - chr7_2:67274258-67274277 None:intergenic 55.0%
TAAGACCGAGGCTTCTTGGC+AGG + chr7_2:67275705-67275724 Msa1070950:five_prime_UTR 55.0%
AAGACCGAGGCTTCTTGGCA+GGG + chr7_2:67275706-67275725 Msa1070950:five_prime_UTR 55.0%
TCAACCCTGCCAAGAAGCCT+CGG - chr7_2:67275713-67275732 None:intergenic 55.0%
CTTGGCAGGGTTGACGATTC+AGG + chr7_2:67275719-67275738 Msa1070950:five_prime_UTR 55.0%
TGGCAGGGTTGACGATTCAG+GGG + chr7_2:67275721-67275740 Msa1070950:five_prime_UTR 55.0%
GTGCGGTTGTTTAGAGGGTG+TGG + chr7_2:67276438-67276457 Msa1070950:three_prime_UTR 55.0%
! GGTTCGGTTGGGACTAGGAG+AGG - chr7_2:67274253-67274272 None:intergenic 60.0%
AGAGGTAGAGGCAGCGGAAG+AGG + chr7_2:67276298-67276317 Msa1070950:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 67274082 67276586 67274082 ID=Msa1070950;Name=Msa1070950
chr7_2 mRNA 67274082 67276586 67274082 ID=Msa1070950-mRNA-1;Parent=Msa1070950;Name=Msa1070950-mRNA-1;_AED=0.04;_eAED=0.04;_QI=679|1|1|1|0|0.2|5|291|124
chr7_2 exon 67274082 67274388 67274082 ID=Msa1070950-mRNA-1:exon:14554;Parent=Msa1070950-mRNA-1
chr7_2 exon 67274513 67274626 67274513 ID=Msa1070950-mRNA-1:exon:14555;Parent=Msa1070950-mRNA-1
chr7_2 exon 67275468 67275598 67275468 ID=Msa1070950-mRNA-1:exon:14556;Parent=Msa1070950-mRNA-1
chr7_2 exon 67275687 67276188 67275687 ID=Msa1070950-mRNA-1:exon:14557;Parent=Msa1070950-mRNA-1
chr7_2 exon 67276296 67276586 67276296 ID=Msa1070950-mRNA-1:exon:14558;Parent=Msa1070950-mRNA-1
chr7_2 five_prime_UTR 67274082 67274388 67274082 ID=Msa1070950-mRNA-1:five_prime_utr;Parent=Msa1070950-mRNA-1
chr7_2 five_prime_UTR 67274513 67274626 67274513 ID=Msa1070950-mRNA-1:five_prime_utr;Parent=Msa1070950-mRNA-1
chr7_2 five_prime_UTR 67275468 67275598 67275468 ID=Msa1070950-mRNA-1:five_prime_utr;Parent=Msa1070950-mRNA-1
chr7_2 five_prime_UTR 67275687 67275813 67275687 ID=Msa1070950-mRNA-1:five_prime_utr;Parent=Msa1070950-mRNA-1
chr7_2 CDS 67275814 67276188 67275814 ID=Msa1070950-mRNA-1:cds;Parent=Msa1070950-mRNA-1
chr7_2 three_prime_UTR 67276296 67276586 67276296 ID=Msa1070950-mRNA-1:three_prime_utr;Parent=Msa1070950-mRNA-1
Gene Sequence

>Msa1070950

ATGGTGCTGCAAAGTTTGGTTAATGATTTTTGTAAGATCCAAAAACAAGTATACTTCACACAGTATCCTGAAGGAAAAAAAGATTGGTTGGCTGTGATTAAGACCAAGGCGAGAAGCTCAATTCAAACTTTAGAAGATAGTAAGCCGGAGAAAGAAGTTCCATACCAAGATGAATCAATCACACAATCACACGTGACAATTGCAAATGACAATCTTGAAGGAAGTCTTGTTGATTTAGTTGGAGAAGTTGAGGAAGCATATGGTCCTTTCTTGGATGAGGTTACCTTTGAATGGGATAAAGATATTGAAGTAGAAGTAGAAGACAGTGCCGATGATGATGATGATGATGATGATGATGAAACATATTCTGACTAG
Protein sequence

>Msa1070950

MVLQSLVNDFCKIQKQVYFTQYPEGKKDWLAVIKTKARSSIQTLEDSKPEKEVPYQDESITQSHVTIANDNLEGSLVDLVGEVEEAYGPFLDEVTFEWDKDIEVEVEDSADDDDDDDDDETYSD*