AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1061780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061780 MtrunA17_Chr7g0240471 90.625 96 9 0 1 96 1 96 5.31e-60 179
Msa1061780 MtrunA17_Chr7g0240451 87.500 96 12 0 1 96 1 96 1.16e-57 174
Msa1061780 MtrunA17_Chr7g0240441 65.574 61 5 1 36 96 29 73 3.91e-18 73.2
Msa1061780 MtrunA17_Chr8g0368861 39.000 100 56 3 1 96 1 99 3.47e-12 58.5
Msa1061780 MtrunA17_Chr8g0368831 38.000 100 57 3 1 96 9 107 1.60e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061780 AT3G53980.2 62.162 74 27 1 23 96 21 93 8.98e-28 98.6
Msa1061780 AT3G53980.1 62.162 74 27 1 23 96 21 93 8.98e-28 98.6
Msa1061780 AT5G05960.1 49.462 93 40 2 4 96 10 95 1.43e-25 93.2
Msa1061780 AT2G37870.1 40.909 88 50 2 8 94 6 92 6.47e-14 63.2

Find 24 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTGAAAACAAACATAAA+TGG 0.229535 7_2:-52131415 Msa1061780:CDS
AGCTTAAATGCTTCCATATC+TGG 0.265044 7_2:+52132024 None:intergenic
GATGCTGTTAGCTACTATTA+TGG 0.319174 7_2:-52131382 Msa1061780:three_prime_UTR
GGTATTGCAGTTCTTGTTGC+AGG 0.361788 7_2:-52132093 Msa1061780:CDS
CAACAACCTCAACTCTTTCA+AGG 0.398597 7_2:+52131435 None:intergenic
TGCTGAAAACAAACATAAAT+GGG 0.434655 7_2:-52131414 Msa1061780:CDS
TCAAACGTTGCTAAAATGTC+TGG 0.462464 7_2:-52131889 Msa1061780:CDS
AAGGGGTACTACACCAGATA+TGG 0.470603 7_2:-52132037 Msa1061780:CDS
GGATAGTAACAGCAATTTGA+GGG 0.472152 7_2:+52131860 None:intergenic
GGGATAGTAACAGCAATTTG+AGG 0.508534 7_2:+52131859 None:intergenic
CGAGGCAAAACAATTTCATA+AGG 0.511907 7_2:+52132118 None:intergenic
GTTGAAAGTGCAGGTGAATG+TGG 0.515102 7_2:-52132060 Msa1061780:CDS
TCTTGTGCTGCACTTGCACA+AGG 0.541464 7_2:+52131997 None:intergenic
AAAGTGCAGGTGAATGTGGA+AGG 0.549386 7_2:-52132056 Msa1061780:CDS
ACTCTTCCTTGAAAGAGTTG+AGG 0.566469 7_2:-52131441 Msa1061780:CDS
TGTGATCAGACTAAGAAACT+TGG 0.574065 7_2:-52131946 Msa1061780:CDS
ATTCATAGAGTTGAAAGTGC+AGG 0.578390 7_2:-52132069 Msa1061780:CDS
ATCTTTATCAAGAGTAGTAA+CGG 0.588821 7_2:+52131179 None:intergenic
AAAACTGCACAAAGACAACT+TGG 0.590774 7_2:+52131916 None:intergenic
AATACAACACAACTAAGTTG+TGG 0.625133 7_2:+52131356 None:intergenic
GTGTACACAAGTGAGCTGCT+AGG 0.629312 7_2:+52131299 None:intergenic
AGTGCAGGTGAATGTGGAAG+GGG 0.630405 7_2:-52132054 Msa1061780:CDS
AAGTGCAGGTGAATGTGGAA+GGG 0.641454 7_2:-52132055 Msa1061780:CDS
CAAGAACTGCAATACCAACG+AGG 0.744882 7_2:+52132100 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAACTTTTATATGAAAA+CGG + chr7_2:52131496-52131515 None:intergenic 10.0%
!!! TTTTAACAATAAATTTTCTT+AGG + chr7_2:52131623-52131642 None:intergenic 10.0%
!!! AAATAACTTTTATATGAAAA+CGG + chr7_2:52131496-52131515 None:intergenic 10.0%
!!! TTTTAACAATAAATTTTCTT+AGG + chr7_2:52131623-52131642 None:intergenic 10.0%
!!! GTTTTCATATAAAAGTTATT+TGG - chr7_2:52131495-52131514 Msa1061780:intron 15.0%
!! ATGTAACATTAATAATTGAT+AGG - chr7_2:52131548-52131567 Msa1061780:intron 15.0%
!!! GTTTTCATATAAAAGTTATT+TGG - chr7_2:52131495-52131514 Msa1061780:intron 15.0%
!! ATGTAACATTAATAATTGAT+AGG - chr7_2:52131548-52131567 Msa1061780:intron 15.0%
!! AATGAGATTATAGATAGTTA+AGG - chr7_2:52131574-52131593 Msa1061780:intron 20.0%
!! ATGAGATTATAGATAGTTAA+GGG - chr7_2:52131575-52131594 Msa1061780:intron 20.0%
!!! AATAAAACATACTCTTTTAG+TGG - chr7_2:52131975-52131994 Msa1061780:CDS 20.0%
!!! AGTATTTTTAGAATTTGAGA+TGG + chr7_2:52132091-52132110 None:intergenic 20.0%
!! AATGAGATTATAGATAGTTA+AGG - chr7_2:52131574-52131593 Msa1061780:intron 20.0%
!! ATGAGATTATAGATAGTTAA+GGG - chr7_2:52131575-52131594 Msa1061780:intron 20.0%
!!! AATAAAACATACTCTTTTAG+TGG - chr7_2:52131975-52131994 Msa1061780:CDS 20.0%
!!! AGTATTTTTAGAATTTGAGA+TGG + chr7_2:52132091-52132110 None:intergenic 20.0%
!!! TAGATAGTTAAGGGTATTAT+AGG - chr7_2:52131584-52131603 Msa1061780:intron 25.0%
! TTGTTAAAATCTTCCCTTTA+TGG - chr7_2:52131634-52131653 Msa1061780:intron 25.0%
! TTGCTGAAAACAAACATAAA+TGG - chr7_2:52131829-52131848 Msa1061780:intron 25.0%
! TGCTGAAAACAAACATAAAT+GGG - chr7_2:52131830-52131849 Msa1061780:intron 25.0%
!!! AGAGTATGTTTTATTCATGA+GGG + chr7_2:52131970-52131989 None:intergenic 25.0%
!!! AAGAGTATGTTTTATTCATG+AGG + chr7_2:52131971-52131990 None:intergenic 25.0%
!! TTCCTACTAAAACATTTTAG+TGG - chr7_2:52132009-52132028 Msa1061780:CDS 25.0%
! ATCTTTATCAAGAGTAGTAA+CGG + chr7_2:52132068-52132087 None:intergenic 25.0%
!! TGAATTTTGTTAAAGATGTG+AGG - chr7_2:52132118-52132137 Msa1061780:CDS 25.0%
!!! TAGATAGTTAAGGGTATTAT+AGG - chr7_2:52131584-52131603 Msa1061780:intron 25.0%
! TTGTTAAAATCTTCCCTTTA+TGG - chr7_2:52131634-52131653 Msa1061780:intron 25.0%
! TTGCTGAAAACAAACATAAA+TGG - chr7_2:52131829-52131848 Msa1061780:intron 25.0%
! TGCTGAAAACAAACATAAAT+GGG - chr7_2:52131830-52131849 Msa1061780:intron 25.0%
!!! AGAGTATGTTTTATTCATGA+GGG + chr7_2:52131970-52131989 None:intergenic 25.0%
!!! AAGAGTATGTTTTATTCATG+AGG + chr7_2:52131971-52131990 None:intergenic 25.0%
!! TTCCTACTAAAACATTTTAG+TGG - chr7_2:52132009-52132028 Msa1061780:CDS 25.0%
! ATCTTTATCAAGAGTAGTAA+CGG + chr7_2:52132068-52132087 None:intergenic 25.0%
!! TGAATTTTGTTAAAGATGTG+AGG - chr7_2:52132118-52132137 Msa1061780:CDS 25.0%
!! CTAGTTTCTAGTTCCATAAA+GGG + chr7_2:52131650-52131669 None:intergenic 30.0%
!! TCTAGTTTCTAGTTCCATAA+AGG + chr7_2:52131651-52131670 None:intergenic 30.0%
TGCCTAAGAAAAATGTGAAA+CGG - chr7_2:52131681-52131700 Msa1061780:intron 30.0%
! TACCGTTTCACATTTTTCTT+AGG + chr7_2:52131686-52131705 None:intergenic 30.0%
! CTACTTGTCAATTTTCTTGA+TGG - chr7_2:52131734-52131753 Msa1061780:intron 30.0%
AATACAACACAACTAAGTTG+TGG + chr7_2:52131891-52131910 None:intergenic 30.0%
!!! GACCACTAAAATGTTTTAGT+AGG + chr7_2:52132014-52132033 None:intergenic 30.0%
!! AAAACATTTTAGTGGTCTAG+TGG - chr7_2:52132017-52132036 Msa1061780:CDS 30.0%
!! CTAGTTTCTAGTTCCATAAA+GGG + chr7_2:52131650-52131669 None:intergenic 30.0%
!! TCTAGTTTCTAGTTCCATAA+AGG + chr7_2:52131651-52131670 None:intergenic 30.0%
TGCCTAAGAAAAATGTGAAA+CGG - chr7_2:52131681-52131700 Msa1061780:intron 30.0%
! TACCGTTTCACATTTTTCTT+AGG + chr7_2:52131686-52131705 None:intergenic 30.0%
! CTACTTGTCAATTTTCTTGA+TGG - chr7_2:52131734-52131753 Msa1061780:intron 30.0%
AATACAACACAACTAAGTTG+TGG + chr7_2:52131891-52131910 None:intergenic 30.0%
!!! GACCACTAAAATGTTTTAGT+AGG + chr7_2:52132014-52132033 None:intergenic 30.0%
!! AAAACATTTTAGTGGTCTAG+TGG - chr7_2:52132017-52132036 Msa1061780:CDS 30.0%
CGAGGCAAAACAATTTCATA+AGG + chr7_2:52131129-52131148 None:intergenic 35.0%
!! ATGAAATTGTTTTGCCTCGT+TGG - chr7_2:52131130-52131149 Msa1061780:three_prime_UTR 35.0%
! ATTCATAGAGTTGAAAGTGC+AGG - chr7_2:52131175-52131194 Msa1061780:three_prime_UTR 35.0%
AGCTTAAATGCTTCCATATC+TGG + chr7_2:52131223-52131242 None:intergenic 35.0%
TGTGATCAGACTAAGAAACT+TGG - chr7_2:52131298-52131317 Msa1061780:three_prime_UTR 35.0%
AAAACTGCACAAAGACAACT+TGG + chr7_2:52131331-52131350 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG - chr7_2:52131355-52131374 Msa1061780:three_prime_UTR 35.0%
GGATAGTAACAGCAATTTGA+GGG + chr7_2:52131387-52131406 None:intergenic 35.0%
TAAGTAACTAACAAGTCCCT+CGG - chr7_2:52131456-52131475 Msa1061780:intron 35.0%
GATGCTGTTAGCTACTATTA+TGG - chr7_2:52131862-52131881 Msa1061780:intron 35.0%
CGAGGCAAAACAATTTCATA+AGG + chr7_2:52131129-52131148 None:intergenic 35.0%
!! ATGAAATTGTTTTGCCTCGT+TGG - chr7_2:52131130-52131149 Msa1061780:three_prime_UTR 35.0%
! ATTCATAGAGTTGAAAGTGC+AGG - chr7_2:52131175-52131194 Msa1061780:three_prime_UTR 35.0%
AGCTTAAATGCTTCCATATC+TGG + chr7_2:52131223-52131242 None:intergenic 35.0%
TGTGATCAGACTAAGAAACT+TGG - chr7_2:52131298-52131317 Msa1061780:three_prime_UTR 35.0%
AAAACTGCACAAAGACAACT+TGG + chr7_2:52131331-52131350 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG - chr7_2:52131355-52131374 Msa1061780:three_prime_UTR 35.0%
GGATAGTAACAGCAATTTGA+GGG + chr7_2:52131387-52131406 None:intergenic 35.0%
TAAGTAACTAACAAGTCCCT+CGG - chr7_2:52131456-52131475 Msa1061780:intron 35.0%
GATGCTGTTAGCTACTATTA+TGG - chr7_2:52131862-52131881 Msa1061780:intron 35.0%
GGGATAGTAACAGCAATTTG+AGG + chr7_2:52131388-52131407 None:intergenic 40.0%
TAGCAAAGTTGCAGCGTTTA+GGG + chr7_2:52131408-52131427 None:intergenic 40.0%
TTAGCAAAGTTGCAGCGTTT+AGG + chr7_2:52131409-52131428 None:intergenic 40.0%
GTCCACACTTATAACCAATG+GGG + chr7_2:52131435-52131454 None:intergenic 40.0%
CGTCCACACTTATAACCAAT+GGG + chr7_2:52131436-52131455 None:intergenic 40.0%
ACGTCCACACTTATAACCAA+TGG + chr7_2:52131437-52131456 None:intergenic 40.0%
GGCAACTTAAAAGAAACCGA+GGG + chr7_2:52131475-52131494 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG - chr7_2:52131803-52131822 Msa1061780:intron 40.0%
CAACAACCTCAACTCTTTCA+AGG + chr7_2:52131812-52131831 None:intergenic 40.0%
GGGATAGTAACAGCAATTTG+AGG + chr7_2:52131388-52131407 None:intergenic 40.0%
TAGCAAAGTTGCAGCGTTTA+GGG + chr7_2:52131408-52131427 None:intergenic 40.0%
TTAGCAAAGTTGCAGCGTTT+AGG + chr7_2:52131409-52131428 None:intergenic 40.0%
GTCCACACTTATAACCAATG+GGG + chr7_2:52131435-52131454 None:intergenic 40.0%
CGTCCACACTTATAACCAAT+GGG + chr7_2:52131436-52131455 None:intergenic 40.0%
ACGTCCACACTTATAACCAA+TGG + chr7_2:52131437-52131456 None:intergenic 40.0%
GGCAACTTAAAAGAAACCGA+GGG + chr7_2:52131475-52131494 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG - chr7_2:52131803-52131822 Msa1061780:intron 40.0%
CAACAACCTCAACTCTTTCA+AGG + chr7_2:52131812-52131831 None:intergenic 40.0%
CAAGAACTGCAATACCAACG+AGG + chr7_2:52131147-52131166 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG - chr7_2:52131151-52131170 Msa1061780:three_prime_UTR 45.0%
! GTTGAAAGTGCAGGTGAATG+TGG - chr7_2:52131184-52131203 Msa1061780:three_prime_UTR 45.0%
AAAGTGCAGGTGAATGTGGA+AGG - chr7_2:52131188-52131207 Msa1061780:three_prime_UTR 45.0%
AAGTGCAGGTGAATGTGGAA+GGG - chr7_2:52131189-52131208 Msa1061780:three_prime_UTR 45.0%
!! AAGGGGTACTACACCAGATA+TGG - chr7_2:52131207-52131226 Msa1061780:three_prime_UTR 45.0%
AACTTTGCTAATCGCCCCAT+TGG - chr7_2:52131418-52131437 Msa1061780:CDS 45.0%
CGGCAACTTAAAAGAAACCG+AGG + chr7_2:52131476-52131495 None:intergenic 45.0%
CAAGAACTGCAATACCAACG+AGG + chr7_2:52131147-52131166 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG - chr7_2:52131151-52131170 Msa1061780:three_prime_UTR 45.0%
! GTTGAAAGTGCAGGTGAATG+TGG - chr7_2:52131184-52131203 Msa1061780:three_prime_UTR 45.0%
AAAGTGCAGGTGAATGTGGA+AGG - chr7_2:52131188-52131207 Msa1061780:three_prime_UTR 45.0%
AAGTGCAGGTGAATGTGGAA+GGG - chr7_2:52131189-52131208 Msa1061780:three_prime_UTR 45.0%
!! AAGGGGTACTACACCAGATA+TGG - chr7_2:52131207-52131226 Msa1061780:three_prime_UTR 45.0%
AACTTTGCTAATCGCCCCAT+TGG - chr7_2:52131418-52131437 Msa1061780:CDS 45.0%
CGGCAACTTAAAAGAAACCG+AGG + chr7_2:52131476-52131495 None:intergenic 45.0%
AGTGCAGGTGAATGTGGAAG+GGG - chr7_2:52131190-52131209 Msa1061780:three_prime_UTR 50.0%
TCTTGTGCTGCACTTGCACA+AGG + chr7_2:52131250-52131269 None:intergenic 50.0%
! CGCCCCATTGGTTATAAGTG+TGG - chr7_2:52131430-52131449 Msa1061780:CDS 50.0%
GTGTACACAAGTGAGCTGCT+AGG + chr7_2:52131948-52131967 None:intergenic 50.0%
AGTGCAGGTGAATGTGGAAG+GGG - chr7_2:52131190-52131209 Msa1061780:three_prime_UTR 50.0%
TCTTGTGCTGCACTTGCACA+AGG + chr7_2:52131250-52131269 None:intergenic 50.0%
! CGCCCCATTGGTTATAAGTG+TGG - chr7_2:52131430-52131449 Msa1061780:CDS 50.0%
GTGTACACAAGTGAGCTGCT+AGG + chr7_2:52131948-52131967 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 52131118 52132148 52131118 ID=Msa1061780;Name=Msa1061780
chr7_2 mRNA 52131118 52132148 52131118 ID=Msa1061780-mRNA-1;Parent=Msa1061780;Name=Msa1061780-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0.5|1|1|1|2|285|114
chr7_2 exon 52131118 52131468 52131118 ID=Msa1061780-mRNA-1:exon:9413;Parent=Msa1061780-mRNA-1
chr7_2 exon 52131870 52132148 52131870 ID=Msa1061780-mRNA-1:exon:9412;Parent=Msa1061780-mRNA-1
chr7_2 CDS 52131870 52132148 52131870 ID=Msa1061780-mRNA-1:cds;Parent=Msa1061780-mRNA-1
chr7_2 CDS 52131403 52131468 52131403 ID=Msa1061780-mRNA-1:cds;Parent=Msa1061780-mRNA-1
chr7_2 three_prime_UTR 52131118 52131402 52131118 ID=Msa1061780-mRNA-1:three_prime_utr;Parent=Msa1061780-mRNA-1
Gene Sequence

>Msa1061780

ATGGAGGGCCTTATGAAATTGTTTTGCCTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCAGGTGAATGTGGAAGGGGTACTACACCAGATATGGAAGCATTTAAGCTTGCTCCTTGTGCAAGTGCAGCACAAGATGAAAATGCTAGTGTCTCACAAAGTTGTTGTGATCAGACTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCAGTTTTGCTCTCAAACGTTGCTAAAATGTCTGGTGTTAACCCTCAAATTGCTCTTACACTCTTCCTTGAAAGAGTTGAGGTTGTTGCTGAAAACAAACATAAATGGGATATGCATTGA

Protein sequence

>Msa1061780

MEGLMKLFCLVGIAVLVAGIHRVESAGECGRGTTPDMEAFKLAPCASAAQDENASVSQSCCDQTKKLGQNPSCLCAVLLSNVAKMSGVNPQIALTLFLERVEVVAENKHKWDMH*