AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1038300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1038300 MtrunA17_Chr7g0274181 93.689 206 9 1 1 206 1 202 7.06e-136 379
Msa1038300 MtrunA17_Chr1g0190671 71.901 121 34 0 68 188 24 144 4.54e-64 194
Msa1038300 MtrunA17_Chr1g0190661 71.074 121 35 0 68 188 24 144 2.01e-63 193
Msa1038300 MtrunA17_Chr1g0190641 70.000 120 36 0 71 190 22 141 3.28e-63 192
Msa1038300 MtrunA17_Chr1g0190651 70.732 123 36 0 68 190 24 146 9.26e-63 191
Msa1038300 MtrunA17_Chr6g0484021 50.000 154 60 2 61 197 35 188 4.63e-43 152
Msa1038300 MtrunA17_Chr6g0483971 47.692 130 64 2 61 188 15 142 9.82e-37 130
Msa1038300 MtrunA17_Chr6g0484031 47.445 137 61 2 61 190 15 147 1.86e-33 125
Msa1038300 MtrunA17_Chr6g0484041 44.853 136 58 3 68 188 34 167 5.72e-32 121
Msa1038300 MtrunA17_Chr3g0145981 51.515 66 32 0 1 66 24 89 6.38e-17 77.8
Msa1038300 MtrunA17_Chr8g0370831 68.293 41 13 0 22 62 22 62 5.80e-13 66.2
Msa1038300 MtrunA17_Chr8g0370851 50.000 56 28 0 6 61 7 62 6.71e-12 63.2
Msa1038300 MtrunA17_Chr8g0370821 67.500 40 13 0 22 61 33 72 1.12e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1038300 AT3G04720.1 71.292 209 52 3 3 203 2 210 3.70e-97 281
Msa1038300 AT3G12500.1 68.293 41 12 1 22 61 33 73 8.62e-12 63.5

Find 87 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTAGACATGGGAAAATT+TGG 0.218092 7_1:-75117873 Msa1038300:exon
ATGATGCAGGTGACAAATTC+AGG 0.293677 7_1:-75117239 Msa1038300:intron
CAGCAATGGAGGGTTGGATT+TGG 0.326401 7_1:-75117177 Msa1038300:CDS
TACTCATGGCGTAGCAAGTA+TGG 0.353266 7_1:-75117549 Msa1038300:CDS
CTTCTTGTGGAAAGTGTCTC+AGG 0.353594 7_1:-75117485 Msa1038300:CDS
TGGCTGCAACAAAGGTTGTT+TGG 0.365069 7_1:+75117762 None:intergenic
ATTCTAGTCAACAATATAAC+AGG 0.389534 7_1:+75116796 None:intergenic
GCTTGACCACGAGGTCCAAC+AGG 0.397549 7_1:+75117507 None:intergenic
GGAGGGTTGGATTTGGATGT+AGG 0.409363 7_1:-75117170 Msa1038300:CDS
ATCACGGTGCGGCCTCACTC+TGG 0.419143 7_1:-75116729 Msa1038300:three_prime_UTR
AAGTCCCATCCATGTTGCTC+TGG 0.422201 7_1:+75117618 None:intergenic
TATTGTAAGCTATCAGTTTG+TGG 0.428774 7_1:-75117096 Msa1038300:CDS
TCTTATTAAATTATTGCCAA+TGG 0.460327 7_1:-75117966 Msa1038300:five_prime_UTR
GCTATCAGTTTGTGGATTGT+GGG 0.462963 7_1:-75117088 Msa1038300:CDS
AGATGGCAGAGGATACCAAC+AGG 0.473028 7_1:-75117126 Msa1038300:CDS
AACCTTTGTTGCAGCCAATA+CGG 0.476377 7_1:-75117756 Msa1038300:CDS
TACTTGCTACGCCATGAGTA+AGG 0.479661 7_1:+75117552 None:intergenic
CTGAGGCAGAGGAAGCAGAT+TGG 0.484338 7_1:+75117008 None:intergenic
AACCGTATTGGCTGCAACAA+AGG 0.489204 7_1:+75117754 None:intergenic
AAATTCAGGGACGGGAGCAC+AGG 0.490592 7_1:-75117225 Msa1038300:CDS
AGCTATCAGTTTGTGGATTG+TGG 0.492406 7_1:-75117089 Msa1038300:CDS
AGTGGTTGTGTTTGTAACAA+AGG 0.503596 7_1:-75116968 Msa1038300:three_prime_UTR
GAACAATGCGGTAAACAAGC+TGG 0.511319 7_1:-75117798 Msa1038300:CDS
TTATTGTTCAGCATCAATGA+TGG 0.515481 7_1:+75117034 None:intergenic
TGGTACCACACCAACCGTAT+TGG 0.526780 7_1:+75117742 None:intergenic
TAAGTGCTTATTGTTCAACT+TGG 0.529463 7_1:-75117587 Msa1038300:CDS
TGACAATTACTCTGACAGTT+CGG 0.538086 7_1:+75117702 None:intergenic
CAGGTGACAAATTCAGGGAC+GGG 0.545443 7_1:-75117233 Msa1038300:CDS
GAATATTCCAACCATAAAAG+TGG 0.545641 7_1:-75116777 Msa1038300:three_prime_UTR
GTGCTGTAGTAATTAATAAG+TGG 0.545844 7_1:-75116986 Msa1038300:three_prime_UTR
TTACTACAGCACTGAGGCAG+AGG 0.549229 7_1:+75116997 None:intergenic
CCTCGTGGTCAAGCTTCTTG+TGG 0.556286 7_1:-75117498 Msa1038300:CDS
TCAATGCAGCAATGGAGGGT+TGG 0.558488 7_1:-75117183 Msa1038300:CDS
CTGATGTGATGGAGCTCCGG+TGG 0.560689 7_1:-75116753 Msa1038300:three_prime_UTR
AAGTGCTTATTGTTCAACTT+GGG 0.564174 7_1:-75117586 Msa1038300:CDS
AATGATGGAGAACAGAGGAT+TGG 0.564526 7_1:+75117049 None:intergenic
ATGGAGCTCCGGTGGGATCA+CGG 0.572433 7_1:-75116745 Msa1038300:three_prime_UTR
TTATTGCCAATGGAAAATGA+TGG 0.576576 7_1:-75117956 Msa1038300:five_prime_UTR
GTAGATCAATGCAGCAATGG+AGG 0.578522 7_1:-75117188 Msa1038300:CDS
TTTGTTGCAGCCAATACGGT+TGG 0.578744 7_1:-75117752 Msa1038300:CDS
ATAAAAGTGGTCTGATGTGA+TGG 0.579232 7_1:-75116764 Msa1038300:three_prime_UTR
TGATGCAGGTGACAAATTCA+GGG 0.583955 7_1:-75117238 Msa1038300:intron
GACATATACCCATGTGCATG+CGG 0.585883 7_1:+75118029 None:intergenic
GGTCTGATGTGATGGAGCTC+CGG 0.586642 7_1:-75116756 Msa1038300:three_prime_UTR
ATGCAAGCAAACCTTACTCA+TGG 0.586811 7_1:-75117563 Msa1038300:CDS
ATCAACCAGAGCAACATGGA+TGG 0.587569 7_1:-75117623 Msa1038300:CDS
TCAACCAGAGCAACATGGAT+GGG 0.598724 7_1:-75117622 Msa1038300:CDS
AAATTGTATTAGATTAGACA+TGG 0.600324 7_1:-75117883 Msa1038300:exon
CATGGCGTAGCAAGTATGGT+TGG 0.600676 7_1:-75117545 Msa1038300:CDS
TAGATCAATGCAGCAATGGA+GGG 0.603290 7_1:-75117187 Msa1038300:CDS
AGGCCGCACCGTGATCCCAC+CGG 0.604066 7_1:+75116737 None:intergenic
TTACAATAAGATGTCCCTGT+TGG 0.604159 7_1:+75117111 None:intergenic
GCAGGTGACAAATTCAGGGA+CGG 0.605105 7_1:-75117234 Msa1038300:intron
TTTATGTCACGATATTGACT+AGG 0.609065 7_1:+75117993 None:intergenic
AGTAATTGTCATGGCAGTAG+TGG 0.614410 7_1:-75117690 Msa1038300:CDS
GTCACGATATTGACTAGGCA+TGG 0.614770 7_1:+75117998 None:intergenic
TGTGGCCCTGTTGGACCTCG+TGG 0.621769 7_1:-75117513 Msa1038300:CDS
TGTCATGGCAGTAGTGGTGG+TGG 0.621989 7_1:-75117684 Msa1038300:CDS
TTCTTGTGGAAAGTGTCTCA+GGG 0.622227 7_1:-75117484 Msa1038300:intron
AATTGTCATGGCAGTAGTGG+TGG 0.624496 7_1:-75117687 Msa1038300:CDS
AAGCCTAGCGGTATTAACAT+TGG 0.627981 7_1:-75117847 Msa1038300:CDS
AACTGTCAGAGTAATTGTCA+TGG 0.629228 7_1:-75117699 Msa1038300:CDS
TGATGTGATGGAGCTCCGGT+GGG 0.634768 7_1:-75116752 Msa1038300:three_prime_UTR
ATTGTAGATCAATGCAGCAA+TGG 0.636888 7_1:-75117191 Msa1038300:CDS
TAAACAGAGAAACAAACACA+GGG 0.637939 7_1:-75117916 Msa1038300:five_prime_UTR
ATAAACAGAGAAACAAACAC+AGG 0.641589 7_1:-75117917 Msa1038300:five_prime_UTR
ACGGAAACTCTTCCAGAGTG+AGG 0.645712 7_1:+75116717 None:intergenic
TACTATCAACCAGAGCAACA+TGG 0.648851 7_1:-75117627 Msa1038300:CDS
TGCAGCCAATACGGTTGGTG+TGG 0.651269 7_1:-75117747 Msa1038300:CDS
TTTAACAGAATCGACACAGA+TGG 0.651956 7_1:-75117143 Msa1038300:CDS
CCACAAGAAGCTTGACCACG+AGG 0.656609 7_1:+75117498 None:intergenic
AGAATCGACACAGATGGCAG+AGG 0.657142 7_1:-75117137 Msa1038300:CDS
ACACCAATGTTAATACCGCT+AGG 0.661884 7_1:+75117844 None:intergenic
AATTGTATTAGATTAGACAT+GGG 0.670498 7_1:-75117882 Msa1038300:exon
GCTCCGGTGGGATCACGGTG+CGG 0.677688 7_1:-75116740 Msa1038300:three_prime_UTR
CAATGCGGTAAACAAGCTGG+AGG 0.695826 7_1:-75117795 Msa1038300:CDS
CTTGACCACGAGGTCCAACA+GGG 0.697195 7_1:+75117508 None:intergenic
CGGACCACAGTATTCATCTG+TGG 0.698610 7_1:+75117722 None:intergenic
ATATACCCATGTGCATGCGG+CGG 0.709883 7_1:+75118032 None:intergenic
GCATCAATGATGGAGAACAG+AGG 0.711827 7_1:+75117044 None:intergenic
TCTATTTATGACTATGTACG+TGG 0.714653 7_1:-75116840 Msa1038300:three_prime_UTR
GATGGCAGAGGATACCAACA+GGG 0.745988 7_1:-75117125 Msa1038300:CDS
ACAACAGTTGCAGAACAATG+CGG 0.746680 7_1:-75117810 Msa1038300:CDS
TATTAATTACTACAGCACTG+AGG 0.756989 7_1:+75116991 None:intergenic
AAACAGAGAAACAAACACAG+GGG 0.780719 7_1:-75117915 Msa1038300:five_prime_UTR
AAAATTTGGAGTAAGCCTAG+CGG 0.781644 7_1:-75117859 Msa1038300:CDS
GGTACCACAGATGAATACTG+TGG 0.812966 7_1:-75117726 Msa1038300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAGAAAGTAATAAAAT+TGG + chr7_1:75117314-75117333 None:intergenic 10.0%
!! AATATAGAAAGTAATAAAAT+TGG + chr7_1:75117314-75117333 None:intergenic 10.0%
!! TAAAACATTAATTTAGACTT+TGG + chr7_1:75117356-75117375 None:intergenic 15.0%
!! TAAAACATTAATTTAGACTT+TGG + chr7_1:75117356-75117375 None:intergenic 15.0%
!! TCTTATTAAATTATTGCCAA+TGG - chr7_1:75116786-75116805 Msa1038300:three_prime_UTR 20.0%
!! CTCTGTTTATATAGAAAAAT+AGG + chr7_1:75116825-75116844 None:intergenic 20.0%
!! AAATTGTATTAGATTAGACA+TGG - chr7_1:75116869-75116888 Msa1038300:three_prime_UTR 20.0%
!! AATTGTATTAGATTAGACAT+GGG - chr7_1:75116870-75116889 Msa1038300:three_prime_UTR 20.0%
!! GGATTAGATATAAAATAGAT+AGG - chr7_1:75117382-75117401 Msa1038300:intron 20.0%
!! TCTTATTAAATTATTGCCAA+TGG - chr7_1:75116786-75116805 Msa1038300:three_prime_UTR 20.0%
!! CTCTGTTTATATAGAAAAAT+AGG + chr7_1:75116825-75116844 None:intergenic 20.0%
!! AAATTGTATTAGATTAGACA+TGG - chr7_1:75116869-75116888 Msa1038300:three_prime_UTR 20.0%
!! AATTGTATTAGATTAGACAT+GGG - chr7_1:75116870-75116889 Msa1038300:three_prime_UTR 20.0%
!! GGATTAGATATAAAATAGAT+AGG - chr7_1:75117382-75117401 Msa1038300:intron 20.0%
! ATTCTAGTCAACAATATAAC+AGG + chr7_1:75117959-75117978 None:intergenic 25.0%
! ATTCTAGTCAACAATATAAC+AGG + chr7_1:75117959-75117978 None:intergenic 25.0%
! TTTATGTCACGATATTGACT+AGG + chr7_1:75116762-75116781 None:intergenic 30.0%
TTATTGCCAATGGAAAATGA+TGG - chr7_1:75116796-75116815 Msa1038300:three_prime_UTR 30.0%
ATAAACAGAGAAACAAACAC+AGG - chr7_1:75116835-75116854 Msa1038300:three_prime_UTR 30.0%
TAAACAGAGAAACAAACACA+GGG - chr7_1:75116836-75116855 Msa1038300:three_prime_UTR 30.0%
AGATTAGACATGGGAAAATT+TGG - chr7_1:75116879-75116898 Msa1038300:three_prime_UTR 30.0%
TAAGTGCTTATTGTTCAACT+TGG - chr7_1:75117165-75117184 Msa1038300:CDS 30.0%
AAGTGCTTATTGTTCAACTT+GGG - chr7_1:75117166-75117185 Msa1038300:CDS 30.0%
TATTGTAAGCTATCAGTTTG+TGG - chr7_1:75117656-75117675 Msa1038300:CDS 30.0%
TTATTGTTCAGCATCAATGA+TGG + chr7_1:75117721-75117740 None:intergenic 30.0%
TATTAATTACTACAGCACTG+AGG + chr7_1:75117764-75117783 None:intergenic 30.0%
GTGCTGTAGTAATTAATAAG+TGG - chr7_1:75117766-75117785 Msa1038300:CDS 30.0%
TCTATTTATGACTATGTACG+TGG - chr7_1:75117912-75117931 Msa1038300:five_prime_UTR 30.0%
GAATATTCCAACCATAAAAG+TGG - chr7_1:75117975-75117994 Msa1038300:five_prime_UTR 30.0%
! TTTATGTCACGATATTGACT+AGG + chr7_1:75116762-75116781 None:intergenic 30.0%
TTATTGCCAATGGAAAATGA+TGG - chr7_1:75116796-75116815 Msa1038300:three_prime_UTR 30.0%
ATAAACAGAGAAACAAACAC+AGG - chr7_1:75116835-75116854 Msa1038300:three_prime_UTR 30.0%
TAAACAGAGAAACAAACACA+GGG - chr7_1:75116836-75116855 Msa1038300:three_prime_UTR 30.0%
AGATTAGACATGGGAAAATT+TGG - chr7_1:75116879-75116898 Msa1038300:three_prime_UTR 30.0%
TAAGTGCTTATTGTTCAACT+TGG - chr7_1:75117165-75117184 Msa1038300:CDS 30.0%
AAGTGCTTATTGTTCAACTT+GGG - chr7_1:75117166-75117185 Msa1038300:CDS 30.0%
TATTGTAAGCTATCAGTTTG+TGG - chr7_1:75117656-75117675 Msa1038300:CDS 30.0%
TTATTGTTCAGCATCAATGA+TGG + chr7_1:75117721-75117740 None:intergenic 30.0%
TATTAATTACTACAGCACTG+AGG + chr7_1:75117764-75117783 None:intergenic 30.0%
GTGCTGTAGTAATTAATAAG+TGG - chr7_1:75117766-75117785 Msa1038300:CDS 30.0%
TCTATTTATGACTATGTACG+TGG - chr7_1:75117912-75117931 Msa1038300:five_prime_UTR 30.0%
GAATATTCCAACCATAAAAG+TGG - chr7_1:75117975-75117994 Msa1038300:five_prime_UTR 30.0%
! AGGTAACCATCATTTTCCAT+TGG + chr7_1:75116805-75116824 None:intergenic 35.0%
AAACAGAGAAACAAACACAG+GGG - chr7_1:75116837-75116856 Msa1038300:three_prime_UTR 35.0%
! AAAATTTGGAGTAAGCCTAG+CGG - chr7_1:75116893-75116912 Msa1038300:three_prime_UTR 35.0%
TGACAATTACTCTGACAGTT+CGG + chr7_1:75117053-75117072 None:intergenic 35.0%
AACTGTCAGAGTAATTGTCA+TGG - chr7_1:75117053-75117072 Msa1038300:CDS 35.0%
! GAATGTAAATGTGATGATGC+AGG - chr7_1:75117500-75117519 Msa1038300:CDS 35.0%
ATTGTAGATCAATGCAGCAA+TGG - chr7_1:75117561-75117580 Msa1038300:CDS 35.0%
TTTAACAGAATCGACACAGA+TGG - chr7_1:75117609-75117628 Msa1038300:CDS 35.0%
TTACAATAAGATGTCCCTGT+TGG + chr7_1:75117644-75117663 None:intergenic 35.0%
AGTGGTTGTGTTTGTAACAA+AGG - chr7_1:75117784-75117803 Msa1038300:CDS 35.0%
!! ATCACATCAGACCACTTTTA+TGG + chr7_1:75117989-75118008 None:intergenic 35.0%
ATAAAAGTGGTCTGATGTGA+TGG - chr7_1:75117988-75118007 Msa1038300:five_prime_UTR 35.0%
! AGGTAACCATCATTTTCCAT+TGG + chr7_1:75116805-75116824 None:intergenic 35.0%
AAACAGAGAAACAAACACAG+GGG - chr7_1:75116837-75116856 Msa1038300:three_prime_UTR 35.0%
! AAAATTTGGAGTAAGCCTAG+CGG - chr7_1:75116893-75116912 Msa1038300:three_prime_UTR 35.0%
TGACAATTACTCTGACAGTT+CGG + chr7_1:75117053-75117072 None:intergenic 35.0%
AACTGTCAGAGTAATTGTCA+TGG - chr7_1:75117053-75117072 Msa1038300:CDS 35.0%
! GAATGTAAATGTGATGATGC+AGG - chr7_1:75117500-75117519 Msa1038300:CDS 35.0%
ATTGTAGATCAATGCAGCAA+TGG - chr7_1:75117561-75117580 Msa1038300:CDS 35.0%
TTTAACAGAATCGACACAGA+TGG - chr7_1:75117609-75117628 Msa1038300:CDS 35.0%
TTACAATAAGATGTCCCTGT+TGG + chr7_1:75117644-75117663 None:intergenic 35.0%
AGTGGTTGTGTTTGTAACAA+AGG - chr7_1:75117784-75117803 Msa1038300:CDS 35.0%
!! ATCACATCAGACCACTTTTA+TGG + chr7_1:75117989-75118008 None:intergenic 35.0%
ATAAAAGTGGTCTGATGTGA+TGG - chr7_1:75117988-75118007 Msa1038300:five_prime_UTR 35.0%
AAGCCTAGCGGTATTAACAT+TGG - chr7_1:75116905-75116924 Msa1038300:three_prime_UTR 40.0%
ACACCAATGTTAATACCGCT+AGG + chr7_1:75116911-75116930 None:intergenic 40.0%
ACAACAGTTGCAGAACAATG+CGG - chr7_1:75116942-75116961 Msa1038300:three_prime_UTR 40.0%
AACCTTTGTTGCAGCCAATA+CGG - chr7_1:75116996-75117015 Msa1038300:three_prime_UTR 40.0%
AGTAATTGTCATGGCAGTAG+TGG - chr7_1:75117062-75117081 Msa1038300:CDS 40.0%
TACTATCAACCAGAGCAACA+TGG - chr7_1:75117125-75117144 Msa1038300:CDS 40.0%
ATGCAAGCAAACCTTACTCA+TGG - chr7_1:75117189-75117208 Msa1038300:CDS 40.0%
!!! TATGGTTGGACTGCTTTTTG+TGG - chr7_1:75117221-75117240 Msa1038300:CDS 40.0%
!! TTCTTGTGGAAAGTGTCTCA+GGG - chr7_1:75117268-75117287 Msa1038300:intron 40.0%
ATGATGCAGGTGACAAATTC+AGG - chr7_1:75117513-75117532 Msa1038300:CDS 40.0%
TGATGCAGGTGACAAATTCA+GGG - chr7_1:75117514-75117533 Msa1038300:CDS 40.0%
TAGATCAATGCAGCAATGGA+GGG - chr7_1:75117565-75117584 Msa1038300:CDS 40.0%
! AGCTATCAGTTTGTGGATTG+TGG - chr7_1:75117663-75117682 Msa1038300:CDS 40.0%
! GCTATCAGTTTGTGGATTGT+GGG - chr7_1:75117664-75117683 Msa1038300:CDS 40.0%
! AATGATGGAGAACAGAGGAT+TGG + chr7_1:75117706-75117725 None:intergenic 40.0%
!! CATCAGACCACTTTTATGGT+TGG + chr7_1:75117985-75118004 None:intergenic 40.0%
AAGCCTAGCGGTATTAACAT+TGG - chr7_1:75116905-75116924 Msa1038300:three_prime_UTR 40.0%
ACACCAATGTTAATACCGCT+AGG + chr7_1:75116911-75116930 None:intergenic 40.0%
ACAACAGTTGCAGAACAATG+CGG - chr7_1:75116942-75116961 Msa1038300:three_prime_UTR 40.0%
AACCTTTGTTGCAGCCAATA+CGG - chr7_1:75116996-75117015 Msa1038300:three_prime_UTR 40.0%
AGTAATTGTCATGGCAGTAG+TGG - chr7_1:75117062-75117081 Msa1038300:CDS 40.0%
TACTATCAACCAGAGCAACA+TGG - chr7_1:75117125-75117144 Msa1038300:CDS 40.0%
ATGCAAGCAAACCTTACTCA+TGG - chr7_1:75117189-75117208 Msa1038300:CDS 40.0%
!!! TATGGTTGGACTGCTTTTTG+TGG - chr7_1:75117221-75117240 Msa1038300:CDS 40.0%
!! TTCTTGTGGAAAGTGTCTCA+GGG - chr7_1:75117268-75117287 Msa1038300:intron 40.0%
ATGATGCAGGTGACAAATTC+AGG - chr7_1:75117513-75117532 Msa1038300:CDS 40.0%
TGATGCAGGTGACAAATTCA+GGG - chr7_1:75117514-75117533 Msa1038300:CDS 40.0%
TAGATCAATGCAGCAATGGA+GGG - chr7_1:75117565-75117584 Msa1038300:CDS 40.0%
! AGCTATCAGTTTGTGGATTG+TGG - chr7_1:75117663-75117682 Msa1038300:CDS 40.0%
! GCTATCAGTTTGTGGATTGT+GGG - chr7_1:75117664-75117683 Msa1038300:CDS 40.0%
! AATGATGGAGAACAGAGGAT+TGG + chr7_1:75117706-75117725 None:intergenic 40.0%
!! CATCAGACCACTTTTATGGT+TGG + chr7_1:75117985-75118004 None:intergenic 40.0%
GACATATACCCATGTGCATG+CGG + chr7_1:75116726-75116745 None:intergenic 45.0%
! GTCACGATATTGACTAGGCA+TGG + chr7_1:75116757-75116776 None:intergenic 45.0%
GAACAATGCGGTAAACAAGC+TGG - chr7_1:75116954-75116973 Msa1038300:three_prime_UTR 45.0%
TGGCTGCAACAAAGGTTGTT+TGG + chr7_1:75116993-75117012 None:intergenic 45.0%
!! AACCGTATTGGCTGCAACAA+AGG + chr7_1:75117001-75117020 None:intergenic 45.0%
TTTGTTGCAGCCAATACGGT+TGG - chr7_1:75117000-75117019 Msa1038300:three_prime_UTR 45.0%
GGTACCACAGATGAATACTG+TGG - chr7_1:75117026-75117045 Msa1038300:exon 45.0%
AATTGTCATGGCAGTAGTGG+TGG - chr7_1:75117065-75117084 Msa1038300:CDS 45.0%
ATCAACCAGAGCAACATGGA+TGG - chr7_1:75117129-75117148 Msa1038300:CDS 45.0%
TCAACCAGAGCAACATGGAT+GGG - chr7_1:75117130-75117149 Msa1038300:CDS 45.0%
! TACTTGCTACGCCATGAGTA+AGG + chr7_1:75117203-75117222 None:intergenic 45.0%
TACTCATGGCGTAGCAAGTA+TGG - chr7_1:75117203-75117222 Msa1038300:CDS 45.0%
!! CTTCTTGTGGAAAGTGTCTC+AGG - chr7_1:75117267-75117286 Msa1038300:intron 45.0%
GTAGATCAATGCAGCAATGG+AGG - chr7_1:75117564-75117583 Msa1038300:CDS 45.0%
! GCATCAATGATGGAGAACAG+AGG + chr7_1:75117711-75117730 None:intergenic 45.0%
GACATATACCCATGTGCATG+CGG + chr7_1:75116726-75116745 None:intergenic 45.0%
! GTCACGATATTGACTAGGCA+TGG + chr7_1:75116757-75116776 None:intergenic 45.0%
GAACAATGCGGTAAACAAGC+TGG - chr7_1:75116954-75116973 Msa1038300:three_prime_UTR 45.0%
TGGCTGCAACAAAGGTTGTT+TGG + chr7_1:75116993-75117012 None:intergenic 45.0%
!! AACCGTATTGGCTGCAACAA+AGG + chr7_1:75117001-75117020 None:intergenic 45.0%
TTTGTTGCAGCCAATACGGT+TGG - chr7_1:75117000-75117019 Msa1038300:three_prime_UTR 45.0%
GGTACCACAGATGAATACTG+TGG - chr7_1:75117026-75117045 Msa1038300:exon 45.0%
AATTGTCATGGCAGTAGTGG+TGG - chr7_1:75117065-75117084 Msa1038300:CDS 45.0%
ATCAACCAGAGCAACATGGA+TGG - chr7_1:75117129-75117148 Msa1038300:CDS 45.0%
TCAACCAGAGCAACATGGAT+GGG - chr7_1:75117130-75117149 Msa1038300:CDS 45.0%
! TACTTGCTACGCCATGAGTA+AGG + chr7_1:75117203-75117222 None:intergenic 45.0%
TACTCATGGCGTAGCAAGTA+TGG - chr7_1:75117203-75117222 Msa1038300:CDS 45.0%
!! CTTCTTGTGGAAAGTGTCTC+AGG - chr7_1:75117267-75117286 Msa1038300:intron 45.0%
GTAGATCAATGCAGCAATGG+AGG - chr7_1:75117564-75117583 Msa1038300:CDS 45.0%
! GCATCAATGATGGAGAACAG+AGG + chr7_1:75117711-75117730 None:intergenic 45.0%
!!! TAAATTAATGTTTTAAAAAT+AGG - chr7_1:75117361-75117380 Msa1038300:intron 5.0%
!!! TAAATTAATGTTTTAAAAAT+AGG - chr7_1:75117361-75117380 Msa1038300:intron 5.0%
ATATACCCATGTGCATGCGG+CGG + chr7_1:75116723-75116742 None:intergenic 50.0%
CAATGCGGTAAACAAGCTGG+AGG - chr7_1:75116957-75116976 Msa1038300:three_prime_UTR 50.0%
TGGTACCACACCAACCGTAT+TGG + chr7_1:75117013-75117032 None:intergenic 50.0%
CGGACCACAGTATTCATCTG+TGG + chr7_1:75117033-75117052 None:intergenic 50.0%
AAGTCCCATCCATGTTGCTC+TGG + chr7_1:75117137-75117156 None:intergenic 50.0%
CATGGCGTAGCAAGTATGGT+TGG - chr7_1:75117207-75117226 Msa1038300:CDS 50.0%
!!! ACTGCTTTTTGTGGCCCTGT+TGG - chr7_1:75117230-75117249 Msa1038300:CDS 50.0%
GCAGGTGACAAATTCAGGGA+CGG - chr7_1:75117518-75117537 Msa1038300:CDS 50.0%
CAGGTGACAAATTCAGGGAC+GGG - chr7_1:75117519-75117538 Msa1038300:CDS 50.0%
TCAATGCAGCAATGGAGGGT+TGG - chr7_1:75117569-75117588 Msa1038300:CDS 50.0%
CAGCAATGGAGGGTTGGATT+TGG - chr7_1:75117575-75117594 Msa1038300:CDS 50.0%
GGAGGGTTGGATTTGGATGT+AGG - chr7_1:75117582-75117601 Msa1038300:CDS 50.0%
AGAATCGACACAGATGGCAG+AGG - chr7_1:75117615-75117634 Msa1038300:CDS 50.0%
AGATGGCAGAGGATACCAAC+AGG - chr7_1:75117626-75117645 Msa1038300:CDS 50.0%
GATGGCAGAGGATACCAACA+GGG - chr7_1:75117627-75117646 Msa1038300:CDS 50.0%
! TTACTACAGCACTGAGGCAG+AGG + chr7_1:75117758-75117777 None:intergenic 50.0%
ATATACCCATGTGCATGCGG+CGG + chr7_1:75116723-75116742 None:intergenic 50.0%
CAATGCGGTAAACAAGCTGG+AGG - chr7_1:75116957-75116976 Msa1038300:three_prime_UTR 50.0%
TGGTACCACACCAACCGTAT+TGG + chr7_1:75117013-75117032 None:intergenic 50.0%
CGGACCACAGTATTCATCTG+TGG + chr7_1:75117033-75117052 None:intergenic 50.0%
AAGTCCCATCCATGTTGCTC+TGG + chr7_1:75117137-75117156 None:intergenic 50.0%
CATGGCGTAGCAAGTATGGT+TGG - chr7_1:75117207-75117226 Msa1038300:CDS 50.0%
!!! ACTGCTTTTTGTGGCCCTGT+TGG - chr7_1:75117230-75117249 Msa1038300:CDS 50.0%
GCAGGTGACAAATTCAGGGA+CGG - chr7_1:75117518-75117537 Msa1038300:CDS 50.0%
CAGGTGACAAATTCAGGGAC+GGG - chr7_1:75117519-75117538 Msa1038300:CDS 50.0%
TCAATGCAGCAATGGAGGGT+TGG - chr7_1:75117569-75117588 Msa1038300:CDS 50.0%
CAGCAATGGAGGGTTGGATT+TGG - chr7_1:75117575-75117594 Msa1038300:CDS 50.0%
GGAGGGTTGGATTTGGATGT+AGG - chr7_1:75117582-75117601 Msa1038300:CDS 50.0%
AGAATCGACACAGATGGCAG+AGG - chr7_1:75117615-75117634 Msa1038300:CDS 50.0%
AGATGGCAGAGGATACCAAC+AGG - chr7_1:75117626-75117645 Msa1038300:CDS 50.0%
GATGGCAGAGGATACCAACA+GGG - chr7_1:75117627-75117646 Msa1038300:CDS 50.0%
! TTACTACAGCACTGAGGCAG+AGG + chr7_1:75117758-75117777 None:intergenic 50.0%
! TGCAGCCAATACGGTTGGTG+TGG - chr7_1:75117005-75117024 Msa1038300:three_prime_UTR 55.0%
TGTCATGGCAGTAGTGGTGG+TGG - chr7_1:75117068-75117087 Msa1038300:CDS 55.0%
CTTGACCACGAGGTCCAACA+GGG + chr7_1:75117247-75117266 None:intergenic 55.0%
CCACAAGAAGCTTGACCACG+AGG + chr7_1:75117257-75117276 None:intergenic 55.0%
CCTCGTGGTCAAGCTTCTTG+TGG - chr7_1:75117254-75117273 Msa1038300:intron 55.0%
! AAATTCAGGGACGGGAGCAC+AGG - chr7_1:75117527-75117546 Msa1038300:CDS 55.0%
CTGAGGCAGAGGAAGCAGAT+TGG + chr7_1:75117747-75117766 None:intergenic 55.0%
! GGTCTGATGTGATGGAGCTC+CGG - chr7_1:75117996-75118015 Msa1038300:five_prime_UTR 55.0%
! TGATGTGATGGAGCTCCGGT+GGG - chr7_1:75118000-75118019 Msa1038300:five_prime_UTR 55.0%
TATACCCATGTGCATGCGGC+GGG + chr7_1:75116722-75116741 None:intergenic 55.0%
! TGCAGCCAATACGGTTGGTG+TGG - chr7_1:75117005-75117024 Msa1038300:three_prime_UTR 55.0%
TGTCATGGCAGTAGTGGTGG+TGG - chr7_1:75117068-75117087 Msa1038300:CDS 55.0%
CTTGACCACGAGGTCCAACA+GGG + chr7_1:75117247-75117266 None:intergenic 55.0%
CCACAAGAAGCTTGACCACG+AGG + chr7_1:75117257-75117276 None:intergenic 55.0%
CCTCGTGGTCAAGCTTCTTG+TGG - chr7_1:75117254-75117273 Msa1038300:intron 55.0%
! AAATTCAGGGACGGGAGCAC+AGG - chr7_1:75117527-75117546 Msa1038300:CDS 55.0%
CTGAGGCAGAGGAAGCAGAT+TGG + chr7_1:75117747-75117766 None:intergenic 55.0%
! GGTCTGATGTGATGGAGCTC+CGG - chr7_1:75117996-75118015 Msa1038300:five_prime_UTR 55.0%
! TGATGTGATGGAGCTCCGGT+GGG - chr7_1:75118000-75118019 Msa1038300:five_prime_UTR 55.0%
GCTTGACCACGAGGTCCAAC+AGG + chr7_1:75117248-75117267 None:intergenic 60.0%
! CTGATGTGATGGAGCTCCGG+TGG - chr7_1:75117999-75118018 Msa1038300:five_prime_UTR 60.0%
ATGGAGCTCCGGTGGGATCA+CGG - chr7_1:75118007-75118026 Msa1038300:five_prime_UTR 60.0%
GCTTGACCACGAGGTCCAAC+AGG + chr7_1:75117248-75117267 None:intergenic 60.0%
! CTGATGTGATGGAGCTCCGG+TGG - chr7_1:75117999-75118018 Msa1038300:five_prime_UTR 60.0%
ATGGAGCTCCGGTGGGATCA+CGG - chr7_1:75118007-75118026 Msa1038300:five_prime_UTR 60.0%
TGTGGCCCTGTTGGACCTCG+TGG - chr7_1:75117239-75117258 Msa1038300:intron 65.0%
ATCACGGTGCGGCCTCACTC+TGG - chr7_1:75118023-75118042 Msa1038300:five_prime_UTR 65.0%
TGTGGCCCTGTTGGACCTCG+TGG - chr7_1:75117239-75117258 Msa1038300:intron 65.0%
ATCACGGTGCGGCCTCACTC+TGG - chr7_1:75118023-75118042 Msa1038300:five_prime_UTR 65.0%
GCTCCGGTGGGATCACGGTG+CGG - chr7_1:75118012-75118031 Msa1038300:five_prime_UTR 70.0%
AGGCCGCACCGTGATCCCAC+CGG + chr7_1:75118018-75118037 None:intergenic 70.0%
GCTCCGGTGGGATCACGGTG+CGG - chr7_1:75118012-75118031 Msa1038300:five_prime_UTR 70.0%
AGGCCGCACCGTGATCCCAC+CGG + chr7_1:75118018-75118037 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 75116720 75118054 75116720 ID=Msa1038300;Name=Msa1038300
chr7_1 mRNA 75116720 75118054 75116720 ID=Msa1038300-mRNA-1;Parent=Msa1038300;Name=Msa1038300-mRNA-1;_AED=0.01;_eAED=0.01;_QI=168|1|1|1|1|1|2|314|206
chr7_1 exon 75116720 75117252 75116720 ID=Msa1038300-mRNA-1:exon:19122;Parent=Msa1038300-mRNA-1
chr7_1 exon 75117485 75118054 75117485 ID=Msa1038300-mRNA-1:exon:19121;Parent=Msa1038300-mRNA-1
chr7_1 five_prime_UTR 75117887 75118054 75117887 ID=Msa1038300-mRNA-1:five_prime_utr;Parent=Msa1038300-mRNA-1
chr7_1 CDS 75117485 75117886 75117485 ID=Msa1038300-mRNA-1:cds;Parent=Msa1038300-mRNA-1
chr7_1 CDS 75117034 75117252 75117034 ID=Msa1038300-mRNA-1:cds;Parent=Msa1038300-mRNA-1
chr7_1 three_prime_UTR 75116720 75117033 75116720 ID=Msa1038300-mRNA-1:three_prime_utr;Parent=Msa1038300-mRNA-1
Gene Sequence

>Msa1038300

ATGGGAAAATTTGGAGTAAGCCTAGCGGTATTAACATTGGTGTGTTTGACTGCAACAACAGTTGCAGAACAATGCGGTAAACAAGCTGGAGGAAAAACATGTCCAAACAACCTTTGTTGCAGCCAATACGGTTGGTGTGGTACCACAGATGAATACTGTGGTCCGAACTGTCAGAGTAATTGTCATGGCAGTAGTGGTGGTGGTGAAAGTGCTTCTAATGTTCGTGCTACTTATCATTACTATCAACCAGAGCAACATGGATGGGACTTAAACGCTGTAAGTGCTTATTGTTCAACTTGGGATGCAAGCAAACCTTACTCATGGCGTAGCAAGTATGGTTGGACTGCTTTTTGTGGCCCTGTTGGACCTCGTGGTCAAGCTTCTTGTGGAAAGTGTCTCAGGGTGACAAATTCAGGGACGGGAGCACAGGAAACAGTGAGAATTGTAGATCAATGCAGCAATGGAGGGTTGGATTTGGATGTAGGAGTGTTTAACAGAATCGACACAGATGGCAGAGGATACCAACAGGGACATCTTATTGTAAGCTATCAGTTTGTGGATTGTGGGAATGAGCTAGACATGACCAATCCTCTGTTCTCCATCATTGATGCTGAACAATAA
Protein sequence

>Msa1038300

MGKFGVSLAVLTLVCLTATTVAEQCGKQAGGKTCPNNLCCSQYGWCGTTDEYCGPNCQSNCHGSSGGGESASNVRATYHYYQPEQHGWDLNAVSAYCSTWDASKPYSWRSKYGWTAFCGPVGPRGQASCGKCLRVTNSGTGAQETVRIVDQCSNGGLDLDVGVFNRIDTDGRGYQQGHLIVSYQFVDCGNELDMTNPLFSIIDAEQ*