Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1267930 | XP_003629785.1 | 99.427 | 524 | 3 | 0 | 1 | 524 | 1 | 524 | 0.0 | 1059 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1267930 | sp|Q94C70|NAT2_ARATH | 83.588 | 524 | 86 | 0 | 1 | 524 | 1 | 524 | 0.0 | 926 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003080 | Msa1267930 | 0.804313 | 2.293192e-49 | -8.615850e-47 |
| Msa0005020 | Msa1267930 | 0.802365 | 5.808959e-49 | -8.615850e-47 |
| Msa0006840 | Msa1267930 | 0.884093 | 2.574243e-71 | -8.615850e-47 |
| Msa0009780 | Msa1267930 | 0.831822 | 1.342409e-55 | -8.615850e-47 |
| Msa0015150 | Msa1267930 | 0.812293 | 4.559466e-51 | -8.615850e-47 |
| Msa0015570 | Msa1267930 | 0.885186 | 1.010336e-71 | -8.615850e-47 |
| Msa0016530 | Msa1267930 | 0.851465 | 8.718945e-61 | -8.615850e-47 |
| Msa0018920 | Msa1267930 | 0.877592 | 5.553105e-69 | -8.615850e-47 |
| Msa0019930 | Msa1267930 | 0.816378 | 5.702314e-52 | -8.615850e-47 |
| Msa0020540 | Msa1267930 | 0.845917 | 3.011502e-59 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1267930 | MtrunA17_Chr8g0379171 | 99.427 | 524 | 3 | 0 | 1 | 524 | 1 | 524 | 0.0 | 1059 |
| Msa1267930 | MtrunA17_Chr2g0284061 | 83.397 | 524 | 87 | 0 | 1 | 524 | 1 | 524 | 0.0 | 916 |
| Msa1267930 | MtrunA17_Chr3g0119521 | 72.587 | 518 | 141 | 1 | 8 | 524 | 3 | 520 | 0.0 | 793 |
| Msa1267930 | MtrunA17_Chr8g0364611 | 59.846 | 518 | 206 | 1 | 7 | 522 | 14 | 531 | 0.0 | 645 |
| Msa1267930 | MtrunA17_Chr3g0117941 | 59.653 | 518 | 207 | 1 | 7 | 522 | 13 | 530 | 0.0 | 633 |
| Msa1267930 | MtrunA17_Chr4g0059331 | 59.266 | 518 | 209 | 1 | 7 | 522 | 13 | 530 | 0.0 | 629 |
| Msa1267930 | MtrunA17_Chr1g0154001 | 57.534 | 511 | 217 | 0 | 12 | 522 | 35 | 545 | 0.0 | 607 |
| Msa1267930 | MtrunA17_Chr5g0414131 | 56.298 | 524 | 221 | 4 | 5 | 522 | 15 | 536 | 0.0 | 597 |
| Msa1267930 | MtrunA17_Chr1g0207021 | 54.356 | 528 | 232 | 4 | 1 | 522 | 1 | 525 | 0.0 | 572 |
| Msa1267930 | MtrunA17_Chr3g0134911 | 48.998 | 549 | 240 | 5 | 9 | 522 | 40 | 583 | 0.0 | 550 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1267930 | AT2G34190.1 | 83.588 | 524 | 86 | 0 | 1 | 524 | 1 | 524 | 0.0 | 926 |
| Msa1267930 | AT2G05760.1 | 70.077 | 518 | 154 | 1 | 8 | 524 | 3 | 520 | 0.0 | 764 |
| Msa1267930 | AT5G62890.1 | 59.266 | 518 | 209 | 1 | 7 | 522 | 13 | 530 | 0.0 | 628 |
| Msa1267930 | AT5G62890.2 | 59.266 | 518 | 209 | 1 | 7 | 522 | 13 | 530 | 0.0 | 628 |
| Msa1267930 | AT5G62890.3 | 59.266 | 518 | 209 | 1 | 7 | 522 | 13 | 530 | 0.0 | 628 |
| Msa1267930 | AT1G60030.1 | 57.198 | 514 | 218 | 1 | 11 | 522 | 23 | 536 | 0.0 | 621 |
| Msa1267930 | AT2G26510.1 | 57.553 | 523 | 221 | 1 | 1 | 522 | 25 | 547 | 0.0 | 612 |
| Msa1267930 | AT1G10540.1 | 54.771 | 524 | 231 | 4 | 5 | 522 | 14 | 537 | 0.0 | 590 |
| Msa1267930 | AT5G49990.2 | 56.371 | 518 | 224 | 1 | 7 | 522 | 9 | 526 | 0.0 | 586 |
| Msa1267930 | AT5G49990.3 | 56.371 | 518 | 224 | 1 | 7 | 522 | 9 | 526 | 0.0 | 586 |
Find 150 sgRNAs with CRISPR-Local
Find 276 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGGGTCACGAATAA+TGG | 0.387784 | 8_3:-273057 | None:intergenic |
| AAACCAACTAATGTAATTAC+TGG | 0.315074 | 8_3:-273514 | None:intergenic |
| AAACCCTGAAGTTGATCCAT+TGG | 0.502837 | 8_3:-271719 | None:intergenic |
| AAACTAAGTGTCACAGTAGT+GGG | 0.565633 | 8_3:+271532 | Msa1267930:five_prime_UTR |
| AAAGGTTCGATATATGTTTA+TGG | 0.205413 | 8_3:+275923 | Msa1267930:three_prime_UTR |
| AAATATGGGCTATTTGTTCT+AGG | 0.325670 | 8_3:+273333 | Msa1267930:CDS |
| AACATAGTTGTAGGACAGAC+AGG | 0.467914 | 8_3:+274114 | Msa1267930:CDS |
| AACCGGTTAGTGTTCCGAAA+AGG | 0.514137 | 8_3:-274587 | None:intergenic |
| AACCTTTCCAATGCAGCAAT+TGG | 0.318940 | 8_3:-275907 | None:intergenic |
| AAGAAGCGGTTGAGATTGAA+AGG | 0.503438 | 8_3:-275660 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATAGATTCAAATCCATCT+TGG | + | chr8_3:271750-271769 | Msa1267930:CDS | 30.0% | |
| ATAGATTCAAATCCATCTTG+GGG | + | chr8_3:271752-271771 | Msa1267930:CDS | 30.0% | |
| ATTGAAGGAACAAGAAATGA+AGG | - | chr8_3:271938-271957 | None:intergenic | 30.0% | |
| TTCATTTCTTGTTCCTTCAA+TGG | + | chr8_3:271937-271956 | Msa1267930:CDS | 30.0% | |
| TCATTTCTTGTTCCTTCAAT+GGG | + | chr8_3:271938-271957 | Msa1267930:CDS | 30.0% | |
| AACTGGTTGAATTGGAAATT+GGG | + | chr8_3:272169-272188 | Msa1267930:intron | 30.0% | |
| GTATGTTTAACAGGTTGTAA+CGG | + | chr8_3:272192-272211 | Msa1267930:intron | 30.0% | |
| AAACCAATAGACAAACTAGT+AGG | - | chr8_3:272221-272240 | None:intergenic | 30.0% | |
| AGTTGAATCAGATTGAATAG+TGG | + | chr8_3:272263-272282 | Msa1267930:intron | 30.0% | |
| GACAATGAAAAATACTAGCA+AGG | + | chr8_3:272494-272513 | Msa1267930:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 271478 | 275978 | 271478 | ID=Msa1267930;Name=Msa1267930 |
| chr8_3 | mRNA | 271478 | 275978 | 271478 | ID=Msa1267930-mRNA-1;Parent=Msa1267930;Name=Msa1267930-mRNA-1;_AED=0.17;_eAED=0.17;_QI=205|1|1|1|1|1|13|283|524 |
| chr8_3 | exon | 271478 | 271773 | 271478 | ID=Msa1267930-mRNA-1:exon:32;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 271873 | 271970 | 271873 | ID=Msa1267930-mRNA-1:exon:33;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 272927 | 273103 | 272927 | ID=Msa1267930-mRNA-1:exon:34;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 273254 | 273354 | 273254 | ID=Msa1267930-mRNA-1:exon:35;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 273489 | 273564 | 273489 | ID=Msa1267930-mRNA-1:exon:36;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 273672 | 273731 | 273672 | ID=Msa1267930-mRNA-1:exon:37;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 273978 | 274162 | 273978 | ID=Msa1267930-mRNA-1:exon:38;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 274263 | 274359 | 274263 | ID=Msa1267930-mRNA-1:exon:39;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 274477 | 274622 | 274477 | ID=Msa1267930-mRNA-1:exon:40;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 274705 | 274796 | 274705 | ID=Msa1267930-mRNA-1:exon:41;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 274906 | 274983 | 274906 | ID=Msa1267930-mRNA-1:exon:42;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 275080 | 275240 | 275080 | ID=Msa1267930-mRNA-1:exon:43;Parent=Msa1267930-mRNA-1 |
| chr8_3 | exon | 275483 | 275978 | 275483 | ID=Msa1267930-mRNA-1:exon:44;Parent=Msa1267930-mRNA-1 |
| chr8_3 | five_prime_UTR | 271478 | 271682 | 271478 | ID=Msa1267930-mRNA-1:five_prime_utr;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 271683 | 271773 | 271683 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 271873 | 271970 | 271873 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 272927 | 273103 | 272927 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 273254 | 273354 | 273254 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 273489 | 273564 | 273489 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 273672 | 273731 | 273672 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 273978 | 274162 | 273978 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 274263 | 274359 | 274263 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 274477 | 274622 | 274477 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 274705 | 274796 | 274705 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 274906 | 274983 | 274906 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 275080 | 275240 | 275080 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | CDS | 275483 | 275695 | 275483 | ID=Msa1267930-mRNA-1:cds;Parent=Msa1267930-mRNA-1 |
| chr8_3 | three_prime_UTR | 275696 | 275978 | 275696 | ID=Msa1267930-mRNA-1:three_prime_utr;Parent=Msa1267930-mRNA-1 |
| Gene Sequence |
>Msa1267930 ATGGCAGCTATAAAGCCAGAAGAGATAAGTCATTCACCAATGGATCAACTTCAGGGTTTAGAGTATTGTATAGATTCAAATCCATCTTGGGTGGAGACAATACTTCTTGGTTTTCAACATTATATTTTGGCATTAGGAACTGCAGTTATGATTCCTTCATTTCTTGTTCCTTCAATGGGTGGAAATGATGATGATAAAGTAAGGGTGGTACAAACTCTACTTTTTGTTGAAGGAATTAATACACTTTTGCAAACACTGTTTGGAACCCGGTTACCAACAGTGATAGGAGGATCATATGCATTCATGGTCCCCATTGTATCCATTATTCGTGACCCTTCTTTTGCAATGATAGATGACCCACAATTGAGGTTTCTTAGCACAATGAGAGCAGTCCAAGGTGCACTAATTGTAGCATCCAGCATACAAATTATTTTGGGATTTAGTCAAATATGGGCTATTTGTTCTAGATTTTTCAGCCCACTTGGAATGGTTCCAGTAATTACATTAGTTGGTTTTGGATTATTTGACAGAGGTTTCCCTATGGTGGGGACTTGTGTTGAAATTGGAATTCCCATGCTAATCTTGTTTGTAGTGTTCTCTCAGTACTTGAAAAATTTTCAGACAAGACAAGTACCGATACTAGAGAGATTTGCTCTACTCATAACAACCACAGTGATATGGGCATATGCACATCTATTGACAGCGAGTGGAGCATACAAACACCGGCCGGATGTAACTCAACATAGTTGTAGGACAGACAGGGCTAATCTCATTTCTTCCGCTCCTTGGATAAAGATTCCATACCCTCTTGAGTGGGGTGCTCCTACATTTGATGCTGGCCACTCTTTTGGAATGATGGCTGCTGTTTTAGTCTCATTAGTTGAGTCAACTGGAGCATTCAAAGCTGCATCGCGTCTAGCAAGTGCCACACCACCGCCTGCTCATGTCCTGAGCCGTGGTATCGGTTGGCAGGGAATTGGAATTTTGCTGAATGGCCTTTTCGGAACACTAACCGGTTCAACAGTTTCTGTAGAGAATGTAGGACTCCTAGGAAGCAACCGTGTTGGCAGCCGAAGGGTTATTCAAGTTTCTGCTGGATTTATGATATTCTTTGCAATGTTAGGTAAATTTGGAGCTTTATTTGCATCAATACCATTCCCTATTTTTGCTGCTATATACTGTGTTTTGTTTGGTCTTGTAGCTTCTGTAGGGCTATCATTTTTGCAATTCACCAACATGAATTCAATGAGGAACCTCTTTATCACTGGTGTTGCCCTTTTCTTAGGCTTGTCTATTCCTGAATATTTCAGAGAATACACTATCAGGGCCTTTCATGGTCCTGCTCATACCAAAGCTGGATGGTTCAATGACTTCCTGAATACCATCTTCTATTCTTCACCAACTGTTGCATTGATTATTGCTGTGTTCTTGGACAATACTCTTGACTATAAGGACAGTGCCAAAGATAGAGGAATGCCATGGTGGGCTAAGTTTAGAACATTTAAAGCAGATAGCAGAAATGAAGAGTTCTACTCTCTTCCTTTCAATCTCAACCGCTTCTTTCCTCCATCATAG |
| Protein sequence |
>Msa1267930 MAAIKPEEISHSPMDQLQGLEYCIDSNPSWVETILLGFQHYILALGTAVMIPSFLVPSMGGNDDDKVRVVQTLLFVEGINTLLQTLFGTRLPTVIGGSYAFMVPIVSIIRDPSFAMIDDPQLRFLSTMRAVQGALIVASSIQIILGFSQIWAICSRFFSPLGMVPVITLVGFGLFDRGFPMVGTCVEIGIPMLILFVVFSQYLKNFQTRQVPILERFALLITTTVIWAYAHLLTASGAYKHRPDVTQHSCRTDRANLISSAPWIKIPYPLEWGAPTFDAGHSFGMMAAVLVSLVESTGAFKAASRLASATPPPAHVLSRGIGWQGIGILLNGLFGTLTGSTVSVENVGLLGSNRVGSRRVIQVSAGFMIFFAMLGKFGALFASIPFPIFAAIYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSIPEYFREYTIRAFHGPAHTKAGWFNDFLNTIFYSSPTVALIIAVFLDNTLDYKDSAKDRGMPWWAKFRTFKADSRNEEFYSLPFNLNRFFPPS* |
