Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1061800 | XP_003623166.1 | 97.368 | 76 | 2 | 0 | 1 | 76 | 37 | 112 | 3.12e-46 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1061800 | G7L5L7 | 97.368 | 76 | 2 | 0 | 1 | 76 | 37 | 112 | 1.49e-46 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1006750 | Msa1061800 | 0.801611 | 8.297942e-49 | -8.615850e-47 |
| Msa1061760 | Msa1061800 | 0.819234 | 1.292815e-52 | -8.615850e-47 |
| Msa1061780 | Msa1061800 | 0.825034 | 5.841701e-54 | -8.615850e-47 |
| Msa1061790 | Msa1061800 | 0.837384 | 5.366405e-57 | -8.615850e-47 |
| Msa1061800 | Msa1061810 | 0.846458 | 2.145172e-59 | -8.615850e-47 |
| Msa1061800 | Msa1150860 | 0.826670 | 2.387827e-54 | -8.615850e-47 |
| Msa1061800 | Msa1150870 | 0.813993 | 1.932159e-51 | -8.615850e-47 |
| Msa1061800 | Msa1150880 | 0.818178 | 2.244773e-52 | -8.615850e-47 |
| Msa0267710 | Msa1061800 | 0.820696 | 5.983315e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1061800 | MtrunA17_Chr7g0240471 | 97.368 | 76 | 2 | 0 | 1 | 76 | 37 | 112 | 2.87e-50 | 153 |
| Msa1061800 | MtrunA17_Chr7g0240451 | 89.333 | 75 | 8 | 0 | 2 | 76 | 38 | 112 | 3.36e-45 | 140 |
| Msa1061800 | MtrunA17_Chr7g0240441 | 71.429 | 77 | 6 | 1 | 2 | 78 | 31 | 91 | 1.98e-32 | 107 |
| Msa1061800 | MtrunA17_Chr8g0368861 | 51.316 | 76 | 36 | 1 | 3 | 77 | 41 | 116 | 6.83e-20 | 77.0 |
| Msa1061800 | MtrunA17_Chr8g0368831 | 51.316 | 76 | 36 | 1 | 3 | 77 | 49 | 124 | 7.18e-20 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1061800 | AT3G53980.2 | 69.333 | 75 | 23 | 0 | 2 | 76 | 35 | 109 | 1.20e-35 | 117 |
| Msa1061800 | AT3G53980.1 | 69.333 | 75 | 23 | 0 | 2 | 76 | 35 | 109 | 1.20e-35 | 117 |
| Msa1061800 | AT5G05960.1 | 68.000 | 75 | 24 | 0 | 2 | 76 | 37 | 111 | 9.98e-34 | 112 |
| Msa1061800 | AT2G37870.1 | 50.000 | 76 | 37 | 1 | 3 | 77 | 36 | 111 | 8.96e-20 | 76.6 |
Find 11 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7_2:+52155394 | None:intergenic |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.339318 | 7_2:+52155393 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7_2:-52155444 | Msa1061800:CDS |
| AACTTTGCTAATCGTCCCAT+TGG | 0.509591 | 7_2:-52155381 | Msa1061800:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.522424 | 7_2:+52155414 | None:intergenic |
| CGTCCGCACTTATAACCAAT+GGG | 0.524534 | 7_2:+52155366 | None:intergenic |
| ACGTCCGCACTTATAACCAA+TGG | 0.525340 | 7_2:+52155365 | None:intergenic |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7_2:+52155552 | None:intergenic |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7_2:+52155471 | None:intergenic |
| TGTGCTCAGACTAAGAAACT+TGG | 0.591888 | 7_2:-52155501 | Msa1061800:CDS |
| CGTCCCATTGGTTATAAGTG+CGG | 0.667176 | 7_2:-52155369 | Msa1061800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACTGCACAAAGACAACT+TGG | + | chr7_2:52155464-52155483 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | - | chr7_2:52155488-52155507 | Msa1061800:CDS | 35.0% | |
| TGTGCTCAGACTAAGAAACT+TGG | - | chr7_2:52155431-52155450 | Msa1061800:CDS | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | + | chr7_2:52155521-52155540 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | + | chr7_2:52155541-52155560 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | + | chr7_2:52155542-52155561 | None:intergenic | 40.0% | |
| AACTTTGCTAATCGTCCCAT+TGG | - | chr7_2:52155551-52155570 | Msa1061800:CDS | 40.0% | |
| ! | CGTCCCATTGGTTATAAGTG+CGG | - | chr7_2:52155563-52155582 | Msa1061800:CDS | 45.0% |
| CGTCCGCACTTATAACCAAT+GGG | + | chr7_2:52155569-52155588 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | + | chr7_2:52155570-52155589 | None:intergenic | 45.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | + | chr7_2:52155383-52155402 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 52155359 | 52155595 | 52155359 | ID=Msa1061800;Name=Msa1061800 |
| chr7_2 | mRNA | 52155359 | 52155595 | 52155359 | ID=Msa1061800-mRNA-1;Parent=Msa1061800;Name=Msa1061800-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|78 |
| chr7_2 | exon | 52155359 | 52155595 | 52155359 | ID=Msa1061800-mRNA-1:exon:9418;Parent=Msa1061800-mRNA-1 |
| chr7_2 | CDS | 52155359 | 52155595 | 52155359 | ID=Msa1061800-mRNA-1:cds;Parent=Msa1061800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
