Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1032480 | RHN47559.1 | 88.889 | 90 | 10 | 0 | 1 | 90 | 1 | 90 | 2.49e-50 | 164 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1032480 | A0A396H2K1 | 88.889 | 90 | 10 | 0 | 1 | 90 | 1 | 90 | 1.19e-50 | 164 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006480 | Msa1032480 | 0.801996 | 6.918290e-49 | -8.615850e-47 |
| Msa0712860 | Msa1032480 | 0.818136 | 2.294639e-52 | -8.615850e-47 |
| Msa0744640 | Msa1032480 | 0.818734 | 1.679611e-52 | -8.615850e-47 |
| Msa0800480 | Msa1032480 | 0.821519 | 3.867474e-53 | -8.615850e-47 |
| Msa0841730 | Msa1032480 | 0.817960 | 2.514435e-52 | -8.615850e-47 |
| Msa0148960 | Msa1032480 | 0.849870 | 2.449115e-60 | -8.615850e-47 |
| Msa1032480 | Msa1069340 | 0.835453 | 1.663317e-56 | -8.615850e-47 |
| Msa1032480 | Msa1159170 | 0.815404 | 9.406497e-52 | -8.615850e-47 |
| Msa1032480 | Msa1352000 | 0.804433 | 2.164881e-49 | -8.615850e-47 |
| Msa1032480 | Msa1404050 | 0.808760 | 2.644018e-50 | -8.615850e-47 |
| Msa0293720 | Msa1032480 | 0.823047 | 1.709438e-53 | -8.615850e-47 |
| Msa0870510 | Msa1032480 | 0.811222 | 7.800632e-51 | -8.615850e-47 |
| Msa0905570 | Msa1032480 | 0.814638 | 1.391031e-51 | -8.615850e-47 |
| Msa0974710 | Msa1032480 | 0.800686 | 1.282929e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1032480 | MtrunA17_Chr7g0254281 | 88.889 | 90 | 10 | 0 | 1 | 90 | 1 | 90 | 2.29e-54 | 164 |
| Msa1032480 | MtrunA17_Chr8g0362511 | 43.836 | 73 | 41 | 0 | 15 | 87 | 291 | 363 | 2.88e-18 | 77.8 |
| Msa1032480 | MtrunA17_Chr5g0402821 | 41.429 | 70 | 41 | 0 | 18 | 87 | 253 | 322 | 8.45e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTAAGGACAAACGTCTTT+TGG | 0.272617 | 7_1:+67420829 | Msa1032480:CDS |
| TGAAATCTTGTTAACTCTTA+AGG | 0.281042 | 7_1:+67420814 | Msa1032480:CDS |
| GTTGAAGTCCTTGCTTGTTT+TGG | 0.285125 | 7_1:-67419958 | None:intergenic |
| GAAATATTGCTTTGATGATT+TGG | 0.310586 | 7_1:+67420922 | Msa1032480:CDS |
| GAGCAGTGAACAATTGGTTT+TGG | 0.351180 | 7_1:-67419916 | None:intergenic |
| AACAAGATTTCAATGCAATT+CGG | 0.397605 | 7_1:-67420803 | None:intergenic |
| CTGGTGGAGCAGTGAACAAT+TGG | 0.421197 | 7_1:-67419922 | None:intergenic |
| GCTTTAGAATTGATTAAAGA+TGG | 0.453577 | 7_1:+67420872 | Msa1032480:CDS |
| ATTCACACACACATTAGAAC+AGG | 0.490770 | 7_1:-67421028 | None:intergenic |
| GGAGACACATCTTATATCAA+TGG | 0.499972 | 7_1:+67420767 | Msa1032480:CDS |
| TGGTGTTGTAATGTCTCTGG+TGG | 0.520732 | 7_1:-67419938 | None:intergenic |
| GCTTTGATGATTTGGCGACT+TGG | 0.547204 | 7_1:+67420930 | Msa1032480:CDS |
| CACACACACATTAGAACAGG+AGG | 0.564026 | 7_1:-67421025 | None:intergenic |
| ATGGACTTACATCAGACATG+AGG | 0.617157 | 7_1:-67419990 | None:intergenic |
| TGGACTTACATCAGACATGA+GGG | 0.638298 | 7_1:-67419989 | None:intergenic |
| TTACAACACCAAAACAAGCA+AGG | 0.668619 | 7_1:+67419950 | Msa1032480:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGAAAAAATAGTGTAA+AGG | - | chr7_1:67420407-67420426 | None:intergenic | 15.0% |
| !!! | TTAAGATTTTTCTTTTCATT+CGG | + | chr7_1:67420994-67421013 | Msa1032480:exon | 15.0% |
| !! | AAAAAGAAAAAATAGTGTAA+AGG | - | chr7_1:67420407-67420426 | None:intergenic | 15.0% |
| !!! | TTAAGATTTTTCTTTTCATT+CGG | + | chr7_1:67420994-67421013 | Msa1032480:exon | 15.0% |
| !! | TAATAACTTAAAAAGAGAGA+TGG | - | chr7_1:67420012-67420031 | None:intergenic | 20.0% |
| !!! | TGATTTTAGATGTTTCAATT+TGG | + | chr7_1:67420109-67420128 | Msa1032480:intron | 20.0% |
| !! | TTTAATATCAACTGAATGTT+TGG | + | chr7_1:67420141-67420160 | Msa1032480:intron | 20.0% |
| !! | TTAATATCAACTGAATGTTT+GGG | + | chr7_1:67420142-67420161 | Msa1032480:intron | 20.0% |
| !! | ATCTGATCAAAAAAATTACA+TGG | + | chr7_1:67420296-67420315 | Msa1032480:intron | 20.0% |
| !! | ATAGTTGAAAAAGATGATAA+TGG | + | chr7_1:67420746-67420765 | Msa1032480:CDS | 20.0% |
| !!! | GATAATCTTCAATTTTAAGA+AGG | + | chr7_1:67420952-67420971 | Msa1032480:CDS | 20.0% |
| !! | TAATAACTTAAAAAGAGAGA+TGG | - | chr7_1:67420012-67420031 | None:intergenic | 20.0% |
| !!! | TGATTTTAGATGTTTCAATT+TGG | + | chr7_1:67420109-67420128 | Msa1032480:intron | 20.0% |
| !! | TTTAATATCAACTGAATGTT+TGG | + | chr7_1:67420141-67420160 | Msa1032480:intron | 20.0% |
| !! | TTAATATCAACTGAATGTTT+GGG | + | chr7_1:67420142-67420161 | Msa1032480:intron | 20.0% |
| !! | ATCTGATCAAAAAAATTACA+TGG | + | chr7_1:67420296-67420315 | Msa1032480:intron | 20.0% |
| !! | ATAGTTGAAAAAGATGATAA+TGG | + | chr7_1:67420746-67420765 | Msa1032480:CDS | 20.0% |
| !!! | GATAATCTTCAATTTTAAGA+AGG | + | chr7_1:67420952-67420971 | Msa1032480:CDS | 20.0% |
| ! | TAATATCAACTGAATGTTTG+GGG | + | chr7_1:67420143-67420162 | Msa1032480:intron | 25.0% |
| ! | GATTTCAACCAAATCTAAAT+TGG | + | chr7_1:67420196-67420215 | Msa1032480:intron | 25.0% |
| ! | AATGCAAACCAATTTAGATT+TGG | - | chr7_1:67420207-67420226 | None:intergenic | 25.0% |
| ! | AAAAAATTACATGGTTGACT+TGG | + | chr7_1:67420305-67420324 | Msa1032480:intron | 25.0% |
| ! | AAAGTCAACACTTATATCAT+TGG | - | chr7_1:67420382-67420401 | None:intergenic | 25.0% |
| !! | GATACTTTTATGAGTATGTT+TGG | + | chr7_1:67420435-67420454 | Msa1032480:intron | 25.0% |
| ! | GACTTTCGTAAAAAATTACA+TGG | + | chr7_1:67420497-67420516 | Msa1032480:intron | 25.0% |
| ! | TTCCATTTACATGTATTGTT+CGG | + | chr7_1:67420574-67420593 | Msa1032480:intron | 25.0% |
| !!! | TTCGTTGTGTTTTTTACATT+AGG | + | chr7_1:67420694-67420713 | Msa1032480:intron | 25.0% |
| !!! | TTTTTTACATTAGGTGAAAG+AGG | + | chr7_1:67420703-67420722 | Msa1032480:intron | 25.0% |
| ! | AACAAGATTTCAATGCAATT+CGG | - | chr7_1:67420806-67420825 | None:intergenic | 25.0% |
| ! | TGAAATCTTGTTAACTCTTA+AGG | + | chr7_1:67420814-67420833 | Msa1032480:CDS | 25.0% |
| !!! | GCTTTAGAATTGATTAAAGA+TGG | + | chr7_1:67420872-67420891 | Msa1032480:CDS | 25.0% |
| !! | GAAATATTGCTTTGATGATT+TGG | + | chr7_1:67420922-67420941 | Msa1032480:CDS | 25.0% |
| ! | TAATATCAACTGAATGTTTG+GGG | + | chr7_1:67420143-67420162 | Msa1032480:intron | 25.0% |
| ! | GATTTCAACCAAATCTAAAT+TGG | + | chr7_1:67420196-67420215 | Msa1032480:intron | 25.0% |
| ! | AATGCAAACCAATTTAGATT+TGG | - | chr7_1:67420207-67420226 | None:intergenic | 25.0% |
| ! | AAAAAATTACATGGTTGACT+TGG | + | chr7_1:67420305-67420324 | Msa1032480:intron | 25.0% |
| ! | AAAGTCAACACTTATATCAT+TGG | - | chr7_1:67420382-67420401 | None:intergenic | 25.0% |
| !! | GATACTTTTATGAGTATGTT+TGG | + | chr7_1:67420435-67420454 | Msa1032480:intron | 25.0% |
| ! | GACTTTCGTAAAAAATTACA+TGG | + | chr7_1:67420497-67420516 | Msa1032480:intron | 25.0% |
| ! | TTCCATTTACATGTATTGTT+CGG | + | chr7_1:67420574-67420593 | Msa1032480:intron | 25.0% |
| !!! | TTCGTTGTGTTTTTTACATT+AGG | + | chr7_1:67420694-67420713 | Msa1032480:intron | 25.0% |
| !!! | TTTTTTACATTAGGTGAAAG+AGG | + | chr7_1:67420703-67420722 | Msa1032480:intron | 25.0% |
| ! | AACAAGATTTCAATGCAATT+CGG | - | chr7_1:67420806-67420825 | None:intergenic | 25.0% |
| ! | TGAAATCTTGTTAACTCTTA+AGG | + | chr7_1:67420814-67420833 | Msa1032480:CDS | 25.0% |
| !!! | GCTTTAGAATTGATTAAAGA+TGG | + | chr7_1:67420872-67420891 | Msa1032480:CDS | 25.0% |
| !! | GAAATATTGCTTTGATGATT+TGG | + | chr7_1:67420922-67420941 | Msa1032480:CDS | 25.0% |
| GTAGTAAATGAAGTAGAGAT+AGG | - | chr7_1:67420088-67420107 | None:intergenic | 30.0% | |
| TTTATGAGTATGTTTGGATC+AGG | + | chr7_1:67420441-67420460 | Msa1032480:intron | 30.0% | |
| GAAAGTCAACAAAATCATTG+TGG | - | chr7_1:67420484-67420503 | None:intergenic | 30.0% | |
| AACCGAACAATACATGTAAA+TGG | - | chr7_1:67420579-67420598 | None:intergenic | 30.0% | |
| GTAGTAAATGAAGTAGAGAT+AGG | - | chr7_1:67420088-67420107 | None:intergenic | 30.0% | |
| TTTATGAGTATGTTTGGATC+AGG | + | chr7_1:67420441-67420460 | Msa1032480:intron | 30.0% | |
| GAAAGTCAACAAAATCATTG+TGG | - | chr7_1:67420484-67420503 | None:intergenic | 30.0% | |
| AACCGAACAATACATGTAAA+TGG | - | chr7_1:67420579-67420598 | None:intergenic | 30.0% | |
| !!! | TTTTGGTGTTGTAATGTCTC+TGG | - | chr7_1:67419944-67419963 | None:intergenic | 35.0% |
| TTACAACACCAAAACAAGCA+AGG | + | chr7_1:67419950-67419969 | Msa1032480:five_prime_UTR | 35.0% | |
| TGTTTGGGGATATTGAAACT+AGG | + | chr7_1:67420157-67420176 | Msa1032480:intron | 35.0% | |
| CCATATGATCTGAATCTTGA+TGG | - | chr7_1:67420266-67420285 | None:intergenic | 35.0% | |
| CCATCAAGATTCAGATCATA+TGG | + | chr7_1:67420263-67420282 | Msa1032480:intron | 35.0% | |
| TTTGAGTCGATATTGCATGT+TGG | - | chr7_1:67420554-67420573 | None:intergenic | 35.0% | |
| GGAGACACATCTTATATCAA+TGG | + | chr7_1:67420767-67420786 | Msa1032480:CDS | 35.0% | |
| ! | TCTTAAGGACAAACGTCTTT+TGG | + | chr7_1:67420829-67420848 | Msa1032480:CDS | 35.0% |
| ATTCACACACACATTAGAAC+AGG | - | chr7_1:67421031-67421050 | None:intergenic | 35.0% | |
| !!! | TTTTGGTGTTGTAATGTCTC+TGG | - | chr7_1:67419944-67419963 | None:intergenic | 35.0% |
| TTACAACACCAAAACAAGCA+AGG | + | chr7_1:67419950-67419969 | Msa1032480:five_prime_UTR | 35.0% | |
| TGTTTGGGGATATTGAAACT+AGG | + | chr7_1:67420157-67420176 | Msa1032480:intron | 35.0% | |
| CCATATGATCTGAATCTTGA+TGG | - | chr7_1:67420266-67420285 | None:intergenic | 35.0% | |
| CCATCAAGATTCAGATCATA+TGG | + | chr7_1:67420263-67420282 | Msa1032480:intron | 35.0% | |
| TTTGAGTCGATATTGCATGT+TGG | - | chr7_1:67420554-67420573 | None:intergenic | 35.0% | |
| GGAGACACATCTTATATCAA+TGG | + | chr7_1:67420767-67420786 | Msa1032480:CDS | 35.0% | |
| ! | TCTTAAGGACAAACGTCTTT+TGG | + | chr7_1:67420829-67420848 | Msa1032480:CDS | 35.0% |
| ATTCACACACACATTAGAAC+AGG | - | chr7_1:67421031-67421050 | None:intergenic | 35.0% | |
| !! | GAGCAGTGAACAATTGGTTT+TGG | - | chr7_1:67419919-67419938 | None:intergenic | 40.0% |
| ! | GTTGAAGTCCTTGCTTGTTT+TGG | - | chr7_1:67419961-67419980 | None:intergenic | 40.0% |
| TGGACTTACATCAGACATGA+GGG | - | chr7_1:67419992-67420011 | None:intergenic | 40.0% | |
| ATGGACTTACATCAGACATG+AGG | - | chr7_1:67419993-67420012 | None:intergenic | 40.0% | |
| AAAAAAACCCATCAGGACAC+TGG | - | chr7_1:67420336-67420355 | None:intergenic | 40.0% | |
| CACTGTGAAAAAAACCCATC+AGG | - | chr7_1:67420343-67420362 | None:intergenic | 40.0% | |
| ATGCGAAGTTACATGTTCAG+AGG | - | chr7_1:67420619-67420638 | None:intergenic | 40.0% | |
| TTCGCATTGTTTGTGTGTTG+AGG | + | chr7_1:67420632-67420651 | Msa1032480:intron | 40.0% | |
| ! | AAGGACAAACGTCTTTTGGA+TGG | + | chr7_1:67420833-67420852 | Msa1032480:CDS | 40.0% |
| !! | GAGCAGTGAACAATTGGTTT+TGG | - | chr7_1:67419919-67419938 | None:intergenic | 40.0% |
| ! | GTTGAAGTCCTTGCTTGTTT+TGG | - | chr7_1:67419961-67419980 | None:intergenic | 40.0% |
| TGGACTTACATCAGACATGA+GGG | - | chr7_1:67419992-67420011 | None:intergenic | 40.0% | |
| ATGGACTTACATCAGACATG+AGG | - | chr7_1:67419993-67420012 | None:intergenic | 40.0% | |
| AAAAAAACCCATCAGGACAC+TGG | - | chr7_1:67420336-67420355 | None:intergenic | 40.0% | |
| CACTGTGAAAAAAACCCATC+AGG | - | chr7_1:67420343-67420362 | None:intergenic | 40.0% | |
| ATGCGAAGTTACATGTTCAG+AGG | - | chr7_1:67420619-67420638 | None:intergenic | 40.0% | |
| TTCGCATTGTTTGTGTGTTG+AGG | + | chr7_1:67420632-67420651 | Msa1032480:intron | 40.0% | |
| ! | AAGGACAAACGTCTTTTGGA+TGG | + | chr7_1:67420833-67420852 | Msa1032480:CDS | 40.0% |
| !! | TGGTGTTGTAATGTCTCTGG+TGG | - | chr7_1:67419941-67419960 | None:intergenic | 45.0% |
| GTATGTTTGGATCAGGACTC+TGG | + | chr7_1:67420448-67420467 | Msa1032480:intron | 45.0% | |
| !! | ATCAGGACTCTGGAATCACA+GGG | + | chr7_1:67420458-67420477 | Msa1032480:intron | 45.0% |
| ! | GCTTTGATGATTTGGCGACT+TGG | + | chr7_1:67420930-67420949 | Msa1032480:CDS | 45.0% |
| CACACACACATTAGAACAGG+AGG | - | chr7_1:67421028-67421047 | None:intergenic | 45.0% | |
| !! | TGGTGTTGTAATGTCTCTGG+TGG | - | chr7_1:67419941-67419960 | None:intergenic | 45.0% |
| GTATGTTTGGATCAGGACTC+TGG | + | chr7_1:67420448-67420467 | Msa1032480:intron | 45.0% | |
| !! | ATCAGGACTCTGGAATCACA+GGG | + | chr7_1:67420458-67420477 | Msa1032480:intron | 45.0% |
| ! | GCTTTGATGATTTGGCGACT+TGG | + | chr7_1:67420930-67420949 | Msa1032480:CDS | 45.0% |
| CACACACACATTAGAACAGG+AGG | - | chr7_1:67421028-67421047 | None:intergenic | 45.0% | |
| CTGGTGGAGCAGTGAACAAT+TGG | - | chr7_1:67419925-67419944 | None:intergenic | 50.0% | |
| !! | GATCAGGACTCTGGAATCAC+AGG | + | chr7_1:67420457-67420476 | Msa1032480:intron | 50.0% |
| CTGGTGGAGCAGTGAACAAT+TGG | - | chr7_1:67419925-67419944 | None:intergenic | 50.0% | |
| !! | GATCAGGACTCTGGAATCAC+AGG | + | chr7_1:67420457-67420476 | Msa1032480:intron | 50.0% |
| TGGAACAGCCAGTGTCCTGA+TGG | + | chr7_1:67420325-67420344 | Msa1032480:intron | 55.0% | |
| GGAACAGCCAGTGTCCTGAT+GGG | + | chr7_1:67420326-67420345 | Msa1032480:intron | 55.0% | |
| TGGAACAGCCAGTGTCCTGA+TGG | + | chr7_1:67420325-67420344 | Msa1032480:intron | 55.0% | |
| GGAACAGCCAGTGTCCTGAT+GGG | + | chr7_1:67420326-67420345 | Msa1032480:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 67419889 | 67421104 | 67419889 | ID=Msa1032480;Name=Msa1032480 |
| chr7_1 | mRNA | 67419889 | 67421104 | 67419889 | ID=Msa1032480-mRNA-1;Parent=Msa1032480;Name=Msa1032480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|1|1|2|107|96 |
| chr7_1 | exon | 67419889 | 67420002 | 67419889 | ID=Msa1032480-mRNA-1:exon:15469;Parent=Msa1032480-mRNA-1 |
| chr7_1 | exon | 67420716 | 67421104 | 67420716 | ID=Msa1032480-mRNA-1:exon:15470;Parent=Msa1032480-mRNA-1 |
| chr7_1 | five_prime_UTR | 67419889 | 67419993 | 67419889 | ID=Msa1032480-mRNA-1:five_prime_utr;Parent=Msa1032480-mRNA-1 |
| chr7_1 | CDS | 67419994 | 67420002 | 67419994 | ID=Msa1032480-mRNA-1:cds;Parent=Msa1032480-mRNA-1 |
| chr7_1 | CDS | 67420716 | 67420997 | 67420716 | ID=Msa1032480-mRNA-1:cds;Parent=Msa1032480-mRNA-1 |
| chr7_1 | three_prime_UTR | 67420998 | 67421104 | 67420998 | ID=Msa1032480-mRNA-1:three_prime_utr;Parent=Msa1032480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
