Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006750 | XP_003623166.1 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 1.43e-58 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006750 | G7L5L7 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 6.85e-59 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0036580 | Msa1006750 | 0.804195 | 2.427287e-49 | -8.615850e-47 |
| Msa0061600 | Msa1006750 | 0.810821 | 9.525325e-51 | -8.615850e-47 |
| Msa0645100 | Msa1006750 | 0.819453 | 1.152463e-52 | -8.615850e-47 |
| Msa0645120 | Msa1006750 | 0.822541 | 2.241983e-53 | -8.615850e-47 |
| Msa0684600 | Msa1006750 | 0.805375 | 1.376095e-49 | -8.615850e-47 |
| Msa0139270 | Msa1006750 | 0.807324 | 5.345180e-50 | -8.615850e-47 |
| Msa0234120 | Msa1006750 | 0.806591 | 7.635822e-50 | -8.615850e-47 |
| Msa0424300 | Msa1006750 | 0.804011 | 2.650796e-49 | -8.615850e-47 |
| Msa0469550 | Msa1006750 | 0.801732 | 7.836035e-49 | -8.615850e-47 |
| Msa0471210 | Msa1006750 | 0.802117 | 6.532938e-49 | -8.615850e-47 |
| Msa1006750 | Msa1011320 | 0.802243 | 6.153252e-49 | -8.615850e-47 |
| Msa1006750 | Msa1011350 | 0.814164 | 1.770891e-51 | -8.615850e-47 |
| Msa1006750 | Msa1040730 | 0.803525 | 3.344427e-49 | -8.615850e-47 |
| Msa1006750 | Msa1061760 | 0.836195 | 1.078746e-56 | -8.615850e-47 |
| Msa1006750 | Msa1061780 | 0.800531 | 1.379790e-48 | -8.615850e-47 |
| Msa1006750 | Msa1061800 | 0.801611 | 8.297942e-49 | -8.615850e-47 |
| Msa1006750 | Msa1107310 | 0.820681 | 6.033510e-53 | -8.615850e-47 |
| Msa1006750 | Msa1107320 | 0.849925 | 2.364225e-60 | -8.615850e-47 |
| Msa1006750 | Msa1142860 | 0.814480 | 1.507901e-51 | -8.615850e-47 |
| Msa1006750 | Msa1150860 | 0.859634 | 3.612502e-63 | -8.615850e-47 |
| Msa1006750 | Msa1150870 | 0.816289 | 5.970326e-52 | -8.615850e-47 |
| Msa1006750 | Msa1150880 | 0.905652 | 3.422672e-80 | -8.615850e-47 |
| Msa1006750 | Msa1204160 | 0.825559 | 4.388650e-54 | -8.615850e-47 |
| Msa1006750 | Msa1204190 | 0.830856 | 2.320574e-55 | -8.615850e-47 |
| Msa1006750 | Msa1350490 | 0.803596 | 3.232644e-49 | -8.615850e-47 |
| Msa1006750 | Msa1357490 | 0.804946 | 1.692419e-49 | -8.615850e-47 |
| Msa1006750 | Msa1370930 | 0.808267 | 3.368544e-50 | -8.615850e-47 |
| Msa1006750 | Msa1426420 | 0.802991 | 4.312673e-49 | -8.615850e-47 |
| Msa0267710 | Msa1006750 | 0.837106 | 6.321944e-57 | -8.615850e-47 |
| Msa0548760 | Msa1006750 | 0.824348 | 8.473881e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006750 | MtrunA17_Chr7g0240471 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 1.32e-62 | 186 |
| Msa1006750 | MtrunA17_Chr7g0240451 | 85.417 | 96 | 14 | 0 | 1 | 96 | 1 | 96 | 8.36e-57 | 171 |
| Msa1006750 | MtrunA17_Chr7g0240441 | 67.213 | 61 | 4 | 1 | 36 | 96 | 29 | 73 | 2.32e-19 | 75.9 |
| Msa1006750 | MtrunA17_Chr8g0368861 | 45.946 | 74 | 38 | 2 | 24 | 96 | 27 | 99 | 3.39e-13 | 60.8 |
| Msa1006750 | MtrunA17_Chr8g0368831 | 44.595 | 74 | 39 | 2 | 24 | 96 | 35 | 107 | 1.39e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006750 | AT3G53980.2 | 63.514 | 74 | 26 | 1 | 23 | 96 | 21 | 93 | 1.10e-28 | 100 |
| Msa1006750 | AT3G53980.1 | 63.514 | 74 | 26 | 1 | 23 | 96 | 21 | 93 | 1.10e-28 | 100 |
| Msa1006750 | AT5G05960.1 | 51.613 | 93 | 38 | 2 | 4 | 96 | 10 | 95 | 4.21e-27 | 96.7 |
| Msa1006750 | AT2G37870.1 | 44.186 | 86 | 44 | 3 | 11 | 94 | 9 | 92 | 4.31e-15 | 66.2 |
Find 24 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCATTATC+TGG | 0.246677 | 7_1:-5530672 | None:intergenic |
| TGGATTCTGACCAAGTTTCT+TGG | 0.313730 | 7_1:-5530760 | None:intergenic |
| GTCCTTGTTGCAGGGATTCA+TGG | 0.338655 | 7_1:+5530612 | Msa1006750:CDS |
| CAACAACCTCAACTCTTTCA+AGG | 0.398597 | 7_1:-5531302 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7_1:+5530807 | Msa1006750:CDS |
| TGAAAACAAAGATAATTAAG+TGG | 0.469231 | 7_1:+5531326 | Msa1006750:exon |
| AATAAAACATTCTCTTTCAG+TGG | 0.478071 | 7_1:+5531471 | Msa1006750:three_prime_UTR |
| ATGAAGATGCTGTGCCTAGT+TGG | 0.489536 | 7_1:+5530582 | Msa1006750:CDS |
| GTTGAAAGTGCTGGTGAATG+TGG | 0.534504 | 7_1:+5530636 | Msa1006750:CDS |
| TGTGCTCAGACCAAGAAACT+TGG | 0.540020 | 7_1:+5530750 | Msa1006750:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7_1:-5530699 | None:intergenic |
| CTTTGTTGTTGTTTATAGTG+TGG | 0.544490 | 7_1:+5531412 | Msa1006750:three_prime_UTR |
| AAAGTGCTGGTGAATGTGGA+AGG | 0.549386 | 7_1:+5530640 | Msa1006750:CDS |
| ACTCTTCCTTGAAAGAGTTG+AGG | 0.566469 | 7_1:+5531296 | Msa1006750:CDS |
| GGGATAGTAACAGCAATCTG+AGG | 0.567579 | 7_1:-5530837 | None:intergenic |
| GAAAACAAAGATAATTAAGT+GGG | 0.577679 | 7_1:+5531327 | Msa1006750:exon |
| CAAGGACTACAAAACCAACT+AGG | 0.588924 | 7_1:-5530596 | None:intergenic |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7_1:-5530780 | None:intergenic |
| CTCCATGAATCCCTGCAACA+AGG | 0.594877 | 7_1:-5530614 | None:intergenic |
| ATTCATGGAGTTGAAAGTGC+TGG | 0.616969 | 7_1:+5530627 | Msa1006750:CDS |
| GGATAGTAACAGCAATCTGA+GGG | 0.624763 | 7_1:-5530836 | None:intergenic |
| AATACAACACAACTAAGTTG+TGG | 0.625133 | 7_1:-5531385 | None:intergenic |
| AGTGCTGGTGAATGTGGAAG+GGG | 0.630405 | 7_1:+5530642 | Msa1006750:CDS |
| AAGTGCTGGTGAATGTGGAA+GGG | 0.641454 | 7_1:+5530641 | Msa1006750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGATAGTTAAGAGTATTAT+AGG | + | chr7_1:5531115-5531134 | Msa1006750:intron | 20.0% |
| !! | TGAAAACAAAGATAATTAAG+TGG | + | chr7_1:5531326-5531345 | Msa1006750:exon | 20.0% |
| !! | GAAAACAAAGATAATTAAGT+GGG | + | chr7_1:5531327-5531346 | Msa1006750:exon | 20.0% |
| !!! | TGTCTACTAAAATTTTTTAG+TGG | + | chr7_1:5531503-5531522 | Msa1006750:three_prime_UTR | 20.0% |
| !!! | TTTCAAAAAAAGTGTAGTAA+CGG | - | chr7_1:5531554-5531573 | None:intergenic | 20.0% |
| !!! | TTACTACACTTTTTTTGAAA+AGG | + | chr7_1:5531554-5531573 | Msa1006750:three_prime_UTR | 20.0% |
| !!! | TAGATAGTTAAGAGTATTAT+AGG | + | chr7_1:5531115-5531134 | Msa1006750:intron | 20.0% |
| !! | TGAAAACAAAGATAATTAAG+TGG | + | chr7_1:5531326-5531345 | Msa1006750:exon | 20.0% |
| !! | GAAAACAAAGATAATTAAGT+GGG | + | chr7_1:5531327-5531346 | Msa1006750:exon | 20.0% |
| !!! | TGTCTACTAAAATTTTTTAG+TGG | + | chr7_1:5531503-5531522 | Msa1006750:three_prime_UTR | 20.0% |
| !!! | TTTCAAAAAAAGTGTAGTAA+CGG | - | chr7_1:5531554-5531573 | None:intergenic | 20.0% |
| !!! | TTACTACACTTTTTTTGAAA+AGG | + | chr7_1:5531554-5531573 | Msa1006750:three_prime_UTR | 20.0% |
| ! | TATCTGCAATAGATAGTTAA+AGG | - | chr7_1:5531072-5531091 | None:intergenic | 25.0% |
| ! | ACAACGAAAAAAATGTGAAA+CGG | + | chr7_1:5531163-5531182 | Msa1006750:intron | 25.0% |
| !! | CTATTTGTCAATTTTCTTGA+TGG | + | chr7_1:5531227-5531246 | Msa1006750:intron | 25.0% |
| !!! | AGAGAATGTTTTATTCATGA+GGG | - | chr7_1:5531466-5531485 | None:intergenic | 25.0% |
| !!! | AAGAGAATGTTTTATTCATG+AGG | - | chr7_1:5531467-5531486 | None:intergenic | 25.0% |
| ! | AATAAAACATTCTCTTTCAG+TGG | + | chr7_1:5531471-5531490 | Msa1006750:three_prime_UTR | 25.0% |
| ! | TATCTGCAATAGATAGTTAA+AGG | - | chr7_1:5531072-5531091 | None:intergenic | 25.0% |
| ! | ACAACGAAAAAAATGTGAAA+CGG | + | chr7_1:5531163-5531182 | Msa1006750:intron | 25.0% |
| !! | CTATTTGTCAATTTTCTTGA+TGG | + | chr7_1:5531227-5531246 | Msa1006750:intron | 25.0% |
| !!! | AGAGAATGTTTTATTCATGA+GGG | - | chr7_1:5531466-5531485 | None:intergenic | 25.0% |
| !!! | AAGAGAATGTTTTATTCATG+AGG | - | chr7_1:5531467-5531486 | None:intergenic | 25.0% |
| ! | AATAAAACATTCTCTTTCAG+TGG | + | chr7_1:5531471-5531490 | Msa1006750:three_prime_UTR | 25.0% |
| AGCTTAAATGCTTCATTATC+TGG | - | chr7_1:5530675-5530694 | None:intergenic | 30.0% | |
| GACAACTTAAAAGAAACTGA+GGG | - | chr7_1:5530927-5530946 | None:intergenic | 30.0% | |
| AATACAACACAACTAAGTTG+TGG | - | chr7_1:5531388-5531407 | None:intergenic | 30.0% | |
| !! | CTTTGTTGTTGTTTATAGTG+TGG | + | chr7_1:5531412-5531431 | Msa1006750:three_prime_UTR | 30.0% |
| AGCTTAAATGCTTCATTATC+TGG | - | chr7_1:5530675-5530694 | None:intergenic | 30.0% | |
| GACAACTTAAAAGAAACTGA+GGG | - | chr7_1:5530927-5530946 | None:intergenic | 30.0% | |
| AATACAACACAACTAAGTTG+TGG | - | chr7_1:5531388-5531407 | None:intergenic | 30.0% | |
| !! | CTTTGTTGTTGTTTATAGTG+TGG | + | chr7_1:5531412-5531431 | Msa1006750:three_prime_UTR | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | - | chr7_1:5530783-5530802 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7_1:5530807-5530826 | Msa1006750:CDS | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | - | chr7_1:5530928-5530947 | None:intergenic | 35.0% | |
| AAAACTGCACAAAGACAACT+TGG | - | chr7_1:5530783-5530802 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7_1:5530807-5530826 | Msa1006750:CDS | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | - | chr7_1:5530928-5530947 | None:intergenic | 35.0% | |
| CAAGGACTACAAAACCAACT+AGG | - | chr7_1:5530599-5530618 | None:intergenic | 40.0% | |
| ! | GTTTTGTAGTCCTTGTTGCA+GGG | + | chr7_1:5530604-5530623 | Msa1006750:CDS | 40.0% |
| ! | ATTCATGGAGTTGAAAGTGC+TGG | + | chr7_1:5530627-5530646 | Msa1006750:CDS | 40.0% |
| TGGATTCTGACCAAGTTTCT+TGG | - | chr7_1:5530763-5530782 | None:intergenic | 40.0% | |
| GGATAGTAACAGCAATCTGA+GGG | - | chr7_1:5530839-5530858 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7_1:5530860-5530879 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7_1:5530861-5530880 | None:intergenic | 40.0% | |
| AACTTTGCTAATCGTCCCAT+TGG | + | chr7_1:5530870-5530889 | Msa1006750:intron | 40.0% | |
| ACTCTTCCTTGAAAGAGTTG+AGG | + | chr7_1:5531296-5531315 | Msa1006750:CDS | 40.0% | |
| CAACAACCTCAACTCTTTCA+AGG | - | chr7_1:5531305-5531324 | None:intergenic | 40.0% | |
| CAAGGACTACAAAACCAACT+AGG | - | chr7_1:5530599-5530618 | None:intergenic | 40.0% | |
| ! | GTTTTGTAGTCCTTGTTGCA+GGG | + | chr7_1:5530604-5530623 | Msa1006750:CDS | 40.0% |
| ! | ATTCATGGAGTTGAAAGTGC+TGG | + | chr7_1:5530627-5530646 | Msa1006750:CDS | 40.0% |
| TGGATTCTGACCAAGTTTCT+TGG | - | chr7_1:5530763-5530782 | None:intergenic | 40.0% | |
| GGATAGTAACAGCAATCTGA+GGG | - | chr7_1:5530839-5530858 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7_1:5530860-5530879 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7_1:5530861-5530880 | None:intergenic | 40.0% | |
| AACTTTGCTAATCGTCCCAT+TGG | + | chr7_1:5530870-5530889 | Msa1006750:intron | 40.0% | |
| ACTCTTCCTTGAAAGAGTTG+AGG | + | chr7_1:5531296-5531315 | Msa1006750:CDS | 40.0% | |
| CAACAACCTCAACTCTTTCA+AGG | - | chr7_1:5531305-5531324 | None:intergenic | 40.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | + | chr7_1:5530582-5530601 | Msa1006750:CDS | 45.0% | |
| !! | GGTTTTGTAGTCCTTGTTGC+AGG | + | chr7_1:5530603-5530622 | Msa1006750:CDS | 45.0% |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | + | chr7_1:5530636-5530655 | Msa1006750:CDS | 45.0% |
| !! | AAAGTGCTGGTGAATGTGGA+AGG | + | chr7_1:5530640-5530659 | Msa1006750:CDS | 45.0% |
| !! | AAGTGCTGGTGAATGTGGAA+GGG | + | chr7_1:5530641-5530660 | Msa1006750:CDS | 45.0% |
| TGTGCTCAGACCAAGAAACT+TGG | + | chr7_1:5530750-5530769 | Msa1006750:CDS | 45.0% | |
| GGGATAGTAACAGCAATCTG+AGG | - | chr7_1:5530840-5530859 | None:intergenic | 45.0% | |
| ! | CGTCCCATTGGTTATAAGTG+CGG | + | chr7_1:5530882-5530901 | Msa1006750:intron | 45.0% |
| CGTCCGCACTTATAACCAAT+GGG | - | chr7_1:5530888-5530907 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | - | chr7_1:5530889-5530908 | None:intergenic | 45.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | + | chr7_1:5530582-5530601 | Msa1006750:CDS | 45.0% | |
| !! | GGTTTTGTAGTCCTTGTTGC+AGG | + | chr7_1:5530603-5530622 | Msa1006750:CDS | 45.0% |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | + | chr7_1:5530636-5530655 | Msa1006750:CDS | 45.0% |
| !! | AAAGTGCTGGTGAATGTGGA+AGG | + | chr7_1:5530640-5530659 | Msa1006750:CDS | 45.0% |
| !! | AAGTGCTGGTGAATGTGGAA+GGG | + | chr7_1:5530641-5530660 | Msa1006750:CDS | 45.0% |
| TGTGCTCAGACCAAGAAACT+TGG | + | chr7_1:5530750-5530769 | Msa1006750:CDS | 45.0% | |
| GGGATAGTAACAGCAATCTG+AGG | - | chr7_1:5530840-5530859 | None:intergenic | 45.0% | |
| ! | CGTCCCATTGGTTATAAGTG+CGG | + | chr7_1:5530882-5530901 | Msa1006750:intron | 45.0% |
| CGTCCGCACTTATAACCAAT+GGG | - | chr7_1:5530888-5530907 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | - | chr7_1:5530889-5530908 | None:intergenic | 45.0% | |
| GTCCTTGTTGCAGGGATTCA+TGG | + | chr7_1:5530612-5530631 | Msa1006750:CDS | 50.0% | |
| CTCCATGAATCCCTGCAACA+AGG | - | chr7_1:5530617-5530636 | None:intergenic | 50.0% | |
| !! | AGTGCTGGTGAATGTGGAAG+GGG | + | chr7_1:5530642-5530661 | Msa1006750:CDS | 50.0% |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7_1:5530702-5530721 | None:intergenic | 50.0% | |
| GTCCTTGTTGCAGGGATTCA+TGG | + | chr7_1:5530612-5530631 | Msa1006750:CDS | 50.0% | |
| CTCCATGAATCCCTGCAACA+AGG | - | chr7_1:5530617-5530636 | None:intergenic | 50.0% | |
| !! | AGTGCTGGTGAATGTGGAAG+GGG | + | chr7_1:5530642-5530661 | Msa1006750:CDS | 50.0% |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7_1:5530702-5530721 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 5530570 | 5531594 | 5530570 | ID=Msa1006750;Name=Msa1006750 |
| chr7_1 | mRNA | 5530570 | 5531594 | 5530570 | ID=Msa1006750-mRNA-1;Parent=Msa1006750;Name=Msa1006750-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|1|1|1|2|250|110 |
| chr7_1 | exon | 5530570 | 5530848 | 5530570 | ID=Msa1006750-mRNA-1:exon:1795;Parent=Msa1006750-mRNA-1 |
| chr7_1 | exon | 5531291 | 5531594 | 5531291 | ID=Msa1006750-mRNA-1:exon:1796;Parent=Msa1006750-mRNA-1 |
| chr7_1 | CDS | 5530570 | 5530848 | 5530570 | ID=Msa1006750-mRNA-1:cds;Parent=Msa1006750-mRNA-1 |
| chr7_1 | CDS | 5531291 | 5531344 | 5531291 | ID=Msa1006750-mRNA-1:cds;Parent=Msa1006750-mRNA-1 |
| chr7_1 | three_prime_UTR | 5531345 | 5531594 | 5531345 | ID=Msa1006750-mRNA-1:three_prime_utr;Parent=Msa1006750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
