AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0965070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0965070 MtrunA17_Chr6g0485851 97.196 107 3 0 1 107 1 107 6.74e-74 220
Msa0965070 MtrunA17_Chr8g0348041 38.393 112 64 2 1 107 1 112 3.58e-18 78.2
Msa0965070 MtrunA17_Chr7g0275191 42.708 96 53 2 19 113 5 99 8.41e-18 77.0
Msa0965070 MtrunA17_Chr3g0127011 35.556 90 57 1 19 107 24 113 1.74e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0965070 AT2G46225.3 47.917 96 48 2 19 113 20 114 4.90e-24 94.0
Msa0965070 AT2G46225.1 47.917 96 48 2 19 113 20 114 5.72e-24 94.0
Msa0965070 AT2G46225.2 47.475 99 50 2 16 113 17 114 6.39e-24 94.4
Msa0965070 AT5G42030.3 37.778 90 55 1 19 107 22 111 4.13e-15 68.9
Msa0965070 AT5G42030.1 37.778 90 55 1 19 107 22 111 4.59e-15 69.7
Msa0965070 AT5G42030.2 37.778 90 55 1 19 107 22 111 9.57e-15 69.3
Msa0965070 AT3G49290.1 36.667 90 56 1 19 107 15 104 8.94e-14 66.6
Msa0965070 AT3G49290.2 36.667 90 56 1 19 107 15 104 8.94e-14 66.6
Msa0965070 AT5G24310.2 34.444 90 58 1 19 107 15 104 3.51e-13 64.3
Msa0965070 AT5G24310.3 34.444 90 58 1 19 107 21 110 9.64e-13 63.5
Msa0965070 AT5G24310.4 34.444 90 58 1 19 107 15 104 1.09e-12 63.5
Msa0965070 AT5G24310.1 34.444 90 58 1 19 107 21 110 1.13e-12 63.5

Find 32 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCAAATTCAATAATT+TGG 0.131293 6_3:+92293458 None:intergenic
GCACTTTCTTAAGTCTCTTC+AGG 0.315239 6_3:-92295381 Msa0965070:intron
ATGCATTTGTGTGAGAAATA+AGG 0.339283 6_3:+92293694 None:intergenic
TTTAAAAGCAACAAAGATAA+GGG 0.362021 6_3:+92295537 None:intergenic
CATACTTTGAATCTGAAATA+TGG 0.364220 6_3:+92293559 None:intergenic
CACTAACTTGTGTGAGGCAT+TGG 0.368809 6_3:+92293649 None:intergenic
TCACATCCATGTTACTGCTT+TGG 0.380781 6_3:+92295462 None:intergenic
AATATTTGAAAGTAACCATA+TGG 0.391529 6_3:+92293489 None:intergenic
TTACTGTGAAACAACATTCA+TGG 0.406311 6_3:-92295207 Msa0965070:CDS
AATAAGGCCTTCAAGATTAG+AGG 0.412741 6_3:+92293710 None:intergenic
AGACACTGACTGATGCATAT+TGG 0.428140 6_3:+92293535 None:intergenic
AATGTTTCCTCTAATCTTGA+AGG 0.436804 6_3:-92293717 Msa0965070:CDS
TTTAGGAGTTAAAAGAATTG+AGG 0.456988 6_3:-92295253 Msa0965070:intron
GTCACTGTAGTTGATCATCT+TGG 0.468892 6_3:-92293741 Msa0965070:CDS
TTGAAAGTAACCATATGGAT+TGG 0.478476 6_3:+92293494 None:intergenic
ATCAGAAGCTGAACCTGAAG+TGG 0.499468 6_3:-92295414 Msa0965070:CDS
CTTCTTCCAAAGCAGTAACA+TGG 0.526347 6_3:-92295468 Msa0965070:exon
CTAGGTGAAACCAATCCATA+TGG 0.535048 6_3:-92293504 Msa0965070:three_prime_UTR
AGAATATATATGCAGAGCTA+TGG 0.539583 6_3:-92293763 Msa0965070:CDS
CTGAAATATGGTCCTACGTG+TGG 0.567048 6_3:+92293571 None:intergenic
AAAAGAAGAACAACATGTCA+CGG 0.579556 6_3:-92295678 None:intergenic
CATTCACATAGTCGTGGCAT+CGG 0.592647 6_3:-92293420 Msa0965070:three_prime_UTR
AGACAATTCAAAACATGGTG+TGG 0.595846 6_3:+92295622 None:intergenic
AAAGAAGAACAACATGTCAC+GGG 0.609171 6_3:-92295677 None:intergenic
GTCAGTGTCTGTCTGACACT+AGG 0.610445 6_3:-92293522 Msa0965070:three_prime_UTR
TTGGAAGAAGAAATCAACAA+AGG 0.614846 6_3:+92295481 None:intergenic
TTAAGAGACAATTCAAAACA+TGG 0.657374 6_3:+92295617 None:intergenic
ATGCCACGACTATGTGAATG+TGG 0.657538 6_3:+92293423 None:intergenic
ATAAGGGATTATGTCACATG+TGG 0.672740 6_3:+92295553 None:intergenic
TAATAGCACTAACTTGTGTG+AGG 0.677548 6_3:+92293643 None:intergenic
AGTCCACATTCACATAGTCG+TGG 0.683060 6_3:-92293426 Msa0965070:three_prime_UTR
TCTCATTATAATCCACACGT+AGG 0.730493 6_3:-92293583 Msa0965070:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTGAAATTGAATATTT+TGG - chr6_3:92293788-92293807 Msa0965070:intron 10.0%
!!! TTTATTGAAATTGAATATTT+TGG - chr6_3:92293788-92293807 Msa0965070:intron 10.0%
!!! TTTTAAAACATTGTTTTACT+TGG - chr6_3:92294567-92294586 Msa0965070:intron 15.0%
!! ATTAATTCAACAATTAAGAT+AGG - chr6_3:92294864-92294883 Msa0965070:intron 15.0%
!! ATAGGTGTTTAATATTATTA+AGG - chr6_3:92294928-92294947 Msa0965070:intron 15.0%
!!! TTTTTCCAAATTATTGAATT+TGG - chr6_3:92295611-92295630 Msa0965070:five_prime_UTR 15.0%
!! AAAATCCAAATTCAATAATT+TGG + chr6_3:92295619-92295638 None:intergenic 15.0%
!!! TTTTAAAACATTGTTTTACT+TGG - chr6_3:92294567-92294586 Msa0965070:intron 15.0%
!! ATTAATTCAACAATTAAGAT+AGG - chr6_3:92294864-92294883 Msa0965070:intron 15.0%
!! ATAGGTGTTTAATATTATTA+AGG - chr6_3:92294928-92294947 Msa0965070:intron 15.0%
!!! TTTTTCCAAATTATTGAATT+TGG - chr6_3:92295611-92295630 Msa0965070:five_prime_UTR 15.0%
!! AAAATCCAAATTCAATAATT+TGG + chr6_3:92295619-92295638 None:intergenic 15.0%
!! TTTAAAAGCAACAAAGATAA+GGG + chr6_3:92293540-92293559 None:intergenic 20.0%
!!! TTTTAAAAGCAACAAAGATA+AGG + chr6_3:92293541-92293560 None:intergenic 20.0%
!!! GAAATTGAATATTTTGGTTA+TGG - chr6_3:92293794-92293813 Msa0965070:intron 20.0%
!!! ATTTTGGTTATGGTTTTTTT+AGG - chr6_3:92293804-92293823 Msa0965070:intron 20.0%
!!! TTCATTTTGTTTATGTATCT+TGG - chr6_3:92293983-92294002 Msa0965070:intron 20.0%
!!! TCAATTCTCAATTTTTGTTT+TGG - chr6_3:92294641-92294660 Msa0965070:intron 20.0%
!!! CAATTCTCAATTTTTGTTTT+GGG - chr6_3:92294642-92294661 Msa0965070:intron 20.0%
!!! TCAAGTTGATTTAGATTAAT+TGG + chr6_3:92294746-92294765 None:intergenic 20.0%
!! ATTTGATTGAGTCTAAAAAA+TGG + chr6_3:92294790-92294809 None:intergenic 20.0%
!! AGTCATTTGGATTAATTAAA+TGG - chr6_3:92294813-92294832 Msa0965070:intron 20.0%
!! TATCTATGGACAAATAATAT+AGG - chr6_3:92294910-92294929 Msa0965070:intron 20.0%
!! AATATTTGAAAGTAACCATA+TGG + chr6_3:92295588-92295607 None:intergenic 20.0%
!! TTTAAAAGCAACAAAGATAA+GGG + chr6_3:92293540-92293559 None:intergenic 20.0%
!!! TTTTAAAAGCAACAAAGATA+AGG + chr6_3:92293541-92293560 None:intergenic 20.0%
!!! GAAATTGAATATTTTGGTTA+TGG - chr6_3:92293794-92293813 Msa0965070:intron 20.0%
!!! ATTTTGGTTATGGTTTTTTT+AGG - chr6_3:92293804-92293823 Msa0965070:intron 20.0%
!!! TTCATTTTGTTTATGTATCT+TGG - chr6_3:92293983-92294002 Msa0965070:intron 20.0%
!!! TCAATTCTCAATTTTTGTTT+TGG - chr6_3:92294641-92294660 Msa0965070:intron 20.0%
!!! CAATTCTCAATTTTTGTTTT+GGG - chr6_3:92294642-92294661 Msa0965070:intron 20.0%
!!! TCAAGTTGATTTAGATTAAT+TGG + chr6_3:92294746-92294765 None:intergenic 20.0%
!! ATTTGATTGAGTCTAAAAAA+TGG + chr6_3:92294790-92294809 None:intergenic 20.0%
!! AGTCATTTGGATTAATTAAA+TGG - chr6_3:92294813-92294832 Msa0965070:intron 20.0%
!! TATCTATGGACAAATAATAT+AGG - chr6_3:92294910-92294929 Msa0965070:intron 20.0%
!! AATATTTGAAAGTAACCATA+TGG + chr6_3:92295588-92295607 None:intergenic 20.0%
! TTAAGAGACAATTCAAAACA+TGG + chr6_3:92293460-92293479 None:intergenic 25.0%
! TGTGTTATGTTCTGTTATTA+TGG - chr6_3:92293735-92293754 Msa0965070:CDS 25.0%
! TTTAGGAGTTAAAAGAATTG+AGG - chr6_3:92293821-92293840 Msa0965070:intron 25.0%
! TTTAAAAAACTGAAACGAAC+CGG - chr6_3:92294105-92294124 Msa0965070:intron 25.0%
!!! TTTTTGAGTTATGGTTCAAT+TGG + chr6_3:92294527-92294546 None:intergenic 25.0%
!!! TCGATTAGATTTTTGAGTTA+TGG + chr6_3:92294536-92294555 None:intergenic 25.0%
! AAAATTGTACATTTGTGTAG+TGG - chr6_3:92294668-92294687 Msa0965070:intron 25.0%
! AAATTGTACATTTGTGTAGT+GGG - chr6_3:92294669-92294688 Msa0965070:intron 25.0%
! TAATCTAAATCAACTTGAGT+TGG - chr6_3:92294748-92294767 Msa0965070:intron 25.0%
! TCAATCAAATCTTAGTCATT+TGG - chr6_3:92294800-92294819 Msa0965070:intron 25.0%
! AAATGTTAAAAGTCTCACAT+CGG - chr6_3:92294967-92294986 Msa0965070:intron 25.0%
!! ATCTGAACATGTTTTATAAG+CGG - chr6_3:92295000-92295019 Msa0965070:intron 25.0%
!! AGAAACAACCATGATTTTTA+AGG - chr6_3:92295065-92295084 Msa0965070:intron 25.0%
! CTATGATACCTTAAAAATCA+TGG + chr6_3:92295076-92295095 None:intergenic 25.0%
!! AAGTTTGTTTGTTTGTTAGT+TGG - chr6_3:92295228-92295247 Msa0965070:CDS 25.0%
! TACAAATTTCAATATGTCAC+GGG + chr6_3:92295263-92295282 None:intergenic 25.0%
! GTACAAATTTCAATATGTCA+CGG + chr6_3:92295264-92295283 None:intergenic 25.0%
!! CATACTTTGAATCTGAAATA+TGG + chr6_3:92295518-92295537 None:intergenic 25.0%
!!! TTGAATTTGGATTTTAGTCA+TGG - chr6_3:92295624-92295643 Msa0965070:five_prime_UTR 25.0%
!!! TGAATTTGGATTTTAGTCAT+GGG - chr6_3:92295625-92295644 Msa0965070:five_prime_UTR 25.0%
! TTAAGAGACAATTCAAAACA+TGG + chr6_3:92293460-92293479 None:intergenic 25.0%
! TGTGTTATGTTCTGTTATTA+TGG - chr6_3:92293735-92293754 Msa0965070:CDS 25.0%
! TTTAGGAGTTAAAAGAATTG+AGG - chr6_3:92293821-92293840 Msa0965070:intron 25.0%
! TTTAAAAAACTGAAACGAAC+CGG - chr6_3:92294105-92294124 Msa0965070:intron 25.0%
!!! TTTTTGAGTTATGGTTCAAT+TGG + chr6_3:92294527-92294546 None:intergenic 25.0%
!!! TCGATTAGATTTTTGAGTTA+TGG + chr6_3:92294536-92294555 None:intergenic 25.0%
! AAAATTGTACATTTGTGTAG+TGG - chr6_3:92294668-92294687 Msa0965070:intron 25.0%
! AAATTGTACATTTGTGTAGT+GGG - chr6_3:92294669-92294688 Msa0965070:intron 25.0%
! TAATCTAAATCAACTTGAGT+TGG - chr6_3:92294748-92294767 Msa0965070:intron 25.0%
! TCAATCAAATCTTAGTCATT+TGG - chr6_3:92294800-92294819 Msa0965070:intron 25.0%
! AAATGTTAAAAGTCTCACAT+CGG - chr6_3:92294967-92294986 Msa0965070:intron 25.0%
!! ATCTGAACATGTTTTATAAG+CGG - chr6_3:92295000-92295019 Msa0965070:intron 25.0%
!! AGAAACAACCATGATTTTTA+AGG - chr6_3:92295065-92295084 Msa0965070:intron 25.0%
! CTATGATACCTTAAAAATCA+TGG + chr6_3:92295076-92295095 None:intergenic 25.0%
!! AAGTTTGTTTGTTTGTTAGT+TGG - chr6_3:92295228-92295247 Msa0965070:CDS 25.0%
! TACAAATTTCAATATGTCAC+GGG + chr6_3:92295263-92295282 None:intergenic 25.0%
! GTACAAATTTCAATATGTCA+CGG + chr6_3:92295264-92295283 None:intergenic 25.0%
!! CATACTTTGAATCTGAAATA+TGG + chr6_3:92295518-92295537 None:intergenic 25.0%
!!! TTGAATTTGGATTTTAGTCA+TGG - chr6_3:92295624-92295643 Msa0965070:five_prime_UTR 25.0%
!!! TGAATTTGGATTTTAGTCAT+GGG - chr6_3:92295625-92295644 Msa0965070:five_prime_UTR 25.0%
TTGGAAGAAGAAATCAACAA+AGG + chr6_3:92293596-92293615 None:intergenic 30.0%
!! TTCAGCTTCTGATTTTTCAA+AGG + chr6_3:92293653-92293672 None:intergenic 30.0%
TTACTGTGAAACAACATTCA+TGG - chr6_3:92293867-92293886 Msa0965070:intron 30.0%
! GAGAGTGAGTATTTTCTATA+TGG - chr6_3:92293905-92293924 Msa0965070:intron 30.0%
! GAACAATTTGCTTACATTTG+TGG - chr6_3:92294057-92294076 Msa0965070:intron 30.0%
AATCAAACCAACTAATGAAC+CGG + chr6_3:92294160-92294179 None:intergenic 30.0%
AATTGTACATTTGTGTAGTG+GGG - chr6_3:92294670-92294689 Msa0965070:intron 30.0%
AGTCTAAAAAATGGAAGAGT+AGG + chr6_3:92294781-92294800 None:intergenic 30.0%
CTAACACAATTACACAAAAC+CGG + chr6_3:92295047-92295066 None:intergenic 30.0%
CGATATCGGAAATCTTATAA+CGG + chr6_3:92295124-92295143 None:intergenic 30.0%
CGTTATAAGATTTCCGATAT+CGG - chr6_3:92295122-92295141 Msa0965070:intron 30.0%
GTTATAAGATTTCCGATATC+GGG - chr6_3:92295123-92295142 Msa0965070:intron 30.0%
!! TTGTACATTTTTCAGTGTGA+TGG - chr6_3:92295278-92295297 Msa0965070:intron 30.0%
AGAATATATATGCAGAGCTA+TGG - chr6_3:92295311-92295330 Msa0965070:intron 30.0%
AATGTTTCCTCTAATCTTGA+AGG - chr6_3:92295357-92295376 Msa0965070:intron 30.0%
ATGCATTTGTGTGAGAAATA+AGG + chr6_3:92295383-92295402 None:intergenic 30.0%
TTGAAAGTAACCATATGGAT+TGG + chr6_3:92295583-92295602 None:intergenic 30.0%
TTGGAAGAAGAAATCAACAA+AGG + chr6_3:92293596-92293615 None:intergenic 30.0%
!! TTCAGCTTCTGATTTTTCAA+AGG + chr6_3:92293653-92293672 None:intergenic 30.0%
TTACTGTGAAACAACATTCA+TGG - chr6_3:92293867-92293886 Msa0965070:intron 30.0%
! GAGAGTGAGTATTTTCTATA+TGG - chr6_3:92293905-92293924 Msa0965070:intron 30.0%
! GAACAATTTGCTTACATTTG+TGG - chr6_3:92294057-92294076 Msa0965070:intron 30.0%
AATCAAACCAACTAATGAAC+CGG + chr6_3:92294160-92294179 None:intergenic 30.0%
AATTGTACATTTGTGTAGTG+GGG - chr6_3:92294670-92294689 Msa0965070:intron 30.0%
AGTCTAAAAAATGGAAGAGT+AGG + chr6_3:92294781-92294800 None:intergenic 30.0%
CTAACACAATTACACAAAAC+CGG + chr6_3:92295047-92295066 None:intergenic 30.0%
CGATATCGGAAATCTTATAA+CGG + chr6_3:92295124-92295143 None:intergenic 30.0%
CGTTATAAGATTTCCGATAT+CGG - chr6_3:92295122-92295141 Msa0965070:intron 30.0%
GTTATAAGATTTCCGATATC+GGG - chr6_3:92295123-92295142 Msa0965070:intron 30.0%
!! TTGTACATTTTTCAGTGTGA+TGG - chr6_3:92295278-92295297 Msa0965070:intron 30.0%
AGAATATATATGCAGAGCTA+TGG - chr6_3:92295311-92295330 Msa0965070:intron 30.0%
AATGTTTCCTCTAATCTTGA+AGG - chr6_3:92295357-92295376 Msa0965070:intron 30.0%
ATGCATTTGTGTGAGAAATA+AGG + chr6_3:92295383-92295402 None:intergenic 30.0%
TTGAAAGTAACCATATGGAT+TGG + chr6_3:92295583-92295602 None:intergenic 30.0%
AGACAATTCAAAACATGGTG+TGG + chr6_3:92293455-92293474 None:intergenic 35.0%
ATAAGGGATTATGTCACATG+TGG + chr6_3:92293524-92293543 None:intergenic 35.0%
!! GTTGGTTTGATTCTTCAGTT+TGG - chr6_3:92294168-92294187 Msa0965070:intron 35.0%
ACCAGATTGAATGTTTGAAC+CGG - chr6_3:92294195-92294214 Msa0965070:intron 35.0%
TATTACTTTCTCCGTCTCAA+TGG - chr6_3:92294283-92294302 Msa0965070:intron 35.0%
! TGTCACTTATTATGAGACGA+AGG - chr6_3:92294320-92294339 Msa0965070:intron 35.0%
CGAAGGAGTTAGAAAAATTG+AGG - chr6_3:92294337-92294356 Msa0965070:intron 35.0%
GCACTAAAGATGCATATCTA+TGG - chr6_3:92294896-92294915 Msa0965070:intron 35.0%
GTAATCTTCACTTTACAAGC+CGG - chr6_3:92295025-92295044 Msa0965070:intron 35.0%
GTGATGTGATCAACACTATT+GGG + chr6_3:92295160-92295179 None:intergenic 35.0%
AGTGATGTGATCAACACTAT+TGG + chr6_3:92295161-92295180 None:intergenic 35.0%
AATAAGGCCTTCAAGATTAG+AGG + chr6_3:92295367-92295386 None:intergenic 35.0%
! TAATAGCACTAACTTGTGTG+AGG + chr6_3:92295434-92295453 None:intergenic 35.0%
TCTCATTATAATCCACACGT+AGG - chr6_3:92295491-92295510 Msa0965070:five_prime_UTR 35.0%
AGACAATTCAAAACATGGTG+TGG + chr6_3:92293455-92293474 None:intergenic 35.0%
ATAAGGGATTATGTCACATG+TGG + chr6_3:92293524-92293543 None:intergenic 35.0%
!! GTTGGTTTGATTCTTCAGTT+TGG - chr6_3:92294168-92294187 Msa0965070:intron 35.0%
ACCAGATTGAATGTTTGAAC+CGG - chr6_3:92294195-92294214 Msa0965070:intron 35.0%
TATTACTTTCTCCGTCTCAA+TGG - chr6_3:92294283-92294302 Msa0965070:intron 35.0%
! TGTCACTTATTATGAGACGA+AGG - chr6_3:92294320-92294339 Msa0965070:intron 35.0%
CGAAGGAGTTAGAAAAATTG+AGG - chr6_3:92294337-92294356 Msa0965070:intron 35.0%
GCACTAAAGATGCATATCTA+TGG - chr6_3:92294896-92294915 Msa0965070:intron 35.0%
GTAATCTTCACTTTACAAGC+CGG - chr6_3:92295025-92295044 Msa0965070:intron 35.0%
GTGATGTGATCAACACTATT+GGG + chr6_3:92295160-92295179 None:intergenic 35.0%
AGTGATGTGATCAACACTAT+TGG + chr6_3:92295161-92295180 None:intergenic 35.0%
AATAAGGCCTTCAAGATTAG+AGG + chr6_3:92295367-92295386 None:intergenic 35.0%
! TAATAGCACTAACTTGTGTG+AGG + chr6_3:92295434-92295453 None:intergenic 35.0%
TCTCATTATAATCCACACGT+AGG - chr6_3:92295491-92295510 Msa0965070:five_prime_UTR 35.0%
CTTCTTCCAAAGCAGTAACA+TGG - chr6_3:92293606-92293625 Msa0965070:CDS 40.0%
TCACATCCATGTTACTGCTT+TGG + chr6_3:92293615-92293634 None:intergenic 40.0%
! TGCATGTTTTCTTCCACTTC+AGG + chr6_3:92293676-92293695 None:intergenic 40.0%
GCACTTTCTTAAGTCTCTTC+AGG - chr6_3:92293693-92293712 Msa0965070:CDS 40.0%
ATTGAAGTAAATCCCCTCAC+AGG + chr6_3:92294083-92294102 None:intergenic 40.0%
AACGAACCGGTCAATTAAAG+CGG - chr6_3:92294118-92294137 Msa0965070:intron 40.0%
! ACCGGTTCAAACATTCAATC+TGG + chr6_3:92294199-92294218 None:intergenic 40.0%
TTGAGATGCTTCCATTGAGA+CGG + chr6_3:92294297-92294316 None:intergenic 40.0%
AATGAAGCCAGGTCACTATT+GGG - chr6_3:92294703-92294722 Msa0965070:intron 40.0%
CAATTAAGATAGGACCGAAG+AGG - chr6_3:92294874-92294893 Msa0965070:intron 40.0%
ATGCATCTTTAGTGCCTCTT+CGG + chr6_3:92294891-92294910 None:intergenic 40.0%
! GCTGGCTTAATGGATCTTAA+CGG + chr6_3:92295102-92295121 None:intergenic 40.0%
!! TCTTATAACGGCTGGCTTAA+TGG + chr6_3:92295112-92295131 None:intergenic 40.0%
ATCGGAAATCTTATAACGGC+TGG + chr6_3:92295120-92295139 None:intergenic 40.0%
ATCAACACTATTGGGCTTTG+TGG + chr6_3:92295152-92295171 None:intergenic 40.0%
GTCACTGTAGTTGATCATCT+TGG - chr6_3:92295333-92295352 Msa0965070:intron 40.0%
! AGACACTGACTGATGCATAT+TGG + chr6_3:92295542-92295561 None:intergenic 40.0%
CTAGGTGAAACCAATCCATA+TGG - chr6_3:92295570-92295589 Msa0965070:five_prime_UTR 40.0%
CTTCTTCCAAAGCAGTAACA+TGG - chr6_3:92293606-92293625 Msa0965070:CDS 40.0%
TCACATCCATGTTACTGCTT+TGG + chr6_3:92293615-92293634 None:intergenic 40.0%
! TGCATGTTTTCTTCCACTTC+AGG + chr6_3:92293676-92293695 None:intergenic 40.0%
GCACTTTCTTAAGTCTCTTC+AGG - chr6_3:92293693-92293712 Msa0965070:CDS 40.0%
ATTGAAGTAAATCCCCTCAC+AGG + chr6_3:92294083-92294102 None:intergenic 40.0%
AACGAACCGGTCAATTAAAG+CGG - chr6_3:92294118-92294137 Msa0965070:intron 40.0%
! ACCGGTTCAAACATTCAATC+TGG + chr6_3:92294199-92294218 None:intergenic 40.0%
TTGAGATGCTTCCATTGAGA+CGG + chr6_3:92294297-92294316 None:intergenic 40.0%
AATGAAGCCAGGTCACTATT+GGG - chr6_3:92294703-92294722 Msa0965070:intron 40.0%
CAATTAAGATAGGACCGAAG+AGG - chr6_3:92294874-92294893 Msa0965070:intron 40.0%
ATGCATCTTTAGTGCCTCTT+CGG + chr6_3:92294891-92294910 None:intergenic 40.0%
! GCTGGCTTAATGGATCTTAA+CGG + chr6_3:92295102-92295121 None:intergenic 40.0%
!! TCTTATAACGGCTGGCTTAA+TGG + chr6_3:92295112-92295131 None:intergenic 40.0%
ATCGGAAATCTTATAACGGC+TGG + chr6_3:92295120-92295139 None:intergenic 40.0%
ATCAACACTATTGGGCTTTG+TGG + chr6_3:92295152-92295171 None:intergenic 40.0%
GTCACTGTAGTTGATCATCT+TGG - chr6_3:92295333-92295352 Msa0965070:intron 40.0%
! AGACACTGACTGATGCATAT+TGG + chr6_3:92295542-92295561 None:intergenic 40.0%
CTAGGTGAAACCAATCCATA+TGG - chr6_3:92295570-92295589 Msa0965070:five_prime_UTR 40.0%
ATCAGAAGCTGAACCTGAAG+TGG - chr6_3:92293660-92293679 Msa0965070:CDS 45.0%
CTTACATTTGTGGCCTGTGA+GGG - chr6_3:92294067-92294086 Msa0965070:intron 45.0%
TTACATTTGTGGCCTGTGAG+GGG - chr6_3:92294068-92294087 Msa0965070:intron 45.0%
GCTGAACCGCTTTAATTGAC+CGG + chr6_3:92294127-92294146 None:intergenic 45.0%
AAACCAACTAATGAACCGGC+TGG + chr6_3:92294156-92294175 None:intergenic 45.0%
TGTTTGAACCGGTTGAGTCA+CGG - chr6_3:92294206-92294225 Msa0965070:intron 45.0%
CATAGGTCATGGAGCTTTCA+AGG + chr6_3:92294596-92294615 None:intergenic 45.0%
CTTGAAAGCTCCATGACCTA+TGG - chr6_3:92294594-92294613 Msa0965070:intron 45.0%
CAATAGTGACCTGGCTTCAT+TGG + chr6_3:92294704-92294723 None:intergenic 45.0%
CAATGAAGCCAGGTCACTAT+TGG - chr6_3:92294702-92294721 Msa0965070:intron 45.0%
ATGAAGCCAGGTCACTATTG+GGG - chr6_3:92294704-92294723 Msa0965070:intron 45.0%
! GGCTTAATGGATCTTAACGG+AGG + chr6_3:92295099-92295118 None:intergenic 45.0%
! AACACTATTGGGCTTTGTGG+TGG + chr6_3:92295149-92295168 None:intergenic 45.0%
! CACTAACTTGTGTGAGGCAT+TGG + chr6_3:92295428-92295447 None:intergenic 45.0%
CTGAAATATGGTCCTACGTG+TGG + chr6_3:92295506-92295525 None:intergenic 45.0%
AGTCCACATTCACATAGTCG+TGG - chr6_3:92295648-92295667 Msa0965070:five_prime_UTR 45.0%
ATGCCACGACTATGTGAATG+TGG + chr6_3:92295654-92295673 None:intergenic 45.0%
CATTCACATAGTCGTGGCAT+CGG - chr6_3:92295654-92295673 Msa0965070:five_prime_UTR 45.0%
ATCAGAAGCTGAACCTGAAG+TGG - chr6_3:92293660-92293679 Msa0965070:CDS 45.0%
CTTACATTTGTGGCCTGTGA+GGG - chr6_3:92294067-92294086 Msa0965070:intron 45.0%
TTACATTTGTGGCCTGTGAG+GGG - chr6_3:92294068-92294087 Msa0965070:intron 45.0%
GCTGAACCGCTTTAATTGAC+CGG + chr6_3:92294127-92294146 None:intergenic 45.0%
AAACCAACTAATGAACCGGC+TGG + chr6_3:92294156-92294175 None:intergenic 45.0%
TGTTTGAACCGGTTGAGTCA+CGG - chr6_3:92294206-92294225 Msa0965070:intron 45.0%
CATAGGTCATGGAGCTTTCA+AGG + chr6_3:92294596-92294615 None:intergenic 45.0%
CTTGAAAGCTCCATGACCTA+TGG - chr6_3:92294594-92294613 Msa0965070:intron 45.0%
CAATAGTGACCTGGCTTCAT+TGG + chr6_3:92294704-92294723 None:intergenic 45.0%
CAATGAAGCCAGGTCACTAT+TGG - chr6_3:92294702-92294721 Msa0965070:intron 45.0%
ATGAAGCCAGGTCACTATTG+GGG - chr6_3:92294704-92294723 Msa0965070:intron 45.0%
! GGCTTAATGGATCTTAACGG+AGG + chr6_3:92295099-92295118 None:intergenic 45.0%
! AACACTATTGGGCTTTGTGG+TGG + chr6_3:92295149-92295168 None:intergenic 45.0%
! CACTAACTTGTGTGAGGCAT+TGG + chr6_3:92295428-92295447 None:intergenic 45.0%
CTGAAATATGGTCCTACGTG+TGG + chr6_3:92295506-92295525 None:intergenic 45.0%
AGTCCACATTCACATAGTCG+TGG - chr6_3:92295648-92295667 Msa0965070:five_prime_UTR 45.0%
ATGCCACGACTATGTGAATG+TGG + chr6_3:92295654-92295673 None:intergenic 45.0%
CATTCACATAGTCGTGGCAT+CGG - chr6_3:92295654-92295673 Msa0965070:five_prime_UTR 45.0%
GCTTACATTTGTGGCCTGTG+AGG - chr6_3:92294066-92294085 Msa0965070:intron 50.0%
! CTAATGAACCGGCTGGTTCT+TGG + chr6_3:92294149-92294168 None:intergenic 50.0%
! GAACCAGCCGGTTCATTAGT+TGG - chr6_3:92294150-92294169 Msa0965070:intron 50.0%
TGAACCAACCGTGACTCAAC+CGG + chr6_3:92294217-92294236 None:intergenic 50.0%
AAAGCTCCATGACCTATGGC+AGG - chr6_3:92294598-92294617 Msa0965070:intron 50.0%
ATCTGTCCTGCCATAGGTCA+TGG + chr6_3:92294607-92294626 None:intergenic 50.0%
GACCTATGGCAGGACAGATT+TGG - chr6_3:92294608-92294627 Msa0965070:intron 50.0%
GTCCAAATCTGTCCTGCCAT+AGG + chr6_3:92294613-92294632 None:intergenic 50.0%
TGAAGCCAGGTCACTATTGG+GGG - chr6_3:92294705-92294724 Msa0965070:intron 50.0%
GTCAGTGTCTGTCTGACACT+AGG - chr6_3:92295552-92295571 Msa0965070:five_prime_UTR 50.0%
GCTTACATTTGTGGCCTGTG+AGG - chr6_3:92294066-92294085 Msa0965070:intron 50.0%
! CTAATGAACCGGCTGGTTCT+TGG + chr6_3:92294149-92294168 None:intergenic 50.0%
! GAACCAGCCGGTTCATTAGT+TGG - chr6_3:92294150-92294169 Msa0965070:intron 50.0%
TGAACCAACCGTGACTCAAC+CGG + chr6_3:92294217-92294236 None:intergenic 50.0%
AAAGCTCCATGACCTATGGC+AGG - chr6_3:92294598-92294617 Msa0965070:intron 50.0%
ATCTGTCCTGCCATAGGTCA+TGG + chr6_3:92294607-92294626 None:intergenic 50.0%
GACCTATGGCAGGACAGATT+TGG - chr6_3:92294608-92294627 Msa0965070:intron 50.0%
GTCCAAATCTGTCCTGCCAT+AGG + chr6_3:92294613-92294632 None:intergenic 50.0%
TGAAGCCAGGTCACTATTGG+GGG - chr6_3:92294705-92294724 Msa0965070:intron 50.0%
GTCAGTGTCTGTCTGACACT+AGG - chr6_3:92295552-92295571 Msa0965070:five_prime_UTR 50.0%
TGAACCGGTTGAGTCACGGT+TGG - chr6_3:92294210-92294229 Msa0965070:intron 55.0%
GACCACTCACCAATGAAGCC+AGG - chr6_3:92294692-92294711 Msa0965070:intron 55.0%
GACCTGGCTTCATTGGTGAG+TGG + chr6_3:92294697-92294716 None:intergenic 55.0%
CAATGCCCCCAATAGTGACC+TGG + chr6_3:92294713-92294732 None:intergenic 55.0%
! CAGGTCACTATTGGGGGCAT+TGG - chr6_3:92294711-92294730 Msa0965070:intron 55.0%
GCTTTGTGGTGGCCCGATAT+CGG + chr6_3:92295138-92295157 None:intergenic 55.0%
TGAACCGGTTGAGTCACGGT+TGG - chr6_3:92294210-92294229 Msa0965070:intron 55.0%
GACCACTCACCAATGAAGCC+AGG - chr6_3:92294692-92294711 Msa0965070:intron 55.0%
GACCTGGCTTCATTGGTGAG+TGG + chr6_3:92294697-92294716 None:intergenic 55.0%
CAATGCCCCCAATAGTGACC+TGG + chr6_3:92294713-92294732 None:intergenic 55.0%
! CAGGTCACTATTGGGGGCAT+TGG - chr6_3:92294711-92294730 Msa0965070:intron 55.0%
GCTTTGTGGTGGCCCGATAT+CGG + chr6_3:92295138-92295157 None:intergenic 55.0%
CGGTTCAGCCAAGAACCAGC+CGG - chr6_3:92294138-92294157 Msa0965070:intron 60.0%
CGGTTCAGCCAAGAACCAGC+CGG - chr6_3:92294138-92294157 Msa0965070:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 92293406 92295690 92293406 ID=Msa0965070;Name=Msa0965070
chr6_3 mRNA 92293406 92295690 92293406 ID=Msa0965070-mRNA-1;Parent=Msa0965070;Name=Msa0965070-mRNA-1;_AED=0.17;_eAED=0.17;_QI=219|1|1|1|1|1|3|178|130
chr6_3 exon 92293406 92293803 92293406 ID=Msa0965070-mRNA-1:exon:16780;Parent=Msa0965070-mRNA-1
chr6_3 exon 92295188 92295270 92295188 ID=Msa0965070-mRNA-1:exon:16779;Parent=Msa0965070-mRNA-1
chr6_3 exon 92295382 92295690 92295382 ID=Msa0965070-mRNA-1:exon:16778;Parent=Msa0965070-mRNA-1
chr6_3 five_prime_UTR 92295472 92295690 92295472 ID=Msa0965070-mRNA-1:five_prime_utr;Parent=Msa0965070-mRNA-1
chr6_3 CDS 92295382 92295471 92295382 ID=Msa0965070-mRNA-1:cds;Parent=Msa0965070-mRNA-1
chr6_3 CDS 92295188 92295270 92295188 ID=Msa0965070-mRNA-1:cds;Parent=Msa0965070-mRNA-1
chr6_3 CDS 92293584 92293803 92293584 ID=Msa0965070-mRNA-1:cds;Parent=Msa0965070-mRNA-1
chr6_3 three_prime_UTR 92293406 92293583 92293406 ID=Msa0965070-mRNA-1:three_prime_utr;Parent=Msa0965070-mRNA-1
Gene Sequence

>Msa0965070

ATGGATGTGAATTTTAAGTCACTTTCCTTTGAAAAATCAGAAGCTGAACCTGAAGTGGAAGAAAACATGCACTTTCTTAAGTCTCTTCAGGAGTTAAAAGAATTGAGGTCTCAGCTTCACTATGCTGCAGATTACTGTGAAACAACATTCATGGAAAGTGAAAAGAAGAGAGATGTGATGGATGACACAAAAGAATATATATGCAGAGCTATGGTCACTGTAGTTGATCATCTTGGAAATGTTTCCTCTAATCTTGAAGGCCTTATTTCTCACACAAATGCATTTTCTGATGCTGAGATACGAATCCAATGCCTCACACAAGTTAGTGCTATTACTTGCACAAATGCACTTATGTGCATAATTATATGTTATCTCATTATAATCCACACGTAG
Protein sequence

>Msa0965070

MDVNFKSLSFEKSEAEPEVEENMHFLKSLQELKELRSQLHYAADYCETTFMESEKKRDVMDDTKEYICRAMVTVVDHLGNVSSNLEGLISHTNAFSDAEIRIQCLTQVSAITCTNALMCIIICYLIIIHT*