AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1150860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1150860 MtrunA17_Chr7g0240451 95.726 117 5 0 1 117 1 117 6.12e-80 230
Msa1150860 MtrunA17_Chr7g0240471 87.179 117 15 0 1 117 1 117 5.33e-73 213
Msa1150860 MtrunA17_Chr7g0240441 74.026 77 4 1 36 112 29 89 1.27e-33 112
Msa1150860 MtrunA17_Chr8g0368861 45.455 121 61 3 1 117 1 120 3.05e-25 92.0
Msa1150860 MtrunA17_Chr8g0368831 44.628 121 62 3 1 117 9 128 1.43e-24 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1150860 AT5G05960.1 61.261 111 36 2 7 117 13 116 1.10e-42 136
Msa1150860 AT3G53980.2 64.211 95 33 1 23 117 21 114 2.97e-42 135
Msa1150860 AT3G53980.1 64.211 95 33 1 23 117 21 114 2.97e-42 135
Msa1150860 AT2G37870.1 49.107 112 51 4 10 117 6 115 5.51e-26 94.0

Find 31 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCTGTTAGCTGCTATTA+TGG 0.326649 7_4:-34696363 Msa1150860:three_prime_UTR
GGTATTGCAGTTCTTGTTGC+AGG 0.361788 7_4:-34697053 Msa1150860:CDS
GGGTTACTATTTAATATAGT+TGG 0.381173 7_4:-34696174 Msa1150860:three_prime_UTR
CAACAACCTCAACTCTTTCA+AGG 0.398597 7_4:+34696416 None:intergenic
AGCTTAAAAGCTTCATTGTC+TGG 0.411064 7_4:+34696984 None:intergenic
ACTATTTAATATAGTTGGTA+TGG 0.424897 7_4:-34696169 Msa1150860:three_prime_UTR
TCAAACGTTGCTAAAATGTC+TGG 0.431901 7_4:-34696849 Msa1150860:CDS
CAGCAAGGTTGCAACGCTTA+GGG 0.465106 7_4:+34696799 None:intergenic
GGATAGTAACAGCAATTTGA+GGG 0.472152 7_4:+34696820 None:intergenic
TTGCTGAAAACAAAGATAAG+AGG 0.488652 7_4:-34696396 Msa1150860:three_prime_UTR
TCAGCAAGGTTGCAACGCTT+AGG 0.488806 7_4:+34696798 None:intergenic
GTGAAATTGTTGTGCTTCGT+TGG 0.500117 7_4:-34697074 Msa1150860:CDS
GGGATAGTAACAGCAATTTG+AGG 0.508534 7_4:+34696819 None:intergenic
GTCACAAAATTGTTGTGCTC+AGG 0.514410 7_4:-34696919 Msa1150860:CDS
CGTCCACACTTGTAACCGAC+AGG 0.520932 7_4:+34696771 None:intergenic
CGCCCTGTCGGTTACAAGTG+TGG 0.534054 7_4:-34696774 Msa1150860:CDS
GTTGAAAGTGCTGGTGAATG+TGG 0.539242 7_4:-34697020 Msa1150860:CDS
TAACTTTACTGTTTATAGTG+TGG 0.540728 7_4:-34696313 Msa1150860:three_prime_UTR
AAAGTGCTGGTGAATGTGGA+AGG 0.549386 7_4:-34697016 Msa1150860:CDS
AACCTTGCTGATCGCCCTGT+CGG 0.551684 7_4:-34696786 Msa1150860:CDS
ATTCATAGAGTTGAAAGTGC+TGG 0.554882 7_4:-34697029 Msa1150860:CDS
ACTCTTCCTTGAAAGAGTTG+AGG 0.566469 7_4:-34696422 Msa1150860:exon
TGCTGAAAACAAAGATAAGA+GGG 0.575968 7_4:-34696395 Msa1150860:three_prime_UTR
ATAACAGCACAAAGACAACT+TGG 0.604458 7_4:+34696876 None:intergenic
TGTGCTCAGGTTAAGAAACT+CGG 0.610168 7_4:-34696906 Msa1150860:CDS
TTTATTCAGGGTGTTCACAA+AGG 0.622018 7_4:+34696274 None:intergenic
AGTGCTGGTGAATGTGGAAG+GGG 0.630405 7_4:-34697014 Msa1150860:CDS
AACCGACAGGGCGATCAGCA+AGG 0.631783 7_4:+34696784 None:intergenic
AAGTGCTGGTGAATGTGGAA+GGG 0.641454 7_4:-34697015 Msa1150860:CDS
AATACAACACAACTAAGCTG+TGG 0.678775 7_4:+34696337 None:intergenic
GTCCACACTTGTAACCGACA+GGG 0.709589 7_4:+34696772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTAAGAAGAAAAAAAA+TGG - chr7_4:34696188-34696207 Msa1150860:three_prime_UTR 10.0%
!!! TAATTATTTATCTTTTTTCT+TGG - chr7_4:34696795-34696814 Msa1150860:CDS 10.0%
!! TATATTAAGAAGAAAAAAAA+TGG - chr7_4:34696188-34696207 Msa1150860:three_prime_UTR 10.0%
!!! TAATTATTTATCTTTTTTCT+TGG - chr7_4:34696795-34696814 Msa1150860:CDS 10.0%
!!! TACTTATTTGACATTTTTTT+AGG + chr7_4:34696757-34696776 None:intergenic 15.0%
!!! TACTTATTTGACATTTTTTT+AGG + chr7_4:34696757-34696776 None:intergenic 15.0%
!! ATTAAGAAGAAAAAAAATGG+AGG - chr7_4:34696191-34696210 Msa1150860:three_prime_UTR 20.0%
!! TTAAGAAGAAAAAAAATGGA+GGG - chr7_4:34696192-34696211 Msa1150860:three_prime_UTR 20.0%
!!! AAATGAAACTGTTTTATTCA+GGG + chr7_4:34697034-34697053 None:intergenic 20.0%
!!! AAAATGAAACTGTTTTATTC+AGG + chr7_4:34697035-34697054 None:intergenic 20.0%
!!! GATGCTTTTACATTATATTT+GGG - chr7_4:34697094-34697113 Msa1150860:exon 20.0%
!!! TTTTACATTATATTTGGGTT+GGG - chr7_4:34697099-34697118 Msa1150860:exon 20.0%
!!! ACTATTTAATATAGTTGGTA+TGG - chr7_4:34697124-34697143 Msa1150860:five_prime_UTR 20.0%
!! ATTAAGAAGAAAAAAAATGG+AGG - chr7_4:34696191-34696210 Msa1150860:three_prime_UTR 20.0%
!! TTAAGAAGAAAAAAAATGGA+GGG - chr7_4:34696192-34696211 Msa1150860:three_prime_UTR 20.0%
!!! AAATGAAACTGTTTTATTCA+GGG + chr7_4:34697034-34697053 None:intergenic 20.0%
!!! AAAATGAAACTGTTTTATTC+AGG + chr7_4:34697035-34697054 None:intergenic 20.0%
!!! GATGCTTTTACATTATATTT+GGG - chr7_4:34697094-34697113 Msa1150860:exon 20.0%
!!! TTTTACATTATATTTGGGTT+GGG - chr7_4:34697099-34697118 Msa1150860:exon 20.0%
!!! ACTATTTAATATAGTTGGTA+TGG - chr7_4:34697124-34697143 Msa1150860:five_prime_UTR 20.0%
! TAACTTTACTGTTTATAGTG+TGG - chr7_4:34696980-34696999 Msa1150860:CDS 25.0%
!! GGATGCTTTTACATTATATT+TGG - chr7_4:34697093-34697112 Msa1150860:exon 25.0%
!! CTTTTACATTATATTTGGGT+TGG - chr7_4:34697098-34697117 Msa1150860:exon 25.0%
! GGGTTACTATTTAATATAGT+TGG - chr7_4:34697119-34697138 Msa1150860:five_prime_UTR 25.0%
! TAACTTTACTGTTTATAGTG+TGG - chr7_4:34696980-34696999 Msa1150860:CDS 25.0%
!! GGATGCTTTTACATTATATT+TGG - chr7_4:34697093-34697112 Msa1150860:exon 25.0%
!! CTTTTACATTATATTTGGGT+TGG - chr7_4:34697098-34697117 Msa1150860:exon 25.0%
! GGGTTACTATTTAATATAGT+TGG - chr7_4:34697119-34697138 Msa1150860:five_prime_UTR 25.0%
TTGCTGAAAACAAAGATAAG+AGG - chr7_4:34696897-34696916 Msa1150860:CDS 30.0%
TGCTGAAAACAAAGATAAGA+GGG - chr7_4:34696898-34696917 Msa1150860:CDS 30.0%
!!! ATCCACTAAAAAGTTTTGGA+AGG + chr7_4:34697077-34697096 None:intergenic 30.0%
! AAGCATCCACTAAAAAGTTT+TGG + chr7_4:34697081-34697100 None:intergenic 30.0%
TTGCTGAAAACAAAGATAAG+AGG - chr7_4:34696897-34696916 Msa1150860:CDS 30.0%
TGCTGAAAACAAAGATAAGA+GGG - chr7_4:34696898-34696917 Msa1150860:CDS 30.0%
!!! ATCCACTAAAAAGTTTTGGA+AGG + chr7_4:34697077-34697096 None:intergenic 30.0%
! AAGCATCCACTAAAAAGTTT+TGG + chr7_4:34697081-34697100 None:intergenic 30.0%
! ATTCATAGAGTTGAAAGTGC+TGG - chr7_4:34696264-34696283 Msa1150860:three_prime_UTR 35.0%
AGCTTAAAAGCTTCATTGTC+TGG + chr7_4:34696312-34696331 None:intergenic 35.0%
ATAACAGCACAAAGACAACT+TGG + chr7_4:34696420-34696439 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG - chr7_4:34696444-34696463 Msa1150860:intron 35.0%
GGATAGTAACAGCAATTTGA+GGG + chr7_4:34696476-34696495 None:intergenic 35.0%
AATACAACACAACTAAGCTG+TGG + chr7_4:34696959-34696978 None:intergenic 35.0%
! TTTATTCAGGGTGTTCACAA+AGG + chr7_4:34697022-34697041 None:intergenic 35.0%
! TGCCTTCCAAAACTTTTTAG+TGG - chr7_4:34697072-34697091 Msa1150860:CDS 35.0%
! ATTCATAGAGTTGAAAGTGC+TGG - chr7_4:34696264-34696283 Msa1150860:three_prime_UTR 35.0%
AGCTTAAAAGCTTCATTGTC+TGG + chr7_4:34696312-34696331 None:intergenic 35.0%
ATAACAGCACAAAGACAACT+TGG + chr7_4:34696420-34696439 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG - chr7_4:34696444-34696463 Msa1150860:intron 35.0%
GGATAGTAACAGCAATTTGA+GGG + chr7_4:34696476-34696495 None:intergenic 35.0%
AATACAACACAACTAAGCTG+TGG + chr7_4:34696959-34696978 None:intergenic 35.0%
! TTTATTCAGGGTGTTCACAA+AGG + chr7_4:34697022-34697041 None:intergenic 35.0%
! TGCCTTCCAAAACTTTTTAG+TGG - chr7_4:34697072-34697091 Msa1150860:CDS 35.0%
GTGAAATTGTTGTGCTTCGT+TGG - chr7_4:34696219-34696238 Msa1150860:three_prime_UTR 40.0%
GTCACAAAATTGTTGTGCTC+AGG - chr7_4:34696374-34696393 Msa1150860:three_prime_UTR 40.0%
TGTGCTCAGGTTAAGAAACT+CGG - chr7_4:34696387-34696406 Msa1150860:three_prime_UTR 40.0%
GGGATAGTAACAGCAATTTG+AGG + chr7_4:34696477-34696496 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG - chr7_4:34696871-34696890 Msa1150860:CDS 40.0%
CAACAACCTCAACTCTTTCA+AGG + chr7_4:34696880-34696899 None:intergenic 40.0%
! GTTGCTGTTAGCTGCTATTA+TGG - chr7_4:34696930-34696949 Msa1150860:CDS 40.0%
GTGAAATTGTTGTGCTTCGT+TGG - chr7_4:34696219-34696238 Msa1150860:three_prime_UTR 40.0%
GTCACAAAATTGTTGTGCTC+AGG - chr7_4:34696374-34696393 Msa1150860:three_prime_UTR 40.0%
TGTGCTCAGGTTAAGAAACT+CGG - chr7_4:34696387-34696406 Msa1150860:three_prime_UTR 40.0%
GGGATAGTAACAGCAATTTG+AGG + chr7_4:34696477-34696496 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG - chr7_4:34696871-34696890 Msa1150860:CDS 40.0%
CAACAACCTCAACTCTTTCA+AGG + chr7_4:34696880-34696899 None:intergenic 40.0%
! GTTGCTGTTAGCTGCTATTA+TGG - chr7_4:34696930-34696949 Msa1150860:CDS 40.0%
GGTATTGCAGTTCTTGTTGC+AGG - chr7_4:34696240-34696259 Msa1150860:three_prime_UTR 45.0%
!! GTTGAAAGTGCTGGTGAATG+TGG - chr7_4:34696273-34696292 Msa1150860:three_prime_UTR 45.0%
!! AAAGTGCTGGTGAATGTGGA+AGG - chr7_4:34696277-34696296 Msa1150860:three_prime_UTR 45.0%
!! AAGTGCTGGTGAATGTGGAA+GGG - chr7_4:34696278-34696297 Msa1150860:three_prime_UTR 45.0%
! TCTTTTGCTGCACTTGCACA+AGG + chr7_4:34696339-34696358 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG - chr7_4:34696240-34696259 Msa1150860:three_prime_UTR 45.0%
!! GTTGAAAGTGCTGGTGAATG+TGG - chr7_4:34696273-34696292 Msa1150860:three_prime_UTR 45.0%
!! AAAGTGCTGGTGAATGTGGA+AGG - chr7_4:34696277-34696296 Msa1150860:three_prime_UTR 45.0%
!! AAGTGCTGGTGAATGTGGAA+GGG - chr7_4:34696278-34696297 Msa1150860:three_prime_UTR 45.0%
! TCTTTTGCTGCACTTGCACA+AGG + chr7_4:34696339-34696358 None:intergenic 45.0%
!! AGTGCTGGTGAATGTGGAAG+GGG - chr7_4:34696279-34696298 Msa1150860:three_prime_UTR 50.0%
CAGCAAGGTTGCAACGCTTA+GGG + chr7_4:34696497-34696516 None:intergenic 50.0%
TCAGCAAGGTTGCAACGCTT+AGG + chr7_4:34696498-34696517 None:intergenic 50.0%
GTCCACACTTGTAACCGACA+GGG + chr7_4:34696524-34696543 None:intergenic 50.0%
!! AGTGCTGGTGAATGTGGAAG+GGG - chr7_4:34696279-34696298 Msa1150860:three_prime_UTR 50.0%
CAGCAAGGTTGCAACGCTTA+GGG + chr7_4:34696497-34696516 None:intergenic 50.0%
TCAGCAAGGTTGCAACGCTT+AGG + chr7_4:34696498-34696517 None:intergenic 50.0%
GTCCACACTTGTAACCGACA+GGG + chr7_4:34696524-34696543 None:intergenic 50.0%
AACCTTGCTGATCGCCCTGT+CGG - chr7_4:34696507-34696526 Msa1150860:intron 55.0%
CGTCCACACTTGTAACCGAC+AGG + chr7_4:34696525-34696544 None:intergenic 55.0%
AACCTTGCTGATCGCCCTGT+CGG - chr7_4:34696507-34696526 Msa1150860:intron 55.0%
CGTCCACACTTGTAACCGAC+AGG + chr7_4:34696525-34696544 None:intergenic 55.0%
AACCGACAGGGCGATCAGCA+AGG + chr7_4:34696512-34696531 None:intergenic 60.0%
! CGCCCTGTCGGTTACAAGTG+TGG - chr7_4:34696519-34696538 Msa1150860:intron 60.0%
AACCGACAGGGCGATCAGCA+AGG + chr7_4:34696512-34696531 None:intergenic 60.0%
! CGCCCTGTCGGTTACAAGTG+TGG - chr7_4:34696519-34696538 Msa1150860:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 34696165 34697150 34696165 ID=Msa1150860;Name=Msa1150860
chr7_4 mRNA 34696165 34697150 34696165 ID=Msa1150860-mRNA-1;Parent=Msa1150860;Name=Msa1150860-mRNA-1;_AED=0.26;_eAED=0.26;_QI=42|1|1|1|1|1|2|268|117
chr7_4 exon 34696165 34696449 34696165 ID=Msa1150860-mRNA-1:exon:8243;Parent=Msa1150860-mRNA-1
chr7_4 exon 34696772 34697150 34696772 ID=Msa1150860-mRNA-1:exon:8242;Parent=Msa1150860-mRNA-1
chr7_4 five_prime_UTR 34697109 34697150 34697109 ID=Msa1150860-mRNA-1:five_prime_utr;Parent=Msa1150860-mRNA-1
chr7_4 CDS 34696772 34697108 34696772 ID=Msa1150860-mRNA-1:cds;Parent=Msa1150860-mRNA-1
chr7_4 CDS 34696433 34696449 34696433 ID=Msa1150860-mRNA-1:cds;Parent=Msa1150860-mRNA-1
chr7_4 three_prime_UTR 34696165 34696432 34696165 ID=Msa1150860-mRNA-1:three_prime_utr;Parent=Msa1150860-mRNA-1
Gene Sequence

>Msa1150860

ATGGAGGGTCTTGTGAAATTGTTGTGCTTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCTGGTGAATGTGGAAGGGGTACTACACCAGACAATGAAGCTTTTAAGCTACTTCCTTGTGCAAGTGCAGCAAAAGATGAAAATGCTAGTGTGTCACAAAATTGTTGTGCTCAGGTTAAGAAACTCGGTCAGAATCCAAGTTGTCTTTGTGCTGTTATGCTTTCAAACGTTGCTAAAATGTCTGGTGCTAACCCTCAAATTGCTGTTACTATCCCTAAGCGTTGCAACCTTGCTGATCGCCCTGTCGGTTACAAGTGTGGACCTTACACTCTTCCTTGA

Protein sequence

>Msa1150860

MEGLVKLLCFVGIAVLVAGIHRVESAGECGRGTTPDNEAFKLLPCASAAKDENASVSQNCCAQVKKLGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCGPYTLP*