AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1066960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066960 MtrunA17_Chr7g0247891 87.870 338 20 1 1 338 1 317 0.0 595
Msa1066960 MtrunA17_Chr2g0290951 83.333 252 22 1 1 252 1 232 4.40e-147 414
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066960 AT2G45980.1 39.412 340 120 11 1 338 1 256 3.93e-58 189
Msa1066960 AT4G00355.3 39.339 333 125 11 6 334 3 262 8.09e-56 183
Msa1066960 AT4G00355.2 39.339 333 125 11 6 334 3 262 8.09e-56 183
Msa1066960 AT4G00355.1 39.339 333 125 11 6 334 3 262 8.09e-56 183
Msa1066960 AT4G00355.4 41.489 282 109 10 6 283 3 232 4.11e-48 163

Find 95 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATTTACATATGATATT+TGG 0.188969 7_2:-61118336 None:intergenic
AGTGAGATTCAGAATGAATT+TGG 0.214211 7_2:+61116818 Msa1066960:CDS
GGCACAACTCTACCAGATTT+TGG 0.228578 7_2:+61117013 Msa1066960:CDS
ATCTCTCAACAAATCATATA+AGG 0.316050 7_2:+61117856 Msa1066960:CDS
GAAGAAAACTTGACATTAGA+AGG 0.322875 7_2:+61116902 Msa1066960:CDS
GGCTACTACCATATGGTAAA+AGG 0.325013 7_2:+61118072 Msa1066960:three_prime_UTR
CTCCTTTGCATGAGCATAGA+TGG 0.333088 7_2:-61117284 None:intergenic
GGACCTACAGCTAGAGTAAA+AGG 0.349922 7_2:-61118398 None:intergenic
GAATAACGGAATTCATAGTT+TGG 0.354381 7_2:-61118200 None:intergenic
CAGATCTTCCTTGCGGAGCT+TGG 0.354733 7_2:+61117242 Msa1066960:CDS
ATCAACAGATTATCTATTCA+TGG 0.363088 7_2:+61116974 Msa1066960:CDS
AACTGCCAATTCCACATAAC+AGG 0.379067 7_2:-61118307 None:intergenic
CATCGTGTCGCGTTCATCTC+TGG 0.381704 7_2:+61115606 Msa1066960:five_prime_UTR
TGTCTCCTGCTCTGCTGATT+CGG 0.382713 7_2:-61117155 None:intergenic
AGCAGCAATGTCTGTTTCAC+TGG 0.393072 7_2:-61117092 None:intergenic
CAAAGGAGGCAAATACATTA+TGG 0.393151 7_2:+61117299 Msa1066960:CDS
AGAAGTGCAGGTTTGATACT+AGG 0.393998 7_2:+61118162 Msa1066960:three_prime_UTR
ATTATTCATTCATATGTTAC+CGG 0.396888 7_2:-61115481 None:intergenic
TTCATCCTTTCCACTAGGCT+TGG 0.405829 7_2:-61116882 None:intergenic
AAACTTGACATTAGAAGGAT+TGG 0.407672 7_2:+61116907 Msa1066960:CDS
ATCTTCCTTGCGGAGCTTGG+TGG 0.419362 7_2:+61117245 Msa1066960:CDS
TTTGACAGTGATAAATCATC+AGG 0.420814 7_2:-61117187 None:intergenic
CACATCTTTGAGTCGAATCA+TGG 0.436493 7_2:-61117977 None:intergenic
GTGTTCCTGTTATGTGGAAT+TGG 0.445145 7_2:+61118302 Msa1066960:three_prime_UTR
TATGTCAGAGAGGTTGAGAA+TGG 0.449253 7_2:+61118124 Msa1066960:three_prime_UTR
TATTGTGTGTTCCTGTTATG+TGG 0.450636 7_2:+61118296 Msa1066960:three_prime_UTR
AAAGAGCTTTACAACTTAAG+TGG 0.462236 7_2:+61117383 Msa1066960:CDS
CGGAATTCATAGTTTGGTAA+AGG 0.463600 7_2:-61118194 None:intergenic
TCAACTTATGCAGCTGCTCC+TGG 0.470344 7_2:+61116650 Msa1066960:CDS
GGATGTGTCAAAGGAGTTTG+AGG 0.473894 7_2:+61116928 Msa1066960:CDS
ATGTAACGTTTAAGAAGTGC+AGG 0.475847 7_2:+61118150 Msa1066960:three_prime_UTR
GCTTGGTCAACGCTGGCGAC+AGG 0.477014 7_2:+61117360 Msa1066960:CDS
AGAGATTTATGTGTCTGTAT+AGG 0.478008 7_2:-61117839 None:intergenic
GAAGTGACCAGACATGAATA+AGG 0.494471 7_2:-61116747 None:intergenic
AATATATAATGTTTGAATAA+CGG 0.500958 7_2:-61118214 None:intergenic
GTCTTCTCGCGGTAATGACT+GGG 0.502839 7_2:+61116607 Msa1066960:CDS
TTCATTGCAGCAACAGTGAT+GGG 0.502883 7_2:+61117328 Msa1066960:CDS
AACTCAACTTCACGTGGACC+AGG 0.507089 7_2:-61116668 None:intergenic
TGTTTCCACAGGCAAGGAGC+AGG 0.508812 7_2:+61117944 Msa1066960:intron
CTCAAAGATGTGATCGTTGG+TGG 0.510999 7_2:+61117988 Msa1066960:CDS
TTATGAAGACAAAAGATGGC+AGG 0.513000 7_2:+61116563 Msa1066960:intron
TATTCATGGAAAACAGTTTG+AGG 0.519571 7_2:+61116988 Msa1066960:CDS
GGAAGAGCTTTCTCTGATCA+AGG 0.520300 7_2:-61118025 None:intergenic
TGATCAAGGAGCCACGGCGG+TGG 0.522980 7_2:-61118011 None:intergenic
TTCATGCTTACTTGGTGGAA+AGG 0.529697 7_2:-61116771 None:intergenic
TCAAACGCCTTATTCATGTC+TGG 0.529833 7_2:+61116740 Msa1066960:CDS
ATGGCAGGAACTGAAGATGA+AGG 0.531057 7_2:+61116578 Msa1066960:CDS
TGCAGCAACAGTGATGGGTT+TGG 0.532584 7_2:+61117333 Msa1066960:CDS
TTTCATTGCAGCAACAGTGA+TGG 0.533829 7_2:+61117327 Msa1066960:CDS
AACAACCGAATCAGCAGAGC+AGG 0.534322 7_2:+61117150 Msa1066960:CDS
TAAGCATGAAAACTTGCCAT+TGG 0.534678 7_2:+61116784 Msa1066960:CDS
AGAAGGATTGGATGTGTCAA+AGG 0.536048 7_2:+61116919 Msa1066960:CDS
ATCAGCAGAGCAGGAGACAA+AGG 0.541008 7_2:+61117159 Msa1066960:CDS
CAAAGATATCGCCTCTGTAG+AGG 0.545508 7_2:+61116844 Msa1066960:CDS
TGAAGGTGGCTACTACCATA+TGG 0.546356 7_2:+61118065 Msa1066960:three_prime_UTR
CGCTTCCACCAAGCTCCGCA+AGG 0.554434 7_2:-61117250 None:intergenic
ATGTCAGAGAGGTTGAGAAT+GGG 0.554482 7_2:+61118125 Msa1066960:three_prime_UTR
CATGGAAAACAGTTTGAGGA+AGG 0.557886 7_2:+61116992 Msa1066960:CDS
GCTCCGCAAGGAAGATCTGA+AGG 0.560737 7_2:-61117238 None:intergenic
GTGATGGGTTTGGTGATGCT+TGG 0.561745 7_2:+61117343 Msa1066960:CDS
TCCACGTGAAGTTGAGTTGA+AGG 0.562439 7_2:+61116673 Msa1066960:CDS
TGGTGATGCTTGGTCAACGC+TGG 0.565434 7_2:+61117353 Msa1066960:CDS
TTATGTGTCTGTATAGGATG+AGG 0.566846 7_2:-61117833 None:intergenic
TGCGGAGCTTGGTGGAAGCG+AGG 0.567411 7_2:+61117253 Msa1066960:CDS
ACATCTTTGAGTCGAATCAT+GGG 0.569536 7_2:-61117976 None:intergenic
CGACTCAAAGATGTGATCGT+TGG 0.574960 7_2:+61117985 Msa1066960:CDS
CATCTATGCTCATGCAAAGG+AGG 0.578296 7_2:+61117285 Msa1066960:CDS
TGGCAGGAACTGAAGATGAA+GGG 0.579156 7_2:+61116579 Msa1066960:CDS
GAAATTTAACTCGGTGAAGG+TGG 0.579228 7_2:+61118051 Msa1066960:exon
GCAGCAATGTCTGTTTCACT+GGG 0.581872 7_2:-61117091 None:intergenic
TGAGTTGAAGGATGATGACA+AGG 0.594626 7_2:+61116685 Msa1066960:CDS
AGCGACCAAGCCTAGTGGAA+AGG 0.597200 7_2:+61116877 Msa1066960:CDS
GGCAGGAACTGAAGATGAAG+GGG 0.599526 7_2:+61116580 Msa1066960:CDS
GCTGAAATTTAACTCGGTGA+AGG 0.601929 7_2:+61118048 Msa1066960:exon
TCCTTCAACTCAACTTCACG+TGG 0.602249 7_2:-61116674 None:intergenic
CTCCATCTATGCTCATGCAA+AGG 0.603944 7_2:+61117282 Msa1066960:CDS
GCAAGTCAGTATTTCTGATG+AGG 0.612309 7_2:+61117405 Msa1066960:CDS
TCACTATAGTCAGTTTCCAA+TGG 0.627762 7_2:-61116800 None:intergenic
TCTTCCGCTGAAATTTAACT+CGG 0.629458 7_2:+61118042 Msa1066960:exon
AACCCTTCAGATCTTCCTTG+CGG 0.630132 7_2:+61117235 Msa1066960:CDS
CGAAAAGTTGTATGTCAGAG+AGG 0.635492 7_2:+61118114 Msa1066960:three_prime_UTR
GAAGAAGCGACCAAGCCTAG+TGG 0.639637 7_2:+61116872 Msa1066960:CDS
ATCGTTGGTGGCCACCGCCG+TGG 0.649963 7_2:+61118000 Msa1066960:CDS
ACGCGACACGATGTTCGTAG+TGG 0.655563 7_2:-61115596 None:intergenic
CTCTGATCAAGGAGCCACGG+CGG 0.656435 7_2:-61118014 None:intergenic
TTCTCGCGGTAATGACTGGG+AGG 0.656632 7_2:+61116610 Msa1066960:CDS
TTTCTCTGATCAAGGAGCCA+CGG 0.661567 7_2:-61118017 None:intergenic
TTCACCGAGTTAAATTTCAG+CGG 0.667738 7_2:-61118046 None:intergenic
ATGTATTATGTATATCGATG+AGG 0.677515 7_2:-61115557 None:intergenic
CTCCGCAAGGAAGATCTGAA+GGG 0.679694 7_2:-61117237 None:intergenic
GAAGGGGAAAAGTCTTCTCG+CGG 0.683817 7_2:+61116596 Msa1066960:CDS
CTTTGCATGAGCATAGATGG+AGG 0.694461 7_2:-61117281 None:intergenic
TTGAAGGATGATGACAAGGA+AGG 0.695939 7_2:+61116689 Msa1066960:CDS
TTCTTCACGAGCCTCTACAG+AGG 0.708502 7_2:-61116855 None:intergenic
CAGCAATGTCTGTTTCACTG+GGG 0.782536 7_2:-61117090 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGTTATTTTAATTTAATATT+AGG + chr7_2:61115963-61115982 Msa1066960:intron 10.0%
!! AATATATAATGTTTGAATAA+CGG - chr7_2:61118217-61118236 None:intergenic 10.0%
!! TATTTATATTCATTCTTCTA+AGG + chr7_2:61117430-61117449 Msa1066960:intron 15.0%
!! CTTAATTTACATATGATATT+TGG - chr7_2:61118339-61118358 None:intergenic 15.0%
!!! TAACTATTTGCATGAAATTT+TGG + chr7_2:61116024-61116043 Msa1066960:intron 20.0%
!! AAACAAATACAATTATGCAA+GGG - chr7_2:61116469-61116488 None:intergenic 20.0%
!! AAAACAAATACAATTATGCA+AGG - chr7_2:61116470-61116489 None:intergenic 20.0%
!!! TTTTTTATGAAGACAAAAGA+TGG + chr7_2:61116559-61116578 Msa1066960:intron 20.0%
!! TTTGTTAAAAATGAAGCATA+AGG + chr7_2:61117468-61117487 Msa1066960:intron 20.0%
!! TATAATGTTATGCATAAAGA+TGG + chr7_2:61117562-61117581 Msa1066960:intron 20.0%
!!! TACTTTAAAAATGAAGGTTA+TGG + chr7_2:61117742-61117761 Msa1066960:intron 20.0%
!!! ACTTTAAAAATGAAGGTTAT+GGG + chr7_2:61117743-61117762 Msa1066960:intron 20.0%
!!! GCAATGTAATATTTATTTTG+TGG + chr7_2:61117776-61117795 Msa1066960:intron 20.0%
! ATGTATTATGTATATCGATG+AGG - chr7_2:61115560-61115579 None:intergenic 25.0%
!! TTCTTTATTTGTGCTGTTAT+TGG + chr7_2:61115762-61115781 Msa1066960:intron 25.0%
!!! TGTTGTTATTTGAATTCAGT+GGG + chr7_2:61115843-61115862 Msa1066960:intron 25.0%
! AAATAGTTAGATCAATCTCT+GGG - chr7_2:61116013-61116032 None:intergenic 25.0%
!!! GGTTATTTTAGTTTTTTCAG+TGG + chr7_2:61116045-61116064 Msa1066960:intron 25.0%
! TACAAGAAAACTACTACTTT+AGG - chr7_2:61116391-61116410 None:intergenic 25.0%
! ATCAACAGATTATCTATTCA+TGG + chr7_2:61116974-61116993 Msa1066960:CDS 25.0%
! TATGCATAACATTATAAACG+AGG - chr7_2:61117558-61117577 None:intergenic 25.0%
!! ATTTTTACTAGCACATCATA+AGG + chr7_2:61117645-61117664 Msa1066960:intron 25.0%
!! TACTCGTACTTTAAAAATGA+AGG + chr7_2:61117736-61117755 Msa1066960:intron 25.0%
! ATCTCTCAACAAATCATATA+AGG + chr7_2:61117856-61117875 Msa1066960:CDS 25.0%
!! GTGTTGTTATTTGAATTCAG+TGG + chr7_2:61115842-61115861 Msa1066960:intron 30.0%
! GTTGATGTGAATTTTGTGAT+TGG + chr7_2:61115908-61115927 Msa1066960:intron 30.0%
! TAGGTCATGTTTTCACTTAT+AGG + chr7_2:61115942-61115961 Msa1066960:intron 30.0%
CAAATAGTTAGATCAATCTC+TGG - chr7_2:61116014-61116033 None:intergenic 30.0%
AGTATAACCTTGTAATATCC+CGG + chr7_2:61116072-61116091 Msa1066960:intron 30.0%
! TCTGCGATTACGATTTTTAA+GGG - chr7_2:61116171-61116190 None:intergenic 30.0%
! TTCTGCGATTACGATTTTTA+AGG - chr7_2:61116172-61116191 None:intergenic 30.0%
! AACTACTACTTTAGGAAGAA+AGG - chr7_2:61116383-61116402 None:intergenic 30.0%
AGTGAGATTCAGAATGAATT+TGG + chr7_2:61116818-61116837 Msa1066960:CDS 30.0%
GAAGAAAACTTGACATTAGA+AGG + chr7_2:61116902-61116921 Msa1066960:CDS 30.0%
AAACTTGACATTAGAAGGAT+TGG + chr7_2:61116907-61116926 Msa1066960:CDS 30.0%
TATTCATGGAAAACAGTTTG+AGG + chr7_2:61116988-61117007 Msa1066960:CDS 30.0%
! TTTTCAGTCAAACCAAAATC+TGG - chr7_2:61117028-61117047 None:intergenic 30.0%
!!! TTTTGGTTTGACTGAAAAAG+AGG + chr7_2:61117030-61117049 Msa1066960:CDS 30.0%
! GGAAAGAATTGTATTTTGCA+GGG - chr7_2:61117072-61117091 None:intergenic 30.0%
TTTGACAGTGATAAATCATC+AGG - chr7_2:61117190-61117209 None:intergenic 30.0%
AAAGAGCTTTACAACTTAAG+TGG + chr7_2:61117383-61117402 Msa1066960:CDS 30.0%
!! GGTTGATAGTGTCAATTATT+TGG + chr7_2:61117583-61117602 Msa1066960:intron 30.0%
AGAGATTTATGTGTCTGTAT+AGG - chr7_2:61117842-61117861 None:intergenic 30.0%
! GAATAACGGAATTCATAGTT+TGG - chr7_2:61118203-61118222 None:intergenic 30.0%
!! TAATGAAGTGAGTGTTTTGA+TGG + chr7_2:61118360-61118379 Msa1066960:three_prime_UTR 30.0%
GTGATTGGAGTGAAAGAAAT+AGG + chr7_2:61115923-61115942 Msa1066960:intron 35.0%
! TTAGGGGCATGAAAATTTAG+AGG + chr7_2:61115981-61116000 Msa1066960:intron 35.0%
GTATAACCTTGTAATATCCC+GGG + chr7_2:61116073-61116092 Msa1066960:intron 35.0%
TTCTACAAACATTTCCAGGA+GGG - chr7_2:61116110-61116129 None:intergenic 35.0%
ATTCTACAAACATTTCCAGG+AGG - chr7_2:61116111-61116130 None:intergenic 35.0%
TCCTGGAAATGTTTGTAGAA+TGG + chr7_2:61116110-61116129 Msa1066960:intron 35.0%
ACCATTCTACAAACATTTCC+AGG - chr7_2:61116114-61116133 None:intergenic 35.0%
ATTCCTTGTGTAAATAGGCA+CGG - chr7_2:61116264-61116283 None:intergenic 35.0%
GAGTCATTCCTTGTGTAAAT+AGG - chr7_2:61116269-61116288 None:intergenic 35.0%
TTATGAAGACAAAAGATGGC+AGG + chr7_2:61116563-61116582 Msa1066960:intron 35.0%
! AAGTTTTCATGCTTACTTGG+TGG - chr7_2:61116779-61116798 None:intergenic 35.0%
TAAGCATGAAAACTTGCCAT+TGG + chr7_2:61116784-61116803 Msa1066960:CDS 35.0%
TCACTATAGTCAGTTTCCAA+TGG - chr7_2:61116803-61116822 None:intergenic 35.0%
! TTTTCTTCATCCTTTCCACT+AGG - chr7_2:61116890-61116909 None:intergenic 35.0%
! GGGAAAGAATTGTATTTTGC+AGG - chr7_2:61117073-61117092 None:intergenic 35.0%
!!! AGGGTTTTGTTTGTCATCTT+TGG - chr7_2:61117221-61117240 None:intergenic 35.0%
CAAAGGAGGCAAATACATTA+TGG + chr7_2:61117299-61117318 Msa1066960:CDS 35.0%
!! ATGAGTTTGTTGTACACAAC+TGG - chr7_2:61117627-61117646 None:intergenic 35.0%
TTATGTGTCTGTATAGGATG+AGG - chr7_2:61117836-61117855 None:intergenic 35.0%
ACATCTTTGAGTCGAATCAT+GGG - chr7_2:61117979-61117998 None:intergenic 35.0%
TCTTCCGCTGAAATTTAACT+CGG + chr7_2:61118042-61118061 Msa1066960:exon 35.0%
TTCACCGAGTTAAATTTCAG+CGG - chr7_2:61118049-61118068 None:intergenic 35.0%
!! GCTTTTAGCCTTTTACCATA+TGG - chr7_2:61118083-61118102 None:intergenic 35.0%
! ATGTAACGTTTAAGAAGTGC+AGG + chr7_2:61118150-61118169 Msa1066960:three_prime_UTR 35.0%
CGGAATTCATAGTTTGGTAA+AGG - chr7_2:61118197-61118216 None:intergenic 35.0%
TATTGTGTGTTCCTGTTATG+TGG + chr7_2:61118296-61118315 Msa1066960:three_prime_UTR 35.0%
! TTTCCTTTTACTCTAGCTGT+AGG + chr7_2:61118395-61118414 Msa1066960:three_prime_UTR 35.0%
GCTGTTATTGGATCTTTCTG+TGG + chr7_2:61115774-61115793 Msa1066960:intron 40.0%
! TGAGTTGAAGGATGATGACA+AGG + chr7_2:61116685-61116704 Msa1066960:CDS 40.0%
TTGAAGGATGATGACAAGGA+AGG + chr7_2:61116689-61116708 Msa1066960:CDS 40.0%
! TCAAACGCCTTATTCATGTC+TGG + chr7_2:61116740-61116759 Msa1066960:CDS 40.0%
GAAGTGACCAGACATGAATA+AGG - chr7_2:61116750-61116769 None:intergenic 40.0%
! TTCATGCTTACTTGGTGGAA+AGG - chr7_2:61116774-61116793 None:intergenic 40.0%
! GGCAAGTTTTCATGCTTACT+TGG - chr7_2:61116782-61116801 None:intergenic 40.0%
AGAAGGATTGGATGTGTCAA+AGG + chr7_2:61116919-61116938 Msa1066960:CDS 40.0%
CATGGAAAACAGTTTGAGGA+AGG + chr7_2:61116992-61117011 Msa1066960:CDS 40.0%
TTTCATTGCAGCAACAGTGA+TGG + chr7_2:61117327-61117346 Msa1066960:CDS 40.0%
TTCATTGCAGCAACAGTGAT+GGG + chr7_2:61117328-61117347 Msa1066960:CDS 40.0%
! GCAAGTCAGTATTTCTGATG+AGG + chr7_2:61117405-61117424 Msa1066960:CDS 40.0%
CCTGTGGAAACAATCACTAT+TGG - chr7_2:61117936-61117955 None:intergenic 40.0%
CCAATAGTGATTGTTTCCAC+AGG + chr7_2:61117933-61117952 Msa1066960:intron 40.0%
CACATCTTTGAGTCGAATCA+TGG - chr7_2:61117980-61117999 None:intergenic 40.0%
! GCTGAAATTTAACTCGGTGA+AGG + chr7_2:61118048-61118067 Msa1066960:exon 40.0%
! GAAATTTAACTCGGTGAAGG+TGG + chr7_2:61118051-61118070 Msa1066960:exon 40.0%
GGCTACTACCATATGGTAAA+AGG + chr7_2:61118072-61118091 Msa1066960:three_prime_UTR 40.0%
CGAAAAGTTGTATGTCAGAG+AGG + chr7_2:61118114-61118133 Msa1066960:three_prime_UTR 40.0%
TATGTCAGAGAGGTTGAGAA+TGG + chr7_2:61118124-61118143 Msa1066960:three_prime_UTR 40.0%
ATGTCAGAGAGGTTGAGAAT+GGG + chr7_2:61118125-61118144 Msa1066960:three_prime_UTR 40.0%
! AGAAGTGCAGGTTTGATACT+AGG + chr7_2:61118162-61118181 Msa1066960:three_prime_UTR 40.0%
GTGTTCCTGTTATGTGGAAT+TGG + chr7_2:61118302-61118321 Msa1066960:three_prime_UTR 40.0%
AACTGCCAATTCCACATAAC+AGG - chr7_2:61118310-61118329 None:intergenic 40.0%
!!! TGAAGTGAGTGTTTTGATGG+CGG + chr7_2:61118363-61118382 Msa1066960:three_prime_UTR 40.0%
TAACCTTGTAATATCCCGGG+CGG + chr7_2:61116076-61116095 Msa1066960:intron 45.0%
TCACCGTGCCTATTTACACA+AGG + chr7_2:61116258-61116277 Msa1066960:intron 45.0%
CAATTATGCAAGGGAGAGCA+GGG - chr7_2:61116460-61116479 None:intergenic 45.0%
ACAATTATGCAAGGGAGAGC+AGG - chr7_2:61116461-61116480 None:intergenic 45.0%
ATGGCAGGAACTGAAGATGA+AGG + chr7_2:61116578-61116597 Msa1066960:CDS 45.0%
TGGCAGGAACTGAAGATGAA+GGG + chr7_2:61116579-61116598 Msa1066960:CDS 45.0%
!! TCCACGTGAAGTTGAGTTGA+AGG + chr7_2:61116673-61116692 Msa1066960:CDS 45.0%
TCCTTCAACTCAACTTCACG+TGG - chr7_2:61116677-61116696 None:intergenic 45.0%
CAAAGATATCGCCTCTGTAG+AGG + chr7_2:61116844-61116863 Msa1066960:CDS 45.0%
TTCATCCTTTCCACTAGGCT+TGG - chr7_2:61116885-61116904 None:intergenic 45.0%
GGATGTGTCAAAGGAGTTTG+AGG + chr7_2:61116928-61116947 Msa1066960:CDS 45.0%
! GGCACAACTCTACCAGATTT+TGG + chr7_2:61117013-61117032 Msa1066960:CDS 45.0%
CAGCAATGTCTGTTTCACTG+GGG - chr7_2:61117093-61117112 None:intergenic 45.0%
GCAGCAATGTCTGTTTCACT+GGG - chr7_2:61117094-61117113 None:intergenic 45.0%
AGCAGCAATGTCTGTTTCAC+TGG - chr7_2:61117095-61117114 None:intergenic 45.0%
AACCCTTCAGATCTTCCTTG+CGG + chr7_2:61117235-61117254 Msa1066960:CDS 45.0%
CTTTGCATGAGCATAGATGG+AGG - chr7_2:61117284-61117303 None:intergenic 45.0%
CTCCATCTATGCTCATGCAA+AGG + chr7_2:61117282-61117301 Msa1066960:CDS 45.0%
CTCCTTTGCATGAGCATAGA+TGG - chr7_2:61117287-61117306 None:intergenic 45.0%
CATCTATGCTCATGCAAAGG+AGG + chr7_2:61117285-61117304 Msa1066960:CDS 45.0%
AGTGATTGTTTCCACAGGCA+AGG + chr7_2:61117938-61117957 Msa1066960:intron 45.0%
CGACTCAAAGATGTGATCGT+TGG + chr7_2:61117985-61118004 Msa1066960:CDS 45.0%
CTCAAAGATGTGATCGTTGG+TGG + chr7_2:61117988-61118007 Msa1066960:CDS 45.0%
TTTCTCTGATCAAGGAGCCA+CGG - chr7_2:61118020-61118039 None:intergenic 45.0%
GGAAGAGCTTTCTCTGATCA+AGG - chr7_2:61118028-61118047 None:intergenic 45.0%
TGAAGGTGGCTACTACCATA+TGG + chr7_2:61118065-61118084 Msa1066960:three_prime_UTR 45.0%
!!! GAAGTGAGTGTTTTGATGGC+GGG + chr7_2:61118364-61118383 Msa1066960:three_prime_UTR 45.0%
GGACCTACAGCTAGAGTAAA+AGG - chr7_2:61118401-61118420 None:intergenic 45.0%
!!! TTTTTATACTATTATTATTA+TGG - chr7_2:61115520-61115539 None:intergenic 5.0%
!!! GTTATTTTAATTTAATATTA+GGG + chr7_2:61115964-61115983 Msa1066960:intron 5.0%
!!! TTATTTTAATTTAATATTAG+GGG + chr7_2:61115965-61115984 Msa1066960:intron 5.0%
AATCCGCCCGGGATATTACA+AGG - chr7_2:61116082-61116101 None:intergenic 50.0%
! AGGAGGGTTTAAAATCCGCC+CGG - chr7_2:61116094-61116113 None:intergenic 50.0%
ACAAGCATGTGCAGGGTCAT+TGG - chr7_2:61116336-61116355 None:intergenic 50.0%
ACAGCTCACAAGCATGTGCA+GGG - chr7_2:61116343-61116362 None:intergenic 50.0%
GAGCAGGGCTCGACAAAAAA+AGG - chr7_2:61116445-61116464 None:intergenic 50.0%
GGCAGGAACTGAAGATGAAG+GGG + chr7_2:61116580-61116599 Msa1066960:CDS 50.0%
GAAGGGGAAAAGTCTTCTCG+CGG + chr7_2:61116596-61116615 Msa1066960:CDS 50.0%
AGTCTTCTCGCGGTAATGAC+TGG + chr7_2:61116606-61116625 Msa1066960:CDS 50.0%
GTCTTCTCGCGGTAATGACT+GGG + chr7_2:61116607-61116626 Msa1066960:CDS 50.0%
TCAACTTATGCAGCTGCTCC+TGG + chr7_2:61116650-61116669 Msa1066960:CDS 50.0%
AACTCAACTTCACGTGGACC+AGG - chr7_2:61116671-61116690 None:intergenic 50.0%
TTCTTCACGAGCCTCTACAG+AGG - chr7_2:61116858-61116877 None:intergenic 50.0%
AACAACCGAATCAGCAGAGC+AGG + chr7_2:61117150-61117169 Msa1066960:CDS 50.0%
! TGTCTCCTGCTCTGCTGATT+CGG - chr7_2:61117158-61117177 None:intergenic 50.0%
ATCAGCAGAGCAGGAGACAA+AGG + chr7_2:61117159-61117178 Msa1066960:CDS 50.0%
CTCCGCAAGGAAGATCTGAA+GGG - chr7_2:61117240-61117259 None:intergenic 50.0%
!! TGCAGCAACAGTGATGGGTT+TGG + chr7_2:61117333-61117352 Msa1066960:CDS 50.0%
!! GTGATGGGTTTGGTGATGCT+TGG + chr7_2:61117343-61117362 Msa1066960:CDS 50.0%
CGCGACACGATGTTCGTAGT+GGG - chr7_2:61115598-61115617 None:intergenic 55.0%
ACGCGACACGATGTTCGTAG+TGG - chr7_2:61115599-61115618 None:intergenic 55.0%
CATCGTGTCGCGTTCATCTC+TGG + chr7_2:61115606-61115625 Msa1066960:five_prime_UTR 55.0%
! GGAGGGTTTAAAATCCGCCC+GGG - chr7_2:61116093-61116112 None:intergenic 55.0%
!! GGGCGGATTTTAAACCCTCC+TGG + chr7_2:61116093-61116112 Msa1066960:intron 55.0%
CACAGCTCACAAGCATGTGC+AGG - chr7_2:61116344-61116363 None:intergenic 55.0%
TTCTCGCGGTAATGACTGGG+AGG + chr7_2:61116610-61116629 Msa1066960:CDS 55.0%
GAAGAAGCGACCAAGCCTAG+TGG + chr7_2:61116872-61116891 Msa1066960:CDS 55.0%
AGCGACCAAGCCTAGTGGAA+AGG + chr7_2:61116877-61116896 Msa1066960:CDS 55.0%
GCTCCGCAAGGAAGATCTGA+AGG - chr7_2:61117241-61117260 None:intergenic 55.0%
CAGATCTTCCTTGCGGAGCT+TGG + chr7_2:61117242-61117261 Msa1066960:CDS 55.0%
ATCTTCCTTGCGGAGCTTGG+TGG + chr7_2:61117245-61117264 Msa1066960:CDS 55.0%
! TGGTGATGCTTGGTCAACGC+TGG + chr7_2:61117353-61117372 Msa1066960:CDS 55.0%
TGTTTCCACAGGCAAGGAGC+AGG + chr7_2:61117944-61117963 Msa1066960:intron 55.0%
AGCATCCTGCTCCTTGCCTG+TGG - chr7_2:61117952-61117971 None:intergenic 60.0%
CTCTGATCAAGGAGCCACGG+CGG - chr7_2:61118017-61118036 None:intergenic 60.0%
CGCTTCCACCAAGCTCCGCA+AGG - chr7_2:61117253-61117272 None:intergenic 65.0%
! TGCGGAGCTTGGTGGAAGCG+AGG + chr7_2:61117253-61117272 Msa1066960:CDS 65.0%
GCTTGGTCAACGCTGGCGAC+AGG + chr7_2:61117360-61117379 Msa1066960:CDS 65.0%
TGATCAAGGAGCCACGGCGG+TGG - chr7_2:61118014-61118033 None:intergenic 65.0%
!! ATCGTTGGTGGCCACCGCCG+TGG + chr7_2:61118000-61118019 Msa1066960:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 61115484 61118421 61115484 ID=Msa1066960;Name=Msa1066960
chr7_2 mRNA 61115484 61118421 61115484 ID=Msa1066960-mRNA-1;Parent=Msa1066960;Name=Msa1066960-mRNA-1;_AED=0.05;_eAED=0.05;_QI=151|0.33|0.75|0.75|0.66|0.75|4|362|338
chr7_2 exon 61115484 61115627 61115484 ID=Msa1066960-mRNA-1:exon:12237;Parent=Msa1066960-mRNA-1
chr7_2 exon 61116571 61117426 61116571 ID=Msa1066960-mRNA-1:exon:12238;Parent=Msa1066960-mRNA-1
chr7_2 exon 61117815 61117877 61117815 ID=Msa1066960-mRNA-1:exon:12239;Parent=Msa1066960-mRNA-1
chr7_2 exon 61117955 61118421 61117955 ID=Msa1066960-mRNA-1:exon:12240;Parent=Msa1066960-mRNA-1
chr7_2 five_prime_UTR 61115484 61115627 61115484 ID=Msa1066960-mRNA-1:five_prime_utr;Parent=Msa1066960-mRNA-1
chr7_2 five_prime_UTR 61116571 61116577 61116571 ID=Msa1066960-mRNA-1:five_prime_utr;Parent=Msa1066960-mRNA-1
chr7_2 CDS 61116578 61117426 61116578 ID=Msa1066960-mRNA-1:cds;Parent=Msa1066960-mRNA-1
chr7_2 CDS 61117815 61117877 61117815 ID=Msa1066960-mRNA-1:cds;Parent=Msa1066960-mRNA-1
chr7_2 CDS 61117955 61118059 61117955 ID=Msa1066960-mRNA-1:cds;Parent=Msa1066960-mRNA-1
chr7_2 three_prime_UTR 61118060 61118421 61118060 ID=Msa1066960-mRNA-1:three_prime_utr;Parent=Msa1066960-mRNA-1
Gene Sequence

>Msa1066960

ATGGCAGGAACTGAAGATGAAGGGGAAAAGTCTTCTCGCGGTAATGACTGGGAGGTTGTATCTCTCACAGCATCAACTTATGCAGCTGCTCCTGGTCCACGTGAAGTTGAGTTGAAGGATGATGACAAGGAAGGTGCATATGCAACAAATGAAGCTGAAACATCAAACGCCTTATTCATGTCTGGTCACTTCGCCTTTCCACCAAGTAAGCATGAAAACTTGCCATTGGAAACTGACTATAGTGAGATTCAGAATGAATTTGGAGACAAAGATATCGCCTCTGTAGAGGCTCGTGAAGAAGCGACCAAGCCTAGTGGAAAGGATGAAGAAAACTTGACATTAGAAGGATTGGATGTGTCAAAGGAGTTTGAGGATATGAAATATTATGATGAGAAAATCAACAGATTATCTATTCATGGAAAACAGTTTGAGGAAGGCACAACTCTACCAGATTTTGGTTTGACTGAAAAAGAGGAAAGTATATATCATAGCCCTGCAAAATACAATTCTTTCCCCAGTGAAACAGACATTGCTGCTGTGACAGTATACGATGAAAACGTAGTTGAGTCTGTAACAACCGAATCAGCAGAGCAGGAGACAAAGGTATCTCCTGATGATTTATCACTGTCAAATAACTCTTCCAAAGATGACAAACAAAACCCTTCAGATCTTCCTTGCGGAGCTTGGTGGAAGCGAGGAGTTGCCTCCATCTATGCTCATGCAAAGGAGGCAAATACATTATGGTCTGTTTTCATTGCAGCAACAGTGATGGGTTTGGTGATGCTTGGTCAACGCTGGCGACAGGAAAGAGCTTTACAACTTAAGTGGCAAGTCAGTATTTCTGATGAGAGCTCAACTTTTGAATTTCCTCATCCTATACAGACACATAAATCTCTCAACAAATCATATAAGGCAAGGAGCAGGATGCTTTCTCCCATGATTCGACTCAAAGATGTGATCGTTGGTGGCCACCGCCGTGGCTCCTTGATCAGAGAAAGCTCTTCCGCTGAAATTTAA
Protein sequence

>Msa1066960

MAGTEDEGEKSSRGNDWEVVSLTASTYAAAPGPREVELKDDDKEGAYATNEAETSNALFMSGHFAFPPSKHENLPLETDYSEIQNEFGDKDIASVEAREEATKPSGKDEENLTLEGLDVSKEFEDMKYYDEKINRLSIHGKQFEEGTTLPDFGLTEKEESIYHSPAKYNSFPSETDIAAVTVYDENVVESVTTESAEQETKVSPDDLSLSNNSSKDDKQNPSDLPCGAWWKRGVASIYAHAKEANTLWSVFIAATVMGLVMLGQRWRQERALQLKWQVSISDESSTFEFPHPIQTHKSLNKSYKARSRMLSPMIRLKDVIVGGHRRGSLIRESSSAEI*