AlfalfaGEDB | Msa0999270

M. sativa cultivar zhongmu-4 / Msa0999270

KEGG: K19476
GO: -
PFAM: PF03398.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999270	RHN52891.1	94.787	633	32	1	1	632	57	689	0.0	1219

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999270	A0A396HKA5	94.787	633	32	1	1	632	57	689	0.0	1219

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0279210	Msa0999270	0.824888	6.324454e-54	-8.615850e-47
Msa0279250	Msa0999270	0.827131	1.853449e-54	-8.615850e-47
Msa0318970	Msa0999270	0.824869	6.389695e-54	-8.615850e-47
Msa0545550	Msa0999270	0.803904	2.790361e-49	-8.615850e-47
Msa0899500	Msa0999270	0.919414	4.640734e-87	-8.615850e-47
Msa0999270	Msa1131670	0.806545	7.809647e-50	-8.615850e-47
Msa0999270	Msa1207460	0.800284	1.549020e-48	-8.615850e-47
Msa0999270	Msa1262990	0.826532	2.576216e-54	-8.615850e-47
Msa0999270	Msa1308330	0.804273	2.338283e-49	-8.615850e-47
Msa0999270	Msa1317480	0.805120	1.555807e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999270	MtrunA17_Chr6g0485461	94.787	633	32	1	1	632	57	689	0.0	1219
Msa0999270	MtrunA17_Chr8g0390551	56.508	653	249	11	1	631	1	640	0.0	652
Msa0999270	MtrunA17_Chr2g0331801	47.222	180	95	0	1	180	1	180	2.09e-51	183
Msa0999270	MtrunA17_Chr4g0004491	52.299	174	82	1	1	174	4	176	2.50e-51	181
Msa0999270	MtrunA17_Chr8g0370931	33.333	189	116	2	4	182	20	208	1.79e-29	121
Msa0999270	MtrunA17_Chr6g0487991	32.418	182	113	2	5	176	1	182	1.02e-25	113
Msa0999270	MtrunA17_Chr6g0464791	30.208	192	124	2	1	182	3	194	5.37e-24	106
Msa0999270	MtrunA17_Chr1g0209771	29.208	202	126	3	1	192	3	197	1.07e-23	102
Msa0999270	MtrunA17_Chr2g0324121	33.526	173	106	2	1	164	1	173	2.09e-23	104
Msa0999270	MtrunA17_Chr7g0214961	28.000	175	117	2	4	169	2	176	2.15e-18	88.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999270	AT4G32350.1	59.459	185	75	0	1	185	1	185	3.04e-71	244
Msa0999270	AT4G32350.1	33.333	306	160	9	330	631	466	731	7.49e-30	125
Msa0999270	AT4G32350.3	59.459	185	75	0	1	185	1	185	3.04e-71	244
Msa0999270	AT4G32350.3	33.333	306	160	9	330	631	466	731	7.49e-30	125
Msa0999270	AT1G79910.1	54.444	180	82	0	1	180	1	180	4.79e-62	211
Msa0999270	AT1G52315.1	42.458	179	98	2	10	185	10	186	3.12e-37	142
Msa0999270	AT1G79910.3	52.459	122	58	0	59	180	4	125	1.03e-35	137
Msa0999270	AT1G79910.2	52.066	121	58	0	60	180	46	166	1.39e-34	135
Msa0999270	AT4G35730.1	34.921	189	113	2	4	182	20	208	1.06e-30	126
Msa0999270	AT4G32350.2	53.608	97	45	0	89	185	1	97	3.93e-30	126

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Find 155 sgRNAs with CRISPR-Local

Find 311 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCCTGACCATTCTAA+CGG	0.623323	6_4:-81067514	Msa0999270:CDS
AAACTGATCGAGGTGCACGA+GGG	0.725458	6_4:+81066429	None:intergenic
AAAGGTGTCTATTGGAAGGA+GGG	0.594393	6_4:-81067310	Msa0999270:CDS
AAATCTAAGCTCCCTGAAAA+AGG	0.293667	6_4:-81067424	Msa0999270:CDS
AAGCATAGTTGAAGATCGAT+CGG	0.624435	6_4:+81070710	None:intergenic
AAGGATTCAAGCCAATCACT+AGG	0.610407	6_4:-81067405	Msa0999270:CDS
AAGTCGTCATGCACATCAAA+TGG	0.425691	6_4:-81066483	Msa0999270:CDS
AATATAGTAGAAAATTATAA+AGG	0.426004	6_4:+81065935	None:intergenic
AATCTACCAAGGGATAGCCA+TGG	0.679655	6_4:-81067370	Msa0999270:CDS
ACAAGATGTCAATGTTAACT+CGG	0.603860	6_4:+81069273	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CGAAGAAGAAGAAAGAAAAA+AGG	+	chr6_4:81065920-81065939	None:intergenic	30.0%
AAAAAAATCGAACCATTTCG+CGG	+	chr6_4:81066112-81066131	None:intergenic	30.0%
CGATTTACTTCAAAATTCGA+AGG	+	chr6_4:81066410-81066429	None:intergenic	30.0%
AAAATCAGGGACTAATTTAG+TGG	+	chr6_4:81066981-81067000	None:intergenic	30.0%
AGTAAAATTTGACAGAGTCA+GGG	-	chr6_4:81067205-81067224	Msa0999270:CDS	30.0%
TAAAATTCTCACAAGTCCTA+AGG	+	chr6_4:81067383-81067402	None:intergenic	30.0%
ACAAGATGTCAATGTTAACT+CGG	+	chr6_4:81067501-81067520	None:intergenic	30.0%
TAAATTCACATAGGAAGACA+AGG	+	chr6_4:81067819-81067838	None:intergenic	30.0%
ATGGAACACTAAATTCACAT+AGG	+	chr6_4:81067828-81067847	None:intergenic	30.0%
AATTGGAATTTCAACTCTCA+AGG	-	chr6_4:81068036-81068055	Msa0999270:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	81065891	81070902	81065891	ID=Msa0999270;Name=Msa0999270
chr6_4	mRNA	81065891	81070902	81065891	ID=Msa0999270-mRNA-1;Parent=Msa0999270;Name=Msa0999270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187\|1\|1\|1\|1\|1\|6\|377\|632
chr6_4	exon	81065891	81067608	81065891	ID=Msa0999270-mRNA-1:exon:15465;Parent=Msa0999270-mRNA-1
chr6_4	exon	81067818	81067952	81067818	ID=Msa0999270-mRNA-1:exon:15464;Parent=Msa0999270-mRNA-1
chr6_4	exon	81068045	81068189	81068045	ID=Msa0999270-mRNA-1:exon:15463;Parent=Msa0999270-mRNA-1
chr6_4	exon	81069208	81069311	81069208	ID=Msa0999270-mRNA-1:exon:15462;Parent=Msa0999270-mRNA-1
chr6_4	exon	81070112	81070244	81070112	ID=Msa0999270-mRNA-1:exon:15461;Parent=Msa0999270-mRNA-1
chr6_4	exon	81070675	81070902	81070675	ID=Msa0999270-mRNA-1:exon:15460;Parent=Msa0999270-mRNA-1
chr6_4	five_prime_UTR	81070716	81070902	81070716	ID=Msa0999270-mRNA-1:five_prime_utr;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81070675	81070715	81070675	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81070112	81070244	81070112	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81069208	81069311	81069208	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81068045	81068189	81068045	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81067818	81067952	81067818	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	CDS	81066268	81067608	81066268	ID=Msa0999270-mRNA-1:cds;Parent=Msa0999270-mRNA-1
chr6_4	three_prime_UTR	81065891	81066267	81065891	ID=Msa0999270-mRNA-1:three_prime_utr;Parent=Msa0999270-mRNA-1

Gene Sequence

>Msa0999270

ATGCTTGACGGCTTACTCGGTCGTGGATTCGCCGCGAAATGTAAGTCGTTGATTAAGTTGACGAAGAATCGGATCGATGTGATAAGGAGGAAGAGGAAAGCGACAGAGAAGTTTTTGAAGAACGATATTGGTGATCTGTTGGTGAATGGATTGGATGTTAATGCTTATGGAAGGGCTGAAGGACTCATTGCCGAGTTAACATTGACATCTTGTTATGATTTTGTTGAGTGGACCTGTGATTTTGTGTTGAAGCACCTCTCAGTGCTACAGAAACTGAGTGGATGCCCTGAGGAATGCAGGGAAGCTATAGCGTGTCTGATTATTGCTGCAGCAAGATTTTCTGATCTACCAGAGTTACGCGACCTTCGGCAAATATTTCAGGAGAGATACGGAAACTCTCTAGAATGTTATGTCAATCAAGAGTTTGCTGCAAATTTGAATCCGAAGTCTTTTACCTTGGAGCAGAAGGTTCGGTTGATGCAAGAGATTTCTTCGGAGTTTTCAATAAAGTGGGACTCCAAAGATTTTGAACTGAGGATGTCAAAATCCTCTGCTAAGGGCCACAAAACTCATAAATCTGACCATTCAGTTGGGAAACCATTACAAGGCAAGTCTGAGATTTTGTTAGATAAAAGTCCTGACCATTCTAACGGTGGGCACAAATATCGGAATGGTAAGGAAGCTGCTGTCTCAAAAGCAGATGAAAATTATCTTCATCCCAAATCTAAGCTCCCTGAAAAAGGATTCAAGCCAATCACTAGGTATGATGAAGTCAATCTACCAAGGGATAGCCATGGCAATCCGTTGCTAGGAAAAGAAGAGCTTACTTCTCAGAAAGGTGTCTATTGGAAGGAGGGTAGTATGCTAAAACCACCAATTGGATGTTCTTCTCAGGATAAGCGAGTGCACCAATTTGACGATGGTGATTTACATGATAGAAGGGGGAATGCCACCCGTGTGAGAGAAACCCCGGACACTGCCACTGCTAGGAAGTCTCCGAGTCATGCAGGATTTCAATCAAAGAACAATTTGAATGAACCATTTGCTGTTAATCATGGTGGACTACCTGATCTTGATAACTCTCAGAGAAAAGTTCAAAAGGATGAAACTCCTAAGGTAAAGCCGTACTACAGTAATGCCATCCCACCACCTTATGTAAAACCTAATTCTAAACTCAAGACGAGCACACAAAGAACTGAATTAGCATCTTCACATATTGACAATGATGGCATCCCAACAAATCCTTCAATACATCAAAAGCCTGACGCTGCTTCTACTACAGAGAAAATCCGATCAGGTTTGGATAATTCTGAACATGATTTGCAGGCCACTAGACATGCTCGACTGAGTAAACATGATTATGAGAAAGAATCCCCTGTTCTTGGAGAGGCCAGAGAATTTCCTATATTAAAACAAAAATCTTCGAGGCGCAGGCACTCGAAATCACGGTCTACACACAATGATGCCAATAAAGAAGATGCTGAAGCTGCGAGAAAATCAAGGAGCAAGAGACGAGACGAATCAAAACGTGGCCTACAAATTTTGTTTGACGATGAGAAACATAAAAAAGACGAAGAAGAAAGGGTTATAGATAGATTGCTGATCCATTATAGCAAAAAGCCAACCATCAATGTGCCTGAGAAAGCAAGAAGAAGGTCTAAAAGTCGTCATGCACATCAAATGGATGATGGACCTGATAAGACACCAGATATGGTCCCTCGTGCACCTCGATCAGTTTCTCTTCCTCACGAACAATCACAAGAGATGGAAGTCAACAAAGTGTTTACTCGAGCTGCTTCGTTTCAGCCAGATAGATCAAAAGAAGCCCGGCATGTGCATCCCAACTTACCCGATTATGATGATTTAGCTGCTAGGCTTGCGGCCTTGAGAGGAACATAA

Protein sequence

>Msa0999270

MLDGLLGRGFAAKCKSLIKLTKNRIDVIRRKRKATEKFLKNDIGDLLVNGLDVNAYGRAEGLIAELTLTSCYDFVEWTCDFVLKHLSVLQKLSGCPEECREAIACLIIAAARFSDLPELRDLRQIFQERYGNSLECYVNQEFAANLNPKSFTLEQKVRLMQEISSEFSIKWDSKDFELRMSKSSAKGHKTHKSDHSVGKPLQGKSEILLDKSPDHSNGGHKYRNGKEAAVSKADENYLHPKSKLPEKGFKPITRYDEVNLPRDSHGNPLLGKEELTSQKGVYWKEGSMLKPPIGCSSQDKRVHQFDDGDLHDRRGNATRVRETPDTATARKSPSHAGFQSKNNLNEPFAVNHGGLPDLDNSQRKVQKDETPKVKPYYSNAIPPPYVKPNSKLKTSTQRTELASSHIDNDGIPTNPSIHQKPDAASTTEKIRSGLDNSEHDLQATRHARLSKHDYEKESPVLGEAREFPILKQKSSRRRHSKSRSTHNDANKEDAEAARKSRSKRRDESKRGLQILFDDEKHKKDEEERVIDRLLIHYSKKPTINVPEKARRRSKSRHAHQMDDGPDKTPDMVPRAPRSVSLPHEQSQEMEVNKVFTRAASFQPDRSKEARHVHPNLPDYDDLAARLAALRGT*