AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0870510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0870510 MtrunA17_Chr6g0453441 98.630 73 1 0 1 73 1 73 1.27e-45 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0870510 AT3G29034.1 46.575 73 38 1 2 73 14 86 2.54e-15 64.3

Find 12 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTCTGATTGGATTAAT+TGG 0.161988 6_1:-4953163 Msa0870510:CDS
GGTCTCCTTGTTCTCATGTT+GGG 0.333045 6_1:-4953076 Msa0870510:CDS
GGTTTGTGAGTAAGCAAATA+AGG 0.351967 6_1:+4953129 None:intergenic
AACACAAAAGCACTTCTGAT+TGG 0.397640 6_1:-4953172 Msa0870510:CDS
CTCATGTTGGGATTGCTTGT+AGG 0.435942 6_1:-4953064 Msa0870510:CDS
TGGTCTCCTTGTTCTCATGT+TGG 0.439787 6_1:-4953077 Msa0870510:CDS
GCTTTGACTTGGTGATATGA+AGG 0.513847 6_1:+4953108 None:intergenic
CCAAGTCAAAGCATCACAAT+TGG 0.514348 6_1:-4953097 Msa0870510:CDS
CCAATTGTGATGCTTTGACT+TGG 0.515642 6_1:+4953097 None:intergenic
TTGGGATTGCTTGTAGGAGA+AGG 0.548891 6_1:-4953058 Msa0870510:CDS
GCAATCCCAACATGAGAACA+AGG 0.621689 6_1:+4953071 None:intergenic
AAAACAAGGATGAATTACAA+AGG 0.662210 6_1:+4953028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTCTCTTCCTTCTTTTT+TGG + chr6_1:4953078-4953097 None:intergenic 30.0%
AATCCCTCATCCAAAAAAGA+AGG - chr6_1:4953065-4953084 Msa0870510:CDS 35.0%
!!! CTTCCTTCTTTTTTGGATGA+GGG + chr6_1:4953071-4953090 None:intergenic 35.0%
!!! TCTTCCTTCTTTTTTGGATG+AGG + chr6_1:4953072-4953091 None:intergenic 35.0%
!! AACACAAAAGCACTTCTGAT+TGG - chr6_1:4953111-4953130 Msa0870510:CDS 35.0%
GCACTTCTGATTGGATTAAT+TGG - chr6_1:4953120-4953139 Msa0870510:CDS 35.0%
GGTTTGTGAGTAAGCAAATA+AGG + chr6_1:4953157-4953176 None:intergenic 35.0%
!! GCTTTGACTTGGTGATATGA+AGG + chr6_1:4953178-4953197 None:intergenic 40.0%
CCAATTGTGATGCTTTGACT+TGG + chr6_1:4953189-4953208 None:intergenic 40.0%
! CCAAGTCAAAGCATCACAAT+TGG - chr6_1:4953186-4953205 Msa0870510:CDS 40.0%
TGGTCTCCTTGTTCTCATGT+TGG - chr6_1:4953206-4953225 Msa0870510:CDS 45.0%
GGTCTCCTTGTTCTCATGTT+GGG - chr6_1:4953207-4953226 Msa0870510:CDS 45.0%
GCAATCCCAACATGAGAACA+AGG + chr6_1:4953215-4953234 None:intergenic 45.0%
CTCATGTTGGGATTGCTTGT+AGG - chr6_1:4953219-4953238 Msa0870510:CDS 45.0%
TTGGGATTGCTTGTAGGAGA+AGG - chr6_1:4953225-4953244 Msa0870510:CDS 45.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 4953042 4953263 4953042 ID=Msa0870510;Name=Msa0870510
chr6_1 mRNA 4953042 4953263 4953042 ID=Msa0870510-mRNA-1;Parent=Msa0870510;Name=Msa0870510-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|73
chr6_1 exon 4953042 4953263 4953042 ID=Msa0870510-mRNA-1:exon:1574;Parent=Msa0870510-mRNA-1
chr6_1 CDS 4953042 4953263 4953042 ID=Msa0870510-mRNA-1:cds;Parent=Msa0870510-mRNA-1
Gene Sequence

>Msa0870510

ATGAAGTCAAAAAAGAAAGTTCAAATCCCTCATCCAAAAAAGAAGGAAGAGAAGAAGAAAATGAGTCAAAACACAAAAGCACTTCTGATTGGATTAATTGGTGCAGCTTTTACCTTATTTGCTTACTCACAAACCTTCATATCACCAAGTCAAAGCATCACAATTGGTCTCCTTGTTCTCATGTTGGGATTGCTTGTAGGAGAAGGTCTTATACCTTTGTAA

Protein sequence

>Msa0870510

MKSKKKVQIPHPKKKEEKKKMSQNTKALLIGLIGAAFTLFAYSQTFISPSQSITIGLLVLMLGLLVGEGLIPL*