AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0103000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0103000 MtrunA17_Chr1g0156911 97.778 90 2 0 1 90 46 135 1.43e-59 185
Msa0103000 MtrunA17_Chr1g0156901 84.444 90 14 0 1 90 59 148 6.05e-52 166
Msa0103000 MtrunA17_Chr1g0156891 76.923 91 20 1 1 90 52 142 1.32e-43 144
Msa0103000 MtrunA17_Chr3g0142941 76.087 92 21 1 1 92 56 146 1.12e-42 142
Msa0103000 MtrunA17_Chr3g0142921 79.070 86 17 1 1 85 45 130 2.37e-42 141
Msa0103000 MtrunA17_Chr1g0156921 76.404 89 21 0 2 90 52 140 2.77e-42 141
Msa0103000 MtrunA17_Chr1g0156931 64.444 90 32 0 1 90 50 139 9.00e-39 132
Msa0103000 MtrunA17_Chr8g0371491 65.116 86 30 0 1 86 45 130 4.81e-37 127
Msa0103000 MtrunA17_Chr5g0405821 67.816 87 26 2 1 85 52 138 4.74e-33 117
Msa0103000 MtrunA17_Chr5g0405831 59.341 91 36 1 1 90 52 142 1.48e-31 113
Msa0103000 MtrunA17_Chr8g0371481 60.465 86 34 0 1 86 50 135 6.50e-31 111
Msa0103000 MtrunA17_Chr1g0157001 53.261 92 43 0 1 92 45 136 7.16e-31 111
Msa0103000 MtrunA17_Chr8g0371471 58.140 86 36 0 1 86 50 135 2.41e-29 107
Msa0103000 MtrunA17_Chr5g0438641 56.977 86 37 0 1 86 41 126 4.54e-29 106
Msa0103000 MtrunA17_Chr3g0120861 54.651 86 39 0 1 86 47 132 4.53e-27 101
Msa0103000 MtrunA17_Chr5g0438621 53.488 86 40 0 1 86 39 124 1.61e-26 99.8
Msa0103000 MtrunA17_Chr4g0046151 57.831 83 35 0 1 83 45 127 1.69e-26 99.8
Msa0103000 MtrunA17_Chr5g0438631 55.814 86 38 0 1 86 37 122 3.74e-26 98.6
Msa0103000 MtrunA17_Chr2g0283191 54.839 93 39 2 1 90 47 139 4.33e-25 95.9
Msa0103000 MtrunA17_Chr3g0105401 52.326 86 41 0 1 86 37 122 8.04e-25 95.1
Msa0103000 MtrunA17_Chr2g0320671 48.889 90 46 0 1 90 39 128 1.20e-22 89.4
Msa0103000 MtrunA17_Chr5g0432101 51.807 83 40 0 1 83 42 124 3.01e-22 88.2
Msa0103000 MtrunA17_Chr4g0027521 49.412 85 43 0 1 85 36 120 1.43e-21 86.3
Msa0103000 MtrunA17_Chr4g0027561 48.810 84 43 0 1 84 39 122 5.21e-21 80.9
Msa0103000 MtrunA17_Chr8g0341001 43.333 90 51 0 1 90 41 130 6.45e-21 84.7
Msa0103000 MtrunA17_Chr2g0330781 44.444 90 50 0 1 90 40 129 7.45e-21 84.3
Msa0103000 MtrunA17_Chr6g0469511 47.059 85 45 0 1 85 39 123 1.94e-20 79.3
Msa0103000 MtrunA17_Chr6g0463261 83.333 42 7 0 44 85 3 44 3.33e-19 75.9
Msa0103000 MtrunA17_Chr7g0229591 46.512 86 46 0 1 86 39 124 4.18e-19 77.8
Msa0103000 MtrunA17_Chr5g0437421 50.000 68 34 0 1 68 41 108 8.31e-19 79.0
Msa0103000 MtrunA17_Chr2g0284941 48.052 77 37 2 2 78 43 116 8.75e-18 76.3
Msa0103000 MtrunA17_Chr8g0380031 48.052 77 37 2 2 78 94 167 1.05e-17 76.3
Msa0103000 MtrunA17_Chr2g0284951 44.944 89 41 3 2 90 43 123 1.27e-17 75.9
Msa0103000 MtrunA17_Chr7g0275021 51.282 78 37 1 1 78 43 119 3.49e-17 74.3
Msa0103000 MtrunA17_Chr2g0284991 45.783 83 42 2 2 84 43 122 4.01e-17 74.3
Msa0103000 MtrunA17_Chr5g0420811 42.857 91 50 2 1 90 42 131 5.93e-17 73.6
Msa0103000 MtrunA17_Chr2g0284921 44.828 87 46 1 2 88 44 128 1.26e-16 72.8
Msa0103000 MtrunA17_Chr1g0189921 49.351 77 38 1 2 78 41 116 1.40e-16 69.7
Msa0103000 MtrunA17_Chr2g0285061 46.154 78 39 2 1 78 41 115 5.32e-16 71.2
Msa0103000 MtrunA17_Chr2g0321991 46.835 79 40 2 1 78 42 119 5.46e-16 67.8
Msa0103000 MtrunA17_Chr8g0374101 43.956 91 49 1 1 89 46 136 5.68e-16 71.2
Msa0103000 MtrunA17_Chr5g0396511 42.308 78 44 1 1 78 55 131 7.11e-16 70.9
Msa0103000 MtrunA17_Chr3g0086641 47.436 78 38 2 1 78 47 121 1.82e-15 69.7
Msa0103000 MtrunA17_Chr1g0182811 47.436 78 41 0 1 78 42 119 5.92e-15 68.2
Msa0103000 MtrunA17_Chr2g0285001 46.154 78 38 3 2 78 43 117 8.23e-15 67.8
Msa0103000 MtrunA17_Chr2g0294301 44.318 88 46 2 1 88 44 128 1.66e-14 67.0
Msa0103000 MtrunA17_Chr6g0457921 48.101 79 39 2 1 78 38 115 3.08e-14 66.2
Msa0103000 MtrunA17_Chr1g0189901 50.649 77 37 1 2 78 41 116 3.15e-14 66.2
Msa0103000 MtrunA17_Chr1g0189931 49.351 77 38 1 2 78 42 117 6.42e-14 65.5
Msa0103000 MtrunA17_Chr1g0189891 49.367 79 38 2 1 78 38 115 9.22e-14 65.1
Msa0103000 MtrunA17_Chr8g0380041 44.872 78 39 2 1 78 40 113 1.01e-13 64.7
Msa0103000 MtrunA17_Chr7g0275011 47.436 78 40 1 1 78 43 119 2.00e-13 63.9
Msa0103000 MtrunA17_Chr6g0455661 49.383 81 37 3 1 81 40 116 2.16e-13 63.9
Msa0103000 MtrunA17_Chr8g0370981 45.122 82 41 2 1 78 53 134 5.13e-13 62.8
Msa0103000 MtrunA17_Chr5g0434621 47.436 78 39 2 2 78 35 111 5.40e-13 62.8
Msa0103000 MtrunA17_Chr7g0275001 47.436 78 40 1 1 78 34 110 9.72e-13 62.0
Msa0103000 MtrunA17_Chr3g0099161 45.238 84 40 2 1 78 101 184 1.03e-12 62.0
Msa0103000 MtrunA17_Chr3g0099151 44.048 84 41 2 1 78 96 179 1.13e-12 62.0
Msa0103000 MtrunA17_Chr3g0077581 42.500 80 39 3 1 78 45 119 2.68e-12 60.8
Msa0103000 MtrunA17_Chr3g0077571 46.154 78 38 3 1 78 39 112 3.17e-12 60.5
Msa0103000 MtrunA17_Chr4g0017431 46.154 78 40 2 1 78 40 115 3.36e-12 60.5
Msa0103000 MtrunA17_Chr7g0274991 46.154 78 41 1 1 78 42 118 4.24e-12 60.1
Msa0103000 MtrunA17_Chr3g0092131 44.304 79 41 3 2 78 33 110 4.65e-12 60.1
Msa0103000 MtrunA17_Chr4g0008851 46.154 78 41 1 1 78 41 117 7.57e-12 59.7
Msa0103000 MtrunA17_Chr3g0085881 41.667 84 45 3 2 85 43 122 9.11e-12 59.3
Msa0103000 MtrunA17_Chr3g0099231 46.512 86 35 4 1 78 37 119 1.84e-11 58.5
Msa0103000 MtrunA17_Chr3g0085231 45.570 79 40 3 1 78 40 116 2.11e-11 58.2
Msa0103000 MtrunA17_Chr4g0060811 43.038 79 42 3 2 78 37 114 8.65e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0103000 AT3G43550.1 66.279 86 29 0 1 86 39 124 1.04e-37 127
Msa0103000 AT3G43570.1 65.116 86 30 0 1 86 39 124 3.25e-37 127
Msa0103000 AT1G59406.1 64.444 90 32 0 1 90 39 128 3.41e-37 128
Msa0103000 AT1G59030.1 64.444 90 32 0 1 90 39 128 3.41e-37 128
Msa0103000 AT1G58725.1 64.444 90 32 0 1 90 39 128 3.41e-37 128
Msa0103000 AT1G58520.3 63.953 86 31 0 1 86 39 124 1.17e-34 121
Msa0103000 AT1G58525.1 63.953 86 31 0 1 86 39 124 1.17e-34 121
Msa0103000 AT5G42170.1 61.628 86 33 0 1 86 59 144 1.42e-34 121
Msa0103000 AT1G75880.1 63.636 88 32 0 1 88 61 148 1.55e-34 121
Msa0103000 AT1G75900.1 60.215 93 37 0 1 93 52 144 1.59e-34 121
Msa0103000 AT3G14820.1 65.116 86 30 0 1 86 41 126 5.10e-34 119
Msa0103000 AT1G75880.2 63.953 86 31 0 1 86 61 146 6.04e-34 120
Msa0103000 AT1G20132.1 62.069 87 33 0 1 87 70 156 2.18e-31 113
Msa0103000 AT1G75890.1 55.435 92 40 1 1 91 56 147 1.05e-29 108
Msa0103000 AT1G75890.2 56.818 88 37 1 1 87 56 143 5.79e-29 106
Msa0103000 AT1G75890.3 56.818 88 37 1 1 87 85 172 7.39e-29 107
Msa0103000 AT1G20130.2 55.056 89 40 0 2 90 194 282 1.87e-28 107
Msa0103000 AT1G20130.1 54.444 90 41 0 1 90 213 302 1.92e-28 107
Msa0103000 AT5G63170.1 53.846 91 42 0 1 91 37 127 2.24e-28 104
Msa0103000 AT5G45960.1 55.814 86 38 0 1 86 56 141 2.09e-27 102
Msa0103000 AT1G73610.1 53.846 91 42 0 1 91 44 134 3.71e-27 101
Msa0103000 AT2G04570.1 53.488 86 40 0 1 86 37 122 7.10e-26 98.2
Msa0103000 AT1G20135.1 51.613 93 45 0 1 93 38 130 7.51e-26 97.8
Msa0103000 AT2G40250.1 52.222 90 43 0 1 90 45 134 1.76e-25 97.4
Msa0103000 AT1G75920.2 54.651 86 39 0 1 86 38 123 2.20e-25 97.1
Msa0103000 AT1G20120.1 51.111 90 44 0 1 90 87 176 3.77e-25 97.1
Msa0103000 AT1G06990.1 52.941 85 40 0 1 85 46 130 3.81e-24 94.0
Msa0103000 AT1G23500.1 53.846 91 42 0 1 91 44 134 1.22e-23 92.4
Msa0103000 AT2G42990.1 48.837 86 44 0 1 86 37 122 3.46e-22 88.6
Msa0103000 AT1G75910.1 53.488 86 40 0 1 86 37 122 5.13e-22 87.8
Msa0103000 AT5G03810.1 48.810 84 43 0 1 84 39 122 7.08e-22 87.4
Msa0103000 AT1G75920.4 53.947 76 35 0 11 86 20 95 9.92e-22 86.7
Msa0103000 AT1G75920.1 46.535 101 39 1 1 86 33 133 1.00e-21 87.0
Msa0103000 AT2G31540.1 52.941 85 39 1 1 84 44 128 1.12e-21 87.0
Msa0103000 AT4G26790.1 48.837 86 44 0 1 86 38 123 1.20e-21 87.0
Msa0103000 AT4G26790.2 48.837 86 44 0 1 86 38 123 1.20e-21 87.0
Msa0103000 AT1G58430.1 53.488 86 37 2 1 84 44 128 2.90e-21 85.9
Msa0103000 AT2G24560.2 50.588 85 41 1 1 84 43 127 4.55e-21 85.1
Msa0103000 AT2G24560.1 50.588 85 41 1 1 84 43 127 6.50e-21 85.1
Msa0103000 AT3G16370.1 44.444 90 50 0 1 90 39 128 9.69e-21 84.3
Msa0103000 AT5G22810.1 45.556 90 49 0 1 90 43 132 2.73e-20 83.2
Msa0103000 AT2G30310.1 50.588 85 41 1 1 84 43 127 2.84e-20 83.2
Msa0103000 AT5G03820.1 48.750 80 41 0 1 80 42 121 5.35e-19 79.7
Msa0103000 AT5G03820.2 48.750 80 41 0 1 80 70 149 6.78e-19 79.7
Msa0103000 AT2G30220.1 50.000 84 41 1 2 84 43 126 1.09e-18 79.0
Msa0103000 AT1G75930.1 50.000 86 42 1 1 85 38 123 4.86e-18 77.0
Msa0103000 AT5G45950.1 45.349 86 46 1 1 86 49 133 3.58e-17 74.7
Msa0103000 AT3G53100.1 44.048 84 47 0 1 84 38 121 4.83e-17 74.3
Msa0103000 AT3G50400.1 47.500 80 40 1 1 78 43 122 5.63e-17 74.3
Msa0103000 AT2G03980.2 41.748 103 49 3 1 93 52 153 7.24e-17 73.9
Msa0103000 AT2G03980.3 41.748 103 49 3 1 93 52 153 7.24e-17 73.9
Msa0103000 AT2G03980.1 41.748 103 49 3 1 93 52 153 7.24e-17 73.9
Msa0103000 AT2G03980.4 41.748 103 49 3 1 93 52 153 7.24e-17 73.9
Msa0103000 AT2G04020.1 47.126 87 44 2 1 86 52 137 1.12e-16 72.4
Msa0103000 AT2G04020.2 47.126 87 44 2 1 86 52 137 2.38e-16 72.4
Msa0103000 AT2G23540.1 42.857 91 50 1 1 89 57 147 3.03e-16 72.4
Msa0103000 AT2G19010.1 45.238 84 43 2 2 85 40 120 1.83e-15 70.1
Msa0103000 AT2G19010.2 45.238 84 43 2 2 85 70 150 2.34e-15 69.7
Msa0103000 AT4G16230.1 43.023 86 47 1 1 86 39 122 2.67e-15 68.6
Msa0103000 AT1G29660.1 44.872 78 39 2 1 78 41 114 7.40e-15 68.6
Msa0103000 AT1G29670.2 46.154 78 38 2 1 78 109 182 1.29e-14 67.8
Msa0103000 AT1G29670.1 43.529 85 44 2 1 85 41 121 1.36e-14 67.8
Msa0103000 AT4G18970.1 42.353 85 46 2 1 85 37 118 1.90e-14 67.4
Msa0103000 AT5G41890.1 45.000 80 42 1 1 78 40 119 2.17e-14 67.0
Msa0103000 AT4G18970.2 42.353 85 46 2 1 85 37 118 2.36e-14 67.0
Msa0103000 AT5G08460.1 44.872 78 42 1 1 78 58 134 2.72e-14 67.0
Msa0103000 AT4G10950.2 41.026 78 45 1 1 78 80 156 6.25e-14 65.9
Msa0103000 AT2G19060.1 44.706 85 44 2 1 85 39 120 8.21e-14 65.5
Msa0103000 AT4G28780.1 47.436 78 40 1 1 78 40 116 9.76e-14 65.1
Msa0103000 AT5G18430.1 48.718 78 40 0 1 78 37 114 1.08e-13 65.1
Msa0103000 AT2G19050.2 41.176 85 47 2 1 85 40 121 6.90e-13 62.4
Msa0103000 AT5G33370.2 47.436 78 40 1 1 78 39 115 7.24e-13 62.8
Msa0103000 AT5G33370.1 47.436 78 40 1 1 78 39 115 9.38e-13 62.4
Msa0103000 AT2G19050.1 41.176 85 47 2 1 85 40 121 1.00e-12 62.4
Msa0103000 AT1G33811.1 39.744 78 44 2 1 78 43 117 4.54e-12 60.5
Msa0103000 AT5G37690.1 43.590 78 42 2 2 78 37 113 5.01e-12 60.5
Msa0103000 AT3G04290.1 44.872 78 42 1 1 78 38 114 6.01e-12 60.1
Msa0103000 AT1G74460.1 41.772 79 43 3 2 78 33 110 7.24e-12 60.1
Msa0103000 AT4G10950.1 41.026 78 41 2 1 78 80 152 2.06e-11 58.5
Msa0103000 AT5G55050.1 45.238 84 40 2 1 78 48 131 3.86e-11 57.8
Msa0103000 AT4G16220.1 41.667 84 45 2 1 84 39 118 3.92e-11 57.4

Find 63 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATATCTTCCAGCATATT+TGG 0.150743 1_3:-9928375 Msa0103000:CDS
ACTTGGAAAATTCTGCTTAA+AGG 0.193594 1_3:+9927063 None:intergenic
ATTCTTTGAATAATTCTATC+TGG 0.217150 1_3:+9928026 None:intergenic
AAGTAGCAGGATTGTTTATA+TGG 0.252807 1_3:-9927044 Msa0103000:three_prime_UTR
TTTCCAGCAGTGTTTGTATT+TGG 0.284628 1_3:-9928662 Msa0103000:intron
TTACTATCAGTTGAAGAATT+AGG 0.313281 1_3:-9928404 Msa0103000:intron
AGCTGCCTGCTTATTATATA+AGG 0.316492 1_3:+9928063 None:intergenic
TACGGGTCTTAAGAATCCTT+TGG 0.326151 1_3:+9929262 None:intergenic
ACCGGTGTTAACTTTGCTTC+CGG 0.332637 1_3:-9928323 Msa0103000:CDS
AATTCACTAGGCTGAAGATT+TGG 0.343354 1_3:+9928350 None:intergenic
GGAGATTCAATCATGGATAC+TGG 0.365221 1_3:-9928641 Msa0103000:exon
TTACAACGATATCAAGTATA+GGG 0.370204 1_3:+9927011 None:intergenic
CAGCCTAGTGAATTGGTTAC+CGG 0.373888 1_3:-9928341 Msa0103000:CDS
TATAAGCTGAGTCTACTAAA+AGG 0.388730 1_3:+9927846 None:intergenic
CCTTGAAAATCTTTGCCATA+TGG 0.389112 1_3:+9928575 None:intergenic
AAATCTTCAGCCTAGTGAAT+TGG 0.390537 1_3:-9928348 Msa0103000:CDS
CTAGAAAGAAAGAAACAAAA+AGG 0.402491 1_3:-9928134 Msa0103000:three_prime_UTR
AACTTTGCTTCCGGTGGTGC+AGG 0.405817 1_3:-9928314 Msa0103000:CDS
ACGGGTCTTAAGAATCCTTT+GGG 0.414320 1_3:+9929263 None:intergenic
TAGCACAAAATAAATACTAT+TGG 0.418137 1_3:+9927949 None:intergenic
TTTACAACGATATCAAGTAT+AGG 0.431490 1_3:+9927010 None:intergenic
TTCATACACACAACTCTCTC+TGG 0.432556 1_3:-9929325 Msa0103000:five_prime_UTR
GTATTCAATGTACCACCAAT+TGG 0.435799 1_3:-9927196 Msa0103000:three_prime_UTR
ATATAAACAATCCTGCTACT+TGG 0.437775 1_3:+9927046 None:intergenic
AAGGTTCCAGCAGATCTTAT+AGG 0.461354 1_3:-9928521 Msa0103000:intron
TGGATAATTAATTCAATTCA+CGG 0.466344 1_3:-9926885 Msa0103000:three_prime_UTR
TTTCAAGGAGGAATTCCAAC+TGG 0.466996 1_3:-9928560 Msa0103000:CDS
TTCTTTGAATAATTCTATCT+GGG 0.473576 1_3:+9928027 None:intergenic
ATCTGAGAAGATTAAAATCA+AGG 0.478036 1_3:-9929292 Msa0103000:five_prime_UTR
TGAAACGGTACGCACCCAAT+TGG 0.481254 1_3:+9927181 None:intergenic
CAGTGCAAATTCCCTCCATA+TGG 0.481728 1_3:-9928590 Msa0103000:CDS
ATACTGGAAATAACAACAAC+AGG 0.490434 1_3:-9928625 Msa0103000:CDS
ATAGTACCTATAAGATCTGC+TGG 0.491106 1_3:+9928515 None:intergenic
TATTCAATGTACCACCAATT+GGG 0.499652 1_3:-9927195 Msa0103000:three_prime_UTR
TGTATTTGGAGATTCAATCA+TGG 0.510260 1_3:-9928648 Msa0103000:exon
CAACTAGGTGCAAGGAGGAT+AGG 0.513503 1_3:-9927220 Msa0103000:three_prime_UTR
TTAGAGATTGATTTGATCGA+TGG 0.513564 1_3:+9928241 None:intergenic
CCAGCCACTGTTCTCTGAAA+CGG 0.517712 1_3:+9927166 None:intergenic
CTCAGCTTATAATTTCTACA+AGG 0.522865 1_3:-9927834 Msa0103000:intron
AACGGTACGCACCCAATTGG+TGG 0.534120 1_3:+9927184 None:intergenic
CCATTGCTAAATCTTCCAGT+TGG 0.534561 1_3:+9928545 None:intergenic
AATTTGCACTGAGTTGGTGT+TGG 0.536542 1_3:+9928602 None:intergenic
TGTTTCCTTATATAATAAGC+AGG 0.538754 1_3:-9928068 Msa0103000:three_prime_UTR
TGAAAATCTTTGCCATATGG+AGG 0.540087 1_3:+9928578 None:intergenic
AGATTTGGATCCAAATATGC+TGG 0.543536 1_3:+9928365 None:intergenic
GAATATATTGTGAAGCTGAA+AGG 0.555476 1_3:-9928007 Msa0103000:three_prime_UTR
AGGATCATATCCTGCACCAC+CGG 0.561279 1_3:+9928304 None:intergenic
TGGAAGATTTAGCAATGGAA+AGG 0.566706 1_3:-9928540 Msa0103000:CDS
ACTAAAAGGTCAGAGTATGA+AGG 0.568719 1_3:+9927860 None:intergenic
GAAAATCTTTGCCATATGGA+GGG 0.573041 1_3:+9928579 None:intergenic
CCGTTTCAGAGAACAGTGGC+TGG 0.574955 1_3:-9927166 Msa0103000:three_prime_UTR
GGAGGGAATTTGCACTGAGT+TGG 0.578300 1_3:+9928596 None:intergenic
GTGAAGCTGAAAGGACTAGT+TGG 0.592305 1_3:-9927998 Msa0103000:three_prime_UTR
AAATGTATCAACTAGGTGCA+AGG 0.593045 1_3:-9927228 Msa0103000:three_prime_UTR
GGTGTTAACTTTGCTTCCGG+TGG 0.610616 1_3:-9928320 Msa0103000:CDS
TGAAAGGAAATGTATCAACT+AGG 0.611457 1_3:-9927235 Msa0103000:intron
CCAACTGGAAGATTTAGCAA+TGG 0.628801 1_3:-9928545 Msa0103000:CDS
ATATGGTTAATTCATCTCAG+TGG 0.634893 1_3:-9926905 Msa0103000:three_prime_UTR
ACACCGGTAACCAATTCACT+AGG 0.656294 1_3:+9928338 None:intergenic
TTTCAGAGAACAGTGGCTGG+AGG 0.665786 1_3:-9927163 Msa0103000:three_prime_UTR
ACCGGAAGCAAAGTTAACAC+CGG 0.679800 1_3:+9928322 None:intergenic
TGTATCAACTAGGTGCAAGG+AGG 0.714837 1_3:-9927225 Msa0103000:three_prime_UTR
CGTACCGTTTCAGAGAACAG+TGG 0.763484 1_3:-9927170 Msa0103000:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTACTATCATCAAT+TGG + chr1_3:9927983-9928002 None:intergenic 15.0%
!! TGAAATCAAAAATATTTACA+AGG + chr1_3:9928418-9928437 None:intergenic 15.0%
!!! ATTTAGAATTACCATATTTT+TGG + chr1_3:9929217-9929236 None:intergenic 15.0%
!!! AGTAACAAATTTTTTCCTTA+CGG + chr1_3:9926957-9926976 None:intergenic 20.0%
!!! TATATAGTTTTGGTTAATAC+TGG + chr1_3:9927403-9927422 None:intergenic 20.0%
!! GTATTAACCAAAACTATATA+TGG - chr1_3:9927403-9927422 Msa0103000:intron 20.0%
!!! AAAAGAACCATATATAGTTT+TGG + chr1_3:9927413-9927432 None:intergenic 20.0%
!! TTCTTTGAATAATTCTATCT+GGG + chr1_3:9928174-9928193 None:intergenic 20.0%
!! ATTCTTTGAATAATTCTATC+TGG + chr1_3:9928175-9928194 None:intergenic 20.0%
!! TAGCACAAAATAAATACTAT+TGG + chr1_3:9928252-9928271 None:intergenic 20.0%
!! TTAATAAAGTGTAGAAAAAG+TGG + chr1_3:9929261-9929280 None:intergenic 20.0%
!! TGGATAATTAATTCAATTCA+CGG - chr1_3:9929313-9929332 Msa0103000:five_prime_UTR 20.0%
! ATCTGAGAAGATTAAAATCA+AGG - chr1_3:9926906-9926925 Msa0103000:three_prime_UTR 25.0%
!!! GTAACAAATTTTTTCCTTAC+GGG + chr1_3:9926956-9926975 None:intergenic 25.0%
!!! TATTTTTAACAAGTGCTGTA+AGG - chr1_3:9927133-9927152 Msa0103000:three_prime_UTR 25.0%
!!! ATTTTTAACAAGTGCTGTAA+GGG - chr1_3:9927134-9927153 Msa0103000:three_prime_UTR 25.0%
!!! ATATGTTGACTTTGTCAAAT+CGG + chr1_3:9927196-9927215 None:intergenic 25.0%
!! CTAAAAGTATAGTCAAAAAG+AGG + chr1_3:9927466-9927485 None:intergenic 25.0%
!!! TTTTGTTATGTGTAACAGAA+TGG + chr1_3:9927710-9927729 None:intergenic 25.0%
! TTACTATCAGTTGAAGAATT+AGG - chr1_3:9927794-9927813 Msa0103000:intron 25.0%
!! CTCTTTTACTCATTTCAATT+AGG + chr1_3:9928044-9928063 None:intergenic 25.0%
! CTAGAAAGAAAGAAACAAAA+AGG - chr1_3:9928064-9928083 Msa0103000:three_prime_UTR 25.0%
! TGTTTCCTTATATAATAAGC+AGG - chr1_3:9928130-9928149 Msa0103000:three_prime_UTR 25.0%
!!! AGTATTTATTTTGTGCTAGT+AGG - chr1_3:9928254-9928273 Msa0103000:exon 25.0%
! TAAAAAATTTCAGCAAGCTT+TGG - chr1_3:9928451-9928470 Msa0103000:intron 25.0%
!! TCATGACGGAAAATAATTAA+CGG + chr1_3:9928478-9928497 None:intergenic 25.0%
! ACATAAATCAAAAGTCATGA+CGG + chr1_3:9928492-9928511 None:intergenic 25.0%
! TGCGAAGTATAAAATCTTAA+CGG - chr1_3:9928864-9928883 Msa0103000:intron 25.0%
! TCCTTCTATATACAAATGAA+AGG - chr1_3:9928947-9928966 Msa0103000:intron 25.0%
! TCCTTTCATTTGTATATAGA+AGG + chr1_3:9928951-9928970 None:intergenic 25.0%
! TTACAACGATATCAAGTATA+GGG + chr1_3:9929190-9929209 None:intergenic 25.0%
! TTTACAACGATATCAAGTAT+AGG + chr1_3:9929191-9929210 None:intergenic 25.0%
! GTTGTAAACTACCAAAAATA+TGG - chr1_3:9929203-9929222 Msa0103000:intron 25.0%
!! GATACTTGTTATGATGTCAA+GGG + chr1_3:9927104-9927123 None:intergenic 30.0%
!! TGATACTTGTTATGATGTCA+AGG + chr1_3:9927105-9927124 None:intergenic 30.0%
ATCATAATCATTGCACTACT+AGG - chr1_3:9927220-9927239 Msa0103000:three_prime_UTR 30.0%
AGGAAAATCGAATTACTAGT+TGG - chr1_3:9927240-9927259 Msa0103000:intron 30.0%
TAGTTATTAGCTGCAATGTT+TGG - chr1_3:9927314-9927333 Msa0103000:intron 30.0%
CTGCAATGTTTAGCATAAAT+AGG - chr1_3:9927359-9927378 Msa0103000:intron 30.0%
TTTGGTTAATACTGGAAACA+AGG + chr1_3:9927395-9927414 None:intergenic 30.0%
TGTATTTGGAGATTCAATCA+TGG - chr1_3:9927550-9927569 Msa0103000:intron 30.0%
ATACTGGAAATAACAACAAC+AGG - chr1_3:9927573-9927592 Msa0103000:intron 30.0%
AGAATATCTTCCAGCATATT+TGG - chr1_3:9927823-9927842 Msa0103000:intron 30.0%
TCCTTTGACATCAAAAATAG+AGG - chr1_3:9927913-9927932 Msa0103000:three_prime_UTR 30.0%
!!! ACCTCTATTTTTGATGTCAA+AGG + chr1_3:9927917-9927936 None:intergenic 30.0%
!! TTAGAGATTGATTTGATCGA+TGG + chr1_3:9927960-9927979 None:intergenic 30.0%
GAATATATTGTGAAGCTGAA+AGG - chr1_3:9928191-9928210 Msa0103000:three_prime_UTR 30.0%
TATAAGCTGAGTCTACTAAA+AGG + chr1_3:9928355-9928374 None:intergenic 30.0%
CTCAGCTTATAATTTCTACA+AGG - chr1_3:9928364-9928383 Msa0103000:CDS 30.0%
!!! GGTAGTTTTATTATTCCACA+CGG - chr1_3:9928385-9928404 Msa0103000:CDS 30.0%
!!! GTAGTTTTATTATTCCACAC+GGG - chr1_3:9928386-9928405 Msa0103000:CDS 30.0%
!!! AGCTTGCTGAAATTTTTTAG+AGG + chr1_3:9928450-9928469 None:intergenic 30.0%
! CATGACGGAAAATAATTAAC+GGG + chr1_3:9928477-9928496 None:intergenic 30.0%
TGAAAGGAAATGTATCAACT+AGG - chr1_3:9928963-9928982 Msa0103000:intron 30.0%
TATTCAATGTACCACCAATT+GGG - chr1_3:9929003-9929022 Msa0103000:intron 30.0%
ACTTGGAAAATTCTGCTTAA+AGG + chr1_3:9929138-9929157 None:intergenic 30.0%
ATATAAACAATCCTGCTACT+TGG + chr1_3:9929155-9929174 None:intergenic 30.0%
AAGTAGCAGGATTGTTTATA+TGG - chr1_3:9929154-9929173 Msa0103000:intron 30.0%
ATATGGTTAATTCATCTCAG+TGG - chr1_3:9929293-9929312 Msa0103000:five_prime_UTR 30.0%
AAATCAAGGCAAAAACCCAA+AGG - chr1_3:9926920-9926939 Msa0103000:three_prime_UTR 35.0%
AAGAGAGAGGGAAAACAAAA+GGG + chr1_3:9926989-9927008 None:intergenic 35.0%
TTAAATCCAAGCAAGAGAGA+GGG + chr1_3:9927001-9927020 None:intergenic 35.0%
GACTTTGTCAAATCGGTATA+AGG + chr1_3:9927189-9927208 None:intergenic 35.0%
TTTCCAGCAGTGTTTGTATT+TGG - chr1_3:9927536-9927555 Msa0103000:intron 35.0%
GAAAATCTTTGCCATATGGA+GGG + chr1_3:9927622-9927641 None:intergenic 35.0%
TGAAAATCTTTGCCATATGG+AGG + chr1_3:9927623-9927642 None:intergenic 35.0%
CCTTGAAAATCTTTGCCATA+TGG + chr1_3:9927626-9927645 None:intergenic 35.0%
! CCATATGGCAAAGATTTTCA+AGG - chr1_3:9927623-9927642 Msa0103000:intron 35.0%
!! TATGGCAAAGATTTTCAAGG+AGG - chr1_3:9927626-9927645 Msa0103000:intron 35.0%
TGGAAGATTTAGCAATGGAA+AGG - chr1_3:9927658-9927677 Msa0103000:intron 35.0%
ATAGTACCTATAAGATCTGC+TGG + chr1_3:9927686-9927705 None:intergenic 35.0%
AGATTTGGATCCAAATATGC+TGG + chr1_3:9927836-9927855 None:intergenic 35.0%
AATTCACTAGGCTGAAGATT+TGG + chr1_3:9927851-9927870 None:intergenic 35.0%
AAATCTTCAGCCTAGTGAAT+TGG - chr1_3:9927850-9927869 Msa0103000:three_prime_UTR 35.0%
AGCTGCCTGCTTATTATATA+AGG + chr1_3:9928138-9928157 None:intergenic 35.0%
ACTAAAAGGTCAGAGTATGA+AGG + chr1_3:9928341-9928360 None:intergenic 35.0%
! ATGAGTGCACTTAAAAGCAT+GGG - chr1_3:9928783-9928802 Msa0103000:intron 35.0%
AAAGCATGGGAGAAAACATT+TGG - chr1_3:9928796-9928815 Msa0103000:intron 35.0%
!! AACTTGCTGAAAACTCATAG+AGG - chr1_3:9928825-9928844 Msa0103000:intron 35.0%
! ACTTGCTGAAAACTCATAGA+GGG - chr1_3:9928826-9928845 Msa0103000:intron 35.0%
AAATGTATCAACTAGGTGCA+AGG - chr1_3:9928970-9928989 Msa0103000:intron 35.0%
GTATTCAATGTACCACCAAT+TGG - chr1_3:9929002-9929021 Msa0103000:intron 35.0%
ACGGGTCTTAAGAATCCTTT+GGG + chr1_3:9926938-9926957 None:intergenic 40.0%
TACGGGTCTTAAGAATCCTT+TGG + chr1_3:9926939-9926958 None:intergenic 40.0%
AAGGATTCTTAAGACCCGTA+AGG - chr1_3:9926939-9926958 Msa0103000:three_prime_UTR 40.0%
CAAGAGAGAGGGAAAACAAA+AGG + chr1_3:9926990-9927009 None:intergenic 40.0%
CTTAAATCCAAGCAAGAGAG+AGG + chr1_3:9927002-9927021 None:intergenic 40.0%
!! ATGCAATGTTCTAAGAACCG+AGG - chr1_3:9927065-9927084 Msa0103000:three_prime_UTR 40.0%
! GGAGAAGTGTAGTGCAAATT+GGG + chr1_3:9927445-9927464 None:intergenic 40.0%
! AGGAGAAGTGTAGTGCAAAT+TGG + chr1_3:9927446-9927465 None:intergenic 40.0%
TCTCCAAATACAAACACTGC+TGG + chr1_3:9927542-9927561 None:intergenic 40.0%
GGAGATTCAATCATGGATAC+TGG - chr1_3:9927557-9927576 Msa0103000:intron 40.0%
!! AATTTGCACTGAGTTGGTGT+TGG + chr1_3:9927599-9927618 None:intergenic 40.0%
TTTCAAGGAGGAATTCCAAC+TGG - chr1_3:9927638-9927657 Msa0103000:intron 40.0%
CCATTGCTAAATCTTCCAGT+TGG + chr1_3:9927656-9927675 None:intergenic 40.0%
CCAACTGGAAGATTTAGCAA+TGG - chr1_3:9927653-9927672 Msa0103000:intron 40.0%
AAGGTTCCAGCAGATCTTAT+AGG - chr1_3:9927677-9927696 Msa0103000:intron 40.0%
CATGAGTGCACTTAAAAGCA+TGG - chr1_3:9928782-9928801 Msa0103000:intron 40.0%
! AGCAGAATTTTCCAAGTAGC+AGG - chr1_3:9929141-9929160 Msa0103000:intron 40.0%
! TGTTTTCCCTCTCTCTTGCT+TGG - chr1_3:9926992-9927011 Msa0103000:three_prime_UTR 45.0%
! TTAACAAGTGCTGTAAGGGC+AGG - chr1_3:9927138-9927157 Msa0103000:three_prime_UTR 45.0%
! TAACAAGTGCTGTAAGGGCA+GGG - chr1_3:9927139-9927158 Msa0103000:three_prime_UTR 45.0%
TGTCAAATCGGTATAAGGCG+TGG + chr1_3:9927184-9927203 None:intergenic 45.0%
! ACTGCTGGAAACGAAGCATT+TGG + chr1_3:9927527-9927546 None:intergenic 45.0%
CAGTGCAAATTCCCTCCATA+TGG - chr1_3:9927608-9927627 Msa0103000:intron 45.0%
CAGCCTAGTGAATTGGTTAC+CGG - chr1_3:9927857-9927876 Msa0103000:three_prime_UTR 45.0%
ACACCGGTAACCAATTCACT+AGG + chr1_3:9927863-9927882 None:intergenic 45.0%
! ACCGGTGTTAACTTTGCTTC+CGG - chr1_3:9927875-9927894 Msa0103000:three_prime_UTR 45.0%
ACCGGAAGCAAAGTTAACAC+CGG + chr1_3:9927879-9927898 None:intergenic 45.0%
! GTGAAGCTGAAAGGACTAGT+TGG - chr1_3:9928200-9928219 Msa0103000:three_prime_UTR 45.0%
TTACAAGGATCAAACCCGTG+TGG + chr1_3:9928403-9928422 None:intergenic 45.0%
TGTATCAACTAGGTGCAAGG+AGG - chr1_3:9928973-9928992 Msa0103000:intron 45.0%
!!! ATTAATCATTTTAAAAAATA+TGG - chr1_3:9929276-9929295 Msa0103000:five_prime_UTR 5.0%
AGGGTCTCATGAGCTTACCT+CGG + chr1_3:9927085-9927104 None:intergenic 50.0%
! GCTGGAAACGAAGCATTTGG+TGG + chr1_3:9927524-9927543 None:intergenic 50.0%
GGAGGGAATTTGCACTGAGT+TGG + chr1_3:9927605-9927624 None:intergenic 50.0%
GGTGTTAACTTTGCTTCCGG+TGG - chr1_3:9927878-9927897 Msa0103000:three_prime_UTR 50.0%
AGGATCATATCCTGCACCAC+CGG + chr1_3:9927897-9927916 None:intergenic 50.0%
CAACTAGGTGCAAGGAGGAT+AGG - chr1_3:9928978-9928997 Msa0103000:intron 50.0%
TGAAACGGTACGCACCCAAT+TGG + chr1_3:9929020-9929039 None:intergenic 50.0%
CGTACCGTTTCAGAGAACAG+TGG - chr1_3:9929028-9929047 Msa0103000:intron 50.0%
! CCAGCCACTGTTCTCTGAAA+CGG + chr1_3:9929035-9929054 None:intergenic 50.0%
TTTCAGAGAACAGTGGCTGG+AGG - chr1_3:9929035-9929054 Msa0103000:intron 50.0%
AACTTTGCTTCCGGTGGTGC+AGG - chr1_3:9927884-9927903 Msa0103000:three_prime_UTR 55.0%
AACGGTACGCACCCAATTGG+TGG + chr1_3:9929017-9929036 None:intergenic 55.0%
CCGTTTCAGAGAACAGTGGC+TGG - chr1_3:9929032-9929051 Msa0103000:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 9926876 9929344 9926876 ID=Msa0103000;Name=Msa0103000
chr1_3 mRNA 9926876 9929344 9926876 ID=Msa0103000-mRNA-1;Parent=Msa0103000;Name=Msa0103000-mRNA-1;_AED=0.35;_eAED=0.35;_QI=106|1|1|1|0.33|0|4|806|93
chr1_3 exon 9926876 9927251 9926876 ID=Msa0103000-mRNA-1:exon:4090;Parent=Msa0103000-mRNA-1
chr1_3 exon 9927835 9928416 9927835 ID=Msa0103000-mRNA-1:exon:4089;Parent=Msa0103000-mRNA-1
chr1_3 exon 9928522 9928677 9928522 ID=Msa0103000-mRNA-1:exon:4088;Parent=Msa0103000-mRNA-1
chr1_3 exon 9929265 9929344 9929265 ID=Msa0103000-mRNA-1:exon:4087;Parent=Msa0103000-mRNA-1
chr1_3 five_prime_UTR 9929265 9929344 9929265 ID=Msa0103000-mRNA-1:five_prime_utr;Parent=Msa0103000-mRNA-1
chr1_3 five_prime_UTR 9928652 9928677 9928652 ID=Msa0103000-mRNA-1:five_prime_utr;Parent=Msa0103000-mRNA-1
chr1_3 CDS 9928522 9928651 9928522 ID=Msa0103000-mRNA-1:cds;Parent=Msa0103000-mRNA-1
chr1_3 CDS 9928265 9928416 9928265 ID=Msa0103000-mRNA-1:cds;Parent=Msa0103000-mRNA-1
chr1_3 three_prime_UTR 9927835 9928264 9927835 ID=Msa0103000-mRNA-1:three_prime_utr;Parent=Msa0103000-mRNA-1
chr1_3 three_prime_UTR 9926876 9927251 9926876 ID=Msa0103000-mRNA-1:three_prime_utr;Parent=Msa0103000-mRNA-1
Gene Sequence

>Msa0103000

ATGGATACTGGAAATAACAACAACAGGCCAACACCAACTCAGTGCAAATTCCCTCCATATGGCAAAGATTTTCAAGGAGGAATTCCAACTGGAAGATTTAGCAATGGAAAGGTTCCAGCAGATCTTATAGTTGAAGAATTAGGCATTAAAGAATATCTTCCAGCATATTTGGATCCAAATCTTCAGCCTAGTGAATTGGTTACCGGTGTTAACTTTGCTTCCGGTGGTGCAGGATATGATCCTTTGACATCAAAAATAGAGGTATCTATTTTTTCAGAATAA
Protein sequence

>Msa0103000

MDTGNNNNRPTPTQCKFPPYGKDFQGGIPTGRFSNGKVPADLIVEELGIKEYLPAYLDPNLQPSELVTGVNFASGGAGYDPLTSKIEVSIFSE*