Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950680 | sp|Q2R2D5|XA21_ORYSJ | 33.043 | 1032 | 530 | 22 | 30 | 946 | 35 | 1020 | 1.85e-143 | 456 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950680 | A0A072V4N4 | 58.456 | 1218 | 236 | 18 | 1 | 951 | 1 | 1215 | 0.0 | 1239 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0311330 | Msa0950680 | 0.813864 | 2.062749e-51 | -8.615850e-47 |
| Msa0452010 | Msa0950680 | 0.832260 | 1.046027e-55 | -8.615850e-47 |
| Msa0599250 | Msa0950680 | 0.820502 | 6.632333e-53 | -8.615850e-47 |
| Msa0607440 | Msa0950680 | 0.807719 | 4.406483e-50 | -8.615850e-47 |
| Msa0630000 | Msa0950680 | 0.814735 | 1.324090e-51 | -8.615850e-47 |
| Msa0718920 | Msa0950680 | 0.818766 | 1.651801e-52 | -8.615850e-47 |
| Msa0752580 | Msa0950680 | 0.806006 | 1.014132e-49 | -8.615850e-47 |
| Msa0754500 | Msa0950680 | 0.808223 | 3.442596e-50 | -8.615850e-47 |
| Msa0813030 | Msa0950680 | 0.812484 | 4.142810e-51 | -8.615850e-47 |
| Msa0814780 | Msa0950680 | 0.807216 | 5.632947e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950680 | MtrunA17_Chr2g0284121 | 58.456 | 1218 | 236 | 18 | 1 | 951 | 1 | 1215 | 0.0 | 1239 |
| Msa0950680 | MtrunA17_Chr3g0095201 | 61.881 | 989 | 218 | 12 | 121 | 950 | 1 | 989 | 0.0 | 1105 |
| Msa0950680 | MtrunA17_Chr3g0095201 | 29.423 | 520 | 277 | 12 | 72 | 515 | 99 | 604 | 1.01e-37 | 153 |
| Msa0950680 | MtrunA17_Chr0c09g0493531 | 67.221 | 842 | 135 | 8 | 250 | 950 | 5 | 846 | 0.0 | 1035 |
| Msa0950680 | MtrunA17_Chr0c09g0493531 | 27.922 | 308 | 177 | 5 | 67 | 374 | 210 | 472 | 9.84e-22 | 101 |
| Msa0950680 | MtrunA17_Chr2g0284191 | 65.238 | 840 | 128 | 8 | 251 | 951 | 24 | 838 | 0.0 | 994 |
| Msa0950680 | MtrunA17_Chr2g0284191 | 26.409 | 337 | 201 | 6 | 67 | 403 | 203 | 492 | 2.09e-22 | 103 |
| Msa0950680 | MtrunA17_Chr8g0363691 | 54.341 | 1025 | 303 | 19 | 83 | 947 | 227 | 1246 | 0.0 | 952 |
| Msa0950680 | MtrunA17_Chr8g0363691 | 30.657 | 685 | 335 | 21 | 23 | 597 | 18 | 672 | 3.36e-55 | 208 |
| Msa0950680 | MtrunA17_Chr8g0363691 | 32.361 | 377 | 203 | 13 | 274 | 617 | 66 | 423 | 2.39e-26 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950680 | AT5G20480.1 | 32.039 | 1030 | 548 | 30 | 26 | 950 | 19 | 1001 | 2.29e-137 | 437 |
| Msa0950680 | AT5G20480.2 | 32.039 | 1030 | 548 | 30 | 26 | 950 | 19 | 1001 | 2.29e-137 | 437 |
| Msa0950680 | AT3G47570.1 | 33.919 | 911 | 418 | 23 | 220 | 951 | 98 | 1003 | 6.85e-136 | 433 |
| Msa0950680 | AT3G47090.1 | 30.694 | 1023 | 557 | 23 | 37 | 951 | 24 | 1002 | 8.87e-135 | 430 |
| Msa0950680 | AT5G46330.2 | 33.163 | 977 | 505 | 27 | 78 | 946 | 221 | 1157 | 1.10e-134 | 434 |
| Msa0950680 | AT5G46330.2 | 31.402 | 656 | 321 | 18 | 17 | 597 | 12 | 613 | 4.34e-67 | 245 |
| Msa0950680 | AT5G46330.2 | 32.051 | 312 | 177 | 6 | 72 | 350 | 454 | 763 | 1.31e-30 | 130 |
| Msa0950680 | AT5G46330.1 | 33.163 | 977 | 505 | 27 | 78 | 946 | 221 | 1157 | 1.10e-134 | 434 |
| Msa0950680 | AT5G46330.1 | 31.402 | 656 | 321 | 18 | 17 | 597 | 12 | 613 | 4.34e-67 | 245 |
| Msa0950680 | AT5G46330.1 | 32.051 | 312 | 177 | 6 | 72 | 350 | 454 | 763 | 1.31e-30 | 130 |
Find 177 sgRNAs with CRISPR-Local
Find 797 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCCAATAATGTGTTAC+TGG | 0.335258 | 6_3:+39481177 | Msa0950680:CDS |
| AAAGGAGATGTGTACAGCTA+TGG | 0.460020 | 6_3:+39481536 | Msa0950680:CDS |
| AAATATCATTTGGAATAAAG+GGG | 0.575471 | 6_3:-39481905 | None:intergenic |
| AACCTATGTTGAGAAATTCG+AGG | 0.550915 | 6_3:-39479641 | None:intergenic |
| AACTTGGATTAGTGAATCAA+TGG | 0.486628 | 6_3:+39481631 | Msa0950680:CDS |
| AAGAAGTGCAAATTCATCGG+TGG | 0.700699 | 6_3:-39471087 | None:intergenic |
| AAGAATATGAGAAGGGTCTA+AGG | 0.381561 | 6_3:-39471129 | None:intergenic |
| AAGGAGATGTGTACAGCTAT+GGG | 0.538595 | 6_3:+39481537 | Msa0950680:CDS |
| AAGGGAGTCATTTCAGTTAA+AGG | 0.264595 | 6_3:+39481518 | Msa0950680:CDS |
| AATGAAGATAGAGTGATCTA+AGG | 0.354629 | 6_3:-39472979 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGAAGTAATAATAGAGTG+AGG | - | chr6_3:39471030-39471049 | None:intergenic | 30.0% | |
| TTCTTTCTCTTCATGCAATT+GGG | + | chr6_3:39471181-39471200 | Msa0950680:CDS | 30.0% | |
| AAAGAAAGATTTCCCAATTG+AGG | - | chr6_3:39471287-39471306 | None:intergenic | 30.0% | |
| GTTATCAAAACTTCAGCAAT+TGG | + | chr6_3:39471442-39471461 | Msa0950680:intron | 30.0% | |
| AAACTTCAGCAATTGGATAT+TGG | + | chr6_3:39471449-39471468 | Msa0950680:intron | 30.0% | |
| ATACTCTAACATTGACAAGT+TGG | - | chr6_3:39471513-39471532 | None:intergenic | 30.0% | |
| TTAGCAAATAACTCCTTATC+GGG | + | chr6_3:39471686-39471705 | Msa0950680:CDS | 30.0% | |
| TGAATATATCATACCCGATA+AGG | - | chr6_3:39471702-39471721 | None:intergenic | 30.0% | |
| TCATGCATGTTATTATGGAA+TGG | - | chr6_3:39471727-39471746 | None:intergenic | 30.0% | |
| CTCAATCATGCATGTTATTA+TGG | - | chr6_3:39471732-39471751 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 39470981 | 39482036 | 39470981 | ID=Msa0950680;Name=Msa0950680 |
| chr6_3 | mRNA | 39470981 | 39482036 | 39470981 | ID=Msa0950680-mRNA-1;Parent=Msa0950680;Name=Msa0950680-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.30|0.14|1|0.23|0.14|14|210|951 |
| chr6_3 | exon | 39470981 | 39471206 | 39470981 | ID=Msa0950680-mRNA-1:exon:9537;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39471528 | 39471707 | 39471528 | ID=Msa0950680-mRNA-1:exon:9538;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39472804 | 39472923 | 39472804 | ID=Msa0950680-mRNA-1:exon:9539;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39472942 | 39473163 | 39472942 | ID=Msa0950680-mRNA-1:exon:9540;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39478991 | 39479209 | 39478991 | ID=Msa0950680-mRNA-1:exon:9541;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39479316 | 39479549 | 39479316 | ID=Msa0950680-mRNA-1:exon:9542;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39479580 | 39479762 | 39479580 | ID=Msa0950680-mRNA-1:exon:9543;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39479898 | 39480058 | 39479898 | ID=Msa0950680-mRNA-1:exon:9544;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39480173 | 39480418 | 39480173 | ID=Msa0950680-mRNA-1:exon:9545;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39480488 | 39480728 | 39480488 | ID=Msa0950680-mRNA-1:exon:9546;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39480753 | 39480986 | 39480753 | ID=Msa0950680-mRNA-1:exon:9547;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39481005 | 39481059 | 39481005 | ID=Msa0950680-mRNA-1:exon:9548;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39481096 | 39481310 | 39481096 | ID=Msa0950680-mRNA-1:exon:9549;Parent=Msa0950680-mRNA-1 |
| chr6_3 | exon | 39481507 | 39482036 | 39481507 | ID=Msa0950680-mRNA-1:exon:9550;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39470981 | 39471206 | 39470981 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39471528 | 39471707 | 39471528 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39472804 | 39472923 | 39472804 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39472942 | 39473163 | 39472942 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39478991 | 39479209 | 39478991 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39479316 | 39479549 | 39479316 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39479580 | 39479762 | 39479580 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39479898 | 39480058 | 39479898 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39480173 | 39480418 | 39480173 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39480488 | 39480728 | 39480488 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39480753 | 39480986 | 39480753 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39481005 | 39481059 | 39481005 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39481096 | 39481310 | 39481096 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | CDS | 39481507 | 39481826 | 39481507 | ID=Msa0950680-mRNA-1:cds;Parent=Msa0950680-mRNA-1 |
| chr6_3 | three_prime_UTR | 39481827 | 39482036 | 39481827 | ID=Msa0950680-mRNA-1:three_prime_utr;Parent=Msa0950680-mRNA-1 |
| Gene Sequence |
>Msa0950680 ATGGCCAAATTATACTCTTGTCACTCATGTCTCTGTATTCTCTCTGCCTCACTCTATTATTACTTCTTCTTCACTTGCTTAGTTGTGAGCAGTGAAAAAAACATAACCACCGATGAATTTGCACTTCTTGCATTCAAATCCTCAATCACCTTAGACCCTTCTCATATTCTTAGTAATTGGTCAATTTACTCTTCTGCTTCTTCTTTCTCTTCATGCAATTGGGTTGAGTATCTAAGTTTGAAATCAAATCACATCAATGGAACCATTCCATATGCTATTGGTCAACTTCGTCAGTTGAGGTTTTTGAGTCTTAGTAATAACACGCTGTTGTCTGGAGTCATACCAACAACAATTTGCAACATGTCTTCTCTTGAAGAAATTCATTTAGCAAATAACTCCTTATCGGGAGAAATTCCGAAAGAAATTGGTGATGTTACACAGCTGAAAATGGTCATACTTCAACATAATTTCCTCAGAGGCAACATATTAATGTTCAACAACTCATCGTTGCAAATTTTGGCACTTGGTATCCTTCCATCAAATGTTTGTCAAGGGCTTCCAAACCTTAGATCACTCTATCTTCATTTTAATGATTTTTCTGGTGAGATGCCAAATGTTTGGCGTTATTGCAAAGAGTTAGAATATTTGGTACTATCCTTCAATAATTTTGACAAAGGACGTATGCCCGCTGACATTGGAAAATTGACCAATCTTCAAATTTTGTATCTCGCAAACAACAACTTGGAAGGTGAAATTCCATTCTCCTTCTTCAACATCTCTTCTTTGCGAGTGATCAACCTTGATGGTAACAATATAAATGGAACTCTTCCAGATGAGATGTGTAATCAACTTCCTCAACTTGAATGGTTTAATCTAAACAATAATTATCTTGAAGGAACCATTCCACGATCGATTGGCAACTGCACATCGTTACAAAATTTATATTTAGATAATAACTTGTTTTCAGGTTCGATACCATTGGAGATTAGTCATCTCAGTCAACTTCAGATTCTACAAATGGGACATAATAACTTGAGTGGACCTATTCCTTCGAAACTCTTCAACATTTCAACATTGGAAATTTTGTATCTTGCACAAAATTCTTTCACAGGCATGCTTCCATCAAATTTGGGATTCGGACTTCCTAACCTATACTTCCTGAGTATGCGTGGAAACAAGTTTGTTGGAAAAATTCCAAATATTGACATTAATGATAATGAATTTAGTGGAATTATATCTAATTCTTTTGGAGATTTAAGACTCCTCGAATTTCTCAACATAGGTTTCAATAACTTGGCATTAATTGATGATTCTCTTGAAGTAAACTTCCTTACTTCGCTTACAAGTTGCAGATATCTGAAATATCTTGGAGTAGCAGTGAATAGTAATGATTTACATGGATCAATCCCTAGTGCAATTAAAGGGTTACATAAACTTCAATTTTTAGATCTTGGTTACAATGGACTGCAAGGATCCATGATTGATGAGCTTTGTGAAATTAAGAGTTTGAGTGAATTGTATTTAGCAAGCAATAAGCTTTTTGGAGTAAACTTGTCCTCTAATGCTTTAACTGGAAATCTTCCAATGGAGATTAGTAATTTAAGAGCAATTTTGCTATTAGATTTTTCAAGAAATCAAATTTCAAGCAACATTCCTGAAACCATAGGTTCGTTAACAACTCTGCAGAATCTCTCCTTAGCAAAAAACAAATTGTATGGGAAAATTCCCACAACACTTGGTGAATTGGTAAGCTTGAGCAGCTTGGACTTGTCCGATAATCTTCTTACCGGTGGAGAGATTCCGGATGGTGGCCAATTCAAAGATTTCACTGCTCAATCATTCATGCATAATGAAGCACTTTGTGGCAATCCTCACTTGCAAGTACCTCCATGTGATCAACATAGGAAAAAGTCAAAGATGCAATCAATCATATGCATATCGTGCATAATTGTTGTGTTAGGCATTTTGGTTGTTGCATGCATAATACTTCGAATTCATAAAAGGAGAAATGTTGAAAATCCACTTGAAAGGGGAGTTCCAGAAAGGATTTCCTATTATGAACTCGTCCAAGCAACTAATGGATTCAGTGAGAGTAATTTACTTGGAAGGGGTGGATTTGGATCTGTGTATCAAGGAATGCTTTCTAGTGGGAAGATGATAGCAGTTAAAGTACTTGACTTGACAATGGAAGCAGCATCAAGAAGCTTTGATGCAGAATGCAATGCAATGTGCAACCTTCGGCATCGAAATCTCGTTCAGATTATCAATTTCAAATATCTAGTGATGGAGTTTATGTCAAATGGAAGTGTGGAAAAATGGTTGTTGAACATAATGATTGATGTGGCATCTGCATTGGAGTATCTCCATCATGGTTCTTCAATACCAGTGGTTCATTGTGATTTAAAGCCCAATAATGTGTTACTGGATGAAGATATGGTTGCACATGTAAGTGATTTTGGGATTTCCAAGCTCTTGGATGAAGGACAATCAAAAACTCATACAAGGACTTTAGCTACTCTTGGCTATGTTGCACCAGAGTATGGATCTAAGGGAGTCATTTCAGTTAAAGGAGATGTGTACAGCTATGGGATAATGCTGATGGAAATGTTCACCGGAAAGAAGCCGACAAATGAAATGTTTTCAGCAGAATTAACCTTGAAAACTTGGATTAGTGAATCAATGGTCAATCCCAGTGTGGAAGTTGTGGATTCCAATTTAATCTCACAACATGGGAAAGAAATTCATGATATCTTAACTTTAGCATTGAGATGTTGTGAAGATTCACCAGAAGCACGAAATAGTATGACAGATGTAATTGCGTCATTAATCAAGATCAAGACCTCATTCATCCGATGA |
| Protein sequence |
>Msa0950680 MAKLYSCHSCLCILSASLYYYFFFTCLVVSSEKNITTDEFALLAFKSSITLDPSHILSNWSIYSSASSFSSCNWVEYLSLKSNHINGTIPYAIGQLRQLRFLSLSNNTLLSGVIPTTICNMSSLEEIHLANNSLSGEIPKEIGDVTQLKMVILQHNFLRGNILMFNNSSLQILALGILPSNVCQGLPNLRSLYLHFNDFSGEMPNVWRYCKELEYLVLSFNNFDKGRMPADIGKLTNLQILYLANNNLEGEIPFSFFNISSLRVINLDGNNINGTLPDEMCNQLPQLEWFNLNNNYLEGTIPRSIGNCTSLQNLYLDNNLFSGSIPLEISHLSQLQILQMGHNNLSGPIPSKLFNISTLEILYLAQNSFTGMLPSNLGFGLPNLYFLSMRGNKFVGKIPNIDINDNEFSGIISNSFGDLRLLEFLNIGFNNLALIDDSLEVNFLTSLTSCRYLKYLGVAVNSNDLHGSIPSAIKGLHKLQFLDLGYNGLQGSMIDELCEIKSLSELYLASNKLFGVNLSSNALTGNLPMEISNLRAILLLDFSRNQISSNIPETIGSLTTLQNLSLAKNKLYGKIPTTLGELVSLSSLDLSDNLLTGGEIPDGGQFKDFTAQSFMHNEALCGNPHLQVPPCDQHRKKSKMQSIICISCIIVVLGILVVACIILRIHKRRNVENPLERGVPERISYYELVQATNGFSESNLLGRGGFGSVYQGMLSSGKMIAVKVLDLTMEAASRSFDAECNAMCNLRHRNLVQIINFKYLVMEFMSNGSVEKWLLNIMIDVASALEYLHHGSSIPVVHCDLKPNNVLLDEDMVAHVSDFGISKLLDEGQSKTHTRTLATLGYVAPEYGSKGVISVKGDVYSYGIMLMEMFTGKKPTNEMFSAELTLKTWISESMVNPSVEVVDSNLISQHGKEIHDILTLALRCCEDSPEARNSMTDVIASLIKIKTSFIR* |
