AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449580 MtrunA17_Chr7g0274181 93.689 206 9 1 1 206 1 202 7.06e-136 379
Msa1449580 MtrunA17_Chr1g0190671 71.901 121 34 0 68 188 24 144 4.54e-64 194
Msa1449580 MtrunA17_Chr1g0190661 71.074 121 35 0 68 188 24 144 2.01e-63 193
Msa1449580 MtrunA17_Chr1g0190641 70.000 120 36 0 71 190 22 141 3.28e-63 192
Msa1449580 MtrunA17_Chr1g0190651 70.732 123 36 0 68 190 24 146 9.26e-63 191
Msa1449580 MtrunA17_Chr6g0484021 50.000 154 60 2 61 197 35 188 4.63e-43 152
Msa1449580 MtrunA17_Chr6g0483971 47.692 130 64 2 61 188 15 142 9.82e-37 130
Msa1449580 MtrunA17_Chr6g0484031 47.445 137 61 2 61 190 15 147 1.86e-33 125
Msa1449580 MtrunA17_Chr6g0484041 44.853 136 58 3 68 188 34 167 5.72e-32 121
Msa1449580 MtrunA17_Chr3g0145981 51.515 66 32 0 1 66 24 89 6.38e-17 77.8
Msa1449580 MtrunA17_Chr8g0370831 68.293 41 13 0 22 62 22 62 5.80e-13 66.2
Msa1449580 MtrunA17_Chr8g0370851 50.000 56 28 0 6 61 7 62 6.71e-12 63.2
Msa1449580 MtrunA17_Chr8g0370821 67.500 40 13 0 22 61 33 72 1.12e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449580 AT3G04720.1 71.292 209 52 3 3 203 2 210 3.70e-97 281
Msa1449580 AT3G12500.1 68.293 41 12 1 22 61 33 73 8.62e-12 63.5

Find 49 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTAGACATGGGAAAATT+TGG 0.218092 tig0032655:-1076 Msa1449580:exon
TATTGCCAATGGGAAAATAA+TGG 0.341893 tig0032655:-1158 Msa1449580:five_prime_UTR
TACTCATGGCGTAGCAAGTA+TGG 0.353266 tig0032655:-752 Msa1449580:CDS
CTTCTTGTGGAAAGTGTCTC+AGG 0.353594 tig0032655:-688 Msa1449580:CDS
TGGCTGCAACAAAGGTTGTT+TGG 0.365069 tig0032655:+965 None:intergenic
GCTTGACCACGAGGTCCAAC+AGG 0.397549 tig0032655:+710 None:intergenic
AAGTCCCATCCATGTTGCTC+TGG 0.422201 tig0032655:+821 None:intergenic
CTTATTAAATTATTGCCAAT+GGG 0.435162 tig0032655:-1168 Msa1449580:five_prime_UTR
TCTTATTAAATTATTGCCAA+TGG 0.448300 tig0032655:-1169 Msa1449580:five_prime_UTR
AACCTTTGTTGCAGCCAATA+CGG 0.476377 tig0032655:-959 Msa1449580:CDS
TACTTGCTACGCCATGAGTA+AGG 0.479661 tig0032655:+755 None:intergenic
AACCGTATTGGCTGCAACAA+AGG 0.489204 tig0032655:+957 None:intergenic
GAACAATGCGGTAAACAAGC+TGG 0.511319 tig0032655:-1001 Msa1449580:CDS
TGGTACCACACCAACCGTAT+TGG 0.526780 tig0032655:+945 None:intergenic
TAAGTGCTTATTGTTCAACT+TGG 0.529463 tig0032655:-790 Msa1449580:CDS
TGACAATTACTCTGACAGTT+CGG 0.538086 tig0032655:+905 None:intergenic
CCTCGTGGTCAAGCTTCTTG+TGG 0.556286 tig0032655:-701 Msa1449580:CDS
AAGTGCTTATTGTTCAACTT+GGG 0.564174 tig0032655:-789 Msa1449580:CDS
TTTGTTGCAGCCAATACGGT+TGG 0.578744 tig0032655:-955 Msa1449580:CDS
GACATATACCCATGTGCATG+CGG 0.585883 tig0032655:+1232 None:intergenic
ATGCAAGCAAACCTTACTCA+TGG 0.586811 tig0032655:-766 Msa1449580:CDS
ATCAACCAGAGCAACATGGA+TGG 0.587569 tig0032655:-826 Msa1449580:CDS
TCAACCAGAGCAACATGGAT+GGG 0.598724 tig0032655:-825 Msa1449580:CDS
AAATTGTATTAGATTAGACA+TGG 0.600324 tig0032655:-1086 Msa1449580:exon
CATGGCGTAGCAAGTATGGT+TGG 0.600676 tig0032655:-748 Msa1449580:CDS
TTTATGTCACGATATTGACT+AGG 0.609065 tig0032655:+1196 None:intergenic
AGTAATTGTCATGGCAGTAG+TGG 0.614410 tig0032655:-893 Msa1449580:CDS
GTCACGATATTGACTAGGCA+TGG 0.614770 tig0032655:+1201 None:intergenic
TGTGGCCCTGTTGGACCTCG+TGG 0.621769 tig0032655:-716 Msa1449580:CDS
TGTCATGGCAGTAGTGGTGG+TGG 0.621989 tig0032655:-887 Msa1449580:CDS
TTCTTGTGGAAAGTGTCTCA+GGG 0.622227 tig0032655:-687 Msa1449580:intron
AATTGTCATGGCAGTAGTGG+TGG 0.624496 tig0032655:-890 Msa1449580:CDS
AAGCCTAGCGGTATTAACAT+TGG 0.627981 tig0032655:-1050 Msa1449580:CDS
AACTGTCAGAGTAATTGTCA+TGG 0.629228 tig0032655:-902 Msa1449580:CDS
TAAACAGAGAAACAAACACA+GGG 0.637939 tig0032655:-1118 Msa1449580:five_prime_UTR
ATAAACAGAGAAACAAACAC+AGG 0.641589 tig0032655:-1119 Msa1449580:five_prime_UTR
TACTATCAACCAGAGCAACA+TGG 0.648851 tig0032655:-830 Msa1449580:CDS
TGCAGCCAATACGGTTGGTG+TGG 0.651269 tig0032655:-950 Msa1449580:CDS
CCACAAGAAGCTTGACCACG+AGG 0.656609 tig0032655:+701 None:intergenic
ACACCAATGTTAATACCGCT+AGG 0.661884 tig0032655:+1047 None:intergenic
AATTGTATTAGATTAGACAT+GGG 0.670498 tig0032655:-1085 Msa1449580:exon
CAATGCGGTAAACAAGCTGG+AGG 0.695826 tig0032655:-998 Msa1449580:CDS
CTTGACCACGAGGTCCAACA+GGG 0.697195 tig0032655:+711 None:intergenic
CGGACCACAGTATTCATCTG+TGG 0.698610 tig0032655:+925 None:intergenic
ATATACCCATGTGCATGCGG+CGG 0.709883 tig0032655:+1235 None:intergenic
ACAACAGTTGCAGAACAATG+CGG 0.746680 tig0032655:-1013 Msa1449580:CDS
AAACAGAGAAACAAACACAG+GGG 0.780719 tig0032655:-1117 Msa1449580:five_prime_UTR
AAAATTTGGAGTAAGCCTAG+CGG 0.781644 tig0032655:-1062 Msa1449580:CDS
GGTACCACAGATGAATACTG+TGG 0.812966 tig0032655:-929 Msa1449580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATAAAAAGGAATATAAT+TGG + tig0032655:595-614 None:intergenic 15.0%
!! TAGAGTAAATATAGATAAAA+AGG + tig0032655:606-625 None:intergenic 15.0%
!!! TACTCTAATGTTTTAAAAAT+AGG - tig0032655:619-638 Msa1449580:intron 15.0%
!! TAGATAAAAAGGAATATAAT+TGG + tig0032655:595-614 None:intergenic 15.0%
!! TAGAGTAAATATAGATAAAA+AGG + tig0032655:606-625 None:intergenic 15.0%
!!! TACTCTAATGTTTTAAAAAT+AGG - tig0032655:619-638 Msa1449580:intron 15.0%
!! TCTTATTAAATTATTGCCAA+TGG - tig0032655:67-86 Msa1449580:three_prime_UTR 20.0%
!! CTTATTAAATTATTGCCAAT+GGG - tig0032655:68-87 Msa1449580:three_prime_UTR 20.0%
!! CTCTGTTTATATAGAAAAAT+AGG + tig0032655:107-126 None:intergenic 20.0%
!! AAATTGTATTAGATTAGACA+TGG - tig0032655:150-169 Msa1449580:three_prime_UTR 20.0%
!! AATTGTATTAGATTAGACAT+GGG - tig0032655:151-170 Msa1449580:three_prime_UTR 20.0%
!! TCTTATTAAATTATTGCCAA+TGG - tig0032655:67-86 Msa1449580:three_prime_UTR 20.0%
!! CTTATTAAATTATTGCCAAT+GGG - tig0032655:68-87 Msa1449580:three_prime_UTR 20.0%
!! CTCTGTTTATATAGAAAAAT+AGG + tig0032655:107-126 None:intergenic 20.0%
!! AAATTGTATTAGATTAGACA+TGG - tig0032655:150-169 Msa1449580:three_prime_UTR 20.0%
!! AATTGTATTAGATTAGACAT+GGG - tig0032655:151-170 Msa1449580:three_prime_UTR 20.0%
! CAATTGAACTATATATGATC+TGG + tig0032655:662-681 None:intergenic 25.0%
! ATTCTAGTCAACAATATAAC+AGG + tig0032655:1194-1213 None:intergenic 25.0%
! CAATTGAACTATATATGATC+TGG + tig0032655:662-681 None:intergenic 25.0%
! ATTCTAGTCAACAATATAAC+AGG + tig0032655:1194-1213 None:intergenic 25.0%
! TTTATGTCACGATATTGACT+AGG + tig0032655:43-62 None:intergenic 30.0%
TATTGCCAATGGGAAAATAA+TGG - tig0032655:78-97 Msa1449580:three_prime_UTR 30.0%
ATAAACAGAGAAACAAACAC+AGG - tig0032655:117-136 Msa1449580:three_prime_UTR 30.0%
TAAACAGAGAAACAAACACA+GGG - tig0032655:118-137 Msa1449580:three_prime_UTR 30.0%
AGATTAGACATGGGAAAATT+TGG - tig0032655:160-179 Msa1449580:three_prime_UTR 30.0%
TAAGTGCTTATTGTTCAACT+TGG - tig0032655:446-465 Msa1449580:CDS 30.0%
AAGTGCTTATTGTTCAACTT+GGG - tig0032655:447-466 Msa1449580:CDS 30.0%
TATTGTAAGCTATCAGTTTG+TGG - tig0032655:891-910 Msa1449580:CDS 30.0%
TTATTGTTCAGCATCAATGA+TGG + tig0032655:956-975 None:intergenic 30.0%
TATTAATTACTACAGCACTG+AGG + tig0032655:999-1018 None:intergenic 30.0%
GTGCTGTAGTAATTAATAAG+TGG - tig0032655:1001-1020 Msa1449580:CDS 30.0%
TCTATTTATGACTATGTACG+TGG - tig0032655:1147-1166 Msa1449580:five_prime_UTR 30.0%
GAATATTCCAACCATAAAAG+TGG - tig0032655:1210-1229 Msa1449580:five_prime_UTR 30.0%
! TTTATGTCACGATATTGACT+AGG + tig0032655:43-62 None:intergenic 30.0%
TATTGCCAATGGGAAAATAA+TGG - tig0032655:78-97 Msa1449580:three_prime_UTR 30.0%
ATAAACAGAGAAACAAACAC+AGG - tig0032655:117-136 Msa1449580:three_prime_UTR 30.0%
TAAACAGAGAAACAAACACA+GGG - tig0032655:118-137 Msa1449580:three_prime_UTR 30.0%
AGATTAGACATGGGAAAATT+TGG - tig0032655:160-179 Msa1449580:three_prime_UTR 30.0%
TAAGTGCTTATTGTTCAACT+TGG - tig0032655:446-465 Msa1449580:CDS 30.0%
AAGTGCTTATTGTTCAACTT+GGG - tig0032655:447-466 Msa1449580:CDS 30.0%
TATTGTAAGCTATCAGTTTG+TGG - tig0032655:891-910 Msa1449580:CDS 30.0%
TTATTGTTCAGCATCAATGA+TGG + tig0032655:956-975 None:intergenic 30.0%
TATTAATTACTACAGCACTG+AGG + tig0032655:999-1018 None:intergenic 30.0%
GTGCTGTAGTAATTAATAAG+TGG - tig0032655:1001-1020 Msa1449580:CDS 30.0%
TCTATTTATGACTATGTACG+TGG - tig0032655:1147-1166 Msa1449580:five_prime_UTR 30.0%
GAATATTCCAACCATAAAAG+TGG - tig0032655:1210-1229 Msa1449580:five_prime_UTR 30.0%
! GGTAACCATTATTTTCCCAT+TGG + tig0032655:86-105 None:intergenic 35.0%
AAACAGAGAAACAAACACAG+GGG - tig0032655:119-138 Msa1449580:three_prime_UTR 35.0%
! AAAATTTGGAGTAAGCCTAG+CGG - tig0032655:174-193 Msa1449580:three_prime_UTR 35.0%
TGACAATTACTCTGACAGTT+CGG + tig0032655:334-353 None:intergenic 35.0%
AACTGTCAGAGTAATTGTCA+TGG - tig0032655:334-353 Msa1449580:CDS 35.0%
! GAATGTAAATGTGATGATGC+AGG - tig0032655:735-754 Msa1449580:CDS 35.0%
ATTGTAGATCAATGCAGCAA+TGG - tig0032655:796-815 Msa1449580:CDS 35.0%
TTTAACAGAATCGACACAGA+TGG - tig0032655:844-863 Msa1449580:CDS 35.0%
TTACAATAAGATGTCCCTGT+TGG + tig0032655:879-898 None:intergenic 35.0%
AGTGGTTGTGTTTGTAACAA+AGG - tig0032655:1019-1038 Msa1449580:CDS 35.0%
!! ATCACATCAGACCACTTTTA+TGG + tig0032655:1224-1243 None:intergenic 35.0%
ATAAAAGTGGTCTGATGTGA+TGG - tig0032655:1223-1242 Msa1449580:five_prime_UTR 35.0%
! GGTAACCATTATTTTCCCAT+TGG + tig0032655:86-105 None:intergenic 35.0%
AAACAGAGAAACAAACACAG+GGG - tig0032655:119-138 Msa1449580:three_prime_UTR 35.0%
! AAAATTTGGAGTAAGCCTAG+CGG - tig0032655:174-193 Msa1449580:three_prime_UTR 35.0%
TGACAATTACTCTGACAGTT+CGG + tig0032655:334-353 None:intergenic 35.0%
AACTGTCAGAGTAATTGTCA+TGG - tig0032655:334-353 Msa1449580:CDS 35.0%
! GAATGTAAATGTGATGATGC+AGG - tig0032655:735-754 Msa1449580:CDS 35.0%
ATTGTAGATCAATGCAGCAA+TGG - tig0032655:796-815 Msa1449580:CDS 35.0%
TTTAACAGAATCGACACAGA+TGG - tig0032655:844-863 Msa1449580:CDS 35.0%
TTACAATAAGATGTCCCTGT+TGG + tig0032655:879-898 None:intergenic 35.0%
AGTGGTTGTGTTTGTAACAA+AGG - tig0032655:1019-1038 Msa1449580:CDS 35.0%
!! ATCACATCAGACCACTTTTA+TGG + tig0032655:1224-1243 None:intergenic 35.0%
ATAAAAGTGGTCTGATGTGA+TGG - tig0032655:1223-1242 Msa1449580:five_prime_UTR 35.0%
AAGCCTAGCGGTATTAACAT+TGG - tig0032655:186-205 Msa1449580:three_prime_UTR 40.0%
ACACCAATGTTAATACCGCT+AGG + tig0032655:192-211 None:intergenic 40.0%
ACAACAGTTGCAGAACAATG+CGG - tig0032655:223-242 Msa1449580:three_prime_UTR 40.0%
AACCTTTGTTGCAGCCAATA+CGG - tig0032655:277-296 Msa1449580:exon 40.0%
AGTAATTGTCATGGCAGTAG+TGG - tig0032655:343-362 Msa1449580:CDS 40.0%
TACTATCAACCAGAGCAACA+TGG - tig0032655:406-425 Msa1449580:CDS 40.0%
ATGCAAGCAAACCTTACTCA+TGG - tig0032655:470-489 Msa1449580:CDS 40.0%
!!! TATGGTTGGACTGCTTTTTG+TGG - tig0032655:502-521 Msa1449580:intron 40.0%
!! TTCTTGTGGAAAGTGTCTCA+GGG - tig0032655:549-568 Msa1449580:intron 40.0%
ATGATGCAGGTGACAAATTC+AGG - tig0032655:748-767 Msa1449580:CDS 40.0%
TGATGCAGGTGACAAATTCA+GGG - tig0032655:749-768 Msa1449580:CDS 40.0%
TAGATCAATGCAGCAATGGA+GGG - tig0032655:800-819 Msa1449580:CDS 40.0%
! AGCTATCAGTTTGTGGATTG+TGG - tig0032655:898-917 Msa1449580:CDS 40.0%
! GCTATCAGTTTGTGGATTGT+GGG - tig0032655:899-918 Msa1449580:CDS 40.0%
! AATGATGGAGAACAGAGGAT+TGG + tig0032655:941-960 None:intergenic 40.0%
!! CATCAGACCACTTTTATGGT+TGG + tig0032655:1220-1239 None:intergenic 40.0%
AAGCCTAGCGGTATTAACAT+TGG - tig0032655:186-205 Msa1449580:three_prime_UTR 40.0%
ACACCAATGTTAATACCGCT+AGG + tig0032655:192-211 None:intergenic 40.0%
ACAACAGTTGCAGAACAATG+CGG - tig0032655:223-242 Msa1449580:three_prime_UTR 40.0%
AACCTTTGTTGCAGCCAATA+CGG - tig0032655:277-296 Msa1449580:exon 40.0%
AGTAATTGTCATGGCAGTAG+TGG - tig0032655:343-362 Msa1449580:CDS 40.0%
TACTATCAACCAGAGCAACA+TGG - tig0032655:406-425 Msa1449580:CDS 40.0%
ATGCAAGCAAACCTTACTCA+TGG - tig0032655:470-489 Msa1449580:CDS 40.0%
!!! TATGGTTGGACTGCTTTTTG+TGG - tig0032655:502-521 Msa1449580:intron 40.0%
!! TTCTTGTGGAAAGTGTCTCA+GGG - tig0032655:549-568 Msa1449580:intron 40.0%
ATGATGCAGGTGACAAATTC+AGG - tig0032655:748-767 Msa1449580:CDS 40.0%
TGATGCAGGTGACAAATTCA+GGG - tig0032655:749-768 Msa1449580:CDS 40.0%
TAGATCAATGCAGCAATGGA+GGG - tig0032655:800-819 Msa1449580:CDS 40.0%
! AGCTATCAGTTTGTGGATTG+TGG - tig0032655:898-917 Msa1449580:CDS 40.0%
! GCTATCAGTTTGTGGATTGT+GGG - tig0032655:899-918 Msa1449580:CDS 40.0%
! AATGATGGAGAACAGAGGAT+TGG + tig0032655:941-960 None:intergenic 40.0%
!! CATCAGACCACTTTTATGGT+TGG + tig0032655:1220-1239 None:intergenic 40.0%
GACATATACCCATGTGCATG+CGG + tig0032655:7-26 None:intergenic 45.0%
! GTCACGATATTGACTAGGCA+TGG + tig0032655:38-57 None:intergenic 45.0%
GAACAATGCGGTAAACAAGC+TGG - tig0032655:235-254 Msa1449580:three_prime_UTR 45.0%
TGGCTGCAACAAAGGTTGTT+TGG + tig0032655:274-293 None:intergenic 45.0%
!! AACCGTATTGGCTGCAACAA+AGG + tig0032655:282-301 None:intergenic 45.0%
TTTGTTGCAGCCAATACGGT+TGG - tig0032655:281-300 Msa1449580:exon 45.0%
GGTACCACAGATGAATACTG+TGG - tig0032655:307-326 Msa1449580:CDS 45.0%
AATTGTCATGGCAGTAGTGG+TGG - tig0032655:346-365 Msa1449580:CDS 45.0%
ATCAACCAGAGCAACATGGA+TGG - tig0032655:410-429 Msa1449580:CDS 45.0%
TCAACCAGAGCAACATGGAT+GGG - tig0032655:411-430 Msa1449580:CDS 45.0%
! TACTTGCTACGCCATGAGTA+AGG + tig0032655:484-503 None:intergenic 45.0%
TACTCATGGCGTAGCAAGTA+TGG - tig0032655:484-503 Msa1449580:intron 45.0%
!! CTTCTTGTGGAAAGTGTCTC+AGG - tig0032655:548-567 Msa1449580:intron 45.0%
GTAGATCAATGCAGCAATGG+AGG - tig0032655:799-818 Msa1449580:CDS 45.0%
! GCATCAATGATGGAGAACAG+AGG + tig0032655:946-965 None:intergenic 45.0%
GACATATACCCATGTGCATG+CGG + tig0032655:7-26 None:intergenic 45.0%
! GTCACGATATTGACTAGGCA+TGG + tig0032655:38-57 None:intergenic 45.0%
GAACAATGCGGTAAACAAGC+TGG - tig0032655:235-254 Msa1449580:three_prime_UTR 45.0%
TGGCTGCAACAAAGGTTGTT+TGG + tig0032655:274-293 None:intergenic 45.0%
!! AACCGTATTGGCTGCAACAA+AGG + tig0032655:282-301 None:intergenic 45.0%
TTTGTTGCAGCCAATACGGT+TGG - tig0032655:281-300 Msa1449580:exon 45.0%
GGTACCACAGATGAATACTG+TGG - tig0032655:307-326 Msa1449580:CDS 45.0%
AATTGTCATGGCAGTAGTGG+TGG - tig0032655:346-365 Msa1449580:CDS 45.0%
ATCAACCAGAGCAACATGGA+TGG - tig0032655:410-429 Msa1449580:CDS 45.0%
TCAACCAGAGCAACATGGAT+GGG - tig0032655:411-430 Msa1449580:CDS 45.0%
! TACTTGCTACGCCATGAGTA+AGG + tig0032655:484-503 None:intergenic 45.0%
TACTCATGGCGTAGCAAGTA+TGG - tig0032655:484-503 Msa1449580:intron 45.0%
!! CTTCTTGTGGAAAGTGTCTC+AGG - tig0032655:548-567 Msa1449580:intron 45.0%
GTAGATCAATGCAGCAATGG+AGG - tig0032655:799-818 Msa1449580:CDS 45.0%
! GCATCAATGATGGAGAACAG+AGG + tig0032655:946-965 None:intergenic 45.0%
ATATACCCATGTGCATGCGG+CGG + tig0032655:4-23 None:intergenic 50.0%
CAATGCGGTAAACAAGCTGG+AGG - tig0032655:238-257 Msa1449580:three_prime_UTR 50.0%
TGGTACCACACCAACCGTAT+TGG + tig0032655:294-313 None:intergenic 50.0%
CGGACCACAGTATTCATCTG+TGG + tig0032655:314-333 None:intergenic 50.0%
AAGTCCCATCCATGTTGCTC+TGG + tig0032655:418-437 None:intergenic 50.0%
CATGGCGTAGCAAGTATGGT+TGG - tig0032655:488-507 Msa1449580:intron 50.0%
!!! ACTGCTTTTTGTGGCCCTGT+TGG - tig0032655:511-530 Msa1449580:intron 50.0%
GCAGGTGACAAATTCAGGGA+CGG - tig0032655:753-772 Msa1449580:CDS 50.0%
CAGGTGACAAATTCAGGGAC+GGG - tig0032655:754-773 Msa1449580:CDS 50.0%
TCAATGCAGCAATGGAGGGT+TGG - tig0032655:804-823 Msa1449580:CDS 50.0%
CAGCAATGGAGGGTTGGATT+TGG - tig0032655:810-829 Msa1449580:CDS 50.0%
GGAGGGTTGGATTTGGATGT+AGG - tig0032655:817-836 Msa1449580:CDS 50.0%
AGAATCGACACAGATGGCAG+AGG - tig0032655:850-869 Msa1449580:CDS 50.0%
AGATGGCAGAGGATACCAAC+AGG - tig0032655:861-880 Msa1449580:CDS 50.0%
GATGGCAGAGGATACCAACA+GGG - tig0032655:862-881 Msa1449580:CDS 50.0%
! TTACTACAGCACTGAGGCAG+AGG + tig0032655:993-1012 None:intergenic 50.0%
ATATACCCATGTGCATGCGG+CGG + tig0032655:4-23 None:intergenic 50.0%
CAATGCGGTAAACAAGCTGG+AGG - tig0032655:238-257 Msa1449580:three_prime_UTR 50.0%
TGGTACCACACCAACCGTAT+TGG + tig0032655:294-313 None:intergenic 50.0%
CGGACCACAGTATTCATCTG+TGG + tig0032655:314-333 None:intergenic 50.0%
AAGTCCCATCCATGTTGCTC+TGG + tig0032655:418-437 None:intergenic 50.0%
CATGGCGTAGCAAGTATGGT+TGG - tig0032655:488-507 Msa1449580:intron 50.0%
!!! ACTGCTTTTTGTGGCCCTGT+TGG - tig0032655:511-530 Msa1449580:intron 50.0%
GCAGGTGACAAATTCAGGGA+CGG - tig0032655:753-772 Msa1449580:CDS 50.0%
CAGGTGACAAATTCAGGGAC+GGG - tig0032655:754-773 Msa1449580:CDS 50.0%
TCAATGCAGCAATGGAGGGT+TGG - tig0032655:804-823 Msa1449580:CDS 50.0%
CAGCAATGGAGGGTTGGATT+TGG - tig0032655:810-829 Msa1449580:CDS 50.0%
GGAGGGTTGGATTTGGATGT+AGG - tig0032655:817-836 Msa1449580:CDS 50.0%
AGAATCGACACAGATGGCAG+AGG - tig0032655:850-869 Msa1449580:CDS 50.0%
AGATGGCAGAGGATACCAAC+AGG - tig0032655:861-880 Msa1449580:CDS 50.0%
GATGGCAGAGGATACCAACA+GGG - tig0032655:862-881 Msa1449580:CDS 50.0%
! TTACTACAGCACTGAGGCAG+AGG + tig0032655:993-1012 None:intergenic 50.0%
! TGCAGCCAATACGGTTGGTG+TGG - tig0032655:286-305 Msa1449580:CDS 55.0%
TGTCATGGCAGTAGTGGTGG+TGG - tig0032655:349-368 Msa1449580:CDS 55.0%
CTTGACCACGAGGTCCAACA+GGG + tig0032655:528-547 None:intergenic 55.0%
CCACAAGAAGCTTGACCACG+AGG + tig0032655:538-557 None:intergenic 55.0%
CCTCGTGGTCAAGCTTCTTG+TGG - tig0032655:535-554 Msa1449580:intron 55.0%
! AAATTCAGGGACGGGAGCAC+AGG - tig0032655:762-781 Msa1449580:CDS 55.0%
CTGAGGCAGAGGAAGCAGAT+TGG + tig0032655:982-1001 None:intergenic 55.0%
! GGTCTGATGTGATGGAGCTC+CGG - tig0032655:1231-1250 Msa1449580:five_prime_UTR 55.0%
! TGATGTGATGGAGCTCCGGT+GGG - tig0032655:1235-1254 Msa1449580:five_prime_UTR 55.0%
! TGCAGCCAATACGGTTGGTG+TGG - tig0032655:286-305 Msa1449580:CDS 55.0%
TGTCATGGCAGTAGTGGTGG+TGG - tig0032655:349-368 Msa1449580:CDS 55.0%
CTTGACCACGAGGTCCAACA+GGG + tig0032655:528-547 None:intergenic 55.0%
CCACAAGAAGCTTGACCACG+AGG + tig0032655:538-557 None:intergenic 55.0%
CCTCGTGGTCAAGCTTCTTG+TGG - tig0032655:535-554 Msa1449580:intron 55.0%
! AAATTCAGGGACGGGAGCAC+AGG - tig0032655:762-781 Msa1449580:CDS 55.0%
CTGAGGCAGAGGAAGCAGAT+TGG + tig0032655:982-1001 None:intergenic 55.0%
! GGTCTGATGTGATGGAGCTC+CGG - tig0032655:1231-1250 Msa1449580:five_prime_UTR 55.0%
GCTTGACCACGAGGTCCAAC+AGG + tig0032655:529-548 None:intergenic 60.0%
! CTGATGTGATGGAGCTCCGG+TGG - tig0032655:1234-1253 Msa1449580:five_prime_UTR 60.0%
GCTTGACCACGAGGTCCAAC+AGG + tig0032655:529-548 None:intergenic 60.0%
! CTGATGTGATGGAGCTCCGG+TGG - tig0032655:1234-1253 Msa1449580:five_prime_UTR 60.0%
TGTGGCCCTGTTGGACCTCG+TGG - tig0032655:520-539 Msa1449580:intron 65.0%
TGTGGCCCTGTTGGACCTCG+TGG - tig0032655:520-539 Msa1449580:intron 65.0%
Chromosome Type Strat End Strand Name
tig0032655 gene 1 1257 1 ID=Msa1449580;Name=Msa1449580
tig0032655 mRNA 1 1257 1 ID=Msa1449580-mRNA-1;Parent=Msa1449580;Name=Msa1449580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=168|1|1|1|1|1|2|282|206
tig0032655 exon 1 501 1 ID=Msa1449580-mRNA-1:exon:17027;Parent=Msa1449580-mRNA-1
tig0032655 exon 688 1257 688 ID=Msa1449580-mRNA-1:exon:17026;Parent=Msa1449580-mRNA-1
tig0032655 five_prime_UTR 1090 1257 1090 ID=Msa1449580-mRNA-1:five_prime_utr;Parent=Msa1449580-mRNA-1
tig0032655 CDS 688 1089 688 ID=Msa1449580-mRNA-1:cds;Parent=Msa1449580-mRNA-1
tig0032655 CDS 283 501 283 ID=Msa1449580-mRNA-1:cds;Parent=Msa1449580-mRNA-1
tig0032655 three_prime_UTR 1 282 1 ID=Msa1449580-mRNA-1:three_prime_utr;Parent=Msa1449580-mRNA-1
Gene Sequence

>Msa1449580

ATGGGAAAATTTGGAGTAAGCCTAGCGGTATTAACATTGGTGTGTTTGACTGCAACAACAGTTGCAGAACAATGCGGTAAACAAGCTGGAGGAAAAACATGTCCAAACAACCTTTGTTGCAGCCAATACGGTTGGTGTGGTACCACAGATGAATACTGTGGTCCGAACTGTCAGAGTAATTGTCATGGCAGTAGTGGTGGTGGTGAAAGTGCTTCTAATGTTCGTGCTACTTATCATTACTATCAACCAGAGCAACATGGATGGGACTTAAACGCTGTAAGTGCTTATTGTTCAACTTGGGATGCAAGCAAACCTTACTCATGGCGTAGCAAGTATGGTTGGACTGCTTTTTGTGGCCCTGTTGGACCTCGTGGTCAAGCTTCTTGTGGAAAGTGTCTCAGGGTGACAAATTCAGGGACGGGAGCACAGGAAACAGTGAGAATTGTAGATCAATGCAGCAATGGAGGGTTGGATTTGGATGTAGGAGTGTTTAACAGAATCGACACAGATGGCAGAGGATACCAACAGGGACATCTTATTGTAAGCTATCAGTTTGTGGATTGTGGGAATGAGCTAGACATGACCAATCCTCTGTTCTCCATCATTGATGCTGAACAATAA
Protein sequence

>Msa1449580

MGKFGVSLAVLTLVCLTATTVAEQCGKQAGGKTCPNNLCCSQYGWCGTTDEYCGPNCQSNCHGSSGGGESASNVRATYHYYQPEQHGWDLNAVSAYCSTWDASKPYSWRSKYGWTAFCGPVGPRGQASCGKCLRVTNSGTGAQETVRIVDQCSNGGLDLDVGVFNRIDTDGRGYQQGHLIVSYQFVDCGNELDMTNPLFSIIDAEQ*