AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1061760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061760 MtrunA17_Chr7g0240451 95.726 117 5 0 1 117 1 117 6.12e-80 230
Msa1061760 MtrunA17_Chr7g0240471 87.179 117 15 0 1 117 1 117 5.33e-73 213
Msa1061760 MtrunA17_Chr7g0240441 74.026 77 4 1 36 112 29 89 1.27e-33 112
Msa1061760 MtrunA17_Chr8g0368861 45.455 121 61 3 1 117 1 120 3.05e-25 92.0
Msa1061760 MtrunA17_Chr8g0368831 44.628 121 62 3 1 117 9 128 1.43e-24 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061760 AT5G05960.1 61.261 111 36 2 7 117 13 116 1.10e-42 136
Msa1061760 AT3G53980.2 64.211 95 33 1 23 117 21 114 2.97e-42 135
Msa1061760 AT3G53980.1 64.211 95 33 1 23 117 21 114 2.97e-42 135
Msa1061760 AT2G37870.1 49.107 112 51 4 10 117 6 115 5.51e-26 94.0

Find 38 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTTGGTATGGATTTAATT+TGG 0.228589 7_2:+52111524 Msa1061760:three_prime_UTR
CAGCAAGGTTGCAACGTTTA+GGG 0.315042 7_2:-52110882 None:intergenic
GTTGCTGTTAGCTGCTATTA+TGG 0.326649 7_2:+52111319 Msa1061760:three_prime_UTR
TCAGCAAGGTTGCAACGTTT+AGG 0.352849 7_2:-52110883 None:intergenic
GGTATTGCAGTTCTTGTTGC+AGG 0.361788 7_2:+52110628 Msa1061760:CDS
GGGTTACTATTTAATATAGT+TGG 0.381173 7_2:+52111508 Msa1061760:three_prime_UTR
CAACAACCTCAACTCTTTCA+AGG 0.398597 7_2:-52111266 None:intergenic
AGCTTAAAAGCTTCATTGTC+TGG 0.411064 7_2:-52110697 None:intergenic
ATGCAACAATCCCATGCATT+AGG 0.423611 7_2:-52110527 None:intergenic
ACTATTTAATATAGTTGGTA+TGG 0.424897 7_2:+52111513 Msa1061760:three_prime_UTR
TCAAACGTTGCTAAAATGTC+TGG 0.431901 7_2:+52110832 Msa1061760:CDS
GGATAGTAACAGCAATTTGA+GGG 0.472152 7_2:-52110861 None:intergenic
TTGCTGAAAACAAAGATAAG+AGG 0.488652 7_2:+52111286 Msa1061760:three_prime_UTR
TGTGCTCAGGTTAAGAAACT+TGG 0.492815 7_2:+52110775 Msa1061760:CDS
GTGAAATTGTTGTGCTTCGT+TGG 0.500117 7_2:+52110607 Msa1061760:CDS
GGGATAGTAACAGCAATTTG+AGG 0.508534 7_2:-52110862 None:intergenic
TTATAGTACTAAAATGTGGT+TGG 0.510447 7_2:-52110497 None:intergenic
GTCACAAAATTGTTGTGCTC+AGG 0.514410 7_2:+52110762 Msa1061760:CDS
CGTCCACACTTGTAACCGAC+AGG 0.520932 7_2:-52110910 None:intergenic
CAACAATCCCATGCATTAGG+TGG 0.523179 7_2:-52110524 None:intergenic
CGCCCTGTCGGTTACAAGTG+TGG 0.534054 7_2:+52110907 Msa1061760:CDS
GTTGAAAGTGCTGGTGAATG+TGG 0.539242 7_2:+52110661 Msa1061760:CDS
TAACTTTACTGTTTATAGTG+TGG 0.540728 7_2:+52111369 Msa1061760:three_prime_UTR
AAAGTGCTGGTGAATGTGGA+AGG 0.549386 7_2:+52110665 Msa1061760:CDS
AACCTTGCTGATCGCCCTGT+CGG 0.551684 7_2:+52110895 Msa1061760:CDS
ATTCATAGAGTTGAAAGTGC+TGG 0.554882 7_2:+52110652 Msa1061760:CDS
ATAAAATCCCACCTAATGCA+TGG 0.561702 7_2:+52110516 Msa1061760:five_prime_UTR
TAAAATCCCACCTAATGCAT+GGG 0.561751 7_2:+52110517 Msa1061760:five_prime_UTR
ACTCTTCCTTGAAAGAGTTG+AGG 0.566469 7_2:+52111260 Msa1061760:exon
TGCTGAAAACAAAGATAAGA+GGG 0.575968 7_2:+52111287 Msa1061760:three_prime_UTR
ATAACAGCACAAAGACAACT+TGG 0.604458 7_2:-52110805 None:intergenic
AACAATCCCATGCATTAGGT+GGG 0.609272 7_2:-52110523 None:intergenic
TTTATTCAGGGTGTTCACAA+AGG 0.622018 7_2:-52111408 None:intergenic
AGTGCTGGTGAATGTGGAAG+GGG 0.630405 7_2:+52110667 Msa1061760:CDS
AACCGACAGGGCGATCAGCA+AGG 0.631783 7_2:-52110897 None:intergenic
AAGTGCTGGTGAATGTGGAA+GGG 0.641454 7_2:+52110666 Msa1061760:CDS
AATACAACACAACTAAGCTG+TGG 0.678775 7_2:-52111345 None:intergenic
GTCCACACTTGTAACCGACA+GGG 0.709589 7_2:-52110909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAGAAGAAAAAAAAA+TGG + chr7_2:52110576-52110595 Msa1061760:exon 10.0%
!!! TAATTATTTATCTTTTTTCT+TGG + chr7_2:52111184-52111203 Msa1061760:intron 10.0%
!! ATATTAAGAAGAAAAAAAAA+TGG + chr7_2:52110576-52110595 Msa1061760:exon 10.0%
!!! TAATTATTTATCTTTTTTCT+TGG + chr7_2:52111184-52111203 Msa1061760:intron 10.0%
!!! TACTTATTTGACATTTTTTT+AGG - chr7_2:52111146-52111165 None:intergenic 15.0%
!!! TACTTATTTGACATTTTTTT+AGG - chr7_2:52111146-52111165 None:intergenic 15.0%
!!! GATTTTATAGTACTAAAATG+TGG - chr7_2:52110504-52110523 None:intergenic 20.0%
!! TTAAGAAGAAAAAAAAATGG+AGG + chr7_2:52110579-52110598 Msa1061760:exon 20.0%
!! TAAGAAGAAAAAAAAATGGA+GGG + chr7_2:52110580-52110599 Msa1061760:exon 20.0%
!!! AAATGAAACTGTTTTATTCA+GGG - chr7_2:52111423-52111442 None:intergenic 20.0%
!!! AAAATGAAACTGTTTTATTC+AGG - chr7_2:52111424-52111443 None:intergenic 20.0%
!!! GATGCTTTTACATTATATTT+GGG + chr7_2:52111483-52111502 Msa1061760:three_prime_UTR 20.0%
!!! TTTTACATTATATTTGGGTT+GGG + chr7_2:52111488-52111507 Msa1061760:three_prime_UTR 20.0%
!!! ACTATTTAATATAGTTGGTA+TGG + chr7_2:52111513-52111532 Msa1061760:three_prime_UTR 20.0%
!!! GATTTTATAGTACTAAAATG+TGG - chr7_2:52110504-52110523 None:intergenic 20.0%
!! TTAAGAAGAAAAAAAAATGG+AGG + chr7_2:52110579-52110598 Msa1061760:exon 20.0%
!! TAAGAAGAAAAAAAAATGGA+GGG + chr7_2:52110580-52110599 Msa1061760:exon 20.0%
!!! AAATGAAACTGTTTTATTCA+GGG - chr7_2:52111423-52111442 None:intergenic 20.0%
!!! AAAATGAAACTGTTTTATTC+AGG - chr7_2:52111424-52111443 None:intergenic 20.0%
!!! GATGCTTTTACATTATATTT+GGG + chr7_2:52111483-52111502 Msa1061760:three_prime_UTR 20.0%
!!! TTTTACATTATATTTGGGTT+GGG + chr7_2:52111488-52111507 Msa1061760:three_prime_UTR 20.0%
!!! ACTATTTAATATAGTTGGTA+TGG + chr7_2:52111513-52111532 Msa1061760:three_prime_UTR 20.0%
! TTATAGTACTAAAATGTGGT+TGG - chr7_2:52110500-52110519 None:intergenic 25.0%
! TAACTTTACTGTTTATAGTG+TGG + chr7_2:52111369-52111388 Msa1061760:three_prime_UTR 25.0%
!! GGATGCTTTTACATTATATT+TGG + chr7_2:52111482-52111501 Msa1061760:three_prime_UTR 25.0%
!! CTTTTACATTATATTTGGGT+TGG + chr7_2:52111487-52111506 Msa1061760:three_prime_UTR 25.0%
! GGGTTACTATTTAATATAGT+TGG + chr7_2:52111508-52111527 Msa1061760:three_prime_UTR 25.0%
!! TAGTTGGTATGGATTTAATT+TGG + chr7_2:52111524-52111543 Msa1061760:three_prime_UTR 25.0%
! TTATAGTACTAAAATGTGGT+TGG - chr7_2:52110500-52110519 None:intergenic 25.0%
! TAACTTTACTGTTTATAGTG+TGG + chr7_2:52111369-52111388 Msa1061760:three_prime_UTR 25.0%
!! GGATGCTTTTACATTATATT+TGG + chr7_2:52111482-52111501 Msa1061760:three_prime_UTR 25.0%
!! CTTTTACATTATATTTGGGT+TGG + chr7_2:52111487-52111506 Msa1061760:three_prime_UTR 25.0%
! GGGTTACTATTTAATATAGT+TGG + chr7_2:52111508-52111527 Msa1061760:three_prime_UTR 25.0%
!! TAGTTGGTATGGATTTAATT+TGG + chr7_2:52111524-52111543 Msa1061760:three_prime_UTR 25.0%
TTGCTGAAAACAAAGATAAG+AGG + chr7_2:52111286-52111305 Msa1061760:three_prime_UTR 30.0%
TGCTGAAAACAAAGATAAGA+GGG + chr7_2:52111287-52111306 Msa1061760:three_prime_UTR 30.0%
!!! ATCCACTAAAAAGTTTTGGA+AGG - chr7_2:52111466-52111485 None:intergenic 30.0%
! AAGCATCCACTAAAAAGTTT+TGG - chr7_2:52111470-52111489 None:intergenic 30.0%
TTGCTGAAAACAAAGATAAG+AGG + chr7_2:52111286-52111305 Msa1061760:three_prime_UTR 30.0%
TGCTGAAAACAAAGATAAGA+GGG + chr7_2:52111287-52111306 Msa1061760:three_prime_UTR 30.0%
!!! ATCCACTAAAAAGTTTTGGA+AGG - chr7_2:52111466-52111485 None:intergenic 30.0%
! AAGCATCCACTAAAAAGTTT+TGG - chr7_2:52111470-52111489 None:intergenic 30.0%
ATAAAATCCCACCTAATGCA+TGG + chr7_2:52110516-52110535 Msa1061760:five_prime_UTR 35.0%
TAAAATCCCACCTAATGCAT+GGG + chr7_2:52110517-52110536 Msa1061760:five_prime_UTR 35.0%
! ATTCATAGAGTTGAAAGTGC+TGG + chr7_2:52110652-52110671 Msa1061760:CDS 35.0%
AGCTTAAAAGCTTCATTGTC+TGG - chr7_2:52110700-52110719 None:intergenic 35.0%
ATAACAGCACAAAGACAACT+TGG - chr7_2:52110808-52110827 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7_2:52110832-52110851 Msa1061760:CDS 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7_2:52110864-52110883 None:intergenic 35.0%
AATACAACACAACTAAGCTG+TGG - chr7_2:52111348-52111367 None:intergenic 35.0%
! TTTATTCAGGGTGTTCACAA+AGG - chr7_2:52111411-52111430 None:intergenic 35.0%
! TGCCTTCCAAAACTTTTTAG+TGG + chr7_2:52111461-52111480 Msa1061760:three_prime_UTR 35.0%
ATAAAATCCCACCTAATGCA+TGG + chr7_2:52110516-52110535 Msa1061760:five_prime_UTR 35.0%
TAAAATCCCACCTAATGCAT+GGG + chr7_2:52110517-52110536 Msa1061760:five_prime_UTR 35.0%
! ATTCATAGAGTTGAAAGTGC+TGG + chr7_2:52110652-52110671 Msa1061760:CDS 35.0%
AGCTTAAAAGCTTCATTGTC+TGG - chr7_2:52110700-52110719 None:intergenic 35.0%
ATAACAGCACAAAGACAACT+TGG - chr7_2:52110808-52110827 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7_2:52110832-52110851 Msa1061760:CDS 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7_2:52110864-52110883 None:intergenic 35.0%
AATACAACACAACTAAGCTG+TGG - chr7_2:52111348-52111367 None:intergenic 35.0%
! TTTATTCAGGGTGTTCACAA+AGG - chr7_2:52111411-52111430 None:intergenic 35.0%
! TGCCTTCCAAAACTTTTTAG+TGG + chr7_2:52111461-52111480 Msa1061760:three_prime_UTR 35.0%
AACAATCCCATGCATTAGGT+GGG - chr7_2:52110526-52110545 None:intergenic 40.0%
ATGCAACAATCCCATGCATT+AGG - chr7_2:52110530-52110549 None:intergenic 40.0%
GTGAAATTGTTGTGCTTCGT+TGG + chr7_2:52110607-52110626 Msa1061760:CDS 40.0%
GTCACAAAATTGTTGTGCTC+AGG + chr7_2:52110762-52110781 Msa1061760:CDS 40.0%
TGTGCTCAGGTTAAGAAACT+TGG + chr7_2:52110775-52110794 Msa1061760:CDS 40.0%
GGGATAGTAACAGCAATTTG+AGG - chr7_2:52110865-52110884 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG + chr7_2:52111260-52111279 Msa1061760:exon 40.0%
CAACAACCTCAACTCTTTCA+AGG - chr7_2:52111269-52111288 None:intergenic 40.0%
! GTTGCTGTTAGCTGCTATTA+TGG + chr7_2:52111319-52111338 Msa1061760:three_prime_UTR 40.0%
AACAATCCCATGCATTAGGT+GGG - chr7_2:52110526-52110545 None:intergenic 40.0%
ATGCAACAATCCCATGCATT+AGG - chr7_2:52110530-52110549 None:intergenic 40.0%
GTGAAATTGTTGTGCTTCGT+TGG + chr7_2:52110607-52110626 Msa1061760:CDS 40.0%
GTCACAAAATTGTTGTGCTC+AGG + chr7_2:52110762-52110781 Msa1061760:CDS 40.0%
TGTGCTCAGGTTAAGAAACT+TGG + chr7_2:52110775-52110794 Msa1061760:CDS 40.0%
GGGATAGTAACAGCAATTTG+AGG - chr7_2:52110865-52110884 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG + chr7_2:52111260-52111279 Msa1061760:exon 40.0%
CAACAACCTCAACTCTTTCA+AGG - chr7_2:52111269-52111288 None:intergenic 40.0%
! GTTGCTGTTAGCTGCTATTA+TGG + chr7_2:52111319-52111338 Msa1061760:three_prime_UTR 40.0%
CAACAATCCCATGCATTAGG+TGG - chr7_2:52110527-52110546 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG + chr7_2:52110628-52110647 Msa1061760:CDS 45.0%
!! GTTGAAAGTGCTGGTGAATG+TGG + chr7_2:52110661-52110680 Msa1061760:CDS 45.0%
!! AAAGTGCTGGTGAATGTGGA+AGG + chr7_2:52110665-52110684 Msa1061760:CDS 45.0%
!! AAGTGCTGGTGAATGTGGAA+GGG + chr7_2:52110666-52110685 Msa1061760:CDS 45.0%
! TCTTTTGCTGCACTTGCACA+AGG - chr7_2:52110727-52110746 None:intergenic 45.0%
CAGCAAGGTTGCAACGTTTA+GGG - chr7_2:52110885-52110904 None:intergenic 45.0%
TCAGCAAGGTTGCAACGTTT+AGG - chr7_2:52110886-52110905 None:intergenic 45.0%
CAACAATCCCATGCATTAGG+TGG - chr7_2:52110527-52110546 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG + chr7_2:52110628-52110647 Msa1061760:CDS 45.0%
!! GTTGAAAGTGCTGGTGAATG+TGG + chr7_2:52110661-52110680 Msa1061760:CDS 45.0%
!! AAAGTGCTGGTGAATGTGGA+AGG + chr7_2:52110665-52110684 Msa1061760:CDS 45.0%
!! AAGTGCTGGTGAATGTGGAA+GGG + chr7_2:52110666-52110685 Msa1061760:CDS 45.0%
! TCTTTTGCTGCACTTGCACA+AGG - chr7_2:52110727-52110746 None:intergenic 45.0%
CAGCAAGGTTGCAACGTTTA+GGG - chr7_2:52110885-52110904 None:intergenic 45.0%
TCAGCAAGGTTGCAACGTTT+AGG - chr7_2:52110886-52110905 None:intergenic 45.0%
!! AGTGCTGGTGAATGTGGAAG+GGG + chr7_2:52110667-52110686 Msa1061760:CDS 50.0%
GTCCACACTTGTAACCGACA+GGG - chr7_2:52110912-52110931 None:intergenic 50.0%
!! AGTGCTGGTGAATGTGGAAG+GGG + chr7_2:52110667-52110686 Msa1061760:CDS 50.0%
GTCCACACTTGTAACCGACA+GGG - chr7_2:52110912-52110931 None:intergenic 50.0%
AACCTTGCTGATCGCCCTGT+CGG + chr7_2:52110895-52110914 Msa1061760:CDS 55.0%
CGTCCACACTTGTAACCGAC+AGG - chr7_2:52110913-52110932 None:intergenic 55.0%
AACCTTGCTGATCGCCCTGT+CGG + chr7_2:52110895-52110914 Msa1061760:CDS 55.0%
CGTCCACACTTGTAACCGAC+AGG - chr7_2:52110913-52110932 None:intergenic 55.0%
AACCGACAGGGCGATCAGCA+AGG - chr7_2:52110900-52110919 None:intergenic 60.0%
! CGCCCTGTCGGTTACAAGTG+TGG + chr7_2:52110907-52110926 Msa1061760:CDS 60.0%
AACCGACAGGGCGATCAGCA+AGG - chr7_2:52110900-52110919 None:intergenic 60.0%
! CGCCCTGTCGGTTACAAGTG+TGG + chr7_2:52110907-52110926 Msa1061760:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 52110495 52111595 52110495 ID=Msa1061760;Name=Msa1061760
chr7_2 mRNA 52110495 52111595 52110495 ID=Msa1061760-mRNA-1;Parent=Msa1061760;Name=Msa1061760-mRNA-1;_AED=0.23;_eAED=0.23;_QI=100|1|1|1|1|1|2|324|117
chr7_2 exon 52110495 52110931 52110495 ID=Msa1061760-mRNA-1:exon:9403;Parent=Msa1061760-mRNA-1
chr7_2 exon 52111255 52111595 52111255 ID=Msa1061760-mRNA-1:exon:9404;Parent=Msa1061760-mRNA-1
chr7_2 five_prime_UTR 52110495 52110594 52110495 ID=Msa1061760-mRNA-1:five_prime_utr;Parent=Msa1061760-mRNA-1
chr7_2 CDS 52110595 52110931 52110595 ID=Msa1061760-mRNA-1:cds;Parent=Msa1061760-mRNA-1
chr7_2 CDS 52111255 52111271 52111255 ID=Msa1061760-mRNA-1:cds;Parent=Msa1061760-mRNA-1
chr7_2 three_prime_UTR 52111272 52111595 52111272 ID=Msa1061760-mRNA-1:three_prime_utr;Parent=Msa1061760-mRNA-1
Gene Sequence

>Msa1061760

ATGGAGGGTCTTGTGAAATTGTTGTGCTTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCTGGTGAATGTGGAAGGGGTACTACACCAGACAATGAAGCTTTTAAGCTACTTCCTTGTGCAAGTGCAGCAAAAGATGAAAATGCTAGTGTGTCACAAAATTGTTGTGCTCAGGTTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCTGTTATGCTTTCAAACGTTGCTAAAATGTCTGGTGCTAACCCTCAAATTGCTGTTACTATCCCTAAACGTTGCAACCTTGCTGATCGCCCTGTCGGTTACAAGTGTGGACCTTACACTCTTCCTTGA

Protein sequence

>Msa1061760

MEGLVKLLCFVGIAVLVAGIHRVESAGECGRGTTPDNEAFKLLPCASAAKDENASVSQNCCAQVKKLGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCGPYTLP*