AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0644340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0644340 MtrunA17_Chr4g0031521 98.315 712 4 3 1 712 1 704 0.0 1430
Msa0644340 MtrunA17_Chr4g0054811 39.333 839 315 23 1 710 1 774 5.69e-165 494
Msa0644340 MtrunA17_Chr7g0238501 57.843 306 89 3 407 712 290 555 1.17e-111 348
Msa0644340 MtrunA17_Chr7g0238501 31.233 365 184 8 1 346 1 317 1.89e-38 150
Msa0644340 MtrunA17_Chr1g0193691 52.226 337 137 3 397 710 555 890 1.31e-109 353
Msa0644340 MtrunA17_Chr1g0193691 38.734 395 162 14 1 346 1 364 3.10e-60 218
Msa0644340 MtrunA17_Chr4g0039061 58.966 290 83 2 423 712 238 491 6.89e-109 339
Msa0644340 MtrunA17_Chr4g0039061 67.188 64 21 0 259 322 173 236 6.01e-23 103
Msa0644340 MtrunA17_Chr4g0067301 40.809 272 133 1 432 703 244 487 3.24e-62 216
Msa0644340 MtrunA17_Chr4g0067301 49.254 67 34 0 256 322 132 198 3.16e-12 69.7
Msa0644340 MtrunA17_Chr2g0295991 40.074 272 133 2 432 703 235 476 4.10e-60 210
Msa0644340 MtrunA17_Chr2g0295991 47.945 73 38 0 259 331 137 209 9.58e-14 74.3
Msa0644340 MtrunA17_Chr8g0371321 31.786 280 149 5 433 707 115 357 2.95e-36 140
Msa0644340 MtrunA17_Chr1g0213351 31.228 285 154 5 425 704 108 355 5.24e-36 140
Msa0644340 MtrunA17_Chr1g0181901 30.693 303 147 6 435 707 114 383 5.08e-33 132
Msa0644340 MtrunA17_Chr3g0089691 32.192 292 140 11 428 704 13 261 3.03e-31 124
Msa0644340 MtrunA17_Chr7g0263281 30.824 279 151 6 434 707 108 349 4.52e-31 125
Msa0644340 MtrunA17_Chr7g0250061 28.214 280 159 5 433 707 112 354 1.92e-30 124
Msa0644340 MtrunA17_Chr3g0089711 31.849 292 141 11 428 704 99 347 2.04e-30 123
Msa0644340 MtrunA17_Chr3g0089721 31.849 292 141 11 428 704 99 347 2.32e-30 123
Msa0644340 MtrunA17_Chr1g0211251 28.929 280 157 5 433 707 117 359 7.26e-29 119
Msa0644340 MtrunA17_Chr7g0223641 30.588 255 108 6 457 703 3 196 1.73e-28 113
Msa0644340 MtrunA17_Chr2g0294291 27.636 275 157 5 434 703 113 350 3.32e-28 117
Msa0644340 MtrunA17_Chr1g0207571 30.686 277 150 7 434 704 98 338 1.06e-27 115
Msa0644340 MtrunA17_Chr1g0175381 27.208 283 161 8 436 712 118 361 9.98e-26 110
Msa0644340 MtrunA17_Chr7g0256751 31.488 289 140 10 428 701 100 345 3.88e-25 108
Msa0644340 MtrunA17_Chr7g0256771 31.839 223 103 7 435 647 109 292 1.63e-19 90.1
Msa0644340 MtrunA17_Chr4g0063051 26.814 317 155 12 409 708 57 313 1.71e-17 84.7
Msa0644340 MtrunA17_Chr7g0256761 29.891 184 103 7 533 708 20 185 4.00e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0644340 AT1G07630.1 68.283 722 159 16 1 712 1 662 0.0 927
Msa0644340 AT2G28890.1 68.428 719 155 15 1 712 1 654 0.0 920
Msa0644340 AT5G02400.1 49.790 715 277 19 19 712 21 674 0.0 613
Msa0644340 AT3G09400.1 48.016 731 280 17 1 712 1 650 0.0 563
Msa0644340 AT3G09400.2 45.821 694 276 17 1 675 1 613 6.07e-166 492
Msa0644340 AT2G46920.3 47.848 395 158 9 355 710 457 842 2.79e-107 346
Msa0644340 AT2G46920.3 37.783 442 173 14 1 374 1 408 9.70e-69 242
Msa0644340 AT2G46920.1 47.848 395 158 9 355 710 457 842 2.79e-107 346
Msa0644340 AT2G46920.1 37.783 442 173 14 1 374 1 408 9.70e-69 242
Msa0644340 AT2G46920.2 47.848 395 158 9 355 710 457 842 2.79e-107 346
Msa0644340 AT2G46920.2 37.783 442 173 14 1 374 1 408 9.70e-69 242
Msa0644340 AT2G46920.4 47.848 395 158 9 355 710 457 842 2.79e-107 346
Msa0644340 AT2G46920.4 37.783 442 173 14 1 374 1 408 9.70e-69 242
Msa0644340 AT2G35350.1 53.438 320 118 7 408 710 473 778 6.89e-102 330
Msa0644340 AT2G35350.1 34.026 385 144 15 1 329 1 331 2.00e-39 156
Msa0644340 AT3G16560.1 38.511 309 143 4 408 703 206 480 2.55e-64 222
Msa0644340 AT3G16560.1 47.297 74 39 0 246 319 133 206 1.85e-12 70.9
Msa0644340 AT3G16560.4 38.511 309 143 4 408 703 206 480 2.55e-64 222
Msa0644340 AT3G16560.4 47.297 74 39 0 246 319 133 206 1.85e-12 70.9
Msa0644340 AT3G16560.2 38.511 309 143 4 408 703 206 480 2.55e-64 222
Msa0644340 AT3G16560.2 47.297 74 39 0 246 319 133 206 1.85e-12 70.9
Msa0644340 AT3G16560.3 36.655 281 131 4 408 675 206 452 2.40e-51 185
Msa0644340 AT3G16560.3 47.297 74 39 0 246 319 133 206 1.70e-12 70.9
Msa0644340 AT3G55050.1 31.541 279 145 6 434 705 121 360 9.53e-35 137
Msa0644340 AT3G55050.2 31.541 279 145 6 434 705 121 360 9.53e-35 137
Msa0644340 AT3G12620.2 30.208 288 159 5 425 707 111 361 1.69e-34 136
Msa0644340 AT3G12620.1 30.208 288 159 5 425 707 111 361 1.69e-34 136
Msa0644340 AT3G17090.1 30.545 275 148 4 434 703 122 358 9.60e-34 134
Msa0644340 AT4G38520.2 31.502 273 145 5 434 701 118 353 2.66e-33 133
Msa0644340 AT4G38520.1 31.502 273 145 5 434 701 118 353 2.66e-33 133
Msa0644340 AT3G51370.2 31.183 279 146 5 433 704 30 269 4.06e-33 129
Msa0644340 AT5G66080.2 30.612 294 158 6 416 704 1 253 5.25e-33 129
Msa0644340 AT3G51370.1 30.325 277 151 4 433 704 115 354 1.61e-32 130
Msa0644340 AT3G51370.3 30.325 277 151 4 433 704 115 354 1.61e-32 130
Msa0644340 AT5G66080.1 31.769 277 147 5 433 704 118 357 6.76e-32 128
Msa0644340 AT5G02760.1 32.000 275 143 5 434 703 109 344 3.39e-30 123
Msa0644340 AT4G33920.2 29.603 277 156 5 433 704 14 256 1.15e-29 120
Msa0644340 AT4G33920.1 29.603 277 156 5 433 704 97 339 2.88e-29 120
Msa0644340 AT5G06750.2 29.032 279 159 5 434 707 37 281 6.72e-29 118
Msa0644340 AT5G06750.3 29.032 279 159 5 434 707 119 363 1.42e-28 119
Msa0644340 AT5G06750.1 29.032 279 159 5 434 707 119 363 1.42e-28 119
Msa0644340 AT4G33920.3 31.076 251 134 5 459 704 10 226 7.81e-27 110
Msa0644340 AT3G17090.2 31.050 219 112 3 434 647 122 306 1.15e-24 105

Find 205 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCGGAACTTGCTTTAA+TGG 0.204565 4_3:-48182303 Msa0644340:CDS
ACGGTTACTGTTGTTGTTTC+CGG 0.205809 4_3:+48183636 None:intergenic
AGAAAGCCGAACCTGATTAT+TGG 0.247572 4_3:-48182199 Msa0644340:CDS
TTCCATCATTGTTATTTCTT+TGG 0.250349 4_3:-48180837 Msa0644340:CDS
TGGGAAAATTAGACAGGATT+TGG 0.255176 4_3:-48182174 Msa0644340:CDS
CAAGTGGAACAATGCACTTC+TGG 0.269345 4_3:-48181284 Msa0644340:CDS
TAAAGGATCCGTTATTAATA+TGG 0.281615 4_3:+48183600 None:intergenic
TTTGTTATGGGAAACAAAAT+AGG 0.293503 4_3:-48183679 Msa0644340:exon
TGTTAATCATTCAGATGTTT+TGG 0.294451 4_3:-48182396 Msa0644340:CDS
AGCTTCTCTCATAGTGGATT+AGG 0.296940 4_3:-48183148 Msa0644340:CDS
TTCGGTGGTGTTGGTGGTGG+TGG 0.306073 4_3:-48183316 Msa0644340:CDS
GCTTCTCTCATAGTGGATTA+GGG 0.330402 4_3:-48183147 Msa0644340:CDS
GGGAGATAGTAGAGCTGTTT+TGG 0.332474 4_3:-48182225 Msa0644340:CDS
TTTGTTCATAAGGAGCTTAA+AGG 0.334724 4_3:-48182743 Msa0644340:CDS
ATATGGATATGTCGTGTTTA+CGG 0.335656 4_3:+48183617 None:intergenic
AAAGGGTTGCTTTGGGATGA+TGG 0.341522 4_3:-48182725 Msa0644340:CDS
TTTGAGTAGTGAAGGTAGTT+TGG 0.342225 4_3:-48182936 Msa0644340:CDS
AGGAGCTTAAAGGGTTGCTT+TGG 0.344311 4_3:-48182733 Msa0644340:CDS
TATATTATGATGATGATATA+AGG 0.344834 4_3:+48180767 None:intergenic
TTTCTTTGGAAGGAAGGATA+TGG 0.347821 4_3:-48180823 Msa0644340:CDS
AATCCGGAACTTGCTTTAAT+GGG 0.351962 4_3:-48182302 Msa0644340:CDS
GGTGGTGGGTTTCCGAGTTC+CGG 0.357726 4_3:-48183301 Msa0644340:CDS
GCCGTTATCGACGGCGTTCA+TGG 0.360427 4_3:-48183443 Msa0644340:CDS
TGTTGAGCAGGGTCTCCTTC+AGG 0.362431 4_3:+48181085 None:intergenic
CAGAAGTGCATTGTTCCACT+TGG 0.376775 4_3:+48181285 None:intergenic
AAGGGTTGCTTTGGGATGAT+GGG 0.382657 4_3:-48182724 Msa0644340:CDS
ACCACGGCACAAGGATCATC+TGG 0.391223 4_3:+48181916 None:intergenic
TAGTTCTTGTTCCTAGTACT+TGG 0.392726 4_3:-48183762 Msa0644340:five_prime_UTR
GAAAATAAACCACGATCAAT+CGG 0.401422 4_3:+48183223 None:intergenic
CTCAAGGTTACTCGAGCATT+TGG 0.402324 4_3:-48181871 Msa0644340:CDS
CCCAATCCAATAATCAGGTT+CGG 0.403305 4_3:+48182193 None:intergenic
TTGTTCATAAGGAGCTTAAA+GGG 0.406793 4_3:-48182742 Msa0644340:CDS
TCGAAATTCCACAAGGTGAT+AGG 0.408336 4_3:-48180880 Msa0644340:CDS
CGTGGTCAATGATCGTGTTA+AGG 0.408967 4_3:-48181899 Msa0644340:CDS
GAAGATAAACGGGTCGGGTC+GGG 0.411687 4_3:+48183541 None:intergenic
GCCGAACCTGATTATTGGAT+TGG 0.412405 4_3:-48182194 Msa0644340:CDS
TATAATAATAAACAAAATCC+AGG 0.412450 4_3:+48180507 None:intergenic
AGGAGACCCTGCTCAACATT+TGG 0.412890 4_3:-48181080 Msa0644340:CDS
GTCGATGATGATGTTGTTGA+TGG 0.413519 4_3:-48182662 Msa0644340:CDS
CCACCGGAGTGGATTCTTGC+TGG 0.414509 4_3:-48182998 Msa0644340:CDS
GAACTTTGGAAGAAGATAAA+CGG 0.420518 4_3:+48183530 None:intergenic
GAAGACTCTGTCACGTGGAC+AGG 0.422051 4_3:-48183062 Msa0644340:CDS
TTCTATTGTTACTCCGATCA+AGG 0.425074 4_3:-48183038 Msa0644340:CDS
AAAGGAGATGGAAGTGTGAA+TGG 0.425538 4_3:-48182493 Msa0644340:CDS
AATCTGTGGTGTTTAAGATA+TGG 0.429109 4_3:+48181211 None:intergenic
GAACCCATTAAAGCAAGTTC+CGG 0.431709 4_3:+48182299 None:intergenic
GATGGAAGTGTGAATGGGAT+AGG 0.433001 4_3:-48182487 Msa0644340:CDS
TCCATGAACGCCGTCGATAA+CGG 0.433102 4_3:+48183442 None:intergenic
AAGCTCTTTCGCGAGCTTTG+AGG 0.433880 4_3:-48182373 Msa0644340:CDS
GGAGCTTAAAGGGTTGCTTT+GGG 0.434258 4_3:-48182732 Msa0644340:CDS
AACAAATAATCAGGTGCATC+AGG 0.434263 4_3:+48182782 None:intergenic
ACCAACACCACCGAAGTTAC+CGG 0.437250 4_3:+48183324 None:intergenic
ATTCTCCTCGTGCTTCTTCG+CGG 0.439380 4_3:+48182517 None:intergenic
CGGAGGCGCCGGAGATTGTT+CGG 0.441737 4_3:+48183485 None:intergenic
GACATGAACCCACGCTCAAT+CGG 0.443578 4_3:+48183250 None:intergenic
TCCGGTAACTTCGGTGGTGT+TGG 0.444115 4_3:-48183325 Msa0644340:CDS
CAATGGGCTCAAGGGAAAGC+AGG 0.451112 4_3:-48182881 Msa0644340:CDS
CAATGCATGATCTTGAATCA+TGG 0.452610 4_3:-48182133 Msa0644340:CDS
CCGAACCTGATTATTGGATT+GGG 0.454169 4_3:-48182193 Msa0644340:CDS
TTCCAAAGAAATAACAATGA+TGG 0.456946 4_3:+48180835 None:intergenic
AGATTTGACAACAAATAATC+AGG 0.457442 4_3:+48182773 None:intergenic
TTGGATTGGGAAAATTAGAC+AGG 0.458394 4_3:-48182180 Msa0644340:CDS
AGAGCTTGATAGAAGATTGA+AGG 0.459767 4_3:-48182456 Msa0644340:CDS
AAGATTTACCTCTTCAACAT+TGG 0.460239 4_3:+48182043 None:intergenic
GTGGTCAATGATCGTGTTAA+GGG 0.463001 4_3:-48181898 Msa0644340:CDS
TGGTGCCGGATTTCTCAAAC+AGG 0.467257 4_3:-48181851 Msa0644340:intron
TATCTTAAACACCACAGATT+AGG 0.469227 4_3:-48181208 Msa0644340:CDS
AAACTGATCAACGCCGGTGC+CGG 0.470846 4_3:+48183177 None:intergenic
AACTTTGGAAGAAGATAAAC+GGG 0.472058 4_3:+48183531 None:intergenic
TGTTTCTGAGGAACATGGTT+GGG 0.473614 4_3:-48182837 Msa0644340:CDS
TAAGATGGTGATGGAGAATC+CGG 0.475384 4_3:-48182318 Msa0644340:CDS
TTTGGAAGATGATGATTCGT+TGG 0.476363 4_3:-48182918 Msa0644340:CDS
TATGGTTGGCAAGTAATGTA+TGG 0.477336 4_3:+48181229 None:intergenic
ATAACGGATCCTTTAGATGA+AGG 0.479407 4_3:-48183592 Msa0644340:CDS
TTCTTGTAATGTTGATGAAA+GGG 0.479413 4_3:-48182271 Msa0644340:CDS
TTATGATGATGATATAAGGT+TGG 0.479889 4_3:+48180771 None:intergenic
TCGGTGGTGTTGGTGGTGGT+GGG 0.481492 4_3:-48183315 Msa0644340:CDS
TCTTCGACCAAATGTTGAGC+AGG 0.483562 4_3:+48181073 None:intergenic
TGACCAAGTCCTTCATCTAA+AGG 0.485372 4_3:+48183583 None:intergenic
GGATTTGGAGAGGATTAATG+AGG 0.485566 4_3:-48182159 Msa0644340:CDS
ATCATTGTTATTTCTTTGGA+AGG 0.487899 4_3:-48180833 Msa0644340:CDS
AAACAAAATAGGAAAATTAA+CGG 0.488093 4_3:-48183668 Msa0644340:CDS
AACTTCGGTGGTGTTGGTGG+TGG 0.488939 4_3:-48183319 Msa0644340:CDS
CAGAGAAGCTTCTCTCATAG+TGG 0.489328 4_3:-48183154 Msa0644340:CDS
TTGAAAGATTAGTGGGAACT+AGG 0.489496 4_3:+48183850 None:intergenic
TTGTTATTTCTTTGGAAGGA+AGG 0.491846 4_3:-48180829 Msa0644340:CDS
AACATTGGTACTATGATCCT+TGG 0.492918 4_3:+48182058 None:intergenic
TTCATGGATTTATACTCCTA+CGG 0.494403 4_3:-48183427 Msa0644340:CDS
TTGGGATGATGGGGTTTCAT+CGG 0.500864 4_3:-48182714 Msa0644340:CDS
AGAAGATAAACGGGTCGGGT+CGG 0.501483 4_3:+48183540 None:intergenic
AAATTAACGGTATGTTTCAC+CGG 0.503917 4_3:-48183655 Msa0644340:CDS
ACGAACCTGTTTGAGAAATC+CGG 0.503935 4_3:+48181846 None:intergenic
ATTGAGCGTGGGTTCATGTC+CGG 0.505314 4_3:-48183247 Msa0644340:CDS
AGGGTTGCTTTGGGATGATG+GGG 0.507169 4_3:-48182723 Msa0644340:CDS
CGTTGAAGTGTTGGCGCTGA+CGG 0.507965 4_3:+48183465 None:intergenic
AGTTTGAATTTGAGTAGTGA+AGG 0.511209 4_3:-48182944 Msa0644340:CDS
ACAACTTTCCGAACAATCTC+CGG 0.511363 4_3:-48183493 Msa0644340:CDS
CGCTCTAACGGACCGGAACT+CGG 0.513564 4_3:+48183289 None:intergenic
TCCGTTGCAACCGATTCCGA+AGG 0.513883 4_3:-48183353 Msa0644340:CDS
TGATGGTGTTGTTGTGAAAG+AGG 0.514346 4_3:-48182645 Msa0644340:CDS
TTAGAGCGTGGGTTTATGTC+CGG 0.514791 4_3:-48183274 Msa0644340:CDS
CGACATATCCATATTAATAA+CGG 0.515144 4_3:-48183608 Msa0644340:CDS
AAGGAGATGGAAGTGTGAAT+GGG 0.515548 4_3:-48182492 Msa0644340:CDS
GTTCCGGTCCGTTAGAGCGT+GGG 0.516393 4_3:-48183285 Msa0644340:CDS
TCGTTTATATTTAAATTAGG+AGG 0.518447 4_3:-48180577 Msa0644340:three_prime_UTR
TACTCCGATCAAGGGTGTGC+CGG 0.519255 4_3:-48183029 Msa0644340:CDS
GGTAACTTCGGTGGTGTTGG+TGG 0.519950 4_3:-48183322 Msa0644340:CDS
TTCACCGGCACACCCTTGAT+CGG 0.520030 4_3:+48183025 None:intergenic
CACTCCGGTGGCTCCTTCAC+CGG 0.520356 4_3:+48183010 None:intergenic
GACATAAACCCACGCTCTAA+CGG 0.522963 4_3:+48183277 None:intergenic
GATCAAGGGTGTGCCGGTGA+AGG 0.523754 4_3:-48183023 Msa0644340:CDS
TTGGAAGATGATGATTCGTT+GGG 0.524167 4_3:-48182917 Msa0644340:CDS
CGATAACGGCGTTGAAGTGT+TGG 0.524171 4_3:+48183456 None:intergenic
ATGGAAGTGTGAATGGGATA+GGG 0.524574 4_3:-48182486 Msa0644340:CDS
GGTTTCTTCAGAGTGAACTT+TGG 0.525914 4_3:+48183516 None:intergenic
TGGGAAGTCAGAATCTTCAA+TGG 0.526449 4_3:-48182898 Msa0644340:CDS
ATGAATAGAAACCAAGTACT+AGG 0.526919 4_3:+48183751 None:intergenic
AGAAGCACGAGGAGAATCAA+AGG 0.529931 4_3:-48182511 Msa0644340:CDS
TTTCGCGCTGCAAAGAAAGC+TGG 0.530460 4_3:-48181043 Msa0644340:intron
TGAAGTGTTGGCGCTGACGG+AGG 0.531029 4_3:+48183468 None:intergenic
GATGTTTATGTGATGAATGT+GGG 0.536014 4_3:-48182245 Msa0644340:CDS
CAATGTGTTGATCAAGAGAA+AGG 0.538305 4_3:-48182599 Msa0644340:CDS
ATGTCCGGTCCGATTGAGCG+TGG 0.539933 4_3:-48183259 Msa0644340:CDS
AGAAGTGCATTGTTCCACTT+GGG 0.541493 4_3:+48181286 None:intergenic
GTTGGCGCTGACGGAGGCGC+CGG 0.543294 4_3:+48183474 None:intergenic
CGATCATTGACCACGGCACA+AGG 0.546342 4_3:+48181907 None:intergenic
AGATGTTTATGTGATGAATG+TGG 0.546822 4_3:-48182246 Msa0644340:CDS
TCTTGATGTTGCGGATAAGA+TGG 0.547525 4_3:-48182333 Msa0644340:CDS
GGGAAGTCAGAATCTTCAAT+GGG 0.550933 4_3:-48182897 Msa0644340:CDS
GCCTTCGGAATCGGTTGCAA+CGG 0.551003 4_3:+48183352 None:intergenic
GTTACTCGAGCATTTGGTGC+CGG 0.551671 4_3:-48181865 Msa0644340:CDS
CACTTCAACGCCGTTATCGA+CGG 0.551722 4_3:-48183452 Msa0644340:CDS
AGTTCCGGTCCGTTAGAGCG+TGG 0.554284 4_3:-48183286 Msa0644340:CDS
CAGAATCTTCAATGGGCTCA+AGG 0.556444 4_3:-48182890 Msa0644340:CDS
TTCATCACATTACAACCTGA+AGG 0.557463 4_3:-48181100 Msa0644340:CDS
GAGAGAGATTGTGAAGTGAA+TGG 0.558486 4_3:+48183885 None:intergenic
TGGAAGAAGATAAACGGGTC+GGG 0.560735 4_3:+48183536 None:intergenic
TCTATTGTTACTCCGATCAA+GGG 0.565520 4_3:-48183037 Msa0644340:CDS
GTTCTTGTAATGTTGATGAA+AGG 0.566141 4_3:-48182272 Msa0644340:CDS
GATCCTTTAGATGAAGGACT+TGG 0.568383 4_3:-48183586 Msa0644340:CDS
GATAAAGATAGCTCCGGCAC+CGG 0.569242 4_3:-48183190 Msa0644340:CDS
TGTCCGGTCCGATTGAGCGT+GGG 0.569809 4_3:-48183258 Msa0644340:CDS
GGAGATTGTTCGGAAAGTTG+TGG 0.572383 4_3:+48183495 None:intergenic
GTTATTGTTTGCAGCCCAAG+TGG 0.572427 4_3:-48181300 Msa0644340:intron
AATGGGCTCAAGGGAAAGCA+GGG 0.572972 4_3:-48182880 Msa0644340:CDS
TTTCGGTTCGACCGAGATCG+AGG 0.574008 4_3:-48183123 Msa0644340:CDS
AAATTAGACAGGATTTGGAG+AGG 0.574734 4_3:-48182169 Msa0644340:CDS
AAACCCACGCTCTAACGGAC+CGG 0.575313 4_3:+48183282 None:intergenic
TGTGCCGGTGAAGGAGCCAC+CGG 0.575890 4_3:-48183014 Msa0644340:CDS
TGTTCATGTTGTTGTTTCTG+AGG 0.576807 4_3:-48182849 Msa0644340:CDS
AATGCATGATCTTGAATCAT+GGG 0.577709 4_3:-48182132 Msa0644340:CDS
AGAATCTTCAATGGGCTCAA+GGG 0.581264 4_3:-48182889 Msa0644340:CDS
GTTGTTGTTTCTGAGGAACA+TGG 0.582318 4_3:-48182842 Msa0644340:CDS
CCGGAGCTATCTTTATCCAT+CGG 0.582787 4_3:+48183196 None:intergenic
GAACCCACGCTCAATCGGAC+CGG 0.583850 4_3:+48183255 None:intergenic
AGCTGAAAAGCACTTAAACT+AGG 0.588518 4_3:+48182083 None:intergenic
TTGTTTCTGAGGAACATGGT+TGG 0.588748 4_3:-48182838 Msa0644340:CDS
GGAGTAATTATGTAAATCAG+TGG 0.593753 4_3:-48180556 Msa0644340:three_prime_UTR
ACGAGGAGAATCAAAGGAGA+TGG 0.596338 4_3:-48182505 Msa0644340:CDS
GAGCAACTAAGTGGTGGTGA+TGG 0.596834 4_3:-48182434 Msa0644340:CDS
ATGTAAATCAGTGGATAGTG+AGG 0.597119 4_3:-48180547 Msa0644340:three_prime_UTR
TCATAGTACCAATGTTGAAG+AGG 0.597252 4_3:-48182051 Msa0644340:intron
ATGTCCGGTCCGATTGATCG+TGG 0.599187 4_3:-48183232 Msa0644340:CDS
AACATCAAGATAAGACTCCT+CGG 0.601800 4_3:+48182346 None:intergenic
TCTTGTAATGTTGATGAAAG+GGG 0.604649 4_3:-48182270 Msa0644340:CDS
TTGGAAGAAGATAAACGGGT+CGG 0.606093 4_3:+48183535 None:intergenic
CTTCGACCAAATGTTGAGCA+GGG 0.606676 4_3:+48181074 None:intergenic
ATAATAATAAACAAAATCCA+GGG 0.607632 4_3:+48180508 None:intergenic
GATTGTAAAGTTGTAAACCC+TGG 0.609142 4_3:-48180525 Msa0644340:three_prime_UTR
TCGACACTCGTGAATAGCGA+GGG 0.610488 4_3:+48180682 None:intergenic
AAGACTCTGTCACGTGGACA+GGG 0.611869 4_3:-48183061 Msa0644340:CDS
GCTATCTTTATCCATCGGAC+CGG 0.612481 4_3:+48183201 None:intergenic
TCGTGTTAAGGGATCTCTCA+AGG 0.616145 4_3:-48181887 Msa0644340:CDS
CAACTAAGTGGTGGTGATGG+TGG 0.617990 4_3:-48182431 Msa0644340:CDS
CCCAGATGATCCTTGTGCCG+TGG 0.618955 4_3:-48181917 Msa0644340:CDS
CCACGGCACAAGGATCATCT+GGG 0.623926 4_3:+48181917 None:intergenic
TGTGGTGTTTAAGATATGGT+TGG 0.627286 4_3:+48181215 None:intergenic
GGAGTCTTATCTTGATGTTG+CGG 0.628468 4_3:-48182342 Msa0644340:CDS
ATGAGATATATGCTGCCGAA+GGG 0.630709 4_3:-48180729 Msa0644340:three_prime_UTR
TATGAGATATATGCTGCCGA+AGG 0.630877 4_3:-48180730 Msa0644340:three_prime_UTR
TAACGCCGCCTATCACCTTG+TGG 0.632464 4_3:+48180872 None:intergenic
CAAGTATTGATAGAGTCCGT+CGG 0.633991 4_3:+48181156 None:intergenic
CCGATGGATAAAGATAGCTC+CGG 0.640588 4_3:-48183196 Msa0644340:CDS
TAAACCACGATCAATCGGAC+CGG 0.640729 4_3:+48183228 None:intergenic
TGTTGCGGATAAGATGGTGA+TGG 0.644164 4_3:-48182327 Msa0644340:CDS
CTCGACACTCGTGAATAGCG+AGG 0.645488 4_3:+48180681 None:intergenic
CCAGCAAGAATCCACTCCGG+TGG 0.655460 4_3:+48182998 None:intergenic
GCGAGCTTTGAGGAAAACCG+AGG 0.665283 4_3:-48182363 Msa0644340:CDS
AGATGCCGCGAAGAAGCACG+AGG 0.671726 4_3:-48182522 Msa0644340:CDS
CGGTGAAGGAGCCACCGGAG+TGG 0.673007 4_3:-48183009 Msa0644340:CDS
AAATTCCACAAGGTGATAGG+CGG 0.673197 4_3:-48180877 Msa0644340:CDS
AGATTGAAGGAGCAACTAAG+TGG 0.673447 4_3:-48182443 Msa0644340:CDS
TTGAAGGAGCAACTAAGTGG+TGG 0.673586 4_3:-48182440 Msa0644340:CDS
AGCTCTATCAAAACAACCGT+AGG 0.682369 4_3:+48183411 None:intergenic
ATTTCGAAGACTCTGTCACG+TGG 0.685701 4_3:-48183067 Msa0644340:CDS
TCACCAGCAAGAATCCACTC+CGG 0.688259 4_3:+48182995 None:intergenic
TCTCTGAAACTGATCAACGC+CGG 0.696982 4_3:+48183171 None:intergenic
CGACACTCGTGAATAGCGAG+GGG 0.698659 4_3:+48180683 None:intergenic
ATGTTTAGAATAGACTACGT+AGG 0.701650 4_3:-48181259 Msa0644340:CDS
TCATCATCGACTGAATCGCG+TGG 0.705816 4_3:+48182674 None:intergenic
CAATGATGGAAACATCGTCG+TGG 0.706639 4_3:+48180849 None:intergenic
CTTAACACGATCATTGACCA+CGG 0.708721 4_3:+48181900 None:intergenic
ATGGGCTCAAGGGAAAGCAG+GGG 0.712223 4_3:-48182879 Msa0644340:CDS
GAATTGCTCGAAATTCCACA+AGG 0.753407 4_3:-48180887 Msa0644340:CDS
GACACTCGTGAATAGCGAGG+GGG 0.766486 4_3:+48180684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTAATTTTTGTTAT+GGG - chr4_3:48180636-48180655 Msa0644340:three_prime_UTR 10.0%
!!! TTTTCGTTTATATTTAAATT+AGG - chr4_3:48183747-48183766 Msa0644340:five_prime_UTR 10.0%
!!! GTGTTTTTTAATTTTTGTTA+TGG - chr4_3:48180635-48180654 Msa0644340:three_prime_UTR 15.0%
!! AAACAAAATAGGAAAATTAA+CGG - chr4_3:48180659-48180678 Msa0644340:three_prime_UTR 15.0%
!!! TATATTATGATGATGATATA+AGG + chr4_3:48183563-48183582 None:intergenic 15.0%
!!! TTTTTGCTTATATTTAAGTT+CGG + chr4_3:48183685-48183704 None:intergenic 15.0%
!! ATAATAATAAACAAAATCCA+GGG + chr4_3:48183822-48183841 None:intergenic 15.0%
!! TATAATAATAAACAAAATCC+AGG + chr4_3:48183823-48183842 None:intergenic 15.0%
!!! TCTTTATAGTTTTGTTCATA+AGG - chr4_3:48181574-48181593 Msa0644340:intron 20.0%
!! ATTTCAAAGAAAAAAAAGAG+AGG - chr4_3:48181758-48181777 Msa0644340:intron 20.0%
!! AAGTGTTTGATTATAATTTC+AGG - chr4_3:48182362-48182381 Msa0644340:CDS 20.0%
!! TTATTAATCTTCTACAAGAA+TGG + chr4_3:48182763-48182782 None:intergenic 20.0%
!!! GAATCAAAAATTTGTCTTTT+TGG + chr4_3:48183145-48183164 None:intergenic 20.0%
!! TATTCTTCTTAATCTTAATC+AGG - chr4_3:48183404-48183423 Msa0644340:CDS 20.0%
!! TCGTTTATATTTAAATTAGG+AGG - chr4_3:48183750-48183769 Msa0644340:five_prime_UTR 20.0%
!!! ATGAAAAGATTTTTAAGGAA+TGG + chr4_3:48183885-48183904 None:intergenic 20.0%
!! TTTTGTGTTGAAAGATTAGT+GGG + chr4_3:48180487-48180506 None:intergenic 25.0%
!!! ATAACTCGTTTTTCATGTTT+TGG + chr4_3:48180510-48180529 None:intergenic 25.0%
!! GAACTATGAAACAAGTTTTA+AGG + chr4_3:48180551-48180570 None:intergenic 25.0%
!! TTTGTTATGGGAAACAAAAT+AGG - chr4_3:48180648-48180667 Msa0644340:three_prime_UTR 25.0%
! CGACATATCCATATTAATAA+CGG - chr4_3:48180719-48180738 Msa0644340:three_prime_UTR 25.0%
! TAAAGGATCCGTTATTAATA+TGG + chr4_3:48180730-48180749 None:intergenic 25.0%
! AGATTTGACAACAAATAATC+AGG + chr4_3:48181557-48181576 None:intergenic 25.0%
!! TGTTAATCATTCAGATGTTT+TGG - chr4_3:48181931-48181950 Msa0644340:CDS 25.0%
!! TTCTTGTAATGTTGATGAAA+GGG - chr4_3:48182056-48182075 Msa0644340:CDS 25.0%
! CCATTAATTAAGAAACTCAA+CGG - chr4_3:48182544-48182563 Msa0644340:CDS 25.0%
!! AATCATATATCCTATCCTTT+TGG + chr4_3:48182667-48182686 None:intergenic 25.0%
! TTCTTTCCTTTCACAAAAAA+TGG - chr4_3:48182806-48182825 Msa0644340:CDS 25.0%
!!! AATGTTCCATTTTTTGTGAA+AGG + chr4_3:48182815-48182834 None:intergenic 25.0%
! TGAAAATCCATTAAAGAGTT+AGG + chr4_3:48182842-48182861 None:intergenic 25.0%
! TAACATTTGTAGTTAGAGTT+AGG + chr4_3:48183011-48183030 None:intergenic 25.0%
! GCAATTGTGAAATCTTAAAA+TGG + chr4_3:48183321-48183340 None:intergenic 25.0%
! TTCTTAATCTTAATCAGGTT+TGG - chr4_3:48183409-48183428 Msa0644340:CDS 25.0%
!!! TTCCATCATTGTTATTTCTT+TGG - chr4_3:48183490-48183509 Msa0644340:CDS 25.0%
! TTCCAAAGAAATAACAATGA+TGG + chr4_3:48183495-48183514 None:intergenic 25.0%
!!! ATCATTGTTATTTCTTTGGA+AGG - chr4_3:48183494-48183513 Msa0644340:CDS 25.0%
!! TTATGATGATGATATAAGGT+TGG + chr4_3:48183559-48183578 None:intergenic 25.0%
! GTTTTGTGTTGAAAGATTAG+TGG + chr4_3:48180488-48180507 None:intergenic 30.0%
ATGAATAGAAACCAAGTACT+AGG + chr4_3:48180579-48180598 None:intergenic 30.0%
AAATTAACGGTATGTTTCAC+CGG - chr4_3:48180672-48180691 Msa0644340:three_prime_UTR 30.0%
ATATGGATATGTCGTGTTTA+CGG + chr4_3:48180713-48180732 None:intergenic 30.0%
! AACTTTGGAAGAAGATAAAC+GGG + chr4_3:48180799-48180818 None:intergenic 30.0%
! GAACTTTGGAAGAAGATAAA+CGG + chr4_3:48180800-48180819 None:intergenic 30.0%
TTCATGGATTTATACTCCTA+CGG - chr4_3:48180900-48180919 Msa0644340:CDS 30.0%
GAAAATAAACCACGATCAAT+CGG + chr4_3:48181107-48181126 None:intergenic 30.0%
!! ATTGATCGTGGTTTATTTTC+CGG - chr4_3:48181107-48181126 Msa0644340:CDS 30.0%
AGTTTGAATTTGAGTAGTGA+AGG - chr4_3:48181383-48181402 Msa0644340:intron 30.0%
! GTTTTTGTTGGGATTTATGA+TGG - chr4_3:48181512-48181531 Msa0644340:intron 30.0%
!!! GGGATTTATGATGGTTTTAA+TGG - chr4_3:48181521-48181540 Msa0644340:intron 30.0%
TTTGTTCATAAGGAGCTTAA+AGG - chr4_3:48181584-48181603 Msa0644340:intron 30.0%
TTGTTCATAAGGAGCTTAAA+GGG - chr4_3:48181585-48181604 Msa0644340:intron 30.0%
! GTTCTTGTAATGTTGATGAA+AGG - chr4_3:48182055-48182074 Msa0644340:CDS 30.0%
! TCTTGTAATGTTGATGAAAG+GGG - chr4_3:48182057-48182076 Msa0644340:CDS 30.0%
AGATGTTTATGTGATGAATG+TGG - chr4_3:48182081-48182100 Msa0644340:CDS 30.0%
GATGTTTATGTGATGAATGT+GGG - chr4_3:48182082-48182101 Msa0644340:CDS 30.0%
! ATTTTCCCAATCCAATAATC+AGG + chr4_3:48182142-48182161 None:intergenic 30.0%
AATGCATGATCTTGAATCAT+GGG - chr4_3:48182195-48182214 Msa0644340:CDS 30.0%
AAGATTTACCTCTTCAACAT+TGG + chr4_3:48182287-48182306 None:intergenic 30.0%
CCGTTGAGTTTCTTAATTAA+TGG + chr4_3:48182547-48182566 None:intergenic 30.0%
TAATTAAGAAACTCAACGGA+AGG - chr4_3:48182548-48182567 Msa0644340:CDS 30.0%
! ATATCCTATCCTTTTGGATT+TGG + chr4_3:48182661-48182680 None:intergenic 30.0%
! ATTTGTTTCTCTTGTCACAA+AGG - chr4_3:48182720-48182739 Msa0644340:CDS 30.0%
ATTGACACCTAACTCTTTAA+TGG - chr4_3:48182832-48182851 Msa0644340:CDS 30.0%
TGTCATATTAATGCTAAGCA+TGG - chr4_3:48182866-48182885 Msa0644340:CDS 30.0%
AACAGAAAATGTTTCACACT+TGG - chr4_3:48182936-48182955 Msa0644340:CDS 30.0%
! ATGTTTAGAATAGACTACGT+AGG - chr4_3:48183068-48183087 Msa0644340:CDS 30.0%
! AATCTGTGGTGTTTAAGATA+TGG + chr4_3:48183119-48183138 None:intergenic 30.0%
TATCTTAAACACCACAGATT+AGG - chr4_3:48183119-48183138 Msa0644340:CDS 30.0%
!! TTGTTATTTCTTTGGAAGGA+AGG - chr4_3:48183498-48183517 Msa0644340:CDS 30.0%
GGAGTAATTATGTAAATCAG+TGG - chr4_3:48183771-48183790 Msa0644340:five_prime_UTR 30.0%
TTGAAAGATTAGTGGGAACT+AGG + chr4_3:48180480-48180499 None:intergenic 35.0%
TAGTTCTTGTTCCTAGTACT+TGG - chr4_3:48180565-48180584 Msa0644340:three_prime_UTR 35.0%
!! ATAACGGATCCTTTAGATGA+AGG - chr4_3:48180735-48180754 Msa0644340:three_prime_UTR 35.0%
!!! TCATAGTGGATTAGGGTTTT+CGG - chr4_3:48181187-48181206 Msa0644340:CDS 35.0%
! CATTTTTCTTTCCTCGATCT+CGG + chr4_3:48181218-48181237 None:intergenic 35.0%
TTCTATTGTTACTCCGATCA+AGG - chr4_3:48181289-48181308 Msa0644340:CDS 35.0%
TCTATTGTTACTCCGATCAA+GGG - chr4_3:48181290-48181309 Msa0644340:intron 35.0%
TTTGAGTAGTGAAGGTAGTT+TGG - chr4_3:48181391-48181410 Msa0644340:intron 35.0%
TTTGGAAGATGATGATTCGT+TGG - chr4_3:48181409-48181428 Msa0644340:intron 35.0%
TTGGAAGATGATGATTCGTT+GGG - chr4_3:48181410-48181429 Msa0644340:intron 35.0%
!! TGTTCATGTTGTTGTTTCTG+AGG - chr4_3:48181478-48181497 Msa0644340:intron 35.0%
! AACATGGTTGGGTTTTTGTT+GGG - chr4_3:48181501-48181520 Msa0644340:intron 35.0%
AACAAATAATCAGGTGCATC+AGG + chr4_3:48181548-48181567 None:intergenic 35.0%
! CAATGTGTTGATCAAGAGAA+AGG - chr4_3:48181728-48181747 Msa0644340:intron 35.0%
AGAGCTTGATAGAAGATTGA+AGG - chr4_3:48181871-48181890 Msa0644340:CDS 35.0%
AACATCAAGATAAGACTCCT+CGG + chr4_3:48181984-48182003 None:intergenic 35.0%
! AATCCGGAACTTGCTTTAAT+GGG - chr4_3:48182025-48182044 Msa0644340:intron 35.0%
! TTGGATTGGGAAAATTAGAC+AGG - chr4_3:48182147-48182166 Msa0644340:CDS 35.0%
TGGGAAAATTAGACAGGATT+TGG - chr4_3:48182153-48182172 Msa0644340:CDS 35.0%
AAATTAGACAGGATTTGGAG+AGG - chr4_3:48182158-48182177 Msa0644340:CDS 35.0%
CAATGCATGATCTTGAATCA+TGG - chr4_3:48182194-48182213 Msa0644340:CDS 35.0%
!! AGCTGAAAAGCACTTAAACT+AGG + chr4_3:48182247-48182266 None:intergenic 35.0%
AACATTGGTACTATGATCCT+TGG + chr4_3:48182272-48182291 None:intergenic 35.0%
TCATAGTACCAATGTTGAAG+AGG - chr4_3:48182276-48182295 Msa0644340:CDS 35.0%
CCAAAACAGAAGTCTAAAAG+AGG + chr4_3:48182312-48182331 None:intergenic 35.0%
! CCTCTTTTAGACTTCTGTTT+TGG - chr4_3:48182309-48182328 Msa0644340:CDS 35.0%
! CTATCCTTTTGGATTTGGAT+TGG + chr4_3:48182656-48182675 None:intergenic 35.0%
CAATCCAAATCCAAAAGGAT+AGG - chr4_3:48182654-48182673 Msa0644340:CDS 35.0%
TGTGACAAGAGAAACAAATG+TGG + chr4_3:48182719-48182738 None:intergenic 35.0%
CACAAAGGAATACATATGCT+AGG - chr4_3:48182735-48182754 Msa0644340:CDS 35.0%
TATGGTTGGCAAGTAATGTA+TGG + chr4_3:48183101-48183120 None:intergenic 35.0%
! TGTGGTGTTTAAGATATGGT+TGG + chr4_3:48183115-48183134 None:intergenic 35.0%
!! TTTTTGATTCTATGCTCCGA+CGG - chr4_3:48183155-48183174 Msa0644340:CDS 35.0%
TTCATCACATTACAACCTGA+AGG - chr4_3:48183227-48183246 Msa0644340:CDS 35.0%
! TTTCTTTGGAAGGAAGGATA+TGG - chr4_3:48183504-48183523 Msa0644340:CDS 35.0%
ATGTAAATCAGTGGATAGTG+AGG - chr4_3:48183780-48183799 Msa0644340:five_prime_UTR 35.0%
GATTGTAAAGTTGTAAACCC+TGG - chr4_3:48183802-48183821 Msa0644340:five_prime_UTR 35.0%
!! ACGGTTACTGTTGTTGTTTC+CGG + chr4_3:48180694-48180713 None:intergenic 40.0%
GATCCTTTAGATGAAGGACT+TGG - chr4_3:48180741-48180760 Msa0644340:three_prime_UTR 40.0%
TGACCAAGTCCTTCATCTAA+AGG + chr4_3:48180747-48180766 None:intergenic 40.0%
TTGGAAGAAGATAAACGGGT+CGG + chr4_3:48180795-48180814 None:intergenic 40.0%
GGTTTCTTCAGAGTGAACTT+TGG + chr4_3:48180814-48180833 None:intergenic 40.0%
ACAACTTTCCGAACAATCTC+CGG - chr4_3:48180834-48180853 Msa0644340:CDS 40.0%
AGCTCTATCAAAACAACCGT+AGG + chr4_3:48180919-48180938 None:intergenic 40.0%
AAGTTACCGGAAAAAGCCTT+CGG + chr4_3:48180993-48181012 None:intergenic 40.0%
!! AAGGCTTTTTCCGGTAACTT+CGG - chr4_3:48180993-48181012 Msa0644340:intron 40.0%
!! AGCTTCTCTCATAGTGGATT+AGG - chr4_3:48181179-48181198 Msa0644340:CDS 40.0%
!! GCTTCTCTCATAGTGGATTA+GGG - chr4_3:48181180-48181199 Msa0644340:CDS 40.0%
CGAGATCGAGGAAAGAAAAA+TGG - chr4_3:48181216-48181235 Msa0644340:CDS 40.0%
TGGGAAGTCAGAATCTTCAA+TGG - chr4_3:48181429-48181448 Msa0644340:intron 40.0%
GGGAAGTCAGAATCTTCAAT+GGG - chr4_3:48181430-48181449 Msa0644340:intron 40.0%
! AGAATCTTCAATGGGCTCAA+GGG - chr4_3:48181438-48181457 Msa0644340:intron 40.0%
!! GTTGTTGTTTCTGAGGAACA+TGG - chr4_3:48181485-48181504 Msa0644340:intron 40.0%
!! TTGTTTCTGAGGAACATGGT+TGG - chr4_3:48181489-48181508 Msa0644340:intron 40.0%
! TGTTTCTGAGGAACATGGTT+GGG - chr4_3:48181490-48181509 Msa0644340:intron 40.0%
! GAACATGGTTGGGTTTTTGT+TGG - chr4_3:48181500-48181519 Msa0644340:intron 40.0%
! GTCGATGATGATGTTGTTGA+TGG - chr4_3:48181665-48181684 Msa0644340:intron 40.0%
!! TGATGGTGTTGTTGTGAAAG+AGG - chr4_3:48181682-48181701 Msa0644340:intron 40.0%
! AAAGGAGATGGAAGTGTGAA+TGG - chr4_3:48181834-48181853 Msa0644340:intron 40.0%
! AAGGAGATGGAAGTGTGAAT+GGG - chr4_3:48181835-48181854 Msa0644340:intron 40.0%
! ATGGAAGTGTGAATGGGATA+GGG - chr4_3:48181841-48181860 Msa0644340:intron 40.0%
AGATTGAAGGAGCAACTAAG+TGG - chr4_3:48181884-48181903 Msa0644340:CDS 40.0%
! GGAGTCTTATCTTGATGTTG+CGG - chr4_3:48181985-48182004 Msa0644340:intron 40.0%
TCTTGATGTTGCGGATAAGA+TGG - chr4_3:48181994-48182013 Msa0644340:intron 40.0%
!! TAAGATGGTGATGGAGAATC+CGG - chr4_3:48182009-48182028 Msa0644340:intron 40.0%
! GAATCCGGAACTTGCTTTAA+TGG - chr4_3:48182024-48182043 Msa0644340:intron 40.0%
GAACCCATTAAAGCAAGTTC+CGG + chr4_3:48182031-48182050 None:intergenic 40.0%
AGAAAGCCGAACCTGATTAT+TGG - chr4_3:48182128-48182147 Msa0644340:CDS 40.0%
CCCAATCCAATAATCAGGTT+CGG + chr4_3:48182137-48182156 None:intergenic 40.0%
CCGAACCTGATTATTGGATT+GGG - chr4_3:48182134-48182153 Msa0644340:CDS 40.0%
GGATTTGGAGAGGATTAATG+AGG - chr4_3:48182168-48182187 Msa0644340:CDS 40.0%
! AAGTGCTTTTCAGCTTACCA+AGG - chr4_3:48182252-48182271 Msa0644340:CDS 40.0%
CTTAACACGATCATTGACCA+CGG + chr4_3:48182430-48182449 None:intergenic 40.0%
GTGGTCAATGATCGTGTTAA+GGG - chr4_3:48182429-48182448 Msa0644340:CDS 40.0%
ACGAACCTGTTTGAGAAATC+CGG + chr4_3:48182484-48182503 None:intergenic 40.0%
TAAGAAACTCAACGGAAGGA+TGG - chr4_3:48182552-48182571 Msa0644340:CDS 40.0%
! GGATGGATTTAGATTGCTTG+CGG - chr4_3:48182569-48182588 Msa0644340:CDS 40.0%
!!! TTTTGGATTTGGATTGGCTG+CGG + chr4_3:48182650-48182669 None:intergenic 40.0%
! AGAAGTGCATTGTTCCACTT+GGG + chr4_3:48183044-48183063 None:intergenic 40.0%
!! TGTCTTTTTGGCCTAATCTG+TGG + chr4_3:48183133-48183152 None:intergenic 40.0%
!! CAAGTATTGATAGAGTCCGT+CGG + chr4_3:48183174-48183193 None:intergenic 40.0%
GAATTGCTCGAAATTCCACA+AGG - chr4_3:48183440-48183459 Msa0644340:CDS 40.0%
TCGAAATTCCACAAGGTGAT+AGG - chr4_3:48183447-48183466 Msa0644340:CDS 40.0%
AAATTCCACAAGGTGATAGG+CGG - chr4_3:48183450-48183469 Msa0644340:CDS 40.0%
TATGAGATATATGCTGCCGA+AGG - chr4_3:48183597-48183616 Msa0644340:CDS 40.0%
ATGAGATATATGCTGCCGAA+GGG - chr4_3:48183598-48183617 Msa0644340:CDS 40.0%
AAAAAGGATGCATGACCCTT+CGG + chr4_3:48183616-48183635 None:intergenic 40.0%
TGGAAGAAGATAAACGGGTC+GGG + chr4_3:48180794-48180813 None:intergenic 45.0%
GGAGATTGTTCGGAAAGTTG+TGG + chr4_3:48180835-48180854 None:intergenic 45.0%
GACATAAACCCACGCTCTAA+CGG + chr4_3:48181053-48181072 None:intergenic 45.0%
TTAGAGCGTGGGTTTATGTC+CGG - chr4_3:48181053-48181072 Msa0644340:CDS 45.0%
TAAACCACGATCAATCGGAC+CGG + chr4_3:48181102-48181121 None:intergenic 45.0%
!! TGGTTTATTTTCCGGTCCGA+TGG - chr4_3:48181115-48181134 Msa0644340:CDS 45.0%
GCTATCTTTATCCATCGGAC+CGG + chr4_3:48181129-48181148 None:intergenic 45.0%
CCGGAGCTATCTTTATCCAT+CGG + chr4_3:48181134-48181153 None:intergenic 45.0%
CCGATGGATAAAGATAGCTC+CGG - chr4_3:48181131-48181150 Msa0644340:CDS 45.0%
!! TCTCTGAAACTGATCAACGC+CGG + chr4_3:48181159-48181178 None:intergenic 45.0%
CAGAGAAGCTTCTCTCATAG+TGG - chr4_3:48181173-48181192 Msa0644340:CDS 45.0%
ATTTCGAAGACTCTGTCACG+TGG - chr4_3:48181260-48181279 Msa0644340:CDS 45.0%
! CAGAATCTTCAATGGGCTCA+AGG - chr4_3:48181437-48181456 Msa0644340:intron 45.0%
AGGAGCTTAAAGGGTTGCTT+TGG - chr4_3:48181594-48181613 Msa0644340:intron 45.0%
! GGAGCTTAAAGGGTTGCTTT+GGG - chr4_3:48181595-48181614 Msa0644340:intron 45.0%
!! AAAGGGTTGCTTTGGGATGA+TGG - chr4_3:48181602-48181621 Msa0644340:intron 45.0%
!! AAGGGTTGCTTTGGGATGAT+GGG - chr4_3:48181603-48181622 Msa0644340:intron 45.0%
!! TTGGGATGATGGGGTTTCAT+CGG - chr4_3:48181613-48181632 Msa0644340:intron 45.0%
! AGAAGCACGAGGAGAATCAA+AGG - chr4_3:48181816-48181835 Msa0644340:intron 45.0%
ACGAGGAGAATCAAAGGAGA+TGG - chr4_3:48181822-48181841 Msa0644340:intron 45.0%
! GATGGAAGTGTGAATGGGAT+AGG - chr4_3:48181840-48181859 Msa0644340:intron 45.0%
TTGAAGGAGCAACTAAGTGG+TGG - chr4_3:48181887-48181906 Msa0644340:CDS 45.0%
TGTTGCGGATAAGATGGTGA+TGG - chr4_3:48182000-48182019 Msa0644340:intron 45.0%
! GGGAGATAGTAGAGCTGTTT+TGG - chr4_3:48182102-48182121 Msa0644340:CDS 45.0%
GCCGAACCTGATTATTGGAT+TGG - chr4_3:48182133-48182152 Msa0644340:CDS 45.0%
CGTGGTCAATGATCGTGTTA+AGG - chr4_3:48182428-48182447 Msa0644340:CDS 45.0%
TCGTGTTAAGGGATCTCTCA+AGG - chr4_3:48182440-48182459 Msa0644340:CDS 45.0%
CTCAAGGTTACTCGAGCATT+TGG - chr4_3:48182456-48182475 Msa0644340:CDS 45.0%
AATCCCACACAGTTAACACG+AGG - chr4_3:48182596-48182615 Msa0644340:CDS 45.0%
GCAGCCAATCCAAATCCAAA+AGG - chr4_3:48182649-48182668 Msa0644340:CDS 45.0%
GTTATTGTTTGCAGCCCAAG+TGG - chr4_3:48183027-48183046 Msa0644340:CDS 45.0%
! CAGAAGTGCATTGTTCCACT+TGG + chr4_3:48183045-48183064 None:intergenic 45.0%
CAAGTGGAACAATGCACTTC+TGG - chr4_3:48183043-48183062 Msa0644340:CDS 45.0%
CTTCGACCAAATGTTGAGCA+GGG + chr4_3:48183256-48183275 None:intergenic 45.0%
TCTTCGACCAAATGTTGAGC+AGG + chr4_3:48183257-48183276 None:intergenic 45.0%
! CAATGATGGAAACATCGTCG+TGG + chr4_3:48183481-48183500 None:intergenic 45.0%
AGAAGATAAACGGGTCGGGT+CGG + chr4_3:48180790-48180809 None:intergenic 50.0%
! CGATAACGGCGTTGAAGTGT+TGG + chr4_3:48180874-48180893 None:intergenic 50.0%
CACTTCAACGCCGTTATCGA+CGG - chr4_3:48180875-48180894 Msa0644340:CDS 50.0%
TCCATGAACGCCGTCGATAA+CGG + chr4_3:48180888-48180907 None:intergenic 50.0%
CCGGAAAAAGCCTTCGGAAT+CGG + chr4_3:48180987-48181006 None:intergenic 50.0%
!! CCGATTCCGAAGGCTTTTTC+CGG - chr4_3:48180984-48181003 Msa0644340:intron 50.0%
!! GCTTTTTCCGGTAACTTCGG+TGG - chr4_3:48180996-48181015 Msa0644340:intron 50.0%
ACCAACACCACCGAAGTTAC+CGG + chr4_3:48181006-48181025 None:intergenic 50.0%
GACATGAACCCACGCTCAAT+CGG + chr4_3:48181080-48181099 None:intergenic 50.0%
ATTGAGCGTGGGTTCATGTC+CGG - chr4_3:48181080-48181099 Msa0644340:CDS 50.0%
GATAAAGATAGCTCCGGCAC+CGG - chr4_3:48181137-48181156 Msa0644340:CDS 50.0%
AAGACTCTGTCACGTGGACA+GGG - chr4_3:48181266-48181285 Msa0644340:CDS 50.0%
TCACCAGCAAGAATCCACTC+CGG + chr4_3:48181335-48181354 None:intergenic 50.0%
! AATGGGCTCAAGGGAAAGCA+GGG - chr4_3:48181447-48181466 Msa0644340:intron 50.0%
!! AGGGTTGCTTTGGGATGATG+GGG - chr4_3:48181604-48181623 Msa0644340:intron 50.0%
! TCATCATCGACTGAATCGCG+TGG + chr4_3:48181656-48181675 None:intergenic 50.0%
ATTCTCCTCGTGCTTCTTCG+CGG + chr4_3:48181813-48181832 None:intergenic 50.0%
! GAGCAACTAAGTGGTGGTGA+TGG - chr4_3:48181893-48181912 Msa0644340:CDS 50.0%
!! CAACTAAGTGGTGGTGATGG+TGG - chr4_3:48181896-48181915 Msa0644340:CDS 50.0%
AAGCTCTTTCGCGAGCTTTG+AGG - chr4_3:48181954-48181973 Msa0644340:intron 50.0%
!! GTTACTCGAGCATTTGGTGC+CGG - chr4_3:48182462-48182481 Msa0644340:CDS 50.0%
! TGGTGCCGGATTTCTCAAAC+AGG - chr4_3:48182476-48182495 Msa0644340:CDS 50.0%
CAGCCTCGTGTTAACTGTGT+GGG + chr4_3:48182602-48182621 None:intergenic 50.0%
TCAGCCTCGTGTTAACTGTG+TGG + chr4_3:48182603-48182622 None:intergenic 50.0%
AGGAGACCCTGCTCAACATT+TGG - chr4_3:48183247-48183266 Msa0644340:CDS 50.0%
TTTCGCGCTGCAAAGAAAGC+TGG - chr4_3:48183284-48183303 Msa0644340:CDS 50.0%
GCGAGGGGGTAAGAACAAAA+AGG + chr4_3:48183632-48183651 None:intergenic 50.0%
TCGACACTCGTGAATAGCGA+GGG + chr4_3:48183648-48183667 None:intergenic 50.0%
GAAGATAAACGGGTCGGGTC+GGG + chr4_3:48180789-48180808 None:intergenic 55.0%
!! CGTTGAAGTGTTGGCGCTGA+CGG + chr4_3:48180865-48180884 None:intergenic 55.0%
! TCCGTTGCAACCGATTCCGA+AGG - chr4_3:48180974-48180993 Msa0644340:intron 55.0%
GCCTTCGGAATCGGTTGCAA+CGG + chr4_3:48180978-48180997 None:intergenic 55.0%
TCCGGTAACTTCGGTGGTGT+TGG - chr4_3:48181002-48181021 Msa0644340:intron 55.0%
! GGTAACTTCGGTGGTGTTGG+TGG - chr4_3:48181005-48181024 Msa0644340:intron 55.0%
!! AACTTCGGTGGTGTTGGTGG+TGG - chr4_3:48181008-48181027 Msa0644340:intron 55.0%
! AAACCCACGCTCTAACGGAC+CGG + chr4_3:48181048-48181067 None:intergenic 55.0%
ATGTCCGGTCCGATTGATCG+TGG - chr4_3:48181095-48181114 Msa0644340:CDS 55.0%
AAACTGATCAACGCCGGTGC+CGG + chr4_3:48181153-48181172 None:intergenic 55.0%
! TTTCGGTTCGACCGAGATCG+AGG - chr4_3:48181204-48181223 Msa0644340:CDS 55.0%
GAAGACTCTGTCACGTGGAC+AGG - chr4_3:48181265-48181284 Msa0644340:CDS 55.0%
! TACTCCGATCAAGGGTGTGC+CGG - chr4_3:48181298-48181317 Msa0644340:intron 55.0%
TTCACCGGCACACCCTTGAT+CGG + chr4_3:48181305-48181324 None:intergenic 55.0%
! CAATGGGCTCAAGGGAAAGC+AGG - chr4_3:48181446-48181465 Msa0644340:intron 55.0%
ATGGGCTCAAGGGAAAGCAG+GGG - chr4_3:48181448-48181467 Msa0644340:intron 55.0%
!! GCGAGCTTTGAGGAAAACCG+AGG - chr4_3:48181964-48181983 Msa0644340:intron 55.0%
CCACGGCACAAGGATCATCT+GGG + chr4_3:48182413-48182432 None:intergenic 55.0%
ACCACGGCACAAGGATCATC+TGG + chr4_3:48182414-48182433 None:intergenic 55.0%
CGATCATTGACCACGGCACA+AGG + chr4_3:48182423-48182442 None:intergenic 55.0%
TGTTGAGCAGGGTCTCCTTC+AGG + chr4_3:48183245-48183264 None:intergenic 55.0%
TAACGCCGCCTATCACCTTG+TGG + chr4_3:48183458-48183477 None:intergenic 55.0%
GACACTCGTGAATAGCGAGG+GGG + chr4_3:48183646-48183665 None:intergenic 55.0%
CGACACTCGTGAATAGCGAG+GGG + chr4_3:48183647-48183666 None:intergenic 55.0%
CTCGACACTCGTGAATAGCG+AGG + chr4_3:48183649-48183668 None:intergenic 55.0%
!! TGAAGTGTTGGCGCTGACGG+AGG + chr4_3:48180862-48180881 None:intergenic 60.0%
GCCGTTATCGACGGCGTTCA+TGG - chr4_3:48180884-48180903 Msa0644340:CDS 60.0%
!! TTCGGTGGTGTTGGTGGTGG+TGG - chr4_3:48181011-48181030 Msa0644340:intron 60.0%
!! TCGGTGGTGTTGGTGGTGGT+GGG - chr4_3:48181012-48181031 Msa0644340:intron 60.0%
!! GGTGGTGGGTTTCCGAGTTC+CGG - chr4_3:48181026-48181045 Msa0644340:intron 60.0%
! CGCTCTAACGGACCGGAACT+CGG + chr4_3:48181041-48181060 None:intergenic 60.0%
AGTTCCGGTCCGTTAGAGCG+TGG - chr4_3:48181041-48181060 Msa0644340:intron 60.0%
GTTCCGGTCCGTTAGAGCGT+GGG - chr4_3:48181042-48181061 Msa0644340:intron 60.0%
ATGTCCGGTCCGATTGAGCG+TGG - chr4_3:48181068-48181087 Msa0644340:CDS 60.0%
TGTCCGGTCCGATTGAGCGT+GGG - chr4_3:48181069-48181088 Msa0644340:CDS 60.0%
GAACCCACGCTCAATCGGAC+CGG + chr4_3:48181075-48181094 None:intergenic 60.0%
! GATCAAGGGTGTGCCGGTGA+AGG - chr4_3:48181304-48181323 Msa0644340:intron 60.0%
CCAGCAAGAATCCACTCCGG+TGG + chr4_3:48181332-48181351 None:intergenic 60.0%
! CCACCGGAGTGGATTCTTGC+TGG - chr4_3:48181329-48181348 Msa0644340:intron 60.0%
! AGATGCCGCGAAGAAGCACG+AGG - chr4_3:48181805-48181824 Msa0644340:intron 60.0%
CCCAGATGATCCTTGTGCCG+TGG - chr4_3:48182410-48182429 Msa0644340:CDS 60.0%
!! CGGAGGCGCCGGAGATTGTT+CGG + chr4_3:48180845-48180864 None:intergenic 65.0%
! TGTGCCGGTGAAGGAGCCAC+CGG - chr4_3:48181313-48181332 Msa0644340:intron 65.0%
CACTCCGGTGGCTCCTTCAC+CGG + chr4_3:48181320-48181339 None:intergenic 65.0%
CGGTGAAGGAGCCACCGGAG+TGG - chr4_3:48181318-48181337 Msa0644340:intron 70.0%
!! GTTGGCGCTGACGGAGGCGC+CGG + chr4_3:48180856-48180875 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 48180443 48183906 48180443 ID=Msa0644340;Name=Msa0644340
chr4_3 mRNA 48180443 48183906 48180443 ID=Msa0644340-mRNA-1;Parent=Msa0644340;Name=Msa0644340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=211|1|1|1|1|1|4|365|712
chr4_3 exon 48180443 48180923 48180443 ID=Msa0644340-mRNA-1:exon:11405;Parent=Msa0644340-mRNA-1
chr4_3 exon 48181044 48181308 48181044 ID=Msa0644340-mRNA-1:exon:11404;Parent=Msa0644340-mRNA-1
chr4_3 exon 48181852 48181965 48181852 ID=Msa0644340-mRNA-1:exon:11403;Parent=Msa0644340-mRNA-1
chr4_3 exon 48182052 48183906 48182052 ID=Msa0644340-mRNA-1:exon:11402;Parent=Msa0644340-mRNA-1
chr4_3 five_prime_UTR 48183696 48183906 48183696 ID=Msa0644340-mRNA-1:five_prime_utr;Parent=Msa0644340-mRNA-1
chr4_3 CDS 48182052 48183695 48182052 ID=Msa0644340-mRNA-1:cds;Parent=Msa0644340-mRNA-1
chr4_3 CDS 48181852 48181965 48181852 ID=Msa0644340-mRNA-1:cds;Parent=Msa0644340-mRNA-1
chr4_3 CDS 48181044 48181308 48181044 ID=Msa0644340-mRNA-1:cds;Parent=Msa0644340-mRNA-1
chr4_3 CDS 48180808 48180923 48180808 ID=Msa0644340-mRNA-1:cds;Parent=Msa0644340-mRNA-1
chr4_3 three_prime_UTR 48180443 48180807 48180443 ID=Msa0644340-mRNA-1:three_prime_utr;Parent=Msa0644340-mRNA-1
Gene Sequence

>Msa0644340

ATGGGAAACAAAATAGGAAAATTAACGGTATGTTTCACCGGAAACAACAACAGTAACCGTAAACACGACATATCCATATTAATAACGGATCCTTTAGATGAAGGACTTGGTCACTCTTTCTGTTACGTTAGACCCGACCCGACCCGTTTATCTTCTTCCAAAGTTCACTCTGAAGAAACCACAACTTTCCGAACAATCTCCGGCGCCTCCGTCAGCGCCAACACTTCAACGCCGTTATCGACGGCGTTCATGGATTTATACTCCTACGGTTGTTTTGATAGAGCTGCAGCTTTTGAAAGTTCAACTTCTTTTGCTTCTCTTCCGTTGCAACCGATTCCGAAGGCTTTTTCCGGTAACTTCGGTGGTGTTGGTGGTGGTGGGTTTCCGAGTTCCGGTCCGTTAGAGCGTGGGTTTATGTCCGGTCCGATTGAGCGTGGGTTCATGTCCGGTCCGATTGATCGTGGTTTATTTTCCGGTCCGATGGATAAAGATAGCTCCGGCACCGGCGTTGATCAGTTTCAGAGAAGCTTCTCTCATAGTGGATTAGGGTTTTCGGTTCGACCGAGATCGAGGAAAGAAAAATGGATTCGTGTTCTTCAACGTGCGATTTCGAAGACTCTGTCACGTGGACAGGGTTCTATTGTTACTCCGATCAAGGGTGTGCCGGTGAAGGAGCCACCGGAGTGGATTCTTGCTGGTGAGAAGCACAATGAGAATTTGACTGTGAGTAGTTTGAATTTGAGTAGTGAAGGTAGTTTGGAAGATGATGATTCGTTGGGAAGTCAGAATCTTCAATGGGCTCAAGGGAAAGCAGGGGAAGATCGTGTTCATGTTGTTGTTTCTGAGGAACATGGTTGGGTTTTTGTTGGGATTTATGATGGTTTTAATGGTCCTGATGCACCTGATTATTTGTTGTCAAATCTTTATAGTTTTGTTCATAAGGAGCTTAAAGGGTTGCTTTGGGATGATGGGGTTTCATCGGATAATTCGAATTTGAAACCACGCGATTCAGTCGATGATGATGTTGTTGATGGTGTTGTTGTGAAAGAGGATATGTTGTTTGATGATTGTTCTCAATGTGTTGATCAAGAGAAAGGTGATTCGATTTCAAAGAAAAAAAAGAGAGGTAAGAATTCGAAGAATAAGTACAAAGATGCCGCGAAGAAGCACGAGGAGAATCAAAGGAGATGGAAGTGTGAATGGGATAGGGAGAGATTAGAGCTTGATAGAAGATTGAAGGAGCAACTAAGTGGTGGTGATGGTGGCGATGATAATTCTGTTAATCATTCAGATGTTTTGGAAGCTCTTTCGCGAGCTTTGAGGAAAACCGAGGAGTCTTATCTTGATGTTGCGGATAAGATGGTGATGGAGAATCCGGAACTTGCTTTAATGGGTTCATGTGTTCTTGTAATGTTGATGAAAGGGGAAGATGTTTATGTGATGAATGTGGGAGATAGTAGAGCTGTTTTGGCACAGAAAGCCGAACCTGATTATTGGATTGGGAAAATTAGACAGGATTTGGAGAGGATTAATGAGGAGACAATGCATGATCTTGAATCATGGGATGATGCTGATAAATCAAATGTTGTTCCTAGTTTAAGTGCTTTTCAGCTTACCAAGGATCATAGTACCAATGTTGAAGAGGAAGTAATCAGAATAATAAAAGAGCACCCAGATGATCCTTGTGCCGTGGTCAATGATCGTGTTAAGGGATCTCTCAAGGTTACTCGAGCATTTGGTGCCGGATTTCTCAAACAGCCCAAGTGGAACAATGCACTTCTGGAGATGTTTAGAATAGACTACGTAGGAAATTCTCCATACATTACTTGCCAACCATATCTTAAACACCACAGATTAGGCCAAAAAGACAAATTTTTGATTCTATGCTCCGACGGACTCTATCAATACTTGTCAAACGAAGAAGCAGTAGCTGAAGTTGAGCTTTTCATCACATTACAACCTGAAGGAGACCCTGCTCAACATTTGGTCGAAGAAGTCTTGTTTCGCGCTGCAAAGAAAGCTGGTTTGGACTTTCATGAATTGCTCGAAATTCCACAAGGTGATAGGCGGCGTTACCACGACGATGTTTCCATCATTGTTATTTCTTTGGAAGGAAGGATATGGAGATCATGTGTATAA
Protein sequence

>Msa0644340

MGNKIGKLTVCFTGNNNSNRKHDISILITDPLDEGLGHSFCYVRPDPTRLSSSKVHSEETTTFRTISGASVSANTSTPLSTAFMDLYSYGCFDRAAAFESSTSFASLPLQPIPKAFSGNFGGVGGGGFPSSGPLERGFMSGPIERGFMSGPIDRGLFSGPMDKDSSGTGVDQFQRSFSHSGLGFSVRPRSRKEKWIRVLQRAISKTLSRGQGSIVTPIKGVPVKEPPEWILAGEKHNENLTVSSLNLSSEGSLEDDDSLGSQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLSNLYSFVHKELKGLLWDDGVSSDNSNLKPRDSVDDDVVDGVVVKEDMLFDDCSQCVDQEKGDSISKKKKRGKNSKNKYKDAAKKHEENQRRWKCEWDRERLELDRRLKEQLSGGDGGDDNSVNHSDVLEALSRALRKTEESYLDVADKMVMENPELALMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKAEPDYWIGKIRQDLERINEETMHDLESWDDADKSNVVPSLSAFQLTKDHSTNVEEEVIRIIKEHPDDPCAVVNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGNSPYITCQPYLKHHRLGQKDKFLILCSDGLYQYLSNEEAVAEVELFITLQPEGDPAQHLVEEVLFRAAKKAGLDFHELLEIPQGDRRRYHDDVSIIVISLEGRIWRSCV*