AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376660 MtrunA17_Chr3g0145751 92.754 69 0 2 1 69 1 64 1.06e-35 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTCACAGCTTGACTTTC+TGG 0.027355 tig0019825:-130578 Msa1376660:CDS
TGCATTAGGACTTATATTTA+AGG 0.141077 tig0019825:-130305 Msa1376660:three_prime_UTR
CTTTCTCTTTCTGCTTCTGC+TGG 0.246256 tig0019825:-133199 Msa1376660:five_prime_UTR
GATAGAGCGGTTCCTTTGAT+TGG 0.346136 tig0019825:+133016 None:intergenic
GAATTGCAATATCTTGCATT+AGG 0.362070 tig0019825:-130319 Msa1376660:three_prime_UTR
GTGTCACAGCTTGACTTTCT+GGG 0.362844 tig0019825:-130577 Msa1376660:CDS
TAGGATTGGCACTGTGCATT+TGG 0.363887 tig0019825:+130542 None:intergenic
ATTCTGGTGGGACCTCAGTT+TGG 0.371991 tig0019825:-130782 Msa1376660:CDS
GGAACCGCTCTATCAATAAT+AGG 0.378563 tig0019825:-133007 Msa1376660:CDS
CAAGCTCAGCCACCGGAATC+TGG 0.379086 tig0019825:-133071 Msa1376660:CDS
CGAAGTTACAAAATCACAAA+TGG 0.387457 tig0019825:+130406 None:intergenic
ATAGAGCGGTTCCTTTGATT+GGG 0.419860 tig0019825:+133017 None:intergenic
TTTCTCTTTCTGCTTCTGCT+GGG 0.448802 tig0019825:-133198 Msa1376660:five_prime_UTR
AAGAAGAGACTGAGAGTCAG+AGG 0.454007 tig0019825:-133125 Msa1376660:five_prime_UTR
GTCCCACCAGAATACATAAC+TGG 0.456812 tig0019825:+130792 None:intergenic
GAAAATAAGGACAAAATAGT+TGG 0.469040 tig0019825:+130163 None:intergenic
TCAGTTCAGACTCCCTCATC+AGG 0.470456 tig0019825:-130757 Msa1376660:intron
ACAAATGGGTAGTCAAGTAA+AGG 0.483918 tig0019825:+130421 None:intergenic
TAAATAAAGCTTACCTGATG+AGG 0.484449 tig0019825:+130744 None:intergenic
GAAGAGGAAGCACTCGCAGC+TGG 0.484647 tig0019825:+130601 None:intergenic
CAAATGGGTAGTCAAGTAAA+GGG 0.492979 tig0019825:+130422 None:intergenic
ACCACCACAGGACCAGATTC+CGG 0.497624 tig0019825:+133059 None:intergenic
AATAGGCGGAAATCATCATC+TGG 0.506410 tig0019825:-132990 Msa1376660:intron
ATCCATCATGTGCTGAAACT+AGG 0.524448 tig0019825:+130523 None:intergenic
GAAGTTACAAAATCACAAAT+GGG 0.528232 tig0019825:+130407 None:intergenic
ACAAAATAGTTGGTTCATGT+AGG 0.539542 tig0019825:+130173 None:intergenic
TAGCGCTTCACCACCACCAC+AGG 0.546744 tig0019825:+133047 None:intergenic
TGAGCTTGAGCTTGAGCGTG+AGG 0.552238 tig0019825:+133086 None:intergenic
ATCCTAGTTTCAGCACATGA+TGG 0.552536 tig0019825:-130525 Msa1376660:CDS
TCAAGCTCAAGCTCAGCCAC+CGG 0.553933 tig0019825:-133078 Msa1376660:CDS
AAATAAAGCTTACCTGATGA+GGG 0.555627 tig0019825:+130745 None:intergenic
TCATGTGCTGAAACTAGGAT+TGG 0.568680 tig0019825:+130528 None:intergenic
GTCAAGCTGTGACACAGAAG+AGG 0.569534 tig0019825:+130585 None:intergenic
GGAATCTGGTCCTGTGGTGG+TGG 0.575807 tig0019825:-133057 Msa1376660:CDS
TCCGCCTATTATTGATAGAG+CGG 0.578688 tig0019825:+133003 None:intergenic
CTTGATCGATCAAACAGTGT+TGG 0.579556 tig0019825:-132164 Msa1376660:CDS
TAGTTTCAGCACATGATGGA+TGG 0.583477 tig0019825:-130521 Msa1376660:CDS
GCTATGCTCCGCCCAATCAA+AGG 0.584395 tig0019825:-133028 Msa1376660:CDS
GAGCGGTTCCTTTGATTGGG+CGG 0.584544 tig0019825:+133020 None:intergenic
GCCACCGGAATCTGGTCCTG+TGG 0.595031 tig0019825:-133063 Msa1376660:CDS
AAACCAGTTATGTATTCTGG+TGG 0.598244 tig0019825:-130795 Msa1376660:CDS
GAGCTTGAGCTTGAGCGTGA+GGG 0.601515 tig0019825:+133087 None:intergenic
TCAGCACATGATGGATGGCA+TGG 0.602398 tig0019825:-130516 Msa1376660:CDS
ACCGGAATCTGGTCCTGTGG+TGG 0.616133 tig0019825:-133060 Msa1376660:CDS
ATTGCAATTCACACCTACAC+AGG 0.627927 tig0019825:+130332 None:intergenic
AGAAGAGACTGAGAGTCAGA+GGG 0.632966 tig0019825:-133124 Msa1376660:five_prime_UTR
AACCAGTTATGTATTCTGGT+GGG 0.640003 tig0019825:-130794 Msa1376660:CDS
AATATGTGTGACGCCTGTGT+AGG 0.649218 tig0019825:-130345 Msa1376660:three_prime_UTR
ACCACAGGACCAGATTCCGG+TGG 0.684814 tig0019825:+133062 None:intergenic
ACCGCTCTATCAATAATAGG+CGG 0.687958 tig0019825:-133004 Msa1376660:CDS
TCTGAACTGAGTCCAAACTG+AGG 0.739116 tig0019825:+130770 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAATAAATAAGTCAT+CGG + tig0019825:130559-130578 None:intergenic 10.0%
!! TTACAAACAAAAAAAAAAAA+AGG + tig0019825:131062-131081 None:intergenic 10.0%
!! ACAAAAAACAAAAAAAAAAA+AGG + tig0019825:132057-132076 None:intergenic 10.0%
!! AGTAAAGAATTAAAGAAAAA+AGG + tig0019825:130450-130469 None:intergenic 15.0%
!!! TTACATTGATTGAAGTAAAA+AGG + tig0019825:130703-130722 None:intergenic 20.0%
!! TAATGTATCATCCTAAATAA+TGG - tig0019825:130720-130739 Msa1376660:intron 20.0%
!! TTATGTGCCTATAATTTATA+GGG + tig0019825:130765-130784 None:intergenic 20.0%
!! TTCTATCAAATTATGTAGAT+CGG - tig0019825:131199-131218 Msa1376660:intron 20.0%
!!! TTTTTTGTTTTTTGTGTGTT+TGG - tig0019825:132062-132081 Msa1376660:intron 20.0%
!!! TTACTTTTTAATCATCTTTG+TGG - tig0019825:132364-132383 Msa1376660:intron 20.0%
!! ATAAATAATATACGCTCAAA+TGG + tig0019825:132438-132457 None:intergenic 20.0%
!! TTGAGCGTATATTATTTATA+CGG - tig0019825:132439-132458 Msa1376660:intron 20.0%
!! AAGATCATGTTAGATTTAAA+AGG + tig0019825:132734-132753 None:intergenic 20.0%
!!! GTTTTGATAATTTAAATGCA+TGG - tig0019825:133141-133160 Msa1376660:five_prime_UTR 20.0%
! TGAGATTTGAATTAGTTAGT+TGG + tig0019825:130636-130655 None:intergenic 25.0%
! AGATAGAATTAGAGTTAGTT+GGG + tig0019825:130663-130682 None:intergenic 25.0%
! AAGATAGAATTAGAGTTAGT+TGG + tig0019825:130664-130683 None:intergenic 25.0%
!! TATCATCCTAAATAATGGAA+CGG - tig0019825:130725-130744 Msa1376660:intron 25.0%
! CTTATGTGCCTATAATTTAT+AGG + tig0019825:130766-130785 None:intergenic 25.0%
!!! GAACTATTAGTTTGTATTGT+TGG - tig0019825:130799-130818 Msa1376660:CDS 25.0%
! CAATGAATCTTCTTGAATAA+TGG + tig0019825:131341-131360 None:intergenic 25.0%
!! TGTCTAATATTTAGTGTTGA+TGG - tig0019825:131927-131946 Msa1376660:intron 25.0%
!!! GAATCATTTCATAGTTTTAC+TGG + tig0019825:132008-132027 None:intergenic 25.0%
! TAGTCAATTAAAGAAAGAAC+TGG + tig0019825:132091-132110 None:intergenic 25.0%
! TTGACTAATTACTATAAGCT+TGG - tig0019825:132104-132123 Msa1376660:intron 25.0%
!!! AAGGAACAACATATTTTAGT+TGG + tig0019825:132206-132225 None:intergenic 25.0%
! AAACACTAATGAACTGTATA+AGG + tig0019825:132225-132244 None:intergenic 25.0%
! TATAGTTTCTAAATTGCTGT+TGG - tig0019825:132398-132417 Msa1376660:intron 25.0%
!! ATAGTTTCTAAATTGCTGTT+GGG - tig0019825:132399-132418 Msa1376660:intron 25.0%
! GAAGTTACAAAATCACAAAT+GGG + tig0019825:132982-133001 None:intergenic 25.0%
!! CATTTGTGATTTTGTAACTT+CGG - tig0019825:132981-133000 Msa1376660:intron 25.0%
! TGCATTAGGACTTATATTTA+AGG - tig0019825:133081-133100 Msa1376660:CDS 25.0%
TAATAAATAAGTCATCGGAC+AGG + tig0019825:130554-130573 None:intergenic 30.0%
GATTTGAATTAGTTAGTTGG+AGG + tig0019825:130633-130652 None:intergenic 30.0%
! AGTTTGTATTGTTGGATTAG+TGG - tig0019825:130807-130826 Msa1376660:intron 30.0%
ACATATTGGTATTCAACTCA+AGG + tig0019825:130879-130898 None:intergenic 30.0%
! TGAGTTGAATACCAATATGT+TGG - tig0019825:130879-130898 Msa1376660:intron 30.0%
! GAACTTTTACATAGATCTGA+TGG + tig0019825:130987-131006 None:intergenic 30.0%
AGAAACAAACAAAATAGAGC+AGG + tig0019825:131158-131177 None:intergenic 30.0%
! AACATTCAAGTTACAGATCA+CGG - tig0019825:131282-131301 Msa1376660:intron 30.0%
! AGAAGATTCATTGCTTTAGA+AGG - tig0019825:131348-131367 Msa1376660:intron 30.0%
TACAAGTTCTCACAAACAAA+AGG + tig0019825:131426-131445 None:intergenic 30.0%
TTGTGAGAACTTGTATATGT+TGG - tig0019825:131431-131450 Msa1376660:intron 30.0%
AAGAACTACAGAAATCATCA+GGG + tig0019825:131486-131505 None:intergenic 30.0%
AAAGAACTACAGAAATCATC+AGG + tig0019825:131487-131506 None:intergenic 30.0%
GATGATTTCTGTAGTTCTTT+AGG - tig0019825:131487-131506 Msa1376660:intron 30.0%
!! TACCTTCAATTAGTTTGCAT+AGG - tig0019825:131556-131575 Msa1376660:intron 30.0%
ATCCTATGCAAACTAATTGA+AGG + tig0019825:131561-131580 None:intergenic 30.0%
! GATTGAGATATGCAGATTTT+AGG - tig0019825:131578-131597 Msa1376660:intron 30.0%
!!! ATATGCAGATTTTAGGACTT+AGG - tig0019825:131585-131604 Msa1376660:intron 30.0%
GGAACAATTAACGAAACAAA+AGG + tig0019825:131666-131685 None:intergenic 30.0%
CAGTTAAAATACGAAAAGAC+AGG + tig0019825:131687-131706 None:intergenic 30.0%
CAACAGGTAATGTTAATCAA+TGG + tig0019825:131784-131803 None:intergenic 30.0%
CAAATTTCATAGCATGGAAA+AGG + tig0019825:131903-131922 None:intergenic 30.0%
ATACAGTTCATTAGTGTTTG+AGG - tig0019825:132226-132245 Msa1376660:intron 30.0%
TGAACTACATCAAAACACTT+TGG + tig0019825:132319-132338 None:intergenic 30.0%
TACTATCACCACATTTAAGA+AGG + tig0019825:132488-132507 None:intergenic 30.0%
TATGTGCTACATATACTCTT+AGG - tig0019825:132511-132530 Msa1376660:intron 30.0%
!!! GTTTTTCTGAAATTCGAAAG+AGG + tig0019825:132575-132594 None:intergenic 30.0%
GAAAAACCAGTTATGTATTC+TGG - tig0019825:132588-132607 Msa1376660:intron 30.0%
AAATAAAGCTTACCTGATGA+GGG + tig0019825:132644-132663 None:intergenic 30.0%
TAAATAAAGCTTACCTGATG+AGG + tig0019825:132645-132664 None:intergenic 30.0%
GTTAGATTTAAAAGGCAGAA+GGG + tig0019825:132726-132745 None:intergenic 30.0%
TGTTAGATTTAAAAGGCAGA+AGG + tig0019825:132727-132746 None:intergenic 30.0%
CGAAGTTACAAAATCACAAA+TGG + tig0019825:132983-133002 None:intergenic 30.0%
GAATTGCAATATCTTGCATT+AGG - tig0019825:133067-133086 Msa1376660:CDS 30.0%
! ACAAAATAGTTGGTTCATGT+AGG + tig0019825:133216-133235 None:intergenic 30.0%
AGTTGGAGGAAAGCAAATAA+GGG + tig0019825:130619-130638 None:intergenic 35.0%
TAGTTGGAGGAAAGCAAATA+AGG + tig0019825:130620-130639 None:intergenic 35.0%
! CTAAATAATGGAACGGATCT+GGG - tig0019825:130732-130751 Msa1376660:intron 35.0%
ACTGGGACCCTATAAATTAT+AGG - tig0019825:130755-130774 Msa1376660:intron 35.0%
TTAACTCACGATAACAGTGT+TGG - tig0019825:130846-130865 Msa1376660:intron 35.0%
CTGCTTATTGCATTATAGTG+AGG - tig0019825:131704-131723 Msa1376660:intron 35.0%
TATTGCATTATAGTGAGGAG+TGG - tig0019825:131709-131728 Msa1376660:intron 35.0%
ATTGCATTATAGTGAGGAGT+GGG - tig0019825:131710-131729 Msa1376660:intron 35.0%
!! TGAATCTTTGGGATTGTTGT+AGG + tig0019825:131849-131868 None:intergenic 35.0%
AACAATCCCAAAGATTCATC+TGG - tig0019825:131851-131870 Msa1376660:intron 35.0%
CACTAACCAGATGAATCTTT+GGG + tig0019825:131860-131879 None:intergenic 35.0%
CAGTGACAAATTTCATAGCA+TGG + tig0019825:131909-131928 None:intergenic 35.0%
!! GTTTTCAATCCATTGAGTTG+AGG - tig0019825:131979-131998 Msa1376660:intron 35.0%
ATTGCGATACTTACGACTTA+CGG - tig0019825:132161-132180 Msa1376660:CDS 35.0%
TATCACCACATTTAAGAAGG+CGG + tig0019825:132485-132504 None:intergenic 35.0%
!! TTCTGAAATTCGAAAGAGGT+AGG + tig0019825:132571-132590 None:intergenic 35.0%
AAACCAGTTATGTATTCTGG+TGG - tig0019825:132591-132610 Msa1376660:intron 35.0%
AACCAGTTATGTATTCTGGT+GGG - tig0019825:132592-132611 Msa1376660:intron 35.0%
CAAATGGGTAGTCAAGTAAA+GGG + tig0019825:132967-132986 None:intergenic 35.0%
ACAAATGGGTAGTCAAGTAA+AGG + tig0019825:132968-132987 None:intergenic 35.0%
TTTCTCTTTCTGCTTCTGCT+GGG - tig0019825:130188-130207 Msa1376660:three_prime_UTR 40.0%
! TCTCTCTCTCTCTCTAAGAA+AGG - tig0019825:130238-130257 Msa1376660:three_prime_UTR 40.0%
ATAGAGCGGTTCCTTTGATT+GGG + tig0019825:130372-130391 None:intergenic 40.0%
GGAACCGCTCTATCAATAAT+AGG - tig0019825:130379-130398 Msa1376660:three_prime_UTR 40.0%
ACCGCTCTATCAATAATAGG+CGG - tig0019825:130382-130401 Msa1376660:three_prime_UTR 40.0%
TCCGCCTATTATTGATAGAG+CGG + tig0019825:130386-130405 None:intergenic 40.0%
AATAGGCGGAAATCATCATC+TGG - tig0019825:130396-130415 Msa1376660:three_prime_UTR 40.0%
CCAGATCCGTTCCATTATTT+AGG + tig0019825:130734-130753 None:intergenic 40.0%
! CCTAAATAATGGAACGGATC+TGG - tig0019825:130731-130750 Msa1376660:intron 40.0%
TCGTGAGTTAATATGTGCCT+TGG + tig0019825:130837-130856 None:intergenic 40.0%
GAAGGCTAAAGCCAACATAT+TGG + tig0019825:130893-130912 None:intergenic 40.0%
ATTTATCTCCCATGAGACCA+TGG + tig0019825:130922-130941 None:intergenic 40.0%
GCATAATTCACAGTGAGCAA+TGG - tig0019825:131103-131122 Msa1376660:intron 40.0%
CTTGATCGATCAAACAGTGT+TGG - tig0019825:131222-131241 Msa1376660:intron 40.0%
GTTCTCACAAACAAAAGGCA+GGG + tig0019825:131421-131440 None:intergenic 40.0%
AGTTCTCACAAACAAAAGGC+AGG + tig0019825:131422-131441 None:intergenic 40.0%
TTGCATTATAGTGAGGAGTG+GGG - tig0019825:131711-131730 Msa1376660:intron 40.0%
TTAACATTACCTGTTGCCCA+TGG - tig0019825:131788-131807 Msa1376660:intron 40.0%
CCACTAACCAGATGAATCTT+TGG + tig0019825:131861-131880 None:intergenic 40.0%
CCAAAGATTCATCTGGTTAG+TGG - tig0019825:131858-131877 Msa1376660:intron 40.0%
AAGGTCAATTACTGCAACAG+TGG + tig0019825:132038-132057 None:intergenic 40.0%
TTGCGATACTTACGACTTAC+GGG - tig0019825:132162-132181 Msa1376660:CDS 40.0%
TTGATGTAGTTCAAGCTGAG+AGG - tig0019825:132326-132345 Msa1376660:intron 40.0%
AATAGCCGCCTTCTTAAATG+TGG - tig0019825:132477-132496 Msa1376660:intron 40.0%
ATTTAAAAGGCAGAAGGGAG+GGG + tig0019825:132721-132740 None:intergenic 40.0%
GATTTAAAAGGCAGAAGGGA+GGG + tig0019825:132722-132741 None:intergenic 40.0%
AGATTTAAAAGGCAGAAGGG+AGG + tig0019825:132723-132742 None:intergenic 40.0%
TCATGTGCTGAAACTAGGAT+TGG + tig0019825:132861-132880 None:intergenic 40.0%
ATCCTAGTTTCAGCACATGA+TGG - tig0019825:132861-132880 Msa1376660:intron 40.0%
ATCCATCATGTGCTGAAACT+AGG + tig0019825:132866-132885 None:intergenic 40.0%
! TAGTTTCAGCACATGATGGA+TGG - tig0019825:132865-132884 Msa1376660:intron 40.0%
ATTGCAATTCACACCTACAC+AGG + tig0019825:133057-133076 None:intergenic 40.0%
CTTTCTCTTTCTGCTTCTGC+TGG - tig0019825:130187-130206 Msa1376660:three_prime_UTR 45.0%
AAGAAGAGACTGAGAGTCAG+AGG - tig0019825:130261-130280 Msa1376660:three_prime_UTR 45.0%
AGAAGAGACTGAGAGTCAGA+GGG - tig0019825:130262-130281 Msa1376660:three_prime_UTR 45.0%
GATAGAGCGGTTCCTTTGAT+TGG + tig0019825:130373-130392 None:intergenic 45.0%
AATAAGTCATCGGACAGGAC+AGG + tig0019825:130549-130568 None:intergenic 45.0%
!! GTTGGATTAGTGGATGTCCA+AGG - tig0019825:130817-130836 Msa1376660:intron 45.0%
! CTTTAGCCTTCTATCTGCCA+TGG - tig0019825:130902-130921 Msa1376660:intron 45.0%
ATGAGACCATGGCAGATAGA+AGG + tig0019825:130911-130930 None:intergenic 45.0%
TTCTATCTGCCATGGTCTCA+TGG - tig0019825:130910-130929 Msa1376660:intron 45.0%
TCTATCTGCCATGGTCTCAT+GGG - tig0019825:130911-130930 Msa1376660:intron 45.0%
CTTGTAGCTCTTGATGCAAG+TGG - tig0019825:131026-131045 Msa1376660:intron 45.0%
! GTGAGCAATGGCAAAGCTTT+TGG - tig0019825:131115-131134 Msa1376660:intron 45.0%
AGCAGGAACTGACAGCAAAT+GGG + tig0019825:131141-131160 None:intergenic 45.0%
CTCACGATCACTAAGAAGCT+GGG + tig0019825:131394-131413 None:intergenic 45.0%
ACTCACGATCACTAAGAAGC+TGG + tig0019825:131395-131414 None:intergenic 45.0%
TGCATTATAGTGAGGAGTGG+GGG - tig0019825:131712-131731 Msa1376660:intron 45.0%
ACCTGGAAACAGAAGTCCAT+GGG + tig0019825:131807-131826 None:intergenic 45.0%
AACCTGGAAACAGAAGTCCA+TGG + tig0019825:131808-131827 None:intergenic 45.0%
ATGGACTTCTGTTTCCAGGT+TGG - tig0019825:131807-131826 Msa1376660:intron 45.0%
TGTATTACAGCTGTCCAACC+TGG + tig0019825:131824-131843 None:intergenic 45.0%
TACTGGTGACCTCAACTCAA+TGG + tig0019825:131991-132010 None:intergenic 45.0%
ACTTACGACTTACGGGAGTT+TGG - tig0019825:132169-132188 Msa1376660:CDS 45.0%
GTCCCACCAGAATACATAAC+TGG + tig0019825:132597-132616 None:intergenic 45.0%
TCTGAACTGAGTCCAAACTG+AGG + tig0019825:132619-132638 None:intergenic 45.0%
TGTGTCACAGCTTGACTTTC+TGG - tig0019825:132808-132827 Msa1376660:intron 45.0%
GTGTCACAGCTTGACTTTCT+GGG - tig0019825:132809-132828 Msa1376660:intron 45.0%
! TAGGATTGGCACTGTGCATT+TGG + tig0019825:132847-132866 None:intergenic 45.0%
AATATGTGTGACGCCTGTGT+AGG - tig0019825:133041-133060 Msa1376660:CDS 45.0%
! TAATGGAACGGATCTGGGAC+TGG - tig0019825:130737-130756 Msa1376660:intron 50.0%
! AATGGAACGGATCTGGGACT+GGG - tig0019825:130738-130757 Msa1376660:intron 50.0%
GAGCAGGAACTGACAGCAAA+TGG + tig0019825:131142-131161 None:intergenic 50.0%
AACAGAAGTCCATGGGCAAC+AGG + tig0019825:131800-131819 None:intergenic 50.0%
ATTCTGGTGGGACCTCAGTT+TGG - tig0019825:132604-132623 Msa1376660:intron 50.0%
TCAGTTCAGACTCCCTCATC+AGG - tig0019825:132629-132648 Msa1376660:intron 50.0%
CAGCTGGTGACATAATCTGG+GGG + tig0019825:132772-132791 None:intergenic 50.0%
GCAGCTGGTGACATAATCTG+GGG + tig0019825:132773-132792 None:intergenic 50.0%
CGCAGCTGGTGACATAATCT+GGG + tig0019825:132774-132793 None:intergenic 50.0%
TCGCAGCTGGTGACATAATC+TGG + tig0019825:132775-132794 None:intergenic 50.0%
GTCAAGCTGTGACACAGAAG+AGG + tig0019825:132804-132823 None:intergenic 50.0%
!! TCAGCACATGATGGATGGCA+TGG - tig0019825:132870-132889 Msa1376660:intron 50.0%
GAGCTTGAGCTTGAGCGTGA+GGG + tig0019825:130302-130321 None:intergenic 55.0%
TGAGCTTGAGCTTGAGCGTG+AGG + tig0019825:130303-130322 None:intergenic 55.0%
TCAAGCTCAAGCTCAGCCAC+CGG - tig0019825:130308-130327 Msa1376660:three_prime_UTR 55.0%
ACCACCACAGGACCAGATTC+CGG + tig0019825:130330-130349 None:intergenic 55.0%
GCTATGCTCCGCCCAATCAA+AGG - tig0019825:130358-130377 Msa1376660:three_prime_UTR 55.0%
GAGCGGTTCCTTTGATTGGG+CGG + tig0019825:130369-130388 None:intergenic 55.0%
GCCCATGGACTTCTGTTTCC+AGG - tig0019825:131803-131822 Msa1376660:intron 55.0%
AAAAGGCAGAAGGGAGGGGA+AGG + tig0019825:132717-132736 None:intergenic 55.0%
CAAGCTCAGCCACCGGAATC+TGG - tig0019825:130315-130334 Msa1376660:three_prime_UTR 60.0%
ACCACAGGACCAGATTCCGG+TGG + tig0019825:130327-130346 None:intergenic 60.0%
ACCGGAATCTGGTCCTGTGG+TGG - tig0019825:130326-130345 Msa1376660:three_prime_UTR 60.0%
GGAATCTGGTCCTGTGGTGG+TGG - tig0019825:130329-130348 Msa1376660:three_prime_UTR 60.0%
TAGCGCTTCACCACCACCAC+AGG + tig0019825:130342-130361 None:intergenic 60.0%
!! GAAGAGGAAGCACTCGCAGC+TGG + tig0019825:132788-132807 None:intergenic 60.0%
GCCACCGGAATCTGGTCCTG+TGG - tig0019825:130323-130342 Msa1376660:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0019825 gene 130168 133240 130168 ID=Msa1376660;Name=Msa1376660
tig0019825 mRNA 130168 133240 130168 ID=Msa1376660-mRNA-1;Parent=Msa1376660;Name=Msa1376660-mRNA-1;_AED=0.09;_eAED=0.09;_QI=117|0.66|0.75|1|0.66|1|4|251|160
tig0019825 exon 130168 130632 130168 ID=Msa1376660-mRNA-1:exon:5270;Parent=Msa1376660-mRNA-1
tig0019825 exon 130758 130819 130758 ID=Msa1376660-mRNA-1:exon:5269;Parent=Msa1376660-mRNA-1
tig0019825 exon 132118 132191 132118 ID=Msa1376660-mRNA-1:exon:5268;Parent=Msa1376660-mRNA-1
tig0019825 exon 132991 133240 132991 ID=Msa1376660-mRNA-1:exon:5267;Parent=Msa1376660-mRNA-1
tig0019825 five_prime_UTR 133124 133240 133124 ID=Msa1376660-mRNA-1:five_prime_utr;Parent=Msa1376660-mRNA-1
tig0019825 CDS 132991 133123 132991 ID=Msa1376660-mRNA-1:cds;Parent=Msa1376660-mRNA-1
tig0019825 CDS 132118 132191 132118 ID=Msa1376660-mRNA-1:cds;Parent=Msa1376660-mRNA-1
tig0019825 CDS 130758 130819 130758 ID=Msa1376660-mRNA-1:cds;Parent=Msa1376660-mRNA-1
tig0019825 CDS 130419 130632 130419 ID=Msa1376660-mRNA-1:cds;Parent=Msa1376660-mRNA-1
tig0019825 three_prime_UTR 130168 130418 130168 ID=Msa1376660-mRNA-1:three_prime_utr;Parent=Msa1376660-mRNA-1
Gene Sequence

>Msa1376660

ATGATGCTAGAAAACCCTCACGCTCAAGCTCAAGCTCAGCCACCGGAATCTGGTCCTGTGGTGGTGGTGAAGCGCTATGCTCCGCCCAATCAAAGGAACCGCTCTATCAATAATAGGCGGAAATCATCATCTGATCGGCTTGATCGATCAAACAGTGTTGGCAGTGATATAGAAAAGAATCAAGTTGCTTCTTCAAAAAACATTCAAAAAAACCAGTTATGTATTCTGGTGGGACCTCAGTTTGGACTCAGTTCAGACTCCCTCATCAGATTATGTCACCAGCTGCGAGTGCTTCCTCTTCTGTGTCACAGCTTGACTTTCTGGGAGAACTTCGTCGCCAAATGCACAGTGCCAATCCTAGTTTCAGCACATGATGGATGGCATGGACTTATAATCAAGCTCTTGTTTGTTTGTGATTTTATAATCAAGTTCTTGTCACAGCTGAATGCTGTAAGAACCCTTTACTTGACTACCCATTTGTGA
Protein sequence

>Msa1376660

MMLENPHAQAQAQPPESGPVVVVKRYAPPNQRNRSINNRRKSSSDRLDRSNSVGSDIEKNQVASSKNIQKNQLCILVGPQFGLSSDSLIRLCHQLRVLPLLCHSLTFWENFVAKCTVPILVSAHDGWHGLIIKLLFVCDFIIKFLSQLNAVRTLYLTTHL*